AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01953

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01953.t1 MTR_2g027240 94.690 226 7 1 6 226 379 604 1.25e-154 442
MS.gene01953.t1 MTR_6g004720 67.568 222 56 4 6 226 424 630 6.96e-101 305
MS.gene01953.t1 MTR_7g033640 68.122 229 50 4 6 226 405 618 1.68e-99 301
MS.gene01953.t1 MTR_7g081665 66.376 229 65 5 6 226 537 761 2.53e-96 297
MS.gene01953.t1 MTR_8g006870 64.912 228 73 1 6 226 544 771 1.69e-94 292
MS.gene01953.t1 MTR_8g446990 60.563 213 78 4 18 226 319 529 1.04e-83 258
MS.gene01953.t1 MTR_2g062850 61.722 209 74 4 22 226 359 565 2.88e-82 256
MS.gene01953.t1 MTR_1g060790 60.000 225 76 5 7 226 644 859 2.33e-80 257
MS.gene01953.t1 MTR_3g010260 56.054 223 84 5 7 226 591 802 6.24e-75 241
MS.gene01953.t1 MTR_7g104180 64.674 184 60 3 8 188 667 848 4.85e-74 240
MS.gene01953.t1 MTR_2g085065 52.071 169 70 1 8 176 277 434 3.27e-52 175
MS.gene01953.t1 MTR_2g018990 49.143 175 76 3 12 183 261 425 2.78e-51 172
MS.gene01953.t1 MTR_4g113790 51.579 190 74 4 7 183 273 457 4.04e-51 172
MS.gene01953.t1 MTR_8g089420 50.898 167 69 3 20 183 267 423 9.76e-51 171
MS.gene01953.t1 MTR_4g061610 48.193 166 74 5 17 182 790 943 1.80e-42 153
MS.gene01953.t1 MTR_2g069990 44.970 169 89 2 53 217 286 454 3.84e-41 146
MS.gene01953.t1 MTR_2g095980 53.333 135 62 1 55 189 558 691 4.36e-40 145
MS.gene01953.t1 MTR_2g095980 52.941 136 63 1 54 189 812 946 6.08e-40 145
MS.gene01953.t1 MTR_2g095980 52.941 136 63 1 54 189 812 946 8.26e-40 145
MS.gene01953.t1 MTR_8g070530 50.370 135 66 1 54 188 775 908 5.12e-39 143
MS.gene01953.t1 MTR_2g098240 45.695 151 77 3 35 183 186 333 1.33e-32 122
MS.gene01953.t1 MTR_4g065080 47.967 123 60 2 62 183 250 369 1.67e-30 117
MS.gene01953.t1 MTR_8g011780 43.796 137 69 3 62 197 246 375 1.77e-30 117
MS.gene01953.t1 MTR_5g069000 41.600 125 71 2 62 185 947 1070 2.74e-25 103
MS.gene01953.t1 MTR_4g035835 41.600 125 71 2 62 185 943 1066 3.26e-25 103
MS.gene01953.t1 MTR_3g088715 41.071 112 62 1 56 167 19 126 4.48e-24 93.6
MS.gene01953.t1 MTR_7g053200 41.129 124 65 3 62 184 237 353 6.70e-23 95.9
MS.gene01953.t1 MTR_6g470940 42.105 114 64 2 62 174 1062 1174 7.72e-23 96.7
MS.gene01953.t1 MTR_3g104700 36.970 165 89 6 49 200 282 444 2.97e-22 94.7
MS.gene01953.t1 MTR_4g107280 37.069 116 70 2 62 176 210 323 2.90e-21 91.3
MS.gene01953.t1 MTR_4g107280 38.393 112 66 2 62 172 249 358 4.27e-21 90.9
MS.gene01953.t1 MTR_1g019340 39.456 147 78 6 36 174 273 416 3.86e-20 88.6
MS.gene01953.t1 MTR_1g019340 39.456 147 78 6 36 174 273 416 5.11e-20 88.2
MS.gene01953.t1 MTR_7g073520 42.857 112 61 2 61 171 300 409 1.27e-19 87.0
MS.gene01953.t1 MTR_1g442790 39.706 136 72 5 65 192 309 442 1.92e-18 84.0
MS.gene01953.t1 MTR_1g442790 39.706 136 72 5 65 192 309 442 2.14e-18 83.6
MS.gene01953.t1 MTR_7g097150 38.356 146 77 5 65 199 317 460 2.21e-18 83.6
MS.gene01953.t1 MTR_1g069735 38.028 142 78 5 65 198 317 456 7.16e-18 82.0
MS.gene01953.t1 MTR_1g069735 38.028 142 78 5 65 198 317 456 8.10e-18 82.0
MS.gene01953.t1 MTR_1g069735 38.028 142 78 5 65 198 317 456 8.24e-18 82.0
MS.gene01953.t1 MTR_2g040150 32.727 165 96 5 49 200 301 463 9.53e-18 81.6
MS.gene01953.t1 MTR_1g075880 37.324 142 79 4 65 198 315 454 2.43e-17 80.5
MS.gene01953.t1 MTR_8g040300 37.838 111 64 2 56 166 156 261 1.16e-16 77.0
MS.gene01953.t1 MTR_8g035890 38.679 106 61 1 62 167 167 268 1.58e-16 76.6
MS.gene01953.t1 MTR_3g110405 38.889 90 55 0 62 151 188 277 2.59e-16 76.3
MS.gene01953.t1 MTR_1g042700 40.650 123 68 4 65 185 314 433 1.37e-15 75.5
MS.gene01953.t1 MTR_6g087000 34.109 129 77 4 59 182 909 1034 5.07e-14 71.2
MS.gene01953.t1 MTR_1g048630 33.019 106 65 2 65 170 162 261 8.71e-14 70.5
MS.gene01953.t1 MTR_1g090917 34.307 137 87 2 47 182 75 209 2.70e-13 68.6
MS.gene01953.t1 MTR_1g090917 34.307 137 87 2 47 182 75 209 3.17e-13 68.2
MS.gene01953.t1 MTR_1g090917 34.307 137 87 2 47 182 200 334 5.91e-13 67.8
MS.gene01953.t1 MTR_1g090917 34.307 137 87 2 47 182 200 334 6.38e-13 67.8
MS.gene01953.t1 MTR_4g019410 34.615 104 62 3 65 166 177 276 1.50e-12 66.6
MS.gene01953.t1 MTR_4g019410 34.286 105 63 3 65 167 177 277 1.52e-12 66.6
MS.gene01953.t1 MTR_7g116390 35.922 103 59 3 67 166 176 274 2.75e-11 62.4
MS.gene01953.t1 MTR_1g080020 33.636 110 66 3 60 166 159 264 4.03e-11 61.6
MS.gene01953.t1 MTR_1g101630 38.462 104 57 3 66 167 202 300 4.04e-11 62.4
MS.gene01953.t1 MTR_4g132070 31.429 140 82 5 29 166 222 349 4.25e-11 62.4
MS.gene01953.t1 MTR_2g006330 33.333 111 65 3 61 166 78 184 8.39e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01953.t1 AT5G47750 76.623 231 39 3 5 226 362 586 2.06e-122 359
MS.gene01953.t1 AT4G26610 76.126 222 42 2 6 226 295 506 1.03e-110 327
MS.gene01953.t1 AT5G55910 75.771 227 41 5 5 226 281 498 9.95e-109 322
MS.gene01953.t1 AT5G55910 75.771 227 41 5 5 226 281 498 9.95e-109 322
MS.gene01953.t1 AT3G27580 63.717 226 77 2 6 226 353 578 3.04e-95 290
MS.gene01953.t1 AT3G27580 63.717 226 77 2 6 226 353 578 3.04e-95 290
MS.gene01953.t1 AT5G40030 59.821 224 79 6 6 226 284 499 9.97e-85 260
MS.gene01953.t1 AT1G79250 60.000 225 72 6 18 226 333 555 8.95e-83 257
MS.gene01953.t1 AT1G79250 60.000 225 72 6 18 226 333 555 8.95e-83 257
MS.gene01953.t1 AT1G79250 60.000 225 72 6 18 226 333 555 8.95e-83 257
MS.gene01953.t1 AT1G79250 60.000 225 72 6 18 226 333 555 8.95e-83 257
MS.gene01953.t1 AT2G44830 58.228 237 79 6 6 226 533 765 8.75e-80 254
MS.gene01953.t1 AT2G44830 58.228 237 79 6 6 226 533 765 8.75e-80 254
MS.gene01953.t1 AT2G44830 58.228 237 79 6 6 226 533 765 8.75e-80 254
MS.gene01953.t1 AT3G12690 67.935 184 52 3 18 201 364 540 1.83e-78 246
MS.gene01953.t1 AT3G12690 67.935 184 52 3 18 201 364 540 1.83e-78 246
MS.gene01953.t1 AT3G12690 67.935 184 52 3 18 201 364 540 1.83e-78 246
MS.gene01953.t1 AT1G16440 58.796 216 73 5 18 226 293 499 2.63e-75 236
MS.gene01953.t1 AT3G52890 58.257 218 70 5 7 208 717 929 1.80e-73 239
MS.gene01953.t1 AT3G52890 58.257 218 70 5 7 208 717 929 1.80e-73 239
MS.gene01953.t1 AT3G52890 58.257 218 70 5 7 208 717 929 1.80e-73 239
MS.gene01953.t1 AT3G52890 58.257 218 70 5 7 208 717 929 1.80e-73 239
MS.gene01953.t1 AT2G36350 54.911 224 85 5 7 226 738 949 7.45e-73 238
MS.gene01953.t1 AT5G03640 54.751 221 62 4 12 222 721 913 1.56e-69 229
MS.gene01953.t1 AT5G03640 54.751 221 62 4 12 222 721 913 1.56e-69 229
MS.gene01953.t1 AT3G44610 56.442 163 61 4 16 177 271 424 1.55e-56 186
MS.gene01953.t1 AT2G26700 45.455 220 74 6 7 184 268 483 7.97e-51 172
MS.gene01953.t1 AT2G34650 49.697 165 79 3 26 186 250 414 9.30e-47 160
MS.gene01953.t1 AT3G14370 50.658 152 62 2 53 197 282 427 3.60e-41 146
MS.gene01953.t1 AT5G58140 47.170 159 77 2 22 180 727 878 8.87e-41 148
MS.gene01953.t1 AT5G58140 47.170 159 77 2 22 180 727 878 8.87e-41 148
MS.gene01953.t1 AT5G58140 47.170 159 77 2 22 180 727 878 8.87e-41 148
MS.gene01953.t1 AT5G58140 47.170 159 77 2 22 180 727 878 8.87e-41 148
MS.gene01953.t1 AT5G58140 47.170 159 77 2 22 180 727 878 8.87e-41 148
MS.gene01953.t1 AT1G53700 50.000 148 73 1 53 199 286 433 2.81e-39 141
MS.gene01953.t1 AT5G58140 47.403 154 74 2 22 175 727 873 4.83e-39 143
MS.gene01953.t1 AT3G45780 52.713 129 60 1 54 182 841 968 1.35e-38 142
MS.gene01953.t1 AT3G45780 52.713 129 60 1 54 182 841 968 1.35e-38 142
MS.gene01953.t1 AT1G51170 43.125 160 80 4 26 183 208 358 1.47e-32 122
MS.gene01953.t1 AT3G25250 44.615 130 64 3 55 183 225 347 7.19e-31 117
MS.gene01953.t1 AT3G20830 48.780 123 59 2 62 183 240 359 7.83e-30 114
MS.gene01953.t1 AT4G13000 39.344 183 95 7 15 189 172 346 1.34e-28 110
MS.gene01953.t1 AT3G08730 46.018 113 57 3 60 171 285 394 3.13e-25 102
MS.gene01953.t1 AT3G08730 46.018 113 57 3 60 171 285 394 3.13e-25 102
MS.gene01953.t1 AT3G08720 45.133 113 58 3 60 171 291 400 2.44e-24 100
MS.gene01953.t1 AT3G08720 45.133 113 58 3 60 171 291 400 2.44e-24 100
MS.gene01953.t1 AT3G08720 45.133 113 58 3 60 171 291 400 2.44e-24 100
MS.gene01953.t1 AT3G08720 45.133 113 58 3 60 171 291 400 2.44e-24 100
MS.gene01953.t1 AT1G48490 41.481 135 72 3 43 171 983 1116 2.77e-24 100
MS.gene01953.t1 AT1G48490 41.481 135 72 3 43 171 983 1116 2.77e-24 100
MS.gene01953.t1 AT1G48490 41.481 135 72 3 43 171 983 1116 2.77e-24 100
MS.gene01953.t1 AT1G48490 41.481 135 72 3 43 171 984 1117 2.77e-24 100
MS.gene01953.t1 AT1G45160 40.000 155 85 5 65 213 854 1006 1.23e-23 99.4
MS.gene01953.t1 AT1G45160 40.000 155 85 5 65 213 854 1006 1.25e-23 99.0
MS.gene01953.t1 AT3G17850 40.351 114 66 2 62 174 1067 1179 1.79e-21 92.8
MS.gene01953.t1 AT5G62310 41.667 108 61 2 65 171 942 1048 2.39e-21 92.4
MS.gene01953.t1 AT5G04510 35.036 137 86 2 47 182 193 327 3.96e-19 85.9
MS.gene01953.t1 AT5G04510 35.036 137 86 2 47 182 193 327 4.24e-19 85.1
MS.gene01953.t1 AT3G10540 34.307 137 87 2 47 182 194 328 9.13e-19 84.7
MS.gene01953.t1 AT2G45490 41.176 102 56 1 65 166 176 273 1.11e-18 82.8
MS.gene01953.t1 AT1G30640 35.036 137 83 3 53 185 215 349 1.99e-18 83.6
MS.gene01953.t1 AT1G30640 35.036 137 83 3 53 185 308 442 2.58e-18 83.6
MS.gene01953.t1 AT1G30640 35.036 137 83 3 53 185 308 442 2.58e-18 83.6
MS.gene01953.t1 AT4G33080 34.375 160 91 5 49 195 282 440 4.91e-18 82.8
MS.gene01953.t1 AT1G03920 39.706 136 72 5 65 192 221 354 5.14e-18 82.4
MS.gene01953.t1 AT1G03920 39.706 136 72 5 65 192 334 467 5.25e-18 82.8
MS.gene01953.t1 AT1G03920 39.706 136 72 5 65 192 334 467 5.25e-18 82.8
MS.gene01953.t1 AT4G33080 34.375 160 91 5 49 195 282 440 5.31e-18 82.4
MS.gene01953.t1 AT5G09890 38.889 126 71 4 50 171 286 409 6.03e-18 82.4
MS.gene01953.t1 AT5G09890 38.889 126 71 4 50 171 286 409 6.44e-18 82.4
MS.gene01953.t1 AT2G20470 35.915 142 81 5 65 198 320 459 1.82e-17 80.9
MS.gene01953.t1 AT2G20470 35.915 142 81 5 65 198 320 459 1.99e-17 80.9
MS.gene01953.t1 AT2G20470 35.915 142 81 5 65 198 320 459 1.99e-17 80.9
MS.gene01953.t1 AT2G20050 38.793 116 65 3 62 174 931 1043 3.99e-17 80.1
MS.gene01953.t1 AT2G20050 38.793 116 65 3 62 174 934 1046 4.34e-17 80.1
MS.gene01953.t1 AT2G25880 37.143 105 62 1 62 166 144 244 6.12e-17 77.4
MS.gene01953.t1 AT2G25880 37.143 105 62 1 62 166 176 276 9.22e-17 77.4
MS.gene01953.t1 AT2G25880 37.143 105 62 1 62 166 189 289 1.09e-16 77.4
MS.gene01953.t1 AT4G32830 40.000 90 54 0 62 151 182 271 1.68e-16 77.0
MS.gene01953.t1 AT2G25880 37.037 108 64 1 59 166 234 337 2.12e-16 77.4
MS.gene01953.t1 AT3G23310 37.398 123 73 2 65 185 320 440 5.52e-16 76.6
MS.gene01953.t1 AT2G19400 36.719 128 75 4 49 171 293 419 1.55e-15 75.5
MS.gene01953.t1 AT4G14350 36.585 123 74 2 65 185 317 437 2.70e-15 74.7
MS.gene01953.t1 AT4G14350 36.585 123 74 2 65 185 317 437 2.73e-15 74.7
MS.gene01953.t1 AT4G14350 36.585 123 74 2 65 185 317 437 2.73e-15 74.7
MS.gene01953.t1 AT4G14350 36.585 123 74 2 65 185 317 437 2.73e-15 74.7
MS.gene01953.t1 AT1G50240 34.951 103 61 2 65 167 161 257 1.55e-13 69.7
MS.gene01953.t1 AT1G50240 34.951 103 61 2 65 167 161 257 1.55e-13 69.7
MS.gene01953.t1 AT2G45490 40.506 79 47 0 65 143 176 254 9.84e-13 65.9
MS.gene01953.t1 AT1G12580 37.864 103 57 3 67 167 206 303 7.17e-12 64.7
MS.gene01953.t1 AT3G61960 33.898 118 67 4 47 154 144 260 3.02e-11 62.8
MS.gene01953.t1 AT3G61960 33.898 118 67 4 47 154 144 260 3.12e-11 62.8
MS.gene01953.t1 AT1G08650 34.951 103 61 2 67 167 176 274 6.41e-11 61.2
MS.gene01953.t1 AT5G39440 29.936 157 98 5 68 220 178 326 6.62e-11 61.6

Find 65 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGGCAAAACTCCGTTTA+AGG 0.192811 2.2:+59995004 MS.gene01953:CDS
TTGCAGCAAGGGATCTTATC+AGG 0.245422 2.2:+59995102 MS.gene01953:CDS
TTGATTGCCAAGCTCGGTTT+TGG 0.289209 2.2:-59994823 None:intergenic
TTGTCAGAACCTTTCTGATT+AGG 0.293830 2.2:-59995338 None:intergenic
GCAGTTGATTGTTGGACTTA+TGG 0.319968 2.2:+59994960 MS.gene01953:CDS
AAGCTAACAACAGGTGCTTC+TGG 0.322983 2.2:-59995080 None:intergenic
AAACGGTGTGGCTCGGTTCC+CGG 0.333246 2.2:-59995030 None:intergenic
TTCTCAAACTCAACAGCCTT+TGG 0.340147 2.2:-59995251 None:intergenic
GAAAGGTTCTGACAATTATC+TGG 0.347201 2.2:+59995346 MS.gene01953:CDS
TTTCTATACGAGTTGTTGTT+TGG 0.373039 2.2:+59994987 MS.gene01953:CDS
TTTGGCAAAACTCCGTTTAA+GGG 0.411556 2.2:+59995005 MS.gene01953:CDS
CCTTCACCCTTGATTATCTC+AGG 0.417264 2.2:-59994927 None:intergenic
AAAACTCCGTTTAAGGGATC+CGG 0.419047 2.2:+59995011 MS.gene01953:CDS
AGAACTAGAGATGGAGCATT+GGG 0.430335 2.2:+59994695 None:intergenic
GGCTGACCTACCACGTTAAA+CGG 0.432552 2.2:-59995047 None:intergenic
CGCAAGGCCAAAACCGAGCT+TGG 0.443083 2.2:+59994816 MS.gene01953:CDS
CGCCGGCAGACGATGCAGCC+GGG 0.454146 2.2:-59995283 None:intergenic
TCAACAGCCTTTGGAATCTC+AGG 0.460268 2.2:-59995242 None:intergenic
TGTTGGAACTCACGAATACC+TGG 0.462280 2.2:+59994902 MS.gene01953:CDS
TCGCCGGCAGACGATGCAGC+CGG 0.465871 2.2:-59995284 None:intergenic
TGCATCGTCTGCCGGCGAAA+AGG 0.466384 2.2:+59995289 MS.gene01953:CDS
ACGGCAGTGCAGTTGATTGT+TGG 0.471044 2.2:+59994952 MS.gene01953:CDS
AAAGGGATGTTGTTTAATCT+CGG 0.474175 2.2:-59995177 None:intergenic
TATCAGGGGTTTGCTTGTAA+AGG 0.485912 2.2:+59995118 MS.gene01953:CDS
TCCCCGGCTGCATCGTCTGC+CGG 0.488655 2.2:+59995281 MS.gene01953:CDS
TGTTGTTAGCTTTGCAGCAA+GGG 0.490807 2.2:+59995091 MS.gene01953:CDS
CTTGCTGCAAAGCTAACAAC+AGG 0.490974 2.2:-59995089 None:intergenic
CGGTTCCCGGATCCCTTAAA+CGG 0.499894 2.2:-59995017 None:intergenic
GTTTGAGAAGATATCTTCCC+CGG 0.513450 2.2:+59995265 MS.gene01953:CDS
GACATAGAGCGAGCGTCGGT+GGG 0.522703 2.2:-59994876 None:intergenic
AGACATAGAGCGAGCGTCGG+TGG 0.528304 2.2:-59994877 None:intergenic
TGCAGCAAGGGATCTTATCA+GGG 0.528637 2.2:+59995103 MS.gene01953:CDS
CACAGATTGACTTATAAAAG+AGG 0.540564 2.2:+59995149 MS.gene01953:CDS
ACTGACTTGATTGCCAAGCT+CGG 0.542542 2.2:-59994829 None:intergenic
CTGTTGTTAGCTTTGCAGCA+AGG 0.548555 2.2:+59995090 MS.gene01953:CDS
CCTTGATTATCTCAGGTGCC+AGG 0.550487 2.2:-59994920 None:intergenic
CCTGGCACCTGAGATAATCA+AGG 0.551189 2.2:+59994920 MS.gene01953:CDS
TGGTGTAGCACATCGGATCA+AGG 0.554118 2.2:-59995219 None:intergenic
TAGAACTAGAGATGGAGCAT+TGG 0.559898 2.2:+59994694 None:intergenic
AAACTCCGTTTAAGGGATCC+GGG 0.560479 2.2:+59995012 MS.gene01953:CDS
CCACGTTAAACGGTGTGGCT+CGG 0.563162 2.2:-59995037 None:intergenic
TTATAAGTCAATCTGTGCTG+AGG 0.571793 2.2:-59995143 None:intergenic
GCCGGCAGACGATGCAGCCG+GGG 0.571909 2.2:-59995282 None:intergenic
TCAACAACTCCTAATCAGAA+AGG 0.579889 2.2:+59995329 MS.gene01953:CDS
ATAATCAAGGGTGAAGGTCA+CGG 0.593639 2.2:+59994933 MS.gene01953:CDS
CAAATCAAAGAAAGAACGCA+AGG 0.607512 2.2:+59994800 MS.gene01953:CDS
GATTAGGAGTTGTTGACATG+TGG 0.625031 2.2:-59995322 None:intergenic
ACAGCCTTTGGAATCTCAGG+TGG 0.625096 2.2:-59995239 None:intergenic
GCAGCAAGGGATCTTATCAG+GGG 0.631769 2.2:+59995104 MS.gene01953:CDS
ACCTACCACGTTAAACGGTG+TGG 0.635170 2.2:-59995042 None:intergenic
GATGGCGGCTCAATACATGC+AGG 0.638381 2.2:-59994732 None:intergenic
CCTGAGATAATCAAGGGTGA+AGG 0.640110 2.2:+59994927 MS.gene01953:CDS
ACACGAGGTAGGAGCAACAC+AGG 0.648331 2.2:-59994754 None:intergenic
TCTGCAACGAGCTCAAGTAG+AGG 0.651975 2.2:-59994852 None:intergenic
TGTTGACATGTGGTTAACAA+CGG 0.653551 2.2:-59995312 None:intergenic
GAGGTAGGAGCAACACAGGA+TGG 0.657527 2.2:-59994750 None:intergenic
CTGGCACCTGAGATAATCAA+GGG 0.661653 2.2:+59994921 MS.gene01953:CDS
CCGAGCCACACCGTTTAACG+TGG 0.665030 2.2:+59995037 MS.gene01953:CDS
TCTCAGGTGGTGTAGCACAT+CGG 0.670407 2.2:-59995226 None:intergenic
GTAGGAGCAACACAGGATGG+CGG 0.676336 2.2:-59994747 None:intergenic
TACACCACCTGAGATTCCAA+AGG 0.680244 2.2:+59995235 MS.gene01953:CDS
AAAAGACATAGAGCGAGCGT+CGG 0.699256 2.2:-59994880 None:intergenic
GCCACACCGTTTAACGTGGT+AGG 0.710142 2.2:+59995041 MS.gene01953:CDS
GATTTGCTAGAAAAGAGCCG+AGG 0.729953 2.2:-59994783 None:intergenic
GGTGCTTCTGGAAATCGCAG+CGG 0.758291 2.2:-59995068 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGGGATGTTGTTTAATCT+CGG - chr2.2:59995180-59995199 None:intergenic 30.0%
! CACAGATTGACTTATAAAAG+AGG + chr2.2:59995149-59995168 MS.gene01953:CDS 30.0%
! TTTCTATACGAGTTGTTGTT+TGG + chr2.2:59994987-59995006 MS.gene01953:CDS 30.0%
!!! AAACAACATCCCTTTTTTGA+AGG + chr2.2:59995185-59995204 MS.gene01953:CDS 30.0%
CAAATCAAAGAAAGAACGCA+AGG + chr2.2:59994800-59994819 MS.gene01953:CDS 35.0%
CAATTCACGCCTTCAAAAAA+GGG - chr2.2:59995197-59995216 None:intergenic 35.0%
TCAACAACTCCTAATCAGAA+AGG + chr2.2:59995329-59995348 MS.gene01953:CDS 35.0%
TGTTGACATGTGGTTAACAA+CGG - chr2.2:59995315-59995334 None:intergenic 35.0%
TTATAAGTCAATCTGTGCTG+AGG - chr2.2:59995146-59995165 None:intergenic 35.0%
TTGTCAGAACCTTTCTGATT+AGG - chr2.2:59995341-59995360 None:intergenic 35.0%
TTTGGCAAAACTCCGTTTAA+GGG + chr2.2:59995005-59995024 MS.gene01953:CDS 35.0%
! GAAAGGTTCTGACAATTATC+TGG + chr2.2:59995346-59995365 MS.gene01953:CDS 35.0%
!!! CTTTTTTGAAGGCGTGAATT+GGG + chr2.2:59995196-59995215 MS.gene01953:CDS 35.0%
AAAACTCCGTTTAAGGGATC+CGG + chr2.2:59995011-59995030 MS.gene01953:CDS 40.0%
ATAATCAAGGGTGAAGGTCA+CGG + chr2.2:59994933-59994952 MS.gene01953:CDS 40.0%
CCAATTCACGCCTTCAAAAA+AGG - chr2.2:59995198-59995217 None:intergenic 40.0%
GATTAGGAGTTGTTGACATG+TGG - chr2.2:59995325-59995344 None:intergenic 40.0%
GCAGTTGATTGTTGGACTTA+TGG + chr2.2:59994960-59994979 MS.gene01953:CDS 40.0%
GTTTGAGAAGATATCTTCCC+CGG + chr2.2:59995265-59995284 MS.gene01953:CDS 40.0%
GTTTGGCAAAACTCCGTTTA+AGG + chr2.2:59995004-59995023 MS.gene01953:CDS 40.0%
TTCTCAAACTCAACAGCCTT+TGG - chr2.2:59995254-59995273 None:intergenic 40.0%
! TATCAGGGGTTTGCTTGTAA+AGG + chr2.2:59995118-59995137 MS.gene01953:CDS 40.0%
!! TGTTGTTAGCTTTGCAGCAA+GGG + chr2.2:59995091-59995110 MS.gene01953:CDS 40.0%
!!! CCTTTTTTGAAGGCGTGAAT+TGG + chr2.2:59995195-59995214 MS.gene01953:CDS 40.0%
AAAAGACATAGAGCGAGCGT+CGG - chr2.2:59994883-59994902 None:intergenic 45.0%
AAACTCCGTTTAAGGGATCC+GGG + chr2.2:59995012-59995031 MS.gene01953:CDS 45.0%
AAGCTAACAACAGGTGCTTC+TGG - chr2.2:59995083-59995102 None:intergenic 45.0%
CCTGAGATAATCAAGGGTGA+AGG + chr2.2:59994927-59994946 MS.gene01953:CDS 45.0%
CCTTCACCCTTGATTATCTC+AGG - chr2.2:59994930-59994949 None:intergenic 45.0%
CTGGCACCTGAGATAATCAA+GGG + chr2.2:59994921-59994940 MS.gene01953:CDS 45.0%
CTTGCTGCAAAGCTAACAAC+AGG - chr2.2:59995092-59995111 None:intergenic 45.0%
TACACCACCTGAGATTCCAA+AGG + chr2.2:59995235-59995254 MS.gene01953:CDS 45.0%
TGCAGCAAGGGATCTTATCA+GGG + chr2.2:59995103-59995122 MS.gene01953:CDS 45.0%
TGTTGGAACTCACGAATACC+TGG + chr2.2:59994902-59994921 MS.gene01953:CDS 45.0%
TTGCAGCAAGGGATCTTATC+AGG + chr2.2:59995102-59995121 MS.gene01953:CDS 45.0%
! GCTCGCTCTATGTCTTTTGT+TGG + chr2.2:59994885-59994904 MS.gene01953:CDS 45.0%
! TTGATTGCCAAGCTCGGTTT+TGG - chr2.2:59994826-59994845 None:intergenic 45.0%
!! ACTGACTTGATTGCCAAGCT+CGG - chr2.2:59994832-59994851 None:intergenic 45.0%
!! CTGTTGTTAGCTTTGCAGCA+AGG + chr2.2:59995090-59995109 MS.gene01953:CDS 45.0%
!! GATTTGCTAGAAAAGAGCCG+AGG - chr2.2:59994786-59994805 None:intergenic 45.0%
!! TCAACAGCCTTTGGAATCTC+AGG - chr2.2:59995245-59995264 None:intergenic 45.0%
ACCTACCACGTTAAACGGTG+TGG - chr2.2:59995045-59995064 None:intergenic 50.0%
ACGGCAGTGCAGTTGATTGT+TGG + chr2.2:59994952-59994971 MS.gene01953:CDS 50.0%
CCTGGCACCTGAGATAATCA+AGG + chr2.2:59994920-59994939 MS.gene01953:CDS 50.0%
CCTTGATTATCTCAGGTGCC+AGG - chr2.2:59994923-59994942 None:intergenic 50.0%
GCAGCAAGGGATCTTATCAG+GGG + chr2.2:59995104-59995123 MS.gene01953:CDS 50.0%
GGCTGACCTACCACGTTAAA+CGG - chr2.2:59995050-59995069 None:intergenic 50.0%
TCTCAGGTGGTGTAGCACAT+CGG - chr2.2:59995229-59995248 None:intergenic 50.0%
TCTGCAACGAGCTCAAGTAG+AGG - chr2.2:59994855-59994874 None:intergenic 50.0%
TGGTGTAGCACATCGGATCA+AGG - chr2.2:59995222-59995241 None:intergenic 50.0%
! CTACCTCGTGTTTTTCGCCT+CGG + chr2.2:59994766-59994785 MS.gene01953:CDS 50.0%
! GTTAACAACGGCCTTTTCGC+CGG - chr2.2:59995303-59995322 None:intergenic 50.0%
!! ACAGCCTTTGGAATCTCAGG+TGG - chr2.2:59995242-59995261 None:intergenic 50.0%
ACACGAGGTAGGAGCAACAC+AGG - chr2.2:59994757-59994776 None:intergenic 55.0%
CCACGTTAAACGGTGTGGCT+CGG - chr2.2:59995040-59995059 None:intergenic 55.0%
CGAGGCGAAAAACACGAGGT+AGG - chr2.2:59994768-59994787 None:intergenic 55.0%
CGGTTCCCGGATCCCTTAAA+CGG - chr2.2:59995020-59995039 None:intergenic 55.0%
GAGGTAGGAGCAACACAGGA+TGG - chr2.2:59994753-59994772 None:intergenic 55.0%
GCCACACCGTTTAACGTGGT+AGG + chr2.2:59995041-59995060 MS.gene01953:CDS 55.0%
GTAGGAGCAACACAGGATGG+CGG - chr2.2:59994750-59994769 None:intergenic 55.0%
! GATGGCGGCTCAATACATGC+AGG - chr2.2:59994735-59994754 None:intergenic 55.0%
! GGTGCTTCTGGAAATCGCAG+CGG - chr2.2:59995071-59995090 None:intergenic 55.0%
CCGAGCCACACCGTTTAACG+TGG + chr2.2:59995037-59995056 MS.gene01953:CDS 60.0%
CGCAAGGCCAAAACCGAGCT+TGG + chr2.2:59994816-59994835 MS.gene01953:CDS 60.0%
GAGCCGAGGCGAAAAACACG+AGG - chr2.2:59994772-59994791 None:intergenic 60.0%
TGCATCGTCTGCCGGCGAAA+AGG + chr2.2:59995289-59995308 MS.gene01953:CDS 60.0%
!! AAACGGTGTGGCTCGGTTCC+CGG - chr2.2:59995033-59995052 None:intergenic 60.0%
!! AGACATAGAGCGAGCGTCGG+TGG - chr2.2:59994880-59994899 None:intergenic 60.0%
!! GACATAGAGCGAGCGTCGGT+GGG - chr2.2:59994879-59994898 None:intergenic 60.0%
TCCCCGGCTGCATCGTCTGC+CGG + chr2.2:59995281-59995300 MS.gene01953:CDS 70.0%
TCGCCGGCAGACGATGCAGC+CGG - chr2.2:59995287-59995306 None:intergenic 70.0%
CGCCGGCAGACGATGCAGCC+GGG - chr2.2:59995286-59995305 None:intergenic 75.0%
GCCGGCAGACGATGCAGCCG+GGG - chr2.2:59995285-59995304 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 59994705 59995385 59994705 ID=MS.gene01953
chr2.2 mRNA 59994705 59995385 59994705 ID=MS.gene01953.t1;Parent=MS.gene01953
chr2.2 exon 59994705 59995385 59994705 ID=MS.gene01953.t1.exon1;Parent=MS.gene01953.t1
chr2.2 CDS 59994705 59995385 59994705 ID=cds.MS.gene01953.t1;Parent=MS.gene01953.t1
Gene Sequence

>MS.gene01953

ATGGAGCATTGGGTTTACTGTGCTCAGCCTGCATGTATTGAGCCGCCATCCTGTGTTGCTCCTACCTCGTGTTTTTCGCCTCGGCTCTTTTCTAGCAAATCAAAGAAAGAACGCAAGGCCAAAACCGAGCTTGGCAATCAAGTCAGTCCTCTACTTGAGCTCGTTGCAGAGCCCACCGACGCTCGCTCTATGTCTTTTGTTGGAACTCACGAATACCTGGCACCTGAGATAATCAAGGGTGAAGGTCACGGCAGTGCAGTTGATTGTTGGACTTATGGAATCTTTCTATACGAGTTGTTGTTTGGCAAAACTCCGTTTAAGGGATCCGGGAACCGAGCCACACCGTTTAACGTGGTAGGTCAGCCGCTGCGATTTCCAGAAGCACCTGTTGTTAGCTTTGCAGCAAGGGATCTTATCAGGGGTTTGCTTGTAAAGGAACCTCAGCACAGATTGACTTATAAAAGAGGTGCAACCGAGATTAAACAACATCCCTTTTTTGAAGGCGTGAATTGGGCCTTGATCCGATGTGCTACACCACCTGAGATTCCAAAGGCTGTTGAGTTTGAGAAGATATCTTCCCCGGCTGCATCGTCTGCCGGCGAAAAGGCCGTTGTTAACCACATGTCAACAACTCCTAATCAGAAAGGTTCTGACAATTATCTGGAGTTTGATTTCTTTTAG
Protein sequence

>MS.gene01953.t1

MEHWVYCAQPACIEPPSCVAPTSCFSPRLFSSKSKKERKAKTELGNQVSPLLELVAEPTDARSMSFVGTHEYLAPEIIKGEGHGSAVDCWTYGIFLYELLFGKTPFKGSGNRATPFNVVGQPLRFPEAPVVSFAARDLIRGLLVKEPQHRLTYKRGATEIKQHPFFEGVNWALIRCATPPEIPKAVEFEKISSPAASSAGEKAVVNHMSTTPNQKGSDNYLEFDFF