Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020070.t1 | GAU37750.1 | 43.4 | 122 | 68 | 1 | 1 | 121 | 88 | 209 | 2.90E-19 | 104.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020070.t1 | A0A2Z6NMK1 | 43.4 | 122 | 68 | 1 | 1 | 121 | 88 | 209 | 2.1e-19 | 104.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020070.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATGTGATGTACTAATA+AGG | 0.256110 | 8.2:+79587068 | MS.gene020070:CDS |
| CAAATGTAATGAAGGTTTCT+AGG | 0.290265 | 8.2:+79587203 | MS.gene020070:CDS |
| TGATAACCTGCAGCCATATA+AGG | 0.324757 | 8.2:-79586881 | None:intergenic |
| AATGATCTTAACTCCTTATA+TGG | 0.327626 | 8.2:+79586868 | MS.gene020070:CDS |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 8.2:+79587168 | MS.gene020070:CDS |
| GAGTTAAGATCATTTCATAA+TGG | 0.420070 | 8.2:-79586859 | None:intergenic |
| GGTGATTACATTAGAAGCTC+AGG | 0.426576 | 8.2:+79587004 | MS.gene020070:CDS |
| CAGACACTGAACATTGACTT+TGG | 0.433491 | 8.2:+79586983 | MS.gene020070:intron |
| CAAATGTGATGTACTAATAA+GGG | 0.442101 | 8.2:+79587069 | MS.gene020070:CDS |
| TATGGCTGCAGGTTATCAAT+TGG | 0.445570 | 8.2:+79586886 | MS.gene020070:CDS |
| TACTCTGCTATTAATATGTA+TGG | 0.453609 | 8.2:+79587028 | MS.gene020070:CDS |
| TTAACTCCTTATATGGCTGC+AGG | 0.483063 | 8.2:+79586875 | MS.gene020070:CDS |
| ATTAATATGTATGGACCTAC+AGG | 0.484413 | 8.2:+79587037 | MS.gene020070:CDS |
| TGTAAAGACAATGGATTCCA+TGG | 0.503947 | 8.2:+79587139 | MS.gene020070:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.515012 | 8.2:+79586829 | MS.gene020070:CDS |
| AAGTACATCAAATGTAATGA+AGG | 0.524181 | 8.2:+79587195 | MS.gene020070:CDS |
| ATGTCAACTCATTGCCATCA+TGG | 0.539340 | 8.2:+79586804 | MS.gene020070:CDS |
| AAGACAATGGATTCCATGGT+GGG | 0.554852 | 8.2:+79587143 | MS.gene020070:CDS |
| TGCTTGTAAGTGTGCCATGA+TGG | 0.567390 | 8.2:-79586818 | None:intergenic |
| AAGGCAACAAAGATTGGCAG+TGG | 0.574230 | 8.2:+79587103 | MS.gene020070:CDS |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 8.2:-79587052 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 8.2:-79587062 | None:intergenic |
| TGTGATGTACTAATAAGGGA+TGG | 0.597036 | 8.2:+79587073 | MS.gene020070:CDS |
| CAACAAAGATTGGCAGTGGG+TGG | 0.601376 | 8.2:+79587107 | MS.gene020070:CDS |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 8.2:-79587156 | None:intergenic |
| AAAGACAATGGATTCCATGG+TGG | 0.635851 | 8.2:+79587142 | MS.gene020070:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.640570 | 8.2:+79587144 | MS.gene020070:CDS |
| TCATGGCACACTTACAAGCA+AGG | 0.641070 | 8.2:+79586821 | MS.gene020070:CDS |
| AGGCAACAAAGATTGGCAGT+GGG | 0.670518 | 8.2:+79587104 | MS.gene020070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAAATTTTGTAAAGACAA+TGG | + | chr8.2:79587130-79587149 | MS.gene020070:CDS | 20.0% |
| ! | AAGTACATCAAATGTAATGA+AGG | + | chr8.2:79587195-79587214 | MS.gene020070:CDS | 25.0% |
| ! | AATGATCTTAACTCCTTATA+TGG | + | chr8.2:79586868-79586887 | MS.gene020070:CDS | 25.0% |
| ! | ACAAATGTGATGTACTAATA+AGG | + | chr8.2:79587068-79587087 | MS.gene020070:CDS | 25.0% |
| ! | CAAATGTGATGTACTAATAA+GGG | + | chr8.2:79587069-79587088 | MS.gene020070:CDS | 25.0% |
| ! | GAGTTAAGATCATTTCATAA+TGG | - | chr8.2:79586862-79586881 | None:intergenic | 25.0% |
| ! | TACTCTGCTATTAATATGTA+TGG | + | chr8.2:79587028-79587047 | MS.gene020070:CDS | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | + | chr8.2:79587168-79587187 | MS.gene020070:CDS | 25.0% |
| !! | TAGAGATGTATCATTTTAGA+AGG | - | chr8.2:79586927-79586946 | None:intergenic | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | + | chr8.2:79587097-79587116 | MS.gene020070:CDS | 30.0% | |
| AATAAGGGATGGAAAAAAGA+AGG | + | chr8.2:79587084-79587103 | MS.gene020070:CDS | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | + | chr8.2:79587037-79587056 | MS.gene020070:CDS | 30.0% | |
| CAAATGTAATGAAGGTTTCT+AGG | + | chr8.2:79587203-79587222 | MS.gene020070:CDS | 30.0% | |
| TAGTACATCACATTTGTACT+CGG | - | chr8.2:79587065-79587084 | None:intergenic | 30.0% | |
| !! | ATCATTTTAGAAGGAGTTGT+TGG | - | chr8.2:79586918-79586937 | None:intergenic | 30.0% |
| TGTGATGTACTAATAAGGGA+TGG | + | chr8.2:79587073-79587092 | MS.gene020070:CDS | 35.0% | |
| ! | TGTAAAGACAATGGATTCCA+TGG | + | chr8.2:79587139-79587158 | MS.gene020070:CDS | 35.0% |
| ATGTCAACTCATTGCCATCA+TGG | + | chr8.2:79586804-79586823 | MS.gene020070:CDS | 40.0% | |
| CACTTACAAGCAAGGAATTG+AGG | + | chr8.2:79586829-79586848 | MS.gene020070:CDS | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | + | chr8.2:79587004-79587023 | MS.gene020070:CDS | 40.0% | |
| TATGGCTGCAGGTTATCAAT+TGG | + | chr8.2:79586886-79586905 | MS.gene020070:CDS | 40.0% | |
| TGATAACCTGCAGCCATATA+AGG | - | chr8.2:79586884-79586903 | None:intergenic | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | + | chr8.2:79586875-79586894 | MS.gene020070:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | + | chr8.2:79587142-79587161 | MS.gene020070:CDS | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | + | chr8.2:79587143-79587162 | MS.gene020070:CDS | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | + | chr8.2:79586983-79587002 | MS.gene020070:intron | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | + | chr8.2:79587103-79587122 | MS.gene020070:CDS | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | - | chr8.2:79587159-79587178 | None:intergenic | 45.0% | |
| AGGCAACAAAGATTGGCAGT+GGG | + | chr8.2:79587104-79587123 | MS.gene020070:CDS | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | + | chr8.2:79586821-79586840 | MS.gene020070:CDS | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | - | chr8.2:79586821-79586840 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | + | chr8.2:79587144-79587163 | MS.gene020070:CDS | 45.0% |
| CAACAAAGATTGGCAGTGGG+TGG | + | chr8.2:79587107-79587126 | MS.gene020070:CDS | 50.0% | |
| !! | CATTTGTACTCGGTGCCTGT+AGG | - | chr8.2:79587055-79587074 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 79586788 | 79587231 | 79586788 | ID=MS.gene020070 |
| chr8.2 | mRNA | 79586788 | 79587231 | 79586788 | ID=MS.gene020070.t1;Parent=MS.gene020070 |
| chr8.2 | exon | 79586788 | 79586907 | 79586788 | ID=MS.gene020070.t1.exon1;Parent=MS.gene020070.t1 |
| chr8.2 | CDS | 79586788 | 79586907 | 79586788 | ID=cds.MS.gene020070.t1;Parent=MS.gene020070.t1 |
| chr8.2 | exon | 79586986 | 79587231 | 79586986 | ID=MS.gene020070.t1.exon2;Parent=MS.gene020070.t1 |
| chr8.2 | CDS | 79586986 | 79587231 | 79586986 | ID=cds.MS.gene020070.t1;Parent=MS.gene020070.t1 |
| Gene Sequence |
| Protein sequence |
