Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020449.t1 | XP_003602887.2 | 95.8 | 359 | 14 | 1 | 1 | 358 | 1 | 359 | 9.50E-167 | 596.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020449.t1 | Q10F25 | 49.3 | 229 | 101 | 5 | 68 | 288 | 80 | 301 | 8.5e-47 | 189.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020449.t1 | G7J4K2 | 95.8 | 359 | 14 | 1 | 1 | 358 | 1 | 359 | 6.9e-167 | 596.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020449.t1 | TF | C2C2-GATA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020449.t1 | MTR_3g100040 | 96.657 | 359 | 11 | 1 | 1 | 358 | 1 | 359 | 0.0 | 709 |
| MS.gene020449.t1 | MTR_3g100040 | 96.078 | 255 | 10 | 0 | 104 | 358 | 1 | 255 | 0.0 | 508 |
| MS.gene020449.t1 | MTR_4g093730 | 44.860 | 321 | 131 | 8 | 54 | 358 | 29 | 319 | 1.92e-77 | 241 |
| MS.gene020449.t1 | MTR_5g066510 | 48.000 | 250 | 115 | 7 | 12 | 252 | 14 | 257 | 4.27e-62 | 200 |
| MS.gene020449.t1 | MTR_3g100050 | 52.821 | 195 | 78 | 5 | 68 | 258 | 71 | 255 | 1.19e-57 | 189 |
| MS.gene020449.t1 | MTR_3g060200 | 57.534 | 146 | 57 | 3 | 162 | 304 | 10 | 153 | 2.17e-45 | 158 |
| MS.gene020449.t1 | MTR_3g060200 | 53.614 | 166 | 55 | 3 | 162 | 306 | 10 | 174 | 4.04e-44 | 156 |
| MS.gene020449.t1 | MTR_3g060200 | 53.614 | 166 | 55 | 3 | 162 | 306 | 10 | 174 | 5.22e-44 | 157 |
| MS.gene020449.t1 | MTR_3g060200 | 53.614 | 166 | 55 | 3 | 162 | 306 | 10 | 174 | 3.94e-43 | 157 |
| MS.gene020449.t1 | MTR_5g066510 | 43.655 | 197 | 98 | 6 | 4 | 194 | 10 | 199 | 2.01e-34 | 125 |
| MS.gene020449.t1 | MTR_4g008090 | 50.000 | 74 | 37 | 0 | 86 | 159 | 11 | 84 | 1.88e-16 | 75.1 |
| MS.gene020449.t1 | MTR_4g008070 | 46.914 | 81 | 34 | 3 | 162 | 235 | 1 | 79 | 1.04e-14 | 69.7 |
| MS.gene020449.t1 | MTR_3g037390 | 57.143 | 49 | 21 | 0 | 122 | 170 | 386 | 434 | 7.39e-12 | 66.6 |
| MS.gene020449.t1 | MTR_3g037390 | 57.143 | 49 | 21 | 0 | 122 | 170 | 490 | 538 | 1.13e-11 | 66.2 |
| MS.gene020449.t1 | MTR_4g108880 | 57.143 | 49 | 21 | 0 | 122 | 170 | 497 | 545 | 4.63e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020449.t1 | AT3G21175 | 58.470 | 183 | 67 | 4 | 68 | 243 | 79 | 259 | 8.86e-62 | 200 |
| MS.gene020449.t1 | AT3G21175 | 56.831 | 183 | 72 | 3 | 68 | 243 | 79 | 261 | 9.92e-60 | 194 |
| MS.gene020449.t1 | AT1G51600 | 52.736 | 201 | 76 | 5 | 68 | 253 | 83 | 279 | 1.20e-58 | 192 |
| MS.gene020449.t1 | AT1G51600 | 52.736 | 201 | 76 | 5 | 68 | 253 | 83 | 279 | 1.20e-58 | 192 |
| MS.gene020449.t1 | AT4G24470 | 51.412 | 177 | 82 | 3 | 68 | 243 | 83 | 256 | 9.03e-51 | 172 |
| MS.gene020449.t1 | AT4G24470 | 51.412 | 177 | 82 | 3 | 68 | 243 | 83 | 256 | 9.03e-51 | 172 |
| MS.gene020449.t1 | AT4G24470 | 51.412 | 177 | 82 | 3 | 68 | 243 | 83 | 256 | 1.21e-50 | 172 |
| MS.gene020449.t1 | AT3G21175 | 51.449 | 138 | 60 | 3 | 68 | 198 | 79 | 216 | 1.86e-32 | 121 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAAAACTCACAGAGATTC+AGG | 0.223095 | 3.3:+82468122 | None:intergenic |
| CATGCATTGCACAAAGTTCT+TGG | 0.241152 | 3.3:+82467950 | None:intergenic |
| AGTTCAGCTGTGGGCATGCC+TGG | 0.281391 | 3.3:+82470806 | None:intergenic |
| GCTATTCTTCGTGAAAGATT+TGG | 0.306955 | 3.3:+82470646 | None:intergenic |
| GCAGCTGCAGTTGCAGTTGC+AGG | 0.307759 | 3.3:+82473052 | None:intergenic |
| TGCCATTTGATCAGAGTAAC+AGG | 0.315044 | 3.3:-82470784 | MS.gene020449:CDS |
| GATACAGATATTGACATTCC+TGG | 0.340740 | 3.3:-82465677 | MS.gene020449:CDS |
| GCTGGTCCGCGACGCATTGC+AGG | 0.357972 | 3.3:+82467977 | None:intergenic |
| ATTGGTAATTTGTCATTCTT+CGG | 0.372377 | 3.3:-82466009 | MS.gene020449:CDS |
| TCCTGCAATCTCTAGGGCTT+GGG | 0.375005 | 3.3:-82470685 | MS.gene020449:intron |
| ACCAGACGACTTTGCTCAAC+AGG | 0.377857 | 3.3:+82467265 | None:intergenic |
| TGATGATACTATGGATGAAT+TGG | 0.387456 | 3.3:-82473122 | MS.gene020449:CDS |
| ACCTTCTAATCAAAGTTTCC+AGG | 0.390784 | 3.3:+82465659 | None:intergenic |
| TGCTAGGATGCATCGGGAAA+AGG | 0.402415 | 3.3:-82468228 | MS.gene020449:intron |
| TCAATAGCTGGAATTGTTGC+AGG | 0.402850 | 3.3:+82473181 | None:intergenic |
| ACTCACAGAGATTCAGGATT+AGG | 0.406041 | 3.3:+82468128 | None:intergenic |
| ACAGTTGGCTTGACATCAAC+AGG | 0.416226 | 3.3:+82466370 | None:intergenic |
| GCTAGGATGCATCGGGAAAA+GGG | 0.419663 | 3.3:-82468227 | MS.gene020449:intron |
| ACCTTCTGATGTGTGACATC+GGG | 0.425377 | 3.3:+82472971 | None:intergenic |
| GGAACCAGGGCTTTGCTTCA+AGG | 0.426853 | 3.3:+82468193 | None:intergenic |
| TGATGATGGTGATGATACTA+TGG | 0.427625 | 3.3:-82473131 | MS.gene020449:CDS |
| CACAAAGTTCTTGGTCCAGC+TGG | 0.446059 | 3.3:+82467959 | None:intergenic |
| GCAATGCATGCGGCCTCATG+TGG | 0.449902 | 3.3:-82467937 | MS.gene020449:CDS |
| CTCTAGTTGGAATTCAGCAC+AGG | 0.449996 | 3.3:-82468171 | MS.gene020449:CDS |
| GTCAAGCCAACTGTTCTTGA+AGG | 0.454609 | 3.3:-82466361 | MS.gene020449:CDS |
| ATTTCTTGCTAGGATGCATC+GGG | 0.457873 | 3.3:-82468234 | MS.gene020449:intron |
| CTTTCTAATCCTTCAGATGC+AGG | 0.461274 | 3.3:-82465716 | MS.gene020449:CDS |
| GCAATGCGTCGCGGACCAGC+TGG | 0.462401 | 3.3:-82467974 | MS.gene020449:CDS |
| AGCAAAATGACCAACCTTGT+TGG | 0.463402 | 3.3:+82467913 | None:intergenic |
| GGAGGAAGTGGTGCGCAGCC+AGG | 0.464266 | 3.3:-82470824 | MS.gene020449:CDS |
| TCCTGTTGAGCAAAGTCGTC+TGG | 0.466212 | 3.3:-82467266 | MS.gene020449:intron |
| ACCTTCCGCACCAACAGTCT+TGG | 0.467444 | 3.3:+82466207 | None:intergenic |
| ACCCTGTTACTCTGATCAAA+TGG | 0.485919 | 3.3:+82470782 | None:intergenic |
| GAAGAATAGCCTCCTTGGTC+AGG | 0.487750 | 3.3:-82470633 | MS.gene020449:CDS |
| TCCAAGACTGTTGGTGCGGA+AGG | 0.498699 | 3.3:-82466208 | MS.gene020449:CDS |
| AAAGCCCTGGTTCCTCTAGT+TGG | 0.498939 | 3.3:-82468184 | MS.gene020449:CDS |
| TTCACGAAGAATAGCCTCCT+TGG | 0.502425 | 3.3:-82470638 | MS.gene020449:CDS |
| CGGCCTCATGTGGGCCAACA+AGG | 0.505964 | 3.3:-82467927 | MS.gene020449:intron |
| GAACCAGGGCTTTGCTTCAA+GGG | 0.507428 | 3.3:+82468194 | None:intergenic |
| TATTTCTTGCTAGGATGCAT+CGG | 0.512222 | 3.3:-82468235 | MS.gene020449:intron |
| TTCGGAAAATGATGATGAGC+AGG | 0.513065 | 3.3:-82465991 | MS.gene020449:intron |
| TTAGACATTCCTGCATCTGA+AGG | 0.515486 | 3.3:+82465707 | None:intergenic |
| GCTGCAGCTGCAACACGTAT+TGG | 0.518862 | 3.3:-82473034 | MS.gene020449:CDS |
| AATTCCCCTTCAAGAACAGT+TGG | 0.521168 | 3.3:+82466355 | None:intergenic |
| TGGAATTCAGCACAGGGTGC+TGG | 0.521463 | 3.3:-82468164 | MS.gene020449:CDS |
| GGTAATTCAGTGAACGTTGA+AGG | 0.522137 | 3.3:-82473085 | MS.gene020449:CDS |
| TCGTCGTCGTCATCAATAGC+TGG | 0.535781 | 3.3:+82473169 | None:intergenic |
| ATCAAATGGCAGTTCAGCTG+TGG | 0.540344 | 3.3:+82470796 | None:intergenic |
| TACCTTCTGATGTGTGACAT+CGG | 0.541157 | 3.3:+82472970 | None:intergenic |
| GAAGAACCTTCCAAGACTGT+TGG | 0.545293 | 3.3:-82466217 | MS.gene020449:CDS |
| GACACTCAGAGATCTCAGTA+AGG | 0.560391 | 3.3:-82467302 | MS.gene020449:CDS |
| GCACAGGGTGCTGGTCAAGA+TGG | 0.560830 | 3.3:-82468155 | MS.gene020449:CDS |
| GCACCCTTGAAGCAAAGCCC+TGG | 0.567134 | 3.3:-82468197 | MS.gene020449:CDS |
| ACTACAACTACCTCATCACC+AGG | 0.568839 | 3.3:+82466163 | None:intergenic |
| TCAAGCCAACTGTTCTTGAA+GGG | 0.572288 | 3.3:-82466360 | MS.gene020449:CDS |
| TAGATACTCCGTGAGAAAAG+AGG | 0.572399 | 3.3:-82470569 | MS.gene020449:CDS |
| CCCCGATGTCACACATCAGA+AGG | 0.572507 | 3.3:-82472972 | MS.gene020449:intron |
| CTCAGAGATCTCAGTAAGGG+TGG | 0.581454 | 3.3:-82467298 | MS.gene020449:CDS |
| TGCAATCTCTAGGGCTTGGG+CGG | 0.583296 | 3.3:-82470682 | MS.gene020449:intron |
| GAATTGGATGAAGAAACAAA+TGG | 0.583383 | 3.3:-82473106 | MS.gene020449:CDS |
| GCAGGATCCTCAGATGGAAG+AGG | 0.588835 | 3.3:+82473199 | None:intergenic |
| CAACCTTGTTGGCCCACATG+AGG | 0.589336 | 3.3:+82467924 | None:intergenic |
| TGCATCAAACCCTGGTGATG+AGG | 0.590145 | 3.3:-82466173 | MS.gene020449:intron |
| GAATTCCAACTAGAGGAACC+AGG | 0.591328 | 3.3:+82468179 | None:intergenic |
| CAATGCATGCGGCCTCATGT+GGG | 0.602828 | 3.3:-82467936 | MS.gene020449:CDS |
| TCAAATGGCAGTTCAGCTGT+GGG | 0.609776 | 3.3:+82470797 | None:intergenic |
| ATTGTTGCAGGATCCTCAGA+TGG | 0.609966 | 3.3:+82473193 | None:intergenic |
| TGATGACGACGACGACGATG+AGG | 0.610620 | 3.3:-82473161 | MS.gene020449:CDS |
| ATGTCAATATCTGTATCTGA+AGG | 0.610986 | 3.3:+82465683 | None:intergenic |
| TCCGCACCAACAGTCTTGGA+AGG | 0.612491 | 3.3:+82466211 | None:intergenic |
| GGAACAACCTCTTCCATCTG+AGG | 0.612918 | 3.3:-82473206 | MS.gene020449:CDS |
| GAAGAATGACAAATTACCAA+TGG | 0.616321 | 3.3:+82466011 | None:intergenic |
| GGATTAGAAAGCTCGACCAC+GGG | 0.617309 | 3.3:+82465728 | None:intergenic |
| CAAGCCAACTGTTCTTGAAG+GGG | 0.619999 | 3.3:-82466359 | MS.gene020449:CDS |
| ACCTTCCAAGACTGTTGGTG+CGG | 0.622461 | 3.3:-82466212 | MS.gene020449:CDS |
| CTAGGATGCATCGGGAAAAG+GGG | 0.623092 | 3.3:-82468226 | MS.gene020449:intron |
| TGAGAAAAGAGGTGGCAGAA+AGG | 0.629475 | 3.3:-82470558 | MS.gene020449:intron |
| GATGAGGACTATACTGATGA+TGG | 0.633851 | 3.3:-82473145 | MS.gene020449:CDS |
| AGACGTAGATGTCAACATTG+TGG | 0.641093 | 3.3:-82468019 | MS.gene020449:CDS |
| AGGATTAGAAAGCTCGACCA+CGG | 0.642905 | 3.3:+82465727 | None:intergenic |
| AGAACTTTGTGCAATGCATG+CGG | 0.654215 | 3.3:-82467947 | MS.gene020449:CDS |
| CTGTGCTGAATTCCAACTAG+AGG | 0.664420 | 3.3:+82468172 | None:intergenic |
| AATACTCCTGCAATGCGTCG+CGG | 0.665782 | 3.3:-82467983 | MS.gene020449:CDS |
| ACACTCAGAGATCTCAGTAA+GGG | 0.666946 | 3.3:-82467301 | MS.gene020449:CDS |
| TCTAGTTGGAATTCAGCACA+GGG | 0.669194 | 3.3:-82468170 | MS.gene020449:CDS |
| ATACTCCGTGAGAAAAGAGG+TGG | 0.671679 | 3.3:-82470566 | MS.gene020449:CDS |
| AATGATCATGCATCAAACCC+TGG | 0.673623 | 3.3:-82466181 | MS.gene020449:CDS |
| AATTCCAACTAGAGGAACCA+GGG | 0.673882 | 3.3:+82468180 | None:intergenic |
| CCTTCTGATGTGTGACATCG+GGG | 0.685593 | 3.3:+82472972 | None:intergenic |
| ATATGACCTACAGGAACCCG+TGG | 0.688581 | 3.3:-82465744 | MS.gene020449:intron |
| GTTGGCTTGACATCAACAGG+AGG | 0.690482 | 3.3:+82466373 | None:intergenic |
| TTGGCTTGACATCAACAGGA+GGG | 0.707354 | 3.3:+82466374 | None:intergenic |
| GCCATTTGATCAGAGTAACA+GGG | 0.709466 | 3.3:-82470783 | MS.gene020449:intron |
| CTACAACTACCTCATCACCA+GGG | 0.715371 | 3.3:+82466164 | None:intergenic |
| TTCACGGTACCTGACCAAGG+AGG | 0.733564 | 3.3:+82470624 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 82465664 | 82473233 | 82465664 | ID=MS.gene020449 |
| chr3.3 | mRNA | 82465664 | 82473233 | 82465664 | ID=MS.gene020449.t1;Parent=MS.gene020449 |
| chr3.3 | exon | 82472973 | 82473233 | 82472973 | ID=MS.gene020449.t1.exon1;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82472973 | 82473233 | 82472973 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82470784 | 82470870 | 82470784 | ID=MS.gene020449.t1.exon2;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82470784 | 82470870 | 82470784 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82470559 | 82470692 | 82470559 | ID=MS.gene020449.t1.exon3;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82470559 | 82470692 | 82470559 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82468134 | 82468244 | 82468134 | ID=MS.gene020449.t1.exon4;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82468134 | 82468244 | 82468134 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82467928 | 82468039 | 82467928 | ID=MS.gene020449.t1.exon5;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82467928 | 82468039 | 82467928 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82467267 | 82467326 | 82467267 | ID=MS.gene020449.t1.exon6;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82467267 | 82467326 | 82467267 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82466333 | 82466398 | 82466333 | ID=MS.gene020449.t1.exon7;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82466333 | 82466398 | 82466333 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82466174 | 82466248 | 82466174 | ID=MS.gene020449.t1.exon8;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82466174 | 82466248 | 82466174 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82465992 | 82466072 | 82465992 | ID=MS.gene020449.t1.exon9;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82465992 | 82466072 | 82465992 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| chr3.3 | exon | 82465664 | 82465753 | 82465664 | ID=MS.gene020449.t1.exon10;Parent=MS.gene020449.t1 |
| chr3.3 | CDS | 82465664 | 82465753 | 82465664 | ID=cds.MS.gene020449.t1;Parent=MS.gene020449.t1 |
| Gene Sequence |
| Protein sequence |
