AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020863

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020863.t1 MTR_5g097060 97.674 387 9 0 1 387 94 480 0.0 774
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020863.t1 AT5G35770 43.990 391 197 7 1 384 68 443 2.52e-100 305

Find 128 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACACTTCTATACGTTTAT+TGG 0.079855 5.4:+76204173 MS.gene020863:CDS
TTATTAGGGTGCATGAATTT+AGG 0.164199 5.4:+76203985 MS.gene020863:CDS
GTCTACATTGCTATTATTAA+TGG 0.164360 5.4:+76203463 MS.gene020863:CDS
TCATGGTTAAGAGGTTTATT+AGG 0.167477 5.4:+76203970 MS.gene020863:CDS
TGCCATCCTTGAACCGTTTC+CGG 0.223972 5.4:-76203682 None:intergenic
GCGTGAGTAGGTTTAGATTA+AGG 0.227131 5.4:+76203944 MS.gene020863:CDS
GGGTATGTTATAACCTATTC+TGG 0.255175 5.4:+76204006 MS.gene020863:CDS
CCTGCACGACGGGCAATTAA+TGG 0.265227 5.4:+76203511 MS.gene020863:CDS
CCTTCTCCATCATCTCTAAC+TGG 0.275457 5.4:-76204093 None:intergenic
TCCGTTTCGGGAATTGTAAT+TGG 0.284357 5.4:+76203403 MS.gene020863:CDS
TATTAGGGTGCATGAATTTA+GGG 0.289123 5.4:+76203986 MS.gene020863:CDS
GTGCACATGTTAGCACATTT+TGG 0.290721 5.4:+76203344 MS.gene020863:CDS
TATATGTTGGCCACGCGGGC+GGG 0.296183 5.4:+76203596 MS.gene020863:CDS
CGTGAGTAGGTTTAGATTAA+GGG 0.323549 5.4:+76203945 MS.gene020863:CDS
CATGGTTAAGAGGTTTATTA+GGG 0.328702 5.4:+76203971 MS.gene020863:CDS
CTCGCCTTCGCGAGATTAGA+TGG 0.334905 5.4:+76203436 MS.gene020863:CDS
GCAGGAATGGGCCCGGGTAT+TGG 0.339605 5.4:-76203493 None:intergenic
TCTACATTGCTATTATTAAT+GGG 0.340728 5.4:+76203464 MS.gene020863:CDS
TCCAACCATGGTCTTCTATT+CGG 0.353047 5.4:+76203367 MS.gene020863:CDS
ACATCACGTAATAGAATCTC+TGG 0.371896 5.4:-76203811 None:intergenic
ATAACCTATTCTGGTGGGTC+TGG 0.373210 5.4:+76204015 MS.gene020863:CDS
GGCAGCGGCAGATGAGGTTT+TGG 0.378783 5.4:-76203249 None:intergenic
ACGGAACAGCGCGTGTTTGT+TGG 0.386206 5.4:+76203643 MS.gene020863:CDS
AGGATTCATTGTAGGATCAT+TGG 0.389444 5.4:+76203846 MS.gene020863:CDS
AGCTACTACAAATTCTCTTT+CGG 0.392581 5.4:-76203749 None:intergenic
CGTGATGTAGTGTCCATAAC+AGG 0.395954 5.4:+76203826 MS.gene020863:CDS
GGTTTAGATTAAGGGCTTCA+TGG 0.398867 5.4:+76203953 MS.gene020863:CDS
TTCATTAACCGATCCGGAAA+CGG 0.410471 5.4:+76203669 MS.gene020863:CDS
CACCGGGAGACATGCTTTAG+TGG 0.414725 5.4:+76203198 MS.gene020863:CDS
CCCGAAACGGATTGTGAAAA+CGG 0.417479 5.4:-76203391 None:intergenic
AGCTTGCACAAGTACAAGAT+TGG 0.422430 5.4:+76203768 MS.gene020863:CDS
CAAACCAGACCCACCAGAAT+AGG 0.423103 5.4:-76204019 None:intergenic
ACCGACCGGTTCAACTAATT+CGG 0.424968 5.4:-76203713 None:intergenic
AGAGGCTCACGAGACGCATC+TGG 0.428365 5.4:+76203101 MS.gene020863:CDS
TCCAATTACAATTCCCGAAA+CGG 0.436742 5.4:-76203404 None:intergenic
CGGGCCCATTCCTGCACGAC+GGG 0.438206 5.4:+76203501 MS.gene020863:CDS
GGCTCTAGTCGTTGGTGGGT+TGG 0.439072 5.4:+76203571 MS.gene020863:CDS
TCCTACAATGAATCCTGTTA+TGG 0.441153 5.4:-76203839 None:intergenic
TCTCCCGGTGGTAAAGTTCC+TGG 0.441224 5.4:-76203185 None:intergenic
TCCTTGAACCGTTTCCGGAT+CGG 0.441558 5.4:-76203677 None:intergenic
CCATTAATTGCCCGTCGTGC+AGG 0.443293 5.4:-76203511 None:intergenic
TTAAGGGCTTCATGGTTAAG+AGG 0.447013 5.4:+76203961 MS.gene020863:CDS
ATATGTTGGCCACGCGGGCG+GGG 0.450684 5.4:+76203597 MS.gene020863:CDS
AATTGCCCGTCGTGCAGGAA+TGG 0.458917 5.4:-76203506 None:intergenic
ATTGCCCGTCGTGCAGGAAT+GGG 0.459648 5.4:-76203505 None:intergenic
ACCGATCCGGAAACGGTTCA+AGG 0.460400 5.4:+76203676 MS.gene020863:CDS
CCGTCGTGCAGGAATGGGCC+CGG 0.461152 5.4:-76203500 None:intergenic
ATGGGCCCGGGTATTGGTCC+AGG 0.465999 5.4:-76203487 None:intergenic
GTCTGGTTTGTTAAGAGTGT+GGG 0.468579 5.4:+76204032 MS.gene020863:CDS
GTTAACCGAATTAGTTGAAC+CGG 0.476077 5.4:+76203708 MS.gene020863:CDS
CAAATTCTCTTTCGGTGACT+CGG 0.479960 5.4:-76203741 None:intergenic
TTGCCGGCTCTAGTCGTTGG+TGG 0.480637 5.4:+76203566 MS.gene020863:CDS
TGCCGGCTCTAGTCGTTGGT+GGG 0.484033 5.4:+76203567 MS.gene020863:CDS
ATTGCTATTATTAATGGGCC+TGG 0.486088 5.4:+76203469 MS.gene020863:CDS
CGACGAAGCCGCAGGCGAGG+TGG 0.487527 5.4:-76203288 None:intergenic
GGAGTATTAGTAGAATTTGC+CGG 0.489467 5.4:+76203550 MS.gene020863:CDS
TTATATGTTGGCCACGCGGG+CGG 0.490183 5.4:+76203595 MS.gene020863:CDS
ACGCTCCCAGTTAGAGATGA+TGG 0.491914 5.4:+76204087 MS.gene020863:CDS
ATCTAATCTCGCGAAGGCGA+GGG 0.494316 5.4:-76203434 None:intergenic
TCAAATATGGGACGCGCACA+CGG 0.496233 5.4:+76203624 MS.gene020863:CDS
CCGAAACGGATTGTGAAAAC+GGG 0.497568 5.4:-76203390 None:intergenic
TGGCGGTTCATTAACCGATC+CGG 0.497586 5.4:+76203663 MS.gene020863:CDS
CATCTAATCTCGCGAAGGCG+AGG 0.498179 5.4:-76203435 None:intergenic
TGATCTCCTGGATCATATTG+TGG 0.499584 5.4:-76203223 None:intergenic
GAACAGCGCGTGTTTGTTGG+CGG 0.505393 5.4:+76203646 MS.gene020863:CDS
TGTCGGAGAGAGTGAGGCAG+CGG 0.506671 5.4:-76203264 None:intergenic
AATGGTGATGTTGTCAATAA+CGG 0.506851 5.4:+76203529 MS.gene020863:CDS
TCCATAACAGGATTCATTGT+AGG 0.509536 5.4:+76203838 MS.gene020863:CDS
CTTCTCCATCATCTCTAACT+GGG 0.510502 5.4:-76204092 None:intergenic
AACCCACCAACGACTAGAGC+CGG 0.516179 5.4:-76203569 None:intergenic
GGTCTGGTTTGTTAAGAGTG+TGG 0.519402 5.4:+76204031 MS.gene020863:CDS
CTCGCCTGCGGCTTCGTCGA+CGG 0.523192 5.4:+76203292 MS.gene020863:CDS
GAGTGAGGCAGCGGCAGATG+AGG 0.527259 5.4:-76203255 None:intergenic
GTTCGGATCTACTGTGGCAG+AGG 0.528047 5.4:+76203083 MS.gene020863:CDS
TGGTGGGTTGGTTTATATGT+TGG 0.528184 5.4:+76203583 MS.gene020863:CDS
GACGAAGCCGCAGGCGAGGT+GGG 0.529764 5.4:-76203287 None:intergenic
GGCGCAGAACATATAGATTG+AGG 0.532902 5.4:+76203122 MS.gene020863:CDS
GTGGTCCCACAATATGATCC+AGG 0.534362 5.4:+76203217 MS.gene020863:CDS
ATGTTATAACCTATTCTGGT+GGG 0.534574 5.4:+76204010 MS.gene020863:CDS
GCCGCAGGCGAGGTGGGTGT+CGG 0.536831 5.4:-76203281 None:intergenic
GTGATACACAATCGCGCCGC+AGG 0.537724 5.4:-76204066 None:intergenic
ATGGAGAGAAGTGTCACGTT+CGG 0.540623 5.4:+76203066 MS.gene020863:CDS
GCGCAGAACATATAGATTGA+GGG 0.543876 5.4:+76203123 MS.gene020863:CDS
GATCTCCTGGATCATATTGT+GGG 0.544786 5.4:-76203222 None:intergenic
CGTCGTGCAGGAATGGGCCC+GGG 0.546050 5.4:-76203499 None:intergenic
AATTTGCCGGCTCTAGTCGT+TGG 0.546832 5.4:+76203563 MS.gene020863:CDS
GGTTTATATGTTGGCCACGC+GGG 0.547159 5.4:+76203592 MS.gene020863:CDS
GTGGTGGAAAGAAGTGGTGT+TGG 0.551066 5.4:+76203892 MS.gene020863:CDS
AATGGGCCTGGACCAATACC+CGG 0.556865 5.4:+76203481 MS.gene020863:CDS
GCCGAATAGAAGACCATGGT+TGG 0.558339 5.4:-76203368 None:intergenic
GGCAGTTAGAGTACATCTAC+TGG 0.561144 5.4:+76203152 MS.gene020863:CDS
TCTACTGGCATCGAACGGCC+AGG 0.565427 5.4:+76203167 MS.gene020863:CDS
TTTGTTGTGGTGGAAAGAAG+TGG 0.565574 5.4:+76203886 MS.gene020863:CDS
GAAGTGGTGTTGGAACTGTT+CGG 0.568741 5.4:+76203902 MS.gene020863:CDS
GGTGGGTGTCGGAGAGAGTG+AGG 0.568818 5.4:-76203270 None:intergenic
CGTCGACGAAGCCGCAGGCG+AGG 0.570217 5.4:-76203291 None:intergenic
ATGGGCCTGGACCAATACCC+GGG 0.577553 5.4:+76203482 MS.gene020863:CDS
GTGACTCGGACTCGACCGAC+CGG 0.586956 5.4:-76203727 None:intergenic
ATCCGGAAACGGTTCAAGGA+TGG 0.588593 5.4:+76203680 MS.gene020863:CDS
GACCACTAAAGCATGTCTCC+CGG 0.592351 5.4:-76203200 None:intergenic
TATGTTATAACCTATTCTGG+TGG 0.596396 5.4:+76204009 MS.gene020863:CDS
GCATGCAGGTTTGTTCAACA+TGG 0.597878 5.4:+76203047 None:intergenic
ACTGCCGTCGACGAAGCCGC+AGG 0.600288 5.4:-76203296 None:intergenic
ACGGCCAGGAACTTTACCAC+CGG 0.601863 5.4:+76203181 MS.gene020863:CDS
ATAATTTCGAGCGCGTGAGT+AGG 0.604680 5.4:+76203932 MS.gene020863:CDS
CGGCCAGGAACTTTACCACC+GGG 0.607000 5.4:+76203182 MS.gene020863:CDS
CCAGTTAGAGATGATGGAGA+AGG 0.611674 5.4:+76204093 MS.gene020863:CDS
GATGGAGAAGGACAAGTACA+TGG 0.613846 5.4:+76204105 MS.gene020863:CDS
TCCGACACCCACCTCGCCTG+CGG 0.615146 5.4:+76203280 MS.gene020863:CDS
ATCTCCATCTAATCTCGCGA+AGG 0.620003 5.4:-76203440 None:intergenic
ATCATTGGATGTTAGCCATG+AGG 0.625954 5.4:+76203861 MS.gene020863:CDS
TGCTAACATGTGCACCGGTG+TGG 0.634853 5.4:-76203336 None:intergenic
TGGTTTATATGTTGGCCACG+CGG 0.637945 5.4:+76203591 MS.gene020863:CDS
ACAAGTACATGGCAATAGCA+TGG 0.638195 5.4:+76204116 MS.gene020863:CDS
CATCACGTAATAGAATCTCT+GGG 0.640810 5.4:-76203810 None:intergenic
CATATAGATTGAGGGACACG+TGG 0.643287 5.4:+76203131 MS.gene020863:CDS
CGGTGTGGAGATCGAAGAGT+CGG 0.650728 5.4:-76203321 None:intergenic
CCGGGCCCATTCCTGCACGA+CGG 0.655323 5.4:+76203500 MS.gene020863:CDS
AAATGTGCTAACATGTGCAC+CGG 0.656474 5.4:-76203341 None:intergenic
GTACATCTACTGGCATCGAA+CGG 0.656579 5.4:+76203162 MS.gene020863:CDS
TTTGAAAAGCCCCGCCCGCG+TGG 0.661088 5.4:-76203606 None:intergenic
CGACTCTTCGATCTCCACAC+CGG 0.677791 5.4:+76203322 MS.gene020863:CDS
ACCGAATTAGTTGAACCGGT+CGG 0.683053 5.4:+76203712 MS.gene020863:CDS
TGTCACGTTCGGATCTACTG+TGG 0.685634 5.4:+76203077 MS.gene020863:CDS
GTGGGACATACATGAACCTG+CGG 0.688958 5.4:+76204050 MS.gene020863:CDS
CACTAAAGCATGTCTCCCGG+TGG 0.706707 5.4:-76203197 None:intergenic
GGTTGCTAATCAAACTCACG+TGG 0.706916 5.4:+76204137 MS.gene020863:CDS
ACGGGCCGAATAGAAGACCA+TGG 0.711737 5.4:-76203372 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTACATTGCTATTATTAAT+GGG + chr5.4:76203464-76203483 MS.gene020863:CDS 20.0%
!! GTCTACATTGCTATTATTAA+TGG + chr5.4:76203463-76203482 MS.gene020863:CDS 25.0%
AGCTACTACAAATTCTCTTT+CGG - chr5.4:76203752-76203771 None:intergenic 30.0%
ATGTTATAACCTATTCTGGT+GGG + chr5.4:76204010-76204029 MS.gene020863:CDS 30.0%
CATGGTTAAGAGGTTTATTA+GGG + chr5.4:76203971-76203990 MS.gene020863:CDS 30.0%
TATGTTATAACCTATTCTGG+TGG + chr5.4:76204009-76204028 MS.gene020863:CDS 30.0%
TATTAGGGTGCATGAATTTA+GGG + chr5.4:76203986-76204005 MS.gene020863:CDS 30.0%
TCATGGTTAAGAGGTTTATT+AGG + chr5.4:76203970-76203989 MS.gene020863:CDS 30.0%
TGACACTTCTATACGTTTAT+TGG + chr5.4:76204173-76204192 MS.gene020863:CDS 30.0%
TTATTAGGGTGCATGAATTT+AGG + chr5.4:76203985-76204004 MS.gene020863:CDS 30.0%
! AATGGTGATGTTGTCAATAA+CGG + chr5.4:76203529-76203548 MS.gene020863:CDS 30.0%
ACATCACGTAATAGAATCTC+TGG - chr5.4:76203814-76203833 None:intergenic 35.0%
AGGATTCATTGTAGGATCAT+TGG + chr5.4:76203846-76203865 MS.gene020863:CDS 35.0%
CATCACGTAATAGAATCTCT+GGG - chr5.4:76203813-76203832 None:intergenic 35.0%
CGTGAGTAGGTTTAGATTAA+GGG + chr5.4:76203945-76203964 MS.gene020863:CDS 35.0%
GGGTATGTTATAACCTATTC+TGG + chr5.4:76204006-76204025 MS.gene020863:CDS 35.0%
GTTAACCGAATTAGTTGAAC+CGG + chr5.4:76203708-76203727 MS.gene020863:CDS 35.0%
TCCAATTACAATTCCCGAAA+CGG - chr5.4:76203407-76203426 None:intergenic 35.0%
TCCATAACAGGATTCATTGT+AGG + chr5.4:76203838-76203857 MS.gene020863:CDS 35.0%
TCCTACAATGAATCCTGTTA+TGG - chr5.4:76203842-76203861 None:intergenic 35.0%
! ATTGCTATTATTAATGGGCC+TGG + chr5.4:76203469-76203488 MS.gene020863:CDS 35.0%
! GGAGTATTAGTAGAATTTGC+CGG + chr5.4:76203550-76203569 MS.gene020863:CDS 35.0%
AAATGTGCTAACATGTGCAC+CGG - chr5.4:76203344-76203363 None:intergenic 40.0%
ACAAGTACATGGCAATAGCA+TGG + chr5.4:76204116-76204135 MS.gene020863:CDS 40.0%
AGCTTGCACAAGTACAAGAT+TGG + chr5.4:76203768-76203787 MS.gene020863:CDS 40.0%
ATCATTGGATGTTAGCCATG+AGG + chr5.4:76203861-76203880 MS.gene020863:CDS 40.0%
CAAATTCTCTTTCGGTGACT+CGG - chr5.4:76203744-76203763 None:intergenic 40.0%
CTTCTCCATCATCTCTAACT+GGG - chr5.4:76204095-76204114 None:intergenic 40.0%
GATCTCCTGGATCATATTGT+GGG - chr5.4:76203225-76203244 None:intergenic 40.0%
GCGCAGAACATATAGATTGA+GGG + chr5.4:76203123-76203142 MS.gene020863:CDS 40.0%
GCGTGAGTAGGTTTAGATTA+AGG + chr5.4:76203944-76203963 MS.gene020863:CDS 40.0%
GGTTTAGATTAAGGGCTTCA+TGG + chr5.4:76203953-76203972 MS.gene020863:CDS 40.0%
TCCAACCATGGTCTTCTATT+CGG + chr5.4:76203367-76203386 MS.gene020863:CDS 40.0%
TCCGTTTCGGGAATTGTAAT+TGG + chr5.4:76203403-76203422 MS.gene020863:CDS 40.0%
TGATCTCCTGGATCATATTG+TGG - chr5.4:76203226-76203245 None:intergenic 40.0%
TTAAGGGCTTCATGGTTAAG+AGG + chr5.4:76203961-76203980 MS.gene020863:CDS 40.0%
TTCATTAACCGATCCGGAAA+CGG + chr5.4:76203669-76203688 MS.gene020863:CDS 40.0%
! GTGCACATGTTAGCACATTT+TGG + chr5.4:76203344-76203363 MS.gene020863:CDS 40.0%
! TAGCCATGAGGTTTTTGTTG+TGG + chr5.4:76203873-76203892 MS.gene020863:CDS 40.0%
! TTTGTTGTGGTGGAAAGAAG+TGG + chr5.4:76203886-76203905 MS.gene020863:CDS 40.0%
!! GTCTGGTTTGTTAAGAGTGT+GGG + chr5.4:76204032-76204051 MS.gene020863:CDS 40.0%
!! TGGTGGGTTGGTTTATATGT+TGG + chr5.4:76203583-76203602 MS.gene020863:CDS 40.0%
ACCGAATTAGTTGAACCGGT+CGG + chr5.4:76203712-76203731 MS.gene020863:CDS 45.0%
ATAATTTCGAGCGCGTGAGT+AGG + chr5.4:76203932-76203951 MS.gene020863:CDS 45.0%
ATCTCCATCTAATCTCGCGA+AGG - chr5.4:76203443-76203462 None:intergenic 45.0%
CATATAGATTGAGGGACACG+TGG + chr5.4:76203131-76203150 MS.gene020863:CDS 45.0%
CCACCACAACAAAAACCTCA+TGG - chr5.4:76203879-76203898 None:intergenic 45.0%
CCTTCTCCATCATCTCTAAC+TGG - chr5.4:76204096-76204115 None:intergenic 45.0%
CGTGATGTAGTGTCCATAAC+AGG + chr5.4:76203826-76203845 MS.gene020863:CDS 45.0%
GATGGAGAAGGACAAGTACA+TGG + chr5.4:76204105-76204124 MS.gene020863:CDS 45.0%
GGCAGTTAGAGTACATCTAC+TGG + chr5.4:76203152-76203171 MS.gene020863:CDS 45.0%
GGCGCAGAACATATAGATTG+AGG + chr5.4:76203122-76203141 MS.gene020863:CDS 45.0%
GGTTGCTAATCAAACTCACG+TGG + chr5.4:76204137-76204156 MS.gene020863:CDS 45.0%
TGGTTTATATGTTGGCCACG+CGG + chr5.4:76203591-76203610 MS.gene020863:CDS 45.0%
! ACCGACCGGTTCAACTAATT+CGG - chr5.4:76203716-76203735 None:intergenic 45.0%
! ATAACCTATTCTGGTGGGTC+TGG + chr5.4:76204015-76204034 MS.gene020863:CDS 45.0%
! CCCGTTTTCACAATCCGTTT+CGG + chr5.4:76203390-76203409 MS.gene020863:CDS 45.0%
! CCGTTTTCACAATCCGTTTC+GGG + chr5.4:76203391-76203410 MS.gene020863:CDS 45.0%
! GAAGTGGTGTTGGAACTGTT+CGG + chr5.4:76203902-76203921 MS.gene020863:CDS 45.0%
! GTACATCTACTGGCATCGAA+CGG + chr5.4:76203162-76203181 MS.gene020863:CDS 45.0%
!! AGCACATTTTGGTCCAACCA+TGG + chr5.4:76203355-76203374 MS.gene020863:CDS 45.0%
!! ATGGAGAGAAGTGTCACGTT+CGG + chr5.4:76203066-76203085 MS.gene020863:CDS 45.0%
!! CCAGTTAGAGATGATGGAGA+AGG + chr5.4:76204093-76204112 MS.gene020863:CDS 45.0%
!! CCATGAGGTTTTTGTTGTGG+TGG + chr5.4:76203876-76203895 MS.gene020863:CDS 45.0%
!! CCCGAAACGGATTGTGAAAA+CGG - chr5.4:76203394-76203413 None:intergenic 45.0%
!! CCGAAACGGATTGTGAAAAC+GGG - chr5.4:76203393-76203412 None:intergenic 45.0%
!! GGTCTGGTTTGTTAAGAGTG+TGG + chr5.4:76204031-76204050 MS.gene020863:CDS 45.0%
ACGCTCCCAGTTAGAGATGA+TGG + chr5.4:76204087-76204106 MS.gene020863:CDS 50.0%
ATCCGGAAACGGTTCAAGGA+TGG + chr5.4:76203680-76203699 MS.gene020863:CDS 50.0%
ATCTAATCTCGCGAAGGCGA+GGG - chr5.4:76203437-76203456 None:intergenic 50.0%
CAAACCAGACCCACCAGAAT+AGG - chr5.4:76204022-76204041 None:intergenic 50.0%
GACCACTAAAGCATGTCTCC+CGG - chr5.4:76203203-76203222 None:intergenic 50.0%
GCCGAATAGAAGACCATGGT+TGG - chr5.4:76203371-76203390 None:intergenic 50.0%
GGTTTATATGTTGGCCACGC+GGG + chr5.4:76203592-76203611 MS.gene020863:CDS 50.0%
GTGGGACATACATGAACCTG+CGG + chr5.4:76204050-76204069 MS.gene020863:CDS 50.0%
GTGGTCCCACAATATGATCC+AGG + chr5.4:76203217-76203236 MS.gene020863:CDS 50.0%
TCAAATATGGGACGCGCACA+CGG + chr5.4:76203624-76203643 MS.gene020863:CDS 50.0%
TCCTTGAACCGTTTCCGGAT+CGG - chr5.4:76203680-76203699 None:intergenic 50.0%
TGCCATCCTTGAACCGTTTC+CGG - chr5.4:76203685-76203704 None:intergenic 50.0%
TGGCGGTTCATTAACCGATC+CGG + chr5.4:76203663-76203682 MS.gene020863:CDS 50.0%
TGTCACGTTCGGATCTACTG+TGG + chr5.4:76203077-76203096 MS.gene020863:CDS 50.0%
! GGGCGGGGCTTTTCAAATAT+GGG + chr5.4:76203612-76203631 MS.gene020863:CDS 50.0%
! GTGGTGGAAAGAAGTGGTGT+TGG + chr5.4:76203892-76203911 MS.gene020863:CDS 50.0%
!! AATTTGCCGGCTCTAGTCGT+TGG + chr5.4:76203563-76203582 MS.gene020863:CDS 50.0%
!!! AGGTTTTGGTGGTGATCTCC+TGG - chr5.4:76203238-76203257 None:intergenic 50.0%
AACCCACCAACGACTAGAGC+CGG - chr5.4:76203572-76203591 None:intergenic 55.0%
AATGGGCCTGGACCAATACC+CGG + chr5.4:76203481-76203500 MS.gene020863:CDS 55.0%
AATTGCCCGTCGTGCAGGAA+TGG - chr5.4:76203509-76203528 None:intergenic 55.0%
ACCGATCCGGAAACGGTTCA+AGG + chr5.4:76203676-76203695 MS.gene020863:CDS 55.0%
ACGGAACAGCGCGTGTTTGT+TGG + chr5.4:76203643-76203662 MS.gene020863:CDS 55.0%
ACGGCCAGGAACTTTACCAC+CGG + chr5.4:76203181-76203200 MS.gene020863:CDS 55.0%
ACGGGCCGAATAGAAGACCA+TGG - chr5.4:76203375-76203394 None:intergenic 55.0%
ATTGCCCGTCGTGCAGGAAT+GGG - chr5.4:76203508-76203527 None:intergenic 55.0%
CACCGGGAGACATGCTTTAG+TGG + chr5.4:76203198-76203217 MS.gene020863:CDS 55.0%
CACTAAAGCATGTCTCCCGG+TGG - chr5.4:76203200-76203219 None:intergenic 55.0%
CATCTAATCTCGCGAAGGCG+AGG - chr5.4:76203438-76203457 None:intergenic 55.0%
CCATTAATTGCCCGTCGTGC+AGG - chr5.4:76203514-76203533 None:intergenic 55.0%
CCTGCACGACGGGCAATTAA+TGG + chr5.4:76203511-76203530 MS.gene020863:CDS 55.0%
CGACTCTTCGATCTCCACAC+CGG + chr5.4:76203322-76203341 MS.gene020863:CDS 55.0%
CGGTGTGGAGATCGAAGAGT+CGG - chr5.4:76203324-76203343 None:intergenic 55.0%
CTCGCCTTCGCGAGATTAGA+TGG + chr5.4:76203436-76203455 MS.gene020863:CDS 55.0%
GTTCGGATCTACTGTGGCAG+AGG + chr5.4:76203083-76203102 MS.gene020863:CDS 55.0%
TCTCCCGGTGGTAAAGTTCC+TGG - chr5.4:76203188-76203207 None:intergenic 55.0%
TTATATGTTGGCCACGCGGG+CGG + chr5.4:76203595-76203614 MS.gene020863:CDS 55.0%
! CGGGCGGGGCTTTTCAAATA+TGG + chr5.4:76203611-76203630 MS.gene020863:CDS 55.0%
! GAACAGCGCGTGTTTGTTGG+CGG + chr5.4:76203646-76203665 MS.gene020863:CDS 55.0%
! TGCTAACATGTGCACCGGTG+TGG - chr5.4:76203339-76203358 None:intergenic 55.0%
!! AGCGGCAGATGAGGTTTTGG+TGG - chr5.4:76203249-76203268 None:intergenic 55.0%
AGAGGCTCACGAGACGCATC+TGG + chr5.4:76203101-76203120 MS.gene020863:CDS 60.0%
ATGGGCCTGGACCAATACCC+GGG + chr5.4:76203482-76203501 MS.gene020863:CDS 60.0%
CGGCCAGGAACTTTACCACC+GGG + chr5.4:76203182-76203201 MS.gene020863:CDS 60.0%
GTGATACACAATCGCGCCGC+AGG - chr5.4:76204069-76204088 None:intergenic 60.0%
TATATGTTGGCCACGCGGGC+GGG + chr5.4:76203596-76203615 MS.gene020863:CDS 60.0%
TGTCGGAGAGAGTGAGGCAG+CGG - chr5.4:76203267-76203286 None:intergenic 60.0%
! GGCAGCGGCAGATGAGGTTT+TGG - chr5.4:76203252-76203271 None:intergenic 60.0%
! GGCTCTAGTCGTTGGTGGGT+TGG + chr5.4:76203571-76203590 MS.gene020863:CDS 60.0%
! TCTACTGGCATCGAACGGCC+AGG + chr5.4:76203167-76203186 MS.gene020863:CDS 60.0%
!! TGCCGGCTCTAGTCGTTGGT+GGG + chr5.4:76203567-76203586 MS.gene020863:CDS 60.0%
!! TTGCCGGCTCTAGTCGTTGG+TGG + chr5.4:76203566-76203585 MS.gene020863:CDS 60.0%
ATATGTTGGCCACGCGGGCG+GGG + chr5.4:76203597-76203616 MS.gene020863:CDS 65.0%
GAGTGAGGCAGCGGCAGATG+AGG - chr5.4:76203258-76203277 None:intergenic 65.0%
GCAGGAATGGGCCCGGGTAT+TGG - chr5.4:76203496-76203515 None:intergenic 65.0%
TTTGAAAAGCCCCGCCCGCG+TGG - chr5.4:76203609-76203628 None:intergenic 65.0%
! GTGACTCGGACTCGACCGAC+CGG - chr5.4:76203730-76203749 None:intergenic 65.0%
!! ATGGGCCCGGGTATTGGTCC+AGG - chr5.4:76203490-76203509 None:intergenic 65.0%
!! GGTGGGTGTCGGAGAGAGTG+AGG - chr5.4:76203273-76203292 None:intergenic 65.0%
ACTGCCGTCGACGAAGCCGC+AGG - chr5.4:76203299-76203318 None:intergenic 70.0%
CCGGGCCCATTCCTGCACGA+CGG + chr5.4:76203500-76203519 MS.gene020863:CDS 70.0%
CCGTCGTGCAGGAATGGGCC+CGG - chr5.4:76203503-76203522 None:intergenic 70.0%
CGGGCCCATTCCTGCACGAC+GGG + chr5.4:76203501-76203520 MS.gene020863:CDS 70.0%
CGTCGTGCAGGAATGGGCCC+GGG - chr5.4:76203502-76203521 None:intergenic 70.0%
CTCGCCTGCGGCTTCGTCGA+CGG + chr5.4:76203292-76203311 MS.gene020863:CDS 70.0%
GACGAAGCCGCAGGCGAGGT+GGG - chr5.4:76203290-76203309 None:intergenic 70.0%
TCCGACACCCACCTCGCCTG+CGG + chr5.4:76203280-76203299 MS.gene020863:CDS 70.0%
CGACGAAGCCGCAGGCGAGG+TGG - chr5.4:76203291-76203310 None:intergenic 75.0%
CGTCGACGAAGCCGCAGGCG+AGG - chr5.4:76203294-76203313 None:intergenic 75.0%
GCCGCAGGCGAGGTGGGTGT+CGG - chr5.4:76203284-76203303 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 76203049 76204212 76203049 ID=MS.gene020863
chr5.4 mRNA 76203049 76204212 76203049 ID=MS.gene020863.t1;Parent=MS.gene020863
chr5.4 exon 76203049 76204212 76203049 ID=MS.gene020863.t1.exon1;Parent=MS.gene020863.t1
chr5.4 CDS 76203049 76204212 76203049 ID=cds.MS.gene020863.t1;Parent=MS.gene020863.t1
Gene Sequence

>MS.gene020863

ATGCAGGTTTGTTCAACATGGAGAGAAGTGTCACGTTCGGATCTACTGTGGCAGAGGCTCACGAGACGCATCTGGCGCAGAACATATAGATTGAGGGACACGTGGCAGTTAGAGTACATCTACTGGCATCGAACGGCCAGGAACTTTACCACCGGGAGACATGCTTTAGTGGTCCCACAATATGATCCAGGAGATCACCACCAAAACCTCATCTGCCGCTGCCTCACTCTCTCCGACACCCACCTCGCCTGCGGCTTCGTCGACGGCAGTGTCCGACTCTTCGATCTCCACACCGGTGCACATGTTAGCACATTTTGGTCCAACCATGGTCTTCTATTCGGCCCGTTTTCACAATCCGTTTCGGGAATTGTAATTGGAAGCTCTACCCTCGCCTTCGCGAGATTAGATGGAGATGTCTACATTGCTATTATTAATGGGCCTGGACCAATACCCGGGCCCATTCCTGCACGACGGGCAATTAATGGTGATGTTGTCAATAACGGAGTATTAGTAGAATTTGCCGGCTCTAGTCGTTGGTGGGTTGGTTTATATGTTGGCCACGCGGGCGGGGCTTTTCAAATATGGGACGCGCACACGGAACAGCGCGTGTTTGTTGGCGGTTCATTAACCGATCCGGAAACGGTTCAAGGATGGCATATGTTAACCGAATTAGTTGAACCGGTCGGTCGAGTCCGAGTCACCGAAAGAGAATTTGTAGTAGCTTGCACAAGTACAAGATTGGTTTGTTTCAACTTAAGAAACCCAGAGATTCTATTACGTGATGTAGTGTCCATAACAGGATTCATTGTAGGATCATTGGATGTTAGCCATGAGGTTTTTGTTGTGGTGGAAAGAAGTGGTGTTGGAACTGTTCGGCGCGTGAATAATTTCGAGCGCGTGAGTAGGTTTAGATTAAGGGCTTCATGGTTAAGAGGTTTATTAGGGTGCATGAATTTAGGGTATGTTATAACCTATTCTGGTGGGTCTGGTTTGTTAAGAGTGTGGGACATACATGAACCTGCGGCGCGATTGTGTATCACGCTCCCAGTTAGAGATGATGGAGAAGGACAAGTACATGGCAATAGCATGGTTGCTAATCAAACTCACGTGGCTATCTCATTTAATGACACTTCTATACGTTTATTGGATTTTAGTGTACAGTAA
Protein sequence

>MS.gene020863.t1

MQVCSTWREVSRSDLLWQRLTRRIWRRTYRLRDTWQLEYIYWHRTARNFTTGRHALVVPQYDPGDHHQNLICRCLTLSDTHLACGFVDGSVRLFDLHTGAHVSTFWSNHGLLFGPFSQSVSGIVIGSSTLAFARLDGDVYIAIINGPGPIPGPIPARRAINGDVVNNGVLVEFAGSSRWWVGLYVGHAGGAFQIWDAHTEQRVFVGGSLTDPETVQGWHMLTELVEPVGRVRVTEREFVVACTSTRLVCFNLRNPEILLRDVVSITGFIVGSLDVSHEVFVVVERSGVGTVRRVNNFERVSRFRLRASWLRGLLGCMNLGYVITYSGGSGLLRVWDIHEPAARLCITLPVRDDGEGQVHGNSMVANQTHVAISFNDTSIRLLDFSVQ