Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020712.t1 | XP_003617710.2 | 90.9 | 504 | 40 | 2 | 1 | 499 | 1 | 503 | 3.50E-127 | 465.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020712.t1 | Q9FM80 | 70.3 | 475 | 111 | 3 | 33 | 498 | 42 | 495 | 1.1e-68 | 262.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020712.t1 | G7K4F3 | 90.9 | 504 | 40 | 2 | 1 | 499 | 1 | 503 | 2.6e-127 | 465.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020712.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020712.t1 | MTR_5g094610 | 96.047 | 506 | 10 | 3 | 1 | 499 | 1 | 503 | 0.0 | 975 |
| MS.gene020712.t1 | MTR_2g034600 | 32.456 | 228 | 139 | 7 | 226 | 447 | 215 | 433 | 3.27e-26 | 112 |
| MS.gene020712.t1 | MTR_2g034600 | 30.256 | 195 | 125 | 5 | 255 | 445 | 208 | 395 | 2.96e-17 | 84.7 |
| MS.gene020712.t1 | MTR_2g034600 | 27.111 | 225 | 135 | 6 | 181 | 399 | 277 | 478 | 7.85e-17 | 83.6 |
| MS.gene020712.t1 | MTR_2g034600 | 32.456 | 228 | 139 | 7 | 226 | 447 | 276 | 494 | 3.87e-26 | 112 |
| MS.gene020712.t1 | MTR_2g034600 | 30.102 | 196 | 126 | 5 | 254 | 445 | 268 | 456 | 3.38e-17 | 84.7 |
| MS.gene020712.t1 | MTR_2g034600 | 27.273 | 231 | 138 | 7 | 175 | 399 | 333 | 539 | 7.39e-17 | 84.0 |
| MS.gene020712.t1 | MTR_2g034600 | 32.456 | 228 | 139 | 7 | 226 | 447 | 280 | 498 | 4.13e-26 | 112 |
| MS.gene020712.t1 | MTR_2g034600 | 30.256 | 195 | 125 | 5 | 255 | 445 | 273 | 460 | 3.29e-17 | 84.7 |
| MS.gene020712.t1 | MTR_2g034600 | 27.273 | 231 | 138 | 7 | 175 | 399 | 337 | 543 | 8.29e-17 | 83.6 |
| MS.gene020712.t1 | MTR_2g067230 | 27.459 | 244 | 159 | 6 | 223 | 465 | 70 | 296 | 1.76e-25 | 107 |
| MS.gene020712.t1 | MTR_5g068860 | 24.924 | 329 | 202 | 7 | 187 | 477 | 171 | 492 | 9.28e-25 | 107 |
| MS.gene020712.t1 | MTR_5g068860 | 25.263 | 190 | 136 | 3 | 217 | 406 | 134 | 317 | 5.98e-11 | 65.1 |
| MS.gene020712.t1 | MTR_4g084630 | 28.195 | 266 | 177 | 6 | 184 | 444 | 42 | 298 | 1.06e-23 | 102 |
| MS.gene020712.t1 | MTR_4g084630 | 27.753 | 227 | 160 | 2 | 222 | 445 | 41 | 266 | 1.13e-18 | 87.4 |
| MS.gene020712.t1 | MTR_5g041630 | 29.319 | 191 | 132 | 1 | 257 | 447 | 88 | 275 | 1.11e-20 | 93.2 |
| MS.gene020712.t1 | MTR_6g003960 | 28.829 | 222 | 154 | 3 | 223 | 444 | 350 | 567 | 3.62e-20 | 94.4 |
| MS.gene020712.t1 | MTR_4g070060 | 23.630 | 292 | 185 | 5 | 190 | 445 | 277 | 566 | 2.53e-19 | 91.7 |
| MS.gene020712.t1 | MTR_4g070060 | 24.476 | 286 | 198 | 7 | 172 | 446 | 184 | 462 | 2.53e-12 | 69.7 |
| MS.gene020712.t1 | MTR_1g115415 | 26.484 | 219 | 154 | 4 | 226 | 442 | 215 | 428 | 1.17e-17 | 85.9 |
| MS.gene020712.t1 | MTR_1g115415 | 24.255 | 235 | 168 | 6 | 223 | 453 | 108 | 336 | 1.68e-14 | 76.3 |
| MS.gene020712.t1 | MTR_2g049780 | 24.215 | 223 | 154 | 4 | 223 | 441 | 70 | 281 | 3.63e-16 | 79.7 |
| MS.gene020712.t1 | MTR_7g081270 | 25.984 | 254 | 173 | 7 | 221 | 471 | 42 | 283 | 7.58e-16 | 78.2 |
| MS.gene020712.t1 | MTR_8g105560 | 25.789 | 190 | 137 | 4 | 223 | 410 | 86 | 273 | 1.98e-13 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020712.t1 | AT5G55580 | 63.158 | 475 | 145 | 3 | 33 | 498 | 42 | 495 | 0.0 | 572 |
| MS.gene020712.t1 | AT5G55580 | 73.669 | 357 | 90 | 1 | 142 | 498 | 37 | 389 | 0.0 | 552 |
| MS.gene020712.t1 | AT5G55580 | 58.824 | 374 | 124 | 3 | 33 | 397 | 42 | 394 | 1.70e-138 | 405 |
| MS.gene020712.t1 | AT2G21710 | 26.502 | 283 | 198 | 5 | 191 | 470 | 320 | 595 | 7.05e-27 | 114 |
| MS.gene020712.t1 | AT2G21710 | 23.975 | 317 | 221 | 8 | 172 | 477 | 191 | 498 | 2.43e-13 | 72.8 |
| MS.gene020712.t1 | AT4G02990 | 23.952 | 334 | 207 | 6 | 183 | 477 | 175 | 500 | 8.87e-24 | 105 |
| MS.gene020712.t1 | AT4G02990 | 23.952 | 334 | 207 | 6 | 183 | 477 | 175 | 500 | 8.87e-24 | 105 |
| MS.gene020712.t1 | AT1G78930 | 28.111 | 217 | 152 | 3 | 228 | 444 | 342 | 554 | 7.13e-23 | 102 |
| MS.gene020712.t1 | AT1G78930 | 27.879 | 165 | 111 | 3 | 216 | 377 | 397 | 556 | 3.91e-14 | 75.5 |
| MS.gene020712.t1 | AT4G38160 | 26.692 | 266 | 181 | 6 | 185 | 444 | 43 | 300 | 9.60e-23 | 99.8 |
| MS.gene020712.t1 | AT4G38160 | 29.310 | 232 | 156 | 6 | 222 | 447 | 41 | 270 | 1.32e-20 | 93.2 |
| MS.gene020712.t1 | AT4G38160 | 26.692 | 266 | 181 | 6 | 185 | 444 | 43 | 300 | 9.60e-23 | 99.8 |
| MS.gene020712.t1 | AT4G38160 | 29.310 | 232 | 156 | 6 | 222 | 447 | 41 | 270 | 1.32e-20 | 93.2 |
| MS.gene020712.t1 | AT4G38160 | 26.692 | 266 | 181 | 6 | 185 | 444 | 43 | 300 | 1.32e-22 | 99.8 |
| MS.gene020712.t1 | AT4G38160 | 29.310 | 232 | 156 | 6 | 222 | 447 | 41 | 270 | 1.66e-20 | 93.6 |
| MS.gene020712.t1 | AT4G38160 | 26.692 | 266 | 181 | 6 | 185 | 444 | 43 | 300 | 3.21e-22 | 98.6 |
| MS.gene020712.t1 | AT4G38160 | 29.310 | 232 | 156 | 6 | 222 | 447 | 41 | 270 | 3.28e-20 | 92.8 |
| MS.gene020712.t1 | AT2G03050 | 26.786 | 224 | 154 | 5 | 223 | 444 | 33 | 248 | 6.90e-21 | 93.2 |
| MS.gene020712.t1 | AT2G03050 | 26.786 | 224 | 154 | 5 | 223 | 444 | 34 | 249 | 8.42e-21 | 92.8 |
| MS.gene020712.t1 | AT2G44020 | 25.763 | 295 | 181 | 5 | 188 | 446 | 145 | 437 | 2.08e-20 | 94.7 |
| MS.gene020712.t1 | AT2G44020 | 25.957 | 235 | 152 | 8 | 216 | 447 | 83 | 298 | 1.94e-11 | 66.6 |
| MS.gene020712.t1 | AT2G21710 | 24.896 | 241 | 174 | 4 | 191 | 428 | 320 | 556 | 2.14e-20 | 94.7 |
| MS.gene020712.t1 | AT2G21710 | 23.975 | 317 | 221 | 8 | 172 | 477 | 191 | 498 | 7.95e-13 | 71.2 |
| MS.gene020712.t1 | AT2G21710 | 24.686 | 239 | 173 | 4 | 191 | 426 | 320 | 554 | 6.01e-20 | 93.6 |
| MS.gene020712.t1 | AT2G21710 | 23.975 | 317 | 221 | 8 | 172 | 477 | 191 | 498 | 4.66e-13 | 72.0 |
| MS.gene020712.t1 | AT2G34620 | 26.104 | 249 | 166 | 6 | 223 | 467 | 56 | 290 | 1.37e-19 | 89.7 |
| MS.gene020712.t1 | AT4G14605 | 26.027 | 219 | 155 | 5 | 226 | 444 | 236 | 447 | 1.79e-19 | 91.7 |
| MS.gene020712.t1 | AT4G14605 | 25.778 | 225 | 138 | 6 | 181 | 399 | 262 | 463 | 1.18e-13 | 73.6 |
| MS.gene020712.t1 | AT4G14605 | 26.027 | 219 | 155 | 5 | 226 | 444 | 236 | 447 | 1.79e-19 | 91.7 |
| MS.gene020712.t1 | AT4G14605 | 25.778 | 225 | 138 | 6 | 181 | 399 | 262 | 463 | 1.18e-13 | 73.6 |
| MS.gene020712.t1 | AT2G36000 | 27.600 | 250 | 157 | 8 | 223 | 466 | 74 | 305 | 4.76e-19 | 88.6 |
| MS.gene020712.t1 | AT2G36000 | 27.600 | 250 | 157 | 8 | 223 | 466 | 74 | 305 | 5.06e-19 | 88.6 |
| MS.gene020712.t1 | AT3G18870 | 25.984 | 254 | 130 | 8 | 226 | 471 | 69 | 272 | 1.24e-16 | 80.5 |
Find 83 sgRNAs with CRISPR-Local
Find 166 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAAGCACCTTTGGAATTA+AGG | 0.140140 | 5.4:+74439871 | MS.gene020712:CDS |
| AGCAATAATTTGCCCTATTT+TGG | 0.169836 | 5.4:-74440110 | None:intergenic |
| GACTCACGTTGCTTTCTTAA+TGG | 0.188627 | 5.4:+74440069 | MS.gene020712:CDS |
| CATCTTCACAATGCTCTCTT+TGG | 0.219102 | 5.4:-74440326 | None:intergenic |
| TGACTTCAATCTCTTGTCTT+TGG | 0.225373 | 5.4:-74439004 | None:intergenic |
| CGTTGCTTTCTTAATGGGCT+TGG | 0.237557 | 5.4:+74440075 | MS.gene020712:CDS |
| GGATGCTATTCTCTGACTTT+TGG | 0.242258 | 5.4:-74439372 | None:intergenic |
| ACATGTGCCTCTCATAAATT+TGG | 0.254970 | 5.4:-74439907 | None:intergenic |
| CTCCTTAGGAAATTTATTCT+TGG | 0.307382 | 5.4:-74440400 | None:intergenic |
| TCCAACGTATTGACATTTCA+TGG | 0.312784 | 5.4:+74440263 | MS.gene020712:CDS |
| ATCTTCACAATGCTCTCTTT+GGG | 0.327138 | 5.4:-74440325 | None:intergenic |
| GCTAAGGTACATCGGGTATT+TGG | 0.329609 | 5.4:-74440721 | None:intergenic |
| CCAAGAGGAAATGGTCCTTT+TGG | 0.331786 | 5.4:-74440804 | None:intergenic |
| CTTGATTACTTAAGCACCTT+TGG | 0.335021 | 5.4:+74439863 | MS.gene020712:CDS |
| TCGTCCTCGTCATAAGTTCT+TGG | 0.344950 | 5.4:+74440764 | MS.gene020712:CDS |
| ACTCACGTTGCTTTCTTAAT+GGG | 0.345489 | 5.4:+74440070 | MS.gene020712:CDS |
| TAGATTACTTAATGAGTCTT+GGG | 0.347132 | 5.4:+74439969 | MS.gene020712:CDS |
| GATGTACCTTAGCTTGTCTT+TGG | 0.348758 | 5.4:+74440731 | MS.gene020712:CDS |
| GGCGTACCTAGTACCAAAAT+AGG | 0.359195 | 5.4:+74440097 | MS.gene020712:CDS |
| GTTGCTTTCTTAATGGGCTT+GGG | 0.365837 | 5.4:+74440076 | MS.gene020712:CDS |
| GTTGTCTGAAGAGATTCTTT+TGG | 0.368169 | 5.4:+74439820 | MS.gene020712:CDS |
| GCATGATTAGAGGAAGGAAT+TGG | 0.374741 | 5.4:-74438378 | None:intergenic |
| TTGAAGTCAAAGACAGTTGC+AGG | 0.386479 | 5.4:+74439018 | MS.gene020712:CDS |
| AGAAGCAGAAGCATGATTAG+AGG | 0.407915 | 5.4:-74438388 | None:intergenic |
| TGATGATGAAGATGAAGAAA+TGG | 0.419929 | 5.4:+74438524 | MS.gene020712:CDS |
| GAAGTTGTGGTTGCCTCAAA+AGG | 0.421347 | 5.4:-74440018 | None:intergenic |
| CTGCATTACAGTATTGATGA+TGG | 0.426933 | 5.4:+74440370 | MS.gene020712:CDS |
| ACTAAAGATCCAAGAGGAAA+TGG | 0.436568 | 5.4:-74440813 | None:intergenic |
| AGATATTTAATTGAGGAAGT+TGG | 0.438021 | 5.4:+74440187 | MS.gene020712:CDS |
| ATAGATTACTTAATGAGTCT+TGG | 0.440723 | 5.4:+74439968 | MS.gene020712:CDS |
| AGAATGTTGAAAGGATTGTA+AGG | 0.447642 | 5.4:-74438354 | None:intergenic |
| TGACAAAATGATTCATCAGT+TGG | 0.450331 | 5.4:-74440837 | None:intergenic |
| GCAGGTGCTATCTCTGTCCT+TGG | 0.454121 | 5.4:+74440632 | MS.gene020712:intron |
| CAAAGAGAGCATTGTGAAGA+TGG | 0.456446 | 5.4:+74440327 | MS.gene020712:CDS |
| TAGACAACAAGAAAAGGAAA+AGG | 0.464265 | 5.4:+74439310 | MS.gene020712:CDS |
| CCAAAAGGACCATTTCCTCT+TGG | 0.467379 | 5.4:+74440804 | MS.gene020712:CDS |
| TTCGATGGAGAAAAGATATC+CGG | 0.473213 | 5.4:+74439796 | MS.gene020712:CDS |
| TATGACGAGGACGAATTCTC+TGG | 0.474277 | 5.4:-74440755 | None:intergenic |
| AATTGAGGAAGTTGGCATCA+AGG | 0.486070 | 5.4:+74440195 | MS.gene020712:CDS |
| TCAATTAAATATCTTACTGT+TGG | 0.487640 | 5.4:-74440178 | None:intergenic |
| GATGTTTCTTAACAAAGAGT+TGG | 0.488384 | 5.4:+74440297 | MS.gene020712:CDS |
| TACAGATAGACAACAAGAAA+AGG | 0.490910 | 5.4:+74439304 | MS.gene020712:intron |
| TTCCAAGAATAAATTTCCTA+AGG | 0.495242 | 5.4:+74440398 | MS.gene020712:CDS |
| AATTAATGTATGTTCTGCAC+AGG | 0.499032 | 5.4:+74439940 | MS.gene020712:CDS |
| AAAGCCGAAGTATTTGTACT+TGG | 0.508230 | 5.4:+74440665 | MS.gene020712:CDS |
| GCGTACCTAGTACCAAAATA+GGG | 0.510052 | 5.4:+74440098 | MS.gene020712:CDS |
| AACAGTAAGATATTTAATTG+AGG | 0.516231 | 5.4:+74440180 | MS.gene020712:CDS |
| TTGTGATTCCTTAATTCCAA+AGG | 0.518718 | 5.4:-74439879 | None:intergenic |
| ACCAATCGAAAATCACCCTA+TGG | 0.522397 | 5.4:+74438477 | MS.gene020712:CDS |
| TCTTTGGCATTAAGATCAGT+AGG | 0.525087 | 5.4:-74438988 | None:intergenic |
| CGGCTTTAGATTAGCTTCCA+AGG | 0.526666 | 5.4:-74440649 | None:intergenic |
| ATAAATTTCCTAAGGAGCAT+AGG | 0.526985 | 5.4:+74440406 | MS.gene020712:CDS |
| AAGACAAGCTAAGGTACATC+GGG | 0.535136 | 5.4:-74440728 | None:intergenic |
| GTAGTTTCGTAGAAATTCGA+TGG | 0.537172 | 5.4:+74439781 | MS.gene020712:intron |
| TTTACCAAGTACAAATACTT+CGG | 0.538689 | 5.4:-74440669 | None:intergenic |
| GTACATCGGGTATTTGGTCA+AGG | 0.541793 | 5.4:-74440715 | None:intergenic |
| TCTCTAAAGAAAGCTCCAAA+AGG | 0.542731 | 5.4:+74440789 | MS.gene020712:CDS |
| GGAATTGGAAGAATGTTGAA+AGG | 0.542797 | 5.4:-74438363 | None:intergenic |
| TCTGGTCCAAAGACAAGCTA+AGG | 0.551617 | 5.4:-74440737 | None:intergenic |
| AGACAACAAGAAAAGGAAAA+GGG | 0.551962 | 5.4:+74439311 | MS.gene020712:CDS |
| ATACGTTGGACAAGAATTTG+AGG | 0.570242 | 5.4:-74440250 | None:intergenic |
| ATGTATGTTCTGCACAGGAA+AGG | 0.591778 | 5.4:+74439945 | MS.gene020712:CDS |
| AAAGACAAGCTAAGGTACAT+CGG | 0.593674 | 5.4:-74440729 | None:intergenic |
| AGATTACTTAATGAGTCTTG+GGG | 0.595406 | 5.4:+74439970 | MS.gene020712:CDS |
| AGAATCTCTTCAGACAACTC+CGG | 0.598051 | 5.4:-74439815 | None:intergenic |
| GTGCTTAAGTAATCAAGAAG+TGG | 0.598694 | 5.4:-74439857 | None:intergenic |
| GCAGAAGCATGATTAGAGGA+AGG | 0.598929 | 5.4:-74438384 | None:intergenic |
| TCTTCATCATCAGAGTCATA+CGG | 0.598960 | 5.4:-74438513 | None:intergenic |
| CATCAGAGTCATACGGAGAG+AGG | 0.602302 | 5.4:-74438506 | None:intergenic |
| ATACTGTAATGCAGAAGCTG+AGG | 0.609678 | 5.4:-74440361 | None:intergenic |
| ACCGTGTATTCTAGAAGTTG+TGG | 0.612066 | 5.4:-74440031 | None:intergenic |
| ACAAGAAAATAACAGTGCAA+AGG | 0.615006 | 5.4:+74439397 | MS.gene020712:CDS |
| TACTGTAATGCAGAAGCTGA+GGG | 0.620289 | 5.4:-74440360 | None:intergenic |
| TCCATGAAATGTCAATACGT+TGG | 0.626915 | 5.4:-74440264 | None:intergenic |
| GGTGTGAGATCTCTTAACAA+TGG | 0.634820 | 5.4:+74439332 | MS.gene020712:CDS |
| AATTCATCCAAATTTATGAG+AGG | 0.636805 | 5.4:+74439900 | MS.gene020712:CDS |
| GAATGTTGAAAGGATTGTAA+GGG | 0.639767 | 5.4:-74438353 | None:intergenic |
| GGTAAATGAACTTCACAATG+AGG | 0.640731 | 5.4:+74440686 | MS.gene020712:CDS |
| AATCTTGACTAGCCTTACAC+AGG | 0.642412 | 5.4:+74440453 | MS.gene020712:CDS |
| ACCACAACTTCTAGAATACA+CGG | 0.655167 | 5.4:+74440030 | MS.gene020712:CDS |
| GTTGGAACTAAAGATCCAAG+AGG | 0.706322 | 5.4:-74440819 | None:intergenic |
| GAAACCAAGAACTTATGACG+AGG | 0.725904 | 5.4:-74440768 | None:intergenic |
| ACAACTTCTAGAATACACGG+TGG | 0.811670 | 5.4:+74440033 | MS.gene020712:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCTACTAAAATTAAAAAT+TGG | - | chr5.4:74438706-74438725 | None:intergenic | 10.0% |
| !! | AAAATACTAAAATTTCATCA+TGG | - | chr5.4:74438658-74438677 | None:intergenic | 15.0% |
| !! | AGTAGTTATATTATGTTATT+AGG | + | chr5.4:74439490-74439509 | MS.gene020712:intron | 15.0% |
| !! | ATAATATAACTACTAATAAG+AGG | - | chr5.4:74439484-74439503 | None:intergenic | 15.0% |
| !! | ATATATTTGAATTGAAGTAA+TGG | + | chr5.4:74438786-74438805 | MS.gene020712:intron | 15.0% |
| !! | CATTGTAATTATATGTATTT+AGG | + | chr5.4:74439674-74439693 | MS.gene020712:intron | 15.0% |
| !! | TATATTTGAATTGAAGTAAT+GGG | + | chr5.4:74438787-74438806 | MS.gene020712:intron | 15.0% |
| !!! | AGTGCATTAATTTTAATTTT+TGG | + | chr5.4:74438920-74438939 | MS.gene020712:intron | 15.0% |
| !! | AACAGTAAGATATTTAATTG+AGG | + | chr5.4:74440180-74440199 | MS.gene020712:CDS | 20.0% |
| !! | AAGGAAATGATGTTATATAA+TGG | + | chr5.4:74440534-74440553 | MS.gene020712:intron | 20.0% |
| !! | TCAATTAAATATCTTACTGT+TGG | - | chr5.4:74440181-74440200 | None:intergenic | 20.0% |
| !!! | CTTGGTAGTTTTAATTTTAA+GGG | + | chr5.4:74439062-74439081 | MS.gene020712:intron | 20.0% |
| !!! | TTGGTAGTTTTAATTTTAAG+GGG | + | chr5.4:74439063-74439082 | MS.gene020712:intron | 20.0% |
| !!! | TTTAGTAGAAAATTTGAAGT+GGG | + | chr5.4:74438715-74438734 | MS.gene020712:intron | 20.0% |
| !!! | TTTTAGTAGAAAATTTGAAG+TGG | + | chr5.4:74438714-74438733 | MS.gene020712:intron | 20.0% |
| ! | AATTCATCCAAATTTATGAG+AGG | + | chr5.4:74439900-74439919 | MS.gene020712:CDS | 25.0% |
| ! | AGATATTTAATTGAGGAAGT+TGG | + | chr5.4:74440187-74440206 | MS.gene020712:CDS | 25.0% |
| ! | ATAGATTACTTAATGAGTCT+TGG | + | chr5.4:74439968-74439987 | MS.gene020712:CDS | 25.0% |
| ! | CTTTATATTGTAGGTGTATT+TGG | + | chr5.4:74439112-74439131 | MS.gene020712:intron | 25.0% |
| ! | GTTTGAATTGGAGAAATAAA+TGG | + | chr5.4:74438868-74438887 | MS.gene020712:intron | 25.0% |
| ! | TAGATTACTTAATGAGTCTT+GGG | + | chr5.4:74439969-74439988 | MS.gene020712:CDS | 25.0% |
| ! | TTCCAAGAATAAATTTCCTA+AGG | + | chr5.4:74440398-74440417 | MS.gene020712:CDS | 25.0% |
| ! | TTTACCAAGTACAAATACTT+CGG | - | chr5.4:74440672-74440691 | None:intergenic | 25.0% |
| !! | ATAAAGTGTCTATGCTATAT+AGG | - | chr5.4:74439098-74439117 | None:intergenic | 25.0% |
| !! | ATATAAGAAGAATCCTTTTG+AGG | + | chr5.4:74440005-74440024 | MS.gene020712:CDS | 25.0% |
| !! | ATGTTTGAAAAAGTTTTGCT+TGG | + | chr5.4:74439044-74439063 | MS.gene020712:intron | 25.0% |
| !!! | GCTTGGTAGTTTTAATTTTA+AGG | + | chr5.4:74439061-74439080 | MS.gene020712:intron | 25.0% |
| AAACTCTCGAAATTCTTGAA+AGG | + | chr5.4:74439619-74439638 | MS.gene020712:intron | 30.0% | |
| AACTCTCGAAATTCTTGAAA+GGG | + | chr5.4:74439620-74439639 | MS.gene020712:intron | 30.0% | |
| AATTAATGTATGTTCTGCAC+AGG | + | chr5.4:74439940-74439959 | MS.gene020712:CDS | 30.0% | |
| ACAAGAAAATAACAGTGCAA+AGG | + | chr5.4:74439397-74439416 | MS.gene020712:CDS | 30.0% | |
| ACTAATAAGAGGTCTAACAA+TGG | - | chr5.4:74439473-74439492 | None:intergenic | 30.0% | |
| AGAATGTTGAAAGGATTGTA+AGG | - | chr5.4:74438357-74438376 | None:intergenic | 30.0% | |
| AGACAACAAGAAAAGGAAAA+GGG | + | chr5.4:74439311-74439330 | MS.gene020712:CDS | 30.0% | |
| AGATTACTTAATGAGTCTTG+GGG | + | chr5.4:74439970-74439989 | MS.gene020712:CDS | 30.0% | |
| ATGCAAAAATAACCTGTGTA+AGG | - | chr5.4:74440468-74440487 | None:intergenic | 30.0% | |
| ATTGAACTTGTGTTGTATCT+AGG | + | chr5.4:74438951-74438970 | MS.gene020712:intron | 30.0% | |
| CTCAACACTGTAAGAAAAAA+GGG | - | chr5.4:74440146-74440165 | None:intergenic | 30.0% | |
| CTCCTTAGGAAATTTATTCT+TGG | - | chr5.4:74440403-74440422 | None:intergenic | 30.0% | |
| GAATGTTGAAAGGATTGTAA+GGG | - | chr5.4:74438356-74438375 | None:intergenic | 30.0% | |
| GATGTTTCTTAACAAAGAGT+TGG | + | chr5.4:74440297-74440316 | MS.gene020712:CDS | 30.0% | |
| GCATAGACACTTTATATTGT+AGG | + | chr5.4:74439103-74439122 | MS.gene020712:intron | 30.0% | |
| TAACTGTTAGTCATGTTACA+AGG | - | chr5.4:74440587-74440606 | None:intergenic | 30.0% | |
| TACAGATAGACAACAAGAAA+AGG | + | chr5.4:74439304-74439323 | MS.gene020712:intron | 30.0% | |
| TAGACAACAAGAAAAGGAAA+AGG | + | chr5.4:74439310-74439329 | MS.gene020712:CDS | 30.0% | |
| TCTCAACACTGTAAGAAAAA+AGG | - | chr5.4:74440147-74440166 | None:intergenic | 30.0% | |
| TGACAAAATGATTCATCAGT+TGG | - | chr5.4:74440840-74440859 | None:intergenic | 30.0% | |
| TGTTACAAGGATCAAACAAA+AGG | - | chr5.4:74440574-74440593 | None:intergenic | 30.0% | |
| TTGTGAAGTTGTGTTTGAAT+TGG | + | chr5.4:74438856-74438875 | MS.gene020712:intron | 30.0% | |
| TTGTGATTCCTTAATTCCAA+AGG | - | chr5.4:74439882-74439901 | None:intergenic | 30.0% | |
| TTTCAAGAATTTCGAGAGTT+TGG | - | chr5.4:74439620-74439639 | None:intergenic | 30.0% | |
| ! | AGATTCTTTTGGATGAGAAA+TGG | + | chr5.4:74439831-74439850 | MS.gene020712:CDS | 30.0% |
| ! | ATAAATTTCCTAAGGAGCAT+AGG | + | chr5.4:74440406-74440425 | MS.gene020712:CDS | 30.0% |
| ! | CTGATGAATCATTTTGTCAA+CGG | + | chr5.4:74440841-74440860 | MS.gene020712:CDS | 30.0% |
| ! | TGATGATGAAGATGAAGAAA+TGG | + | chr5.4:74438524-74438543 | MS.gene020712:CDS | 30.0% |
| ! | TTTGGGACTGAATATTGATT+GGG | + | chr5.4:74439589-74439608 | MS.gene020712:intron | 30.0% |
| !! | AACAGTGCAAAGGTATTAAA+TGG | + | chr5.4:74439407-74439426 | MS.gene020712:intron | 30.0% |
| !! | AGCAATAATTTGCCCTATTT+TGG | - | chr5.4:74440113-74440132 | None:intergenic | 30.0% |
| !!! | CGATTGGTTTTGAGAATTTT+GGG | - | chr5.4:74438465-74438484 | None:intergenic | 30.0% |
| !!! | TCGATTGGTTTTGAGAATTT+TGG | - | chr5.4:74438466-74438485 | None:intergenic | 30.0% |
| !!! | TGCACTGTTATTTTCTTGTA+AGG | - | chr5.4:74439396-74439415 | None:intergenic | 30.0% |
| !!! | TTTTGGGACTGAATATTGAT+TGG | + | chr5.4:74439588-74439607 | MS.gene020712:intron | 30.0% |
| AAAGACAAGCTAAGGTACAT+CGG | - | chr5.4:74440732-74440751 | None:intergenic | 35.0% | |
| AACTTGTGTTGTATCTAGGA+CGG | + | chr5.4:74438955-74438974 | MS.gene020712:intron | 35.0% | |
| ACACTGTAAGAAAAAAGGGA+CGG | - | chr5.4:74440142-74440161 | None:intergenic | 35.0% | |
| ACATGTGCCTCTCATAAATT+TGG | - | chr5.4:74439910-74439929 | None:intergenic | 35.0% | |
| ACTAAAGATCCAAGAGGAAA+TGG | - | chr5.4:74440816-74440835 | None:intergenic | 35.0% | |
| ACTAATTCACACACTGACAA+TGG | - | chr5.4:74439242-74439261 | None:intergenic | 35.0% | |
| ATACGTTGGACAAGAATTTG+AGG | - | chr5.4:74440253-74440272 | None:intergenic | 35.0% | |
| ATCTTCACAATGCTCTCTTT+GGG | - | chr5.4:74440328-74440347 | None:intergenic | 35.0% | |
| CTTGATTACTTAAGCACCTT+TGG | + | chr5.4:74439863-74439882 | MS.gene020712:CDS | 35.0% | |
| GAGCATAGGAATGAAAAATG+CGG | + | chr5.4:74440420-74440439 | MS.gene020712:CDS | 35.0% | |
| GGAATTGGAAGAATGTTGAA+AGG | - | chr5.4:74438366-74438385 | None:intergenic | 35.0% | |
| GGCATCAAGGAAAAAGATTT+GGG | + | chr5.4:74440208-74440227 | MS.gene020712:CDS | 35.0% | |
| GGTAAATGAACTTCACAATG+AGG | + | chr5.4:74440686-74440705 | MS.gene020712:CDS | 35.0% | |
| GTAGTTTCGTAGAAATTCGA+TGG | + | chr5.4:74439781-74439800 | MS.gene020712:intron | 35.0% | |
| GTGCTTAAGTAATCAAGAAG+TGG | - | chr5.4:74439860-74439879 | None:intergenic | 35.0% | |
| TATTGAATGCCCAAAAGTCA+GGG | + | chr5.4:74439557-74439576 | MS.gene020712:intron | 35.0% | |
| TCCATGAAATGTCAATACGT+TGG | - | chr5.4:74440267-74440286 | None:intergenic | 35.0% | |
| TCTTCATCATCAGAGTCATA+CGG | - | chr5.4:74438516-74438535 | None:intergenic | 35.0% | |
| TCTTTGGCATTAAGATCAGT+AGG | - | chr5.4:74438991-74439010 | None:intergenic | 35.0% | |
| TGACTTCAATCTCTTGTCTT+TGG | - | chr5.4:74439007-74439026 | None:intergenic | 35.0% | |
| TGGCATCAAGGAAAAAGATT+TGG | + | chr5.4:74440207-74440226 | MS.gene020712:CDS | 35.0% | |
| TTATTCTCCCTTTGCACAAA+AGG | + | chr5.4:74440502-74440521 | MS.gene020712:intron | 35.0% | |
| TTATTGAATGCCCAAAAGTC+AGG | + | chr5.4:74439556-74439575 | MS.gene020712:intron | 35.0% | |
| TTCGATGGAGAAAAGATATC+CGG | + | chr5.4:74439796-74439815 | MS.gene020712:CDS | 35.0% | |
| ! | AAAGCCGAAGTATTTGTACT+TGG | + | chr5.4:74440665-74440684 | MS.gene020712:CDS | 35.0% |
| ! | AATAAAATGCCCCTGACTTT+TGG | - | chr5.4:74439570-74439589 | None:intergenic | 35.0% |
| ! | ACTCACGTTGCTTTCTTAAT+GGG | + | chr5.4:74440070-74440089 | MS.gene020712:CDS | 35.0% |
| ! | ATAAAATGCCCCTGACTTTT+GGG | - | chr5.4:74439569-74439588 | None:intergenic | 35.0% |
| ! | ATGAATCATTTTGTCAACGG+TGG | + | chr5.4:74440844-74440863 | MS.gene020712:CDS | 35.0% |
| ! | GATGCTATTCTCTGACTTTT+GGG | - | chr5.4:74439374-74439393 | None:intergenic | 35.0% |
| ! | GTTGTCTGAAGAGATTCTTT+TGG | + | chr5.4:74439820-74439839 | MS.gene020712:CDS | 35.0% |
| ! | TCCAACGTATTGACATTTCA+TGG | + | chr5.4:74440263-74440282 | MS.gene020712:CDS | 35.0% |
| ! | TCTCTAAAGAAAGCTCCAAA+AGG | + | chr5.4:74440789-74440808 | MS.gene020712:CDS | 35.0% |
| ! | TGAATCATTTTGTCAACGGT+GGG | + | chr5.4:74440845-74440864 | MS.gene020712:CDS | 35.0% |
| ! | TTTTCATTCCTATGCTCCTT+AGG | - | chr5.4:74440417-74440436 | None:intergenic | 35.0% |
| !! | ACCACAACTTCTAGAATACA+CGG | + | chr5.4:74440030-74440049 | MS.gene020712:CDS | 35.0% |
| !! | CTGCATTACAGTATTGATGA+TGG | + | chr5.4:74440370-74440389 | MS.gene020712:CDS | 35.0% |
| !! | CTTAAGCACCTTTGGAATTA+AGG | + | chr5.4:74439871-74439890 | MS.gene020712:CDS | 35.0% |
| !!! | AAGTCAGGGGCATTTTATTT+TGG | + | chr5.4:74439571-74439590 | MS.gene020712:intron | 35.0% |
| !!! | AGTCAGGGGCATTTTATTTT+GGG | + | chr5.4:74439572-74439591 | MS.gene020712:intron | 35.0% |
| !!! | ATTTGCCCTATTTTGGTACT+AGG | - | chr5.4:74440106-74440125 | None:intergenic | 35.0% |
| !!! | GGTTTTGAGAATTTTGGGAT+TGG | - | chr5.4:74438460-74438479 | None:intergenic | 35.0% |
| AAGACAAGCTAAGGTACATC+GGG | - | chr5.4:74440731-74440750 | None:intergenic | 40.0% | |
| AATTGAGGAAGTTGGCATCA+AGG | + | chr5.4:74440195-74440214 | MS.gene020712:CDS | 40.0% | |
| ACCAATCGAAAATCACCCTA+TGG | + | chr5.4:74438477-74438496 | MS.gene020712:CDS | 40.0% | |
| AGAATCTCTTCAGACAACTC+CGG | - | chr5.4:74439818-74439837 | None:intergenic | 40.0% | |
| AGCACCACAGGTACATTAAT+TGG | - | chr5.4:74439726-74439745 | None:intergenic | 40.0% | |
| ATAAGATTCCATAGCACCAC+AGG | - | chr5.4:74439738-74439757 | None:intergenic | 40.0% | |
| ATACTGTAATGCAGAAGCTG+AGG | - | chr5.4:74440364-74440383 | None:intergenic | 40.0% | |
| ATGTATGTTCTGCACAGGAA+AGG | + | chr5.4:74439945-74439964 | MS.gene020712:CDS | 40.0% | |
| ATTGAATGCCCAAAAGTCAG+GGG | + | chr5.4:74439558-74439577 | MS.gene020712:intron | 40.0% | |
| CATCTTCACAATGCTCTCTT+TGG | - | chr5.4:74440329-74440348 | None:intergenic | 40.0% | |
| GAAACCAAGAACTTATGACG+AGG | - | chr5.4:74440771-74440790 | None:intergenic | 40.0% | |
| GCACAAAAGGAACAAGGAAA+AGG | + | chr5.4:74440515-74440534 | MS.gene020712:intron | 40.0% | |
| GCATGATTAGAGGAAGGAAT+TGG | - | chr5.4:74438381-74438400 | None:intergenic | 40.0% | |
| GCGTACCTAGTACCAAAATA+GGG | + | chr5.4:74440098-74440117 | MS.gene020712:CDS | 40.0% | |
| GCTGCAGTTATTGTGTGATT+AGG | + | chr5.4:74439512-74439531 | MS.gene020712:intron | 40.0% | |
| GGTGTGAGATCTCTTAACAA+TGG | + | chr5.4:74439332-74439351 | MS.gene020712:CDS | 40.0% | |
| GTTGGAACTAAAGATCCAAG+AGG | - | chr5.4:74440822-74440841 | None:intergenic | 40.0% | |
| TACTGTAATGCAGAAGCTGA+GGG | - | chr5.4:74440363-74440382 | None:intergenic | 40.0% | |
| TGTCAGACACTAACACGTTA+TGG | - | chr5.4:74439141-74439160 | None:intergenic | 40.0% | |
| TTATTGTGTGATTAGGCAGC+AGG | + | chr5.4:74439519-74439538 | MS.gene020712:intron | 40.0% | |
| TTGAAGTCAAAGACAGTTGC+AGG | + | chr5.4:74439018-74439037 | MS.gene020712:CDS | 40.0% | |
| TTGAATGTAACCACACGAGT+CGG | - | chr5.4:74439169-74439188 | None:intergenic | 40.0% | |
| ! | ACCGTGTATTCTAGAAGTTG+TGG | - | chr5.4:74440034-74440053 | None:intergenic | 40.0% |
| ! | AGAAGCAGAAGCATGATTAG+AGG | - | chr5.4:74438391-74438410 | None:intergenic | 40.0% |
| ! | CCTTGTTCCTTTTGTGCAAA+GGG | - | chr5.4:74440512-74440531 | None:intergenic | 40.0% |
| ! | GACTCACGTTGCTTTCTTAA+TGG | + | chr5.4:74440069-74440088 | MS.gene020712:CDS | 40.0% |
| ! | GGAGAGAGGAATTTTCCATA+GGG | - | chr5.4:74438495-74438514 | None:intergenic | 40.0% |
| ! | GGATGCTATTCTCTGACTTT+TGG | - | chr5.4:74439375-74439394 | None:intergenic | 40.0% |
| ! | TCCATAGGGTGATTTTCGAT+TGG | - | chr5.4:74438481-74438500 | None:intergenic | 40.0% |
| ! | TCCTTGTTCCTTTTGTGCAA+AGG | - | chr5.4:74440513-74440532 | None:intergenic | 40.0% |
| ! | TTAATGTACCTGTGGTGCTA+TGG | + | chr5.4:74439727-74439746 | MS.gene020712:intron | 40.0% |
| !! | AATCTTGACTAGCCTTACAC+AGG | + | chr5.4:74440453-74440472 | MS.gene020712:CDS | 40.0% |
| !! | ACAACTTCTAGAATACACGG+TGG | + | chr5.4:74440033-74440052 | MS.gene020712:CDS | 40.0% |
| !! | CAAAGAGAGCATTGTGAAGA+TGG | + | chr5.4:74440327-74440346 | MS.gene020712:CDS | 40.0% |
| !! | GATGTACCTTAGCTTGTCTT+TGG | + | chr5.4:74440731-74440750 | MS.gene020712:CDS | 40.0% |
| !! | GTTGCTTTCTTAATGGGCTT+GGG | + | chr5.4:74440076-74440095 | MS.gene020712:CDS | 40.0% |
| !!! | ACCCACAACTCGAGTTTTTT+GGG | + | chr5.4:74439647-74439666 | MS.gene020712:intron | 40.0% |
| CCAAAAGGACCATTTCCTCT+TGG | + | chr5.4:74440804-74440823 | MS.gene020712:CDS | 45.0% | |
| CCCTTTGCACAAAAGGAACA+AGG | + | chr5.4:74440509-74440528 | MS.gene020712:intron | 45.0% | |
| GAAGTTGTGGTTGCCTCAAA+AGG | - | chr5.4:74440021-74440040 | None:intergenic | 45.0% | |
| GCAGCCAATTAATGTACCTG+TGG | + | chr5.4:74439719-74439738 | MS.gene020712:intron | 45.0% | |
| GCTAAGGTACATCGGGTATT+TGG | - | chr5.4:74440724-74440743 | None:intergenic | 45.0% | |
| GGCGTACCTAGTACCAAAAT+AGG | + | chr5.4:74440097-74440116 | MS.gene020712:CDS | 45.0% | |
| GTACATCGGGTATTTGGTCA+AGG | - | chr5.4:74440718-74440737 | None:intergenic | 45.0% | |
| TATGACGAGGACGAATTCTC+TGG | - | chr5.4:74440758-74440777 | None:intergenic | 45.0% | |
| TCGTCCTCGTCATAAGTTCT+TGG | + | chr5.4:74440764-74440783 | MS.gene020712:CDS | 45.0% | |
| TCTGGTCCAAAGACAAGCTA+AGG | - | chr5.4:74440740-74440759 | None:intergenic | 45.0% | |
| ! | CCAAGAGGAAATGGTCCTTT+TGG | - | chr5.4:74440807-74440826 | None:intergenic | 45.0% |
| ! | CCCCAAAAAACTCGAGTTGT+GGG | - | chr5.4:74439651-74439670 | None:intergenic | 45.0% |
| ! | CGGAGAGAGGAATTTTCCAT+AGG | - | chr5.4:74438496-74438515 | None:intergenic | 45.0% |
| ! | GCAGAAGCATGATTAGAGGA+AGG | - | chr5.4:74438387-74438406 | None:intergenic | 45.0% |
| ! | TCCCCAAAAAACTCGAGTTG+TGG | - | chr5.4:74439652-74439671 | None:intergenic | 45.0% |
| ! | TTATTGACTACTGTGCCTGC+AGG | + | chr5.4:74440614-74440633 | MS.gene020712:intron | 45.0% |
| !! | CGGCTTTAGATTAGCTTCCA+AGG | - | chr5.4:74440652-74440671 | None:intergenic | 45.0% |
| !! | CGTTGCTTTCTTAATGGGCT+TGG | + | chr5.4:74440075-74440094 | MS.gene020712:CDS | 45.0% |
| !!! | CCCACAACTCGAGTTTTTTG+GGG | + | chr5.4:74439648-74439667 | MS.gene020712:intron | 45.0% |
| !!! | GACCCACAACTCGAGTTTTT+TGG | + | chr5.4:74439646-74439665 | MS.gene020712:intron | 45.0% |
| CATCAGAGTCATACGGAGAG+AGG | - | chr5.4:74438509-74438528 | None:intergenic | 50.0% | |
| ! | TCATTTTGTCAACGGTGGGC+TGG | + | chr5.4:74440849-74440868 | MS.gene020712:CDS | 50.0% |
| AGGACAGAGATAGCACCTGC+AGG | - | chr5.4:74440632-74440651 | None:intergenic | 55.0% | |
| ! | GCAGGTGCTATCTCTGTCCT+TGG | + | chr5.4:74440632-74440651 | MS.gene020712:intron | 55.0% |
| !! | TGACACAACACCGACTCGTG+TGG | + | chr5.4:74439156-74439175 | MS.gene020712:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 74438327 | 74440953 | 74438327 | ID=MS.gene020712 |
| chr5.4 | mRNA | 74438327 | 74440953 | 74438327 | ID=MS.gene020712.t1;Parent=MS.gene020712 |
| chr5.4 | exon | 74438327 | 74438641 | 74438327 | ID=MS.gene020712.t1.exon1;Parent=MS.gene020712.t1 |
| chr5.4 | CDS | 74438327 | 74438641 | 74438327 | ID=cds.MS.gene020712.t1;Parent=MS.gene020712.t1 |
| chr5.4 | exon | 74438973 | 74439039 | 74438973 | ID=MS.gene020712.t1.exon2;Parent=MS.gene020712.t1 |
| chr5.4 | CDS | 74438973 | 74439039 | 74438973 | ID=cds.MS.gene020712.t1;Parent=MS.gene020712.t1 |
| chr5.4 | exon | 74439309 | 74439418 | 74439309 | ID=MS.gene020712.t1.exon3;Parent=MS.gene020712.t1 |
| chr5.4 | CDS | 74439309 | 74439418 | 74439309 | ID=cds.MS.gene020712.t1;Parent=MS.gene020712.t1 |
| chr5.4 | exon | 74439785 | 74440474 | 74439785 | ID=MS.gene020712.t1.exon4;Parent=MS.gene020712.t1 |
| chr5.4 | CDS | 74439785 | 74440474 | 74439785 | ID=cds.MS.gene020712.t1;Parent=MS.gene020712.t1 |
| chr5.4 | exon | 74440636 | 74440953 | 74440636 | ID=MS.gene020712.t1.exon5;Parent=MS.gene020712.t1 |
| chr5.4 | CDS | 74440636 | 74440953 | 74440636 | ID=cds.MS.gene020712.t1;Parent=MS.gene020712.t1 |
| Gene Sequence |
| Protein sequence |
