Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019538.t1 | RHN81411.1 | 75.3 | 733 | 180 | 1 | 25 | 756 | 18 | 750 | 0.00E+00 | 1143.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019538.t1 | Q9SY66 | 45.6 | 676 | 351 | 6 | 18 | 689 | 17 | 679 | 2.0e-170 | 600.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019538.t1 | A0A396JX52 | 75.3 | 733 | 180 | 1 | 25 | 756 | 18 | 750 | 0.0e+00 | 1143.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene019538.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019538.t1 | MTR_3g450850 | 67.658 | 269 | 86 | 1 | 25 | 292 | 18 | 286 | 4.46e-125 | 377 |
| MS.gene019538.t1 | MTR_4g071400 | 80.165 | 121 | 24 | 0 | 597 | 717 | 1 | 121 | 9.35e-63 | 206 |
| MS.gene019538.t1 | MTR_3g012160 | 94.624 | 93 | 5 | 0 | 201 | 293 | 1 | 93 | 2.87e-55 | 184 |
| MS.gene019538.t1 | MTR_4g035820 | 39.068 | 279 | 160 | 3 | 32 | 308 | 54 | 324 | 5.70e-53 | 195 |
| MS.gene019538.t1 | MTR_4g035820 | 48.958 | 96 | 49 | 0 | 441 | 536 | 326 | 421 | 1.14e-20 | 97.4 |
| MS.gene019538.t1 | MTR_1g070355 | 30.033 | 303 | 189 | 8 | 7 | 298 | 19 | 309 | 1.85e-29 | 124 |
| MS.gene019538.t1 | MTR_6g051425 | 29.371 | 286 | 184 | 8 | 54 | 333 | 18 | 291 | 1.48e-27 | 115 |
| MS.gene019538.t1 | MTR_3g095940 | 88.889 | 54 | 6 | 0 | 506 | 559 | 90 | 143 | 8.20e-26 | 104 |
| MS.gene019538.t1 | MTR_6g014355 | 42.478 | 113 | 63 | 1 | 134 | 244 | 14 | 126 | 3.07e-18 | 85.5 |
| MS.gene019538.t1 | MTR_5g076070 | 26.271 | 236 | 160 | 6 | 59 | 292 | 59 | 282 | 3.77e-18 | 85.9 |
| MS.gene019538.t1 | MTR_5g083630 | 65.517 | 58 | 20 | 0 | 202 | 259 | 19 | 76 | 2.35e-17 | 79.0 |
| MS.gene019538.t1 | MTR_0558s0020 | 24.818 | 274 | 182 | 6 | 46 | 304 | 32 | 296 | 7.58e-16 | 79.3 |
| MS.gene019538.t1 | MTR_8g446020 | 30.872 | 149 | 95 | 3 | 160 | 304 | 112 | 256 | 3.54e-12 | 67.8 |
| MS.gene019538.t1 | MTR_7g446520 | 23.904 | 251 | 155 | 7 | 59 | 304 | 94 | 313 | 4.88e-12 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019538.t1 | AT1G10240 | 45.562 | 676 | 351 | 6 | 18 | 689 | 17 | 679 | 0.0 | 619 |
| MS.gene019538.t1 | AT1G10240 | 45.562 | 676 | 351 | 6 | 18 | 689 | 17 | 679 | 0.0 | 619 |
| MS.gene019538.t1 | AT5G28530 | 42.178 | 652 | 353 | 6 | 30 | 670 | 32 | 670 | 1.43e-178 | 527 |
| MS.gene019538.t1 | AT5G28530 | 42.178 | 652 | 353 | 6 | 30 | 670 | 32 | 670 | 1.43e-178 | 527 |
| MS.gene019538.t1 | AT5G28530 | 42.025 | 652 | 358 | 6 | 30 | 670 | 32 | 674 | 8.85e-178 | 525 |
| MS.gene019538.t1 | AT5G28530 | 37.346 | 648 | 327 | 7 | 30 | 670 | 32 | 607 | 1.59e-143 | 435 |
| MS.gene019538.t1 | AT5G28530 | 37.191 | 648 | 332 | 7 | 30 | 670 | 32 | 611 | 1.20e-142 | 433 |
| MS.gene019538.t1 | AT4G38180 | 31.379 | 580 | 372 | 9 | 53 | 620 | 73 | 638 | 4.85e-84 | 283 |
| MS.gene019538.t1 | AT4G15090 | 27.364 | 698 | 464 | 14 | 47 | 719 | 36 | 715 | 1.05e-75 | 261 |
| MS.gene019538.t1 | AT4G15090 | 27.364 | 698 | 464 | 14 | 47 | 719 | 36 | 715 | 1.05e-75 | 261 |
| MS.gene019538.t1 | AT4G15090 | 27.364 | 698 | 464 | 14 | 47 | 719 | 36 | 715 | 1.05e-75 | 261 |
| MS.gene019538.t1 | AT4G15090 | 27.364 | 698 | 464 | 14 | 47 | 719 | 36 | 715 | 1.05e-75 | 261 |
| MS.gene019538.t1 | AT4G15090 | 27.364 | 698 | 464 | 14 | 47 | 719 | 36 | 715 | 1.05e-75 | 261 |
| MS.gene019538.t1 | AT3G22170 | 27.179 | 585 | 369 | 14 | 53 | 617 | 70 | 617 | 4.60e-67 | 238 |
| MS.gene019538.t1 | AT3G22170 | 27.179 | 585 | 369 | 14 | 53 | 617 | 70 | 617 | 4.60e-67 | 238 |
| MS.gene019538.t1 | AT1G76320 | 27.113 | 568 | 370 | 8 | 59 | 613 | 3 | 539 | 1.33e-66 | 233 |
| MS.gene019538.t1 | AT1G76320 | 27.113 | 568 | 370 | 8 | 59 | 613 | 3 | 539 | 2.24e-66 | 234 |
| MS.gene019538.t1 | AT1G76320 | 27.113 | 568 | 370 | 8 | 59 | 613 | 3 | 539 | 2.24e-66 | 234 |
| MS.gene019538.t1 | AT1G76320 | 27.113 | 568 | 370 | 8 | 59 | 613 | 3 | 539 | 2.26e-66 | 234 |
| MS.gene019538.t1 | AT2G27110 | 26.634 | 612 | 390 | 17 | 53 | 653 | 49 | 612 | 7.47e-56 | 206 |
| MS.gene019538.t1 | AT2G27110 | 26.634 | 612 | 390 | 17 | 53 | 653 | 49 | 612 | 7.47e-56 | 206 |
| MS.gene019538.t1 | AT3G06250 | 26.261 | 575 | 345 | 15 | 53 | 613 | 190 | 699 | 6.85e-53 | 196 |
| MS.gene019538.t1 | AT3G06250 | 26.261 | 575 | 345 | 15 | 53 | 613 | 190 | 699 | 6.85e-53 | 196 |
| MS.gene019538.t1 | AT3G06250 | 26.261 | 575 | 345 | 15 | 53 | 613 | 190 | 699 | 6.85e-53 | 196 |
| MS.gene019538.t1 | AT2G27110 | 28.802 | 434 | 291 | 10 | 228 | 653 | 44 | 467 | 6.05e-51 | 190 |
| MS.gene019538.t1 | AT5G18960 | 25.561 | 579 | 357 | 17 | 48 | 613 | 206 | 723 | 1.43e-48 | 184 |
| MS.gene019538.t1 | AT5G18960 | 25.561 | 579 | 357 | 17 | 48 | 613 | 206 | 723 | 1.43e-48 | 184 |
| MS.gene019538.t1 | AT4G19990 | 26.446 | 605 | 374 | 15 | 55 | 638 | 23 | 577 | 3.13e-48 | 182 |
| MS.gene019538.t1 | AT4G19990 | 26.446 | 605 | 374 | 15 | 55 | 638 | 23 | 577 | 3.28e-48 | 182 |
| MS.gene019538.t1 | AT4G19990 | 26.446 | 605 | 374 | 15 | 55 | 638 | 23 | 577 | 3.28e-48 | 182 |
| MS.gene019538.t1 | AT4G19990 | 26.446 | 605 | 374 | 15 | 55 | 638 | 23 | 577 | 3.85e-48 | 182 |
| MS.gene019538.t1 | AT4G19990 | 26.446 | 605 | 374 | 15 | 55 | 638 | 23 | 577 | 3.85e-48 | 182 |
| MS.gene019538.t1 | AT4G19990 | 26.446 | 605 | 374 | 15 | 55 | 638 | 37 | 591 | 4.64e-48 | 182 |
| MS.gene019538.t1 | AT2G32250 | 23.902 | 569 | 371 | 11 | 56 | 618 | 41 | 553 | 1.44e-47 | 181 |
| MS.gene019538.t1 | AT2G32250 | 23.902 | 569 | 371 | 11 | 56 | 618 | 41 | 553 | 2.32e-47 | 181 |
| MS.gene019538.t1 | AT2G32250 | 23.902 | 569 | 371 | 11 | 56 | 618 | 41 | 553 | 2.32e-47 | 181 |
| MS.gene019538.t1 | AT2G32250 | 23.902 | 569 | 371 | 11 | 56 | 618 | 41 | 553 | 2.46e-47 | 181 |
| MS.gene019538.t1 | AT2G32250 | 23.902 | 569 | 371 | 11 | 56 | 618 | 41 | 553 | 2.46e-47 | 181 |
| MS.gene019538.t1 | AT2G32250 | 23.902 | 569 | 371 | 11 | 56 | 618 | 41 | 553 | 2.46e-47 | 181 |
| MS.gene019538.t1 | AT2G32250 | 23.852 | 566 | 369 | 11 | 59 | 618 | 3 | 512 | 6.34e-47 | 179 |
| MS.gene019538.t1 | AT2G32250 | 23.852 | 566 | 369 | 11 | 59 | 618 | 3 | 512 | 6.60e-47 | 179 |
| MS.gene019538.t1 | AT4G38170 | 29.091 | 385 | 262 | 7 | 241 | 619 | 21 | 400 | 4.19e-46 | 173 |
| MS.gene019538.t1 | AT1G80010 | 24.507 | 608 | 372 | 19 | 53 | 616 | 95 | 659 | 5.82e-46 | 176 |
| MS.gene019538.t1 | AT4G19990 | 27.158 | 556 | 340 | 13 | 104 | 638 | 9 | 520 | 3.62e-45 | 173 |
| MS.gene019538.t1 | AT1G52520 | 24.486 | 584 | 391 | 17 | 52 | 616 | 84 | 636 | 2.19e-44 | 171 |
Find 102 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCACATATTCTTGAGTTTC+TGG | 0.087082 | 8.1:+70414606 | None:intergenic |
| GCAATCCAAACTTACTTATT+AGG | 0.125684 | 8.1:+70414363 | None:intergenic |
| ACATCACAAACATCGTTATT+TGG | 0.152623 | 8.1:+70414564 | None:intergenic |
| ACCCATCTGGTCTTTATAAA+AGG | 0.174050 | 8.1:-70416521 | MS.gene019538:CDS |
| ATGATATGCCACTTGGAATA+TGG | 0.191467 | 8.1:-70415912 | MS.gene019538:CDS |
| TTCCTTTGCGTGGGCTTTAA+AGG | 0.228847 | 8.1:-70415821 | MS.gene019538:intron |
| CAATCCAAACTTACTTATTA+GGG | 0.237365 | 8.1:+70414364 | None:intergenic |
| ATTCGAGCATACAAATCTTT+TGG | 0.247827 | 8.1:-70415976 | MS.gene019538:CDS |
| CTCATGTTGGAGCAACTTAA+AGG | 0.285515 | 8.1:+70415040 | None:intergenic |
| TCATCACAATCATGAGTTAT+TGG | 0.289585 | 8.1:-70416334 | MS.gene019538:CDS |
| GTTGACTACATGGCTAGATT+TGG | 0.300626 | 8.1:+70414761 | None:intergenic |
| TATTGATGAATGTAACAAAT+TGG | 0.311506 | 8.1:-70416028 | MS.gene019538:CDS |
| GCAGCTTCAACTCCTCCTTT+TGG | 0.319527 | 8.1:+70414492 | None:intergenic |
| TTGACTACATGGCTAGATTT+GGG | 0.326040 | 8.1:+70414762 | None:intergenic |
| TCAATCTCTGATTCGATGTT+TGG | 0.328733 | 8.1:-70414712 | MS.gene019538:CDS |
| TCAAGAATATGTGATTGATT+TGG | 0.332734 | 8.1:-70414598 | MS.gene019538:CDS |
| TGATCCTCTTGGCCGACCTT+TGG | 0.339991 | 8.1:+70414518 | None:intergenic |
| TTGGTGTTGCTGTCAAAATT+AGG | 0.355142 | 8.1:-70415169 | MS.gene019538:CDS |
| AAAGTCATTCAAGATATAAA+AGG | 0.358979 | 8.1:-70414633 | MS.gene019538:CDS |
| TTTGCTGGGATGACAACAAT+AGG | 0.362753 | 8.1:-70415360 | MS.gene019538:CDS |
| AAAATCTGTCAAACTTGTAT+TGG | 0.376571 | 8.1:+70415293 | None:intergenic |
| ATTCAATCCCAGAGTTGTAT+TGG | 0.389914 | 8.1:-70416132 | MS.gene019538:CDS |
| CAAAATGCATGCTTTGTGTT+TGG | 0.392144 | 8.1:+70415615 | None:intergenic |
| AGATCAAGATCCCGCACTTA+AGG | 0.392664 | 8.1:-70415658 | MS.gene019538:CDS |
| AATAGTGAATTCATATTTGA+AGG | 0.396961 | 8.1:+70416047 | None:intergenic |
| GATGCAGCATGTTCCTCAAT+AGG | 0.398909 | 8.1:+70415080 | None:intergenic |
| CTAAATATTTGCCCTTCAAA+AGG | 0.402295 | 8.1:+70416651 | None:intergenic |
| CGAATATCTCTTTCTAGAAA+AGG | 0.402638 | 8.1:+70416162 | None:intergenic |
| TGGTAGTGATTGGCAATCTA+AGG | 0.404374 | 8.1:-70416787 | MS.gene019538:CDS |
| TTTCTTTACCAATACAACTC+TGG | 0.409477 | 8.1:+70416124 | None:intergenic |
| TGGAAAAGAGCAAGTCAATA+TGG | 0.415923 | 8.1:+70415424 | None:intergenic |
| GAGCATATTATGTGGGCATT+TGG | 0.419517 | 8.1:-70416006 | MS.gene019538:CDS |
| TGATATGCCACTTGGAATAT+GGG | 0.434301 | 8.1:-70415911 | MS.gene019538:CDS |
| TTCTTTACCAATACAACTCT+GGG | 0.436134 | 8.1:+70416125 | None:intergenic |
| AACCAGCTTTCGACAACAAT+AGG | 0.437905 | 8.1:+70416247 | None:intergenic |
| CTTTCTTGTCTCCATCGAAA+AGG | 0.441125 | 8.1:+70414795 | None:intergenic |
| CCCATCTGGTCTTTATAAAA+GGG | 0.446061 | 8.1:-70416520 | MS.gene019538:CDS |
| TTGGGCATTAGCTTATTTGA+AGG | 0.451954 | 8.1:-70415391 | MS.gene019538:CDS |
| ATCCTATTGTTGTCGAAAGC+TGG | 0.455314 | 8.1:-70416249 | MS.gene019538:CDS |
| ACATGCTATTCGTGTGCTAA+TGG | 0.455837 | 8.1:-70414853 | MS.gene019538:CDS |
| AAAATAATGATGGTAGTGAT+TGG | 0.465147 | 8.1:-70416797 | MS.gene019538:CDS |
| CGAAAGAGAGTGGAAATCAA+TGG | 0.466880 | 8.1:-70415475 | MS.gene019538:CDS |
| CCTCATGTCAGTAACATTGA+TGG | 0.471488 | 8.1:-70416681 | MS.gene019538:CDS |
| GGGCAAATATTTAGTAATGA+TGG | 0.472481 | 8.1:-70416642 | MS.gene019538:CDS |
| TTGCTGGGATGACAACAATA+GGG | 0.477276 | 8.1:-70415359 | MS.gene019538:CDS |
| GGAAAACATGTACTGATATG+AGG | 0.482616 | 8.1:+70415101 | None:intergenic |
| TTTCTTGTCTCCATCGAAAA+GGG | 0.485154 | 8.1:+70414796 | None:intergenic |
| GTTGGAATGTTGCGATAAGC+AGG | 0.492886 | 8.1:+70416288 | None:intergenic |
| TGATTAGTTCCACCACAACT+TGG | 0.507884 | 8.1:+70414444 | None:intergenic |
| CGCTGTCATGTTCCAAGTTG+TGG | 0.511566 | 8.1:-70414456 | MS.gene019538:CDS |
| CCAAGAGGATCAAGACCAAA+AGG | 0.514192 | 8.1:-70414507 | MS.gene019538:CDS |
| TGGAGCAACTTAAAGGCATA+TGG | 0.514224 | 8.1:+70415047 | None:intergenic |
| GTCAAAATTAGGAATCAAGC+TGG | 0.516805 | 8.1:-70415158 | MS.gene019538:CDS |
| TAGATAACTCAATCTCATGT+TGG | 0.522807 | 8.1:+70415027 | None:intergenic |
| ACGGTGGGCGTCAAGTAAGT+TGG | 0.528714 | 8.1:-70414941 | MS.gene019538:CDS |
| TAGCATTGCAGTTGACTACA+TGG | 0.529793 | 8.1:+70414751 | None:intergenic |
| TTGTGGCAAAGTTGTCAAGT+TGG | 0.535071 | 8.1:-70415581 | MS.gene019538:CDS |
| ACAAATTGGAGCATATTATG+TGG | 0.539309 | 8.1:-70416014 | MS.gene019538:CDS |
| AGAAGATTTCTTCCTTTGCG+TGG | 0.551128 | 8.1:-70415831 | MS.gene019538:CDS |
| GAAGATTTCTTCCTTTGCGT+GGG | 0.554338 | 8.1:-70415830 | MS.gene019538:CDS |
| ATACGACTTTGATCAGCAGT+TGG | 0.558928 | 8.1:+70416270 | None:intergenic |
| AATCGTTATGATATGCCACT+TGG | 0.563602 | 8.1:-70415919 | MS.gene019538:CDS |
| TGTCAGTAACATTGATGGGT+GGG | 0.565774 | 8.1:-70416676 | MS.gene019538:CDS |
| TTAATGGCTTCCTTAAGTGC+GGG | 0.567307 | 8.1:+70415648 | None:intergenic |
| TTTAATGGCTTCCTTAAGTG+CGG | 0.570999 | 8.1:+70415647 | None:intergenic |
| AGGGAATTTGTTTGTCATCG+TGG | 0.571012 | 8.1:-70416501 | MS.gene019538:CDS |
| CAACATCATACAAAAGTTGA+CGG | 0.577068 | 8.1:-70414960 | MS.gene019538:CDS |
| TAATGCCCAAAATTCACGAT+TGG | 0.579684 | 8.1:+70415404 | None:intergenic |
| TAGGAATCAAGCTGGAGAAG+AGG | 0.579829 | 8.1:-70415150 | MS.gene019538:CDS |
| TTGTTACAAAATCCAAAGGT+CGG | 0.582656 | 8.1:-70414530 | MS.gene019538:CDS |
| CGGATGATTTCGAAAGAGAG+TGG | 0.582752 | 8.1:-70415485 | MS.gene019538:CDS |
| ATCATACAAAAGTTGACGGT+GGG | 0.583166 | 8.1:-70414956 | MS.gene019538:CDS |
| ACAACGCAACATTAACGAAG+TGG | 0.585119 | 8.1:-70416466 | MS.gene019538:CDS |
| CCCATTGTTACAAAATCCAA+AGG | 0.587534 | 8.1:-70414534 | MS.gene019538:CDS |
| AAATCTTCAAAATGTAATTG+TGG | 0.587616 | 8.1:-70416426 | MS.gene019538:CDS |
| CAAATTGGAGCATATTATGT+GGG | 0.593912 | 8.1:-70416013 | MS.gene019538:CDS |
| CTCATGTCAGTAACATTGAT+GGG | 0.595497 | 8.1:-70416680 | MS.gene019538:CDS |
| AATCCAAACTTACTTATTAG+GGG | 0.596179 | 8.1:+70414365 | None:intergenic |
| TAATTGTGGTGCAAAGATGT+TGG | 0.600117 | 8.1:-70416412 | MS.gene019538:CDS |
| ATGTCAGTAACATTGATGGG+TGG | 0.603297 | 8.1:-70416677 | MS.gene019538:CDS |
| CAGAGATTGAAACTTAGTAG+AGG | 0.604303 | 8.1:+70414725 | None:intergenic |
| TGACTACATGGCTAGATTTG+GGG | 0.607240 | 8.1:+70414763 | None:intergenic |
| TGGGTTGGTGTTGATAATCA+CGG | 0.609843 | 8.1:-70415892 | MS.gene019538:CDS |
| ATGTCTGAAGAAAATAATGA+TGG | 0.610520 | 8.1:-70416807 | MS.gene019538:CDS |
| TACTTATTAGGGGATTGAGA+AGG | 0.610633 | 8.1:+70414375 | None:intergenic |
| TGCACAATTTGATTTGTGCA+AGG | 0.611924 | 8.1:-70415451 | MS.gene019538:CDS |
| TACCTTTAAAGCCCACGCAA+AGG | 0.613106 | 8.1:+70415819 | None:intergenic |
| GGTGTATCATTTAGAATGTG+CGG | 0.618416 | 8.1:-70415505 | MS.gene019538:CDS |
| CTTTAAACAAGAGTTTCACA+AGG | 0.629391 | 8.1:-70415526 | MS.gene019538:CDS |
| GAATTTGTTTGTCATCGTGG+TGG | 0.635704 | 8.1:-70416498 | MS.gene019538:CDS |
| AAATCCAAAGGTCGGCCAAG+AGG | 0.639624 | 8.1:-70414522 | MS.gene019538:CDS |
| AGAGAACGAGTTGCTACTAG+AGG | 0.645909 | 8.1:-70414663 | MS.gene019538:CDS |
| ATGCCACTTGGAATATGGGT+TGG | 0.648688 | 8.1:-70415907 | MS.gene019538:CDS |
| CCATCAATGTTACTGACATG+AGG | 0.654897 | 8.1:+70416681 | None:intergenic |
| GAGGAGTTGAAGCTGCAAAG+AGG | 0.660327 | 8.1:-70414485 | MS.gene019538:CDS |
| GCAGCAACTGAAATTGACAA+TGG | 0.666988 | 8.1:-70414996 | MS.gene019538:CDS |
| AGAGGATCAAGACCAAAAGG+AGG | 0.673931 | 8.1:-70414504 | MS.gene019538:CDS |
| CATCATACAAAAGTTGACGG+TGG | 0.678338 | 8.1:-70414957 | MS.gene019538:CDS |
| ACACCAACCCATATTCCAAG+TGG | 0.679959 | 8.1:+70415904 | None:intergenic |
| TGTCATGTTCCAAGTTGTGG+TGG | 0.684261 | 8.1:-70414453 | MS.gene019538:CDS |
| ATAACGATTTATTCTATACG+TGG | 0.695494 | 8.1:+70415933 | None:intergenic |
| CTTGGAACATGACAGCGACG+AGG | 0.719993 | 8.1:+70414462 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCATTATCTTATAATTGA+GGG | - | chr8.1:70415965-70415984 | MS.gene019538:CDS | 15.0% |
| !! | TAATTATTATATCTTTGTGT+AGG | - | chr8.1:70415989-70416008 | MS.gene019538:CDS | 15.0% |
| !!! | TCAATTTTTTTCTTTTTGTT+TGG | + | chr8.1:70416779-70416798 | None:intergenic | 15.0% |
| !! | AAAGTCATTCAAGATATAAA+AGG | - | chr8.1:70416548-70416567 | MS.gene019538:CDS | 20.0% |
| !! | AAATCTTCAAAATGTAATTG+TGG | - | chr8.1:70414755-70414774 | MS.gene019538:CDS | 20.0% |
| !! | AATAGTGAATTCATATTTGA+AGG | + | chr8.1:70415137-70415156 | None:intergenic | 20.0% |
| !! | GAATCATTATCTTATAATTG+AGG | - | chr8.1:70415964-70415983 | MS.gene019538:CDS | 20.0% |
| !! | GCATATATAAAAAGATTCTT+AGG | - | chr8.1:70415863-70415882 | MS.gene019538:CDS | 20.0% |
| !! | TATTGATGAATGTAACAAAT+TGG | - | chr8.1:70415153-70415172 | MS.gene019538:CDS | 20.0% |
| !! | TTAGAGAAAAAAATAGTTAC+AGG | - | chr8.1:70414989-70415008 | MS.gene019538:CDS | 20.0% |
| ! | AAAATCTGTCAAACTTGTAT+TGG | + | chr8.1:70415891-70415910 | None:intergenic | 25.0% |
| ! | ATAACGATTTATTCTATACG+TGG | + | chr8.1:70415251-70415270 | None:intergenic | 25.0% |
| ! | ATGTCTGAAGAAAATAATGA+TGG | - | chr8.1:70414374-70414393 | MS.gene019538:CDS | 25.0% |
| ! | TATTATATCTTTGTGTAGGT+TGG | - | chr8.1:70415993-70416012 | MS.gene019538:CDS | 25.0% |
| ! | TCAAGAATATGTGATTGATT+TGG | - | chr8.1:70416583-70416602 | MS.gene019538:CDS | 25.0% |
| ! | TTGGTTGCTATAAAAACAAT+TGG | - | chr8.1:70414788-70414807 | MS.gene019538:CDS | 25.0% |
| !! | AAAATAATGATGGTAGTGAT+TGG | - | chr8.1:70414384-70414403 | MS.gene019538:CDS | 25.0% |
| !! | GTTTGGCAATTTCATTTTAA+TGG | + | chr8.1:70415552-70415571 | None:intergenic | 25.0% |
| !! | TCTTTATTTGCACGAAAAAA+TGG | - | chr8.1:70414581-70414600 | MS.gene019538:CDS | 25.0% |
| !! | TGATCTGTAAGAATAGTTTT+TGG | + | chr8.1:70415509-70415528 | None:intergenic | 25.0% |
| !!! | TAGTTTTTGGATATTTTCCT+TGG | + | chr8.1:70415496-70415515 | None:intergenic | 25.0% |
| !!! | TATAAAAACAATTGGCTTTG+AGG | - | chr8.1:70414796-70414815 | MS.gene019538:CDS | 25.0% |
| !!! | TGTAAAGAATTTGAGTTTTC+TGG | - | chr8.1:70416293-70416312 | MS.gene019538:CDS | 25.0% |
| ACAAATTGGAGCATATTATG+TGG | - | chr8.1:70415167-70415186 | MS.gene019538:CDS | 30.0% | |
| ACATCACAAACATCGTTATT+TGG | + | chr8.1:70416620-70416639 | None:intergenic | 30.0% | |
| ATCACATATTCTTGAGTTTC+TGG | + | chr8.1:70416578-70416597 | None:intergenic | 30.0% | |
| CAAATTGGAGCATATTATGT+GGG | - | chr8.1:70415168-70415187 | MS.gene019538:CDS | 30.0% | |
| CAACATCATACAAAAGTTGA+CGG | - | chr8.1:70416221-70416240 | MS.gene019538:CDS | 30.0% | |
| CTAAATATTTGCCCTTCAAA+AGG | + | chr8.1:70414533-70414552 | None:intergenic | 30.0% | |
| CTTTAAACAAGAGTTTCACA+AGG | - | chr8.1:70415655-70415674 | MS.gene019538:CDS | 30.0% | |
| GGGCAAATATTTAGTAATGA+TGG | - | chr8.1:70414539-70414558 | MS.gene019538:CDS | 30.0% | |
| TAGATAACTCAATCTCATGT+TGG | + | chr8.1:70416157-70416176 | None:intergenic | 30.0% | |
| TCATCACAATCATGAGTTAT+TGG | - | chr8.1:70414847-70414866 | MS.gene019538:CDS | 30.0% | |
| TGAGTTATTGGATGAAAAAG+AGG | - | chr8.1:70414859-70414878 | MS.gene019538:CDS | 30.0% | |
| TTCTTTACCAATACAACTCT+GGG | + | chr8.1:70415059-70415078 | None:intergenic | 30.0% | |
| TTGTTACAAAATCCAAAGGT+CGG | - | chr8.1:70416651-70416670 | MS.gene019538:CDS | 30.0% | |
| TTTCTTTACCAATACAACTC+TGG | + | chr8.1:70415060-70415079 | None:intergenic | 30.0% | |
| ! | ACCTTTGGATTTTGTAACAA+TGG | + | chr8.1:70416651-70416670 | None:intergenic | 30.0% |
| ! | ATTCGAGCATACAAATCTTT+TGG | - | chr8.1:70415205-70415224 | MS.gene019538:intron | 30.0% |
| ! | ATTTTGCATATGGCATATTG+TGG | - | chr8.1:70415583-70415602 | MS.gene019538:CDS | 30.0% |
| ! | CCTTTGGATTTTGTAACAAT+GGG | + | chr8.1:70416650-70416669 | None:intergenic | 30.0% |
| ! | CGAATATCTCTTTCTAGAAA+AGG | + | chr8.1:70415022-70415041 | None:intergenic | 30.0% |
| ! | CTTTTCCAATCGTGAATTTT+GGG | - | chr8.1:70415772-70415791 | MS.gene019538:intron | 30.0% |
| ! | TCTTTTCCAATCGTGAATTT+TGG | - | chr8.1:70415771-70415790 | MS.gene019538:intron | 30.0% |
| !! | AATTGGCTTTGAGGAAAAAT+GGG | - | chr8.1:70414805-70414824 | MS.gene019538:CDS | 30.0% |
| !! | GAAAAAATGGATTTTCGATC+AGG | - | chr8.1:70414594-70414613 | MS.gene019538:CDS | 30.0% |
| !! | GAAAAGGGAGATATTTTGAA+GGG | + | chr8.1:70416373-70416392 | None:intergenic | 30.0% |
| !!! | AAGTTGGTTTTCTTTTGCAT+TGG | - | chr8.1:70415616-70415635 | MS.gene019538:CDS | 30.0% |
| !!! | AGTTGGTTTTCTTTTGCATT+GGG | - | chr8.1:70415617-70415636 | MS.gene019538:CDS | 30.0% |
| AATCGTTATGATATGCCACT+TGG | - | chr8.1:70415262-70415281 | MS.gene019538:intron | 35.0% | |
| ATCATACAAAAGTTGACGGT+GGG | - | chr8.1:70416225-70416244 | MS.gene019538:CDS | 35.0% | |
| ATTCAATCCCAGAGTTGTAT+TGG | - | chr8.1:70415049-70415068 | MS.gene019538:CDS | 35.0% | |
| CAAAATGCATGCTTTGTGTT+TGG | + | chr8.1:70415569-70415588 | None:intergenic | 35.0% | |
| CAGAGATTGAAACTTAGTAG+AGG | + | chr8.1:70416459-70416478 | None:intergenic | 35.0% | |
| CCCATTGTTACAAAATCCAA+AGG | - | chr8.1:70416647-70416666 | MS.gene019538:CDS | 35.0% | |
| CTCATGTCAGTAACATTGAT+GGG | - | chr8.1:70414501-70414520 | MS.gene019538:CDS | 35.0% | |
| GTCAAAATTAGGAATCAAGC+TGG | - | chr8.1:70416023-70416042 | MS.gene019538:CDS | 35.0% | |
| TAATGCCCAAAATTCACGAT+TGG | + | chr8.1:70415780-70415799 | None:intergenic | 35.0% | |
| TAATTGTGGTGCAAAGATGT+TGG | - | chr8.1:70414769-70414788 | MS.gene019538:CDS | 35.0% | |
| TACTTATTAGGGGATTGAGA+AGG | + | chr8.1:70416809-70416828 | None:intergenic | 35.0% | |
| TGGAAAAGAGCAAGTCAATA+TGG | + | chr8.1:70415760-70415779 | None:intergenic | 35.0% | |
| TTGACTACATGGCTAGATTT+GGG | + | chr8.1:70416422-70416441 | None:intergenic | 35.0% | |
| TTTAATGGCTTCCTTAAGTG+CGG | + | chr8.1:70415537-70415556 | None:intergenic | 35.0% | |
| TTTCTTGTCTCCATCGAAAA+GGG | + | chr8.1:70416388-70416407 | None:intergenic | 35.0% | |
| ! | ACCCATCTGGTCTTTATAAA+AGG | - | chr8.1:70414660-70414679 | MS.gene019538:CDS | 35.0% |
| ! | ATGATATGCCACTTGGAATA+TGG | - | chr8.1:70415269-70415288 | MS.gene019538:intron | 35.0% |
| ! | ATTTTCCTTGGACAAAAGCT+AGG | + | chr8.1:70415484-70415503 | None:intergenic | 35.0% |
| ! | CAAAATATCTCCCTTTTCGA+TGG | - | chr8.1:70416375-70416394 | MS.gene019538:CDS | 35.0% |
| ! | CAAAGCATGCATTTTGCATA+TGG | - | chr8.1:70415573-70415592 | MS.gene019538:CDS | 35.0% |
| ! | CACGGAAATTCGATTTTCTT+TGG | - | chr8.1:70415307-70415326 | MS.gene019538:CDS | 35.0% |
| ! | CAGTACATGTTTTCCTATTG+AGG | - | chr8.1:70416088-70416107 | MS.gene019538:CDS | 35.0% |
| ! | CCCATCTGGTCTTTATAAAA+GGG | - | chr8.1:70414661-70414680 | MS.gene019538:CDS | 35.0% |
| ! | CCCTTTTATAAAGACCAGAT+GGG | + | chr8.1:70414664-70414683 | None:intergenic | 35.0% |
| ! | GGAAAACATGTACTGATATG+AGG | + | chr8.1:70416083-70416102 | None:intergenic | 35.0% |
| ! | GGTGTATCATTTAGAATGTG+CGG | - | chr8.1:70415676-70415695 | MS.gene019538:CDS | 35.0% |
| ! | TCAATCTCTGATTCGATGTT+TGG | - | chr8.1:70416469-70416488 | MS.gene019538:CDS | 35.0% |
| ! | TCCCTTTTATAAAGACCAGA+TGG | + | chr8.1:70414665-70414684 | None:intergenic | 35.0% |
| ! | TGAGGCAAATTTTCGTTTTG+TGG | + | chr8.1:70414485-70414504 | None:intergenic | 35.0% |
| ! | TGATATGCCACTTGGAATAT+GGG | - | chr8.1:70415270-70415289 | MS.gene019538:intron | 35.0% |
| ! | TTGGGCATTAGCTTATTTGA+AGG | - | chr8.1:70415790-70415809 | MS.gene019538:intron | 35.0% |
| !! | CAATTGGCTTTGAGGAAAAA+TGG | - | chr8.1:70414804-70414823 | MS.gene019538:CDS | 35.0% |
| !! | CGAAAAGGGAGATATTTTGA+AGG | + | chr8.1:70416374-70416393 | None:intergenic | 35.0% |
| !! | TGAAGGACTTTTTCTTTGCT+GGG | - | chr8.1:70415807-70415826 | MS.gene019538:intron | 35.0% |
| !! | TGCACAATTTGATTTGTGCA+AGG | - | chr8.1:70415730-70415749 | MS.gene019538:intron | 35.0% |
| !! | TTGAAGGACTTTTTCTTTGC+TGG | - | chr8.1:70415806-70415825 | MS.gene019538:intron | 35.0% |
| !! | TTGGTGTTGCTGTCAAAATT+AGG | - | chr8.1:70416012-70416031 | MS.gene019538:CDS | 35.0% |
| AACCAGCTTTCGACAACAAT+AGG | + | chr8.1:70414937-70414956 | None:intergenic | 40.0% | |
| ACAACGCAACATTAACGAAG+TGG | - | chr8.1:70414715-70414734 | MS.gene019538:CDS | 40.0% | |
| ACATGCTATTCGTGTGCTAA+TGG | - | chr8.1:70416328-70416347 | MS.gene019538:CDS | 40.0% | |
| AGAAGATTTCTTCCTTTGCG+TGG | - | chr8.1:70415350-70415369 | MS.gene019538:CDS | 40.0% | |
| ATACGACTTTGATCAGCAGT+TGG | + | chr8.1:70414914-70414933 | None:intergenic | 40.0% | |
| ATCCTATTGTTGTCGAAAGC+TGG | - | chr8.1:70414932-70414951 | MS.gene019538:CDS | 40.0% | |
| ATGTCAGTAACATTGATGGG+TGG | - | chr8.1:70414504-70414523 | MS.gene019538:CDS | 40.0% | |
| CATCATACAAAAGTTGACGG+TGG | - | chr8.1:70416224-70416243 | MS.gene019538:CDS | 40.0% | |
| CCATCAATGTTACTGACATG+AGG | + | chr8.1:70414503-70414522 | None:intergenic | 40.0% | |
| CCTCATGTCAGTAACATTGA+TGG | - | chr8.1:70414500-70414519 | MS.gene019538:CDS | 40.0% | |
| CGAAAGAGAGTGGAAATCAA+TGG | - | chr8.1:70415706-70415725 | MS.gene019538:CDS | 40.0% | |
| CTCATGTTGGAGCAACTTAA+AGG | + | chr8.1:70416144-70416163 | None:intergenic | 40.0% | |
| CTTTCTTGTCTCCATCGAAA+AGG | + | chr8.1:70416389-70416408 | None:intergenic | 40.0% | |
| GAAGATTTCTTCCTTTGCGT+GGG | - | chr8.1:70415351-70415370 | MS.gene019538:CDS | 40.0% | |
| GAGCATATTATGTGGGCATT+TGG | - | chr8.1:70415175-70415194 | MS.gene019538:intron | 40.0% | |
| GAGTCTAAAAAGAACCCATC+TGG | - | chr8.1:70414647-70414666 | MS.gene019538:CDS | 40.0% | |
| GCAGCAACTGAAATTGACAA+TGG | - | chr8.1:70416185-70416204 | MS.gene019538:CDS | 40.0% | |
| GTTGACTACATGGCTAGATT+TGG | + | chr8.1:70416423-70416442 | None:intergenic | 40.0% | |
| TAGCATTGCAGTTGACTACA+TGG | + | chr8.1:70416433-70416452 | None:intergenic | 40.0% | |
| TGACTACATGGCTAGATTTG+GGG | + | chr8.1:70416421-70416440 | None:intergenic | 40.0% | |
| TTAATGGCTTCCTTAAGTGC+GGG | + | chr8.1:70415536-70415555 | None:intergenic | 40.0% | |
| TTGCTGGGATGACAACAATA+GGG | - | chr8.1:70415822-70415841 | MS.gene019538:CDS | 40.0% | |
| TTGTGGCAAAGTTGTCAAGT+TGG | - | chr8.1:70415600-70415619 | MS.gene019538:CDS | 40.0% | |
| TTTGCTGGGATGACAACAAT+AGG | - | chr8.1:70415821-70415840 | MS.gene019538:intron | 40.0% | |
| ! | AGGGAATTTGTTTGTCATCG+TGG | - | chr8.1:70414680-70414699 | MS.gene019538:CDS | 40.0% |
| ! | GAATTTGTTTGTCATCGTGG+TGG | - | chr8.1:70414683-70414702 | MS.gene019538:CDS | 40.0% |
| ! | TGATTAGTTCCACCACAACT+TGG | + | chr8.1:70416740-70416759 | None:intergenic | 40.0% |
| ! | TGGAGCAACTTAAAGGCATA+TGG | + | chr8.1:70416137-70416156 | None:intergenic | 40.0% |
| ! | TGGTAGTGATTGGCAATCTA+AGG | - | chr8.1:70414394-70414413 | MS.gene019538:CDS | 40.0% |
| ! | TGTCAGTAACATTGATGGGT+GGG | - | chr8.1:70414505-70414524 | MS.gene019538:CDS | 40.0% |
| !! | AACTTCCTAGCTTTTGTCCA+AGG | - | chr8.1:70415476-70415495 | MS.gene019538:CDS | 40.0% |
| !! | TGGGTTGGTGTTGATAATCA+CGG | - | chr8.1:70415289-70415308 | MS.gene019538:CDS | 40.0% |
| ACACCAACCCATATTCCAAG+TGG | + | chr8.1:70415280-70415299 | None:intergenic | 45.0% | |
| AGAGAACGAGTTGCTACTAG+AGG | - | chr8.1:70416518-70416537 | MS.gene019538:CDS | 45.0% | |
| AGAGGATCAAGACCAAAAGG+AGG | - | chr8.1:70416677-70416696 | MS.gene019538:CDS | 45.0% | |
| AGATCAAGATCCCGCACTTA+AGG | - | chr8.1:70415523-70415542 | MS.gene019538:CDS | 45.0% | |
| CCAAGAGGATCAAGACCAAA+AGG | - | chr8.1:70416674-70416693 | MS.gene019538:CDS | 45.0% | |
| CGGATGATTTCGAAAGAGAG+TGG | - | chr8.1:70415696-70415715 | MS.gene019538:CDS | 45.0% | |
| GATGCAGCATGTTCCTCAAT+AGG | + | chr8.1:70416104-70416123 | None:intergenic | 45.0% | |
| GTTGGAATGTTGCGATAAGC+AGG | + | chr8.1:70414896-70414915 | None:intergenic | 45.0% | |
| TAGGAATCAAGCTGGAGAAG+AGG | - | chr8.1:70416031-70416050 | MS.gene019538:CDS | 45.0% | |
| TGTCATGTTCCAAGTTGTGG+TGG | - | chr8.1:70416728-70416747 | MS.gene019538:CDS | 45.0% | |
| ! | ATGCCACTTGGAATATGGGT+TGG | - | chr8.1:70415274-70415293 | MS.gene019538:intron | 45.0% |
| ! | TTCCTTTGCGTGGGCTTTAA+AGG | - | chr8.1:70415360-70415379 | MS.gene019538:CDS | 45.0% |
| !! | ATGGGTGGGTTCCTTTTGAA+GGG | - | chr8.1:70414519-70414538 | MS.gene019538:CDS | 45.0% |
| !! | TACCTTTAAAGCCCACGCAA+AGG | + | chr8.1:70415365-70415384 | None:intergenic | 45.0% |
| !! | TGGCTTTGAGGAAAAATGGG+TGG | - | chr8.1:70414808-70414827 | MS.gene019538:CDS | 45.0% |
| !!! | CCTTTTGGTCTTGATCCTCT+TGG | + | chr8.1:70416677-70416696 | None:intergenic | 45.0% |
| AAATCCAAAGGTCGGCCAAG+AGG | - | chr8.1:70416659-70416678 | MS.gene019538:CDS | 50.0% | |
| CGCTGTCATGTTCCAAGTTG+TGG | - | chr8.1:70416725-70416744 | MS.gene019538:CDS | 50.0% | |
| GAGGAGTTGAAGCTGCAAAG+AGG | - | chr8.1:70416696-70416715 | MS.gene019538:CDS | 50.0% | |
| ! | GCAGCTTCAACTCCTCCTTT+TGG | + | chr8.1:70416692-70416711 | None:intergenic | 50.0% |
| !! | GATGGGTGGGTTCCTTTTGA+AGG | - | chr8.1:70414518-70414537 | MS.gene019538:CDS | 50.0% |
| ACGGTGGGCGTCAAGTAAGT+TGG | - | chr8.1:70416240-70416259 | MS.gene019538:CDS | 55.0% | |
| CTTGGAACATGACAGCGACG+AGG | + | chr8.1:70416722-70416741 | None:intergenic | 55.0% | |
| TGATCCTCTTGGCCGACCTT+TGG | + | chr8.1:70416666-70416685 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 70414374 | 70416829 | 70414374 | ID=MS.gene019538 |
| chr8.1 | mRNA | 70414374 | 70416829 | 70414374 | ID=MS.gene019538.t1;Parent=MS.gene019538 |
| chr8.1 | exon | 70415822 | 70416829 | 70415822 | ID=MS.gene019538.t1.exon1;Parent=MS.gene019538.t1 |
| chr8.1 | CDS | 70415822 | 70416829 | 70415822 | ID=cds.MS.gene019538.t1;Parent=MS.gene019538.t1 |
| chr8.1 | exon | 70415284 | 70415727 | 70415284 | ID=MS.gene019538.t1.exon2;Parent=MS.gene019538.t1 |
| chr8.1 | CDS | 70415284 | 70415727 | 70415284 | ID=cds.MS.gene019538.t1;Parent=MS.gene019538.t1 |
| chr8.1 | exon | 70414374 | 70415192 | 70414374 | ID=MS.gene019538.t1.exon3;Parent=MS.gene019538.t1 |
| chr8.1 | CDS | 70414374 | 70415192 | 70414374 | ID=cds.MS.gene019538.t1;Parent=MS.gene019538.t1 |
| Gene Sequence |
| Protein sequence |
