Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020844.t1 | KEH28470.1 | 93.4 | 271 | 18 | 0 | 1 | 271 | 1 | 271 | 2.40E-138 | 501.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020844.t1 | Q680K8 | 54.8 | 270 | 121 | 1 | 1 | 270 | 59 | 327 | 3.4e-80 | 299.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020844.t1 | G7K4T7 | 93.4 | 271 | 18 | 0 | 1 | 271 | 60 | 330 | 1.7e-138 | 501.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020844.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020844.t1 | MTR_5g096700 | 93.358 | 271 | 18 | 0 | 1 | 271 | 1 | 271 | 0.0 | 520 |
| MS.gene020844.t1 | MTR_5g096700 | 93.358 | 271 | 18 | 0 | 1 | 271 | 60 | 330 | 0.0 | 519 |
| MS.gene020844.t1 | MTR_3g061090 | 65.809 | 272 | 90 | 3 | 1 | 270 | 58 | 328 | 2.94e-123 | 354 |
| MS.gene020844.t1 | MTR_3g061090 | 66.148 | 257 | 84 | 3 | 16 | 270 | 1 | 256 | 6.40e-118 | 338 |
| MS.gene020844.t1 | MTR_5g096790 | 49.624 | 266 | 118 | 6 | 6 | 266 | 68 | 322 | 1.59e-78 | 240 |
| MS.gene020844.t1 | MTR_3g088560 | 47.761 | 268 | 135 | 5 | 2 | 266 | 56 | 321 | 2.72e-78 | 240 |
| MS.gene020844.t1 | MTR_1g029850 | 43.956 | 273 | 148 | 3 | 2 | 270 | 57 | 328 | 3.55e-77 | 237 |
| MS.gene020844.t1 | MTR_3g088560 | 48.536 | 239 | 119 | 4 | 31 | 266 | 14 | 251 | 2.60e-72 | 222 |
| MS.gene020844.t1 | MTR_3g088560 | 48.523 | 237 | 118 | 4 | 33 | 266 | 31 | 266 | 2.22e-71 | 221 |
| MS.gene020844.t1 | MTR_3g088560 | 48.485 | 198 | 98 | 4 | 72 | 266 | 3 | 199 | 2.95e-57 | 182 |
| MS.gene020844.t1 | MTR_3g072160 | 34.337 | 166 | 101 | 4 | 76 | 235 | 33 | 196 | 1.39e-13 | 68.9 |
| MS.gene020844.t1 | MTR_3g072160 | 34.337 | 166 | 101 | 4 | 76 | 235 | 70 | 233 | 3.12e-13 | 68.6 |
| MS.gene020844.t1 | MTR_7g094910 | 28.462 | 130 | 91 | 2 | 106 | 235 | 526 | 653 | 8.20e-12 | 65.5 |
| MS.gene020844.t1 | MTR_3g088660 | 28.289 | 152 | 103 | 3 | 117 | 266 | 94 | 241 | 8.35e-12 | 63.9 |
| MS.gene020844.t1 | MTR_7g094900 | 28.462 | 130 | 91 | 2 | 106 | 235 | 545 | 672 | 8.43e-12 | 65.5 |
| MS.gene020844.t1 | MTR_5g005940 | 27.660 | 141 | 99 | 3 | 106 | 245 | 536 | 674 | 1.07e-11 | 65.1 |
| MS.gene020844.t1 | MTR_1g072650 | 25.749 | 167 | 112 | 2 | 80 | 235 | 185 | 350 | 2.92e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020844.t1 | AT1G55760 | 54.815 | 270 | 121 | 1 | 1 | 270 | 59 | 327 | 3.39e-104 | 306 |
| MS.gene020844.t1 | AT1G21780 | 47.970 | 271 | 138 | 3 | 1 | 270 | 56 | 324 | 2.10e-85 | 258 |
| MS.gene020844.t1 | AT1G21780 | 47.970 | 271 | 138 | 3 | 1 | 270 | 56 | 324 | 2.10e-85 | 258 |
| MS.gene020844.t1 | AT1G01640 | 30.811 | 185 | 124 | 4 | 88 | 270 | 6 | 188 | 5.17e-16 | 75.1 |
| MS.gene020844.t1 | AT1G01640 | 30.178 | 169 | 114 | 4 | 104 | 270 | 1 | 167 | 2.10e-14 | 70.1 |
| MS.gene020844.t1 | AT3G56230 | 32.847 | 137 | 89 | 3 | 102 | 235 | 107 | 243 | 3.59e-14 | 71.2 |
| MS.gene020844.t1 | AT2G30600 | 30.282 | 142 | 94 | 3 | 82 | 218 | 327 | 468 | 7.19e-13 | 68.9 |
| MS.gene020844.t1 | AT2G30600 | 30.282 | 142 | 94 | 3 | 82 | 218 | 327 | 468 | 7.19e-13 | 68.9 |
| MS.gene020844.t1 | AT2G30600 | 30.282 | 142 | 94 | 3 | 82 | 218 | 327 | 468 | 7.19e-13 | 68.9 |
| MS.gene020844.t1 | AT2G30600 | 30.282 | 142 | 94 | 3 | 82 | 218 | 327 | 468 | 7.19e-13 | 68.9 |
| MS.gene020844.t1 | AT2G30600 | 30.282 | 142 | 94 | 3 | 82 | 218 | 327 | 468 | 7.95e-13 | 68.6 |
| MS.gene020844.t1 | AT2G39760 | 28.049 | 164 | 104 | 3 | 82 | 234 | 172 | 332 | 1.86e-12 | 67.0 |
| MS.gene020844.t1 | AT2G39760 | 28.049 | 164 | 104 | 3 | 82 | 234 | 172 | 332 | 2.43e-12 | 66.6 |
| MS.gene020844.t1 | AT2G39760 | 28.049 | 164 | 104 | 3 | 82 | 234 | 172 | 332 | 2.43e-12 | 66.6 |
| MS.gene020844.t1 | AT1G05690 | 26.012 | 173 | 114 | 6 | 80 | 244 | 29 | 195 | 4.25e-12 | 65.9 |
| MS.gene020844.t1 | AT2G39760 | 28.049 | 164 | 104 | 3 | 82 | 234 | 172 | 332 | 5.29e-12 | 65.9 |
| MS.gene020844.t1 | AT4G08455 | 37.500 | 96 | 55 | 2 | 118 | 211 | 107 | 199 | 1.37e-11 | 63.5 |
| MS.gene020844.t1 | AT2G30600 | 29.452 | 146 | 93 | 4 | 82 | 222 | 327 | 467 | 1.89e-11 | 64.7 |
Find 39 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAATTGACATTGAGTTTC+TGG | 0.099574 | 5.4:+75779728 | MS.gene020844:CDS |
| GTGCAATATCTTGTGAAATT+TGG | 0.214576 | 5.4:+75780511 | MS.gene020844:CDS |
| ATTGATACAAAGATGCAATT+TGG | 0.251499 | 5.4:-75780465 | None:intergenic |
| TGACATGTCGGTGATGTTTA+TGG | 0.257723 | 5.4:-75780263 | None:intergenic |
| GTGGGGAAGCTTGCTCTATT+TGG | 0.265550 | 5.4:+75780038 | MS.gene020844:CDS |
| GATAGCTTTGTGTGGGAGAT+TGG | 0.337706 | 5.4:+75779684 | MS.gene020844:CDS |
| AGGATTGTGTGTGATTGTTT+TGG | 0.337739 | 5.4:-75779437 | None:intergenic |
| AGAATTTCTTATACATAGAT+TGG | 0.343658 | 5.4:+75780339 | MS.gene020844:CDS |
| ACTTGTCAGCCGCATGTAAT+AGG | 0.362904 | 5.4:-75780363 | None:intergenic |
| TCAAAGAAATTCTTGATGCT+TGG | 0.368629 | 5.4:+75780608 | MS.gene020844:CDS |
| AGATAAACTTCTTAGCAATA+AGG | 0.369571 | 5.4:+75779662 | MS.gene020844:CDS |
| AGCAAAATCTTGACTCAATC+AGG | 0.374788 | 5.4:+75779505 | MS.gene020844:CDS |
| CAATTGCATCTTTCATCGTT+AGG | 0.383622 | 5.4:+75779461 | MS.gene020844:CDS |
| CGTTGATTGTTATGTCTGTA+TGG | 0.409784 | 5.4:-75780135 | None:intergenic |
| AAAGAAAATTTATAAACGTC+TGG | 0.434882 | 5.4:-75780297 | None:intergenic |
| GTTAGGATTCTTAGTTATGC+AGG | 0.439632 | 5.4:+75779478 | MS.gene020844:CDS |
| TTCTCCTATCATTCGACCTA+GGG | 0.439636 | 5.4:-75780107 | None:intergenic |
| ATGAGCTCTTATGCTTCCTT+CGG | 0.452327 | 5.4:-75780164 | None:intergenic |
| GCTTGCTCTATTTGGCCTGA+TGG | 0.459699 | 5.4:+75780046 | MS.gene020844:CDS |
| CTAACGATGAAAGATGCAAT+TGG | 0.460863 | 5.4:-75779460 | None:intergenic |
| TCATTCGACCTAGGGATTCA+AGG | 0.469041 | 5.4:-75780099 | None:intergenic |
| GAATCCCTAGGTCGAATGAT+AGG | 0.475257 | 5.4:+75780103 | MS.gene020844:CDS |
| GGCAAAATATATGATATTCA+AGG | 0.488841 | 5.4:+75780532 | MS.gene020844:CDS |
| CTCGGCTGCATAAATCCATC+AGG | 0.508065 | 5.4:-75780061 | None:intergenic |
| TTTCTCCTATCATTCGACCT+AGG | 0.524682 | 5.4:-75780108 | None:intergenic |
| GAGATTGGAGTTCCATTACC+TGG | 0.545273 | 5.4:+75779699 | MS.gene020844:CDS |
| CATTCGACCTAGGGATTCAA+GGG | 0.553806 | 5.4:-75780098 | None:intergenic |
| CAATAAGGATAGCTTTGTGT+GGG | 0.566302 | 5.4:+75779677 | MS.gene020844:CDS |
| TTTGAAAATTACATGTCCAA+AGG | 0.574384 | 5.4:+75779752 | MS.gene020844:CDS |
| GCAGCGGCCCTTGAATCCCT+AGG | 0.588324 | 5.4:+75780091 | MS.gene020844:CDS |
| ACGATGAAAGATGCAATTGG+AGG | 0.589293 | 5.4:-75779457 | None:intergenic |
| GCAATAAGGATAGCTTTGTG+TGG | 0.617349 | 5.4:+75779676 | MS.gene020844:CDS |
| ACAATCAACGTCAATTCCGA+AGG | 0.618813 | 5.4:+75780148 | MS.gene020844:CDS |
| GAAAGATTGAATTGACATGT+CGG | 0.685189 | 5.4:-75780275 | None:intergenic |
| GTCAAACACAGGTGATTGTG+AGG | 0.685218 | 5.4:-75780200 | None:intergenic |
| TAGATTGGACCTATTACATG+CGG | 0.685610 | 5.4:+75780354 | MS.gene020844:CDS |
| TGATATCTATGAGTTAAGAG+AGG | 0.687061 | 5.4:+75780387 | MS.gene020844:CDS |
| GAAAACATGCTGTCAAACAC+AGG | 0.701282 | 5.4:-75780211 | None:intergenic |
| GCAGCCGAGACAAAATGCAG+CGG | 0.710020 | 5.4:+75780075 | MS.gene020844:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACAAAATATAAAGTAAA+GGG | - | chr5.4:75779907-75779926 | None:intergenic | 10.0% |
| !! | CTAAACAAAATATAAAGTAA+AGG | - | chr5.4:75779908-75779927 | None:intergenic | 15.0% |
| !!! | ACGTTTATAAATTTTCTTTA+TGG | + | chr5.4:75780301-75780320 | MS.gene020844:CDS | 15.0% |
| !!! | ATCAAGGAAAATTTTAAATT+AGG | - | chr5.4:75780007-75780026 | None:intergenic | 15.0% |
| !! | AAAGAAAATTTATAAACGTC+TGG | - | chr5.4:75780300-75780319 | None:intergenic | 20.0% |
| !! | AGAATTTCTTATACATAGAT+TGG | + | chr5.4:75780339-75780358 | MS.gene020844:CDS | 20.0% |
| !! | ATGGATAACTAATAAAAGTA+TGG | - | chr5.4:75779798-75779817 | None:intergenic | 20.0% |
| !! | ATTATATACTTACATACCTT+TGG | - | chr5.4:75779771-75779790 | None:intergenic | 20.0% |
| !!! | ATTTTCCTTGATTTGAATAA+AGG | + | chr5.4:75780015-75780034 | MS.gene020844:intron | 20.0% |
| !!! | TTTTCCTTGATTTGAATAAA+GGG | + | chr5.4:75780016-75780035 | MS.gene020844:intron | 20.0% |
| ! | AGATAAACTTCTTAGCAATA+AGG | + | chr5.4:75779662-75779681 | MS.gene020844:CDS | 25.0% |
| ! | ATTGATACAAAGATGCAATT+TGG | - | chr5.4:75780468-75780487 | None:intergenic | 25.0% |
| ! | GGCAAAATATATGATATTCA+AGG | + | chr5.4:75780532-75780551 | MS.gene020844:CDS | 25.0% |
| ! | TTTGAAAATTACATGTCCAA+AGG | + | chr5.4:75779752-75779771 | MS.gene020844:CDS | 25.0% |
| !! | ACTTTTATTAGTTATCCATG+TGG | + | chr5.4:75779799-75779818 | MS.gene020844:intron | 25.0% |
| !! | ATGTCAATTATGAATTTTCC+AGG | - | chr5.4:75779720-75779739 | None:intergenic | 25.0% |
| !! | ATTATGAATTTTCCAGGTAA+TGG | - | chr5.4:75779714-75779733 | None:intergenic | 25.0% |
| !!! | ATAATCATAGTATTGATCTG+AGG | + | chr5.4:75779958-75779977 | MS.gene020844:intron | 25.0% |
| !!! | TAATCATAGTATTGATCTGA+GGG | + | chr5.4:75779959-75779978 | MS.gene020844:intron | 25.0% |
| CATAATTGACATTGAGTTTC+TGG | + | chr5.4:75779728-75779747 | MS.gene020844:CDS | 30.0% | |
| GAAAGATTGAATTGACATGT+CGG | - | chr5.4:75780278-75780297 | None:intergenic | 30.0% | |
| GTGCAATATCTTGTGAAATT+TGG | + | chr5.4:75780511-75780530 | MS.gene020844:CDS | 30.0% | |
| TCAAAGAAATTCTTGATGCT+TGG | + | chr5.4:75780608-75780627 | MS.gene020844:CDS | 30.0% | |
| TGATATCTATGAGTTAAGAG+AGG | + | chr5.4:75780387-75780406 | MS.gene020844:CDS | 30.0% | |
| ! | CATGTTTTCACACAATGTTA+AGG | + | chr5.4:75780225-75780244 | MS.gene020844:CDS | 30.0% |
| !!! | AGTCTCTCAAGTACATTTTT+TGG | - | chr5.4:75780445-75780464 | None:intergenic | 30.0% |
| !!! | CTTTTATTTCTACATCAGTG+TGG | - | chr5.4:75779644-75779663 | None:intergenic | 30.0% |
| AGCAAAATCTTGACTCAATC+AGG | + | chr5.4:75779505-75779524 | MS.gene020844:CDS | 35.0% | |
| CAATTGCATCTTTCATCGTT+AGG | + | chr5.4:75779461-75779480 | MS.gene020844:CDS | 35.0% | |
| CTAACGATGAAAGATGCAAT+TGG | - | chr5.4:75779463-75779482 | None:intergenic | 35.0% | |
| GTTAGGATTCTTAGTTATGC+AGG | + | chr5.4:75779478-75779497 | MS.gene020844:CDS | 35.0% | |
| TAGATTGGACCTATTACATG+CGG | + | chr5.4:75780354-75780373 | MS.gene020844:CDS | 35.0% | |
| ! | AGGATTGTGTGTGATTGTTT+TGG | - | chr5.4:75779440-75779459 | None:intergenic | 35.0% |
| ! | CCTTGATTTGAATAAAGGGT+GGG | + | chr5.4:75780020-75780039 | MS.gene020844:intron | 35.0% |
| ! | CGTTGATTGTTATGTCTGTA+TGG | - | chr5.4:75780138-75780157 | None:intergenic | 35.0% |
| ! | CTTGATTTGAATAAAGGGTG+GGG | + | chr5.4:75780021-75780040 | MS.gene020844:intron | 35.0% |
| ! | TCCTTGATTTGAATAAAGGG+TGG | + | chr5.4:75780019-75780038 | MS.gene020844:intron | 35.0% |
| !! | CAATAAGGATAGCTTTGTGT+GGG | + | chr5.4:75779677-75779696 | MS.gene020844:CDS | 35.0% |
| ACAATCAACGTCAATTCCGA+AGG | + | chr5.4:75780148-75780167 | MS.gene020844:CDS | 40.0% | |
| ACGATGAAAGATGCAATTGG+AGG | - | chr5.4:75779460-75779479 | None:intergenic | 40.0% | |
| ATGAGCTCTTATGCTTCCTT+CGG | - | chr5.4:75780167-75780186 | None:intergenic | 40.0% | |
| ATTTCTGAGTCAGTACCACA+TGG | - | chr5.4:75779817-75779836 | None:intergenic | 40.0% | |
| CCCACCCTTTATTCAAATCA+AGG | - | chr5.4:75780023-75780042 | None:intergenic | 40.0% | |
| GAAAACATGCTGTCAAACAC+AGG | - | chr5.4:75780214-75780233 | None:intergenic | 40.0% | |
| TGACATGTCGGTGATGTTTA+TGG | - | chr5.4:75780266-75780285 | None:intergenic | 40.0% | |
| TTCTCCTATCATTCGACCTA+GGG | - | chr5.4:75780110-75780129 | None:intergenic | 40.0% | |
| TTTCTCCTATCATTCGACCT+AGG | - | chr5.4:75780111-75780130 | None:intergenic | 40.0% | |
| !! | GCAATAAGGATAGCTTTGTG+TGG | + | chr5.4:75779676-75779695 | MS.gene020844:CDS | 40.0% |
| ACTTGTCAGCCGCATGTAAT+AGG | - | chr5.4:75780366-75780385 | None:intergenic | 45.0% | |
| CATTCGACCTAGGGATTCAA+GGG | - | chr5.4:75780101-75780120 | None:intergenic | 45.0% | |
| GAATCCCTAGGTCGAATGAT+AGG | + | chr5.4:75780103-75780122 | MS.gene020844:CDS | 45.0% | |
| GAGATTGGAGTTCCATTACC+TGG | + | chr5.4:75779699-75779718 | MS.gene020844:CDS | 45.0% | |
| GTCAAACACAGGTGATTGTG+AGG | - | chr5.4:75780203-75780222 | None:intergenic | 45.0% | |
| TCATTCGACCTAGGGATTCA+AGG | - | chr5.4:75780102-75780121 | None:intergenic | 45.0% | |
| !! | GATAGCTTTGTGTGGGAGAT+TGG | + | chr5.4:75779684-75779703 | MS.gene020844:CDS | 45.0% |
| CTCGGCTGCATAAATCCATC+AGG | - | chr5.4:75780064-75780083 | None:intergenic | 50.0% | |
| GCTTGCTCTATTTGGCCTGA+TGG | + | chr5.4:75780046-75780065 | MS.gene020844:CDS | 50.0% | |
| ! | GTGGGGAAGCTTGCTCTATT+TGG | + | chr5.4:75780038-75780057 | MS.gene020844:CDS | 50.0% |
| GCAGCCGAGACAAAATGCAG+CGG | + | chr5.4:75780075-75780094 | MS.gene020844:CDS | 55.0% | |
| ! | AGGGCCGCTGCATTTTGTCT+CGG | - | chr5.4:75780082-75780101 | None:intergenic | 55.0% |
| GCAGCGGCCCTTGAATCCCT+AGG | + | chr5.4:75780091-75780110 | MS.gene020844:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 75779433 | 75780639 | 75779433 | ID=MS.gene020844 |
| chr5.4 | mRNA | 75779433 | 75780639 | 75779433 | ID=MS.gene020844.t1;Parent=MS.gene020844 |
| chr5.4 | exon | 75779433 | 75779526 | 75779433 | ID=MS.gene020844.t1.exon1;Parent=MS.gene020844.t1 |
| chr5.4 | CDS | 75779433 | 75779526 | 75779433 | ID=cds.MS.gene020844.t1;Parent=MS.gene020844.t1 |
| chr5.4 | exon | 75779655 | 75779773 | 75779655 | ID=MS.gene020844.t1.exon2;Parent=MS.gene020844.t1 |
| chr5.4 | CDS | 75779655 | 75779773 | 75779655 | ID=cds.MS.gene020844.t1;Parent=MS.gene020844.t1 |
| chr5.4 | exon | 75780037 | 75780639 | 75780037 | ID=MS.gene020844.t1.exon3;Parent=MS.gene020844.t1 |
| chr5.4 | CDS | 75780037 | 75780639 | 75780037 | ID=cds.MS.gene020844.t1;Parent=MS.gene020844.t1 |
| Gene Sequence |
| Protein sequence |
