AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02020.t1 MTR_6g051425 30.075 266 177 3 88 344 20 285 1.62e-37 143
MS.gene02020.t1 MTR_1g070355 29.682 283 179 10 44 314 34 308 2.84e-21 99.0
MS.gene02020.t1 MTR_4g067290 36.538 104 63 1 84 184 55 158 3.18e-15 76.3
MS.gene02020.t1 MTR_4g067290 36.538 104 63 1 84 184 55 158 3.20e-15 76.3
MS.gene02020.t1 MTR_4g067290 36.538 104 63 1 84 184 75 178 4.46e-15 76.3
MS.gene02020.t1 MTR_4g067290 36.538 104 63 1 84 184 73 176 4.72e-15 76.3
MS.gene02020.t1 MTR_4g067290 36.538 104 63 1 84 184 75 178 4.78e-15 76.3
MS.gene02020.t1 MTR_5g076070 26.408 284 174 10 43 309 17 282 5.18e-15 76.6
MS.gene02020.t1 MTR_4g067290 36.538 104 63 1 84 184 115 218 6.08e-15 76.6
MS.gene02020.t1 MTR_2g098920 34.014 147 90 3 78 219 75 219 1.02e-14 75.5
MS.gene02020.t1 MTR_0558s0020 25.843 267 148 10 91 320 42 295 3.68e-14 74.3
MS.gene02020.t1 MTR_7g446520 25.714 245 163 5 83 323 86 315 6.67e-14 73.9
MS.gene02020.t1 MTR_8g446020 28.649 185 110 6 148 320 81 255 8.22e-13 69.7
MS.gene02020.t1 MTR_2g098880 29.032 155 94 3 34 182 20 164 1.42e-12 68.9
MS.gene02020.t1 MTR_2g098880 29.032 155 94 3 34 182 32 176 1.70e-12 68.9
MS.gene02020.t1 MTR_7g446510 29.213 178 114 3 160 329 156 329 1.42e-11 67.0
MS.gene02020.t1 MTR_7g446480 28.736 174 120 2 160 329 141 314 4.79e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02020.t1 AT1G80010 53.528 652 286 7 64 703 74 720 0.0 696
MS.gene02020.t1 AT1G52520 50.147 678 297 13 48 704 42 699 0.0 643
MS.gene02020.t1 AT4G15090 33.387 620 398 6 84 697 50 660 2.81e-120 380
MS.gene02020.t1 AT4G15090 33.387 620 398 6 84 697 50 660 2.81e-120 380
MS.gene02020.t1 AT4G15090 33.387 620 398 6 84 697 50 660 2.81e-120 380
MS.gene02020.t1 AT4G15090 33.387 620 398 6 84 697 50 660 2.81e-120 380
MS.gene02020.t1 AT4G15090 33.387 620 398 6 84 697 50 660 2.81e-120 380
MS.gene02020.t1 AT1G76320 34.138 621 383 9 89 703 1 601 3.76e-112 354
MS.gene02020.t1 AT1G76320 34.138 621 383 9 89 703 1 601 1.33e-111 355
MS.gene02020.t1 AT1G76320 34.138 621 383 9 89 703 1 601 1.33e-111 355
MS.gene02020.t1 AT1G76320 34.138 621 383 9 89 703 1 601 1.46e-111 354
MS.gene02020.t1 AT3G22170 29.250 653 412 7 84 718 69 689 2.35e-104 338
MS.gene02020.t1 AT3G22170 29.250 653 412 7 84 718 69 689 2.35e-104 338
MS.gene02020.t1 AT4G38180 32.982 570 366 8 84 644 72 634 1.91e-103 335
MS.gene02020.t1 AT2G27110 29.586 676 417 15 36 704 16 639 2.21e-92 306
MS.gene02020.t1 AT2G27110 29.586 676 417 15 36 704 16 639 2.21e-92 306
MS.gene02020.t1 AT5G18960 29.430 632 402 9 70 700 196 784 8.79e-90 298
MS.gene02020.t1 AT5G18960 30.159 126 79 1 84 209 42 158 9.65e-13 72.4
MS.gene02020.t1 AT5G18960 29.430 632 402 9 70 700 196 784 8.79e-90 298
MS.gene02020.t1 AT5G18960 30.159 126 79 1 84 209 42 158 9.65e-13 72.4
MS.gene02020.t1 AT3G06250 28.834 652 408 10 58 700 156 760 7.62e-88 292
MS.gene02020.t1 AT3G06250 36.082 97 53 1 84 180 27 114 7.84e-13 72.4
MS.gene02020.t1 AT3G06250 28.834 652 408 10 58 700 156 760 7.62e-88 292
MS.gene02020.t1 AT3G06250 36.082 97 53 1 84 180 27 114 7.84e-13 72.4
MS.gene02020.t1 AT3G06250 28.834 652 408 10 58 700 156 760 7.62e-88 292
MS.gene02020.t1 AT3G06250 36.082 97 53 1 84 180 27 114 7.84e-13 72.4
MS.gene02020.t1 AT2G32250 31.655 556 332 6 88 641 41 550 1.04e-87 292
MS.gene02020.t1 AT2G32250 31.655 556 332 6 88 641 41 550 2.06e-87 292
MS.gene02020.t1 AT2G32250 31.655 556 332 6 88 641 41 550 2.06e-87 292
MS.gene02020.t1 AT2G32250 31.362 558 331 7 88 641 41 550 2.60e-87 292
MS.gene02020.t1 AT2G32250 31.362 558 331 7 88 641 41 550 2.60e-87 292
MS.gene02020.t1 AT2G32250 31.362 558 331 7 88 641 41 550 2.60e-87 292
MS.gene02020.t1 AT2G32250 31.239 557 331 7 89 641 1 509 7.16e-87 290
MS.gene02020.t1 AT2G32250 31.532 555 332 6 89 641 1 509 9.47e-87 290
MS.gene02020.t1 AT2G27110 32.645 484 305 7 228 704 25 494 1.24e-84 282
MS.gene02020.t1 AT4G19990 30.236 592 350 10 87 658 23 571 1.24e-83 281
MS.gene02020.t1 AT4G19990 30.236 592 350 10 87 658 23 571 1.24e-83 281
MS.gene02020.t1 AT4G19990 30.236 592 350 10 87 658 23 571 1.82e-83 280
MS.gene02020.t1 AT4G19990 29.853 613 367 10 66 658 16 585 1.98e-83 281
MS.gene02020.t1 AT4G19990 30.236 592 350 10 87 658 23 571 2.69e-83 280
MS.gene02020.t1 AT4G19990 30.236 592 350 10 87 658 23 571 2.69e-83 280
MS.gene02020.t1 AT4G19990 30.813 529 317 9 136 658 29 514 5.21e-76 259
MS.gene02020.t1 AT4G38170 30.998 471 296 9 256 715 19 471 8.93e-72 244
MS.gene02020.t1 AT1G10240 25.389 579 392 13 83 641 46 604 4.35e-53 195
MS.gene02020.t1 AT1G10240 25.389 579 392 13 83 641 46 604 4.35e-53 195
MS.gene02020.t1 AT5G28530 26.500 600 385 18 85 655 55 627 3.98e-46 175
MS.gene02020.t1 AT5G28530 26.167 600 383 18 85 655 55 623 2.67e-45 173
MS.gene02020.t1 AT5G28530 26.167 600 383 18 85 655 55 623 2.67e-45 173
MS.gene02020.t1 AT5G28530 26.488 336 217 9 327 655 252 564 2.54e-24 108
MS.gene02020.t1 AT5G28530 25.893 336 215 9 327 655 252 560 1.73e-23 106
MS.gene02020.t1 AT3G59470 37.037 108 65 1 84 188 69 176 3.89e-15 76.3
MS.gene02020.t1 AT3G59470 37.037 108 65 1 84 188 69 176 3.89e-15 76.3
MS.gene02020.t1 AT3G59470 37.037 108 65 1 84 188 81 188 4.50e-15 76.3

Find 151 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAACCAAATAATGATTT+TGG 0.088603 2.2:+59027088 None:intergenic
GACAATTGAGGAACATATTT+TGG 0.117184 2.2:-59028289 MS.gene02020:CDS
GATATCCCAAAATCATTATT+TGG 0.175601 2.2:-59027094 MS.gene02020:CDS
TGGTTGTCTGGTGCAGTTTC+AGG 0.204396 2.2:-59027934 MS.gene02020:CDS
AATAGCTATGCCAGGGAAAT+TGG 0.208039 2.2:-59028830 MS.gene02020:CDS
AGGGCTGCATATAGTTATTT+TGG 0.210404 2.2:-59028251 MS.gene02020:CDS
AAAATCATTATTTGGTTTCT+TGG 0.223441 2.2:-59027086 MS.gene02020:CDS
TTTGGATTGATTCTAGTTCT+AGG 0.231462 2.2:-59028271 MS.gene02020:CDS
AACTGAAGCTCTTGATGATT+TGG 0.234723 2.2:-59027604 MS.gene02020:CDS
CGGTTTGTAACAATAGTTTC+TGG 0.236513 2.2:+59028044 None:intergenic
AATTAGGACTCACCAGTTTA+TGG 0.247549 2.2:+59028342 None:intergenic
GGGAAATTGGATTTGCTATT+CGG 0.274552 2.2:-59028817 MS.gene02020:CDS
ATTTATAGAGTCTTTGAATA+AGG 0.281326 2.2:-59027058 MS.gene02020:intron
TAGATGAATTTGAAAGAGAT+TGG 0.315956 2.2:-59027857 MS.gene02020:CDS
AGTCGGCTCAACCTTTCTTT+TGG 0.323275 2.2:+59028535 None:intergenic
TGTGACAATCCTTTATCATT+TGG 0.329411 2.2:+59030495 None:intergenic
CTCTACTATTGCACATTCTA+TGG 0.347062 2.2:+59028013 None:intergenic
AAATGATCAAAACACTGAAC+TGG 0.347410 2.2:+59027153 None:intergenic
TTACCGTGATGTCGAGATTA+GGG 0.349365 2.2:+59030469 None:intergenic
ACTGTAGATGCTTCTGGTTA+TGG 0.350514 2.2:-59028470 MS.gene02020:CDS
CTGTAGATGCTTCTGGTTAT+GGG 0.368052 2.2:-59028469 MS.gene02020:CDS
GATGGTGGCGAACATGAAAA+TGG 0.368179 2.2:-59028971 MS.gene02020:CDS
ATGCCAACTGTAGATGCTTC+TGG 0.369452 2.2:-59028476 MS.gene02020:CDS
CTCTGTCCTCGTGCAAGCTT+CGG 0.376248 2.2:+59027790 None:intergenic
GCACGAGGACAGAGAGCTTT+GGG 0.378976 2.2:-59027781 MS.gene02020:CDS
CTTCTCTTTACTGTTACGTT+TGG 0.380146 2.2:+59028778 None:intergenic
TTTACCGTGATGTCGAGATT+AGG 0.383499 2.2:+59030468 None:intergenic
TTACTTCTGCCACCATAAAC+TGG 0.384916 2.2:-59028354 MS.gene02020:CDS
GGAAATTGGATTTGCTATTC+GGG 0.388562 2.2:-59028816 MS.gene02020:CDS
TTGGATTGATTCTAGTTCTA+GGG 0.391970 2.2:-59028270 MS.gene02020:CDS
AAATGCCTATCATTTCTTAC+CGG 0.393224 2.2:+59027387 None:intergenic
TGAGAGTAGTGGAATCTAAT+AGG 0.393466 2.2:-59028652 MS.gene02020:CDS
ATATTTCGAGCAGGGTTTGA+TGG 0.395255 2.2:+59026780 None:intergenic
TTATTGCATAATGTTGGGTC+AGG 0.399471 2.2:+59027829 None:intergenic
TGCACGAGGACAGAGAGCTT+TGG 0.400964 2.2:-59027782 MS.gene02020:CDS
GATTTCTTATTGCATAATGT+TGG 0.401141 2.2:+59027823 None:intergenic
TGGAATTTCTAATTACGAAA+AGG 0.404269 2.2:-59027727 MS.gene02020:CDS
TCAAACTCCACTCCCTCAAC+AGG 0.406663 2.2:+59028881 None:intergenic
CTTGCAATAGACAAATTCTA+TGG 0.410394 2.2:+59027973 None:intergenic
GCGTGTTCTCCAAATGATAA+AGG 0.419672 2.2:-59030504 MS.gene02020:CDS
ATTTCTTATTGCATAATGTT+GGG 0.422728 2.2:+59027824 None:intergenic
GGAATTTCTAATTACGAAAA+GGG 0.436213 2.2:-59027726 MS.gene02020:CDS
AATGTGTCCTTGGAGTAAAC+TGG 0.441443 2.2:+59027756 None:intergenic
GGTTTGTAACAATAGTTTCT+GGG 0.441868 2.2:+59028045 None:intergenic
CTCTTTCAGGATCAAATGAA+TGG 0.442050 2.2:+59028597 None:intergenic
ACTTCAAAGGCTACCTATGC+AGG 0.442185 2.2:-59027308 MS.gene02020:CDS
ATTCCACTAGTTGCATTTGT+TGG 0.443557 2.2:-59028182 MS.gene02020:CDS
AGATGAATTTGAAAGAGATT+GGG 0.444699 2.2:-59027856 MS.gene02020:CDS
GCAGATCATCCGTGGTTGTC+TGG 0.446361 2.2:-59027946 MS.gene02020:CDS
CTCCACTCCCTCAACAGGTT+GGG 0.449152 2.2:+59028886 None:intergenic
TGGAGAACACGCTGCATTGT+CGG 0.455406 2.2:+59030515 None:intergenic
TGTAATTGCTTTAACTTCAA+AGG 0.458366 2.2:-59027321 MS.gene02020:CDS
GATGCAGGTTGCAGCTGTTA+AGG 0.463308 2.2:-59026803 MS.gene02020:intron
AGAACATCAAGGGGAAGAAC+CGG 0.463849 2.2:-59027406 MS.gene02020:CDS
TAGTGGAATCTAATAGGTGG+AGG 0.476847 2.2:-59028646 MS.gene02020:CDS
ATAGCTATTATAATAGTTGT+AGG 0.483777 2.2:+59028844 None:intergenic
CTATTCGGGTTAAATCTTCG+TGG 0.484193 2.2:-59028802 MS.gene02020:CDS
CGCCCAACCTGTTGAGGGAG+TGG 0.485119 2.2:-59028888 MS.gene02020:CDS
TGCTCGAAATATGTGTTTGG+TGG 0.487448 2.2:-59026769 MS.gene02020:CDS
CCCTGCTCGAAATATGTGTT+TGG 0.491484 2.2:-59026772 MS.gene02020:CDS
ACATCATCACCACCTCATCT+TGG 0.493280 2.2:+59027490 None:intergenic
GTCTTGTGTTGTAGTTGTGA+AGG 0.496440 2.2:-59028746 MS.gene02020:CDS
TAGAGAGTTGCAACTCATAG+TGG 0.499235 2.2:+59027544 None:intergenic
CGTAGGAAAGAAACAAGAAC+AGG 0.499540 2.2:-59028695 MS.gene02020:CDS
AGACATTTGAAACGTATACT+TGG 0.502054 2.2:-59028106 MS.gene02020:CDS
ATCCTTAATTACAATGGTGT+GGG 0.502114 2.2:-59027273 MS.gene02020:CDS
ACTATTATAATAGCTATGCC+AGG 0.505200 2.2:-59028838 MS.gene02020:CDS
TTATCATAATTCATGTATGT+AGG 0.507183 2.2:+59026745 None:intergenic
AAGAACCGGTAAGAAATGAT+AGG 0.508332 2.2:-59027392 MS.gene02020:CDS
ACCAAACACATATTTCGAGC+AGG 0.509508 2.2:+59026771 None:intergenic
CACATAGGAGGATGGACAGT+GGG 0.512891 2.2:-59028559 MS.gene02020:CDS
CAAATTCTATGGTGAGCCCT+CGG 0.514324 2.2:+59027984 None:intergenic
ATTACAAATGCAACGTACTT+CGG 0.514793 2.2:+59027338 None:intergenic
TACTATTTCCAGATAGATGA+GGG 0.522528 2.2:-59029076 MS.gene02020:intron
TAAGCAACTACTTGAGGTTG+AGG 0.526880 2.2:-59029041 MS.gene02020:CDS
GGACAGTGGGGCCAAAAGAA+AGG 0.531952 2.2:-59028546 MS.gene02020:CDS
ATACTTGGTTATTTAGAGCA+TGG 0.532023 2.2:-59028091 MS.gene02020:CDS
GGGAGCTCAAATTCTAATGA+AGG 0.533763 2.2:-59028449 MS.gene02020:CDS
ATCAAAACACTGAACTGGGT+TGG 0.536498 2.2:+59027158 None:intergenic
AGACTACGCCCAACCTGTTG+AGG 0.540785 2.2:-59028894 MS.gene02020:CDS
AAATTCTATGGTGAGCCCTC+GGG 0.542802 2.2:+59027985 None:intergenic
TATCCTTAATTACAATGGTG+TGG 0.549164 2.2:-59027274 MS.gene02020:CDS
ACTCCACTCCCTCAACAGGT+TGG 0.553844 2.2:+59028885 None:intergenic
GACTACGCCCAACCTGTTGA+GGG 0.556672 2.2:-59028893 MS.gene02020:CDS
GTGATGGATCTGAATGATGA+TGG 0.557426 2.2:-59028311 MS.gene02020:CDS
AATAGCAAATCCAATTTCCC+TGG 0.562021 2.2:+59028820 None:intergenic
CCAAACACATATTTCGAGCA+GGG 0.562427 2.2:+59026772 None:intergenic
TATACAAGGCATGCCTGCAT+AGG 0.564010 2.2:+59027295 None:intergenic
GGAAGATGAGGCATTTCAGA+TGG 0.566330 2.2:-59027913 MS.gene02020:CDS
TATTGCATAATGTTGGGTCA+GGG 0.567535 2.2:+59027830 None:intergenic
ACATGGTAAAAGAACATCAA+GGG 0.568150 2.2:-59027416 MS.gene02020:CDS
CGAGTCTATAGTGACATACA+TGG 0.575473 2.2:-59027433 MS.gene02020:CDS
TTGTATATCCTTAATTACAA+TGG 0.575751 2.2:-59027279 MS.gene02020:CDS
CACATTCTATGGTCTTACAT+CGG 0.577150 2.2:+59028024 None:intergenic
CTCTAAATCACATAGGAGGA+TGG 0.578313 2.2:-59028567 MS.gene02020:CDS
GTTTGTAACAATAGTTTCTG+GGG 0.579296 2.2:+59028046 None:intergenic
TTTGTAACAATAGTTTCTGG+GGG 0.582883 2.2:+59028047 None:intergenic
TTACTATTTCCAGATAGATG+AGG 0.583204 2.2:-59029077 MS.gene02020:intron
GTGGCGAACATGAAAATGGA+AGG 0.585092 2.2:-59028967 MS.gene02020:CDS
ACTACTTGAGGTTGAGGACA+AGG 0.585298 2.2:-59029035 MS.gene02020:CDS
AATGATCAAAACACTGAACT+GGG 0.585970 2.2:+59027154 None:intergenic
ACAAAGTAAGCAACTACTTG+AGG 0.589391 2.2:-59029047 MS.gene02020:CDS
TCCTTAATTACAATGGTGTG+GGG 0.589480 2.2:-59027272 MS.gene02020:CDS
ATAAAGAAGCTAATAGTCGT+AGG 0.594056 2.2:-59028712 MS.gene02020:CDS
AGTCAATTGTTTGAGATCGA+TGG 0.594570 2.2:-59028989 MS.gene02020:CDS
TACATGGTAAAAGAACATCA+AGG 0.596297 2.2:-59027417 MS.gene02020:CDS
ACACCAACAAATGCAACTAG+TGG 0.596327 2.2:+59028179 None:intergenic
TTGGGCTCCAGTTTACTCCA+AGG 0.596913 2.2:-59027763 MS.gene02020:CDS
AAAACTCTAAATCACATAGG+AGG 0.599156 2.2:-59028571 MS.gene02020:CDS
GCTCGAAATATGTGTTTGGT+GGG 0.599382 2.2:-59026768 MS.gene02020:CDS
CGACTCTAGATGTTGAAGTA+CGG 0.600367 2.2:-59028517 MS.gene02020:CDS
GGAATCTAATAGGTGGAGGG+TGG 0.600823 2.2:-59028642 MS.gene02020:CDS
CTTGACAAAATATAATGACA+TGG 0.602201 2.2:+59027243 None:intergenic
CAATTGTTTGAGATCGATGG+TGG 0.603449 2.2:-59028986 MS.gene02020:CDS
CAAGTCTTGCAGATCATCCG+TGG 0.605006 2.2:-59027954 MS.gene02020:CDS
ATTCAATAAGTTCCAAGATG+AGG 0.606495 2.2:-59027502 MS.gene02020:CDS
CATAGAATGTGCAATAGTAG+AGG 0.608377 2.2:-59028012 MS.gene02020:CDS
TAACCAGAAGCATCTACAGT+TGG 0.609887 2.2:+59028473 None:intergenic
GGTATGCAAGTAGTTGAAGA+AGG 0.610848 2.2:-59027120 MS.gene02020:CDS
ACTATGACTGTCAACTCCCG+TGG 0.616006 2.2:+59028937 None:intergenic
GAGTAGTGGAATCTAATAGG+TGG 0.616272 2.2:-59028649 MS.gene02020:CDS
CAATAAGTTCCAAGATGAGG+TGG 0.616780 2.2:-59027499 MS.gene02020:CDS
AGCTCAAATTCTAATGAAGG+AGG 0.617343 2.2:-59028446 MS.gene02020:CDS
CGCAAAACTCTAAATCACAT+AGG 0.623536 2.2:-59028574 MS.gene02020:CDS
AATGGAAGGGAGGAAACCAC+GGG 0.626247 2.2:-59028953 MS.gene02020:CDS
GGTGCAGTTTCAGGAAGATG+AGG 0.635119 2.2:-59027925 MS.gene02020:CDS
AAATGGAAGGGAGGAAACCA+CGG 0.638573 2.2:-59028954 MS.gene02020:CDS
AAGCCCTAATCTCGACATCA+CGG 0.640632 2.2:-59030472 MS.gene02020:intron
CCTTAATTACAATGGTGTGG+GGG 0.643743 2.2:-59027271 MS.gene02020:CDS
ATTGTAATTAAGGATATACA+AGG 0.644132 2.2:+59027281 None:intergenic
CATGGTAAAAGAACATCAAG+GGG 0.645016 2.2:-59027415 MS.gene02020:CDS
CGTACTTCAACATCTAGAGT+CGG 0.648024 2.2:+59028518 None:intergenic
CTATTATAATAGCTATGCCA+GGG 0.648310 2.2:-59028837 MS.gene02020:CDS
TTTGTTGGTGTTAATCACCA+TGG 0.653331 2.2:-59028167 MS.gene02020:CDS
ACATTGTCAGAGCTGAGATG+TGG 0.654932 2.2:+59027189 None:intergenic
TAGGACTCACCAGTTTATGG+TGG 0.657453 2.2:+59028345 None:intergenic
AGTGGAATCTAATAGGTGGA+GGG 0.661358 2.2:-59028645 MS.gene02020:CDS
TTTGATCATTTGTACAAACG+TGG 0.664936 2.2:-59027141 MS.gene02020:CDS
AATGATTAGACTGAGAGTAG+TGG 0.669263 2.2:-59028663 MS.gene02020:CDS
TGGCGAACATGAAAATGGAA+GGG 0.678993 2.2:-59028966 MS.gene02020:CDS
CGAACATGAAAATGGAAGGG+AGG 0.681120 2.2:-59028963 MS.gene02020:CDS
TTGTAACAATAGTTTCTGGG+GGG 0.684006 2.2:+59028048 None:intergenic
CTACTTGAGGTTGAGGACAA+GGG 0.687690 2.2:-59029034 MS.gene02020:CDS
AAAGCTCCGAAGCTTGCACG+AGG 0.688357 2.2:-59027796 MS.gene02020:CDS
CTAGCAAAACAGATTGACCA+TGG 0.689075 2.2:+59028150 None:intergenic
GAAACTGCACCAGACAACCA+CGG 0.692031 2.2:+59027937 None:intergenic
TCACATAGGAGGATGGACAG+TGG 0.701404 2.2:-59028560 MS.gene02020:CDS
ACTATTTCCAGATAGATGAG+GGG 0.701880 2.2:-59029075 MS.gene02020:intron
ACATAGGAGGATGGACAGTG+GGG 0.705660 2.2:-59028558 MS.gene02020:CDS
CGTAACAGTAAAGAGAAGCG+TGG 0.709182 2.2:-59028773 MS.gene02020:CDS
TGAATGATGATGGACAATTG+AGG 0.749302 2.2:-59028301 MS.gene02020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTTTATTGATTAAC+AGG - chr2.2:59027379-59027398 MS.gene02020:CDS 10.0%
!!! ATATTGTTAACAATTTTAAA+GGG + chr2.2:59027640-59027659 None:intergenic 10.0%
!!! TATATTGTTAACAATTTTAA+AGG + chr2.2:59027641-59027660 None:intergenic 10.0%
!! AGTAATATAAAATTTCATGT+TGG - chr2.2:59028045-59028064 MS.gene02020:CDS 15.0%
!! ATAATCAGTGTTATAAATAA+AGG + chr2.2:59027834-59027853 None:intergenic 15.0%
!! ATTATTTCACATAATTACTT+TGG - chr2.2:59027356-59027375 MS.gene02020:CDS 15.0%
!! GTAATATAAAATTTCATGTT+GGG - chr2.2:59028046-59028065 MS.gene02020:CDS 15.0%
!! TGAAACTTATATTGTTAATA+TGG + chr2.2:59027701-59027720 None:intergenic 15.0%
!!! TATTGTTAACAATTTTAAAG+GGG + chr2.2:59027639-59027658 None:intergenic 15.0%
!!! TGAAATTTTATATTACTTAC+AGG + chr2.2:59028042-59028061 None:intergenic 15.0%
!! AAGATATAATGATAGTATAG+TGG - chr2.2:59027951-59027970 MS.gene02020:CDS 20.0%
!! AAGATTAAAAATTCAGAGTT+TGG + chr2.2:59026972-59026991 None:intergenic 20.0%
!! AGATATAATGATAGTATAGT+GGG - chr2.2:59027952-59027971 MS.gene02020:CDS 20.0%
!! ATAGCTATTATAATAGTTGT+AGG + chr2.2:59028413-59028432 None:intergenic 20.0%
!! ATTGTAATTAAGGATATACA+AGG + chr2.2:59029976-59029995 None:intergenic 20.0%
!! ATTTATAGAGTCTTTGAATA+AGG - chr2.2:59030196-59030215 MS.gene02020:intron 20.0%
!! ATTTCTTATTGCATAATGTT+GGG + chr2.2:59029433-59029452 None:intergenic 20.0%
!! CATGGTTACTTAAAATAAAA+GGG - chr2.2:59027237-59027256 MS.gene02020:CDS 20.0%
!! GTCATAACATAATATAATAG+CGG - chr2.2:59027762-59027781 MS.gene02020:CDS 20.0%
!! TCATGGTTACTTAAAATAAA+AGG - chr2.2:59027236-59027255 MS.gene02020:CDS 20.0%
!! TGTTATGACATTGATATTAT+TGG + chr2.2:59027751-59027770 None:intergenic 20.0%
!! TGTTTGCAAATAAATAGTTT+GGG + chr2.2:59026905-59026924 None:intergenic 20.0%
!! TTATCATAATTCATGTATGT+AGG + chr2.2:59030512-59030531 None:intergenic 20.0%
!! TTCAAAGAGAAAAAAAATTG+CGG - chr2.2:59027565-59027584 MS.gene02020:CDS 20.0%
!! TTGTATATCCTTAATTACAA+TGG - chr2.2:59029975-59029994 MS.gene02020:intron 20.0%
!! TTGTTTGCAAATAAATAGTT+TGG + chr2.2:59026906-59026925 None:intergenic 20.0%
!!! AAAAATTTGAAGTGATAAAC+AGG + chr2.2:59027286-59027305 None:intergenic 20.0%
!!! AAAATCATTATTTGGTTTCT+TGG - chr2.2:59030168-59030187 MS.gene02020:intron 20.0%
!!! AAGAAACCAAATAATGATTT+TGG + chr2.2:59030169-59030188 None:intergenic 20.0%
!!! AGAAACCAAATAATGATTTT+GGG + chr2.2:59030168-59030187 None:intergenic 20.0%
!!! AGAATAATTGTTTTTGAAGA+AGG - chr2.2:59027405-59027424 MS.gene02020:CDS 20.0%
!!! CAAATTCATCTAATGTTTTA+GGG + chr2.2:59029389-59029408 None:intergenic 20.0%
!!! GAATAATTGTTTTTGAAGAA+GGG - chr2.2:59027406-59027425 MS.gene02020:CDS 20.0%
!!! TCAAATTCATCTAATGTTTT+AGG + chr2.2:59029390-59029409 None:intergenic 20.0%
!!! TCTGTTTTTTAATTTCAAAG+TGG - chr2.2:59027122-59027141 MS.gene02020:CDS 20.0%
!!! TTTTAATGATAACACTGATA+GGG - chr2.2:59027592-59027611 MS.gene02020:CDS 20.0%
!!! TTTTTAATGATAACACTGAT+AGG - chr2.2:59027591-59027610 MS.gene02020:CDS 20.0%
! AACAATATAACCATGATTCA+AGG - chr2.2:59027652-59027671 MS.gene02020:CDS 25.0%
! AGAACAATTTATGTATCAGA+AGG + chr2.2:59030313-59030332 None:intergenic 25.0%
! AGATGAATTTGAAAGAGATT+GGG - chr2.2:59029398-59029417 MS.gene02020:intron 25.0%
! CTTGACAAAATATAATGACA+TGG + chr2.2:59030014-59030033 None:intergenic 25.0%
! GATATCCCAAAATCATTATT+TGG - chr2.2:59030160-59030179 MS.gene02020:intron 25.0%
! GATTTCTTATTGCATAATGT+TGG + chr2.2:59029434-59029453 None:intergenic 25.0%
! GGAATTTCTAATTACGAAAA+GGG - chr2.2:59029528-59029547 MS.gene02020:intron 25.0%
! GTGAAATAATCAATGTCATT+AGG + chr2.2:59027346-59027365 None:intergenic 25.0%
! TAGATGAATTTGAAAGAGAT+TGG - chr2.2:59029397-59029416 MS.gene02020:intron 25.0%
! TCCATCACATAGAAAAAATT+AGG + chr2.2:59028931-59028950 None:intergenic 25.0%
! TGGAATTTCTAATTACGAAA+AGG - chr2.2:59029527-59029546 MS.gene02020:intron 25.0%
! TTACTTAAAATAAAAGGGAG+TGG - chr2.2:59027242-59027261 MS.gene02020:CDS 25.0%
! TTTAATGATAACACTGATAG+GGG - chr2.2:59027593-59027612 MS.gene02020:CDS 25.0%
!! AAATAAATAGTTTGGGTTAG+AGG + chr2.2:59026898-59026917 None:intergenic 25.0%
!! ATAGAATGTGAATGTCTTTT+TGG - chr2.2:59030380-59030399 MS.gene02020:intron 25.0%
!! ATTATATTTTGTCAAGATGG+AGG - chr2.2:59030018-59030037 MS.gene02020:intron 25.0%
!! GTCATTATATTTTGTCAAGA+TGG - chr2.2:59030015-59030034 MS.gene02020:intron 25.0%
!! TGTAATTGCTTTAACTTCAA+AGG - chr2.2:59029933-59029952 MS.gene02020:intron 25.0%
!! TTTTGTTGAGAAAAGAAAAG+AGG - chr2.2:59026933-59026952 MS.gene02020:intron 25.0%
!!! ATCTATGATTCCCTTTTTTA+AGG - chr2.2:59029554-59029573 MS.gene02020:intron 25.0%
!!! TCCTAATTTTTTCTATGTGA+TGG - chr2.2:59028927-59028946 MS.gene02020:CDS 25.0%
!!! TCTATGATTCCCTTTTTTAA+GGG - chr2.2:59029555-59029574 MS.gene02020:intron 25.0%
AAAACTCTAAATCACATAGG+AGG - chr2.2:59028683-59028702 MS.gene02020:CDS 30.0%
AAACAAGTCATTAAGTTCCA+TGG + chr2.2:59026824-59026843 None:intergenic 30.0%
AAATGATCAAAACACTGAAC+TGG + chr2.2:59030104-59030123 None:intergenic 30.0%
AAATGCCTATCATTTCTTAC+CGG + chr2.2:59029870-59029889 None:intergenic 30.0%
AATATAACCATGATTCAAGG+CGG - chr2.2:59027655-59027674 MS.gene02020:CDS 30.0%
AATGATCAAAACACTGAACT+GGG + chr2.2:59030103-59030122 None:intergenic 30.0%
AATTTATGTATCAGAAGGCA+TGG + chr2.2:59030308-59030327 None:intergenic 30.0%
ACATGGTAAAAGAACATCAA+GGG - chr2.2:59029838-59029857 MS.gene02020:intron 30.0%
ACTATTATAATAGCTATGCC+AGG - chr2.2:59028416-59028435 MS.gene02020:CDS 30.0%
ATAAAGAAGCTAATAGTCGT+AGG - chr2.2:59028542-59028561 MS.gene02020:CDS 30.0%
ATCCTTAATTACAATGGTGT+GGG - chr2.2:59029981-59030000 MS.gene02020:intron 30.0%
ATGCTAATATGTTGTGCATA+AGG - chr2.2:59030262-59030281 MS.gene02020:intron 30.0%
ATTACAAATGCAACGTACTT+CGG + chr2.2:59029919-59029938 None:intergenic 30.0%
ATTCAATAAGTTCCAAGATG+AGG - chr2.2:59029752-59029771 MS.gene02020:intron 30.0%
CTATTATAATAGCTATGCCA+GGG - chr2.2:59028417-59028436 MS.gene02020:CDS 30.0%
CTTGCAATAGACAAATTCTA+TGG + chr2.2:59029284-59029303 None:intergenic 30.0%
GGTTTGTAACAATAGTTTCT+GGG + chr2.2:59029212-59029231 None:intergenic 30.0%
GTAAATCTTCATCTTAACCA+TGG - chr2.2:59026804-59026823 MS.gene02020:intron 30.0%
GTTTGTAACAATAGTTTCTG+GGG + chr2.2:59029211-59029230 None:intergenic 30.0%
TACATGGTAAAAGAACATCA+AGG - chr2.2:59029837-59029856 MS.gene02020:intron 30.0%
TACTATTTCCAGATAGATGA+GGG - chr2.2:59028178-59028197 MS.gene02020:CDS 30.0%
TATCCTTAATTACAATGGTG+TGG - chr2.2:59029980-59029999 MS.gene02020:intron 30.0%
TGTGACAATCCTTTATCATT+TGG + chr2.2:59026762-59026781 None:intergenic 30.0%
TTACTATTTCCAGATAGATG+AGG - chr2.2:59028177-59028196 MS.gene02020:CDS 30.0%
TTTGATCATTTGTACAAACG+TGG - chr2.2:59030113-59030132 MS.gene02020:intron 30.0%
! AAACAGTGTTGTTAAATAGC+AGG - chr2.2:59027450-59027469 MS.gene02020:CDS 30.0%
! AGACATTTGAAACGTATACT+TGG - chr2.2:59029148-59029167 MS.gene02020:intron 30.0%
! ATACTTGGTTATTTAGAGCA+TGG - chr2.2:59029163-59029182 MS.gene02020:intron 30.0%
! ATTTATGTATCAGAAGGCAT+GGG + chr2.2:59030307-59030326 None:intergenic 30.0%
! CTTTGTTTATGTTAGCCATT+AGG - chr2.2:59027308-59027327 MS.gene02020:CDS 30.0%
! GACAATTGAGGAACATATTT+TGG - chr2.2:59028965-59028984 MS.gene02020:CDS 30.0%
! TAATGATAGTATAGTGGGAA+AGG - chr2.2:59027957-59027976 MS.gene02020:CDS 30.0%
! TTTGTAACAATAGTTTCTGG+GGG + chr2.2:59029210-59029229 None:intergenic 30.0%
!! TTGGATTGATTCTAGTTCTA+GGG - chr2.2:59028984-59029003 MS.gene02020:CDS 30.0%
!! TTTGGATTGATTCTAGTTCT+AGG - chr2.2:59028983-59029002 MS.gene02020:CDS 30.0%
!!! ATATAGTTATTTTGGTGACG+TGG - chr2.2:59029011-59029030 MS.gene02020:CDS 30.0%
AAGAACCGGTAAGAAATGAT+AGG - chr2.2:59029862-59029881 MS.gene02020:intron 35.0%
AATAGCAAATCCAATTTCCC+TGG + chr2.2:59028437-59028456 None:intergenic 35.0%
AATGATTAGACTGAGAGTAG+TGG - chr2.2:59028591-59028610 MS.gene02020:CDS 35.0%
AATGCATAAACCATAGTGGT+TGG - chr2.2:59030427-59030446 MS.gene02020:intron 35.0%
AATTAGGACTCACCAGTTTA+TGG + chr2.2:59028915-59028934 None:intergenic 35.0%
AATTTCATGTTGGGAACTGT+AGG - chr2.2:59028055-59028074 MS.gene02020:CDS 35.0%
ACAAAGTAAGCAACTACTTG+AGG - chr2.2:59028207-59028226 MS.gene02020:CDS 35.0%
ACTATTTCCAGATAGATGAG+GGG - chr2.2:59028179-59028198 MS.gene02020:CDS 35.0%
AGAGAATAAAGAGACGACTA+GGG - chr2.2:59027723-59027742 MS.gene02020:CDS 35.0%
AGCTCAAATTCTAATGAAGG+AGG - chr2.2:59028808-59028827 MS.gene02020:CDS 35.0%
AGGAGACTGAAGCTAAAAAA+AGG - chr2.2:59028856-59028875 MS.gene02020:CDS 35.0%
ATACAGGATCATGATTGTGT+AGG - chr2.2:59027993-59028012 MS.gene02020:CDS 35.0%
ATCATGAGGAACATCCATAA+CGG + chr2.2:59027885-59027904 None:intergenic 35.0%
ATGCACATGACCCTTAAAAA+AGG + chr2.2:59029568-59029587 None:intergenic 35.0%
ATTCCACTAGTTGCATTTGT+TGG - chr2.2:59029072-59029091 MS.gene02020:intron 35.0%
CATAGAATGTGCAATAGTAG+AGG - chr2.2:59029242-59029261 MS.gene02020:intron 35.0%
CATGGGTGAACAAAAATGAA+TGG + chr2.2:59030290-59030309 None:intergenic 35.0%
CATGGTAAAAGAACATCAAG+GGG - chr2.2:59029839-59029858 MS.gene02020:intron 35.0%
CATTTCCAACTATGACATTG+TGG - chr2.2:59027042-59027061 MS.gene02020:intron 35.0%
CGCAAAACTCTAAATCACAT+AGG - chr2.2:59028680-59028699 MS.gene02020:CDS 35.0%
CGGTTTGTAACAATAGTTTC+TGG + chr2.2:59029213-59029232 None:intergenic 35.0%
CGTTAGTGTGAGATAAATGA+GGG - chr2.2:59027188-59027207 MS.gene02020:CDS 35.0%
CTCCAAGTTCTGTGAAAAAA+TGG - chr2.2:59027147-59027166 MS.gene02020:CDS 35.0%
CTCTACTATTGCACATTCTA+TGG + chr2.2:59029244-59029263 None:intergenic 35.0%
CTCTTTCAGGATCAAATGAA+TGG + chr2.2:59028660-59028679 None:intergenic 35.0%
CTTCTCTTTACTGTTACGTT+TGG + chr2.2:59028479-59028498 None:intergenic 35.0%
GATCAATGCATAAACCATAG+TGG - chr2.2:59030423-59030442 MS.gene02020:intron 35.0%
GTTGGAAATGCAAATCAATC+AGG + chr2.2:59027032-59027051 None:intergenic 35.0%
TACAGGATCATGATTGTGTA+GGG - chr2.2:59027994-59028013 MS.gene02020:CDS 35.0%
TATTGCATAATGTTGGGTCA+GGG + chr2.2:59029427-59029446 None:intergenic 35.0%
TCCTTAATTACAATGGTGTG+GGG - chr2.2:59029982-59030001 MS.gene02020:intron 35.0%
TCGTTAGTGTGAGATAAATG+AGG - chr2.2:59027187-59027206 MS.gene02020:CDS 35.0%
TGCACATGACCCTTAAAAAA+GGG + chr2.2:59029567-59029586 None:intergenic 35.0%
TTATTGCATAATGTTGGGTC+AGG + chr2.2:59029428-59029447 None:intergenic 35.0%
! AACTGAAGCTCTTGATGATT+TGG - chr2.2:59029650-59029669 MS.gene02020:intron 35.0%
! AGGGCTGCATATAGTTATTT+TGG - chr2.2:59029003-59029022 MS.gene02020:CDS 35.0%
! AGTCAATTGTTTGAGATCGA+TGG - chr2.2:59028265-59028284 MS.gene02020:CDS 35.0%
! CACATTCTATGGTCTTACAT+CGG + chr2.2:59029233-59029252 None:intergenic 35.0%
! TATAGTGGGAAAGGTGAAAA+CGG - chr2.2:59027966-59027985 MS.gene02020:CDS 35.0%
! TGAGAGTAGTGGAATCTAAT+AGG - chr2.2:59028602-59028621 MS.gene02020:CDS 35.0%
! TTATGCGTTTTGCGAATTTC+TGG - chr2.2:59027787-59027806 MS.gene02020:CDS 35.0%
! TTGTAACAATAGTTTCTGGG+GGG + chr2.2:59029209-59029228 None:intergenic 35.0%
!! GGAAATTGGATTTGCTATTC+GGG - chr2.2:59028438-59028457 MS.gene02020:CDS 35.0%
!! GGGAAATTGGATTTGCTATT+CGG - chr2.2:59028437-59028456 MS.gene02020:CDS 35.0%
!! TGAATGATGATGGACAATTG+AGG - chr2.2:59028953-59028972 MS.gene02020:CDS 35.0%
!! TTTGTTGGTGTTAATCACCA+TGG - chr2.2:59029087-59029106 MS.gene02020:intron 35.0%
!!! CACCATTTTTTCACAGAACT+TGG + chr2.2:59027152-59027171 None:intergenic 35.0%
!!! TTTTTTTAGCTTCAGTCTCC+TGG + chr2.2:59028857-59028876 None:intergenic 35.0%
AAATAAAAGGGAGTGGTGCA+TGG - chr2.2:59027249-59027268 MS.gene02020:CDS 40.0%
AACCATGACTGCAAGACAAT+TGG + chr2.2:59027224-59027243 None:intergenic 40.0%
AACTATATCTTCCACACGAG+TGG + chr2.2:59027917-59027936 None:intergenic 40.0%
AATAGCTATGCCAGGGAAAT+TGG - chr2.2:59028424-59028443 MS.gene02020:CDS 40.0%
AATGTGTCCTTGGAGTAAAC+TGG + chr2.2:59029501-59029520 None:intergenic 40.0%
ACACCAACAAATGCAACTAG+TGG + chr2.2:59029078-59029097 None:intergenic 40.0%
ACCAAACACATATTTCGAGC+AGG + chr2.2:59030486-59030505 None:intergenic 40.0%
ACTTGCCACAATGTCATAGT+TGG + chr2.2:59027050-59027069 None:intergenic 40.0%
AGTGGAATCTAATAGGTGGA+GGG - chr2.2:59028609-59028628 MS.gene02020:CDS 40.0%
ATATTTCGAGCAGGGTTTGA+TGG + chr2.2:59030477-59030496 None:intergenic 40.0%
ATCAAAACACTGAACTGGGT+TGG + chr2.2:59030099-59030118 None:intergenic 40.0%
ATCACAACCAAGCACAAAGA+TGG + chr2.2:59028081-59028100 None:intergenic 40.0%
ATGGATGTTCCTCATGATCT+TGG - chr2.2:59027887-59027906 MS.gene02020:CDS 40.0%
ATTTGATCCGCCTTGAATCA+TGG + chr2.2:59027665-59027684 None:intergenic 40.0%
CAATAAGTTCCAAGATGAGG+TGG - chr2.2:59029755-59029774 MS.gene02020:intron 40.0%
CAGAGAATAAAGAGACGACT+AGG - chr2.2:59027722-59027741 MS.gene02020:CDS 40.0%
CCAAACACATATTTCGAGCA+GGG + chr2.2:59030485-59030504 None:intergenic 40.0%
CCTTAATTACAATGGTGTGG+GGG - chr2.2:59029983-59030002 MS.gene02020:intron 40.0%
CGACTCTAGATGTTGAAGTA+CGG - chr2.2:59028737-59028756 MS.gene02020:CDS 40.0%
CGAGTCTATAGTGACATACA+TGG - chr2.2:59029821-59029840 MS.gene02020:intron 40.0%
CGTAGGAAAGAAACAAGAAC+AGG - chr2.2:59028559-59028578 MS.gene02020:CDS 40.0%
CTAGCAAAACAGATTGACCA+TGG + chr2.2:59029107-59029126 None:intergenic 40.0%
CTCTAAATCACATAGGAGGA+TGG - chr2.2:59028687-59028706 MS.gene02020:CDS 40.0%
GCGTGTTCTCCAAATGATAA+AGG - chr2.2:59026750-59026769 MS.gene02020:CDS 40.0%
GCTCGAAATATGTGTTTGGT+GGG - chr2.2:59030486-59030505 MS.gene02020:CDS 40.0%
GGGAGCTCAAATTCTAATGA+AGG - chr2.2:59028805-59028824 MS.gene02020:CDS 40.0%
GGTATGCAAGTAGTTGAAGA+AGG - chr2.2:59030134-59030153 MS.gene02020:intron 40.0%
GTCTTGTGTTGTAGTTGTGA+AGG - chr2.2:59028508-59028527 MS.gene02020:CDS 40.0%
GTTAGCCATTAGGATGTCAT+AGG - chr2.2:59027318-59027337 MS.gene02020:CDS 40.0%
TAAGCAACTACTTGAGGTTG+AGG - chr2.2:59028213-59028232 MS.gene02020:CDS 40.0%
TAGAGAGTTGCAACTCATAG+TGG + chr2.2:59029713-59029732 None:intergenic 40.0%
TAGTGGAATCTAATAGGTGG+AGG - chr2.2:59028608-59028627 MS.gene02020:CDS 40.0%
TCCAGCAAAAAATGTGTCCT+TGG + chr2.2:59029511-59029530 None:intergenic 40.0%
TCGTGTGGAAGATATAGTTG+TGG - chr2.2:59027918-59027937 MS.gene02020:CDS 40.0%
TGCTATGCTGAAACTGTATG+CGG - chr2.2:59027497-59027516 MS.gene02020:CDS 40.0%
TGCTCGAAATATGTGTTTGG+TGG - chr2.2:59030485-59030504 MS.gene02020:CDS 40.0%
TGGCGAACATGAAAATGGAA+GGG - chr2.2:59028288-59028307 MS.gene02020:CDS 40.0%
TTACCGTGATGTCGAGATTA+GGG + chr2.2:59026788-59026807 None:intergenic 40.0%
TTACTTCTGCCACCATAAAC+TGG - chr2.2:59028900-59028919 MS.gene02020:CDS 40.0%
TTTACCGTGATGTCGAGATT+AGG + chr2.2:59026789-59026808 None:intergenic 40.0%
! AATAGTAGAGGTTTTCCCGA+GGG - chr2.2:59029254-59029273 MS.gene02020:intron 40.0%
! ACTGTAGATGCTTCTGGTTA+TGG - chr2.2:59028784-59028803 MS.gene02020:CDS 40.0%
! ATAGTGGGAAAGGTGAAAAC+GGG - chr2.2:59027967-59027986 MS.gene02020:CDS 40.0%
! CAATCTGTTTTGCTAGGATG+TGG - chr2.2:59029111-59029130 MS.gene02020:intron 40.0%
! CAATTGTTTGAGATCGATGG+TGG - chr2.2:59028268-59028287 MS.gene02020:CDS 40.0%
! CATGGTCAATCTGTTTTGCT+AGG - chr2.2:59029105-59029124 MS.gene02020:intron 40.0%
! CGTACTTCAACATCTAGAGT+CGG + chr2.2:59028739-59028758 None:intergenic 40.0%
! CTATTCGGGTTAAATCTTCG+TGG - chr2.2:59028452-59028471 MS.gene02020:CDS 40.0%
! CTGTAGATGCTTCTGGTTAT+GGG - chr2.2:59028785-59028804 MS.gene02020:CDS 40.0%
! GAGTAGTGGAATCTAATAGG+TGG - chr2.2:59028605-59028624 MS.gene02020:CDS 40.0%
! GCAACAACGTTAAGTTTTCC+AGG - chr2.2:59028836-59028855 MS.gene02020:CDS 40.0%
! TGTGCTTTTCAGTCTGATGT+TGG - chr2.2:59026849-59026868 MS.gene02020:intron 40.0%
! TTAGAGTTTTGCGCTCTTTC+AGG + chr2.2:59028673-59028692 None:intergenic 40.0%
! TTTTCAGTCTGATGTTGGAC+TGG - chr2.2:59026854-59026873 MS.gene02020:intron 40.0%
!! GTGATGGATCTGAATGATGA+TGG - chr2.2:59028943-59028962 MS.gene02020:CDS 40.0%
!! TAACCAGAAGCATCTACAGT+TGG + chr2.2:59028784-59028803 None:intergenic 40.0%
!!! TCCAAGGACACATTTTTTGC+TGG - chr2.2:59029507-59029526 MS.gene02020:intron 40.0%
!!! TGCGAATTTCTGGTGTTTTG+CGG - chr2.2:59027797-59027816 MS.gene02020:CDS 40.0%
AAATGGAAGGGAGGAAACCA+CGG - chr2.2:59028300-59028319 MS.gene02020:CDS 45.0%
AAATTCTATGGTGAGCCCTC+GGG + chr2.2:59029272-59029291 None:intergenic 45.0%
AAGCCCTAATCTCGACATCA+CGG - chr2.2:59026782-59026801 MS.gene02020:CDS 45.0%
ACATCATCACCACCTCATCT+TGG + chr2.2:59029767-59029786 None:intergenic 45.0%
ACATTGTCAGAGCTGAGATG+TGG + chr2.2:59030068-59030087 None:intergenic 45.0%
ACTACTTGAGGTTGAGGACA+AGG - chr2.2:59028219-59028238 MS.gene02020:CDS 45.0%
ACTTCAAAGGCTACCTATGC+AGG - chr2.2:59029946-59029965 MS.gene02020:intron 45.0%
AGAACATCAAGGGGAAGAAC+CGG - chr2.2:59029848-59029867 MS.gene02020:intron 45.0%
AGGCACCTATGACATCCTAA+TGG + chr2.2:59027326-59027345 None:intergenic 45.0%
AGGTGAAAACGGGTAGATAC+AGG - chr2.2:59027977-59027996 MS.gene02020:CDS 45.0%
ATATCCCCTATTGCCGATCT+GGG + chr2.2:59027537-59027556 None:intergenic 45.0%
ATGCCAACTGTAGATGCTTC+TGG - chr2.2:59028778-59028797 MS.gene02020:CDS 45.0%
CAAATTCTATGGTGAGCCCT+CGG + chr2.2:59029273-59029292 None:intergenic 45.0%
CACCAATTGTCTTGCAGTCA+TGG - chr2.2:59027219-59027238 MS.gene02020:CDS 45.0%
CCCCCACACCATTGTAATTA+AGG + chr2.2:59029986-59030005 None:intergenic 45.0%
CCCTGCTCGAAATATGTGTT+TGG - chr2.2:59030482-59030501 MS.gene02020:CDS 45.0%
CGAACATGAAAATGGAAGGG+AGG - chr2.2:59028291-59028310 MS.gene02020:CDS 45.0%
CGTAACAGTAAAGAGAAGCG+TGG - chr2.2:59028481-59028500 MS.gene02020:CDS 45.0%
CTACTTGAGGTTGAGGACAA+GGG - chr2.2:59028220-59028239 MS.gene02020:CDS 45.0%
GGTTCGTTGTGTAACAGACA+AGG - chr2.2:59030217-59030236 MS.gene02020:intron 45.0%
GTGGCGAACATGAAAATGGA+AGG - chr2.2:59028287-59028306 MS.gene02020:CDS 45.0%
TAGGACTCACCAGTTTATGG+TGG + chr2.2:59028912-59028931 None:intergenic 45.0%
TATACAAGGCATGCCTGCAT+AGG + chr2.2:59029962-59029981 None:intergenic 45.0%
TATATCCCCTATTGCCGATC+TGG + chr2.2:59027538-59027557 None:intergenic 45.0%
TGCTATTCCCAATCCCAGAT+CGG - chr2.2:59027521-59027540 MS.gene02020:CDS 45.0%
! CAATAGTAGAGGTTTTCCCG+AGG - chr2.2:59029253-59029272 MS.gene02020:intron 45.0%
! GATGGTGGCGAACATGAAAA+TGG - chr2.2:59028283-59028302 MS.gene02020:CDS 45.0%
! GGAAGATGAGGCATTTCAGA+TGG - chr2.2:59029341-59029360 MS.gene02020:intron 45.0%
!! AGTCGGCTCAACCTTTCTTT+TGG + chr2.2:59028722-59028741 None:intergenic 45.0%
!!! TGGCATCTTGTTTTCAGCTG+TGG + chr2.2:59029693-59029712 None:intergenic 45.0%
AATGGAAGGGAGGAAACCAC+GGG - chr2.2:59028301-59028320 MS.gene02020:CDS 50.0%
ACATAGGAGGATGGACAGTG+GGG - chr2.2:59028696-59028715 MS.gene02020:CDS 50.0%
ACCTGCATCACCAACCACTA+TGG + chr2.2:59030440-59030459 None:intergenic 50.0%
ACTATGACTGTCAACTCCCG+TGG + chr2.2:59028320-59028339 None:intergenic 50.0%
AGATCGATGCCAGTCCGTTA+TGG - chr2.2:59027868-59027887 MS.gene02020:CDS 50.0%
AGTGGACAGCCAAGATCATG+AGG + chr2.2:59027899-59027918 None:intergenic 50.0%
CAAGTCTTGCAGATCATCCG+TGG - chr2.2:59029300-59029319 MS.gene02020:intron 50.0%
CAATCCCAGATCGGCAATAG+GGG - chr2.2:59027530-59027549 MS.gene02020:CDS 50.0%
CACATAGGAGGATGGACAGT+GGG - chr2.2:59028695-59028714 MS.gene02020:CDS 50.0%
CCAATCCCAGATCGGCAATA+GGG - chr2.2:59027529-59027548 MS.gene02020:CDS 50.0%
CTGTAGGCCATCTTTGTGCT+TGG - chr2.2:59028071-59028090 MS.gene02020:CDS 50.0%
GAAACTGCACCAGACAACCA+CGG + chr2.2:59029320-59029339 None:intergenic 50.0%
GATGCAGGTTGCAGCTGTTA+AGG - chr2.2:59030451-59030470 MS.gene02020:intron 50.0%
GGAATCTAATAGGTGGAGGG+TGG - chr2.2:59028612-59028631 MS.gene02020:CDS 50.0%
GGTGCAGTTTCAGGAAGATG+AGG - chr2.2:59029329-59029348 MS.gene02020:intron 50.0%
GTAGAGACCCCTCATCTATC+TGG + chr2.2:59028189-59028208 None:intergenic 50.0%
TCAAACTCCACTCCCTCAAC+AGG + chr2.2:59028376-59028395 None:intergenic 50.0%
TCACATAGGAGGATGGACAG+TGG - chr2.2:59028694-59028713 MS.gene02020:CDS 50.0%
TGGAGAACACGCTGCATTGT+CGG + chr2.2:59026742-59026761 None:intergenic 50.0%
TGGTTGTCTGGTGCAGTTTC+AGG - chr2.2:59029320-59029339 MS.gene02020:intron 50.0%
TTGGGCTCCAGTTTACTCCA+AGG - chr2.2:59029491-59029510 MS.gene02020:intron 50.0%
! CCTATTGCCGATCTGGGATT+GGG + chr2.2:59027531-59027550 None:intergenic 50.0%
!! ACCATAGTGGTTGGTGATGC+AGG - chr2.2:59030436-59030455 MS.gene02020:intron 50.0%
!! GAGGAACATCCATAACGGAC+TGG + chr2.2:59027880-59027899 None:intergenic 50.0%
AAAGCTCCGAAGCTTGCACG+AGG - chr2.2:59029458-59029477 MS.gene02020:intron 55.0%
ACTCCACTCCCTCAACAGGT+TGG + chr2.2:59028372-59028391 None:intergenic 55.0%
AGACTACGCCCAACCTGTTG+AGG - chr2.2:59028360-59028379 MS.gene02020:CDS 55.0%
ATCTTGGCTGTCCACTCGTG+TGG - chr2.2:59027903-59027922 MS.gene02020:CDS 55.0%
CCCAATCCCAGATCGGCAAT+AGG - chr2.2:59027528-59027547 MS.gene02020:CDS 55.0%
CTCCACTCCCTCAACAGGTT+GGG + chr2.2:59028371-59028390 None:intergenic 55.0%
CTCTGTCCTCGTGCAAGCTT+CGG + chr2.2:59029467-59029486 None:intergenic 55.0%
GACTACGCCCAACCTGTTGA+GGG - chr2.2:59028361-59028380 MS.gene02020:CDS 55.0%
GCACGAGGACAGAGAGCTTT+GGG - chr2.2:59029473-59029492 MS.gene02020:intron 55.0%
GCAGATCATCCGTGGTTGTC+TGG - chr2.2:59029308-59029327 MS.gene02020:intron 55.0%
GGACAGTGGGGCCAAAAGAA+AGG - chr2.2:59028708-59028727 MS.gene02020:CDS 55.0%
TGCACGAGGACAGAGAGCTT+TGG - chr2.2:59029472-59029491 MS.gene02020:intron 55.0%
! CCCTATTGCCGATCTGGGAT+TGG + chr2.2:59027532-59027551 None:intergenic 55.0%
CGCCCAACCTGTTGAGGGAG+TGG - chr2.2:59028366-59028385 MS.gene02020:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 59026735 59030541 59026735 ID=MS.gene02020
chr2.2 mRNA 59026735 59030541 59026735 ID=MS.gene02020.t1;Parent=MS.gene02020
chr2.2 exon 59030473 59030541 59030473 ID=MS.gene02020.t1.exon1;Parent=MS.gene02020.t1
chr2.2 CDS 59030473 59030541 59030473 ID=cds.MS.gene02020.t1;Parent=MS.gene02020.t1
chr2.2 exon 59027059 59029086 59027059 ID=MS.gene02020.t1.exon2;Parent=MS.gene02020.t1
chr2.2 CDS 59027059 59029086 59027059 ID=cds.MS.gene02020.t1;Parent=MS.gene02020.t1
chr2.2 exon 59026735 59026818 59026735 ID=MS.gene02020.t1.exon3;Parent=MS.gene02020.t1
chr2.2 CDS 59026735 59026818 59026735 ID=cds.MS.gene02020.t1;Parent=MS.gene02020.t1
Gene Sequence

>MS.gene02020

ATGACCGACAATGCAGCGTGTTCTCCAAATGATAAAGGATTGTCACAAAGCCCTAATCTCGACATCACGGTAAATCTTCATCTTAACCATGGAACTTAATGACTTGTTTTATGCTGTGCTTTTCAGTCTGATGTTGGACTGGATCTTATTTGTCTATCTGCCTCTAACCCAAACTATTTATTTGCAAACAACTCACGCTTTTGTTGAGAAAAGAAAAGAGGAATCAAATTAACGCCAAACTCTGAATTTTTAATCTTTTAAGACTTTAGTGTACTGTATAGTTTTGCTAAATTTCCTGATTGATTTGCATTTCCAACTATGACATTGTGGCAAGTCTTGAAGAACACGACACTCGATCTTTCTCGTATTGAGATACTTTTGTAATTTTCTGTTTTTTAATTTCAAAGTGGTTCTCCAAGTTCTGTGAAAAAATGGTGCAATGTACAATTTTTTCGTTAGTGTGAGATAAATGAGGGAAGAAGTGCACCAATTGTCTTGCAGTCATGGTTACTTAAAATAAAAGGGAGTGGTGCATGGATTTTAATTATCCTGTTTATCACTTCAAATTTTTGTCTTTGTTTATGTTAGCCATTAGGATGTCATAGGTGCCTAATGACATTGATTATTTCACATAATTACTTTGGAATATTTTTTATTGATTAACAGGCATAGAATAATTGTTTTTGAAGAAGGGCATAGAATAATTGTTGATTATAAACAGTGTTGTTAAATAGCAGGATGTGAACAAAAAACTATGAAAACTGCTATGCTGAAACTGTATGCGGCTGCTATTCCCAATCCCAGATCGGCAATAGGGGATATAGCATTAATTCAAAGAGAAAAAAAATTGCGGTTTTTTTTAATGATAACACTGATAGGGGAGCATGTGAGCTGCACATTACCCCTTTAAAATTGTTAACAATATAACCATGATTCAAGGCGGATCAAATCATGCTCTTGATACCATATTAACAATATAAGTTTCAACAGAGAATAAAGAGACGACTAGGGTTCCAATAATATCAATGTCATAACATAATATAATAGCGGATTTATGCGTTTTGCGAATTTCTGGTGTTTTGCGGATTTCTGTGATCCTTTATTTATAACACTGATTATAAATCATTTATCTGAGATCGATGCCAGTCCGTTATGGATGTTCCTCATGATCTTGGCTGTCCACTCGTGTGGAAGATATAGTTGTGGTATTGCTTCAAAGATATAATGATAGTATAGTGGGAAAGGTGAAAACGGGTAGATACAGGATCATGATTGTGTAGGGTGAAAATAAAAGAAACTTCACACCTGTAAGTAATATAAAATTTCATGTTGGGAACTGTAGGCCATCTTTGTGCTTGGTTGTGATATTTCAAGTTCTGTTGTCAAATTCAATGCATACTCAAATTTCTTAGACTGTGTTTAGAATTTGTTTTTTTATTTCATTACTATTTCCAGATAGATGAGGGGTCTCTACAAAGTAAGCAACTACTTGAGGTTGAGGACAAGGGCAACGAGCTTGAGAATGACTGTAGTCAATTGTTTGAGATCGATGGTGGCGAACATGAAAATGGAAGGGAGGAAACCACGGGAGTTGACAGTCATAGTGAAGAATCTCAAAGAACAAAAGACTACGCCCAACCTGTTGAGGGAGTGGAGTTTGAAACTTATGATGATGCCTACAACTATTATAATAGCTATGCCAGGGAAATTGGATTTGCTATTCGGGTTAAATCTTCGTGGACCAAACGTAACAGTAAAGAGAAGCGTGGTGCTGTCTTGTGTTGTAGTTGTGAAGGTTTCAAAACAAATAAAGAAGCTAATAGTCGTAGGAAAGAAACAAGAACAGGCTGTCTAGCAATGATTAGACTGAGAGTAGTGGAATCTAATAGGTGGAGGGTGGATGAAGTCAAGCTTGAGCATAACCATTCATTTGATCCTGAAAGAGCGCAAAACTCTAAATCACATAGGAGGATGGACAGTGGGGCCAAAAGAAAGGTTGAGCCGACTCTAGATGTTGAAGTACGGACAATCAAGTTATATCGAATGCCAACTGTAGATGCTTCTGGTTATGGGAGCTCAAATTCTAATGAAGGAGGAACTAGCAACAACGTTAAGTTTTCCAGGAGACTGAAGCTAAAAAAAGGTGATGCAGAACTTATTTCAAATTACTTCTGCCACCATAAACTGGTGAGTCCTAATTTTTTCTATGTGATGGATCTGAATGATGATGGACAATTGAGGAACATATTTTGGATTGATTCTAGTTCTAGGGCTGCATATAGTTATTTTGGTGACGTGGTTGCATTTGACTCAACATATTTGTCAAACAATTATGAGATTCCACTAGTTGCATTTGTTGGTGTTAATCACCATGGTCAATCTGTTTTGCTAGGATGTGGTCTGCTTGCAGATGAGACATTTGAAACGTATACTTGGTTATTTAGAGCATGGCTTTCTTGTATGTCTAGTCGCCCCCCAGAAACTATTGTTACAAACCGATGTAAGACCATAGAATGTGCAATAGTAGAGGTTTTCCCGAGGGCTCACCATAGAATTTGTCTATTGCAAGTCTTGCAGATCATCCGTGGTTGTCTGGTGCAGTTTCAGGAAGATGAGGCATTTCAGATGGCACTGACAAGAGTGATATATGACCCTAAAACATTAGATGAATTTGAAAGAGATTGGGATTCCCTGACCCAACATTATGCAATAAGAAATCATGCAAAGCTCCGAAGCTTGCACGAGGACAGAGAGCTTTGGGCTCCAGTTTACTCCAAGGACACATTTTTTGCTGGAATTTCTAATTACGAAAAGGGTGAATCTATGATTCCCTTTTTTAAGGGTCATGTGCATCAACAAACTTCTTTGAAAGAATTTTTTGAGATTTATGAATTAGTTCAACAAAAAAAGCAAAAAACTGAAGCTCTTGATGATTTGGAGTCACAAAATTCAAGTCCACAGCTGAAAACAAGATGCCACTATGAGTTGCAACTCTCTAAATTGTACACGAATGCAATATTCAATAAGTTCCAAGATGAGGTGGTGATGATGTCTTCTTGTTTCAGCGTCTCACAAATTCAAACAAACGAGTCTATAGTGACATACATGGTAAAAGAACATCAAGGGGAAGAACCGGTAAGAAATGATAGGCATTTTGAAGTCATGTATGATAAAGCATTAACCGAAGTACGTTGCATTTGTAATTGCTTTAACTTCAAAGGCTACCTATGCAGGCATGCCTTGTATATCCTTAATTACAATGGTGTGGGGGAAATTCCATGTCATTATATTTTGTCAAGATGGAGGAAAGATTTTAAGCGATTGTATGTACCACATCTCAGCTCTGACAATGTTGATATTACCAACCCAGTTCAGTGTTTTGATCATTTGTACAAACGTGGTATGCAAGTAGTTGAAGAAGGCACGATATCCCAAAATCATTATTTGGTTTCTTGGCAGACATTTATAGAGTCTTTGAATAAGGTTCGTTGTGTAACAGACAAGGTTGAATAAATAAATCAGAAATAATGCTAATATGTTGTGCATAAGGTACCATTCATTTTTGTTCACCCATGCCTTCTGATACATAAATTGTTCTCTTATAGTTATAGTTTATGTTAAAAGTTTGCAGAATTATGTGAAAGCATAGAATGTGAATGTCTTTTTGGTTCGTTTACTAAATCAATTTGATCAATGCATAAACCATAGTGGTTGGTGATGCAGGTTGCAGCTGTTAAGGCCATCAAACCCTGCTCGAAATATGTGTTTGGTGGGCCTACATACATGAATTATGATAAAGAGAAATAG
Protein sequence

>MS.gene02020.t1

MTDNAACSPNDKGLSQSPNLDITIDEGSLQSKQLLEVEDKGNELENDCSQLFEIDGGEHENGREETTGVDSHSEESQRTKDYAQPVEGVEFETYDDAYNYYNSYAREIGFAIRVKSSWTKRNSKEKRGAVLCCSCEGFKTNKEANSRRKETRTGCLAMIRLRVVESNRWRVDEVKLEHNHSFDPERAQNSKSHRRMDSGAKRKVEPTLDVEVRTIKLYRMPTVDASGYGSSNSNEGGTSNNVKFSRRLKLKKGDAELISNYFCHHKLVSPNFFYVMDLNDDGQLRNIFWIDSSSRAAYSYFGDVVAFDSTYLSNNYEIPLVAFVGVNHHGQSVLLGCGLLADETFETYTWLFRAWLSCMSSRPPETIVTNRCKTIECAIVEVFPRAHHRICLLQVLQIIRGCLVQFQEDEAFQMALTRVIYDPKTLDEFERDWDSLTQHYAIRNHAKLRSLHEDRELWAPVYSKDTFFAGISNYEKGESMIPFFKGHVHQQTSLKEFFEIYELVQQKKQKTEALDDLESQNSSPQLKTRCHYELQLSKLYTNAIFNKFQDEVVMMSSCFSVSQIQTNESIVTYMVKEHQGEEPVRNDRHFEVMYDKALTEVRCICNCFNFKGYLCRHALYILNYNGVGEIPCHYILSRWRKDFKRLYVPHLSSDNVDITNPVQCFDHLYKRGMQVVEEGTISQNHYLVSWQTFIESLNKVAAVKAIKPCSKYVFGGPTYMNYDKEK