Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020409.t1 | XP_003602944.1 | 90.5 | 253 | 17 | 2 | 1 | 253 | 29 | 274 | 3.10E-63 | 251.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020409.t1 | Q9SB61 | 47.4 | 249 | 88 | 2 | 5 | 253 | 15 | 220 | 6.2e-44 | 179.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020409.t1 | G7J570 | 90.5 | 253 | 17 | 2 | 1 | 253 | 29 | 274 | 2.3e-63 | 251.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020409.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020409.t1 | MTR_3g100650 | 95.257 | 253 | 5 | 2 | 1 | 253 | 29 | 274 | 7.61e-179 | 492 |
| MS.gene020409.t1 | MTR_5g065440 | 63.846 | 260 | 71 | 4 | 6 | 253 | 20 | 268 | 1.23e-98 | 289 |
| MS.gene020409.t1 | MTR_5g032600 | 54.167 | 216 | 70 | 7 | 50 | 253 | 74 | 272 | 5.61e-62 | 196 |
| MS.gene020409.t1 | MTR_4g134580 | 43.515 | 239 | 92 | 6 | 53 | 252 | 112 | 346 | 1.27e-55 | 182 |
| MS.gene020409.t1 | MTR_1g077020 | 44.554 | 202 | 91 | 3 | 51 | 252 | 6 | 186 | 3.01e-53 | 171 |
| MS.gene020409.t1 | MTR_5g020510 | 43.333 | 210 | 105 | 3 | 49 | 252 | 94 | 295 | 4.82e-48 | 161 |
| MS.gene020409.t1 | MTR_2g076780 | 43.541 | 209 | 99 | 5 | 54 | 252 | 37 | 236 | 6.31e-48 | 159 |
| MS.gene020409.t1 | MTR_8g069080 | 42.788 | 208 | 103 | 3 | 53 | 252 | 68 | 267 | 6.11e-47 | 157 |
| MS.gene020409.t1 | MTR_7g010300 | 41.121 | 214 | 98 | 5 | 51 | 252 | 85 | 282 | 3.30e-46 | 157 |
| MS.gene020409.t1 | MTR_6g012280 | 41.463 | 205 | 103 | 5 | 54 | 252 | 91 | 284 | 5.83e-39 | 138 |
| MS.gene020409.t1 | MTR_8g069380 | 51.163 | 86 | 41 | 1 | 168 | 253 | 31 | 115 | 1.43e-21 | 87.0 |
| MS.gene020409.t1 | MTR_3g039810 | 61.290 | 62 | 24 | 0 | 52 | 113 | 18 | 79 | 6.68e-21 | 84.3 |
| MS.gene020409.t1 | MTR_8g051480 | 54.667 | 75 | 33 | 1 | 178 | 252 | 55 | 128 | 7.12e-21 | 85.5 |
| MS.gene020409.t1 | MTR_8g442330 | 34.756 | 164 | 68 | 5 | 64 | 227 | 15 | 139 | 2.60e-20 | 84.3 |
| MS.gene020409.t1 | MTR_7g070643 | 80.435 | 46 | 9 | 0 | 47 | 92 | 75 | 120 | 5.86e-19 | 82.0 |
| MS.gene020409.t1 | MTR_8g467340 | 53.968 | 63 | 29 | 0 | 190 | 252 | 271 | 333 | 1.44e-18 | 84.3 |
| MS.gene020409.t1 | MTR_8g467340 | 56.364 | 55 | 22 | 1 | 51 | 103 | 61 | 115 | 4.16e-13 | 68.6 |
| MS.gene020409.t1 | MTR_6g080300 | 80.952 | 42 | 8 | 0 | 51 | 92 | 51 | 92 | 6.44e-18 | 76.6 |
| MS.gene020409.t1 | MTR_6g073013 | 55.000 | 60 | 26 | 1 | 190 | 248 | 101 | 160 | 5.75e-17 | 76.6 |
| MS.gene020409.t1 | MTR_6g073013 | 52.000 | 50 | 23 | 1 | 54 | 102 | 19 | 68 | 8.32e-11 | 59.7 |
| MS.gene020409.t1 | MTR_6g012220 | 55.769 | 52 | 19 | 1 | 51 | 102 | 18 | 65 | 4.00e-13 | 63.5 |
| MS.gene020409.t1 | MTR_2g007810 | 93.103 | 29 | 2 | 0 | 64 | 92 | 19 | 47 | 1.92e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020409.t1 | AT4G24660 | 53.202 | 203 | 65 | 5 | 52 | 253 | 47 | 220 | 2.47e-66 | 205 |
| MS.gene020409.t1 | AT4G24660 | 52.261 | 199 | 65 | 5 | 52 | 249 | 47 | 216 | 4.22e-63 | 197 |
| MS.gene020409.t1 | AT2G02540 | 42.081 | 221 | 105 | 5 | 43 | 252 | 76 | 284 | 3.93e-60 | 192 |
| MS.gene020409.t1 | AT2G02540 | 42.081 | 221 | 105 | 5 | 43 | 252 | 76 | 284 | 3.93e-60 | 192 |
| MS.gene020409.t1 | AT1G14440 | 45.366 | 205 | 96 | 4 | 52 | 252 | 88 | 280 | 1.86e-54 | 178 |
| MS.gene020409.t1 | AT1G14440 | 45.366 | 205 | 96 | 4 | 52 | 252 | 88 | 280 | 1.86e-54 | 178 |
| MS.gene020409.t1 | AT5G65410 | 45.536 | 224 | 74 | 5 | 44 | 253 | 65 | 254 | 5.03e-51 | 168 |
| MS.gene020409.t1 | AT2G18350 | 47.291 | 203 | 87 | 4 | 50 | 252 | 78 | 260 | 1.82e-50 | 166 |
| MS.gene020409.t1 | AT3G50890 | 43.216 | 199 | 98 | 3 | 53 | 251 | 59 | 242 | 1.56e-46 | 155 |
| MS.gene020409.t1 | AT1G75240 | 41.126 | 231 | 108 | 2 | 51 | 253 | 73 | 303 | 4.48e-46 | 156 |
| MS.gene020409.t1 | AT5G15210 | 38.863 | 211 | 100 | 5 | 44 | 246 | 46 | 235 | 5.86e-38 | 134 |
| MS.gene020409.t1 | AT1G69600 | 40.291 | 206 | 96 | 7 | 52 | 249 | 29 | 215 | 8.35e-37 | 130 |
| MS.gene020409.t1 | AT3G28920 | 33.333 | 231 | 92 | 4 | 52 | 249 | 50 | 251 | 3.97e-34 | 125 |
| MS.gene020409.t1 | AT5G60480 | 37.931 | 203 | 88 | 4 | 51 | 247 | 34 | 204 | 1.23e-32 | 119 |
| MS.gene020409.t1 | AT1G74660 | 69.355 | 62 | 19 | 0 | 52 | 113 | 37 | 98 | 1.33e-24 | 94.7 |
| MS.gene020409.t1 | AT5G42780 | 31.553 | 206 | 100 | 5 | 54 | 249 | 64 | 238 | 1.49e-24 | 98.6 |
| MS.gene020409.t1 | AT1G74660 | 72.414 | 58 | 16 | 0 | 52 | 109 | 37 | 94 | 2.68e-24 | 93.6 |
| MS.gene020409.t1 | AT3G28917 | 66.667 | 60 | 19 | 1 | 51 | 109 | 30 | 89 | 8.24e-22 | 87.4 |
| MS.gene020409.t1 | AT1G18835 | 60.317 | 63 | 22 | 1 | 51 | 113 | 23 | 82 | 1.13e-19 | 81.3 |
| MS.gene020409.t1 | AT5G39760 | 53.846 | 65 | 30 | 0 | 188 | 252 | 198 | 262 | 2.46e-19 | 85.9 |
| MS.gene020409.t1 | AT1G14687 | 26.866 | 201 | 104 | 7 | 50 | 246 | 3 | 164 | 4.99e-14 | 68.6 |
Find 0 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATTCACACAGGAACAAA+AGG | + | 51443:181201-181220 | MS.gene020409:CDS | 30.0% | |
| ! | GTTTAGAACAAAATTCACAC+AGG | + | 51443:181192-181211 | MS.gene020409:CDS | 30.0% |
| AGAATTCAGAAGGAAGATGA+AGG | + | 51443:181259-181278 | MS.gene020409:CDS | 35.0% | |
| AGTAAGGTGATAATTGATGG+TGG | - | 51443:180976-180995 | None:intergenic | 35.0% | |
| GGTAGTAAGGTGATAATTGA+TGG | - | 51443:180979-180998 | None:intergenic | 35.0% | |
| TATCAAGAGACATGTGCTTA+AGG | + | 51443:181312-181331 | MS.gene020409:CDS | 35.0% | |
| TTGCATTTGCGGAGAAAATT+GGG | + | 51443:181233-181252 | MS.gene020409:CDS | 35.0% | |
| AACGGACGATGTTGTGATAT+CGG | - | 51443:181055-181074 | None:intergenic | 40.0% | |
| AATTGGGTGGAGAATTCAGA+AGG | + | 51443:181249-181268 | MS.gene020409:CDS | 40.0% | |
| AGAGACATGTGCTTAAGGTT+TGG | + | 51443:181317-181336 | MS.gene020409:CDS | 40.0% | |
| CACAATAACAAGCACACTCT+TGG | + | 51443:181343-181362 | MS.gene020409:CDS | 40.0% | |
| CTTGCATTTGCGGAGAAAAT+TGG | + | 51443:181232-181251 | MS.gene020409:CDS | 40.0% | |
| GGATAAGATGCTTGCATTTG+CGG | + | 51443:181222-181241 | MS.gene020409:CDS | 40.0% | |
| TCAGGAGTTAGGTCCAAAAA+TGG | + | 51443:180665-180684 | MS.gene020409:CDS | 40.0% | |
| TTGTGATATCGGTGGAGTTA+CGG | - | 51443:181044-181063 | None:intergenic | 40.0% | |
| TTTCACCGCAAAGAAATCGA+CGG | + | 51443:180911-180930 | MS.gene020409:CDS | 40.0% | |
| TTTCTTTGCGGTGAAAGTTC+CGG | - | 51443:180907-180926 | None:intergenic | 40.0% | |
| !! | AAGGTGATAATTGATGGTGG+TGG | - | 51443:180973-180992 | None:intergenic | 40.0% |
| !! | ATTCACCGTCGATTTCTTTG+CGG | - | 51443:180919-180938 | None:intergenic | 40.0% |
| AAACTCGCAACATCCATCCA+CGG | - | 51443:180825-180844 | None:intergenic | 45.0% | |
| AGAAGAAGGAACACTGGAAG+CGG | + | 51443:180856-180875 | MS.gene020409:CDS | 45.0% | |
| CATTTGCGGAGAAAATTGGG+TGG | + | 51443:181236-181255 | MS.gene020409:CDS | 45.0% | |
| GAGGAAGTGGAACGAAGAAA+AGG | + | 51443:181170-181189 | MS.gene020409:CDS | 45.0% | |
| GATATCACAACATCGTCCGT+TGG | + | 51443:181054-181073 | MS.gene020409:CDS | 45.0% | |
| GGAGTTAGGTCCAAAAATGG+CGG | + | 51443:180668-180687 | MS.gene020409:CDS | 45.0% | |
| TCCAAAAATGGCGGTGGAAT+CGG | + | 51443:180677-180696 | MS.gene020409:CDS | 45.0% | |
| TTCCAGTGTTCCTTCTTCTC+CGG | - | 51443:180855-180874 | None:intergenic | 45.0% | |
| !! | AATTGATGGTGGTGGTGTTG+AGG | - | 51443:180965-180984 | None:intergenic | 45.0% |
| !! | ATTGATGGTGGTGGTGTTGA+GGG | - | 51443:180964-180983 | None:intergenic | 45.0% |
| !!! | TGTTGCGAGTTTTTAGCTGC+CGG | + | 51443:180833-180852 | MS.gene020409:CDS | 45.0% |
| !!! | TTTTTAGCTGCCGGAGAAGA+AGG | + | 51443:180842-180861 | MS.gene020409:CDS | 45.0% |
| AAGAATCACGCCGTCAGCAT+AGG | + | 51443:180791-180810 | MS.gene020409:CDS | 50.0% | |
| ATATCGGTGGAGTTACGGAG+TGG | - | 51443:181039-181058 | None:intergenic | 50.0% | |
| CGCTTGGAAACTCAGGAGTT+AGG | + | 51443:180654-180673 | MS.gene020409:CDS | 50.0% | |
| GGACGATGTTGTGATATCGG+TGG | - | 51443:181052-181071 | None:intergenic | 50.0% | |
| GTGTCAAATCCAAGCAGCAG+CGG | + | 51443:181127-181146 | MS.gene020409:CDS | 50.0% | |
| GTTAGGTCCAAAAATGGCGG+TGG | + | 51443:180671-180690 | MS.gene020409:CDS | 50.0% | |
| TACGACTCGCTTGGAAACTC+AGG | + | 51443:180647-180666 | MS.gene020409:CDS | 50.0% | |
| TTGTATCTGACCGTTCCTGC+TGG | - | 51443:180761-180780 | None:intergenic | 50.0% | |
| !! | ACCGATTCCACCGCCATTTT+TGG | - | 51443:180681-180700 | None:intergenic | 50.0% |
| !! | GTGCTGCTTGTAACTGTCAC+CGG | + | 51443:180885-180904 | MS.gene020409:CDS | 50.0% |
| AACATGCCACCGGCGTAACA+GGG | - | 51443:180628-180647 | None:intergenic | 55.0% | |
| AACGCTGCACGGTGGTAGTA+AGG | - | 51443:180992-181011 | None:intergenic | 55.0% | |
| AATCACGCCGTCAGCATAGG+TGG | + | 51443:180794-180813 | MS.gene020409:CDS | 55.0% | |
| AATCCCCTACTCGATGCAGC+TGG | - | 51443:181088-181107 | None:intergenic | 55.0% | |
| ACGGTGAATTCACTCCCCAG+CGG | + | 51443:180930-180949 | MS.gene020409:CDS | 55.0% | |
| AGCGAGTCGTAACATGCCAC+CGG | - | 51443:180638-180657 | None:intergenic | 55.0% | |
| CAAAAGTGGGCCAGCAGGAA+CGG | + | 51443:180748-180767 | MS.gene020409:CDS | 55.0% | |
| GTGGCATGTTACGACTCGCT+TGG | + | 51443:180638-180657 | MS.gene020409:CDS | 55.0% | |
| TAACATGCCACCGGCGTAAC+AGG | - | 51443:180629-180648 | None:intergenic | 55.0% | |
| TCAAATCCAAGCAGCAGCGG+TGG | + | 51443:181130-181149 | MS.gene020409:CDS | 55.0% | |
| TCCACCAGCTGCATCGAGTA+GGG | + | 51443:181081-181100 | MS.gene020409:CDS | 55.0% | |
| TGCCGGAGAAGAAGGAACAC+TGG | + | 51443:180850-180869 | MS.gene020409:CDS | 55.0% | |
| TTCCACCAGCTGCATCGAGT+AGG | + | 51443:181080-181099 | MS.gene020409:CDS | 55.0% | |
| TTGAGGGTGATGAGACCGCT+GGG | - | 51443:180948-180967 | None:intergenic | 55.0% | |
| ! | ACAGGCGGTGGTGTCAAAAG+TGG | + | 51443:180734-180753 | MS.gene020409:CDS | 55.0% |
| ! | ACTTTTGACACCACCGCCTG+TGG | - | 51443:180735-180754 | None:intergenic | 55.0% |
| ! | CAGGCGGTGGTGTCAAAAGT+GGG | + | 51443:180735-180754 | MS.gene020409:CDS | 55.0% |
| AATCCAAGCAGCAGCGGTGG+TGG | + | 51443:181133-181152 | MS.gene020409:CDS | 60.0% | |
| ATAGGTGGTCACGCCGTGGA+TGG | + | 51443:180809-180828 | MS.gene020409:CDS | 60.0% | |
| CCACCAGCTGCATCGAGTAG+GGG | + | 51443:181082-181101 | MS.gene020409:CDS | 60.0% | |
| GCAGCTGGTGGAAGAGCCAA+CGG | - | 51443:181073-181092 | None:intergenic | 60.0% | |
| GGCGTGACCACCTATGCTGA+CGG | - | 51443:180804-180823 | None:intergenic | 60.0% | |
| GGTGTCAAAAGTGGGCCAGC+AGG | + | 51443:180743-180762 | MS.gene020409:CDS | 60.0% | |
| GTGGAGTTACGGAGTGGTGG+TGG | - | 51443:181033-181052 | None:intergenic | 60.0% | |
| GTTGAGGGTGATGAGACCGC+TGG | - | 51443:180949-180968 | None:intergenic | 60.0% | |
| TCGGTGGAGTTACGGAGTGG+TGG | - | 51443:181036-181055 | None:intergenic | 60.0% | |
| TGAGGGTGATGAGACCGCTG+GGG | - | 51443:180947-180966 | None:intergenic | 60.0% | |
| TTTGACACCACCGCCTGTGG+CGG | - | 51443:180732-180751 | None:intergenic | 60.0% | |
| ! | GGTGGAATCGGTTCAGCGGA+CGG | + | 51443:180689-180708 | MS.gene020409:CDS | 60.0% |
| ! | TGGCGGTGGAATCGGTTCAG+CGG | + | 51443:180685-180704 | MS.gene020409:CDS | 60.0% |
| !! | ACGGAGTGGTGGTGGAAACC+AGG | - | 51443:181025-181044 | None:intergenic | 60.0% |
| !! | GAGTGGTGGTGGAAACCAGG+CGG | - | 51443:181022-181041 | None:intergenic | 60.0% |
| !! | GGTGGTAGTGGAGGAGGAAG+TGG | + | 51443:181157-181176 | MS.gene020409:CDS | 60.0% |
| CAGCATAGGTGGTCACGCCG+TGG | + | 51443:180805-180824 | MS.gene020409:CDS | 65.0% | |
| CCCCTACTCGATGCAGCTGG+TGG | - | 51443:181085-181104 | None:intergenic | 65.0% | |
| GAGGAGGTAACGCTGCACGG+TGG | - | 51443:181000-181019 | None:intergenic | 65.0% | |
| GCGGAGGAGGTAACGCTGCA+CGG | - | 51443:181003-181022 | None:intergenic | 65.0% | |
| TGGTGGAAACCAGGCGGAGG+AGG | - | 51443:181016-181035 | None:intergenic | 65.0% | |
| ! | GGAATCGGTTCAGCGGACGG+AGG | + | 51443:180692-180711 | MS.gene020409:CDS | 65.0% |
| !! | AGCGGTGGTGGTGGTGGTAG+TGG | + | 51443:181145-181164 | MS.gene020409:CDS | 65.0% |
| !! | GGTGGTGGTGGTAGTGGAGG+AGG | + | 51443:181151-181170 | MS.gene020409:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGTAGTGG+AGG | + | 51443:181148-181167 | MS.gene020409:CDS | 65.0% |
| !! | TGGTGGTGGAAACCAGGCGG+AGG | - | 51443:181019-181038 | None:intergenic | 65.0% |
| CCACCACCACCGCTGCTGCT+TGG | - | 51443:181139-181158 | None:intergenic | 70.0% | |
| GCAGCGTTACCTCCTCCGCC+TGG | + | 51443:181004-181023 | MS.gene020409:CDS | 70.0% | |
| GGGGTTCCCTGTTACGCCGG+TGG | + | 51443:180619-180638 | MS.gene020409:CDS | 70.0% | |
| ! | CCAAGCAGCAGCGGTGGTGG+TGG | + | 51443:181136-181155 | MS.gene020409:CDS | 70.0% |
| !! | AGCAGCAGCGGTGGTGGTGG+TGG | + | 51443:181139-181158 | MS.gene020409:CDS | 70.0% |
| ACAGCCACCGCCACAGGCGG+TGG | + | 51443:180722-180741 | MS.gene020409:CDS | 75.0% | |
| ACCGCCTGTGGCGGTGGCTG+TGG | - | 51443:180723-180742 | None:intergenic | 75.0% | |
| GACACCACCGCCTGTGGCGG+TGG | - | 51443:180729-180748 | None:intergenic | 75.0% | |
| GCAGCCACAGCCACCGCCAC+AGG | + | 51443:180716-180735 | MS.gene020409:CDS | 75.0% | |
| GCCACAGCCACCGCCACAGG+CGG | + | 51443:180719-180738 | MS.gene020409:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51443 | gene | 180617 | 181378 | 180617 | ID=MS.gene020409 |
| 51443 | mRNA | 180617 | 181378 | 180617 | ID=MS.gene020409.t1;Parent=MS.gene020409 |
| 51443 | exon | 180617 | 181378 | 180617 | ID=MS.gene020409.t1.exon1;Parent=MS.gene020409.t1 |
| 51443 | CDS | 180617 | 181378 | 180617 | ID=cds.MS.gene020409.t1;Parent=MS.gene020409.t1 |
| Gene Sequence |
| Protein sequence |
