Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020107.t1 | XP_003626931.1 | 91.3 | 104 | 9 | 0 | 2 | 105 | 30 | 133 | 1.00E-47 | 199.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020107.t1 | G7LF49 | 91.3 | 104 | 9 | 0 | 2 | 105 | 30 | 133 | 7.2e-48 | 199.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020107.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020107.t1 | MTR_8g012210 | 91.346 | 104 | 9 | 0 | 2 | 105 | 30 | 133 | 3.84e-66 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020107.t1 | AT1G79220 | 48.684 | 76 | 39 | 0 | 16 | 91 | 72 | 147 | 5.68e-20 | 83.2 |
Find 0 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTAAAGATTATCAATTGT+AGG | + | 57568:5592-5611 | MS.gene020107:CDS | 20.0% |
| !!! | TACTGAAGTTTTGAGTAAAT+GGG | + | 57568:5446-5465 | MS.gene020107:CDS | 25.0% |
| AAATGTTGAAGACAAATGGA+AGG | + | 57568:5419-5438 | MS.gene020107:CDS | 30.0% | |
| TGCAAAATGTTGAAGACAAA+TGG | + | 57568:5415-5434 | MS.gene020107:CDS | 30.0% | |
| !!! | ACTGAAGTTTTGAGTAAATG+GGG | + | 57568:5447-5466 | MS.gene020107:CDS | 30.0% |
| !!! | CTACTGAAGTTTTGAGTAAA+TGG | + | 57568:5445-5464 | MS.gene020107:CDS | 30.0% |
| ! | ATTTCAACCTCATTTTGAGC+TGG | - | 57568:5396-5415 | MS.gene020107:intergenic | 35.0% |
| ! | TTGCTTAGTGATTTGGGTTT+AGG | + | 57568:5558-5577 | MS.gene020107:CDS | 35.0% |
| ! | TTTCAACCTCATTTTGAGCT+GGG | - | 57568:5395-5414 | MS.gene020107:intergenic | 35.0% |
| ACTTCGCTTGCTTAGTGATT+TGG | + | 57568:5551-5570 | MS.gene020107:CDS | 40.0% | |
| CTTCGCTTGCTTAGTGATTT+GGG | + | 57568:5552-5571 | MS.gene020107:CDS | 40.0% | |
| TCAAGCTCTCGTCGAAATTA+TGG | - | 57568:5644-5663 | MS.gene020107:intergenic | 40.0% | |
| ! | ATTGTAGGCCTCGATTTTTC+AGG | + | 57568:5607-5626 | MS.gene020107:CDS | 40.0% |
| !! | GTTTTGAGTAAATGGGGTTG+TGG | + | 57568:5453-5472 | MS.gene020107:CDS | 40.0% |
| AGTCGAGTCCTGAAAAATCG+AGG | - | 57568:5618-5637 | MS.gene020107:intergenic | 45.0% | |
| AGTTTCGACTGAACCTGAGA+AGG | - | 57568:5534-5553 | MS.gene020107:intergenic | 45.0% | |
| GAAAGTCCCAGCTCAAAATG+AGG | + | 57568:5386-5405 | MS.gene020107:CDS | 45.0% | |
| ACTTTCGTCGAATAGGTGGC+AGG | - | 57568:5372-5391 | MS.gene020107:intergenic | 50.0% | |
| AGCTGGGACTTTCGTCGAAT+AGG | - | 57568:5379-5398 | MS.gene020107:intergenic | 50.0% | |
| TGGGACTTTCGTCGAATAGG+TGG | - | 57568:5376-5395 | MS.gene020107:intergenic | 50.0% | |
| ! | GGGTTGTGGTGATGACGATT+TGG | + | 57568:5467-5486 | MS.gene020107:CDS | 50.0% |
| GATCCGCATTGCGCAAAGAC+GGG | - | 57568:5512-5531 | MS.gene020107:intergenic | 55.0% | |
| GGATCCGCATTGCGCAAAGA+CGG | - | 57568:5513-5532 | MS.gene020107:intergenic | 55.0% | |
| TTGCCCGTCTTTGCGCAATG+CGG | + | 57568:5506-5525 | MS.gene020107:CDS | 55.0% | |
| GCGCAATGCGGATCCTTCTC+AGG | + | 57568:5518-5537 | MS.gene020107:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57568 | gene | 5366 | 5683 | 5366 | ID=MS.gene020107 |
| 57568 | mRNA | 5366 | 5683 | 5366 | ID=MS.gene020107.t1;Parent=MS.gene020107 |
| 57568 | exon | 5366 | 5683 | 5366 | ID=MS.gene020107.t1.exon1;Parent=MS.gene020107.t1 |
| 57568 | CDS | 5366 | 5683 | 5366 | ID=cds.MS.gene020107.t1;Parent=MS.gene020107.t1 |
| Gene Sequence |
| Protein sequence |
