AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020513

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020513.t1 MTR_8g105200 97.526 485 9 2 1 485 1 482 0.0 959
MS.gene020513.t1 MTR_5g010630 92.233 103 5 2 200 302 5 104 1.87e-60 193
MS.gene020513.t1 MTR_2g067520 88.406 69 8 0 200 268 5 73 1.83e-37 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020513.t1 AT1G24610 65.895 475 155 4 11 482 4 474 0.0 629
MS.gene020513.t1 AT1G14030 24.558 452 287 18 23 461 29 439 9.28e-14 73.9
MS.gene020513.t1 AT4G20130 22.603 438 269 15 55 468 83 474 6.58e-12 68.2

Find 113 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTCATCATCCATTAATT+TGG 0.191527 4.3:-1427998 None:intergenic
CAAATATGATGGTGCTCTTT+TGG 0.205619 4.3:+1426720 MS.gene020513:CDS
CTCGATTCCTCAAATGGTTT+AGG 0.230593 4.3:+1425059 MS.gene020513:CDS
ACACTTTCCCACCAAATTAA+TGG 0.235094 4.3:+1427987 MS.gene020513:CDS
CTATAATTACAGACTTCTTT+TGG 0.248268 4.3:-1428074 None:intergenic
CATCCATAGCAAAGTAATAA+AGG 0.272419 4.3:-1426625 None:intergenic
TCGATTCCTCAAATGGTTTA+GGG 0.287954 4.3:+1425060 MS.gene020513:CDS
TCCTGAACAATTCTAGCATT+TGG 0.291593 4.3:-1426390 None:intergenic
TTATTGAAGCAGGGTGCTTC+AGG 0.306570 4.3:+1428021 MS.gene020513:CDS
CCACCAAACGGATGCTTATC+TGG 0.313373 4.3:-1426216 None:intergenic
CTCAAATGGTTTAGGGTTAG+TGG 0.331608 4.3:+1425067 MS.gene020513:CDS
TCTTCCCCAGGAAAGAAAAT+AGG 0.342405 4.3:-1425490 None:intergenic
TGAATTGTGGGCAATGAAAC+TGG 0.345575 4.3:+1425384 MS.gene020513:CDS
GAGATGAAGCATCTACTTCT+TGG 0.347456 4.3:-1426239 None:intergenic
GTGGAAGATACCTTGAGATC+TGG 0.349462 4.3:-1426844 None:intergenic
TCCAAATGCTAGAATTGTTC+AGG 0.369501 4.3:+1426389 MS.gene020513:CDS
CAATTAACCTCCCATGTTCC+CGG 0.370584 4.3:+1425191 MS.gene020513:CDS
GGAGCAGGTGTAGAGAAATT+TGG 0.377711 4.3:-1426775 None:intergenic
GAAGTAGATGCTTCATCTCT+TGG 0.384991 4.3:+1426243 MS.gene020513:CDS
CATGGTAACAAGCAATCAAA+TGG 0.388478 4.3:+1426306 MS.gene020513:CDS
CACCCACATCTTTGTGCTAC+CGG 0.389753 4.3:-1426421 None:intergenic
AGTACTCCTTGCAAGTGATA+TGG 0.393467 4.3:+1427843 MS.gene020513:CDS
CTTCATGAAAGGGCAAAACT+TGG 0.399181 4.3:+1425418 MS.gene020513:CDS
GCTCAAAATCAAGAAGAAAT+CGG 0.402032 4.3:-1426161 None:intergenic
TGGAAGATACCTTGAGATCT+GGG 0.409477 4.3:-1426843 None:intergenic
GCAATTAGGTCAGTACCAAT+TGG 0.411464 4.3:-1425104 None:intergenic
CACTAACCCTAAACCATTTG+AGG 0.419235 4.3:-1425066 None:intergenic
ATCCACACGATCTCATCAAA+TGG 0.420118 4.3:+1424988 MS.gene020513:CDS
GACCCTTTATTACTTTGCTA+TGG 0.431078 4.3:+1426622 MS.gene020513:CDS
CTGGTTAATATGGTTATAGT+TGG 0.431597 4.3:+1424855 None:intergenic
AATAATAGAAGGTCGTTTGT+TGG 0.431982 4.3:+1427810 MS.gene020513:CDS
GCACAACTCGATTCCTCAAA+TGG 0.432361 4.3:+1425053 MS.gene020513:CDS
TTAGATGCTGAGGCTCCTCT+TGG 0.432705 4.3:+1427904 MS.gene020513:CDS
ATCCATAGCAAAGTAATAAA+GGG 0.432856 4.3:-1426624 None:intergenic
TGATTACACATGTCAATTAG+AGG 0.440828 4.3:-1426357 None:intergenic
GATGAAGATGCGCATGTTAG+TGG 0.442848 4.3:-1424933 None:intergenic
AAGTGTACCTGGTGAAGAAT+AGG 0.446363 4.3:-1425535 None:intergenic
TTATAGTTGGTGGTGGTAGT+TGG 0.452028 4.3:+1424868 MS.gene020513:CDS
TTGAGCTCGATACAGTCATA+AGG 0.453734 4.3:-1426700 None:intergenic
TAGGAAGAGTGTAAGTTTGT+GGG 0.460771 4.3:-1425471 None:intergenic
GTTAATATGGTTATAGTTGG+TGG 0.472157 4.3:+1424858 None:intergenic
GATGCTGCGAGCATGGCAGC+TGG 0.474563 4.3:+1426742 MS.gene020513:CDS
TGAGCTCGATACAGTCATAA+GGG 0.476129 4.3:-1426699 None:intergenic
TGATGAATCTTTATTGAAGC+AGG 0.476763 4.3:+1428011 MS.gene020513:CDS
TTCATCATCCATTAATTTGG+TGG 0.478727 4.3:-1427995 None:intergenic
TTCATGTAACAGGTGAGCTT+AGG 0.480929 4.3:+1427778 MS.gene020513:intron
ATGTAACAGGTGAGCTTAGG+TGG 0.482998 4.3:+1427781 MS.gene020513:intron
TGTGTTGAGGAAGAGCAATT+AGG 0.483022 4.3:-1425118 None:intergenic
ATAGGAAGAGTGTAAGTTTG+TGG 0.491387 4.3:-1425472 None:intergenic
CAATTAGGTCAGTACCAATT+GGG 0.491707 4.3:-1425103 None:intergenic
TTTGAAGCTTCTTCATGAAA+GGG 0.494538 4.3:+1425408 MS.gene020513:CDS
GTTTGAAGCTTCTTCATGAA+AGG 0.494844 4.3:+1425407 MS.gene020513:CDS
CTCTACACCTGCTCCATGGC+AGG 0.495127 4.3:+1426783 MS.gene020513:CDS
TGGTTTAGGGTTAGTGGCTA+AGG 0.496899 4.3:+1425073 MS.gene020513:CDS
ACACCAGATAAGCATCCGTT+TGG 0.499859 4.3:+1426213 MS.gene020513:CDS
GCTAAGGAAGATATCCCAAT+TGG 0.505887 4.3:+1425089 MS.gene020513:CDS
TGTGGGAGATTACTGATGTA+TGG 0.507516 4.3:-1425454 None:intergenic
GCATTTGGCAGATGAATTGT+GGG 0.511625 4.3:+1425372 MS.gene020513:intron
CCAGATAAGCATCCGTTTGG+TGG 0.512174 4.3:+1426216 MS.gene020513:CDS
TCAAGAGCATTCAAGTTACA+TGG 0.514609 4.3:+1426288 MS.gene020513:CDS
GGTAAAGCTTCTTTCATCAA+AGG 0.514902 4.3:+1428122 MS.gene020513:CDS
GGTGGGCAAGAAATAATAGA+AGG 0.517366 4.3:+1427799 MS.gene020513:CDS
TAGATGCTTCATCTCTTGGA+TGG 0.521095 4.3:+1426247 MS.gene020513:CDS
TCATCATCCATTAATTTGGT+GGG 0.525608 4.3:-1427994 None:intergenic
ATAAGTTCCTGCCATGGAGC+AGG 0.528390 4.3:-1426790 None:intergenic
GAAGCTTTACCTTCCTTGAG+AGG 0.528724 4.3:-1428110 None:intergenic
CTATCTCATCTAAATCTAGC+TGG 0.528818 4.3:+1426814 MS.gene020513:CDS
AATGACACACAGAGCAATCA+AGG 0.529045 4.3:-1427956 None:intergenic
AATATGGTTATAGTTGGTGG+TGG 0.531556 4.3:+1424861 None:intergenic
TCAATTAGAGGGAGCATCAT+GGG 0.532994 4.3:-1426345 None:intergenic
TCCACACGATCTCATCAAAT+GGG 0.536822 4.3:+1424989 MS.gene020513:CDS
TGGTGGTGGTAGTTGGAAAA+CGG 0.543989 4.3:+1424875 MS.gene020513:CDS
GTCAATTAGAGGGAGCATCA+TGG 0.544285 4.3:-1426346 None:intergenic
ATGGTAACAAGCAATCAAAT+GGG 0.547291 4.3:+1426307 MS.gene020513:CDS
TCACAAATCCATAATCAAGA+AGG 0.549483 4.3:-1426666 None:intergenic
TCTACTTCTTGGCCACCAAA+CGG 0.549674 4.3:-1426228 None:intergenic
ATCAAATGGGTAACAAGAGA+AGG 0.550397 4.3:+1425002 MS.gene020513:CDS
TGTGGCTGAGAAAGCAATCA+AGG 0.555448 4.3:+1426594 MS.gene020513:CDS
AGCATTTGGCAGATGAATTG+TGG 0.556575 4.3:+1425371 MS.gene020513:intron
CTATGCTCCTATTCTTCACC+AGG 0.562752 4.3:+1425528 MS.gene020513:CDS
TGGAGAAACCCCAGATCTCA+AGG 0.564415 4.3:+1426834 MS.gene020513:CDS
AAGCCGGTAGCACAAAGATG+TGG 0.568257 4.3:+1426418 MS.gene020513:CDS
GCTTCTTTCATCAAAGGAGA+CGG 0.577964 4.3:+1428128 MS.gene020513:CDS
AAGGAGACGGTCACTACTGA+AGG 0.581558 4.3:+1428141 MS.gene020513:CDS
GATGAATCTTTATTGAAGCA+GGG 0.583312 4.3:+1428012 MS.gene020513:CDS
CACTTGCCATATCACTTGCA+AGG 0.586602 4.3:-1427849 None:intergenic
CATGGGAGGTTAATTGAAGG+AGG 0.592569 4.3:-1425184 None:intergenic
AAATTCTTGATGTCTTCCCC+AGG 0.592666 4.3:-1425502 None:intergenic
TTGATGAGATCGTGTGGATG+AGG 0.593115 4.3:-1424984 None:intergenic
AAAGTCAAGCATGCATTAGT+CGG 0.594145 4.3:+1426186 MS.gene020513:CDS
GCTCTGTGTGTCATTGCACT+AGG 0.596790 4.3:+1427964 MS.gene020513:CDS
GAACATGGGAGGTTAATTGA+AGG 0.597020 4.3:-1425187 None:intergenic
GATTACACATGTCAATTAGA+GGG 0.597579 4.3:-1426356 None:intergenic
ACTTAAGTCTTTAGATGCTG+AGG 0.601367 4.3:+1427894 MS.gene020513:CDS
AAGTGCAGAGGAATGTGTTG+AGG 0.602415 4.3:-1425131 None:intergenic
CTAAAGACTTAAGTGTGTTG+AGG 0.608231 4.3:-1427885 None:intergenic
AAATGGGTAACAAGAGAAGG+TGG 0.609234 4.3:+1425005 MS.gene020513:CDS
AGATGCTTCATCTCTTGGAT+GGG 0.610544 4.3:+1426248 MS.gene020513:CDS
ATTTCTCTACACCTGCTCCA+TGG 0.612721 4.3:+1426779 MS.gene020513:CDS
TAGCACAAAGATGTGGGTGA+AGG 0.614670 4.3:+1426425 MS.gene020513:CDS
AGCCGGTAGCACAAAGATGT+GGG 0.619848 4.3:+1426419 MS.gene020513:CDS
GATAGAATAAGTTCCTGCCA+TGG 0.621183 4.3:-1426796 None:intergenic
ATTGTTCAGGAACAAGAAGC+CGG 0.621234 4.3:+1426402 MS.gene020513:CDS
TGTAACAGGTGAGCTTAGGT+GGG 0.630878 4.3:+1427782 MS.gene020513:intron
TCATCATCGTTGAAGTGCAG+AGG 0.633506 4.3:-1425143 None:intergenic
GGAAGATACCTTGAGATCTG+GGG 0.633639 4.3:-1426842 None:intergenic
GAATTGTGGGCAATGAAACT+GGG 0.634097 4.3:+1425385 MS.gene020513:CDS
TGTATCGAGCTCAAATATGA+TGG 0.639565 4.3:+1426709 MS.gene020513:CDS
AGGTCATTTGCAACACCAAG+AGG 0.646673 4.3:-1427919 None:intergenic
TTACTTACCGGGAACATGGG+AGG 0.654333 4.3:-1425198 None:intergenic
ACCCATTTGATGAGATCGTG+TGG 0.664082 4.3:-1424990 None:intergenic
TCAAGGTACGAAATACAGCA+AGG 0.687972 4.3:-1427939 None:intergenic
GTTGTGCTATCTTCACAGCA+CGG 0.695139 4.3:-1425037 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTTATTAACCATTCAA+TGG - chr4.3:1427047-1427066 None:intergenic 15.0%
!! TTAAAAATGAAAACTACTAT+TGG + chr4.3:1426899-1426918 MS.gene020513:intron 15.0%
!!! AATGTTTTTATAGCAAATAT+TGG + chr4.3:1427587-1427606 MS.gene020513:intron 15.0%
!!! TTCAAATATACATGATTTTA+AGG - chr4.3:1427212-1427231 None:intergenic 15.0%
!! AAAATTAGTATAATGCTTTG+TGG + chr4.3:1425603-1425622 MS.gene020513:intron 20.0%
!! AAAGTTCATAGTTATTCTAT+GGG - chr4.3:1427561-1427580 None:intergenic 20.0%
!! AACATAATGCGTAAAAATAA+TGG - chr4.3:1427515-1427534 None:intergenic 20.0%
!! AGAAATTCAATATTTACCTT+AGG + chr4.3:1426570-1426589 MS.gene020513:intron 20.0%
!! ATAACTAAATCGTGATAATT+AGG - chr4.3:1425966-1425985 None:intergenic 20.0%
!! TAAATCAAATTATGACCTTA+TGG - chr4.3:1427753-1427772 None:intergenic 20.0%
!! TTTAGATCGTTAAGTATATA+TGG - chr4.3:1427367-1427386 None:intergenic 20.0%
!!! ATTTACTTTTTTCATGTAAC+AGG + chr4.3:1427768-1427787 MS.gene020513:intron 20.0%
!!! GTCATTATACATAATTTTTC+AGG - chr4.3:1427295-1427314 None:intergenic 20.0%
! AAATTGCTCATTTCAAAAGT+GGG - chr4.3:1425997-1426016 None:intergenic 25.0%
! AGATTCATCATCCATTAATT+TGG - chr4.3:1428001-1428020 None:intergenic 25.0%
! ATCCATAGCAAAGTAATAAA+GGG - chr4.3:1426627-1426646 None:intergenic 25.0%
! ATGTATATTTGAATCCAGTT+TGG + chr4.3:1427219-1427238 MS.gene020513:intron 25.0%
! CAGTTTGGATGATTAATATA+TGG + chr4.3:1427234-1427253 MS.gene020513:intron 25.0%
! CATATATTAATCATCCAAAC+TGG - chr4.3:1427236-1427255 None:intergenic 25.0%
! GAAAGTTCATAGTTATTCTA+TGG - chr4.3:1427562-1427581 None:intergenic 25.0%
! GAGAAATGCATATAAGTATA+CGG + chr4.3:1425699-1425718 MS.gene020513:intron 25.0%
! GAGATTAATGACATTCAAAA+TGG - chr4.3:1427122-1427141 None:intergenic 25.0%
! TAAAAACCAGTGGTAAAATT+CGG + chr4.3:1425759-1425778 MS.gene020513:intron 25.0%
!! AATACAGCTATAGAATTTTG+TGG - chr4.3:1426866-1426885 None:intergenic 25.0%
!! ACCAATTGATATGAAAGTTT+TGG - chr4.3:1427713-1427732 None:intergenic 25.0%
!! CCAATTGATATGAAAGTTTT+GGG - chr4.3:1427712-1427731 None:intergenic 25.0%
!! CTATAATTACAGACTTCTTT+TGG - chr4.3:1428077-1428096 None:intergenic 25.0%
!!! AAATTCGGTGTAAATAATGT+TGG + chr4.3:1425774-1425793 MS.gene020513:intron 25.0%
!!! TAATTATTTTGAATGCCTTG+CGG + chr4.3:1426075-1426094 MS.gene020513:intron 25.0%
!!! TGCGTAAAAATAATGGATTA+TGG - chr4.3:1427508-1427527 None:intergenic 25.0%
AATAATAGAAGGTCGTTTGT+TGG + chr4.3:1427810-1427829 MS.gene020513:CDS 30.0%
AATTGCTCATTTCAAAAGTG+GGG - chr4.3:1425996-1426015 None:intergenic 30.0%
ACATTCAAAATGGTCGTTAA+CGG - chr4.3:1427112-1427131 None:intergenic 30.0%
ATATATGTGTTTGTGTGTGT+TGG + chr4.3:1427265-1427284 MS.gene020513:intron 30.0%
ATGGTAACAAGCAATCAAAT+GGG + chr4.3:1426307-1426326 MS.gene020513:CDS 30.0%
ATGTAATGAAAAACCTCTCA+AGG + chr4.3:1428097-1428116 MS.gene020513:CDS 30.0%
ATTAGTATTGTCTCTTTCAC+CGG + chr4.3:1427621-1427640 MS.gene020513:intron 30.0%
CAAATTGCTCATTTCAAAAG+TGG - chr4.3:1425998-1426017 None:intergenic 30.0%
CATCCATAGCAAAGTAATAA+AGG - chr4.3:1426628-1426647 None:intergenic 30.0%
CCCAAAACTTTCATATCAAT+TGG + chr4.3:1427709-1427728 MS.gene020513:intron 30.0%
CTCAGTGTCTATTAATTATG+TGG + chr4.3:1425921-1425940 MS.gene020513:intron 30.0%
GATGAATCTTTATTGAAGCA+GGG + chr4.3:1428012-1428031 MS.gene020513:CDS 30.0%
GATTACACATGTCAATTAGA+GGG - chr4.3:1426359-1426378 None:intergenic 30.0%
GCTCAAAATCAAGAAGAAAT+CGG - chr4.3:1426164-1426183 None:intergenic 30.0%
GTATGTTTGAACACAAATTG+TGG + chr4.3:1427155-1427174 MS.gene020513:intron 30.0%
GTTGAATAAATTGGACAATG+TGG + chr4.3:1425347-1425366 MS.gene020513:intron 30.0%
TCACAAATCCATAATCAAGA+AGG - chr4.3:1426669-1426688 None:intergenic 30.0%
TGAATAAGTTAAGAGGATTC+AGG - chr4.3:1427655-1427674 None:intergenic 30.0%
TGATGAATCTTTATTGAAGC+AGG + chr4.3:1428011-1428030 MS.gene020513:CDS 30.0%
TGATTACACATGTCAATTAG+AGG - chr4.3:1426360-1426379 None:intergenic 30.0%
TTCATAGTTATTCTATGGGA+AGG - chr4.3:1427557-1427576 None:intergenic 30.0%
! GATCTTTTCCTTCTTGATTA+TGG + chr4.3:1426658-1426677 MS.gene020513:CDS 30.0%
! GATTTTTGTGTAAAAACCAG+TGG + chr4.3:1425749-1425768 MS.gene020513:intron 30.0%
! TCAATATTTACCTTAGGTTG+TGG + chr4.3:1426576-1426595 MS.gene020513:intron 30.0%
! TCATCATCCATTAATTTGGT+GGG - chr4.3:1427997-1428016 None:intergenic 30.0%
! TTATCTCCAAGTGTAGTTTT+GGG + chr4.3:1426030-1426049 MS.gene020513:intron 30.0%
! TTCATCATCCATTAATTTGG+TGG - chr4.3:1427998-1428017 None:intergenic 30.0%
! TTTATCTCCAAGTGTAGTTT+TGG + chr4.3:1426029-1426048 MS.gene020513:intron 30.0%
! TTTGAAGCTTCTTCATGAAA+GGG + chr4.3:1425408-1425427 MS.gene020513:CDS 30.0%
!! TTTCTTTCTAGAGTCTTGTT+TGG - chr4.3:1425306-1425325 None:intergenic 30.0%
!!! CACTTTTGAAATGAGCAATT+TGG + chr4.3:1425996-1426015 MS.gene020513:intron 30.0%
AAAGTCAAGCATGCATTAGT+CGG + chr4.3:1426186-1426205 MS.gene020513:CDS 35.0%
AAATTCATGAATCACGAGAC+AGG + chr4.3:1426125-1426144 MS.gene020513:intron 35.0%
AACAGTAAGTAAAGTGTACC+TGG - chr4.3:1425549-1425568 None:intergenic 35.0%
AATGAAAAACCTCTCAAGGA+AGG + chr4.3:1428101-1428120 MS.gene020513:CDS 35.0%
AATTCATGAGCTAATTGAGC+TGG - chr4.3:1425229-1425248 None:intergenic 35.0%
ACACTTGGAGATAAACTATG+CGG - chr4.3:1426024-1426043 None:intergenic 35.0%
ACACTTTCCCACCAAATTAA+TGG + chr4.3:1427987-1428006 MS.gene020513:CDS 35.0%
ACTTAAGTCTTTAGATGCTG+AGG + chr4.3:1427894-1427913 MS.gene020513:CDS 35.0%
AGATAGACAATTGTAGCTTC+TGG - chr4.3:1425903-1425922 None:intergenic 35.0%
ATCAAATGGGTAACAAGAGA+AGG + chr4.3:1425002-1425021 MS.gene020513:CDS 35.0%
ATTGCTCATTTCAAAAGTGG+GGG - chr4.3:1425995-1426014 None:intergenic 35.0%
CAAGGGTTGAATAAGTTAAG+AGG - chr4.3:1427662-1427681 None:intergenic 35.0%
CAATTAGGTCAGTACCAATT+GGG - chr4.3:1425106-1425125 None:intergenic 35.0%
CATGGTAACAAGCAATCAAA+TGG + chr4.3:1426306-1426325 MS.gene020513:CDS 35.0%
CTAAAGACTTAAGTGTGTTG+AGG - chr4.3:1427888-1427907 None:intergenic 35.0%
CTATCTCATCTAAATCTAGC+TGG + chr4.3:1426814-1426833 MS.gene020513:CDS 35.0%
CTCTTAACTTATTCAACCCT+TGG + chr4.3:1427660-1427679 MS.gene020513:intron 35.0%
GACCCTTTATTACTTTGCTA+TGG + chr4.3:1426622-1426641 MS.gene020513:CDS 35.0%
GGTAAAGCTTCTTTCATCAA+AGG + chr4.3:1428122-1428141 MS.gene020513:CDS 35.0%
GGTTAATTACGTCCATTGAA+TGG + chr4.3:1427032-1427051 MS.gene020513:intron 35.0%
GTCGTCGATGTTGAATAAAT+TGG + chr4.3:1425338-1425357 MS.gene020513:intron 35.0%
TCCAAATGCTAGAATTGTTC+AGG + chr4.3:1426389-1426408 MS.gene020513:CDS 35.0%
TCCTGAACAATTCTAGCATT+TGG - chr4.3:1426393-1426412 None:intergenic 35.0%
TCGATTCCTCAAATGGTTTA+GGG + chr4.3:1425060-1425079 MS.gene020513:CDS 35.0%
TCGGTGTAAATAATGTTGGT+AGG + chr4.3:1425778-1425797 MS.gene020513:intron 35.0%
TGTATCGAGCTCAAATATGA+TGG + chr4.3:1426709-1426728 MS.gene020513:CDS 35.0%
TTAGTATTGTCTCTTTCACC+GGG + chr4.3:1427622-1427641 MS.gene020513:intron 35.0%
! AATTTTACTTACCGGGAACA+TGG - chr4.3:1425205-1425224 None:intergenic 35.0%
! ACTCTTCCTATTTTCTTTCC+TGG + chr4.3:1425484-1425503 MS.gene020513:CDS 35.0%
! AGCTGGTAATTTTACTTACC+GGG - chr4.3:1425212-1425231 None:intergenic 35.0%
! ATAGGAAGAGTGTAAGTTTG+TGG - chr4.3:1425475-1425494 None:intergenic 35.0%
! ATTTTACTTACCGGGAACAT+GGG - chr4.3:1425204-1425223 None:intergenic 35.0%
! CAAAACTTGGATCTTTTTGG+TGG + chr4.3:1425431-1425450 MS.gene020513:CDS 35.0%
! CTCTTCCTATTTTCTTTCCT+GGG + chr4.3:1425485-1425504 MS.gene020513:CDS 35.0%
! CTTTTGGATTCTGAACTGAA+TGG - chr4.3:1428061-1428080 None:intergenic 35.0%
! GAGCTGGTAATTTTACTTAC+CGG - chr4.3:1425213-1425232 None:intergenic 35.0%
! GTTTGAAGCTTCTTCATGAA+AGG + chr4.3:1425407-1425426 MS.gene020513:CDS 35.0%
! TAGGAAGAGTGTAAGTTTGT+GGG - chr4.3:1425474-1425493 None:intergenic 35.0%
! TCTTCCTATTTTCTTTCCTG+GGG + chr4.3:1425486-1425505 MS.gene020513:CDS 35.0%
! TGTTTTCCCTCTGTTCATTA+AGG + chr4.3:1427531-1427550 MS.gene020513:intron 35.0%
! TTTACACCGAATTTTACCAC+TGG - chr4.3:1425768-1425787 None:intergenic 35.0%
!! CAAATATGATGGTGCTCTTT+TGG + chr4.3:1426720-1426739 MS.gene020513:CDS 35.0%
!! TATCTCCAAGTGTAGTTTTG+GGG + chr4.3:1426031-1426050 MS.gene020513:intron 35.0%
!! TCAAGAGCATTCAAGTTACA+TGG + chr4.3:1426288-1426307 MS.gene020513:CDS 35.0%
AAATGGGTAACAAGAGAAGG+TGG + chr4.3:1425005-1425024 MS.gene020513:CDS 40.0%
AAATTCTTGATGTCTTCCCC+AGG - chr4.3:1425505-1425524 None:intergenic 40.0%
AAGTGTACCTGGTGAAGAAT+AGG - chr4.3:1425538-1425557 None:intergenic 40.0%
AATGACACACAGAGCAATCA+AGG - chr4.3:1427959-1427978 None:intergenic 40.0%
AGATGCTTCATCTCTTGGAT+GGG + chr4.3:1426248-1426267 MS.gene020513:CDS 40.0%
AGCATTTGGCAGATGAATTG+TGG + chr4.3:1425371-1425390 MS.gene020513:intron 40.0%
AGTACTCCTTGCAAGTGATA+TGG + chr4.3:1427843-1427862 MS.gene020513:CDS 40.0%
ATCCACACGATCTCATCAAA+TGG + chr4.3:1424988-1425007 MS.gene020513:CDS 40.0%
ATGTTAGCACAAGAGCCATA+AGG + chr4.3:1427735-1427754 MS.gene020513:intron 40.0%
ATTGTTCAGGAACAAGAAGC+CGG + chr4.3:1426402-1426421 MS.gene020513:CDS 40.0%
CACTAACCCTAAACCATTTG+AGG - chr4.3:1425069-1425088 None:intergenic 40.0%
CTCGATTCCTCAAATGGTTT+AGG + chr4.3:1425059-1425078 MS.gene020513:CDS 40.0%
CTTCATGAAAGGGCAAAACT+TGG + chr4.3:1425418-1425437 MS.gene020513:CDS 40.0%
GAACATGGGAGGTTAATTGA+AGG - chr4.3:1425190-1425209 None:intergenic 40.0%
GAAGTAGATGCTTCATCTCT+TGG + chr4.3:1426243-1426262 MS.gene020513:CDS 40.0%
GAATTGTGGGCAATGAAACT+GGG + chr4.3:1425385-1425404 MS.gene020513:CDS 40.0%
GATAGAATAAGTTCCTGCCA+TGG - chr4.3:1426799-1426818 None:intergenic 40.0%
GCAATTAGGTCAGTACCAAT+TGG - chr4.3:1425107-1425126 None:intergenic 40.0%
GCATTTGGCAGATGAATTGT+GGG + chr4.3:1425372-1425391 MS.gene020513:intron 40.0%
GCTAAGGAAGATATCCCAAT+TGG + chr4.3:1425089-1425108 MS.gene020513:CDS 40.0%
GCTTACTGTACTTTGCAGAA+AGG + chr4.3:1427446-1427465 MS.gene020513:intron 40.0%
GCTTCTTTCATCAAAGGAGA+CGG + chr4.3:1428128-1428147 MS.gene020513:CDS 40.0%
GGTGGGCAAGAAATAATAGA+AGG + chr4.3:1427799-1427818 MS.gene020513:CDS 40.0%
GTTAGCGCTGTTAAAGTATG+TGG + chr4.3:1427011-1427030 MS.gene020513:intron 40.0%
TAGATGCTTCATCTCTTGGA+TGG + chr4.3:1426247-1426266 MS.gene020513:CDS 40.0%
TAGTATTGTCTCTTTCACCG+GGG + chr4.3:1427623-1427642 MS.gene020513:intron 40.0%
TCAAGGTACGAAATACAGCA+AGG - chr4.3:1427942-1427961 None:intergenic 40.0%
TCCACACGATCTCATCAAAT+GGG + chr4.3:1424989-1425008 MS.gene020513:CDS 40.0%
TCTTCCCCAGGAAAGAAAAT+AGG - chr4.3:1425493-1425512 None:intergenic 40.0%
TGAATTGTGGGCAATGAAAC+TGG + chr4.3:1425384-1425403 MS.gene020513:CDS 40.0%
TGAGCTCGATACAGTCATAA+GGG - chr4.3:1426702-1426721 None:intergenic 40.0%
TGGAAGATACCTTGAGATCT+GGG - chr4.3:1426846-1426865 None:intergenic 40.0%
TGTGGGAGATTACTGATGTA+TGG - chr4.3:1425457-1425476 None:intergenic 40.0%
TGTGTTGAGGAAGAGCAATT+AGG - chr4.3:1425121-1425140 None:intergenic 40.0%
TTCATGTAACAGGTGAGCTT+AGG + chr4.3:1427778-1427797 MS.gene020513:intron 40.0%
TTGAGCTCGATACAGTCATA+AGG - chr4.3:1426703-1426722 None:intergenic 40.0%
TTGGACAATGTGGTAGCATT+TGG + chr4.3:1425357-1425376 MS.gene020513:intron 40.0%
! CTCAAATGGTTTAGGGTTAG+TGG + chr4.3:1425067-1425086 MS.gene020513:CDS 40.0%
! GAGATGAAGCATCTACTTCT+TGG - chr4.3:1426242-1426261 None:intergenic 40.0%
! GGGCAAAACTTGGATCTTTT+TGG + chr4.3:1425428-1425447 MS.gene020513:CDS 40.0%
!! TCAATTAGAGGGAGCATCAT+GGG - chr4.3:1426348-1426367 None:intergenic 40.0%
!! TTATAGTTGGTGGTGGTAGT+TGG + chr4.3:1424868-1424887 MS.gene020513:CDS 40.0%
AAGTGCAGAGGAATGTGTTG+AGG - chr4.3:1425134-1425153 None:intergenic 45.0%
ACAATTGTAGCTTCTGGTGC+TGG - chr4.3:1425897-1425916 None:intergenic 45.0%
ACACCAGATAAGCATCCGTT+TGG + chr4.3:1426213-1426232 MS.gene020513:CDS 45.0%
ACCCATTTGATGAGATCGTG+TGG - chr4.3:1424993-1425012 None:intergenic 45.0%
AGGTCATTTGCAACACCAAG+AGG - chr4.3:1427922-1427941 None:intergenic 45.0%
AGTTGCACATTGCTCTTGTG+AGG - chr4.3:1425824-1425843 None:intergenic 45.0%
ATGTAACAGGTGAGCTTAGG+TGG + chr4.3:1427781-1427800 MS.gene020513:intron 45.0%
ATTTCTCTACACCTGCTCCA+TGG + chr4.3:1426779-1426798 MS.gene020513:CDS 45.0%
CAATTAACCTCCCATGTTCC+CGG + chr4.3:1425191-1425210 MS.gene020513:CDS 45.0%
CACTTGCCATATCACTTGCA+AGG - chr4.3:1427852-1427871 None:intergenic 45.0%
CATGGGAGGTTAATTGAAGG+AGG - chr4.3:1425187-1425206 None:intergenic 45.0%
CTATGCTCCTATTCTTCACC+AGG + chr4.3:1425528-1425547 MS.gene020513:CDS 45.0%
GAAGCTTTACCTTCCTTGAG+AGG - chr4.3:1428113-1428132 None:intergenic 45.0%
GATGAAGATGCGCATGTTAG+TGG - chr4.3:1424936-1424955 None:intergenic 45.0%
GCACAACTCGATTCCTCAAA+TGG + chr4.3:1425053-1425072 MS.gene020513:CDS 45.0%
GGAAGATACCTTGAGATCTG+GGG - chr4.3:1426845-1426864 None:intergenic 45.0%
GGAAGGCCTTAATGAACAGA+GGG - chr4.3:1427540-1427559 None:intergenic 45.0%
GGAGCAGGTGTAGAGAAATT+TGG - chr4.3:1426778-1426797 None:intergenic 45.0%
GTGGAAGATACCTTGAGATC+TGG - chr4.3:1426847-1426866 None:intergenic 45.0%
GTTGTGCTATCTTCACAGCA+CGG - chr4.3:1425040-1425059 None:intergenic 45.0%
TAGCACAAAGATGTGGGTGA+AGG + chr4.3:1426425-1426444 MS.gene020513:CDS 45.0%
TCATCATCGTTGAAGTGCAG+AGG - chr4.3:1425146-1425165 None:intergenic 45.0%
TCTACTTCTTGGCCACCAAA+CGG - chr4.3:1426231-1426250 None:intergenic 45.0%
TGACACCCCAAAACTACACT+TGG - chr4.3:1426039-1426058 None:intergenic 45.0%
TGTAACAGGTGAGCTTAGGT+GGG + chr4.3:1427782-1427801 MS.gene020513:intron 45.0%
TGTGGCTGAGAAAGCAATCA+AGG + chr4.3:1426594-1426613 MS.gene020513:CDS 45.0%
TTGATGAGATCGTGTGGATG+AGG - chr4.3:1424987-1425006 None:intergenic 45.0%
! TGGTTTAGGGTTAGTGGCTA+AGG + chr4.3:1425073-1425092 MS.gene020513:CDS 45.0%
! TTATTGAAGCAGGGTGCTTC+AGG + chr4.3:1428021-1428040 MS.gene020513:CDS 45.0%
!! AGCACAACTGATTTGAGCCA+AGG - chr4.3:1427680-1427699 None:intergenic 45.0%
!! GCACAACTGATTTGAGCCAA+GGG - chr4.3:1427679-1427698 None:intergenic 45.0%
!! GTCAATTAGAGGGAGCATCA+TGG - chr4.3:1426349-1426368 None:intergenic 45.0%
!! TGGTGGTGGTAGTTGGAAAA+CGG + chr4.3:1424875-1424894 MS.gene020513:CDS 45.0%
AAGCCGGTAGCACAAAGATG+TGG + chr4.3:1426418-1426437 MS.gene020513:CDS 50.0%
AAGGAGACGGTCACTACTGA+AGG + chr4.3:1428141-1428160 MS.gene020513:CDS 50.0%
AGCCGGTAGCACAAAGATGT+GGG + chr4.3:1426419-1426438 MS.gene020513:CDS 50.0%
ATAAGTTCCTGCCATGGAGC+AGG - chr4.3:1426793-1426812 None:intergenic 50.0%
CACCCACATCTTTGTGCTAC+CGG - chr4.3:1426424-1426443 None:intergenic 50.0%
CCACCAAACGGATGCTTATC+TGG - chr4.3:1426219-1426238 None:intergenic 50.0%
CCAGATAAGCATCCGTTTGG+TGG + chr4.3:1426216-1426235 MS.gene020513:CDS 50.0%
GCTTTCTCAGCCACAACCTA+AGG - chr4.3:1426589-1426608 None:intergenic 50.0%
GGGAAGGCCTTAATGAACAG+AGG - chr4.3:1427541-1427560 None:intergenic 50.0%
GTGTGTGTGTGTGTGTGTCT+GGG - chr4.3:1426487-1426506 None:intergenic 50.0%
TGCCAAACTGCAAATCCGCA+AGG - chr4.3:1426093-1426112 None:intergenic 50.0%
TGCCTTGCGGATTTGCAGTT+TGG + chr4.3:1426088-1426107 MS.gene020513:intron 50.0%
TGGAGAAACCCCAGATCTCA+AGG + chr4.3:1426834-1426853 MS.gene020513:CDS 50.0%
TGTGTGTGTGTGTGTGTGTC+TGG - chr4.3:1426488-1426507 None:intergenic 50.0%
TTACTTACCGGGAACATGGG+AGG - chr4.3:1425201-1425220 None:intergenic 50.0%
TTAGATGCTGAGGCTCCTCT+TGG + chr4.3:1427904-1427923 MS.gene020513:CDS 50.0%
! GAGGATTCAGGTTCAAACCC+CGG - chr4.3:1427643-1427662 None:intergenic 50.0%
! GCTCTGTGTGTCATTGCACT+AGG + chr4.3:1427964-1427983 MS.gene020513:CDS 50.0%
! TCTTTTGGATGCTGCGAGCA+TGG + chr4.3:1426735-1426754 MS.gene020513:CDS 50.0%
!! CTGGTGCTGGTCACAAATGT+TGG - chr4.3:1425884-1425903 None:intergenic 50.0%
ATCTGCAGTGCAGTGCAGAG+AGG - chr4.3:1425629-1425648 None:intergenic 55.0%
GTCTGGGAGAGGAGTCATGA+AGG - chr4.3:1426471-1426490 None:intergenic 55.0%
TGTGTGTGTGTGTCTGGGAG+AGG - chr4.3:1426482-1426501 None:intergenic 55.0%
CTCTACACCTGCTCCATGGC+AGG + chr4.3:1426783-1426802 MS.gene020513:CDS 60.0%
GATGCTGCGAGCATGGCAGC+TGG + chr4.3:1426742-1426761 MS.gene020513:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 1424864 1428167 1424864 ID=MS.gene020513
chr4.3 mRNA 1424864 1428167 1424864 ID=MS.gene020513.t1;Parent=MS.gene020513
chr4.3 exon 1424864 1425212 1424864 ID=MS.gene020513.t1.exon1;Parent=MS.gene020513.t1
chr4.3 CDS 1424864 1425212 1424864 ID=cds.MS.gene020513.t1;Parent=MS.gene020513.t1
chr4.3 exon 1425383 1425549 1425383 ID=MS.gene020513.t1.exon2;Parent=MS.gene020513.t1
chr4.3 CDS 1425383 1425549 1425383 ID=cds.MS.gene020513.t1;Parent=MS.gene020513.t1
chr4.3 exon 1426147 1426446 1426147 ID=MS.gene020513.t1.exon3;Parent=MS.gene020513.t1
chr4.3 CDS 1426147 1426446 1426147 ID=cds.MS.gene020513.t1;Parent=MS.gene020513.t1
chr4.3 exon 1426592 1426855 1426592 ID=MS.gene020513.t1.exon4;Parent=MS.gene020513.t1
chr4.3 CDS 1426592 1426855 1426592 ID=cds.MS.gene020513.t1;Parent=MS.gene020513.t1
chr4.3 exon 1427790 1428167 1427790 ID=MS.gene020513.t1.exon5;Parent=MS.gene020513.t1
chr4.3 CDS 1427790 1428167 1427790 ID=cds.MS.gene020513.t1;Parent=MS.gene020513.t1
Gene Sequence

>MS.gene020513

ATGGTTATAGTTGGTGGTGGTAGTTGGAAAACGGCGATGTTAATGTCATCGTCGTTGAGTCACGTGCGTCCACTAACATGCGCATCTTCATCTTCATCTTCATCTTCATCACGAATAGCTCCTCATCCACACGATCTCATCAAATGGGTAACAAGAGAAGGTGGATTCGTTCACCGTGCTGTGAAGATAGCACAACTCGATTCCTCAAATGGTTTAGGGTTAGTGGCTAAGGAAGATATCCCAATTGGTACTGACCTAATTGCTCTTCCTCAACACATTCCTCTGCACTTCAACGATGATGATCGCAACTCTCACTCTCTCCTCCTTCAATTAACCTCCCATGTTCCCGGTAAGTAAAATTACCAGCTCAATTAGCTCATGAATTTGTATCTGTATGATAAGAAGAATTTATGTTATAAGCATTTAATTAAATGTTTATCCAAACAAGACTCTAGAAAGAAATAGTATTGAGTTGTCGTCGATGTTGAATAAATTGGACAATGTGGTAGCATTTGGCAGATGAATTGTGGGCAATGAAACTGGGTTTGAAGCTTCTTCATGAAAGGGCAAAACTTGGATCTTTTTGGTGGCCATACATCAGTAATCTCCCACAAACTTACACTCTTCCTATTTTCTTTCCTGGGGAAGACATCAAGAATTTGCACTATGCTCCTATTCTTCACCAGGTACACTTTACTTACTGTTAATCATTTTCGACTATACTATGTTATATGAAAAAAAAATTAGTATAATGCTTTGTGGCCTCTCTGCACTGCACTGCAGATTGCTAATCAAGAAAAGAAAAATGCAATTTTGTTCATCTTCATTTTGACTAGAGAAATGCATATAAGTATACGGCATATAATTAAGAATCTGTTGAACTATGATTTTTGTGTAAAAACCAGTGGTAAAATTCGGTGTAAATAATGTTGGTAGGTTTTTATTACTTCTCTCAGTCCTCACAAGAGCAATGTGCAACTTGTTCTCTTTGAAACATTTGAAATATTCTTAAGATATCCAACATTTGTGACCAGCACCAGAAGCTACAATTGTCTATCTCAGTGTCTATTAATTATGTGGCTTAATTGCACTTTTGACTCCTAATTATCACGATTTAGTTATTTTTGTCCCCCACTTTTGAAATGAGCAATTTGGATCCGCATAGTTTATCTCCAAGTGTAGTTTTGGGGTGTCAAAAATGCAATTAAGTCTAATTATTTTGAATGCCTTGCGGATTTGCAGTTTGGCAGCTAATTTTGTCAAATTCATGAATCACGAGACAGGTAAACAAAAGATGCCGATTTCTTCTTGATTTTGAGCAAAAAGTCAAGCATGCATTAGTCGGTCTTACACCAGATAAGCATCCGTTTGGTGGCCAAGAAGTAGATGCTTCATCTCTTGGATGGGCGATGTCAGCAGTTTCATCAAGAGCATTCAAGTTACATGGTAACAAGCAATCAAATGGGATAAACTTTGACATTCCCATGATGCTCCCTCTAATTGACATGTGTAATCATAGTTTCAATCCAAATGCTAGAATTGTTCAGGAACAAGAAGCCGGTAGCACAAAGATGTGGGTGAAGGCATGTTATTTCATACTCTTTCCTTCATGACTCCTCTCCCAGACACACACACACACACACACACACGCTCACACGTCAAATTCTGCTTTTAGAAACAACTATTCTTTAGATTTTTCTTTGCTGAGAAATTCAATATTTACCTTAGGTTGTGGCTGAGAAAGCAATCAAGGAAGATGACCCTTTATTACTTTGCTATGGATGTTTAAGCAATGATCTTTTCCTTCTTGATTATGGATTTGTGATACAGTCAAACCCTTATGACTGTATCGAGCTCAAATATGATGGTGCTCTTTTGGATGCTGCGAGCATGGCAGCTGGAGTTTCTTCACCAAATTTCTCTACACCTGCTCCATGGCAGGAACTTATTCTATCTCATCTAAATCTAGCTGGAGAAACCCCAGATCTCAAGGTATCTTCCACAAAATTCTATAGCTGTATTTAATTACTACTATTTAAAAATGAAAACTACTATTGGATGTTAAATAAGTGCTCTTACTCTTTCTATTAAAGAAATGACATTTTGACACACAAATTTGACATAAAATTTTGACAAATATTTAGAATGTTAGCGCTGTTAAAGTATGTGGTTAATTACGTCCATTGAATGGTTAATAAATATAAATATAACAAATTGTTGCAATTCATTAATGATATACTCAACACCGTTAACGACCATTTTGAATGTCATTAATCTCAATGTTCATTTGAGTATGTTTGAACACAAATTGTGGTTAATGAATATCATATATAAATATTTGAACACCTTAAAATCATGTATATTTGAATCCAGTTTGGATGATTAATATATGGATATATGAATATATGTGTTTGTGTGTGTTGGAAAACCTGAAAAATTATGTATAATGACGTTCAAAATCGCAGTTTATTTTCATCAATCATCTAATGAATGTAATTGACCATATATACTTAACGATCTAAATGTTTAATTTTGATGTCAAGTTTGTATGTCAAAAAAACACAACTCTTTCTATTATATAAGCTTACTGTACTTTGCAGAAAGGTTTAATCTCTTCTTTGTAGATGCATCAATACTACGTCCATAATCCATTATTTTTACGCATTATGTTTTCCCTCTGTTCATTAAGGCCTTCCCATAGAATAACTATGAACTTTCATAGTAATGTTTTTATAGCAAATATTGGTAATTAGTTATATTAGTATTGTCTCTTTCACCGGGGTTTGAACCTGAATCCTCTTAACTTATTCAACCCTTGGCTCAAATCAGTTGTGCTACTCAACTTCCCAAAACTTTCATATCAATTGGTTGATGTTAGCACAAGAGCCATAAGGTCATAATTTGATTTACTTTTTTCATGTAACAGGTGAGCTTAGGTGGGCAAGAAATAATAGAAGGTCGTTTGTTGGCTGCACTAAGAGTACTCCTTGCAAGTGATATGGCAAGTGTACAAAAGCATGACCTCAACACACTTAAGTCTTTAGATGCTGAGGCTCCTCTTGGTGTTGCAAATGACCTTGCTGTATTTCGTACCTTGATTGCTCTGTGTGTCATTGCACTAGGACACTTTCCCACCAAATTAATGGATGATGAATCTTTATTGAAGCAGGGTGCTTCAGGTTCAACAGAATTAGCCATTCAGTTCAGAATCCAAAAGAAGTCTGTAATTATAGATGTAATGAAAAACCTCTCAAGGAAGGTAAAGCTTCTTTCATCAAAGGAGACGGTCACTACTGAAGGCTAA
Protein sequence

>MS.gene020513.t1

MVIVGGGSWKTAMLMSSSLSHVRPLTCASSSSSSSSSRIAPHPHDLIKWVTREGGFVHRAVKIAQLDSSNGLGLVAKEDIPIGTDLIALPQHIPLHFNDDDRNSHSLLLQLTSHVPDELWAMKLGLKLLHERAKLGSFWWPYISNLPQTYTLPIFFPGEDIKNLHYAPILHQVNKRCRFLLDFEQKVKHALVGLTPDKHPFGGQEVDASSLGWAMSAVSSRAFKLHGNKQSNGINFDIPMMLPLIDMCNHSFNPNARIVQEQEAGSTKMWVKVVAEKAIKEDDPLLLCYGCLSNDLFLLDYGFVIQSNPYDCIELKYDGALLDAASMAAGVSSPNFSTPAPWQELILSHLNLAGETPDLKVSLGGQEIIEGRLLAALRVLLASDMASVQKHDLNTLKSLDAEAPLGVANDLAVFRTLIALCVIALGHFPTKLMDDESLLKQGASGSTELAIQFRIQKKSVIIDVMKNLSRKVKLLSSKETVTTEG