Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019580.t1 | XP_013444885.1 | 98.7 | 154 | 2 | 0 | 1 | 154 | 52 | 205 | 7.70E-81 | 309.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019580.t1 | Q1ECF5 | 64.4 | 149 | 53 | 0 | 1 | 149 | 52 | 200 | 1.9e-51 | 203.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019580.t1 | A0A072TZG0 | 98.7 | 154 | 2 | 0 | 1 | 154 | 52 | 205 | 5.5e-81 | 309.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene019580.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019580.t1 | MTR_8g031490 | 98.701 | 154 | 2 | 0 | 1 | 154 | 52 | 205 | 1.97e-112 | 317 |
| MS.gene019580.t1 | MTR_8g031490 | 53.659 | 82 | 37 | 1 | 46 | 126 | 3 | 84 | 1.33e-24 | 94.4 |
| MS.gene019580.t1 | MTR_7g076720 | 71.795 | 156 | 42 | 2 | 1 | 154 | 52 | 207 | 6.64e-74 | 220 |
| MS.gene019580.t1 | MTR_7g076720 | 57.746 | 71 | 30 | 0 | 46 | 116 | 3 | 73 | 3.76e-24 | 93.6 |
| MS.gene019580.t1 | MTR_3g070050 | 60.294 | 136 | 48 | 2 | 1 | 130 | 10 | 145 | 4.65e-57 | 175 |
| MS.gene019580.t1 | MTR_3g070050 | 60.294 | 136 | 48 | 2 | 1 | 130 | 52 | 187 | 3.14e-56 | 174 |
| MS.gene019580.t1 | MTR_3g070050 | 53.521 | 71 | 33 | 0 | 46 | 116 | 3 | 73 | 4.78e-22 | 87.4 |
| MS.gene019580.t1 | MTR_8g097300 | 53.947 | 152 | 64 | 3 | 1 | 151 | 52 | 198 | 2.92e-51 | 162 |
| MS.gene019580.t1 | MTR_8g097300 | 42.424 | 66 | 38 | 0 | 46 | 111 | 3 | 68 | 6.11e-15 | 69.3 |
| MS.gene019580.t1 | MTR_1g017950 | 45.139 | 144 | 72 | 3 | 1 | 138 | 52 | 194 | 1.74e-37 | 127 |
| MS.gene019580.t1 | MTR_1g017950 | 51.471 | 68 | 33 | 0 | 46 | 113 | 3 | 70 | 1.44e-18 | 78.6 |
| MS.gene019580.t1 | MTR_4g106800 | 44.961 | 129 | 58 | 2 | 1 | 120 | 53 | 177 | 1.36e-34 | 119 |
| MS.gene019580.t1 | MTR_4g106800 | 46.377 | 69 | 37 | 0 | 46 | 114 | 4 | 72 | 1.64e-16 | 72.8 |
| MS.gene019580.t1 | MTR_3g070033 | 56.923 | 65 | 28 | 0 | 66 | 130 | 6 | 70 | 1.27e-24 | 90.9 |
| MS.gene019580.t1 | MTR_1g106100 | 35.849 | 106 | 66 | 1 | 8 | 111 | 20 | 125 | 2.43e-18 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019580.t1 | AT3G61230 | 68.243 | 148 | 43 | 2 | 1 | 144 | 53 | 200 | 2.30e-72 | 216 |
| MS.gene019580.t1 | AT3G61230 | 45.833 | 96 | 48 | 1 | 46 | 141 | 4 | 95 | 2.38e-25 | 96.7 |
| MS.gene019580.t1 | AT2G45800 | 66.667 | 153 | 45 | 4 | 1 | 147 | 21 | 173 | 1.02e-68 | 206 |
| MS.gene019580.t1 | AT1G01780 | 64.430 | 149 | 53 | 0 | 1 | 149 | 52 | 200 | 3.53e-68 | 205 |
| MS.gene019580.t1 | AT1G01780 | 45.833 | 96 | 46 | 1 | 46 | 141 | 3 | 92 | 1.86e-24 | 94.0 |
| MS.gene019580.t1 | AT2G45800 | 66.667 | 153 | 45 | 4 | 1 | 147 | 52 | 204 | 4.25e-68 | 206 |
| MS.gene019580.t1 | AT2G45800 | 60.563 | 71 | 28 | 0 | 46 | 116 | 3 | 73 | 6.58e-26 | 98.6 |
| MS.gene019580.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene019580.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene019580.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene019580.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene019580.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene019580.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene019580.t1 | AT3G55770 | 67.460 | 126 | 37 | 2 | 1 | 122 | 52 | 177 | 1.59e-60 | 186 |
| MS.gene019580.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.30e-20 | 83.2 |
| MS.gene019580.t1 | AT2G39900 | 66.142 | 127 | 38 | 2 | 1 | 122 | 52 | 178 | 3.64e-60 | 185 |
| MS.gene019580.t1 | AT2G39900 | 48.101 | 79 | 41 | 0 | 46 | 124 | 3 | 81 | 8.36e-21 | 84.7 |
| MS.gene019580.t1 | AT3G55770 | 53.125 | 160 | 37 | 3 | 1 | 122 | 52 | 211 | 8.10e-53 | 167 |
| MS.gene019580.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 5.51e-20 | 83.2 |
| MS.gene019580.t1 | AT1G10200 | 45.161 | 124 | 62 | 2 | 1 | 118 | 10 | 133 | 1.35e-35 | 120 |
| MS.gene019580.t1 | AT1G10200 | 45.161 | 124 | 62 | 2 | 1 | 118 | 52 | 175 | 1.25e-34 | 119 |
| MS.gene019580.t1 | AT1G10200 | 50.000 | 68 | 34 | 0 | 46 | 113 | 3 | 70 | 1.88e-17 | 75.5 |
| MS.gene019580.t1 | AT3G55770 | 64.634 | 82 | 24 | 3 | 1 | 78 | 52 | 132 | 2.09e-28 | 102 |
| MS.gene019580.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 3.90e-21 | 84.0 |
| MS.gene019580.t1 | AT3G55770 | 65.789 | 76 | 20 | 3 | 1 | 70 | 52 | 127 | 8.81e-26 | 95.5 |
| MS.gene019580.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 46 | 143 | 3 | 98 | 2.19e-21 | 84.3 |
Find 28 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATTCAAGAGGTTTCTTC+TGG | 0.235129 | 8.1:+71245570 | None:intergenic |
| GTTTCTTCTGGATGATCTTC+AGG | 0.252303 | 8.1:+71245582 | None:intergenic |
| TCAAGCTATGCTGCTCTTGA+TGG | 0.353414 | 8.1:-71245750 | MS.gene019580:CDS |
| CAAAATGAACCCTGCAGTAT+AGG | 0.377575 | 8.1:+71245724 | None:intergenic |
| AGCAAGTCGCACATTTGTCC+AGG | 0.436853 | 8.1:+71246159 | None:intergenic |
| TGCTTTAGGTGTGCTCATGC+AGG | 0.437095 | 8.1:-71245789 | MS.gene019580:CDS |
| TTGATGGTGTCCTATACTGC+AGG | 0.448405 | 8.1:-71245734 | MS.gene019580:CDS |
| TCTACCACAAGACATGCTTT+AGG | 0.453453 | 8.1:-71245803 | MS.gene019580:CDS |
| AACCGTCTATCCACTGGAAA+AGG | 0.456725 | 8.1:-71246132 | MS.gene019580:intron |
| CTCTAAAACCGTCTATCCAC+TGG | 0.500295 | 8.1:-71246138 | MS.gene019580:CDS |
| TTGTTACTGTTTCATTCAAG+AGG | 0.524503 | 8.1:+71245560 | None:intergenic |
| TTCCATGTTCAGTGGTACCC+TGG | 0.525905 | 8.1:-71246177 | MS.gene019580:CDS |
| AACATGGAAGATAGTCTACT+TGG | 0.545011 | 8.1:+71246191 | None:intergenic |
| GGTGCTTCTGCTGATTGTGA+AGG | 0.545791 | 8.1:+71245603 | None:intergenic |
| ATTAAACAGGTGACACTGGA+AGG | 0.549370 | 8.1:-71245831 | MS.gene019580:intron |
| GCAATAGAGAATTCCATCCA+TGG | 0.558350 | 8.1:+71246755 | None:intergenic |
| AGTACATACTCCTCCATGGA+TGG | 0.562913 | 8.1:-71246768 | None:intergenic |
| TTCTGACAAAGCAAATGAGC+TGG | 0.565336 | 8.1:-71246539 | MS.gene019580:intron |
| TACTCAAATCTGCACACAAG+AGG | 0.573033 | 8.1:-71245665 | MS.gene019580:CDS |
| GCTTTAGGTGTGCTCATGCA+GGG | 0.590942 | 8.1:-71245788 | MS.gene019580:CDS |
| TGATGGTGTCCTATACTGCA+GGG | 0.598936 | 8.1:-71245733 | MS.gene019580:CDS |
| GTCCAGGGTACCACTGAACA+TGG | 0.612761 | 8.1:+71246175 | None:intergenic |
| GCATAGCTTGAATGTGTGAG+AGG | 0.618734 | 8.1:+71245762 | None:intergenic |
| AAATCTGCACACAAGAGGAA+TGG | 0.618865 | 8.1:-71245660 | MS.gene019580:CDS |
| CATAGCTTGAATGTGTGAGA+GGG | 0.638300 | 8.1:+71245763 | None:intergenic |
| AGACTATCTTCCATGTTCAG+TGG | 0.654978 | 8.1:-71246185 | MS.gene019580:CDS |
| GCAAGTCGCACATTTGTCCA+GGG | 0.661497 | 8.1:+71246160 | None:intergenic |
| CACACCTAAAGCATGTCTTG+TGG | 0.697099 | 8.1:+71245799 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATCTCTAATAATTTA+TGG | + | chr8.1:71245958-71245977 | None:intergenic | 10.0% |
| !! | TAAAATAAAAATTCAAGTAA+TGG | + | chr8.1:71245858-71245877 | None:intergenic | 10.0% |
| !!! | AATTGTTTCTTTTAAAAATT+TGG | - | chr8.1:71246283-71246302 | MS.gene019580:intron | 10.0% |
| !!! | ATATAATTTTTTGTTCATTT+TGG | - | chr8.1:71245733-71245752 | MS.gene019580:CDS | 10.0% |
| !! | AAAATAAAAAATGAAGGATT+TGG | + | chr8.1:71245660-71245679 | None:intergenic | 15.0% |
| !! | AAAATTATATGTGTATCAAT+TGG | + | chr8.1:71245723-71245742 | None:intergenic | 15.0% |
| !! | TAAAAGATAAAGATAAGATA+TGG | + | chr8.1:71245821-71245840 | None:intergenic | 15.0% |
| !!! | TTTTTTTGGTTTTATTAAAC+AGG | - | chr8.1:71246484-71246503 | MS.gene019580:intron | 15.0% |
| !! | AATATGGAATTCATAAATGA+AGG | - | chr8.1:71246422-71246441 | MS.gene019580:intron | 20.0% |
| !!! | AGACAAATTTCATATCTTTT+TGG | + | chr8.1:71245926-71245945 | None:intergenic | 20.0% |
| !!! | GCTTTTTTAAATCAAATTCT+TGG | + | chr8.1:71246336-71246355 | None:intergenic | 20.0% |
| ! | AAAAAGTTGAATTAGCCATA+TGG | - | chr8.1:71245890-71245909 | MS.gene019580:intron | 25.0% |
| ! | AATTAGCCATATGGAAATTA+GGG | - | chr8.1:71245899-71245918 | MS.gene019580:intron | 25.0% |
| ! | TTACCTGATTTCATATAACT+TGG | - | chr8.1:71246309-71246328 | MS.gene019580:intron | 25.0% |
| ! | TTTAAACACAAGCTGTAATA+TGG | - | chr8.1:71246406-71246425 | MS.gene019580:intron | 25.0% |
| !! | AAAAAATGTGTTACGACTTT+TGG | + | chr8.1:71246470-71246489 | None:intergenic | 25.0% |
| !! | AACAATTTTCCACAAAATCA+AGG | + | chr8.1:71246270-71246289 | None:intergenic | 25.0% |
| !!! | AAAGTCGTAACACATTTTTT+TGG | - | chr8.1:71246470-71246489 | MS.gene019580:intron | 25.0% |
| !!! | GTATTGATCTTTTACAGAAT+AGG | - | chr8.1:71246111-71246130 | MS.gene019580:intron | 25.0% |
| GAATTAGCCATATGGAAATT+AGG | - | chr8.1:71245898-71245917 | MS.gene019580:intron | 30.0% | |
| ! | ACATTTTGAACAGCTTTTCA+AGG | - | chr8.1:71245601-71245620 | MS.gene019580:CDS | 30.0% |
| ! | TCATTTTGCACAACTTTTCA+TGG | - | chr8.1:71246619-71246638 | MS.gene019580:intron | 30.0% |
| ! | TTCATAAATGAAGGTGCATT+CGG | - | chr8.1:71246431-71246450 | MS.gene019580:intron | 30.0% |
| ! | TTTTATTAAACAGGTGACAC+TGG | - | chr8.1:71246493-71246512 | MS.gene019580:intron | 30.0% |
| AAAGCAATCATACGTTTCGT+TGG | - | chr8.1:71246351-71246370 | MS.gene019580:intron | 35.0% | |
| AACATGGAAGATAGTCTACT+TGG | + | chr8.1:71246140-71246159 | None:intergenic | 35.0% | |
| ATCATACGTTTCGTTGGATA+TGG | - | chr8.1:71246357-71246376 | MS.gene019580:intron | 35.0% | |
| GGTCCAAGTTATATGAAATC+AGG | + | chr8.1:71246315-71246334 | None:intergenic | 35.0% | |
| TTTGGTCCCTAATTTCCATA+TGG | + | chr8.1:71245908-71245927 | None:intergenic | 35.0% | |
| ! | TGTTGTGATCCTTGATTTTG+TGG | - | chr8.1:71246258-71246277 | MS.gene019580:intron | 35.0% |
| !! | GGCAATTTTAGCAAGAACTT+TGG | - | chr8.1:71245629-71245648 | MS.gene019580:CDS | 35.0% |
| AAATCTGCACACAAGAGGAA+TGG | - | chr8.1:71246668-71246687 | MS.gene019580:intron | 40.0% | |
| AGACTATCTTCCATGTTCAG+TGG | - | chr8.1:71246143-71246162 | MS.gene019580:CDS | 40.0% | |
| ATTAAACAGGTGACACTGGA+AGG | - | chr8.1:71246497-71246516 | MS.gene019580:intron | 40.0% | |
| CAAAATGAACCCTGCAGTAT+AGG | + | chr8.1:71246607-71246626 | None:intergenic | 40.0% | |
| CATAGCTTGAATGTGTGAGA+GGG | + | chr8.1:71246568-71246587 | None:intergenic | 40.0% | |
| GTTTCTTCTGGATGATCTTC+AGG | + | chr8.1:71246749-71246768 | None:intergenic | 40.0% | |
| TACTCAAATCTGCACACAAG+AGG | - | chr8.1:71246663-71246682 | MS.gene019580:intron | 40.0% | |
| TCTACCACAAGACATGCTTT+AGG | - | chr8.1:71246525-71246544 | MS.gene019580:intron | 40.0% | |
| TTCTGACAAAGCAAATGAGC+TGG | - | chr8.1:71245789-71245808 | MS.gene019580:CDS | 40.0% | |
| ! | AACCTTTTCCAGTGGATAGA+CGG | + | chr8.1:71246201-71246220 | None:intergenic | 40.0% |
| ! | GCACAACTTTTCATGGAGAA+AGG | - | chr8.1:71246626-71246645 | MS.gene019580:intron | 40.0% |
| ! | TGTCGCGATTCTTGATTTTG+AGG | - | chr8.1:71246221-71246240 | MS.gene019580:intron | 40.0% |
| ! | TGTGTTACGACTTTTGGTCA+CGG | + | chr8.1:71246464-71246483 | None:intergenic | 40.0% |
| !! | GAACAGCTTTTCAAGGAATC+TGG | - | chr8.1:71245608-71245627 | MS.gene019580:CDS | 40.0% |
| AACCGTCTATCCACTGGAAA+AGG | - | chr8.1:71246196-71246215 | MS.gene019580:CDS | 45.0% | |
| CACACCTAAAGCATGTCTTG+TGG | + | chr8.1:71246532-71246551 | None:intergenic | 45.0% | |
| GCATAGCTTGAATGTGTGAG+AGG | + | chr8.1:71246569-71246588 | None:intergenic | 45.0% | |
| TCAAGCTATGCTGCTCTTGA+TGG | - | chr8.1:71246578-71246597 | MS.gene019580:intron | 45.0% | |
| ! | TGATGGTGTCCTATACTGCA+GGG | - | chr8.1:71246595-71246614 | MS.gene019580:intron | 45.0% |
| !! | CTCTAAAACCGTCTATCCAC+TGG | - | chr8.1:71246190-71246209 | MS.gene019580:CDS | 45.0% |
| !! | TTGATGGTGTCCTATACTGC+AGG | - | chr8.1:71246594-71246613 | MS.gene019580:intron | 45.0% |
| !! | AAAATTAAAATAAAAAATGA+AGG | + | chr8.1:71245666-71245685 | None:intergenic | 5.0% |
| AGCAAGTCGCACATTTGTCC+AGG | + | chr8.1:71246172-71246191 | None:intergenic | 50.0% | |
| GCAAGTCGCACATTTGTCCA+GGG | + | chr8.1:71246171-71246190 | None:intergenic | 50.0% | |
| TTCCATGTTCAGTGGTACCC+TGG | - | chr8.1:71246151-71246170 | MS.gene019580:CDS | 50.0% | |
| ! | CGACTTTTGGTCACGGAGTT+TGG | + | chr8.1:71246457-71246476 | None:intergenic | 50.0% |
| ! | GCTTTAGGTGTGCTCATGCA+GGG | - | chr8.1:71246540-71246559 | MS.gene019580:CDS | 50.0% |
| ! | GGTGCTTCTGCTGATTGTGA+AGG | + | chr8.1:71246728-71246747 | None:intergenic | 50.0% |
| ! | TCGCGACAAACCTTTTCCAG+TGG | + | chr8.1:71246209-71246228 | None:intergenic | 50.0% |
| ! | TGCTTTAGGTGTGCTCATGC+AGG | - | chr8.1:71246539-71246558 | MS.gene019580:intron | 50.0% |
| ! | GTCCAGGGTACCACTGAACA+TGG | + | chr8.1:71246156-71246175 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 71245575 | 71246775 | 71245575 | ID=MS.gene019580 |
| chr8.1 | mRNA | 71245575 | 71246775 | 71245575 | ID=MS.gene019580.t1;Parent=MS.gene019580 |
| chr8.1 | exon | 71246700 | 71246775 | 71246700 | ID=MS.gene019580.t1.exon1;Parent=MS.gene019580.t1 |
| chr8.1 | CDS | 71246700 | 71246775 | 71246700 | ID=cds.MS.gene019580.t1;Parent=MS.gene019580.t1 |
| chr8.1 | exon | 71246540 | 71246568 | 71246540 | ID=MS.gene019580.t1.exon2;Parent=MS.gene019580.t1 |
| chr8.1 | CDS | 71246540 | 71246568 | 71246540 | ID=cds.MS.gene019580.t1;Parent=MS.gene019580.t1 |
| chr8.1 | exon | 71246133 | 71246222 | 71246133 | ID=MS.gene019580.t1.exon3;Parent=MS.gene019580.t1 |
| chr8.1 | CDS | 71246133 | 71246222 | 71246133 | ID=cds.MS.gene019580.t1;Parent=MS.gene019580.t1 |
| chr8.1 | exon | 71245575 | 71245844 | 71245575 | ID=MS.gene019580.t1.exon4;Parent=MS.gene019580.t1 |
| chr8.1 | CDS | 71245575 | 71245844 | 71245575 | ID=cds.MS.gene019580.t1;Parent=MS.gene019580.t1 |
| Gene Sequence |
| Protein sequence |
