AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02021

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02021.t1 MTR_6g051425 29.042 334 199 7 39 344 1 324 5.09e-34 133
MS.gene02021.t1 MTR_4g067290 35.644 101 62 1 65 162 56 156 2.47e-14 73.6
MS.gene02021.t1 MTR_4g067290 35.644 101 62 1 65 162 56 156 2.58e-14 73.6
MS.gene02021.t1 MTR_4g067290 35.644 101 62 1 65 162 76 176 3.15e-14 73.6
MS.gene02021.t1 MTR_4g067290 35.644 101 62 1 65 162 74 174 3.20e-14 73.6
MS.gene02021.t1 MTR_4g067290 35.644 101 62 1 65 162 76 176 3.28e-14 73.6
MS.gene02021.t1 MTR_4g067290 35.644 101 62 1 65 162 116 216 6.82e-14 73.6
MS.gene02021.t1 MTR_2g098880 35.849 106 58 2 65 162 61 164 1.18e-12 68.9
MS.gene02021.t1 MTR_2g098920 34.653 101 63 1 65 162 80 180 1.38e-12 68.9
MS.gene02021.t1 MTR_2g098880 35.849 106 58 2 65 162 73 176 1.41e-12 68.9
MS.gene02021.t1 MTR_7g446520 25.316 237 159 5 69 300 92 315 1.11e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02021.t1 AT1G52520 63.275 629 218 7 59 682 79 699 0.0 849
MS.gene02021.t1 AT1G80010 47.319 634 313 9 63 681 93 720 0.0 579
MS.gene02021.t1 AT4G15090 33.968 630 397 8 65 685 51 670 1.16e-121 382
MS.gene02021.t1 AT4G15090 33.968 630 397 8 65 685 51 670 1.16e-121 382
MS.gene02021.t1 AT4G15090 33.968 630 397 8 65 685 51 670 1.16e-121 382
MS.gene02021.t1 AT4G15090 33.968 630 397 8 65 685 51 670 1.16e-121 382
MS.gene02021.t1 AT4G15090 33.968 630 397 8 65 685 51 670 1.16e-121 382
MS.gene02021.t1 AT3G22170 33.480 681 386 12 21 675 29 668 2.69e-117 371
MS.gene02021.t1 AT3G22170 33.480 681 386 12 21 675 29 668 2.69e-117 371
MS.gene02021.t1 AT1G76320 34.817 629 358 13 69 677 1 597 2.78e-108 343
MS.gene02021.t1 AT1G76320 34.817 629 358 13 69 677 1 597 9.08e-108 343
MS.gene02021.t1 AT1G76320 34.817 629 358 13 69 677 1 597 9.08e-108 343
MS.gene02021.t1 AT1G76320 34.817 629 358 13 69 677 1 597 1.33e-107 343
MS.gene02021.t1 AT4G19990 34.106 604 333 9 39 622 1 559 9.28e-102 328
MS.gene02021.t1 AT4G19990 34.106 604 333 9 39 622 1 559 9.28e-102 328
MS.gene02021.t1 AT4G19990 34.106 604 333 9 39 622 1 559 2.31e-101 327
MS.gene02021.t1 AT4G19990 34.106 604 333 9 39 622 1 559 2.66e-101 327
MS.gene02021.t1 AT4G19990 34.106 604 333 9 39 622 1 559 2.66e-101 327
MS.gene02021.t1 AT4G19990 34.276 601 330 9 42 622 18 573 3.20e-101 327
MS.gene02021.t1 AT4G38180 33.391 578 361 11 65 628 73 640 1.99e-99 323
MS.gene02021.t1 AT4G19990 35.478 513 288 7 115 622 28 502 1.62e-92 302
MS.gene02021.t1 AT2G27110 29.467 638 410 12 49 682 38 639 8.70e-90 298
MS.gene02021.t1 AT2G27110 29.467 638 410 12 49 682 38 639 8.70e-90 298
MS.gene02021.t1 AT3G06250 29.874 636 387 13 51 678 176 760 8.86e-88 291
MS.gene02021.t1 AT3G06250 32.692 104 61 1 65 168 28 122 4.12e-11 67.0
MS.gene02021.t1 AT3G06250 29.874 636 387 13 51 678 176 760 8.86e-88 291
MS.gene02021.t1 AT3G06250 32.692 104 61 1 65 168 28 122 4.12e-11 67.0
MS.gene02021.t1 AT3G06250 29.874 636 387 13 51 678 176 760 8.86e-88 291
MS.gene02021.t1 AT3G06250 32.692 104 61 1 65 168 28 122 4.12e-11 67.0
MS.gene02021.t1 AT5G18960 30.695 619 379 15 65 678 211 784 7.99e-86 286
MS.gene02021.t1 AT5G18960 30.695 619 379 15 65 678 211 784 7.99e-86 286
MS.gene02021.t1 AT2G27110 30.962 478 318 5 207 682 27 494 1.05e-80 271
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 180 550 9.02e-78 264
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 139 509 1.14e-77 264
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 139 509 1.27e-77 264
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.65e-77 264
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.65e-77 264
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.69e-77 264
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.69e-77 264
MS.gene02021.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.69e-77 264
MS.gene02021.t1 AT4G38170 31.180 449 291 8 235 678 21 456 5.88e-72 243
MS.gene02021.t1 AT1G10240 26.258 636 427 15 65 680 48 661 1.45e-54 199
MS.gene02021.t1 AT1G10240 26.258 636 427 15 65 680 48 661 1.45e-54 199
MS.gene02021.t1 AT5G28530 25.966 647 426 18 64 681 54 676 4.98e-47 177
MS.gene02021.t1 AT5G28530 26.121 647 421 18 64 681 54 672 1.97e-46 176
MS.gene02021.t1 AT5G28530 26.121 647 421 18 64 681 54 672 1.97e-46 176
MS.gene02021.t1 AT5G28530 28.052 385 247 11 304 681 252 613 2.91e-30 127
MS.gene02021.t1 AT5G28530 28.312 385 242 11 304 681 252 609 8.02e-30 125
MS.gene02021.t1 AT3G59470 37.624 101 60 1 65 162 70 170 5.29e-14 72.8
MS.gene02021.t1 AT3G59470 37.624 101 60 1 65 162 70 170 5.29e-14 72.8
MS.gene02021.t1 AT3G59470 37.624 101 60 1 65 162 82 182 5.62e-14 73.2

Find 119 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTTGAGGAATGTATTA+TGG 0.176053 2.2:-59024701 MS.gene02021:CDS
CATGGTCAATCTGTGTTATT+AGG 0.198497 2.2:-59024561 MS.gene02021:CDS
CGTCGACGGTGTCAAGCTTT+TGG 0.198914 2.2:+59025720 None:intergenic
CCCAGATCTCATCATTGTTT+TGG 0.201438 2.2:-59024396 MS.gene02021:CDS
CAATGGTCTAATCAGTTATT+TGG 0.205211 2.2:-59023556 MS.gene02021:CDS
GAAGAAATGTTCTCTTGTTT+TGG 0.291820 2.2:-59023889 MS.gene02021:CDS
TCCATCAAATAAAAGAAGTT+TGG 0.301368 2.2:+59024738 None:intergenic
AACTTGTCCTTTATGTATAC+TGG 0.304465 2.2:+59024168 None:intergenic
CTGCAGTTCAAAAGAGCATT+TGG 0.320519 2.2:+59023963 None:intergenic
ACGACATAGGAAATTATAAA+TGG 0.326434 2.2:+59024776 None:intergenic
TTAGGTTGTGGGTTGCTTGC+AGG 0.332899 2.2:-59024543 MS.gene02021:CDS
TACATAAAGGACAAGTTTCT+TGG 0.335040 2.2:-59024162 MS.gene02021:CDS
AATGAACTCGGTCTTCATAT+AGG 0.339160 2.2:+59024196 None:intergenic
ATGAACTCGGTCTTCATATA+GGG 0.353735 2.2:+59024197 None:intergenic
TGGAAGTGCCTTACAAGTTA+TGG 0.355565 2.2:-59023536 MS.gene02021:CDS
TGGCTCCATCATAATCTTCT+TGG 0.357834 2.2:-59023845 MS.gene02021:CDS
CACACAAAATCCCACCTCTT+TGG 0.362616 2.2:+59025223 None:intergenic
CATGGCAGGACAACCAGTTC+TGG 0.364832 2.2:+59025085 None:intergenic
CTCGAACATAACCACGTCTT+AGG 0.365078 2.2:-59025011 MS.gene02021:CDS
ACATTTGAGGAATGTATTAT+GGG 0.365589 2.2:-59024700 MS.gene02021:CDS
GCCAAAACAATGATGAGATC+TGG 0.365658 2.2:+59024395 None:intergenic
TTCTCCTCAAACTATTATTA+CGG 0.379468 2.2:-59024460 MS.gene02021:CDS
GAATTTCCCGCACCTGCTCT+TGG 0.386074 2.2:-59025296 MS.gene02021:CDS
GTTCAGACATTCACCTTTGA+AGG 0.390130 2.2:-59024082 MS.gene02021:CDS
CCATTACGATGCAGCGCGTC+AGG 0.390140 2.2:-59023491 MS.gene02021:CDS
CAATCTGTGTTATTAGGTTG+TGG 0.392493 2.2:-59024555 MS.gene02021:CDS
GGGAAACAGACAGGAGATAA+AGG 0.399801 2.2:-59023800 MS.gene02021:CDS
AAAGGACTAATGCTCTTACT+AGG 0.401008 2.2:+59024120 None:intergenic
ATTGAAAGTGATTGAATTTG+AGG 0.404070 2.2:-59024277 MS.gene02021:CDS
TTGATGGATTTCAATGATGA+AGG 0.429652 2.2:-59024723 MS.gene02021:CDS
ATTACCTGGCATTCTCCATC+GGG 0.434341 2.2:+59025359 None:intergenic
ACATGGGTTACTTGTATGTC+AGG 0.436090 2.2:-59024486 MS.gene02021:CDS
AAAATAACGACATGCAGCTT+TGG 0.449608 2.2:+59024665 None:intergenic
AATCTGTGTTATTAGGTTGT+GGG 0.451302 2.2:-59024554 MS.gene02021:CDS
TATATGAAGACCGAGTTCAT+TGG 0.452679 2.2:-59024194 MS.gene02021:CDS
GATTGAATTTGAGGCGGCAT+GGG 0.459082 2.2:-59024268 MS.gene02021:CDS
AGTTGTGATCAGGAATATAA+AGG 0.460169 2.2:+59023608 None:intergenic
CATGATAAGGATTAAGTTAG+TGG 0.463158 2.2:-59025066 MS.gene02021:CDS
TTGAGAAAAGAAACCAGAAC+TGG 0.466660 2.2:-59025098 MS.gene02021:CDS
ATATGAAGACCGAGTTCATT+GGG 0.473754 2.2:-59024193 MS.gene02021:CDS
AGCTCTGTCATCTGTTGCAT+TGG 0.476249 2.2:+59024848 None:intergenic
TTCTGCAATTGCACTTTGCA+AGG 0.480040 2.2:+59024425 None:intergenic
TCCCGCACCTGCTCTTGGAA+TGG 0.481909 2.2:-59025291 MS.gene02021:CDS
CTGGTTGTCCTGCCATGATA+AGG 0.483669 2.2:-59025079 MS.gene02021:CDS
CCAAAACAATGATGAGATCT+GGG 0.492319 2.2:+59024396 None:intergenic
GTGCTTTGTTGTAGCAGTCA+AGG 0.493576 2.2:-59025149 MS.gene02021:CDS
TCCCTCCTCCATAACTTGTA+AGG 0.494681 2.2:+59023528 None:intergenic
AAACGACCGAATCGTATTCA+TGG 0.494788 2.2:-59024518 MS.gene02021:CDS
AATTACCTGGCATTCTCCAT+CGG 0.497269 2.2:+59025358 None:intergenic
TTTGACCAAGAAGATTATGA+TGG 0.497729 2.2:+59023840 None:intergenic
GAACTACCAGAGTTGTGATC+AGG 0.499730 2.2:+59023598 None:intergenic
TGTTCTTGTTCTTCTTCTTG+AGG 0.500286 2.2:+59025686 None:intergenic
ATGTATTATGGGTTGATGCC+AGG 0.501404 2.2:-59024689 MS.gene02021:CDS
TTTGATTGAGGGAAACAGAC+AGG 0.503735 2.2:-59023809 MS.gene02021:CDS
CATGAAGTTCCAACTTGAAG+TGG 0.504481 2.2:-59023911 MS.gene02021:CDS
GGACAAGTTTCTTGGTGGAA+TGG 0.506245 2.2:-59024154 MS.gene02021:CDS
TTGGGCACCAGTATACATAA+AGG 0.506630 2.2:-59024175 MS.gene02021:CDS
AATAAGTTGAACCTAAGAAA+AGG 0.515686 2.2:-59024810 MS.gene02021:CDS
TGATTGAATTTGAGGCGGCA+TGG 0.516224 2.2:-59024269 MS.gene02021:CDS
ACATTTCTTCCACTTCAAGT+TGG 0.516480 2.2:+59023902 None:intergenic
AACTTAATCCTTATCATGGC+AGG 0.517948 2.2:+59025071 None:intergenic
AAGGACTAATGCTCTTACTA+GGG 0.518402 2.2:+59024121 None:intergenic
TTCATGGTTGTTTAGAACAT+GGG 0.523651 2.2:-59024502 MS.gene02021:CDS
TTCCATTCCAAGAGCAGGTG+CGG 0.528014 2.2:+59025289 None:intergenic
ATGATATTGAACGTATTCAA+TGG 0.532271 2.2:-59023573 MS.gene02021:CDS
ATTCATGGTTGTTTAGAACA+TGG 0.534938 2.2:-59024503 MS.gene02021:CDS
ATAAAGGACAAGTTTCTTGG+TGG 0.535782 2.2:-59024159 MS.gene02021:CDS
CACTAACTTAATCCTTATCA+TGG 0.537066 2.2:+59025067 None:intergenic
TCCATTCCAAGAGCAGGTGC+GGG 0.537722 2.2:+59025290 None:intergenic
GTGATAGATACAGAAGGTAA+TGG 0.538353 2.2:-59024882 MS.gene02021:CDS
TTGCCTACCTCTGATGCGGA+GGG 0.539878 2.2:-59024933 MS.gene02021:CDS
TATATTCCTGATCACAACTC+TGG 0.540777 2.2:-59023604 MS.gene02021:CDS
TCAAATTCCATTCCAAGAGC+AGG 0.541895 2.2:+59025284 None:intergenic
TGCCTTACAAGTTATGGAGG+AGG 0.551483 2.2:-59023530 MS.gene02021:CDS
TGTCAAGATGATATTTCTGA+AGG 0.552931 2.2:-59025326 MS.gene02021:CDS
AAACAACCATGAATACGATT+CGG 0.553116 2.2:+59024512 None:intergenic
ACGACATGCAGCTTTGGACC+TGG 0.553268 2.2:+59024671 None:intergenic
ATTACCCGATGGAGAATGCC+AGG 0.553334 2.2:-59025363 MS.gene02021:CDS
CCTGACGCGCTGCATCGTAA+TGG 0.562464 2.2:+59023491 None:intergenic
TCAATGATGAAGGACATTTG+AGG 0.564984 2.2:-59024713 MS.gene02021:CDS
ATTTGCTATGCCAAAGAGGT+GGG 0.566055 2.2:-59025233 MS.gene02021:CDS
ATCTCTTTATAAGAAGTACA+AGG 0.566116 2.2:-59024037 MS.gene02021:CDS
ACCACTCAACTACGTGCAGA+TGG 0.567203 2.2:-59023865 MS.gene02021:CDS
AGTTAGTGGAGTCTCAGAGA+TGG 0.573080 2.2:-59025052 MS.gene02021:CDS
TAGTTAGCTGCATACGACAT+AGG 0.573239 2.2:+59024763 None:intergenic
AGGTTGAGACTCGCCGTCGA+CGG 0.574630 2.2:+59025706 None:intergenic
TATTTGCTATGCCAAAGAGG+TGG 0.575475 2.2:-59025234 MS.gene02021:CDS
ATTCCAACAAAAGTCACAAG+AGG 0.580554 2.2:+59024591 None:intergenic
GTTGCCTACCTCTGATGCGG+AGG 0.580823 2.2:-59024934 MS.gene02021:CDS
CTATCCGTAATAATAGTTTG+AGG 0.582240 2.2:+59024456 None:intergenic
GACAATCTAATTCAATTACC+TGG 0.587039 2.2:+59025345 None:intergenic
TTGTCAAGAAAATCCTTCAA+AGG 0.587595 2.2:+59024069 None:intergenic
TAGTGGAGTCTCAGAGATGG+AGG 0.590281 2.2:-59025049 MS.gene02021:CDS
GCCTTACAAGTTATGGAGGA+GGG 0.592013 2.2:-59023529 MS.gene02021:CDS
CACCGTGATAGAATGTACTT+AGG 0.598150 2.2:+59023649 None:intergenic
CGTGATAGAATGTACTTAGG+AGG 0.599389 2.2:+59023652 None:intergenic
ATACTGGTGCCCAATGAACT+CGG 0.607374 2.2:+59024184 None:intergenic
ATTGAATTTGAGGCGGCATG+GGG 0.607914 2.2:-59024267 MS.gene02021:CDS
TTATATTTGCTATGCCAAAG+AGG 0.610702 2.2:-59025237 MS.gene02021:CDS
CTCGTTGCCTACCTCTGATG+CGG 0.623507 2.2:-59024937 MS.gene02021:CDS
TGCCTACCTCTGATGCGGAG+GGG 0.624908 2.2:-59024932 MS.gene02021:CDS
GCCATCTGCACGTAGTTGAG+TGG 0.627400 2.2:+59023864 None:intergenic
CTCCTAAGTACATTCTATCA+CGG 0.629356 2.2:-59023651 MS.gene02021:CDS
TTCATGATGTAAAACTTGAG+AGG 0.630957 2.2:-59023444 MS.gene02021:CDS
CAAAAGCTTGACACCGTCGA+CGG 0.630980 2.2:-59025719 MS.gene02021:CDS
AAGTGCCTTACAAGTTATGG+AGG 0.637921 2.2:-59023533 MS.gene02021:CDS
TTCCCCTCCGCATCAGAGGT+AGG 0.638609 2.2:+59024930 None:intergenic
GTATCTTAGCACCTAAGACG+TGG 0.639364 2.2:+59025000 None:intergenic
TTTGTTGGAATAAATCACCA+TGG 0.640231 2.2:-59024579 MS.gene02021:CDS
AGAAGAACAAGAACACCCAA+AGG 0.641728 2.2:-59025678 MS.gene02021:intron
CTCAAACTATTATTACGGAT+AGG 0.645642 2.2:-59024455 MS.gene02021:CDS
GAAAGTGATTGAATTTGAGG+CGG 0.650309 2.2:-59024274 MS.gene02021:CDS
TTGAAGTTAGGGATAACGAG+TGG 0.652213 2.2:-59024227 MS.gene02021:CDS
CTAATAACACAGATTGACCA+TGG 0.669427 2.2:+59024562 None:intergenic
GCACTTGTGATAGATACAGA+AGG 0.672329 2.2:-59024888 MS.gene02021:CDS
TAAAGCAAGAACAAATACAA+CGG 0.685448 2.2:+59023740 None:intergenic
TCAGAGATGGAGGATATGTG+AGG 0.701673 2.2:-59025039 MS.gene02021:CDS
TGTTGTGATCAATTACCCGA+TGG 0.706322 2.2:-59025374 MS.gene02021:CDS
CTAAGTACATTCTATCACGG+TGG 0.712046 2.2:-59023648 MS.gene02021:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATAAATTAGTTAAAAAGA+TGG - chr2.2:59024202-59024221 MS.gene02021:CDS 15.0%
!! ATGAAAAATTAATTACCTTT+GGG + chr2.2:59023521-59023540 None:intergenic 15.0%
!! TATGAAAAATTAATTACCTT+TGG + chr2.2:59023522-59023541 None:intergenic 15.0%
!! AACATGTATTTATCGAAAAA+AGG + chr2.2:59025082-59025101 None:intergenic 20.0%
!! TGTATCTAACTTATTTCAAA+TGG + chr2.2:59023615-59023634 None:intergenic 20.0%
!!! ATTTTTATGGATATCTATGT+CGG - chr2.2:59025464-59025483 MS.gene02021:intron 20.0%
!!! TGTTCTTGCTTTAATTTTTA+TGG - chr2.2:59025451-59025470 MS.gene02021:intron 20.0%
!!! TTTTGAAGAATCACTTAATA+AGG - chr2.2:59025714-59025733 MS.gene02021:CDS 20.0%
! ACATTTGAGGAATGTATTAT+GGG - chr2.2:59024481-59024500 MS.gene02021:CDS 25.0%
! ACGACATAGGAAATTATAAA+TGG + chr2.2:59024408-59024427 None:intergenic 25.0%
! ATGATATTGAACGTATTCAA+TGG - chr2.2:59025608-59025627 MS.gene02021:intron 25.0%
! ATTGAAAGTGATTGAATTTG+AGG - chr2.2:59024904-59024923 MS.gene02021:CDS 25.0%
! CGAAATAGCAAAGAAAAATA+TGG - chr2.2:59024005-59024024 MS.gene02021:CDS 25.0%
! TAAAGCAAGAACAAATACAA+CGG + chr2.2:59025444-59025463 None:intergenic 25.0%
! TCCATCAAATAAAAGAAGTT+TGG + chr2.2:59024446-59024465 None:intergenic 25.0%
! TTCTCCTCAAACTATTATTA+CGG - chr2.2:59024721-59024740 MS.gene02021:CDS 25.0%
!! AATAAGTTGAACCTAAGAAA+AGG - chr2.2:59024371-59024390 MS.gene02021:CDS 25.0%
!! ATCTCTTTATAAGAAGTACA+AGG - chr2.2:59025144-59025163 MS.gene02021:CDS 25.0%
!!! AAAAAAGTACCAGAAAAACT+TGG - chr2.2:59024824-59024843 MS.gene02021:CDS 25.0%
!!! GATTCAACATTTTGAAGTTA+GGG - chr2.2:59024943-59024962 MS.gene02021:CDS 25.0%
!!! TCCAAACTTCTTTTATTTGA+TGG - chr2.2:59024442-59024461 MS.gene02021:CDS 25.0%
!!! TGATTCAACATTTTGAAGTT+AGG - chr2.2:59024942-59024961 MS.gene02021:CDS 25.0%
!!! TGTGTGTACTCAATTTTAAT+GGG - chr2.2:59025494-59025513 MS.gene02021:intron 25.0%
AAACAACCATGAATACGATT+CGG + chr2.2:59024672-59024691 None:intergenic 30.0%
AACTTGTCCTTTATGTATAC+TGG + chr2.2:59025016-59025035 None:intergenic 30.0%
AGTTGTGATCAGGAATATAA+AGG + chr2.2:59025576-59025595 None:intergenic 30.0%
CAATGGTCTAATCAGTTATT+TGG - chr2.2:59025625-59025644 MS.gene02021:intron 30.0%
CACTAACTTAATCCTTATCA+TGG + chr2.2:59024117-59024136 None:intergenic 30.0%
CATGATAAGGATTAAGTTAG+TGG - chr2.2:59024115-59024134 MS.gene02021:CDS 30.0%
CTATCCGTAATAATAGTTTG+AGG + chr2.2:59024728-59024747 None:intergenic 30.0%
CTCAAACTATTATTACGGAT+AGG - chr2.2:59024726-59024745 MS.gene02021:CDS 30.0%
GACAATCTAATTCAATTACC+TGG + chr2.2:59023839-59023858 None:intergenic 30.0%
GACATTTGAGGAATGTATTA+TGG - chr2.2:59024480-59024499 MS.gene02021:CDS 30.0%
TACATAAAGGACAAGTTTCT+TGG - chr2.2:59025019-59025038 MS.gene02021:CDS 30.0%
TTAGTTAAAAAGATGGCAAG+TGG - chr2.2:59024209-59024228 MS.gene02021:CDS 30.0%
TTATGAATTACCGTAGATCT+TGG + chr2.2:59023565-59023584 None:intergenic 30.0%
TTCATGATGTAAAACTTGAG+AGG - chr2.2:59025737-59025756 MS.gene02021:CDS 30.0%
TTGTCAAGAAAATCCTTCAA+AGG + chr2.2:59025115-59025134 None:intergenic 30.0%
TTTGACCAAGAAGATTATGA+TGG + chr2.2:59025344-59025363 None:intergenic 30.0%
TTTGTTGGAATAAATCACCA+TGG - chr2.2:59024602-59024621 MS.gene02021:CDS 30.0%
! AATCTGTGTTATTAGGTTGT+GGG - chr2.2:59024627-59024646 MS.gene02021:CDS 30.0%
! ATTCATGGTTGTTTAGAACA+TGG - chr2.2:59024678-59024697 MS.gene02021:CDS 30.0%
! CTGTGTGTACTCAATTTTAA+TGG - chr2.2:59025493-59025512 MS.gene02021:intron 30.0%
! GTCAATTTAGATTTTGCGTT+AGG - chr2.2:59023715-59023734 MS.gene02021:CDS 30.0%
! TGTCAAGATGATATTTCTGA+AGG - chr2.2:59023855-59023874 MS.gene02021:CDS 30.0%
! TTCATGGTTGTTTAGAACAT+GGG - chr2.2:59024679-59024698 MS.gene02021:CDS 30.0%
!! ATTTTGTTTGACGATATGCT+AGG - chr2.2:59023767-59023786 MS.gene02021:CDS 30.0%
!! GAAGAAATGTTCTCTTGTTT+TGG - chr2.2:59025292-59025311 MS.gene02021:CDS 30.0%
!! GTGTGTACTCAATTTTAATG+GGG - chr2.2:59025495-59025514 MS.gene02021:intron 30.0%
!! TTATATTTGCTATGCCAAAG+AGG - chr2.2:59023944-59023963 MS.gene02021:CDS 30.0%
!! TTGATGGATTTCAATGATGA+AGG - chr2.2:59024458-59024477 MS.gene02021:CDS 30.0%
AAAATAACGACATGCAGCTT+TGG + chr2.2:59024519-59024538 None:intergenic 35.0%
AAAGGACTAATGCTCTTACT+AGG + chr2.2:59025064-59025083 None:intergenic 35.0%
AACTTAATCCTTATCATGGC+AGG + chr2.2:59024113-59024132 None:intergenic 35.0%
AAGGACTAATGCTCTTACTA+GGG + chr2.2:59025063-59025082 None:intergenic 35.0%
AAGTACCAGAAAAACTTGGA+GGG - chr2.2:59024828-59024847 MS.gene02021:CDS 35.0%
AATGAACTCGGTCTTCATAT+AGG + chr2.2:59024988-59025007 None:intergenic 35.0%
ACATTTCTTCCACTTCAAGT+TGG + chr2.2:59025282-59025301 None:intergenic 35.0%
ATAAAGGACAAGTTTCTTGG+TGG - chr2.2:59025022-59025041 MS.gene02021:CDS 35.0%
ATATGAAGACCGAGTTCATT+GGG - chr2.2:59024988-59025007 MS.gene02021:CDS 35.0%
ATGAACTCGGTCTTCATATA+GGG + chr2.2:59024987-59025006 None:intergenic 35.0%
ATTCCAACAAAAGTCACAAG+AGG + chr2.2:59024593-59024612 None:intergenic 35.0%
CCAAAACAATGATGAGATCT+GGG + chr2.2:59024788-59024807 None:intergenic 35.0%
CTAATAACACAGATTGACCA+TGG + chr2.2:59024622-59024641 None:intergenic 35.0%
CTCCTAAGTACATTCTATCA+CGG - chr2.2:59025530-59025549 MS.gene02021:intron 35.0%
GAAAGTGATTGAATTTGAGG+CGG - chr2.2:59024907-59024926 MS.gene02021:CDS 35.0%
GTGATAGATACAGAAGGTAA+TGG - chr2.2:59024299-59024318 MS.gene02021:CDS 35.0%
TATATGAAGACCGAGTTCAT+TGG - chr2.2:59024987-59025006 MS.gene02021:CDS 35.0%
TATATTCCTGATCACAACTC+TGG - chr2.2:59025577-59025596 MS.gene02021:intron 35.0%
TCAATGATGAAGGACATTTG+AGG - chr2.2:59024468-59024487 MS.gene02021:CDS 35.0%
TGTTCTTGTTCTTCTTCTTG+AGG + chr2.2:59023498-59023517 None:intergenic 35.0%
TTGAGAAAAGAAACCAGAAC+TGG - chr2.2:59024083-59024102 MS.gene02021:CDS 35.0%
! AAAGTACCAGAAAAACTTGG+AGG - chr2.2:59024827-59024846 MS.gene02021:CDS 35.0%
! ATTCCTCTTGTGACTTTTGT+TGG - chr2.2:59024587-59024606 MS.gene02021:CDS 35.0%
! CAATCTGTGTTATTAGGTTG+TGG - chr2.2:59024626-59024645 MS.gene02021:CDS 35.0%
! CATGGTCAATCTGTGTTATT+AGG - chr2.2:59024620-59024639 MS.gene02021:CDS 35.0%
!! TGTACTCAATTTTAATGGGG+TGG - chr2.2:59025498-59025517 MS.gene02021:intron 35.0%
!!! AAAGAGCGTGTTTTGATTGA+GGG - chr2.2:59025361-59025380 MS.gene02021:CDS 35.0%
AAACGACCGAATCGTATTCA+TGG - chr2.2:59024663-59024682 MS.gene02021:CDS 40.0%
AAGTGCCTTACAAGTTATGG+AGG - chr2.2:59025648-59025667 MS.gene02021:intron 40.0%
AATTACCTGGCATTCTCCAT+CGG + chr2.2:59023826-59023845 None:intergenic 40.0%
AGAAGAACAAGAACACCCAA+AGG - chr2.2:59023503-59023522 MS.gene02021:CDS 40.0%
CACCGTGATAGAATGTACTT+AGG + chr2.2:59025535-59025554 None:intergenic 40.0%
CATGAAGTTCCAACTTGAAG+TGG - chr2.2:59025270-59025289 MS.gene02021:CDS 40.0%
CGTGATAGAATGTACTTAGG+AGG + chr2.2:59025532-59025551 None:intergenic 40.0%
CTAAGTACATTCTATCACGG+TGG - chr2.2:59025533-59025552 MS.gene02021:intron 40.0%
GACATTAAATTTCGCTACCC+AGG + chr2.2:59023698-59023717 None:intergenic 40.0%
GCACTTGTGATAGATACAGA+AGG - chr2.2:59024293-59024312 MS.gene02021:CDS 40.0%
GCCAAAACAATGATGAGATC+TGG + chr2.2:59024789-59024808 None:intergenic 40.0%
GTTCAGACATTCACCTTTGA+AGG - chr2.2:59025099-59025118 MS.gene02021:CDS 40.0%
TAGGCACGCTGATATTGTTT+AGG - chr2.2:59023734-59023753 MS.gene02021:CDS 40.0%
TAGTTAGCTGCATACGACAT+AGG + chr2.2:59024421-59024440 None:intergenic 40.0%
TCAAATTCCATTCCAAGAGC+AGG + chr2.2:59023900-59023919 None:intergenic 40.0%
TGGCTCCATCATAATCTTCT+TGG - chr2.2:59025336-59025355 MS.gene02021:CDS 40.0%
TGTTGTGATCAATTACCCGA+TGG - chr2.2:59023807-59023826 MS.gene02021:CDS 40.0%
TTACCGTAGATCTTGGAAAG+AGG + chr2.2:59023558-59023577 None:intergenic 40.0%
TTCTGCAATTGCACTTTGCA+AGG + chr2.2:59024759-59024778 None:intergenic 40.0%
TTGAAGTTAGGGATAACGAG+TGG - chr2.2:59024954-59024973 MS.gene02021:CDS 40.0%
TTGCCTCTTTCCAAGATCTA+CGG - chr2.2:59023552-59023571 MS.gene02021:CDS 40.0%
TTGGGCACCAGTATACATAA+AGG - chr2.2:59025006-59025025 MS.gene02021:CDS 40.0%
TTGTTTGACGATATGCTAGG+TGG - chr2.2:59023770-59023789 MS.gene02021:CDS 40.0%
TTTGATTGAGGGAAACAGAC+AGG - chr2.2:59025372-59025391 MS.gene02021:CDS 40.0%
! ACATGGGTTACTTGTATGTC+AGG - chr2.2:59024695-59024714 MS.gene02021:CDS 40.0%
! CCCAGATCTCATCATTGTTT+TGG - chr2.2:59024785-59024804 MS.gene02021:CDS 40.0%
! CTTGTGAGTCACCTTTTCTT+AGG + chr2.2:59024385-59024404 None:intergenic 40.0%
!! ATGTATTATGGGTTGATGCC+AGG - chr2.2:59024492-59024511 MS.gene02021:CDS 40.0%
!! ATTTGCTATGCCAAAGAGGT+GGG - chr2.2:59023948-59023967 MS.gene02021:CDS 40.0%
!! CTGCAGTTCAAAAGAGCATT+TGG + chr2.2:59025221-59025240 None:intergenic 40.0%
!! TATTTGCTATGCCAAAGAGG+TGG - chr2.2:59023947-59023966 MS.gene02021:CDS 40.0%
!! TGGAAGTGCCTTACAAGTTA+TGG - chr2.2:59025645-59025664 MS.gene02021:intron 40.0%
!!! AGAGCATTTGGTTTTCAGCA+AGG + chr2.2:59025209-59025228 None:intergenic 40.0%
!!! AGCATTTGGTTTTCAGCAAG+GGG + chr2.2:59025207-59025226 None:intergenic 40.0%
!!! CAAAGAGCGTGTTTTGATTG+AGG - chr2.2:59025360-59025379 MS.gene02021:CDS 40.0%
!!! GAGCATTTGGTTTTCAGCAA+GGG + chr2.2:59025208-59025227 None:intergenic 40.0%
ACCTCTTGTGTAGGAAACCT+GGG - chr2.2:59023678-59023697 MS.gene02021:CDS 45.0%
AGCTCTGTCATCTGTTGCAT+TGG + chr2.2:59024336-59024355 None:intergenic 45.0%
AGTTAGTGGAGTCTCAGAGA+TGG - chr2.2:59024129-59024148 MS.gene02021:CDS 45.0%
ATACTGGTGCCCAATGAACT+CGG + chr2.2:59025000-59025019 None:intergenic 45.0%
ATGCTGCATACCTCTTGTGT+AGG - chr2.2:59023669-59023688 MS.gene02021:CDS 45.0%
ATTACCTGGCATTCTCCATC+GGG + chr2.2:59023825-59023844 None:intergenic 45.0%
CACACAAAATCCCACCTCTT+TGG + chr2.2:59023961-59023980 None:intergenic 45.0%
CTCGAACATAACCACGTCTT+AGG - chr2.2:59024170-59024189 MS.gene02021:CDS 45.0%
GAACTACCAGAGTTGTGATC+AGG + chr2.2:59025586-59025605 None:intergenic 45.0%
GCCTTACAAGTTATGGAGGA+GGG - chr2.2:59025652-59025671 MS.gene02021:intron 45.0%
GGACAAGTTTCTTGGTGGAA+TGG - chr2.2:59025027-59025046 MS.gene02021:CDS 45.0%
GGGAAACAGACAGGAGATAA+AGG - chr2.2:59025381-59025400 MS.gene02021:CDS 45.0%
GTATCTTAGCACCTAAGACG+TGG + chr2.2:59024184-59024203 None:intergenic 45.0%
TACCTCTTGTGTAGGAAACC+TGG - chr2.2:59023677-59023696 MS.gene02021:CDS 45.0%
TCAGAGATGGAGGATATGTG+AGG - chr2.2:59024142-59024161 MS.gene02021:CDS 45.0%
TGCCTTACAAGTTATGGAGG+AGG - chr2.2:59025651-59025670 MS.gene02021:intron 45.0%
! AAGAGGTGGGATTTTGTGTG+AGG - chr2.2:59023961-59023980 MS.gene02021:CDS 45.0%
! ATTGAATTTGAGGCGGCATG+GGG - chr2.2:59024914-59024933 MS.gene02021:CDS 45.0%
! GATTGAATTTGAGGCGGCAT+GGG - chr2.2:59024913-59024932 MS.gene02021:CDS 45.0%
! GTGCTTTGTTGTAGCAGTCA+AGG - chr2.2:59024032-59024051 MS.gene02021:CDS 45.0%
! TCCCTCCTCCATAACTTGTA+AGG + chr2.2:59025656-59025675 None:intergenic 45.0%
! TGATTGAATTTGAGGCGGCA+TGG - chr2.2:59024912-59024931 MS.gene02021:CDS 45.0%
ACCACTCAACTACGTGCAGA+TGG - chr2.2:59025316-59025335 MS.gene02021:CDS 50.0%
ACCCAGGTTTCCTACACAAG+AGG + chr2.2:59023682-59023701 None:intergenic 50.0%
ATTACCCGATGGAGAATGCC+AGG - chr2.2:59023818-59023837 MS.gene02021:CDS 50.0%
CAAAAGCTTGACACCGTCGA+CGG - chr2.2:59023462-59023481 MS.gene02021:CDS 50.0%
CTGGTTGTCCTGCCATGATA+AGG - chr2.2:59024102-59024121 MS.gene02021:CDS 50.0%
TAGTGGAGTCTCAGAGATGG+AGG - chr2.2:59024132-59024151 MS.gene02021:CDS 50.0%
TTCCATTCCAAGAGCAGGTG+CGG + chr2.2:59023895-59023914 None:intergenic 50.0%
! CGCAACCCTCCAAGTTTTTC+TGG + chr2.2:59024836-59024855 None:intergenic 50.0%
! TTAGGTTGTGGGTTGCTTGC+AGG - chr2.2:59024638-59024657 MS.gene02021:CDS 50.0%
!! GTCGACGGTGTCAAGCTTTT+GGG + chr2.2:59023463-59023482 None:intergenic 50.0%
CATGGCAGGACAACCAGTTC+TGG + chr2.2:59024099-59024118 None:intergenic 55.0%
CTCGTTGCCTACCTCTGATG+CGG - chr2.2:59024244-59024263 MS.gene02021:CDS 55.0%
GAATTTCCCGCACCTGCTCT+TGG - chr2.2:59023885-59023904 MS.gene02021:CDS 55.0%
GCCATCTGCACGTAGTTGAG+TGG + chr2.2:59025320-59025339 None:intergenic 55.0%
TCCATTCCAAGAGCAGGTGC+GGG + chr2.2:59023894-59023913 None:intergenic 55.0%
TTGCCTACCTCTGATGCGGA+GGG - chr2.2:59024248-59024267 MS.gene02021:CDS 55.0%
! TGTTTTCCCCTCCGCATCAG+AGG + chr2.2:59024258-59024277 None:intergenic 55.0%
!! ACGACATGCAGCTTTGGACC+TGG + chr2.2:59024513-59024532 None:intergenic 55.0%
!! CGTCGACGGTGTCAAGCTTT+TGG + chr2.2:59023464-59023483 None:intergenic 55.0%
AGGTTGAGACTCGCCGTCGA+CGG + chr2.2:59023478-59023497 None:intergenic 60.0%
CCATTACGATGCAGCGCGTC+AGG - chr2.2:59025690-59025709 MS.gene02021:CDS 60.0%
CCTGACGCGCTGCATCGTAA+TGG + chr2.2:59025693-59025712 None:intergenic 60.0%
GTTGCCTACCTCTGATGCGG+AGG - chr2.2:59024247-59024266 MS.gene02021:CDS 60.0%
TCCCGCACCTGCTCTTGGAA+TGG - chr2.2:59023890-59023909 MS.gene02021:CDS 60.0%
TGCCTACCTCTGATGCGGAG+GGG - chr2.2:59024249-59024268 MS.gene02021:CDS 60.0%
TTCCCCTCCGCATCAGAGGT+AGG + chr2.2:59024254-59024273 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 59023441 59025762 59023441 ID=MS.gene02021
chr2.2 mRNA 59023441 59025762 59023441 ID=MS.gene02021.t1;Parent=MS.gene02021
chr2.2 exon 59025679 59025762 59025679 ID=MS.gene02021.t1.exon1;Parent=MS.gene02021.t1
chr2.2 CDS 59025679 59025762 59025679 ID=cds.MS.gene02021.t1;Parent=MS.gene02021.t1
chr2.2 exon 59023441 59025414 59023441 ID=MS.gene02021.t1.exon2;Parent=MS.gene02021.t1
chr2.2 CDS 59023441 59025414 59023441 ID=cds.MS.gene02021.t1;Parent=MS.gene02021.t1
Gene Sequence

>MS.gene02021

ATGATGTCAGAGTTGCAGACCCAAAAGCTTGACACCGTCGACGGCGAGTCTCAACCTCAAGAAGAAGAACAAGAACACCCAAAGGTAATTAATTTTTCATATATATGCTCTTTGCCTCTTTCCAAGATCTACGGTAATTCATAATGAAACAAAATCTCAATTTAATTGTAGCCATTTGAAATAAGTTAGATACATGATATATTTATGCAAGTAAGTGATTACATGTTTATGCTGCATACCTCTTGTGTAGGAAACCTGGGTAGCGAAATTTAATGTCAATTTAGATTTTGCGTTAGGCACGCTGATATTGTTTAGGTAGCTTATTGATTTTGTTTGACGATATGCTAGGTGGACGACATTTCTGTGTGTTGTGATCAATTACCCGATGGAGAATGCCAGGTAATTGAATTAGATTGTCAAGATGATATTTCTGAAGGAAGAAAAGAATTTCCCGCACCTGCTCTTGGAATGGAATTTGAGTCATATGATGATGCTTACAGTTATTATATTTGCTATGCCAAAGAGGTGGGATTTTGTGTGAGGATTAAGAATTCATGTTTCAAGCGAAATAGCAAAGAAAAATATGGTGCAGTGCTTTGTTGTAGCAGTCAAGGTTTCAATAGAACAAAAGATGTTGACATTTTGAGAAAAGAAACCAGAACTGGTTGTCCTGCCATGATAAGGATTAAGTTAGTGGAGTCTCAGAGATGGAGGATATGTGAGGTTACTCTCGAACATAACCACGTCTTAGGTGCTAAGATACATAAATTAGTTAAAAAGATGGCAAGTGGAACTAAAAAGAACTCGTTGCCTACCTCTGATGCGGAGGGGAAAACAATTAAGATATATCATGCACTTGTGATAGATACAGAAGGTAATGGCAATTTAAACTCCAATGCAACAGATGACAGAGCTTTCTCGAAATATTCTAATAAGTTGAACCTAAGAAAAGGTGACTCACAAGCCATTTATAATTTCCTATGTCGTATGCAGCTAACTAATCCAAACTTCTTTTATTTGATGGATTTCAATGATGAAGGACATTTGAGGAATGTATTATGGGTTGATGCCAGGTCCAAAGCTGCATGTCGTTATTTTAGCGATGTCATTTATTTTGACAACACTTATTTGTCAAATAAATATGAAATTCCTCTTGTGACTTTTGTTGGAATAAATCACCATGGTCAATCTGTGTTATTAGGTTGTGGGTTGCTTGCAGGTGAAACGACCGAATCGTATTCATGGTTGTTTAGAACATGGGTTACTTGTATGTCAGGATGTTCTCCTCAAACTATTATTACGGATAGGTGCAAGACCTTGCAAAGTGCAATTGCAGAAAATTTTCCCAGATCTCATCATTGTTTTGGCTTGTCATTAGTTATGAAAAAAGTACCAGAAAAACTTGGAGGGTTGCGCAATTACGATGCAATCAGAAAAGCACTGATTAAAGCAGTTTATGAGACATTGAAAGTGATTGAATTTGAGGCGGCATGGGGATTTATGATTCAACATTTTGAAGTTAGGGATAACGAGTGGCTGCTTTCCCTATATGAAGACCGAGTTCATTGGGCACCAGTATACATAAAGGACAAGTTTCTTGGTGGAATGGCTACTACACGCCCTAGTAAGAGCATTAGTCCTTTTTTCGATAAATACATGTTCAGACATTCACCTTTGAAGGATTTTCTTGACAAGTATGAATTATCTCTTTATAAGAAGTACAAGGAAGAATCTTTAGCAGATATTGAATCTAGAAGCTCTAGCCCCTTGCTGAAAACCAAATGCTCTTTTGAACTGCAGTTGTCTAGCATGTACACAAAAGAAATATTCATGAAGTTCCAACTTGAAGTGGAAGAAATGTTCTCTTGTTTTGGTACCACTCAACTACGTGCAGATGGCTCCATCATAATCTTCTTGGTCAAAGAGCGTGTTTTGATTGAGGGAAACAGACAGGAGATAAAGGATTTTGAAGTTGTTTACAGTAAATCAGCATGTGAAGTCCGTTGTATTTGTTCTTGCTTTAATTTTTATGGATATCTATGTCGGCATGCGCTGTGTGTACTCAATTTTAATGGGGTGGAAGAGATTCCTCCTAAGTACATTCTATCACGGTGGAAGAAAGATTACAAACGCCTTTATATTCCTGATCACAACTCTGGTAGTTCTGATGATATTGAACGTATTCAATGGTCTAATCAGTTATTTGGAAGTGCCTTACAAGTTATGGAGGAGGGAATGATTTCTATAGACCATTACGATGCAGCGCGTCAGGCTTTTGAAGAATCACTTAATAAGGTTCATGATGTAAAACTTGAGAGGTGA
Protein sequence

>MS.gene02021.t1

MMSELQTQKLDTVDGESQPQEEEQEHPKVDDISVCCDQLPDGECQVIELDCQDDISEGRKEFPAPALGMEFESYDDAYSYYICYAKEVGFCVRIKNSCFKRNSKEKYGAVLCCSSQGFNRTKDVDILRKETRTGCPAMIRIKLVESQRWRICEVTLEHNHVLGAKIHKLVKKMASGTKKNSLPTSDAEGKTIKIYHALVIDTEGNGNLNSNATDDRAFSKYSNKLNLRKGDSQAIYNFLCRMQLTNPNFFYLMDFNDEGHLRNVLWVDARSKAACRYFSDVIYFDNTYLSNKYEIPLVTFVGINHHGQSVLLGCGLLAGETTESYSWLFRTWVTCMSGCSPQTIITDRCKTLQSAIAENFPRSHHCFGLSLVMKKVPEKLGGLRNYDAIRKALIKAVYETLKVIEFEAAWGFMIQHFEVRDNEWLLSLYEDRVHWAPVYIKDKFLGGMATTRPSKSISPFFDKYMFRHSPLKDFLDKYELSLYKKYKEESLADIESRSSSPLLKTKCSFELQLSSMYTKEIFMKFQLEVEEMFSCFGTTQLRADGSIIIFLVKERVLIEGNRQEIKDFEVVYSKSACEVRCICSCFNFYGYLCRHALCVLNFNGVEEIPPKYILSRWKKDYKRLYIPDHNSGSSDDIERIQWSNQLFGSALQVMEEGMISIDHYDAARQAFEESLNKVHDVKLER