AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020349

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020349.t1 MTR_7g096530 98.165 218 4 0 1 218 1 218 7.27e-164 451
MS.gene020349.t1 MTR_1g070220 59.649 228 59 10 1 218 1 205 4.85e-79 236
MS.gene020349.t1 MTR_7g096610 70.796 113 33 0 9 121 3 115 6.03e-55 175
MS.gene020349.t1 MTR_1g070205 71.429 105 30 0 14 118 12 116 2.47e-52 169
MS.gene020349.t1 MTR_8g036105 67.857 112 36 0 5 116 9 120 1.10e-50 164
MS.gene020349.t1 MTR_8g036085 69.903 103 31 0 14 116 23 125 9.54e-50 162
MS.gene020349.t1 MTR_6g027710 67.961 103 33 0 13 115 39 141 1.13e-46 154
MS.gene020349.t1 MTR_5g080470 64.000 100 36 0 9 108 2 101 1.85e-45 150
MS.gene020349.t1 MTR_7g074990 66.667 102 34 0 14 115 20 121 4.96e-45 150
MS.gene020349.t1 MTR_7g075230 65.487 113 37 2 6 116 4 116 2.40e-44 147
MS.gene020349.t1 MTR_2g068760 56.250 112 48 1 6 117 5 115 5.79e-42 140
MS.gene020349.t1 MTR_4g083680 47.500 160 74 5 1 159 1 151 5.06e-41 138
MS.gene020349.t1 MTR_7g033800 60.000 115 42 2 1 115 1 111 1.95e-40 136
MS.gene020349.t1 MTR_5g083230 56.604 106 45 1 10 115 2 106 1.33e-38 131
MS.gene020349.t1 MTR_3g077240 57.273 110 46 1 6 115 2 110 2.67e-38 131
MS.gene020349.t1 MTR_3g071590 51.613 124 58 2 11 133 5 127 6.35e-38 129
MS.gene020349.t1 MTR_4g105170 63.542 96 34 1 13 108 10 104 1.41e-37 129
MS.gene020349.t1 MTR_3g031660 56.075 107 45 2 10 115 2 107 1.92e-37 129
MS.gene020349.t1 MTR_6g005070 56.881 109 46 1 7 115 2 109 2.54e-37 127
MS.gene020349.t1 MTR_5g083960 53.333 105 48 1 11 115 36 139 2.45e-36 126
MS.gene020349.t1 MTR_6g027700 60.396 101 40 0 14 114 7 107 3.07e-36 127
MS.gene020349.t1 MTR_5g017950 55.357 112 49 1 8 119 2 112 5.79e-36 129
MS.gene020349.t1 MTR_5g083010 51.327 113 54 1 5 117 2 113 9.95e-36 124
MS.gene020349.t1 MTR_6g005080 54.128 109 49 1 7 115 3 110 5.13e-35 121
MS.gene020349.t1 MTR_3g452660 54.630 108 48 1 9 116 2 108 2.71e-34 124
MS.gene020349.t1 MTR_3g073690 55.046 109 48 1 8 116 3 110 3.93e-34 121
MS.gene020349.t1 MTR_3g094690 53.333 105 48 1 11 115 44 147 5.77e-34 121
MS.gene020349.t1 MTR_3g071420 50.000 112 55 1 1 112 1 111 7.86e-34 120
MS.gene020349.t1 MTR_5g075020 56.250 112 48 1 4 115 33 143 9.42e-34 120
MS.gene020349.t1 MTR_5g075020 56.250 112 48 1 4 115 37 147 1.79e-33 120
MS.gene020349.t1 MTR_4g060950 50.000 114 55 2 3 115 13 125 2.45e-33 119
MS.gene020349.t1 MTR_2g093310 52.427 103 48 1 13 115 32 133 4.14e-33 118
MS.gene020349.t1 MTR_8g040900 56.897 116 42 2 1 116 1 108 6.21e-33 119
MS.gene020349.t1 MTR_3g094690 53.333 105 47 2 11 115 44 146 3.63e-32 117
MS.gene020349.t1 MTR_7g028905 43.363 113 63 1 13 125 4 115 4.84e-30 108
MS.gene020349.t1 MTR_5g093010 42.742 124 69 2 15 137 6 128 1.55e-29 107
MS.gene020349.t1 MTR_1g095850 50.495 101 49 1 15 115 73 172 2.93e-29 109
MS.gene020349.t1 MTR_8g079660 43.689 103 57 1 14 116 8 109 1.60e-23 95.1
MS.gene020349.t1 MTR_4g107450 41.509 106 61 1 10 115 6 110 3.70e-23 94.7
MS.gene020349.t1 MTR_8g079620 41.748 103 59 1 14 116 8 109 5.90e-21 87.8
MS.gene020349.t1 MTR_8g017090 40.566 106 62 1 11 116 9 113 1.85e-20 87.8
MS.gene020349.t1 MTR_2g100020 37.383 107 66 1 10 116 15 120 4.13e-20 86.3
MS.gene020349.t1 MTR_6g011230 32.824 131 87 1 11 141 8 137 2.77e-19 82.4
MS.gene020349.t1 MTR_6g011250 30.645 186 117 6 15 199 10 184 1.69e-18 80.1
MS.gene020349.t1 MTR_6g011200 35.780 109 68 2 15 122 10 117 2.07e-17 78.6
MS.gene020349.t1 MTR_8g079580 39.806 103 60 2 14 115 8 109 4.33e-17 77.4
MS.gene020349.t1 MTR_4g099230 35.780 109 68 2 15 122 3 110 7.46e-16 72.4
MS.gene020349.t1 MTR_5g085390 33.654 104 67 2 15 117 10 112 6.31e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020349.t1 AT2G42430 55.422 249 76 9 1 218 1 245 1.61e-79 238
MS.gene020349.t1 AT3G58190 62.205 127 40 1 14 132 11 137 1.63e-53 171
MS.gene020349.t1 AT3G03760 76.699 103 24 0 13 115 50 152 1.09e-51 168
MS.gene020349.t1 AT4G00210 66.964 112 37 0 5 116 2 113 2.01e-51 166
MS.gene020349.t1 AT2G45420 76.000 100 24 0 17 116 40 139 1.84e-50 165
MS.gene020349.t1 AT2G42440 61.600 125 40 1 9 125 2 126 1.94e-50 164
MS.gene020349.t1 AT2G31310 61.404 114 44 0 10 123 3 116 3.18e-50 162
MS.gene020349.t1 AT5G06080 60.331 121 47 1 12 132 5 124 1.56e-47 154
MS.gene020349.t1 AT4G00220 69.000 100 31 0 17 116 20 119 1.30e-45 151
MS.gene020349.t1 AT2G45410 68.000 100 32 0 17 116 19 118 8.12e-45 148
MS.gene020349.t1 AT5G63090 58.120 117 45 2 1 117 1 113 3.60e-39 133
MS.gene020349.t1 AT5G63090 58.120 117 45 2 1 117 1 113 3.60e-39 133
MS.gene020349.t1 AT5G63090 58.120 117 45 2 1 117 1 113 3.60e-39 133
MS.gene020349.t1 AT5G63090 58.120 117 45 2 1 117 1 113 3.60e-39 133
MS.gene020349.t1 AT3G27650 56.481 108 46 1 8 115 33 139 3.63e-38 130
MS.gene020349.t1 AT2G30130 57.009 107 44 2 10 115 3 108 8.14e-37 127
MS.gene020349.t1 AT1G31320 58.696 92 37 1 10 101 9 99 4.49e-36 125
MS.gene020349.t1 AT2G30340 46.970 132 68 2 1 131 39 169 5.75e-36 127
MS.gene020349.t1 AT2G30340 46.970 132 68 2 1 131 40 170 7.21e-36 127
MS.gene020349.t1 AT1G07900 53.398 103 47 1 13 115 32 133 1.53e-34 122
MS.gene020349.t1 AT5G66870 39.884 173 88 4 9 180 2 159 7.90e-34 123
MS.gene020349.t1 AT2G28500 53.398 103 47 1 13 115 54 155 1.01e-33 121
MS.gene020349.t1 AT3G11090 46.457 127 66 2 6 131 3 128 1.72e-33 118
MS.gene020349.t1 AT1G65620 59.091 110 44 1 7 116 2 110 9.23e-33 117
MS.gene020349.t1 AT1G65620 59.091 110 44 1 7 116 2 110 9.23e-33 117
MS.gene020349.t1 AT1G65620 59.091 110 44 1 7 116 2 110 9.23e-33 117
MS.gene020349.t1 AT1G65620 59.091 110 44 1 7 116 2 110 9.23e-33 117
MS.gene020349.t1 AT1G65620 59.091 110 44 1 7 116 2 110 9.23e-33 117
MS.gene020349.t1 AT1G16530 51.852 108 50 2 11 117 11 117 9.86e-33 116
MS.gene020349.t1 AT2G23660 50.485 103 50 1 13 115 4 105 1.97e-31 117
MS.gene020349.t1 AT2G23660 50.485 103 50 1 13 115 4 105 1.97e-31 117
MS.gene020349.t1 AT2G23660 50.485 103 50 1 13 115 4 105 1.97e-31 117
MS.gene020349.t1 AT2G40470 56.190 105 45 1 11 115 17 120 3.45e-30 110
MS.gene020349.t1 AT2G40470 56.190 105 45 1 11 115 42 145 5.29e-30 111
MS.gene020349.t1 AT3G26660 44.118 102 56 1 15 116 6 106 5.29e-29 105
MS.gene020349.t1 AT3G26620 44.118 102 56 1 15 116 6 106 8.49e-29 105
MS.gene020349.t1 AT3G50510 46.667 105 55 1 12 116 10 113 1.41e-24 96.3
MS.gene020349.t1 AT3G50510 46.667 105 55 1 12 116 10 113 1.41e-24 96.3
MS.gene020349.t1 AT3G47870 38.679 106 64 1 10 115 32 136 1.67e-20 88.2
MS.gene020349.t1 AT3G13850 42.157 102 58 1 15 116 37 137 2.60e-20 86.7
MS.gene020349.t1 AT5G35900 38.532 109 66 1 15 123 6 113 4.11e-18 79.7
MS.gene020349.t1 AT1G72980 37.037 108 67 1 8 115 7 113 2.04e-17 77.8
MS.gene020349.t1 AT1G06280 41.772 79 37 2 15 89 25 98 3.09e-14 69.3
MS.gene020349.t1 AT4G22700 42.529 87 45 3 15 100 6 88 1.94e-12 63.9

Find 31 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATGTAAGTTGGACAAAA+TGG 0.286192 7.2:+13727048 None:intergenic
AGTGAACTTTGAGGAGATAT+AGG 0.327596 7.2:+13727024 None:intergenic
TGAATAGCTGCAAATCTTGC+AGG 0.329259 7.2:+13727885 None:intergenic
ATCAATATGGCTTCTTCAAA+TGG 0.350219 7.2:-13728011 None:intergenic
GCAGCTATTCATAAAGTGTT+TGG 0.364830 7.2:-13727873 MS.gene020349:CDS
TGTTGTCACAATTGCTTATG+AGG 0.389067 7.2:-13727793 MS.gene020349:CDS
CGTATTAGAGACCCTGTATA+TGG 0.390387 7.2:-13727762 MS.gene020349:CDS
GTGAACTTTGAGGAGATATA+GGG 0.398352 7.2:+13727025 None:intergenic
TATGCAGGTAGCATGCTTGC+AGG 0.403290 7.2:-13727175 MS.gene020349:intron
GGAAACAATAATAATGTTGC+TGG 0.425208 7.2:-13727090 MS.gene020349:CDS
GATATAGGGTTCATGTAAGT+TGG 0.426071 7.2:+13727039 None:intergenic
GATGACTCAAGTGAACTTTG+AGG 0.443078 7.2:+13727015 None:intergenic
TGAGAGACACAGCCATATAC+AGG 0.444019 7.2:+13727750 None:intergenic
TCTTCAAATGGAACTAGCTC+TGG 0.444575 7.2:-13727999 MS.gene020349:CDS
TCTTATAATTATTACAACCT+TGG 0.449925 7.2:+13726914 None:intergenic
CAATAATAATGATCATGACT+TGG 0.461238 7.2:-13726875 MS.gene020349:CDS
GAGAATAATAATCAATGGAC+AGG 0.471919 7.2:-13727111 MS.gene020349:CDS
ATTGTGTACCTGTTGTTGTA+AGG 0.501180 7.2:+13727719 None:intergenic
CATAGCAGCAGCATGAATGA+TGG 0.514545 7.2:-13726985 MS.gene020349:CDS
ACATAGAGAATAATAATCAA+TGG 0.518649 7.2:-13727116 MS.gene020349:CDS
CCTTGTTCTGAGCAAAAGTA+AGG 0.518854 7.2:+13727909 None:intergenic
AATGGAACTAGCTCTGGAAC+TGG 0.534733 7.2:-13727993 MS.gene020349:CDS
AGAGAAGATTTCTCATTCCA+AGG 0.536983 7.2:-13726931 MS.gene020349:CDS
AATAATAATGATCATGACTT+GGG 0.555968 7.2:-13726874 MS.gene020349:CDS
GCTTCACAACGTTCATGAGC+TGG 0.566405 7.2:+13727816 None:intergenic
TCTGGAACTGGTTCTCCTTG+TGG 0.567515 7.2:-13727981 MS.gene020349:CDS
AAATAGCACTTAGGATGATG+AGG 0.594424 7.2:-13726840 MS.gene020349:CDS
GAATAGCTGCAAATCTTGCA+GGG 0.610026 7.2:+13727886 None:intergenic
AGGAACTTGCATGCTCCACA+AGG 0.611585 7.2:+13727966 None:intergenic
AATTGCAAGAAATAGCACTT+AGG 0.616097 7.2:-13726849 MS.gene020349:CDS
GAGAGACACAGCCATATACA+GGG 0.671769 7.2:+13727751 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATATTAAAAAAATAA+AGG + chr7.2:13727429-13727448 None:intergenic 0.0%
!! ATATATATTAAAAAAATAAA+GGG + chr7.2:13727428-13727447 None:intergenic 0.0%
!! TTTAATATATATTAGAAATG+TGG - chr7.2:13727436-13727455 MS.gene020349:intron 10.0%
!! AATAAATAATATTCTTTGAC+TGG + chr7.2:13727530-13727549 None:intergenic 15.0%
!! AATTATATAAACTTCACATT+AGG - chr7.2:13727149-13727168 MS.gene020349:CDS 15.0%
!! AATAATAATGATCATGACTT+GGG - chr7.2:13727965-13727984 MS.gene020349:CDS 20.0%
!! ACATAGAGAATAATAATCAA+TGG - chr7.2:13727723-13727742 MS.gene020349:intron 20.0%
!! TCTTATAATTATTACAACCT+TGG + chr7.2:13727928-13727947 None:intergenic 20.0%
!!! AGTTTTCTTTTTAATTATGC+AGG - chr7.2:13727649-13727668 MS.gene020349:intron 20.0%
! AGTACCAATAGAATTCATTA+AGG + chr7.2:13727620-13727639 None:intergenic 25.0%
! ATCACCTTAATGAATTCTAT+TGG - chr7.2:13727613-13727632 MS.gene020349:intron 25.0%
! CAATAATAATGATCATGACT+TGG - chr7.2:13727964-13727983 MS.gene020349:CDS 25.0%
!!! AGTTCTAAAAAAAACTTTGG+TGG + chr7.2:13727374-13727393 None:intergenic 25.0%
!!! GCAAGTTCTAAAAAAAACTT+TGG + chr7.2:13727377-13727396 None:intergenic 25.0%
!!! TTCTAATTAAAGGTGTTGTT+AGG - chr7.2:13727263-13727282 MS.gene020349:intron 25.0%
AATTGCAAGAAATAGCACTT+AGG - chr7.2:13727990-13728009 MS.gene020349:CDS 30.0%
GAGAATAATAATCAATGGAC+AGG - chr7.2:13727728-13727747 MS.gene020349:CDS 30.0%
GCACTAACACTTCTAATTAA+AGG - chr7.2:13727253-13727272 MS.gene020349:intron 30.0%
GGAAACAATAATAATGTTGC+TGG - chr7.2:13727749-13727768 MS.gene020349:CDS 30.0%
! TTCATGTAAGTTGGACAAAA+TGG + chr7.2:13727794-13727813 None:intergenic 30.0%
AAATAGCACTTAGGATGATG+AGG - chr7.2:13727999-13728018 MS.gene020349:CDS 35.0%
AAGAGCCTTAAGAAAAAGAC+AGG + chr7.2:13727493-13727512 None:intergenic 35.0%
AGAGAAGATTTCTCATTCCA+AGG - chr7.2:13727908-13727927 MS.gene020349:CDS 35.0%
ATGTGATCAATCACATGTGT+AGG + chr7.2:13727312-13727331 None:intergenic 35.0%
ATTGTGTACCTGTTGTTGTA+AGG + chr7.2:13727123-13727142 None:intergenic 35.0%
GATATAGGGTTCATGTAAGT+TGG + chr7.2:13727803-13727822 None:intergenic 35.0%
TAAGGTGCAAAAATGCAATC+AGG + chr7.2:13726915-13726934 None:intergenic 35.0%
TGTTGTCACAATTGCTTATG+AGG - chr7.2:13727046-13727065 MS.gene020349:CDS 35.0%
! AGTGAACTTTGAGGAGATAT+AGG + chr7.2:13727818-13727837 None:intergenic 35.0%
! CATTTTTGCCTTACAACAAC+AGG - chr7.2:13727112-13727131 MS.gene020349:CDS 35.0%
! CTACTCCTGTCTTTTTCTTA+AGG - chr7.2:13727485-13727504 MS.gene020349:intron 35.0%
! GCAGCTATTCATAAAGTGTT+TGG - chr7.2:13726966-13726985 MS.gene020349:CDS 35.0%
! GTGAACTTTGAGGAGATATA+GGG + chr7.2:13727817-13727836 None:intergenic 35.0%
AAGGCTCTTTGATGCTTCAA+GGG - chr7.2:13727504-13727523 MS.gene020349:intron 40.0%
ACTACACTTACTCACGTATC+TGG - chr7.2:13727231-13727250 MS.gene020349:intron 40.0%
AGAAAAAGACAGGAGTAGTG+TGG + chr7.2:13727483-13727502 None:intergenic 40.0%
CGTATTAGAGACCCTGTATA+TGG - chr7.2:13727077-13727096 MS.gene020349:CDS 40.0%
GAATAGCTGCAAATCTTGCA+GGG + chr7.2:13726956-13726975 None:intergenic 40.0%
GATGACTCAAGTGAACTTTG+AGG + chr7.2:13727827-13727846 None:intergenic 40.0%
TAAGGCTCTTTGATGCTTCA+AGG - chr7.2:13727503-13727522 MS.gene020349:intron 40.0%
TCTTCAAATGGAACTAGCTC+TGG - chr7.2:13726840-13726859 MS.gene020349:CDS 40.0%
TGAATAGCTGCAAATCTTGC+AGG + chr7.2:13726957-13726976 None:intergenic 40.0%
! CAGGTGCACATTTTCTTCTA+AGG + chr7.2:13726896-13726915 None:intergenic 40.0%
! CCTTGTTCTGAGCAAAAGTA+AGG + chr7.2:13726933-13726952 None:intergenic 40.0%
!! CCTTACTTTTGCTCAGAACA+AGG - chr7.2:13726930-13726949 MS.gene020349:CDS 40.0%
CATAGCAGCAGCATGAATGA+TGG - chr7.2:13727854-13727873 MS.gene020349:CDS 45.0%
GAGAGACACAGCCATATACA+GGG + chr7.2:13727091-13727110 None:intergenic 45.0%
TGAGAGACACAGCCATATAC+AGG + chr7.2:13727092-13727111 None:intergenic 45.0%
!! AATGGAACTAGCTCTGGAAC+TGG - chr7.2:13726846-13726865 MS.gene020349:CDS 45.0%
!! TATATATTAAAAAAATAAAG+GGG + chr7.2:13727427-13727446 None:intergenic 5.0%
AGGAACTTGCATGCTCCACA+AGG + chr7.2:13726876-13726895 None:intergenic 50.0%
GCTTCACAACGTTCATGAGC+TGG + chr7.2:13727026-13727045 None:intergenic 50.0%
TATGCAGGTAGCATGCTTGC+AGG - chr7.2:13727664-13727683 MS.gene020349:intron 50.0%
! TCTGGAACTGGTTCTCCTTG+TGG - chr7.2:13726858-13726877 MS.gene020349:CDS 50.0%
GTTAGGTGTTCGACACGTGC+AGG - chr7.2:13727280-13727299 MS.gene020349:intron 55.0%
AGGTGTTCGACACGTGCAGG+CGG - chr7.2:13727283-13727302 MS.gene020349:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 13726834 13728027 13726834 ID=MS.gene020349
chr7.2 mRNA 13726834 13728027 13726834 ID=MS.gene020349.t1;Parent=MS.gene020349
chr7.2 exon 13727728 13728027 13727728 ID=MS.gene020349.t1.exon1;Parent=MS.gene020349.t1
chr7.2 CDS 13727728 13728027 13727728 ID=cds.MS.gene020349.t1;Parent=MS.gene020349.t1
chr7.2 exon 13726834 13727190 13726834 ID=MS.gene020349.t1.exon2;Parent=MS.gene020349.t1
chr7.2 CDS 13726834 13727190 13726834 ID=cds.MS.gene020349.t1;Parent=MS.gene020349.t1
Gene Sequence

>MS.gene020349

ATGGCTTCTTCAAATGGAACTAGCTCTGGAACTGGTTCTCCTTGTGGAGCATGCAAGTTCCTTAGAAGAAAATGTGCACCTGATTGCATTTTTGCACCTTACTTTTGCTCAGAACAAGGCCCTGCAAGATTTGCAGCTATTCATAAAGTGTTTGGTGCTAGCAATGTTTCTAAGTTGCTTTTGCATATACCAGCTCATGAACGTTGTGAAGCTGTTGTCACAATTGCTTATGAGGCTCAAGCTCGTATTAGAGACCCTGTATATGGCTGTGTCTCTCACATTTTTGCCTTACAACAACAGGTACACAATATAGTTAATTATATAAACTTCACATTAGGCTATGTTTTTTCTGTTAATGAACAATAGAAGTTCATTCAACTTCTAATAACTAATTAACACTACACTTACTCACGTATCTGGCACTAACACTTCTAATTAAAGGTGTTGTTAGGTGTTCGACACGTGCAGGCGGACACCTACACATGTGATTGATCACATTCAATTATTTTCAGTGATTCATAGTTCGTCATTGATCATCCACCAAAGTTTTTTTTAGAACTTGCAATTTTCAATTTTTTTTTTTTATCTCTCCCCTTTATTTTTTTAATATATATTAGAAATGTGGAAGATCATTGAGATAATTAAACCACACTACTCCTGTCTTTTTCTTAAGGCTCTTTGATGCTTCAAGGGCCAGTCAAAGAATATTATTTATTGTCACTATGTATATTAAATGTTCATTAGTCAATTTATTAATAAACAAACAAATTTTCTAGTTTATCACCTTAATGAATTCTATTGGTACTTCTTCTCTAAGTTTTCTTTTTAATTATGCAGGTAGCATGCTTGCAGGCACAATTGATGCAAGTGAAATCTCAACTGTCACATAACATAGAGAATAATAATCAATGGACAGGAAACAATAATAATGTTGCTGGCACACAAGCAATGAATTATCCATTTTGTCCAACTTACATGAACCCTATATCTCCTCAAAGTTCACTTGAGTCATCAATTGATCATAGCAGCAGCATGAATGATGGAATGATGAGCATGCAAGATGTACAAAGTAGCAGAGAAGATTTCTCATTCCAAGGTTGTAATAATTATAAGAAAAGAATGTCACATAACAATAATAATGATCATGACTTGGGTGAATTGCAAGAAATAGCACTTAGGATGATGAGGAACTAA
Protein sequence

>MS.gene020349.t1

MASSNGTSSGTGSPCGACKFLRRKCAPDCIFAPYFCSEQGPARFAAIHKVFGASNVSKLLLHIPAHERCEAVVTIAYEAQARIRDPVYGCVSHIFALQQQVACLQAQLMQVKSQLSHNIENNNQWTGNNNNVAGTQAMNYPFCPTYMNPISPQSSLESSIDHSSSMNDGMMSMQDVQSSREDFSFQGCNNYKKRMSHNNNNDHDLGELQEIALRMMRN