Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020848.t1 | AET00865.1 | 91 | 323 | 18 | 1 | 1 | 312 | 1 | 323 | 6.20E-162 | 580.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020848.t1 | Q680K8 | 42.9 | 331 | 155 | 9 | 4 | 311 | 4 | 323 | 2.7e-57 | 223.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020848.t1 | G7K4U5 | 91.0 | 323 | 18 | 1 | 1 | 312 | 1 | 323 | 4.4e-162 | 580.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020848.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020848.t1 | MTR_5g096790 | 91.022 | 323 | 18 | 1 | 1 | 312 | 1 | 323 | 0.0 | 599 |
| MS.gene020848.t1 | MTR_5g096700 | 44.512 | 328 | 150 | 10 | 6 | 311 | 8 | 325 | 6.18e-80 | 246 |
| MS.gene020848.t1 | MTR_3g061090 | 45.808 | 334 | 148 | 9 | 1 | 311 | 1 | 324 | 5.40e-79 | 243 |
| MS.gene020848.t1 | MTR_5g096700 | 48.120 | 266 | 122 | 6 | 57 | 311 | 6 | 266 | 1.94e-73 | 227 |
| MS.gene020848.t1 | MTR_3g061090 | 49.027 | 257 | 114 | 5 | 67 | 311 | 1 | 252 | 2.13e-68 | 214 |
| MS.gene020848.t1 | MTR_1g029850 | 34.242 | 330 | 182 | 10 | 7 | 311 | 5 | 324 | 2.49e-52 | 175 |
| MS.gene020848.t1 | MTR_3g088560 | 35.758 | 330 | 175 | 11 | 7 | 311 | 4 | 321 | 2.68e-52 | 175 |
| MS.gene020848.t1 | MTR_3g088560 | 40.260 | 231 | 120 | 5 | 95 | 311 | 25 | 251 | 1.60e-44 | 152 |
| MS.gene020848.t1 | MTR_3g088560 | 40.260 | 231 | 120 | 5 | 95 | 311 | 40 | 266 | 3.66e-44 | 152 |
| MS.gene020848.t1 | MTR_3g088560 | 43.333 | 180 | 97 | 3 | 135 | 311 | 22 | 199 | 7.06e-41 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020848.t1 | AT1G55760 | 42.814 | 334 | 157 | 9 | 1 | 311 | 1 | 323 | 2.45e-74 | 231 |
| MS.gene020848.t1 | AT1G21780 | 38.110 | 328 | 168 | 10 | 7 | 311 | 5 | 320 | 2.03e-57 | 188 |
| MS.gene020848.t1 | AT1G21780 | 38.110 | 328 | 168 | 10 | 7 | 311 | 5 | 320 | 2.03e-57 | 188 |
Find 48 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCAATATCTAGTGGAATT+TGG | 0.235508 | 5.4:-75924288 | MS.gene020848:CDS |
| TTGGTAAAATACATGAAATC+TGG | 0.300649 | 5.4:-75924269 | MS.gene020848:CDS |
| GATTTGCAGTCTTGAGATCT+AGG | 0.320253 | 5.4:+75924918 | None:intergenic |
| TCAAGATATCTAAATGGAAT+TGG | 0.352470 | 5.4:-75925858 | MS.gene020848:intron |
| GCGAAACTGATGCATTTAAC+TGG | 0.358814 | 5.4:-75924994 | MS.gene020848:CDS |
| AAGCTTTCAAGATATCTAAA+TGG | 0.359355 | 5.4:-75925864 | MS.gene020848:CDS |
| GAATCATAGTTTCAAGCTAT+TGG | 0.363729 | 5.4:-75924451 | MS.gene020848:CDS |
| TATGTTTGCACAAGATTCTA+AGG | 0.372257 | 5.4:-75924574 | MS.gene020848:CDS |
| CCCACGGATTCCACTTTGAA+TGG | 0.379615 | 5.4:+75925958 | None:intergenic |
| GCATATAATTTGATAGTAAC+CGG | 0.381945 | 5.4:+75925581 | None:intergenic |
| TTTAGATATCTTGAAAGCTT+CGG | 0.402228 | 5.4:+75925867 | None:intergenic |
| ATGAATGCTTGACAAACTTC+AGG | 0.409567 | 5.4:+75924513 | None:intergenic |
| ATCTAGCTGTTCTTGCTGCC+CGG | 0.416939 | 5.4:-75924614 | MS.gene020848:CDS |
| CGGAATTTGAAAGATGCAAT+TGG | 0.422404 | 5.4:+75925536 | None:intergenic |
| CCGTGGGTTACGTCGCTCAA+TGG | 0.441645 | 5.4:-75925942 | MS.gene020848:CDS |
| TGCAATTGGTGGATTATTTG+TGG | 0.455591 | 5.4:+75925550 | None:intergenic |
| TTCCGATAGAGTCAAGAATA+CGG | 0.466943 | 5.4:+75925516 | None:intergenic |
| TTCCGTATTCTTGACTCTAT+CGG | 0.469882 | 5.4:-75925518 | MS.gene020848:CDS |
| TGGTAAAATACATGAAATCT+GGG | 0.484453 | 5.4:-75924268 | MS.gene020848:CDS |
| TTGGTTGCTGCTGAGAAATA+CGG | 0.484832 | 5.4:-75924432 | MS.gene020848:CDS |
| TACAATCAAGCTCGTATCAT+CGG | 0.487102 | 5.4:-75924655 | MS.gene020848:CDS |
| TGGAATAACCTTCGCAGAGA+CGG | 0.491897 | 5.4:-75925489 | MS.gene020848:CDS |
| GGAATAACCTTCGCAGAGAC+GGG | 0.508589 | 5.4:-75925488 | MS.gene020848:intron |
| AATTTGAAAGATGCAATTGG+TGG | 0.524532 | 5.4:+75925539 | None:intergenic |
| ACACAGGATGTTGTAACATC+AGG | 0.530431 | 5.4:+75924953 | None:intergenic |
| GTTAAATGCATCAGTTTCGC+TGG | 0.535589 | 5.4:+75924997 | None:intergenic |
| TTCTCTTAACATTCGAACAA+GGG | 0.536373 | 5.4:+75924695 | None:intergenic |
| TATATTCAAACATAGGTGAC+CGG | 0.536701 | 5.4:+75924595 | None:intergenic |
| CTTCCTAAAATTGGCAGATG+AGG | 0.543306 | 5.4:-75924232 | MS.gene020848:CDS |
| TTTCTCTTAACATTCGAACA+AGG | 0.556453 | 5.4:+75924694 | None:intergenic |
| TCTTGAAAGCTTCGGATGTG+TGG | 0.556978 | 5.4:+75925875 | None:intergenic |
| AGCACAACTAATCTTCAAGT+AGG | 0.558275 | 5.4:+75924308 | None:intergenic |
| TCCATTCAAAGTGGAATCCG+TGG | 0.560598 | 5.4:-75925959 | MS.gene020848:CDS |
| GCAAACATATATTCAAACAT+AGG | 0.563128 | 5.4:+75924588 | None:intergenic |
| CGAAACTGATGCATTTAACT+GGG | 0.567854 | 5.4:-75924993 | MS.gene020848:CDS |
| CGGATGTGTGGATAAAGCTG+TGG | 0.593373 | 5.4:+75925887 | None:intergenic |
| TAGGAATTCAACTTCAACAC+AGG | 0.603549 | 5.4:+75924937 | None:intergenic |
| CCATTGAGCGACGTAACCCA+CGG | 0.604930 | 5.4:+75925942 | None:intergenic |
| CGGCGTTAATGATTTGAGAG+AGG | 0.605267 | 5.4:-75924412 | MS.gene020848:CDS |
| GCACAACTAATCTTCAAGTA+GGG | 0.607204 | 5.4:+75924309 | None:intergenic |
| TAGTTGTGCTCAATATCTAG+TGG | 0.608180 | 5.4:-75924295 | MS.gene020848:CDS |
| ATAGAGAAACTAACTCAACT+AGG | 0.608240 | 5.4:-75924731 | MS.gene020848:CDS |
| GGATGTGTGGATAAAGCTGT+GGG | 0.609946 | 5.4:+75925888 | None:intergenic |
| CCATTCAAAGTGGAATCCGT+GGG | 0.610952 | 5.4:-75925958 | MS.gene020848:CDS |
| ATTGATAGAAATATGCAGCA+TGG | 0.618155 | 5.4:+75924334 | None:intergenic |
| ATATTCAAACATAGGTGACC+GGG | 0.654376 | 5.4:+75924596 | None:intergenic |
| GCTTGATTGTAGCGTCTGTG+TGG | 0.704769 | 5.4:+75924667 | None:intergenic |
| GATGTGTGGATAAAGCTGTG+GGG | 0.847745 | 5.4:+75925889 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTTCAATTATATCATTA+AGG | - | chr5.4:75924763-75924782 | MS.gene020848:intron | 10.0% |
| !!! | ATAATTTTAGTATTGATATT+AGG | - | chr5.4:75925354-75925373 | MS.gene020848:intron | 10.0% |
| !! | ATTATAAGTATATATGTTTG+TGG | + | chr5.4:75924907-75924926 | None:intergenic | 15.0% |
| !!! | TGTTTAAATTTTTTGAATGA+AGG | - | chr5.4:75925410-75925429 | MS.gene020848:intron | 15.0% |
| !!! | TTTCATAGACAAAAATTTTT+AGG | + | chr5.4:75925030-75925049 | None:intergenic | 15.0% |
| !! | ATGCTTATATCTATATGAAT+AGG | - | chr5.4:75924967-75924986 | MS.gene020848:CDS | 20.0% |
| !! | ATTGAATAAAACAAGACATA+TGG | - | chr5.4:75925067-75925086 | MS.gene020848:intron | 20.0% |
| !! | CTCTAATCATAAAAATCATA+TGG | - | chr5.4:75924405-75924424 | MS.gene020848:CDS | 20.0% |
| !! | TGCTTATATCTATATGAATA+GGG | - | chr5.4:75924968-75924987 | MS.gene020848:CDS | 20.0% |
| ! | AACAAGACATATGGTTAAAT+AGG | - | chr5.4:75925076-75925095 | MS.gene020848:intron | 25.0% |
| ! | AAGCTTTCAAGATATCTAAA+TGG | - | chr5.4:75924300-75924319 | MS.gene020848:CDS | 25.0% |
| ! | ACAGAAAAAATATACTTGAG+AGG | - | chr5.4:75925805-75925824 | MS.gene020848:intron | 25.0% |
| ! | ATCATAAAAATCATATGGTC+TGG | - | chr5.4:75924410-75924429 | MS.gene020848:CDS | 25.0% |
| ! | CTTTCAGAATGTAACAAATT+AGG | + | chr5.4:75924884-75924903 | None:intergenic | 25.0% |
| ! | GCAAACATATATTCAAACAT+AGG | + | chr5.4:75925579-75925598 | None:intergenic | 25.0% |
| ! | TCAAGATATCTAAATGGAAT+TGG | - | chr5.4:75924306-75924325 | MS.gene020848:CDS | 25.0% |
| ! | TGGTAAAATACATGAAATCT+GGG | - | chr5.4:75925896-75925915 | MS.gene020848:CDS | 25.0% |
| ! | TTACTTATAGAACTATGAGT+TGG | - | chr5.4:75924731-75924750 | MS.gene020848:CDS | 25.0% |
| ! | TTGGTAAAATACATGAAATC+TGG | - | chr5.4:75925895-75925914 | MS.gene020848:CDS | 25.0% |
| ! | TTTAGATATCTTGAAAGCTT+CGG | + | chr5.4:75924300-75924319 | None:intergenic | 25.0% |
| !! | GCATATAATTTGATAGTAAC+CGG | + | chr5.4:75924586-75924605 | None:intergenic | 25.0% |
| !!! | GTCTATGAAAATGTTGTTTT+TGG | - | chr5.4:75925040-75925059 | MS.gene020848:intron | 25.0% |
| AATTTGAAAGATGCAATTGG+TGG | + | chr5.4:75924628-75924647 | None:intergenic | 30.0% | |
| ATAGAGAAACTAACTCAACT+AGG | - | chr5.4:75925433-75925452 | MS.gene020848:intron | 30.0% | |
| ATTGATAGAAATATGCAGCA+TGG | + | chr5.4:75925833-75925852 | None:intergenic | 30.0% | |
| GAATCATAGTTTCAAGCTAT+TGG | - | chr5.4:75925713-75925732 | MS.gene020848:intron | 30.0% | |
| TACCAACGTTATGATGAAAT+CGG | + | chr5.4:75924439-75924458 | None:intergenic | 30.0% | |
| TATATTCAAACATAGGTGAC+CGG | + | chr5.4:75925572-75925591 | None:intergenic | 30.0% | |
| TATGTTTGCACAAGATTCTA+AGG | - | chr5.4:75925590-75925609 | MS.gene020848:intron | 30.0% | |
| TGATTAAATGCACGTACATT+TGG | + | chr5.4:75925271-75925290 | None:intergenic | 30.0% | |
| TTCTCTTAACATTCGAACAA+GGG | + | chr5.4:75925472-75925491 | None:intergenic | 30.0% | |
| TTTCTCTTAACATTCGAACA+AGG | + | chr5.4:75925473-75925492 | None:intergenic | 30.0% | |
| ! | TTTTCTTGCCTTCCTAAAAT+TGG | - | chr5.4:75925923-75925942 | MS.gene020848:CDS | 30.0% |
| !!! | TTTTTTACTTCCCAACACAA+AGG | - | chr5.4:75924527-75924546 | MS.gene020848:CDS | 30.0% |
| AATGATATGTGCCTTTGTGT+TGG | + | chr5.4:75924541-75924560 | None:intergenic | 35.0% | |
| AGCACAACTAATCTTCAAGT+AGG | + | chr5.4:75925859-75925878 | None:intergenic | 35.0% | |
| ATATTCAAACATAGGTGACC+GGG | + | chr5.4:75925571-75925590 | None:intergenic | 35.0% | |
| ATCCGATTTCATCATAACGT+TGG | - | chr5.4:75924434-75924453 | MS.gene020848:CDS | 35.0% | |
| ATGAATGCTTGACAAACTTC+AGG | + | chr5.4:75925654-75925673 | None:intergenic | 35.0% | |
| ATGATATGTGCCTTTGTGTT+GGG | + | chr5.4:75924540-75924559 | None:intergenic | 35.0% | |
| CGAAACTGATGCATTTAACT+GGG | - | chr5.4:75925171-75925190 | MS.gene020848:intron | 35.0% | |
| CGGAATTTGAAAGATGCAAT+TGG | + | chr5.4:75924631-75924650 | None:intergenic | 35.0% | |
| CTTGACTCTATCGGAAAAAA+TGG | - | chr5.4:75924655-75924674 | MS.gene020848:CDS | 35.0% | |
| GCACAACTAATCTTCAAGTA+GGG | + | chr5.4:75925858-75925877 | None:intergenic | 35.0% | |
| GCTCAATATCTAGTGGAATT+TGG | - | chr5.4:75925876-75925895 | MS.gene020848:CDS | 35.0% | |
| TACAATCAAGCTCGTATCAT+CGG | - | chr5.4:75925509-75925528 | MS.gene020848:CDS | 35.0% | |
| TAGGAATTCAACTTCAACAC+AGG | + | chr5.4:75925230-75925249 | None:intergenic | 35.0% | |
| TAGTTGTGCTCAATATCTAG+TGG | - | chr5.4:75925869-75925888 | MS.gene020848:CDS | 35.0% | |
| TATGAATAGGGACTATGAGT+TGG | - | chr5.4:75924980-75924999 | MS.gene020848:CDS | 35.0% | |
| TTCCGATAGAGTCAAGAATA+CGG | + | chr5.4:75924651-75924670 | None:intergenic | 35.0% | |
| TTCCGTATTCTTGACTCTAT+CGG | - | chr5.4:75924646-75924665 | MS.gene020848:CDS | 35.0% | |
| ! | CTTTTTCTTCTGCATCATTG+TGG | + | chr5.4:75925135-75925154 | None:intergenic | 35.0% |
| ! | GTTTTACTCTCCATTCAAAG+TGG | - | chr5.4:75924196-75924215 | MS.gene020848:CDS | 35.0% |
| ! | TAATTTGATAGTAACCGGAC+TGG | + | chr5.4:75924581-75924600 | None:intergenic | 35.0% |
| !! | TGCAATTGGTGGATTATTTG+TGG | + | chr5.4:75924617-75924636 | None:intergenic | 35.0% |
| AAGGCACATATCATTCTCGA+GGG | - | chr5.4:75924546-75924565 | MS.gene020848:CDS | 40.0% | |
| ACACAGGATGTTGTAACATC+AGG | + | chr5.4:75925214-75925233 | None:intergenic | 40.0% | |
| CTTCCTAAAATTGGCAGATG+AGG | - | chr5.4:75925932-75925951 | MS.gene020848:CDS | 40.0% | |
| GATTTGCAGTCTTGAGATCT+AGG | + | chr5.4:75925249-75925268 | None:intergenic | 40.0% | |
| GCGAAACTGATGCATTTAAC+TGG | - | chr5.4:75925170-75925189 | MS.gene020848:intron | 40.0% | |
| GTTAAATGCATCAGTTTCGC+TGG | + | chr5.4:75925170-75925189 | None:intergenic | 40.0% | |
| TCTGCATCATTGTGGAAAAG+AGG | + | chr5.4:75925127-75925146 | None:intergenic | 40.0% | |
| TTGGTTGCTGCTGAGAAATA+CGG | - | chr5.4:75925732-75925751 | MS.gene020848:intron | 40.0% | |
| ! | AAAGGCACATATCATTCTCG+AGG | - | chr5.4:75924545-75924564 | MS.gene020848:CDS | 40.0% |
| ! | CATCCTCATCTGCCAATTTT+AGG | + | chr5.4:75925938-75925957 | None:intergenic | 40.0% |
| !! | CTCATCTGCCAATTTTAGGA+AGG | + | chr5.4:75925934-75925953 | None:intergenic | 40.0% |
| CCATTCAAAGTGGAATCCGT+GGG | - | chr5.4:75924206-75924225 | MS.gene020848:CDS | 45.0% | |
| CGGCGTTAATGATTTGAGAG+AGG | - | chr5.4:75925752-75925771 | MS.gene020848:intron | 45.0% | |
| GATGTGTGGATAAAGCTGTG+GGG | + | chr5.4:75924278-75924297 | None:intergenic | 45.0% | |
| GGATGTGTGGATAAAGCTGT+GGG | + | chr5.4:75924279-75924298 | None:intergenic | 45.0% | |
| TCCATTCAAAGTGGAATCCG+TGG | - | chr5.4:75924205-75924224 | MS.gene020848:CDS | 45.0% | |
| TCTTGAAAGCTTCGGATGTG+TGG | + | chr5.4:75924292-75924311 | None:intergenic | 45.0% | |
| TGGAATAACCTTCGCAGAGA+CGG | - | chr5.4:75924675-75924694 | MS.gene020848:CDS | 45.0% | |
| ! | GATAGTAACCGGACTGGAAA+GGG | + | chr5.4:75924575-75924594 | None:intergenic | 45.0% |
| ! | TGATAGTAACCGGACTGGAA+AGG | + | chr5.4:75924576-75924595 | None:intergenic | 45.0% |
| !!! | TTTTTTACCCGTCTCTGCGA+AGG | + | chr5.4:75924686-75924705 | None:intergenic | 45.0% |
| ATCTAGCTGTTCTTGCTGCC+CGG | - | chr5.4:75925550-75925569 | MS.gene020848:CDS | 50.0% | |
| CCCACGGATTCCACTTTGAA+TGG | + | chr5.4:75924209-75924228 | None:intergenic | 50.0% | |
| CGGATGTGTGGATAAAGCTG+TGG | + | chr5.4:75924280-75924299 | None:intergenic | 50.0% | |
| GCTTGATTGTAGCGTCTGTG+TGG | + | chr5.4:75925500-75925519 | None:intergenic | 50.0% | |
| GGAATAACCTTCGCAGAGAC+GGG | - | chr5.4:75924676-75924695 | MS.gene020848:CDS | 50.0% | |
| CCATTGAGCGACGTAACCCA+CGG | + | chr5.4:75924225-75924244 | None:intergenic | 55.0% | |
| GAGGGATTCCCTTTCCAGTC+CGG | - | chr5.4:75924564-75924583 | MS.gene020848:CDS | 55.0% | |
| ! | CCGTGGGTTACGTCGCTCAA+TGG | - | chr5.4:75924222-75924241 | MS.gene020848:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 75924194 | 75925992 | 75924194 | ID=MS.gene020848 |
| chr5.4 | mRNA | 75924194 | 75925992 | 75924194 | ID=MS.gene020848.t1;Parent=MS.gene020848 |
| chr5.4 | exon | 75925859 | 75925992 | 75925859 | ID=MS.gene020848.t1.exon1;Parent=MS.gene020848.t1 |
| chr5.4 | CDS | 75925859 | 75925992 | 75925859 | ID=cds.MS.gene020848.t1;Parent=MS.gene020848.t1 |
| chr5.4 | exon | 75925489 | 75925604 | 75925489 | ID=MS.gene020848.t1.exon2;Parent=MS.gene020848.t1 |
| chr5.4 | CDS | 75925489 | 75925604 | 75925489 | ID=cds.MS.gene020848.t1;Parent=MS.gene020848.t1 |
| chr5.4 | exon | 75924913 | 75925040 | 75924913 | ID=MS.gene020848.t1.exon3;Parent=MS.gene020848.t1 |
| chr5.4 | CDS | 75924913 | 75925040 | 75924913 | ID=cds.MS.gene020848.t1;Parent=MS.gene020848.t1 |
| chr5.4 | exon | 75924194 | 75924754 | 75924194 | ID=MS.gene020848.t1.exon4;Parent=MS.gene020848.t1 |
| chr5.4 | CDS | 75924194 | 75924754 | 75924194 | ID=cds.MS.gene020848.t1;Parent=MS.gene020848.t1 |
| Gene Sequence |
| Protein sequence |
