AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020685

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020685.t1 MTR_5g060780 71.471 340 40 4 40 332 17 346 1.41e-158 452
MS.gene020685.t1 MTR_5g061890 71.471 340 40 4 40 332 17 346 4.29e-158 451
MS.gene020685.t1 MTR_5g460920 71.176 340 41 4 40 332 17 346 8.84e-157 447
MS.gene020685.t1 MTR_5g060630 43.580 514 132 12 41 404 18 523 1.86e-107 326
MS.gene020685.t1 MTR_5g460740 43.580 514 132 12 41 404 18 523 1.86e-107 326
MS.gene020685.t1 MTR_5g061220 43.460 474 132 13 40 399 24 475 2.65e-102 313
MS.gene020685.t1 MTR_1g058210 45.606 421 141 13 39 404 16 403 1.50e-95 294
MS.gene020685.t1 MTR_2g024430 40.347 461 160 16 40 404 25 466 9.89e-84 265
MS.gene020685.t1 MTR_5g040880 35.684 468 184 12 40 404 16 469 5.74e-70 229
MS.gene020685.t1 MTR_5g082140 41.049 324 125 9 41 310 18 329 3.19e-64 213
MS.gene020685.t1 MTR_2g006380 42.368 321 113 12 40 306 25 327 1.26e-63 211
MS.gene020685.t1 MTR_2g006270 39.467 375 158 13 40 362 18 375 2.32e-62 207
MS.gene020685.t1 MTR_5g040740 43.450 313 107 10 40 297 17 314 3.11e-62 206
MS.gene020685.t1 MTR_5g060770 50.746 268 44 7 84 309 7 228 4.49e-58 194
MS.gene020685.t1 MTR_5g062960 50.532 188 67 4 209 374 1 184 2.32e-45 155
MS.gene020685.t1 MTR_3g073420 29.757 494 191 17 41 396 25 500 1.53e-43 160
MS.gene020685.t1 MTR_5g460910 40.469 341 96 15 74 350 13 310 1.10e-42 152
MS.gene020685.t1 MTR_5g062970 46.597 191 48 6 39 178 42 229 1.11e-40 144
MS.gene020685.t1 MTR_2g094570 30.833 360 147 11 40 307 16 365 3.54e-39 149
MS.gene020685.t1 MTR_2g094570 30.833 360 147 11 40 307 16 365 3.73e-39 149
MS.gene020685.t1 MTR_2g094570 30.833 360 147 11 40 307 72 421 3.84e-39 149
MS.gene020685.t1 MTR_4g058930 31.967 366 141 11 40 307 17 372 2.89e-38 146
MS.gene020685.t1 MTR_4g058930 31.967 366 141 11 40 307 17 372 4.03e-38 145
MS.gene020685.t1 MTR_1g094960 30.447 358 150 10 40 307 24 372 1.18e-37 144
MS.gene020685.t1 MTR_1g064430 30.978 368 144 11 40 307 32 389 1.72e-37 144
MS.gene020685.t1 MTR_1g064430 30.978 368 144 11 40 307 45 402 1.88e-37 144
MS.gene020685.t1 MTR_2g005240 30.061 326 167 11 40 310 28 347 7.88e-30 122
MS.gene020685.t1 MTR_8g100050 28.097 331 161 9 40 306 64 381 5.61e-29 119
MS.gene020685.t1 MTR_8g100050 27.401 354 169 10 40 329 64 393 8.41e-29 119
MS.gene020685.t1 MTR_7g101280 26.667 375 152 11 41 307 37 396 9.42e-28 115
MS.gene020685.t1 MTR_0041s0110 26.667 375 152 11 41 307 37 396 9.42e-28 115
MS.gene020685.t1 MTR_7g062540 27.586 348 180 12 40 332 19 349 2.87e-26 111
MS.gene020685.t1 MTR_7g101275 27.329 322 153 10 45 302 2 306 5.28e-26 108
MS.gene020685.t1 MTR_4g021580 27.378 347 180 11 41 332 25 354 1.51e-25 109
MS.gene020685.t1 MTR_4g021580 27.378 347 180 11 41 332 25 354 1.54e-25 109
MS.gene020685.t1 MTR_2g018690 27.900 319 166 9 40 300 28 340 2.07e-24 106
MS.gene020685.t1 MTR_8g027440 26.761 355 173 13 40 332 34 363 1.23e-23 103
MS.gene020685.t1 MTR_5g076270 27.586 319 167 9 40 300 29 341 2.10e-23 103
MS.gene020685.t1 MTR_2g043250 28.213 319 163 12 40 300 28 338 2.63e-23 102
MS.gene020685.t1 MTR_1g024025 27.174 368 191 14 26 334 34 383 3.04e-23 102
MS.gene020685.t1 MTR_3g064050 25.882 425 220 14 40 397 31 427 3.76e-23 102
MS.gene020685.t1 MTR_8g079492 26.814 317 171 7 40 300 28 339 1.59e-22 100
MS.gene020685.t1 MTR_8g079492 26.814 317 171 7 40 300 28 339 1.62e-22 100
MS.gene020685.t1 MTR_4g124900 27.044 318 168 10 40 300 28 338 2.08e-22 100
MS.gene020685.t1 MTR_4g124900 27.044 318 168 10 40 300 28 338 2.09e-22 100
MS.gene020685.t1 MTR_0041s0120 26.205 332 156 10 45 302 2 318 6.54e-22 97.1
MS.gene020685.t1 MTR_2g093740 26.124 356 185 11 40 331 61 402 1.10e-20 94.7
MS.gene020685.t1 MTR_2g093740 26.124 356 185 11 40 331 61 402 1.35e-20 94.4
MS.gene020685.t1 MTR_2g014770 25.284 352 187 12 40 332 58 392 1.57e-20 94.4
MS.gene020685.t1 MTR_2g093740 26.124 356 185 11 40 331 61 402 1.65e-20 94.4
MS.gene020685.t1 MTR_2g014770 25.284 352 187 12 40 332 58 392 1.75e-20 94.0
MS.gene020685.t1 MTR_4g088210 24.167 360 185 11 40 332 43 381 1.85e-19 90.9
MS.gene020685.t1 MTR_4g088210 24.167 360 185 11 40 332 43 381 2.08e-19 90.9
MS.gene020685.t1 MTR_8g101360 26.730 318 171 9 40 300 36 348 2.89e-19 90.9
MS.gene020685.t1 MTR_8g079492 30.144 209 120 8 100 300 67 257 1.23e-13 73.2
MS.gene020685.t1 MTR_4g060460 30.108 186 115 6 122 300 202 379 2.63e-13 72.0
MS.gene020685.t1 MTR_4g060460 30.108 186 115 6 122 300 202 379 2.67e-13 72.0
MS.gene020685.t1 MTR_2g093740 28.378 222 138 8 117 331 65 272 1.07e-12 70.1
MS.gene020685.t1 MTR_2g093740 29.493 217 128 9 122 331 217 415 1.09e-12 70.1
MS.gene020685.t1 MTR_2g093740 29.493 217 128 9 122 331 217 415 1.27e-12 70.1
MS.gene020685.t1 MTR_2g093740 28.378 222 138 8 117 331 65 272 1.30e-12 70.1
MS.gene020685.t1 MTR_2g093740 29.493 217 128 9 122 331 217 415 1.47e-12 69.7
MS.gene020685.t1 MTR_2g093740 29.493 217 128 9 122 331 87 285 1.55e-12 69.7
MS.gene020685.t1 MTR_2g093740 29.493 217 128 9 122 331 87 285 1.76e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020685.t1 AT1G77850 31.781 365 152 12 30 307 13 367 2.67e-47 170
MS.gene020685.t1 AT1G77850 31.781 365 152 12 30 307 13 367 2.77e-47 171
MS.gene020685.t1 AT4G30080 30.357 392 171 11 40 340 24 404 2.48e-36 140
MS.gene020685.t1 AT2G46530 31.077 325 161 10 40 309 26 342 7.99e-33 130
MS.gene020685.t1 AT2G46530 31.077 325 161 10 40 309 47 363 1.23e-32 130
MS.gene020685.t1 AT3G61830 29.573 328 162 11 40 309 30 346 2.41e-31 126
MS.gene020685.t1 AT2G28350 28.647 377 140 13 40 307 15 371 8.39e-29 119
MS.gene020685.t1 AT1G59750 29.467 319 164 11 40 303 27 339 9.11e-29 119
MS.gene020685.t1 AT1G59750 29.467 319 164 11 40 303 27 339 9.50e-29 119
MS.gene020685.t1 AT2G28350 28.647 377 140 13 40 307 15 371 9.64e-29 119
MS.gene020685.t1 AT1G59750 29.467 319 164 11 40 303 27 339 1.08e-28 118
MS.gene020685.t1 AT1G59750 29.467 319 164 11 40 303 27 339 1.08e-28 118
MS.gene020685.t1 AT5G62000 27.174 368 170 14 40 336 66 406 1.64e-27 115
MS.gene020685.t1 AT5G62000 27.174 368 170 14 40 336 66 406 1.64e-27 115
MS.gene020685.t1 AT5G62000 27.174 368 170 14 40 336 66 406 1.64e-27 115
MS.gene020685.t1 AT5G62000 27.174 368 170 14 40 336 66 406 1.64e-27 115
MS.gene020685.t1 AT5G62000 27.174 368 170 14 40 336 66 406 1.81e-27 115
MS.gene020685.t1 AT4G23980 27.914 326 171 10 40 309 17 334 2.43e-26 111
MS.gene020685.t1 AT4G23980 27.914 326 171 10 40 309 17 334 2.51e-26 111
MS.gene020685.t1 AT5G37020 28.213 319 165 9 40 300 27 339 7.50e-26 110
MS.gene020685.t1 AT5G37020 28.213 319 165 9 40 300 27 339 8.48e-26 110
MS.gene020685.t1 AT1G30330 28.302 318 166 8 40 300 30 342 2.04e-25 109
MS.gene020685.t1 AT1G30330 28.302 318 166 8 40 300 30 342 2.04e-25 109
MS.gene020685.t1 AT1G30330 28.302 318 166 8 40 300 28 340 2.22e-25 109
MS.gene020685.t1 AT1G34390 27.950 322 151 10 40 300 32 333 1.91e-24 105
MS.gene020685.t1 AT1G35540 28.395 324 149 11 40 300 32 335 5.20e-24 104
MS.gene020685.t1 AT1G34410 28.049 328 145 12 40 300 32 335 1.94e-23 102
MS.gene020685.t1 AT1G34310 28.354 328 144 12 40 300 32 335 1.95e-23 102
MS.gene020685.t1 AT1G35240 28.571 329 142 13 40 300 32 335 4.09e-23 102
MS.gene020685.t1 AT1G35520 28.354 328 144 12 40 300 32 335 6.21e-23 101
MS.gene020685.t1 AT1G19850 27.523 327 173 10 40 308 48 368 9.78e-21 95.1
MS.gene020685.t1 AT1G19850 27.523 327 173 10 40 308 59 379 1.06e-20 95.1
MS.gene020685.t1 AT3G61830 27.891 294 147 10 70 309 13 295 4.02e-20 92.8
MS.gene020685.t1 AT1G19220 27.187 320 165 11 40 300 28 338 5.42e-20 92.8
MS.gene020685.t1 AT2G46530 33.846 195 110 7 122 309 73 255 1.76e-19 90.9
MS.gene020685.t1 AT2G33860 26.444 329 158 14 40 300 59 371 1.40e-16 82.0
MS.gene020685.t1 AT5G20730 25.767 326 163 12 40 300 29 340 1.83e-16 82.0
MS.gene020685.t1 AT5G20730 26.380 326 161 13 40 300 29 340 2.39e-16 82.0
MS.gene020685.t1 AT5G20730 26.687 326 159 14 40 300 29 339 6.30e-16 80.5
MS.gene020685.t1 AT5G37020 31.551 187 111 7 122 300 67 244 1.39e-15 79.3
MS.gene020685.t1 AT2G33860 30.481 187 112 7 122 300 38 214 1.44e-15 78.6
MS.gene020685.t1 AT5G60450 29.412 187 113 8 122 300 213 388 2.38e-13 72.4
MS.gene020685.t1 AT5G60450 29.412 187 113 8 122 300 189 364 2.46e-13 72.4

Find 117 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTTATCATAGGTTAAC+TGG 0.215844 5.4:-74232407 MS.gene020685:intron
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.4:+74234445 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.4:-74233178 MS.gene020685:CDS
TTGGCTCATTGGACCTATTA+TGG 0.290349 5.4:+74231934 None:intergenic
GGTTTGGATTCTGTGGGATA+TGG 0.299940 5.4:+74232346 None:intergenic
ACCTCAAGCATGCGCCAATT+AGG 0.307618 5.4:+74233164 None:intergenic
TTTGGATTCTGTGGGATATT+AGG 0.307838 5.4:+74232378 None:intergenic
TCACTTGGTGCTTGGCTCAT+TGG 0.308376 5.4:+74231923 None:intergenic
GGGATATGGTTTGGATGGTT+TGG 0.314165 5.4:+74232360 None:intergenic
TGCCAAGTATTGACTAGGTT+TGG 0.319081 5.4:+74232330 None:intergenic
CTACTTCCCACAAGGTCATT+TGG 0.321701 5.4:-74234422 MS.gene020685:CDS
CGCCGTTACGCCGTGTATTC+CGG 0.325201 5.4:-74234889 MS.gene020685:intron
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5.4:-74233296 MS.gene020685:CDS
CGTTGCCTCATAACCATAAT+AGG 0.348120 5.4:-74231947 MS.gene020685:CDS
CTTGGTCAGTTACGGGAGTT+AGG 0.352495 5.4:+74234266 None:intergenic
ATGGTGATTGTGGCAGCGTT+AGG 0.364809 5.4:+74234382 None:intergenic
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5.4:+74233165 None:intergenic
ACAAAAGAGATTGTCCCTTT+AGG 0.373244 5.4:+74233209 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5.4:+74234460 None:intergenic
GGATTCTGTGGGATATGGTT+TGG 0.376628 5.4:+74232351 None:intergenic
ATGAATCTGATACAGATAAA+TGG 0.381071 5.4:+74232084 None:intergenic
CCACAGGAGGAGGAGGTTCT+TGG 0.381303 5.4:+74234248 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5.4:+74233298 None:intergenic
GATAAGCATGACGGATTCAC+TGG 0.385768 5.4:-74232047 MS.gene020685:CDS
AGGAGGTTCTTGGTCAGTTA+CGG 0.395498 5.4:+74234258 None:intergenic
CTCTTTATCATAGGTTAACT+GGG 0.403608 5.4:-74232406 MS.gene020685:intron
GGAGGTTCTTGGTCAGTTAC+GGG 0.408872 5.4:+74234259 None:intergenic
CGCTTCATCTGTAGTGAACT+TGG 0.416879 5.4:+74233466 None:intergenic
TCTGTGGGATATGGTTTGGA+TGG 0.420148 5.4:+74232355 None:intergenic
TACATCGTCGACTTCTTGAC+TGG 0.422976 5.4:+74234222 None:intergenic
TTCCTCCTTCATTCCTATGC+AGG 0.431062 5.4:-74232126 MS.gene020685:CDS
TGGTGATTGTGGCAGCGTTA+GGG 0.433150 5.4:+74234383 None:intergenic
TATAAGTGTACCTCCATTCT+CGG 0.441745 5.4:+74233621 None:intergenic
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5.4:+74234465 None:intergenic
TCATTCCTATGCAGGGAGTC+AGG 0.445797 5.4:-74232118 MS.gene020685:CDS
TTGGACCTATTATGGTTATG+AGG 0.446632 5.4:+74231942 None:intergenic
TTGGATGGTTTGGATTCTGT+GGG 0.448826 5.4:+74232370 None:intergenic
TACGGGAGTTAGGAGCAGTT+TGG 0.459974 5.4:+74234276 None:intergenic
TTTGGATGGTTTGGATTCTG+TGG 0.470031 5.4:+74232369 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5.4:-74233308 MS.gene020685:CDS
AATGAAAACAACAGAATCGT+TGG 0.478070 5.4:+74233532 None:intergenic
ACTGGGAATAATTCAATGGC+AGG 0.481964 5.4:-74232029 MS.gene020685:CDS
CAACCTGCCAAGTATTGACT+AGG 0.486943 5.4:+74232325 None:intergenic
GAAGATTCATCTTTCTTCAA+AGG 0.488166 5.4:+74233257 None:intergenic
TGACATATTCTCAGCCTAAA+GGG 0.490948 5.4:-74233223 MS.gene020685:CDS
ATGACATATTCTCAGCCTAA+AGG 0.493756 5.4:-74233224 MS.gene020685:CDS
ATCCAAACCTAGTCAATACT+TGG 0.494257 5.4:-74232332 MS.gene020685:CDS
CAGTTTGGCAAAGACCTCTT+CGG 0.495832 5.4:+74234291 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.500782 5.4:-74232402 MS.gene020685:intron
TTGACTAGGTTTGGATTCTG+TGG 0.502251 5.4:+74232339 None:intergenic
TGACTAGGTTTGGATTCTGT+GGG 0.503890 5.4:+74232340 None:intergenic
TCTGCTGGAAAGATATTTGT+CGG 0.504917 5.4:-74233503 MS.gene020685:CDS
AAGATACCTCACTTGGTGCT+TGG 0.507669 5.4:+74231915 None:intergenic
GACTCCCTGCATAGGAATGA+AGG 0.513722 5.4:+74232121 None:intergenic
AGATATTTGTCGGAATTCGC+AGG 0.516804 5.4:-74233493 MS.gene020685:CDS
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5.4:-74233165 MS.gene020685:intron
ACAGATGAAGCGGGTAAAGA+AGG 0.527655 5.4:-74233455 MS.gene020685:CDS
TCCTCCTTCATTCCTATGCA+GGG 0.528220 5.4:-74232125 MS.gene020685:CDS
GGCAGAGACGATGCATGGAA+TGG 0.529105 5.4:+74234339 None:intergenic
GAGACGATGCATGGAATGGA+TGG 0.532282 5.4:+74234343 None:intergenic
ACTTCTTGACTGGGAACCAC+AGG 0.539067 5.4:+74234232 None:intergenic
CCAAGGTTTAGCTCTATGTC+AGG 0.539866 5.4:+74231990 None:intergenic
AAGGAGGAAGTTGACGGTGG+AGG 0.544243 5.4:+74232140 None:intergenic
AAACTCAGTAGATTACATAC+TGG 0.547036 5.4:-74233590 MS.gene020685:CDS
CCGACCTTAAAAGCCGAGAA+TGG 0.547731 5.4:-74233634 MS.gene020685:CDS
AAAATATGGTAGAAATGCGC+CGG 0.548670 5.4:-74234484 MS.gene020685:intron
AAACCTAGTCAATACTTGGC+AGG 0.550621 5.4:-74232328 MS.gene020685:intron
CCAAGAACCTCCTCCTCCTG+TGG 0.552145 5.4:-74234248 MS.gene020685:CDS
AATTCAATGGCAGGGTTGAG+TGG 0.552586 5.4:-74232020 MS.gene020685:CDS
TACACGGCGTAACGGCGGAA+TGG 0.554200 5.4:+74234895 None:intergenic
ATTCACTGGGAATAATTCAA+TGG 0.557749 5.4:-74232033 MS.gene020685:CDS
ACGATGCCATGAAGATTGGT+TGG 0.558168 5.4:-74233304 MS.gene020685:CDS
CTGGGAACCACAGGAGGAGG+AGG 0.561947 5.4:+74234241 None:intergenic
GTGTATTATCCGACAGCTAG+TGG 0.562344 5.4:-74233362 MS.gene020685:CDS
GCAAAGACCTCTTCGGTGTG+AGG 0.573732 5.4:+74234298 None:intergenic
CATGCTCCAAATGACCTTGT+GGG 0.574839 5.4:+74234416 None:intergenic
TCAGTAGATTACATACTGGC+TGG 0.577585 5.4:-74233586 MS.gene020685:CDS
TGACTGGGAACCACAGGAGG+AGG 0.579874 5.4:+74234238 None:intergenic
GAATGAGTTCACGAGACAGT+TGG 0.580016 5.4:-74234952 MS.gene020685:CDS
TCATGCCTGACTCCCTGCAT+AGG 0.582427 5.4:+74232113 None:intergenic
CCTGACATAGAGCTAAACCT+TGG 0.585110 5.4:-74231990 MS.gene020685:CDS
TGGACGGTAACTATCAAGAA+TGG 0.587230 5.4:+74234363 None:intergenic
ACTATCAAGAATGGTGATTG+TGG 0.590559 5.4:+74234372 None:intergenic
ATGAAGGAGGAAGTTGACGG+TGG 0.590817 5.4:+74232137 None:intergenic
GGTGATTGTGGCAGCGTTAG+GGG 0.592113 5.4:+74234384 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.598930 5.4:-74233465 MS.gene020685:CDS
TGTTGATCCTCACACCGAAG+AGG 0.601436 5.4:-74234305 MS.gene020685:CDS
TCAACGGCAGAGACGATGCA+TGG 0.604313 5.4:+74234334 None:intergenic
GCCAATGAAGATACCTCACT+TGG 0.608165 5.4:+74231908 None:intergenic
CTGGGAATAATTCAATGGCA+GGG 0.616068 5.4:-74232028 MS.gene020685:CDS
GCAGAGCCTACCGGAATACA+CGG 0.616741 5.4:+74234879 None:intergenic
CGATGCATGGAATGGATGGA+CGG 0.620709 5.4:+74234347 None:intergenic
ACATCGTCGACTTCTTGACT+GGG 0.620754 5.4:+74234223 None:intergenic
GCATGCTCCAAATGACCTTG+TGG 0.621993 5.4:+74234415 None:intergenic
CTAAACCTTGGCAGTCCTAG+AGG 0.624031 5.4:-74231978 MS.gene020685:CDS
TACCGGAATACACGGCGTAA+CGG 0.624639 5.4:+74234887 None:intergenic
GATGAAGCGGGTAAAGAAGG+AGG 0.625313 5.4:-74233452 MS.gene020685:CDS
GGAATGAAGGAGGAAGTTGA+CGG 0.626658 5.4:+74232134 None:intergenic
TAATCTACTGAGTTTCGCGT+AGG 0.626849 5.4:+74233598 None:intergenic
CAGCACCTCTAGGACTGCCA+AGG 0.629336 5.4:+74231973 None:intergenic
TCTTGACTGGGAACCACAGG+AGG 0.630072 5.4:+74234235 None:intergenic
AATCTTTGGAATCTTGACGT+CGG 0.630358 5.4:+74234459 None:intergenic
ATAAGCATGACGGATTCACT+GGG 0.631573 5.4:-74232046 MS.gene020685:CDS
AAGTTCACTACAGATGAAGC+GGG 0.636021 5.4:-74233464 MS.gene020685:CDS
GGCAACGAGTCAGCACCTCT+AGG 0.637822 5.4:+74231963 None:intergenic
ACCTTAAAAGCCGAGAATGG+AGG 0.638927 5.4:-74233631 MS.gene020685:CDS
TTTAGATGCAGCGGAACTGG+CGG 0.641845 5.4:-74233408 MS.gene020685:CDS
ATGAAGCGGGTAAAGAAGGA+GGG 0.650423 5.4:-74233451 MS.gene020685:CDS
TCACGGCAACCACTAGCTGT+CGG 0.652491 5.4:+74233353 None:intergenic
AATGAGCCAAGCACCAAGTG+AGG 0.655123 5.4:-74231921 None:intergenic
AGGATCAACAAAGAGATCAA+CGG 0.658354 5.4:+74234318 None:intergenic
CGGAATACACGGCGTAACGG+CGG 0.659535 5.4:+74234890 None:intergenic
TAGCATCCACCACAAAATCA+CGG 0.661399 5.4:+74233336 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5.4:+74234461 None:intergenic
AAAGTTTACTACTTCCCACA+AGG 0.678948 5.4:-74234430 MS.gene020685:CDS
TCCCTGCATAGGAATGAAGG+AGG 0.687917 5.4:+74232124 None:intergenic
ACGTATAGCGAAGAAATGCA+CGG 0.718371 5.4:-74234976 MS.gene020685:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATCAAATAATCTTATTA+TGG + chr5.4:74233846-74233865 None:intergenic 15.0%
!!! ATTTTTTATCTCTTTATCAT+AGG - chr5.4:74234489-74234508 MS.gene020685:intron 15.0%
!!! CTTAATTTTCAAATTACATT+TGG + chr5.4:74233961-74233980 None:intergenic 15.0%
!! ATAGCTCACTATATTTATTT+AGG - chr5.4:74233097-74233116 MS.gene020685:intron 20.0%
!! CTAAAATTATGAAACTTAGT+AGG - chr5.4:74233908-74233927 MS.gene020685:intron 20.0%
!! TCTTATTATGGATTCAAATT+TGG + chr5.4:74233834-74233853 None:intergenic 20.0%
! ATAAGAATTTGGTACGTATA+GGG + chr5.4:74233165-74233184 None:intergenic 25.0%
! ATGAATCTGATACAGATAAA+TGG + chr5.4:74234823-74234842 None:intergenic 25.0%
! CACATAATTCTTGTAATCAA+GGG - chr5.4:74232831-74232850 MS.gene020685:intron 25.0%
! CTCATGTTAACTTAAAAACT+TGG - chr5.4:74232211-74232230 MS.gene020685:intron 25.0%
! GGAATTATTAATGTTAGAGA+TGG + chr5.4:74234008-74234027 None:intergenic 25.0%
! TAGGGAGTTACATATAAAAA+CGG + chr5.4:74234122-74234141 None:intergenic 25.0%
! TCACATAATTCTTGTAATCA+AGG - chr5.4:74232830-74232849 MS.gene020685:intron 25.0%
! TTACAAGAATTATGTGACTA+TGG + chr5.4:74232827-74232846 None:intergenic 25.0%
!! ATGGATTAAGCTTATAATTG+AGG + chr5.4:74232246-74232265 None:intergenic 25.0%
!!! GTACTTATGCTATCTTATTT+TGG - chr5.4:74233761-74233780 MS.gene020685:intron 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + chr5.4:74232462-74232481 None:intergenic 25.0%
AAACTCAGTAGATTACATAC+TGG - chr5.4:74233314-74233333 MS.gene020685:CDS 30.0%
AATGAAAACAACAGAATCGT+TGG + chr5.4:74233375-74233394 None:intergenic 30.0%
AGGGAGTTACATATAAAAAC+GGG + chr5.4:74234121-74234140 None:intergenic 30.0%
ATTCACTGGGAATAATTCAA+TGG - chr5.4:74234871-74234890 MS.gene020685:intron 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5.4:74233726-74233745 MS.gene020685:intron 30.0%
CTCTTTATCATAGGTTAACT+GGG - chr5.4:74234498-74234517 MS.gene020685:intron 30.0%
GAAACTTGGTAGAAGAAATT+AGG - chr5.4:74233054-74233073 MS.gene020685:intron 30.0%
GAAGATTCATCTTTCTTCAA+AGG + chr5.4:74233650-74233669 None:intergenic 30.0%
GATAAGAATTTGGTACGTAT+AGG + chr5.4:74233166-74233185 None:intergenic 30.0%
GCTTATAATTGAGGTAATCT+TGG + chr5.4:74232237-74232256 None:intergenic 30.0%
GTATTATTACTACTACGAAC+CGG - chr5.4:74233119-74233138 MS.gene020685:intron 30.0%
TAAGAATTTGGTACGTATAG+GGG + chr5.4:74233164-74233183 None:intergenic 30.0%
TACAATGAGAAATGCTTAGA+AGG + chr5.4:74234199-74234218 None:intergenic 30.0%
TCAAGATTCTACTTAGCATT+AGG + chr5.4:74234228-74234247 None:intergenic 30.0%
TCTCTTTATCATAGGTTAAC+TGG - chr5.4:74234497-74234516 MS.gene020685:intron 30.0%
TGTAATTTAGGCAGTGTAAT+TGG + chr5.4:74234743-74234762 None:intergenic 30.0%
TTAGAGAATGTGGAATTTGT+TGG + chr5.4:74234718-74234737 None:intergenic 30.0%
TTCAGTTATAGATGATTGCA+TGG + chr5.4:74232265-74232284 None:intergenic 30.0%
! GCTAAGTAGAATCTTGATTT+TGG - chr5.4:74234231-74234250 MS.gene020685:CDS 30.0%
! GTTTTCATTAAAAACTCTGC+TGG - chr5.4:74233386-74233405 MS.gene020685:CDS 30.0%
! TAGAGAATGTGGAATTTGTT+GGG + chr5.4:74234717-74234736 None:intergenic 30.0%
!! AAAGTCTAGGACTTTTTGTT+GGG + chr5.4:74234679-74234698 None:intergenic 30.0%
!! GTTTTGAATGATAAGCATGA+CGG - chr5.4:74234848-74234867 MS.gene020685:intron 30.0%
!!! CTTTTTTTTCAACCAATCAG+CGG + chr5.4:74232950-74232969 None:intergenic 30.0%
AAAATATGGTAGAAATGCGC+CGG - chr5.4:74232420-74232439 MS.gene020685:intron 35.0%
AAAGTTTACTACTTCCCACA+AGG - chr5.4:74232474-74232493 MS.gene020685:intron 35.0%
AAATGAATCACCATGAGTTG+AGG - chr5.4:74232993-74233012 MS.gene020685:intron 35.0%
ACAAAAGAGATTGTCCCTTT+AGG + chr5.4:74233698-74233717 None:intergenic 35.0%
ACTATCAAGAATGGTGATTG+TGG + chr5.4:74232535-74232554 None:intergenic 35.0%
AGAAGAACATGAACAAAGAG+TGG - chr5.4:74233024-74233043 MS.gene020685:intron 35.0%
AGATTTCCAACCAATCTTCA+TGG + chr5.4:74233609-74233628 None:intergenic 35.0%
AGGATCAACAAAGAGATCAA+CGG + chr5.4:74232589-74232608 None:intergenic 35.0%
ATCCAAACCTAGTCAATACT+TGG - chr5.4:74234572-74234591 MS.gene020685:intron 35.0%
ATCTATCAAGTGCAAGAACA+AGG + chr5.4:74234030-74234049 None:intergenic 35.0%
ATGACATATTCTCAGCCTAA+AGG - chr5.4:74233680-74233699 MS.gene020685:intron 35.0%
CTAAATTAGCCCACCTAAAT+TGG - chr5.4:74234274-74234293 MS.gene020685:CDS 35.0%
CTATTGCAGGGAGTAATATT+GGG + chr5.4:74232360-74232379 None:intergenic 35.0%
GTAATTGGCATTAGAGAATG+TGG + chr5.4:74234728-74234747 None:intergenic 35.0%
TACAAGCTTAGCAAACGAAA+AGG + chr5.4:74232050-74232069 None:intergenic 35.0%
TATAAGTGTACCTCCATTCT+CGG + chr5.4:74233286-74233305 None:intergenic 35.0%
TCTATCAAGTGCAAGAACAA+GGG + chr5.4:74234029-74234048 None:intergenic 35.0%
TCTGCTGGAAAGATATTTGT+CGG - chr5.4:74233401-74233420 MS.gene020685:CDS 35.0%
TGACATATTCTCAGCCTAAA+GGG - chr5.4:74233681-74233700 MS.gene020685:intron 35.0%
TTATCATAGGTTAACTGGGA+TGG - chr5.4:74234502-74234521 MS.gene020685:intron 35.0%
TTGGACCTATTATGGTTATG+AGG + chr5.4:74234965-74234984 None:intergenic 35.0%
TTTGGATTCTGTGGGATATT+AGG + chr5.4:74234529-74234548 None:intergenic 35.0%
! ACATTTGGAACTTATCCTAC+AGG + chr5.4:74233946-74233965 None:intergenic 35.0%
! AGACAGTTGGCACATTTTAA+TGG - chr5.4:74231965-74231984 MS.gene020685:CDS 35.0%
! ATGAAGATTGGTTGGAAATC+TGG - chr5.4:74233608-74233627 MS.gene020685:CDS 35.0%
! CATTTGGAACTTATCCTACA+GGG + chr5.4:74233945-74233964 None:intergenic 35.0%
! CGCATTTCTACCATATTTTG+GGG + chr5.4:74232419-74232438 None:intergenic 35.0%
! CGCTGATTGGTTGAAAAAAA+AGG - chr5.4:74232948-74232967 MS.gene020685:intron 35.0%
! GCGCATTTCTACCATATTTT+GGG + chr5.4:74232420-74232439 None:intergenic 35.0%
!! AAGTCTAGGACTTTTTGTTG+GGG + chr5.4:74234678-74234697 None:intergenic 35.0%
!! AATCTTTGGAATCTTGACGT+CGG + chr5.4:74232448-74232467 None:intergenic 35.0%
!! AGAGAATGTGGAATTTGTTG+GGG + chr5.4:74234716-74234735 None:intergenic 35.0%
!! GAAAGTCTAGGACTTTTTGT+TGG + chr5.4:74234680-74234699 None:intergenic 35.0%
!! TTGGCACATTTTAATGGCTT+TGG - chr5.4:74231971-74231990 MS.gene020685:CDS 35.0%
!!! GAAGTTGATTTTTTGTTGGG+GGG + chr5.4:74234654-74234673 None:intergenic 35.0%
!!! GGAAGTTGATTTTTTGTTGG+GGG + chr5.4:74234655-74234674 None:intergenic 35.0%
!!! GGGAAGTTGATTTTTTGTTG+GGG + chr5.4:74234656-74234675 None:intergenic 35.0%
!!! GGGGAAGTTGATTTTTTGTT+GGG + chr5.4:74234657-74234676 None:intergenic 35.0%
AAACCTAGTCAATACTTGGC+AGG - chr5.4:74234576-74234595 MS.gene020685:intron 40.0%
AAGTTCACTACAGATGAAGC+GGG - chr5.4:74233440-74233459 MS.gene020685:CDS 40.0%
ACGTATAGCGAAGAAATGCA+CGG - chr5.4:74231928-74231947 MS.gene020685:CDS 40.0%
ACTATTGCAGCAAGTCAAGT+AGG + chr5.4:74234141-74234160 None:intergenic 40.0%
ACTGGGAATAATTCAATGGC+AGG - chr5.4:74234875-74234894 MS.gene020685:intron 40.0%
AGAGTGGAGAATGAGAAACT+TGG - chr5.4:74233040-74233059 MS.gene020685:intron 40.0%
AGATATTTGTCGGAATTCGC+AGG - chr5.4:74233411-74233430 MS.gene020685:CDS 40.0%
ATCAAGGGAGAATTGCGAAT+TGG - chr5.4:74232846-74232865 MS.gene020685:intron 40.0%
ATCTCATGTTCGATTCTCTC+CGG + chr5.4:74234302-74234321 None:intergenic 40.0%
CAAGTTCACTACAGATGAAG+CGG - chr5.4:74233439-74233458 MS.gene020685:CDS 40.0%
CGTTGCCTCATAACCATAAT+AGG - chr5.4:74234957-74234976 MS.gene020685:CDS 40.0%
CTATTGCAGCAAGTCAAGTA+GGG + chr5.4:74234140-74234159 None:intergenic 40.0%
CTGGGAATAATTCAATGGCA+GGG - chr5.4:74234876-74234895 MS.gene020685:intron 40.0%
GAATCGAACATGAGATCTCA+AGG - chr5.4:74234306-74234325 MS.gene020685:CDS 40.0%
GAGCATAGTCACATAATTCG+CGG + chr5.4:74233235-74233254 None:intergenic 40.0%
GCTATTGCAGGGAGTAATAT+TGG + chr5.4:74232361-74232380 None:intergenic 40.0%
GTATTCATGATGCTCTCATG+AGG - chr5.4:74232067-74232086 MS.gene020685:CDS 40.0%
TAATCTACTGAGTTTCGCGT+AGG + chr5.4:74233309-74233328 None:intergenic 40.0%
TACGCGACAGGATAAGAATT+TGG + chr5.4:74233176-74233195 None:intergenic 40.0%
TAGCATCCACCACAAAATCA+CGG + chr5.4:74233571-74233590 None:intergenic 40.0%
TCAGTAGATTACATACTGGC+TGG - chr5.4:74233318-74233337 MS.gene020685:CDS 40.0%
TGACTAGGTTTGGATTCTGT+GGG + chr5.4:74234567-74234586 None:intergenic 40.0%
TGCCAAAATCAAACCCACTT+GGG + chr5.4:74234373-74234392 None:intergenic 40.0%
TGGACGGTAACTATCAAGAA+TGG + chr5.4:74232544-74232563 None:intergenic 40.0%
TTGACTAGGTTTGGATTCTG+TGG + chr5.4:74234568-74234587 None:intergenic 40.0%
TTGGCTCATTGGACCTATTA+TGG + chr5.4:74234973-74234992 None:intergenic 40.0%
TTGTGTGAGAGTCTTGACAT+AGG + chr5.4:74232721-74232740 None:intergenic 40.0%
! GAAGAACTCTGAGAAAGTCT+AGG + chr5.4:74234692-74234711 None:intergenic 40.0%
! GGCGCATTTCTACCATATTT+TGG + chr5.4:74232421-74232440 None:intergenic 40.0%
! TTGGATGGTTTGGATTCTGT+GGG + chr5.4:74234537-74234556 None:intergenic 40.0%
! TTTGGATGGTTTGGATTCTG+TGG + chr5.4:74234538-74234557 None:intergenic 40.0%
! TTTTGTTGGGGGGAAATTGA+TGG + chr5.4:74234644-74234663 None:intergenic 40.0%
!! AACCCAAGTGGGTTTGATTT+TGG - chr5.4:74234368-74234387 MS.gene020685:CDS 40.0%
!! AGAATGTGGAATTTGTTGGG+GGG + chr5.4:74234714-74234733 None:intergenic 40.0%
!! AGTCTAGGACTTTTTGTTGG+GGG + chr5.4:74234677-74234696 None:intergenic 40.0%
!! ATAAGCATGACGGATTCACT+GGG - chr5.4:74234858-74234877 MS.gene020685:intron 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG + chr5.4:74232447-74232466 None:intergenic 40.0%
!! GAGAATGTGGAATTTGTTGG+GGG + chr5.4:74234715-74234734 None:intergenic 40.0%
!! TGCCAAGTATTGACTAGGTT+TGG + chr5.4:74234577-74234596 None:intergenic 40.0%
!!! GATTGCAGTTTTAGATGCAG+CGG - chr5.4:74233487-74233506 MS.gene020685:CDS 40.0%
!!! GGGGGAAGTTGATTTTTTGT+TGG + chr5.4:74234658-74234677 None:intergenic 40.0%
AAAGCATGGTTACCGCTGAT+TGG - chr5.4:74232935-74232954 MS.gene020685:intron 45.0%
AATCAGCGGTAACCATGCTT+TGG + chr5.4:74232936-74232955 None:intergenic 45.0%
AATTCAATGGCAGGGTTGAG+TGG - chr5.4:74234884-74234903 MS.gene020685:intron 45.0%
ACAGATGAAGCGGGTAAAGA+AGG - chr5.4:74233449-74233468 MS.gene020685:CDS 45.0%
ACATAATTCGCGGTTCGTTC+CGG + chr5.4:74233225-74233244 None:intergenic 45.0%
ACCTTAAAAGCCGAGAATGG+AGG - chr5.4:74233273-74233292 MS.gene020685:CDS 45.0%
ACGATGCCATGAAGATTGGT+TGG - chr5.4:74233600-74233619 MS.gene020685:CDS 45.0%
AGACTCGTCCAAATCAAGCT+TGG + chr5.4:74232799-74232818 None:intergenic 45.0%
AGGAGGTTCTTGGTCAGTTA+CGG + chr5.4:74232649-74232668 None:intergenic 45.0%
ATGAAGCGGGTAAAGAAGGA+GGG - chr5.4:74233453-74233472 MS.gene020685:CDS 45.0%
CAGTTTGGCAAAGACCTCTT+CGG + chr5.4:74232616-74232635 None:intergenic 45.0%
CATGCTCCAAATGACCTTGT+GGG + chr5.4:74232491-74232510 None:intergenic 45.0%
CCTGACATAGAGCTAAACCT+TGG - chr5.4:74234914-74234933 MS.gene020685:CDS 45.0%
CGCTTCATCTGTAGTGAACT+TGG + chr5.4:74233441-74233460 None:intergenic 45.0%
CTACTTCCCACAAGGTCATT+TGG - chr5.4:74232482-74232501 MS.gene020685:intron 45.0%
CTTCTGTGGACCCCAAAATA+TGG - chr5.4:74232406-74232425 MS.gene020685:intron 45.0%
GAATGAGTTCACGAGACAGT+TGG - chr5.4:74231952-74231971 MS.gene020685:CDS 45.0%
GACTCGTCCAAATCAAGCTT+GGG + chr5.4:74232798-74232817 None:intergenic 45.0%
GATCTTGCCCAAGCTTGATT+TGG - chr5.4:74232788-74232807 MS.gene020685:intron 45.0%
GCAGGGAGTAATATTGGGTT+GGG + chr5.4:74232355-74232374 None:intergenic 45.0%
GCATTGCTATATTTCGCGAG+AGG + chr5.4:74232096-74232115 None:intergenic 45.0%
GGAATGAAGGAGGAAGTTGA+CGG + chr5.4:74234773-74234792 None:intergenic 45.0%
GGATTCTGTGGGATATGGTT+TGG + chr5.4:74234556-74234575 None:intergenic 45.0%
GGTTTGGATTCTGTGGGATA+TGG + chr5.4:74234531-74234550 None:intergenic 45.0%
GTGCCAAAATCAAACCCACT+TGG + chr5.4:74234374-74234393 None:intergenic 45.0%
GTGTATTATCCGACAGCTAG+TGG - chr5.4:74233542-74233561 MS.gene020685:CDS 45.0%
GTTGACGATGCCATGAAGAT+TGG - chr5.4:74233596-74233615 MS.gene020685:CDS 45.0%
TAACCATGCTTTGGTACGCT+TGG + chr5.4:74232927-74232946 None:intergenic 45.0%
TCCTCCTTCATTCCTATGCA+GGG - chr5.4:74234779-74234798 MS.gene020685:intron 45.0%
TCGAACATGAGATCTCAAGG+AGG - chr5.4:74234309-74234328 MS.gene020685:CDS 45.0%
TGCAGGGAGTAATATTGGGT+TGG + chr5.4:74232356-74232375 None:intergenic 45.0%
TTCCTCCTTCATTCCTATGC+AGG - chr5.4:74234778-74234797 MS.gene020685:intron 45.0%
! ACCTCCATTCTCGGCTTTTA+AGG + chr5.4:74233277-74233296 None:intergenic 45.0%
! AGTACCGAATTCGGGTACTT+CGG - chr5.4:74232875-74232894 MS.gene020685:intron 45.0%
! ATTCTCTCCGGTGCCAATTT+AGG + chr5.4:74234290-74234309 None:intergenic 45.0%
! CGTATAGGGGTATACAGCTT+CGG + chr5.4:74233151-74233170 None:intergenic 45.0%
! GGGATATGGTTTGGATGGTT+TGG + chr5.4:74234547-74234566 None:intergenic 45.0%
! TAGTGGTTGCCGTGATTTTG+TGG - chr5.4:74233559-74233578 MS.gene020685:CDS 45.0%
! TCTGTGGGATATGGTTTGGA+TGG + chr5.4:74234552-74234571 None:intergenic 45.0%
!! ACATCGTCGACTTCTTGACT+GGG + chr5.4:74232684-74232703 None:intergenic 45.0%
!! CAACCTGCCAAGTATTGACT+AGG + chr5.4:74234582-74234601 None:intergenic 45.0%
!! CCAAGGTTTAGCTCTATGTC+AGG + chr5.4:74234917-74234936 None:intergenic 45.0%
!! GATAAGCATGACGGATTCAC+TGG - chr5.4:74234857-74234876 MS.gene020685:intron 45.0%
!! GTCTAGGACTTTTTGTTGGG+GGG + chr5.4:74234676-74234695 None:intergenic 45.0%
!! TACATCGTCGACTTCTTGAC+TGG + chr5.4:74232685-74232704 None:intergenic 45.0%
!! TCTTTGGAATCTTGACGTCG+GGG + chr5.4:74232446-74232465 None:intergenic 45.0%
!! TGGTACGTGAGTACCGAATT+CGG - chr5.4:74232866-74232885 MS.gene020685:intron 45.0%
!! TTGCCTAGTGGTATTGGCTT+GGG + chr5.4:74234350-74234369 None:intergenic 45.0%
!!! AGTTTTAGATGCAGCGGAAC+TGG - chr5.4:74233493-74233512 MS.gene020685:CDS 45.0%
!!! CATATTTTGGGGTCCACAGA+AGG + chr5.4:74232408-74232427 None:intergenic 45.0%
!!! CCATTCTCGGCTTTTAAGGT+CGG + chr5.4:74233273-74233292 None:intergenic 45.0%
AACGAAAAGGCAGAGCCTAC+CGG + chr5.4:74232037-74232056 None:intergenic 50.0%
ACCTCAAGCATGCGCCAATT+AGG + chr5.4:74233743-74233762 None:intergenic 50.0%
ACTTCTTGACTGGGAACCAC+AGG + chr5.4:74232675-74232694 None:intergenic 50.0%
ATACCACTAGGCAACCCAAG+TGG - chr5.4:74234356-74234375 MS.gene020685:CDS 50.0%
ATGAAGGAGGAAGTTGACGG+TGG + chr5.4:74234770-74234789 None:intergenic 50.0%
ATGGTGATTGTGGCAGCGTT+AGG + chr5.4:74232525-74232544 None:intergenic 50.0%
ATTCGGGTACTTCGGCGTAT+TGG - chr5.4:74232883-74232902 MS.gene020685:intron 50.0%
CCGACCTTAAAAGCCGAGAA+TGG - chr5.4:74233270-74233289 MS.gene020685:CDS 50.0%
CCTCAAGCATGCGCCAATTA+GGG + chr5.4:74233742-74233761 None:intergenic 50.0%
CGATGCATGGAATGGATGGA+CGG + chr5.4:74232560-74232579 None:intergenic 50.0%
CTAAACCTTGGCAGTCCTAG+AGG - chr5.4:74234926-74234945 MS.gene020685:CDS 50.0%
CTCACTCAGTCCTCAACTCA+TGG + chr5.4:74233006-74233025 None:intergenic 50.0%
CTTGGTCAGTTACGGGAGTT+AGG + chr5.4:74232641-74232660 None:intergenic 50.0%
GAACCAAGCGTACCAAAGCA+TGG - chr5.4:74232921-74232940 MS.gene020685:intron 50.0%
GACGGTGGAGGCTGTAATTT+AGG + chr5.4:74234755-74234774 None:intergenic 50.0%
GACTCCCTGCATAGGAATGA+AGG + chr5.4:74234786-74234805 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + chr5.4:74232564-74232583 None:intergenic 50.0%
GATCCCAAGCCAATACCACT+AGG - chr5.4:74234344-74234363 MS.gene020685:CDS 50.0%
GATGAAGCGGGTAAAGAAGG+AGG - chr5.4:74233452-74233471 MS.gene020685:CDS 50.0%
GCATGCTCCAAATGACCTTG+TGG + chr5.4:74232492-74232511 None:intergenic 50.0%
GGAGGTTCTTGGTCAGTTAC+GGG + chr5.4:74232648-74232667 None:intergenic 50.0%
TACCACTAGGCAACCCAAGT+GGG - chr5.4:74234357-74234376 MS.gene020685:CDS 50.0%
TACCGGAATACACGGCGTAA+CGG + chr5.4:74232020-74232039 None:intergenic 50.0%
TACGGGAGTTAGGAGCAGTT+TGG + chr5.4:74232631-74232650 None:intergenic 50.0%
TAGCCCACCTAAATTGGCAC+CGG - chr5.4:74234280-74234299 MS.gene020685:CDS 50.0%
TATACAGCTTCGGTACGCTC+CGG + chr5.4:74233141-74233160 None:intergenic 50.0%
TCATTCCTATGCAGGGAGTC+AGG - chr5.4:74234786-74234805 MS.gene020685:intron 50.0%
TCCCTGCATAGGAATGAAGG+AGG + chr5.4:74234783-74234802 None:intergenic 50.0%
TGGTGATTGTGGCAGCGTTA+GGG + chr5.4:74232524-74232543 None:intergenic 50.0%
TGTTGATCCTCACACCGAAG+AGG - chr5.4:74232599-74232618 MS.gene020685:intron 50.0%
TGTTGGGGGGAAATTGATGG+TGG + chr5.4:74234641-74234660 None:intergenic 50.0%
TTTAGATGCAGCGGAACTGG+CGG - chr5.4:74233496-74233515 MS.gene020685:CDS 50.0%
! AGACATGGTGGAGCTATTGC+AGG + chr5.4:74232373-74232392 None:intergenic 50.0%
! CTTGGGTTGCCTAGTGGTAT+TGG + chr5.4:74234356-74234375 None:intergenic 50.0%
! GACATGGTGGAGCTATTGCA+GGG + chr5.4:74232372-74232391 None:intergenic 50.0%
! TACGCCGAAGTACCCGAATT+CGG + chr5.4:74232882-74232901 None:intergenic 50.0%
! TCTCCGGTGCCAATTTAGGT+GGG + chr5.4:74234286-74234305 None:intergenic 50.0%
! TGGTTGCCGTGATTTTGTGG+TGG - chr5.4:74233562-74233581 MS.gene020685:CDS 50.0%
!! AGTGGTATTGGCTTGGGATC+TGG + chr5.4:74234344-74234363 None:intergenic 50.0%
!! GGTACGTGAGTACCGAATTC+GGG - chr5.4:74232867-74232886 MS.gene020685:intron 50.0%
!! GTGGTATTGGCTTGGGATCT+GGG + chr5.4:74234343-74234362 None:intergenic 50.0%
!! GTTGCCTAGTGGTATTGGCT+TGG + chr5.4:74234351-74234370 None:intergenic 50.0%
!! TCACTTGGTGCTTGGCTCAT+TGG + chr5.4:74234984-74235003 None:intergenic 50.0%
!!! ATTTTGGGGTCCACAGAAGG+TGG + chr5.4:74232405-74232424 None:intergenic 50.0%
AAGGAGGAAGTTGACGGTGG+AGG + chr5.4:74234767-74234786 None:intergenic 55.0%
AGGTGGCTGAGGAAGAGACA+TGG + chr5.4:74232388-74232407 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTG+AGG - chr5.4:74233739-74233758 MS.gene020685:intron 55.0%
GCAGAGCCTACCGGAATACA+CGG + chr5.4:74232028-74232047 None:intergenic 55.0%
GGCAGAGACGATGCATGGAA+TGG + chr5.4:74232568-74232587 None:intergenic 55.0%
GGTGATTGTGGCAGCGTTAG+GGG + chr5.4:74232523-74232542 None:intergenic 55.0%
GTTACGCCGTGTATTCCGGT+AGG - chr5.4:74232019-74232038 MS.gene020685:CDS 55.0%
TCAACGGCAGAGACGATGCA+TGG + chr5.4:74232573-74232592 None:intergenic 55.0%
TCACGGCAACCACTAGCTGT+CGG + chr5.4:74233554-74233573 None:intergenic 55.0%
TCATGCCTGACTCCCTGCAT+AGG + chr5.4:74234794-74234813 None:intergenic 55.0%
TCTTCCTCAGCCACCTTCTG+TGG - chr5.4:74232392-74232411 MS.gene020685:CDS 55.0%
TCTTGACTGGGAACCACAGG+AGG + chr5.4:74232672-74232691 None:intergenic 55.0%
TGGCTGAGGAAGAGACATGG+TGG + chr5.4:74232385-74232404 None:intergenic 55.0%
! AACCCACTTGGGTTGCCTAG+TGG + chr5.4:74234362-74234381 None:intergenic 55.0%
! ATGGTGGAGGAGGAAGTCGA+AGG + chr5.4:74234625-74234644 None:intergenic 55.0%
! CTCTCCGGTGCCAATTTAGG+TGG + chr5.4:74234287-74234306 None:intergenic 55.0%
! CTTTGGTACGCTTGGTTCGC+TGG + chr5.4:74232919-74232938 None:intergenic 55.0%
! GCAAAGACCTCTTCGGTGTG+AGG + chr5.4:74232609-74232628 None:intergenic 55.0%
!! AGGCTAGCTTTGTGCCCTGT+AGG - chr5.4:74233928-74233947 MS.gene020685:intron 55.0%
!! GGGGAAATTGATGGTGGAGG+AGG + chr5.4:74234635-74234654 None:intergenic 55.0%
!! TGGGGGGAAATTGATGGTGG+AGG + chr5.4:74234638-74234657 None:intergenic 55.0%
CCAAGAACCTCCTCCTCCTG+TGG - chr5.4:74232656-74232675 MS.gene020685:intron 60.0%
CCACAGGAGGAGGAGGTTCT+TGG + chr5.4:74232659-74232678 None:intergenic 60.0%
CGCCGTTACGCCGTGTATTC+CGG - chr5.4:74232015-74232034 MS.gene020685:CDS 60.0%
CGGAATACACGGCGTAACGG+CGG + chr5.4:74232017-74232036 None:intergenic 60.0%
GGCAACGAGTCAGCACCTCT+AGG + chr5.4:74234944-74234963 None:intergenic 60.0%
GTTCGTTCCGGTACGCGGTA+CGG + chr5.4:74233213-74233232 None:intergenic 60.0%
TACACGGCGTAACGGCGGAA+TGG + chr5.4:74232012-74232031 None:intergenic 60.0%
TGACTGGGAACCACAGGAGG+AGG + chr5.4:74232669-74232688 None:intergenic 60.0%
TGGAATCTTGACGTCGGGGC+CGG + chr5.4:74232442-74232461 None:intergenic 60.0%
TTCGTTCCGGTACGCGGTAC+GGG + chr5.4:74233212-74233231 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+AGG + chr5.4:74234619-74234638 None:intergenic 60.0%
!! CAGCACCTCTAGGACTGCCA+AGG + chr5.4:74234934-74234953 None:intergenic 60.0%
CTGGGAACCACAGGAGGAGG+AGG + chr5.4:74232666-74232685 None:intergenic 65.0%
GGGTCCACAGAAGGTGGCTG+AGG + chr5.4:74232399-74232418 None:intergenic 65.0%
TCCGGTACGCGGTACGGGTA+CGG + chr5.4:74233207-74233226 None:intergenic 65.0%
TCGCGGTTCGTTCCGGTACG+CGG + chr5.4:74233218-74233237 None:intergenic 65.0%
ACGGGTTCGCGGTACGCGAC+AGG + chr5.4:74233188-74233207 None:intergenic 70.0%
CCCGTACCCGTACCGCGTAC+CGG - chr5.4:74233203-74233222 MS.gene020685:CDS 70.0%
CCGGTACGCGGTACGGGTAC+GGG + chr5.4:74233206-74233225 None:intergenic 70.0%
GCGGTACGGGTACGGGTTCG+CGG + chr5.4:74233199-74233218 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 74231923 74235003 74231923 ID=MS.gene020685
chr5.4 mRNA 74231923 74235003 74231923 ID=MS.gene020685.t1;Parent=MS.gene020685
chr5.4 exon 74234890 74235003 74234890 ID=MS.gene020685.t1.exon1;Parent=MS.gene020685.t1
chr5.4 CDS 74234890 74235003 74234890 ID=cds.MS.gene020685.t1;Parent=MS.gene020685.t1
chr5.4 exon 74234225 74234494 74234225 ID=MS.gene020685.t1.exon2;Parent=MS.gene020685.t1
chr5.4 CDS 74234225 74234494 74234225 ID=cds.MS.gene020685.t1;Parent=MS.gene020685.t1
chr5.4 exon 74233166 74233669 74233166 ID=MS.gene020685.t1.exon3;Parent=MS.gene020685.t1
chr5.4 CDS 74233166 74233669 74233166 ID=cds.MS.gene020685.t1;Parent=MS.gene020685.t1
chr5.4 exon 74232329 74232415 74232329 ID=MS.gene020685.t1.exon4;Parent=MS.gene020685.t1
chr5.4 CDS 74232329 74232415 74232329 ID=cds.MS.gene020685.t1;Parent=MS.gene020685.t1
chr5.4 exon 74231923 74232162 74231923 ID=MS.gene020685.t1.exon5;Parent=MS.gene020685.t1
chr5.4 CDS 74231923 74232162 74231923 ID=cds.MS.gene020685.t1;Parent=MS.gene020685.t1
Gene Sequence

>MS.gene020685

ATGCTACGTATAGCGAAGAAATGCACGGCGAATGAGTTCACGAGACAGTTGGCACATTTTAATGGCTTTGGCTTCTGTGTTTTTCGCCATTCCGCCGTTACGCCGTGTATTCCGGTAGGCTCTGCCTTTTCGTTTGCTAAGCTTGTATTCATGATGCTCTCATGAGGATTCCTCTCGCGAAATATAGCAATGCTCTATCATTAAAGAAGTTCAAATATGTCAATTTAAACATGCTTATGATACACATTTATAAGCTTAAGATTACTTTCATACATGATAACTAACAAACTCATGTTAACTTAAAAACTTGGCCAAGATTACCTCAATTATAAGCTTAATCCATGCAATCATCTATAACTGAAACACTACAAATAAACTTAAAACATTTTGTAACTAACTACTTCTCGCTCTTTTTTTCACTATCATACACCCAACCCAATATTACTCCCTGCAATAGCTCCACCATGTCTCTTCCTCAGCCACCTTCTGTGGACCCCAAAATATGGTAGAAATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACAAGGTCATTTGGAGCATGCATGTTCTTCCCCTAACGCTGCCACAATCACCATTCTTGATAGTTACCGTCCATCCATTCCATGCATCGTCTCTGCCGTTGATCTCTTTGTTGATCCTCACACCGAAGAGGTCTTTGCCAAACTGCTCCTAACTCCCGTAACTGACCAAGAACCTCCTCCTCCTGTGGTTCCCAGTCAAGAAGTCGACGATGTAAACAATCTTGTTTCCTATGTCAAGACTCTCACACAATCAGATTGTACAAGAGTATTACATGTACATCGTGAATGCTCTGACTTGATCTTGCCCAAGCTTGATTTGGACGAGTCTCAGAGCCATAGTCACATAATTCTTGTAATCAAGGGAGAATTGCGAATTGGTACGTGAGTACCGAATTCGGGTACTTCGGCGTATTGGTACGCACAAACCAGCGAACCAAGCGTACCAAAGCATGGTTACCGCTGATTGGTTGAAAAAAAAGGTTTAGACTACAAAGTAAATATAAAATGAATCACCATGAGTTGAGGACTGAGTGAGAAGAACATGAACAAAGAGTGGAGAATGAGAAACTTGGTAGAAGAAATTAGGTAAAAAAATACTTTTTTTTTATAGCTCACTATATTTATTTAGGTATTATTACTACTACGAACCGGAGCGTACCGAAGCTGTATACCCCTATACGTACCAAATTCTTATCCTGTCGCGTACCGCGAACCCGTACCCGTACCGCGTACCGGAACGAACCGCGAATTATGTGACTATGCTCAGAGCATCACAGTCACCGACCTTAAAAGCCGAGAATGGAGGTACACTTATACCTACGCGAAACTCAGTAGATTACATACTGGCTGGTCTTACTTTGTACAAGAGAAGAAACTCGTCACCAACGATTCTGTTGTTTTCATTAAAAACTCTGCTGGAAAGATATTTGTCGGAATTCGCAGGAATACCAAGTTCACTACAGATGAAGCGGGTAAAGAAGGAGGGAATTTAACTGAGATTGCAGTTTTAGATGCAGCGGAACTGGCGGAGAAGAATACAGTGTTTGAAGTTGTGTATTATCCGACAGCTAGTGGTTGCCGTGATTTTGTGGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAGCTTCCTTTGAAGAAAGATGAATCTTCAAATTCAAAGATGACATATTCTCAGCCTAAAGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTACTTATGCTATCTTATTTTGGTCTTGTTATATTTCAAGTGCATATTCTTATCAAGTTTATAGTTTTTACCAAATTTGAATCCATAATAAGATTATTTGATTGATAAATATGAGTGTTGAAGTTAATACATGATTCATTTAGATATCTAAAATTATGAAACTTAGTAGGCTAGCTTTGTGCCCTGTAGGATAAGTTCCAAATGTAATTTGAAAATTAAGAGACAGAAAATTGTTACAGAATCTCCATCTCTAACATTAATAATTCCCTTGTTCTTGCACTTGATAGATATCTCATGACACTTAATGTATACTTTATGTAATCAGATTACATTTTAGAATCGATTGTTTGTATCACACCCGTTTTTATATGTAACTCCCTACTTGACTTGCTGCAATAGTGATATTCACTAAAAAATAGTTATGAAGTTTAAGAACCTTCTAAGCATTTCTCATTGTAAATACTCCTAATGCTAAGTAGAATCTTGATTTTGGCACATGTTTTTTTGAAGAAGCTAAATTAGCCCACCTAAATTGGCACCGGAGAGAATCGAACATGAGATCTCAAGGAGGAGCACACTCCCAGATCCCAAGCCAATACCACTAGGCAACCCAAGTGGGTTTGATTTTGGCACATGTTATTAATCGATAAGAAACTTTTGATGTCTAAATACATATATACTATATATAATTTATCAAATATTTCAAATGATTGATATTGATTCTTACAATTTTTTATCTCTTTATCATAGGTTAACTGGGATGGACCTAATATCCCACAGAATCCAAACCATCCAAACCATATCCCACAGAATCCAAACCTAGTCAATACTTGGCAGGTTGAAGTTTACAACATACCTGCACCTTCGACTTCCTCCTCCACCATCAATTTCCCCCCAACAAAAAATCAACTTCCCCCCAACAAAAAGTCCTAGACTTTCTCAGAGTTCTTCCCCCCAACAAATTCCACATTCTCTAATGCCAATTACACTGCCTAAATTACAGCCTCCACCGTCAACTTCCTCCTTCATTCCTATGCAGGGAGTCAGGCATGAGCATGACCATTTATCTGTATCAGATTCATCAAAAGTTTTGAATGATAAGCATGACGGATTCACTGGGAATAATTCAATGGCAGGGTTGAGTGGTACTTTGCCTGACATAGAGCTAAACCTTGGCAGTCCTAGAGGTGCTGACTCGTTGCCTCATAACCATAATAGGTCCAATGAGCCAAGCACCAAGTGA
Protein sequence

>MS.gene020685.t1

MLRIAKKCTANEFTRQLAHFNGFGFCVFRHSAVTPCIPKCAGPDVKIPKIHSKVYYFPQGHLEHACSSPNAATITILDSYRPSIPCIVSAVDLFVDPHTEEVFAKLLLTPVTDQEPPPPVVPSQEVDDSITVTDLKSREWRYTYTYAKLSRLHTGWSYFVQEKKLVTNDSVVFIKNSAGKIFVGIRRNTKFTTDEAGKEGGNLTEIAVLDAAELAEKNTVFEVVYYPTASGCRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKDESSNSKMTYSQPKGTISFVFNDSSNVPNWRMLEVNWDGPNIPQNPNHPNHIPQNPNLVNTWQPPPSTSSFIPMQGVRHEHDHLSVSDSSKVLNDKHDGFTGNNSMAGLSGTLPDIELNLGSPRGADSLPHNHNRSNEPSTK