AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020898

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020898.t1 MTR_5g097480 99.081 544 5 0 1 544 1 544 0.0 1131
MS.gene020898.t1 MTR_3g056110 57.532 551 218 5 1 544 1 542 0.0 649
MS.gene020898.t1 MTR_5g094450 56.937 555 205 8 1 544 1 532 0.0 619
MS.gene020898.t1 MTR_4g133660 57.350 551 218 8 1 543 1 542 0.0 608
MS.gene020898.t1 MTR_2g082090 63.824 387 137 1 157 543 195 578 0.0 526
MS.gene020898.t1 MTR_2g026250 46.949 590 248 8 12 544 15 596 8.34e-179 517
MS.gene020898.t1 MTR_1g029420 56.216 370 162 0 175 544 223 592 3.40e-159 466
MS.gene020898.t1 MTR_3g089055 54.278 374 171 0 171 544 192 565 5.76e-151 444
MS.gene020898.t1 MTR_3g089055 54.278 374 171 0 171 544 197 570 6.90e-151 444
MS.gene020898.t1 MTR_7g027190 35.603 514 271 9 49 544 202 673 3.12e-87 283
MS.gene020898.t1 MTR_3g065980 36.923 390 209 7 174 544 169 540 2.53e-73 243
MS.gene020898.t1 MTR_7g062120 34.048 373 245 1 173 544 368 740 3.06e-69 237
MS.gene020898.t1 MTR_4g064160 32.796 372 249 1 173 543 312 683 2.82e-65 225
MS.gene020898.t1 MTR_8g442410 33.594 384 234 7 173 544 151 525 8.11e-65 220
MS.gene020898.t1 MTR_4g064150 32.447 376 249 3 173 543 357 732 1.08e-64 224
MS.gene020898.t1 MTR_4g064200 33.245 376 246 4 170 543 277 649 4.62e-64 221
MS.gene020898.t1 MTR_2g097390 29.150 494 310 10 74 543 163 640 1.19e-63 219
MS.gene020898.t1 MTR_7g057230 33.494 415 255 7 140 543 252 656 5.06e-63 218
MS.gene020898.t1 MTR_2g097310 28.279 488 317 9 74 543 165 637 8.15e-62 214
MS.gene020898.t1 MTR_3g022830 31.265 419 228 7 178 544 26 436 1.03e-60 207
MS.gene020898.t1 MTR_6g047750 35.354 396 204 13 175 544 293 662 1.89e-60 211
MS.gene020898.t1 MTR_4g076140 30.787 432 231 7 174 544 45 469 3.22e-60 206
MS.gene020898.t1 MTR_2g097350 31.200 375 249 4 173 543 270 639 5.74e-60 209
MS.gene020898.t1 MTR_4g104020 32.292 384 238 6 174 544 146 520 1.33e-59 206
MS.gene020898.t1 MTR_4g064120 29.904 418 282 4 129 541 212 623 1.57e-59 208
MS.gene020898.t1 MTR_2g097410 31.117 376 252 4 173 543 367 740 1.72e-59 210
MS.gene020898.t1 MTR_2g097467 31.095 402 270 4 143 543 258 653 3.32e-59 208
MS.gene020898.t1 MTR_4g064180 31.903 373 249 3 173 540 250 622 4.19e-59 207
MS.gene020898.t1 MTR_4g097080 30.890 382 243 7 175 544 131 503 4.80e-59 204
MS.gene020898.t1 MTR_5g009080 29.792 433 235 7 174 544 53 478 5.98e-58 201
MS.gene020898.t1 MTR_2g097473 32.976 373 244 5 173 543 285 653 6.91e-58 204
MS.gene020898.t1 MTR_7g074650 34.511 368 220 7 181 544 442 792 1.36e-57 206
MS.gene020898.t1 MTR_2g097463 31.720 372 250 3 173 543 286 654 8.74e-56 199
MS.gene020898.t1 MTR_4g102790 32.461 382 237 10 174 543 289 661 9.42e-52 187
MS.gene020898.t1 MTR_5g015490 29.592 392 253 8 169 544 106 490 9.48e-52 184
MS.gene020898.t1 MTR_2g097380 27.197 478 296 11 74 543 127 560 1.77e-49 179
MS.gene020898.t1 MTR_1g096030 31.778 450 254 16 127 544 31 459 1.94e-49 177
MS.gene020898.t1 MTR_4g077760 32.979 376 237 8 174 544 190 555 7.37e-47 172
MS.gene020898.t1 MTR_4g076020 33.062 369 215 9 181 544 88 429 2.03e-46 168
MS.gene020898.t1 MTR_5g058860 31.008 387 244 10 174 543 116 496 6.21e-44 163
MS.gene020898.t1 MTR_3g072710 29.426 401 250 10 167 543 108 499 4.03e-43 161
MS.gene020898.t1 MTR_2g089100 27.295 403 251 8 174 543 49 442 1.30e-41 155
MS.gene020898.t1 MTR_2g099110 28.974 390 243 9 177 544 74 451 3.72e-40 151
MS.gene020898.t1 MTR_1g069725 30.053 376 240 10 173 543 105 462 8.15e-40 151
MS.gene020898.t1 MTR_2g034250 25.974 385 257 10 178 544 211 585 1.75e-35 140
MS.gene020898.t1 MTR_4g095500 26.794 418 252 9 174 543 56 467 2.97e-34 135
MS.gene020898.t1 MTR_5g015950 25.806 434 238 12 174 543 32 445 3.27e-34 135
MS.gene020898.t1 MTR_3g053270 25.806 434 238 12 174 543 32 445 3.27e-34 135
MS.gene020898.t1 MTR_2g034260 25.882 340 229 8 178 503 211 541 2.02e-30 125
MS.gene020898.t1 MTR_2g034280 24.074 378 270 7 178 544 204 575 2.18e-30 125
MS.gene020898.t1 MTR_4g026485 24.595 370 261 8 178 544 270 624 2.27e-29 122
MS.gene020898.t1 MTR_0092s0100 23.662 355 254 7 193 544 391 731 3.54e-26 113
MS.gene020898.t1 MTR_0092s0100 23.662 355 254 7 193 544 388 728 3.71e-26 113
MS.gene020898.t1 MTR_7g109580 23.770 366 248 10 190 544 209 554 7.55e-26 111
MS.gene020898.t1 MTR_8g093070 28.186 408 249 19 154 543 114 495 1.10e-25 110
MS.gene020898.t1 MTR_1g086970 23.143 350 246 10 208 544 139 478 1.82e-20 94.7
MS.gene020898.t1 MTR_8g020840 25.185 405 243 16 175 544 169 548 5.02e-19 90.9
MS.gene020898.t1 MTR_3g022580 39.286 112 64 2 435 544 74 183 3.65e-18 83.2
MS.gene020898.t1 MTR_3g022005 43.158 95 50 2 452 544 33 125 1.53e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020898.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene020898.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene020898.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene020898.t1 AT5G48150 65.677 539 130 7 12 544 1 490 0.0 702
MS.gene020898.t1 AT1G50600 61.943 494 180 4 56 544 107 597 0.0 619
MS.gene020898.t1 AT1G50600 61.943 494 180 4 56 544 111 601 0.0 619
MS.gene020898.t1 AT1G50600 61.943 494 180 4 56 544 36 526 0.0 617
MS.gene020898.t1 AT2G04890 68.599 414 123 1 131 544 7 413 0.0 596
MS.gene020898.t1 AT4G17230 53.016 547 232 9 1 544 1 525 0.0 559
MS.gene020898.t1 AT4G17230 53.016 547 232 9 1 544 1 525 0.0 559
MS.gene020898.t1 AT1G21450 48.649 444 211 4 118 544 150 593 9.91e-151 445
MS.gene020898.t1 AT3G03450 36.800 375 224 7 174 544 180 545 1.47e-70 236
MS.gene020898.t1 AT1G66350 35.678 398 221 11 158 544 133 506 1.49e-70 235
MS.gene020898.t1 AT1G14920 36.061 391 225 9 163 544 155 529 3.20e-70 234
MS.gene020898.t1 AT2G01570 36.869 396 221 12 160 544 204 581 1.56e-68 231
MS.gene020898.t1 AT1G07530 33.333 375 248 2 171 543 390 764 3.30e-68 234
MS.gene020898.t1 AT5G52510 34.286 385 228 7 175 544 266 640 2.62e-66 227
MS.gene020898.t1 AT5G17490 34.933 375 225 9 174 544 157 516 1.48e-65 222
MS.gene020898.t1 AT2G29060 32.533 375 249 3 173 543 280 654 1.18e-64 223
MS.gene020898.t1 AT5G59450 31.383 376 254 3 173 544 223 598 1.50e-64 221
MS.gene020898.t1 AT2G29060 32.095 377 252 2 171 543 315 691 2.02e-64 223
MS.gene020898.t1 AT2G37650 31.903 373 251 2 173 544 331 701 3.06e-61 214
MS.gene020898.t1 AT2G37650 31.903 373 251 2 173 544 343 713 3.80e-61 214
MS.gene020898.t1 AT3G46600 31.452 372 253 2 174 544 1 371 4.50e-61 206
MS.gene020898.t1 AT1G50420 29.792 433 235 7 174 544 54 479 1.01e-60 208
MS.gene020898.t1 AT3G46600 31.367 373 254 2 173 544 208 579 4.58e-60 209
MS.gene020898.t1 AT3G46600 31.367 373 254 2 173 544 176 547 6.81e-60 207
MS.gene020898.t1 AT2G29065 32.461 382 245 7 173 543 248 627 3.88e-56 199
MS.gene020898.t1 AT3G54220 34.605 367 222 7 181 543 297 649 3.97e-56 199
MS.gene020898.t1 AT1G07520 30.446 381 253 5 173 543 150 528 3.55e-55 194
MS.gene020898.t1 AT1G07520 30.446 381 253 5 173 543 150 528 3.55e-55 194
MS.gene020898.t1 AT1G07520 30.446 381 253 5 173 543 315 693 1.12e-54 196
MS.gene020898.t1 AT1G07520 30.446 381 253 5 173 543 314 692 1.23e-54 196
MS.gene020898.t1 AT5G66770 34.595 370 227 8 181 544 224 584 1.24e-51 186
MS.gene020898.t1 AT5G41920 32.718 379 231 9 173 544 39 400 2.77e-48 172
MS.gene020898.t1 AT3G50650 35.294 357 207 9 200 544 198 542 9.43e-48 174
MS.gene020898.t1 AT4G37650 29.104 402 247 12 170 544 139 529 1.40e-47 174
MS.gene020898.t1 AT1G55580 30.994 342 204 8 226 544 113 445 1.34e-44 164
MS.gene020898.t1 AT1G63100 32.665 349 217 6 174 515 249 586 5.45e-44 165
MS.gene020898.t1 AT1G63100 32.665 349 217 6 174 515 274 611 7.06e-44 165
MS.gene020898.t1 AT4G08250 28.079 406 270 9 147 543 84 476 2.32e-39 150
MS.gene020898.t1 AT3G49950 25.389 386 267 9 174 543 27 407 3.21e-31 125
MS.gene020898.t1 AT2G45160 25.532 376 231 13 191 544 291 639 5.68e-27 115
MS.gene020898.t1 AT3G60630 28.571 301 190 8 256 544 335 622 9.21e-25 108
MS.gene020898.t1 AT3G13840 27.749 382 247 12 175 544 146 510 3.38e-24 106
MS.gene020898.t1 AT4G36710 25.000 380 252 12 178 544 126 485 1.21e-20 95.5
MS.gene020898.t1 AT4G00150 23.098 368 253 11 182 544 212 554 3.46e-20 94.4
MS.gene020898.t1 AT5G67411 33.929 112 72 2 370 481 89 198 1.19e-11 64.7

Find 122 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCAGATGGTGTCGGTTTC+TGG 0.116927 5.4:+76690195 MS.gene020898:CDS
GTTTGCCCTTACTTCAAATT+TGG 0.195361 5.4:+76690363 MS.gene020898:CDS
ATTGGCTTCCTTGAGCAATT+TGG 0.205067 5.4:-76690455 None:intergenic
CAACAAGTGTCACGACTTTC+GGG 0.226734 5.4:-76690812 None:intergenic
CATTTCAAGCGAAGCGAAAT+TGG 0.230694 5.4:-76690049 None:intergenic
CCAATGGATTTCGTTGATTC+AGG 0.238320 5.4:+76690473 MS.gene020898:CDS
TCAACAAGTGTCACGACTTT+CGG 0.277772 5.4:-76690813 None:intergenic
TCCTTACCCTCTGAGTTCTT+TGG 0.281649 5.4:+76691082 MS.gene020898:CDS
CAATTTCGCTTCGCTTGAAA+TGG 0.314726 5.4:+76690050 MS.gene020898:CDS
CAAGCACAAGCTTAGAGAAT+TGG 0.336532 5.4:+76689960 MS.gene020898:CDS
CAATGTTGAGCAGCATTGTT+TGG 0.339609 5.4:+76690956 MS.gene020898:CDS
CCACCACACATCCGGATTAC+AGG 0.342966 5.4:+76690519 MS.gene020898:CDS
TAGCATGAATGCGAAATTTA+CGG 0.352242 5.4:-76690715 None:intergenic
GTGTCAGCACTCAGAATCAT+AGG 0.362236 5.4:+76690757 MS.gene020898:CDS
ATCATTGTCTGCAATTGCTT+TGG 0.368256 5.4:-76690139 None:intergenic
TCGATATTGCGGCAAGCAAT+AGG 0.368619 5.4:-76689659 None:intergenic
AATGGTTGATGGACGAATTA+CGG 0.368703 5.4:+76690175 MS.gene020898:CDS
TGGTGAACCGATTCAAAGAT+TGG 0.393469 5.4:+76690215 MS.gene020898:CDS
CATAACCTTGCGGTTCGGCC+AGG 0.394232 5.4:+76690681 MS.gene020898:CDS
CTTGAAGCTACTTAAATCTT+CGG 0.395656 5.4:-76689941 None:intergenic
CCGGATGTGTGGTGGTCCCC+CGG 0.395807 5.4:-76690511 None:intergenic
CCTCTGAGTTCTTTGGTGAA+TGG 0.405916 5.4:+76691089 MS.gene020898:CDS
GTTGCACGGTTGTCTGCATC+CGG 0.415709 5.4:+76690261 MS.gene020898:CDS
TGCAGACAATGATTTGCTAA+TGG 0.419025 5.4:+76690149 MS.gene020898:CDS
ATTTGGTTGCTTCTTGTGCT+TGG 0.429491 5.4:+76691201 MS.gene020898:CDS
ATGTAACCAAATTTGAAGTA+AGG 0.431720 5.4:-76690369 None:intergenic
TTTGGTTACATGTCTGCAAA+TGG 0.433675 5.4:+76690381 MS.gene020898:CDS
GTTATGCACGATCCACTCAT+TGG 0.435852 5.4:-76689919 None:intergenic
GCTAATGGCGCAATGGTTGA+TGG 0.437770 5.4:+76690164 MS.gene020898:CDS
TGAGATTCTTGCTGCGACAT+TGG 0.437774 5.4:-76689847 None:intergenic
TTTCACGCTGCAGCTATCTC+TGG 0.438144 5.4:+76690642 MS.gene020898:CDS
AAAGCTTGCTCGGCATTTCA+AGG 0.448893 5.4:+76690608 MS.gene020898:CDS
AGATTTGTAGATTGAACTCC+CGG 0.451937 5.4:-76690280 None:intergenic
GGCGAGCCATAGGTATTGTC+GGG 0.452043 5.4:-76689898 None:intergenic
ACTCCAACGTGCAGTAATAA+AGG 0.452114 5.4:-76689753 None:intergenic
TTGGAGTCGTCCTTGGCAGC+TGG 0.456230 5.4:+76689769 MS.gene020898:CDS
TGGTTGGATGAATAGAGATT+TGG 0.456807 5.4:+76691184 MS.gene020898:CDS
AACTGCATAACCTTGCGGTT+CGG 0.459814 5.4:+76690676 MS.gene020898:CDS
TCAGCTTATGCCCGTGGAGG+TGG 0.460311 5.4:+76690558 MS.gene020898:CDS
ATCCTCTCTTTATGCTCTCT+CGG 0.463692 5.4:-76690933 None:intergenic
AGGTCAAGATTTGCAATGGC+TGG 0.465717 5.4:+76691053 MS.gene020898:CDS
TGGCGAGCCATAGGTATTGT+CGG 0.470434 5.4:-76689899 None:intergenic
GAGAGAAGCTCGGCACTCTC+TGG 0.471288 5.4:-76690318 None:intergenic
TATGCTCCCAATCTTTGAAT+CGG 0.477205 5.4:-76690222 None:intergenic
AGAGATGGAGCTCTATATCT+TGG 0.480538 5.4:+76691164 MS.gene020898:CDS
TAGGCTTCAATTTGCTGCAA+TGG 0.480682 5.4:-76689640 None:intergenic
TGAGTGCTGACACTCTCATC+TGG 0.480842 5.4:-76690747 None:intergenic
AGGCCAAGCAACTCAATTCT+CGG 0.481276 5.4:+76689714 MS.gene020898:CDS
GGTTCGGCCAGGGGAAGCTC+TGG 0.484150 5.4:+76690692 MS.gene020898:CDS
GGTGAACCGATTCAAAGATT+GGG 0.489961 5.4:+76690216 MS.gene020898:CDS
CTGCACGTTGGAGTCGTCCT+TGG 0.491134 5.4:+76689762 MS.gene020898:CDS
CTATCAAGATTATCAGAGTC+AGG 0.491387 5.4:-76690000 None:intergenic
ATAACCTTGCGGTTCGGCCA+GGG 0.491957 5.4:+76690682 MS.gene020898:CDS
GAAATCGATTATATGAACTC+TGG 0.496688 5.4:-76690433 None:intergenic
GAGCCGAGAATTGAGTTGCT+TGG 0.498447 5.4:-76689717 None:intergenic
TAATAAAGGTCAGAAGAAGT+TGG 0.499526 5.4:-76689739 None:intergenic
CATCAGCTGTACAACAATGA+TGG 0.501721 5.4:+76689691 MS.gene020898:CDS
AACAAAATTTCCAGCTGCCA+AGG 0.505944 5.4:-76689779 None:intergenic
CTTCGCTTGAAATGGACAGT+TGG 0.507924 5.4:+76690058 MS.gene020898:CDS
ACCAAAGAACTCAGAGGGTA+AGG 0.509605 5.4:-76691083 None:intergenic
GATCATCAATACCTGTAATC+CGG 0.510867 5.4:-76690530 None:intergenic
GACACGAGCTGCTAGGGAAG+TGG 0.514693 5.4:+76691030 MS.gene020898:CDS
GTGTCACGACTTTCGGGGAT+AGG 0.518113 5.4:-76690806 None:intergenic
TCCACTCATTGGCGAGCCAT+AGG 0.519637 5.4:-76689908 None:intergenic
TGGAACGACACGAGCTGCTA+GGG 0.521228 5.4:+76691024 MS.gene020898:CDS
GTGGAACGACACGAGCTGCT+AGG 0.524981 5.4:+76691023 MS.gene020898:CDS
ATGGAGCTCTATATCTTGGT+TGG 0.526117 5.4:+76691168 MS.gene020898:CDS
TTGCTTTGGCACATGCAGTA+AGG 0.529396 5.4:-76690125 None:intergenic
GTTAACATCATAGCTTGTGA+AGG 0.534917 5.4:+76690990 MS.gene020898:CDS
CGAATTACGGCAGATGGTGT+CGG 0.542149 5.4:+76690188 MS.gene020898:CDS
TGATTTAGATTTCGATATTG+CGG 0.543411 5.4:-76689670 None:intergenic
GCTGCAGCGTGAAACTCAAA+TGG 0.545283 5.4:-76690633 None:intergenic
TGTAACCAAATTTGAAGTAA+GGG 0.545514 5.4:-76690368 None:intergenic
TAACCTTGCGGTTCGGCCAG+GGG 0.545938 5.4:+76690683 MS.gene020898:CDS
ACCTATGGCTCGCCAATGAG+TGG 0.546193 5.4:+76689907 MS.gene020898:CDS
ATCGATTTCCAAATTGCTCA+AGG 0.546444 5.4:+76690447 MS.gene020898:CDS
GCACTCAGAATCATAGGGAT+AGG 0.551891 5.4:+76690763 MS.gene020898:CDS
TTAACATCATAGCTTGTGAA+GGG 0.552742 5.4:+76690991 MS.gene020898:CDS
CCTGAATCAACGAAATCCAT+TGG 0.554910 5.4:-76690473 None:intergenic
GGGGAATGATACTTATCAGA+TGG 0.562828 5.4:-76689877 None:intergenic
TGCGACATTGGACTATCGTT+AGG 0.563587 5.4:-76689835 None:intergenic
TTTACGGCCAGAGCTTCCCC+TGG 0.565827 5.4:-76690699 None:intergenic
GAATTGGAAAATGTAATGCT+TGG 0.570289 5.4:+76689976 MS.gene020898:CDS
CTTCCCCTGGCCGAACCGCA+AGG 0.580073 5.4:-76690686 None:intergenic
TTGCACGGTTGTCTGCATCC+GGG 0.585418 5.4:+76690262 MS.gene020898:CDS
TGTTGGAAGGACTCGTTGCA+CGG 0.585796 5.4:+76690247 MS.gene020898:CDS
TATATGTAGTCCACCTCCAC+GGG 0.589655 5.4:-76690568 None:intergenic
TATAGACTTGAAGAGAGAGA+TGG 0.590094 5.4:+76691149 MS.gene020898:CDS
TTGTGAAGGGGTCGAGAGAG+TGG 0.594827 5.4:+76691004 MS.gene020898:CDS
GACTACATATAGTTGAAAAG+AGG 0.595404 5.4:+76690580 MS.gene020898:CDS
TGTCAGCACTCAGAATCATA+GGG 0.595636 5.4:+76690758 MS.gene020898:CDS
AGAGGTTATCAAAGCTTGCT+CGG 0.596778 5.4:+76690598 MS.gene020898:CDS
AAGATTGGGAGCATACATGT+TGG 0.605912 5.4:+76690230 MS.gene020898:CDS
CTATATGTAGTCCACCTCCA+CGG 0.608482 5.4:-76690569 None:intergenic
TCCATAGTTTCGAGAAAACG+TGG 0.611195 5.4:-76690870 None:intergenic
GTGGAGGTCAAGATTTGCAA+TGG 0.615832 5.4:+76691049 MS.gene020898:CDS
TTGGGAGCATACATGTTGGA+AGG 0.617711 5.4:+76690234 MS.gene020898:CDS
TACGACAGTGCTATCAGCAA+CGG 0.617830 5.4:+76690024 MS.gene020898:CDS
TGACCTTTATTACTGCACGT+TGG 0.620270 5.4:+76689750 MS.gene020898:CDS
GGCGAGAAACTAACAGTCGA+AGG 0.623572 5.4:-76689814 None:intergenic
AAATTGCTCAAGGAAGCCAA+TGG 0.626215 5.4:+76690457 MS.gene020898:CDS
GATGGACGAATTACGGCAGA+TGG 0.627072 5.4:+76690182 MS.gene020898:CDS
TTACATGAACATACTTTACG+AGG 0.627108 5.4:+76690341 MS.gene020898:CDS
ACGAGCTGCTAGGGAAGTGG+AGG 0.634312 5.4:+76691033 MS.gene020898:CDS
CCACGGGCATAAGCTGATGT+CGG 0.634703 5.4:-76690552 None:intergenic
CATTCACCAAAGAACTCAGA+GGG 0.637813 5.4:-76691088 None:intergenic
CCGACATCAGCTTATGCCCG+TGG 0.638695 5.4:+76690552 MS.gene020898:CDS
ATGATTTGCTAATGGCGCAA+TGG 0.646542 5.4:+76690157 MS.gene020898:CDS
TTCCGAGAGAGCATAAAGAG+AGG 0.651166 5.4:+76690931 MS.gene020898:CDS
GCGAGCCATAGGTATTGTCG+GGG 0.657718 5.4:-76689897 None:intergenic
CAGCTGTACAACAATGATGG+AGG 0.659045 5.4:+76689694 MS.gene020898:CDS
TGTTCAACTGCATAACCTTG+CGG 0.660074 5.4:+76690671 MS.gene020898:CDS
ACATCAGCTTATGCCCGTGG+AGG 0.663564 5.4:+76690555 MS.gene020898:CDS
TAACATCATAGCTTGTGAAG+GGG 0.668817 5.4:+76690992 MS.gene020898:CDS
CCTGTAATCCGGATGTGTGG+TGG 0.675147 5.4:-76690519 None:intergenic
GGACAGTTGGAGACAAACAA+TGG 0.681512 5.4:+76690071 MS.gene020898:CDS
AACAAGTGTCACGACTTTCG+GGG 0.682943 5.4:-76690811 None:intergenic
GTTCATGTAAGAGAGAAGCT+CGG 0.686963 5.4:-76690328 None:intergenic
CCATTCACCAAAGAACTCAG+AGG 0.710837 5.4:-76691089 None:intergenic
ATACCTGTAATCCGGATGTG+TGG 0.719840 5.4:-76690522 None:intergenic
CGAGCCATAGGTATTGTCGG+GGG 0.727707 5.4:-76689896 None:intergenic
AGAATCTAACACAAACACTG+CGG 0.784454 5.4:+76690839 MS.gene020898:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATTTAGATTTCGATATTG+CGG - chr5.4:76689673-76689692 None:intergenic 25.0%
!! ATGTAACCAAATTTGAAGTA+AGG - chr5.4:76690372-76690391 None:intergenic 25.0%
!! TGTAACCAAATTTGAAGTAA+GGG - chr5.4:76690371-76690390 None:intergenic 25.0%
!!! GTTTTCAAGCAATTTCTTTA+TGG - chr5.4:76691117-76691136 None:intergenic 25.0%
CTTGAAGCTACTTAAATCTT+CGG - chr5.4:76689944-76689963 None:intergenic 30.0%
GAAATCGATTATATGAACTC+TGG - chr5.4:76690436-76690455 None:intergenic 30.0%
GAATTGGAAAATGTAATGCT+TGG + chr5.4:76689976-76689995 MS.gene020898:CDS 30.0%
GACTACATATAGTTGAAAAG+AGG + chr5.4:76690580-76690599 MS.gene020898:CDS 30.0%
TAATAAAGGTCAGAAGAAGT+TGG - chr5.4:76689742-76689761 None:intergenic 30.0%
TAGCATGAATGCGAAATTTA+CGG - chr5.4:76690718-76690737 None:intergenic 30.0%
TTACATGAACATACTTTACG+AGG + chr5.4:76690341-76690360 MS.gene020898:CDS 30.0%
! TTAACATCATAGCTTGTGAA+GGG + chr5.4:76690991-76691010 MS.gene020898:CDS 30.0%
AGAATCTAACACAAACACTG+CGG + chr5.4:76690839-76690858 MS.gene020898:CDS 35.0%
AGATTTGTAGATTGAACTCC+CGG - chr5.4:76690283-76690302 None:intergenic 35.0%
ATCATTGTCTGCAATTGCTT+TGG - chr5.4:76690142-76690161 None:intergenic 35.0%
CTATCAAGATTATCAGAGTC+AGG - chr5.4:76690003-76690022 None:intergenic 35.0%
GATCATCAATACCTGTAATC+CGG - chr5.4:76690533-76690552 None:intergenic 35.0%
GTTTGCCCTTACTTCAAATT+TGG + chr5.4:76690363-76690382 MS.gene020898:CDS 35.0%
TATAGACTTGAAGAGAGAGA+TGG + chr5.4:76691149-76691168 MS.gene020898:CDS 35.0%
TATGCTCCCAATCTTTGAAT+CGG - chr5.4:76690225-76690244 None:intergenic 35.0%
TGGTTGGATGAATAGAGATT+TGG + chr5.4:76691184-76691203 MS.gene020898:CDS 35.0%
TTTGGTTACATGTCTGCAAA+TGG + chr5.4:76690381-76690400 MS.gene020898:CDS 35.0%
! ATCATAGGGATAGGCTTTTA+AGG + chr5.4:76690772-76690791 MS.gene020898:CDS 35.0%
! ATCGATTTCCAAATTGCTCA+AGG + chr5.4:76690447-76690466 MS.gene020898:CDS 35.0%
! ATTTTTCATGGCTTCTGCAA+TGG - chr5.4:76690409-76690428 None:intergenic 35.0%
! GTTAACATCATAGCTTGTGA+AGG + chr5.4:76690990-76691009 MS.gene020898:CDS 35.0%
! TAACATCATAGCTTGTGAAG+GGG + chr5.4:76690992-76691011 MS.gene020898:CDS 35.0%
!! AACTCTGGCTTCATTTTTCA+TGG - chr5.4:76690421-76690440 None:intergenic 35.0%
!! AATGGTTGATGGACGAATTA+CGG + chr5.4:76690175-76690194 MS.gene020898:CDS 35.0%
!! TGCAGACAATGATTTGCTAA+TGG + chr5.4:76690149-76690168 MS.gene020898:CDS 35.0%
AAATTGCTCAAGGAAGCCAA+TGG + chr5.4:76690457-76690476 MS.gene020898:CDS 40.0%
AACAAAATTTCCAGCTGCCA+AGG - chr5.4:76689782-76689801 None:intergenic 40.0%
ACTCCAACGTGCAGTAATAA+AGG - chr5.4:76689756-76689775 None:intergenic 40.0%
AGAGATGGAGCTCTATATCT+TGG + chr5.4:76691164-76691183 MS.gene020898:CDS 40.0%
ATCCTCTCTTTATGCTCTCT+CGG - chr5.4:76690936-76690955 None:intergenic 40.0%
ATGGAGCTCTATATCTTGGT+TGG + chr5.4:76691168-76691187 MS.gene020898:CDS 40.0%
ATTTGGTTGCTTCTTGTGCT+TGG + chr5.4:76691201-76691220 MS.gene020898:CDS 40.0%
CAAGCACAAGCTTAGAGAAT+TGG + chr5.4:76689960-76689979 MS.gene020898:CDS 40.0%
CAATGTTGAGCAGCATTGTT+TGG + chr5.4:76690956-76690975 MS.gene020898:CDS 40.0%
CAATTTCGCTTCGCTTGAAA+TGG + chr5.4:76690050-76690069 MS.gene020898:CDS 40.0%
CATCAGCTGTACAACAATGA+TGG + chr5.4:76689691-76689710 MS.gene020898:CDS 40.0%
CATTCACCAAAGAACTCAGA+GGG - chr5.4:76691091-76691110 None:intergenic 40.0%
CATTTCAAGCGAAGCGAAAT+TGG - chr5.4:76690052-76690071 None:intergenic 40.0%
CCTGAATCAACGAAATCCAT+TGG - chr5.4:76690476-76690495 None:intergenic 40.0%
GGGGAATGATACTTATCAGA+TGG - chr5.4:76689880-76689899 None:intergenic 40.0%
GTTCATGTAAGAGAGAAGCT+CGG - chr5.4:76690331-76690350 None:intergenic 40.0%
TCAACAAGTGTCACGACTTT+CGG - chr5.4:76690816-76690835 None:intergenic 40.0%
TCCATAGTTTCGAGAAAACG+TGG - chr5.4:76690873-76690892 None:intergenic 40.0%
TGACCTTTATTACTGCACGT+TGG + chr5.4:76689750-76689769 MS.gene020898:CDS 40.0%
TGTCAGCACTCAGAATCATA+GGG + chr5.4:76690758-76690777 MS.gene020898:CDS 40.0%
TGTTCAACTGCATAACCTTG+CGG + chr5.4:76690671-76690690 MS.gene020898:CDS 40.0%
! AAGATTGGGAGCATACATGT+TGG + chr5.4:76690230-76690249 MS.gene020898:CDS 40.0%
! AGAGGTTATCAAAGCTTGCT+CGG + chr5.4:76690598-76690617 MS.gene020898:CDS 40.0%
! ATTGGCTTCCTTGAGCAATT+TGG - chr5.4:76690458-76690477 None:intergenic 40.0%
! GGTGAACCGATTCAAAGATT+GGG + chr5.4:76690216-76690235 MS.gene020898:CDS 40.0%
! TCCACGTTTTCTCGAAACTA+TGG + chr5.4:76690869-76690888 MS.gene020898:CDS 40.0%
! TGGTGAACCGATTCAAAGAT+TGG + chr5.4:76690215-76690234 MS.gene020898:CDS 40.0%
!! ATGATTTGCTAATGGCGCAA+TGG + chr5.4:76690157-76690176 MS.gene020898:CDS 40.0%
!! CCAATGGATTTCGTTGATTC+AGG + chr5.4:76690473-76690492 MS.gene020898:CDS 40.0%
!! TAGGCTTCAATTTGCTGCAA+TGG - chr5.4:76689643-76689662 None:intergenic 40.0%
!!! TAGGGATAGGCTTTTAAGGT+TGG + chr5.4:76690776-76690795 MS.gene020898:CDS 40.0%
AACAAGTGTCACGACTTTCG+GGG - chr5.4:76690814-76690833 None:intergenic 45.0%
AACTGCATAACCTTGCGGTT+CGG + chr5.4:76690676-76690695 MS.gene020898:CDS 45.0%
ACCAAAGAACTCAGAGGGTA+AGG - chr5.4:76691086-76691105 None:intergenic 45.0%
AGGCCAAGCAACTCAATTCT+CGG + chr5.4:76689714-76689733 MS.gene020898:CDS 45.0%
AGGTCAAGATTTGCAATGGC+TGG + chr5.4:76691053-76691072 MS.gene020898:CDS 45.0%
ATACCTGTAATCCGGATGTG+TGG - chr5.4:76690525-76690544 None:intergenic 45.0%
CAACAAGTGTCACGACTTTC+GGG - chr5.4:76690815-76690834 None:intergenic 45.0%
CAGCTGTACAACAATGATGG+AGG + chr5.4:76689694-76689713 MS.gene020898:CDS 45.0%
CCATTCACCAAAGAACTCAG+AGG - chr5.4:76691092-76691111 None:intergenic 45.0%
CCTCTGAGTTCTTTGGTGAA+TGG + chr5.4:76691089-76691108 MS.gene020898:CDS 45.0%
CTATATGTAGTCCACCTCCA+CGG - chr5.4:76690572-76690591 None:intergenic 45.0%
CTTCGCTTGAAATGGACAGT+TGG + chr5.4:76690058-76690077 MS.gene020898:CDS 45.0%
GCACTCAGAATCATAGGGAT+AGG + chr5.4:76690763-76690782 MS.gene020898:CDS 45.0%
GGACAGTTGGAGACAAACAA+TGG + chr5.4:76690071-76690090 MS.gene020898:CDS 45.0%
GTGGAGGTCAAGATTTGCAA+TGG + chr5.4:76691049-76691068 MS.gene020898:CDS 45.0%
GTGTCAGCACTCAGAATCAT+AGG + chr5.4:76690757-76690776 MS.gene020898:CDS 45.0%
GTTATGCACGATCCACTCAT+TGG - chr5.4:76689922-76689941 None:intergenic 45.0%
TACGACAGTGCTATCAGCAA+CGG + chr5.4:76690024-76690043 MS.gene020898:CDS 45.0%
TATATGTAGTCCACCTCCAC+GGG - chr5.4:76690571-76690590 None:intergenic 45.0%
TCCTTACCCTCTGAGTTCTT+TGG + chr5.4:76691082-76691101 MS.gene020898:CDS 45.0%
TCGATATTGCGGCAAGCAAT+AGG - chr5.4:76689662-76689681 None:intergenic 45.0%
TGAGATTCTTGCTGCGACAT+TGG - chr5.4:76689850-76689869 None:intergenic 45.0%
TGCGACATTGGACTATCGTT+AGG - chr5.4:76689838-76689857 None:intergenic 45.0%
TTGCTTTGGCACATGCAGTA+AGG - chr5.4:76690128-76690147 None:intergenic 45.0%
! AAAGCTTGCTCGGCATTTCA+AGG + chr5.4:76690608-76690627 MS.gene020898:CDS 45.0%
! TGATTCAGGCTTTTGCAGCT+AGG + chr5.4:76690487-76690506 MS.gene020898:CDS 45.0%
! TTCCGAGAGAGCATAAAGAG+AGG + chr5.4:76690931-76690950 MS.gene020898:CDS 45.0%
! TTGGGAGCATACATGTTGGA+AGG + chr5.4:76690234-76690253 MS.gene020898:CDS 45.0%
CATTCCCCCGACAATACCTA+TGG + chr5.4:76689892-76689911 MS.gene020898:CDS 50.0%
CGAATTACGGCAGATGGTGT+CGG + chr5.4:76690188-76690207 MS.gene020898:CDS 50.0%
GATGGACGAATTACGGCAGA+TGG + chr5.4:76690182-76690201 MS.gene020898:CDS 50.0%
GCTAATGGCGCAATGGTTGA+TGG + chr5.4:76690164-76690183 MS.gene020898:CDS 50.0%
GCTGCAGCGTGAAACTCAAA+TGG - chr5.4:76690636-76690655 None:intergenic 50.0%
GGCGAGAAACTAACAGTCGA+AGG - chr5.4:76689817-76689836 None:intergenic 50.0%
TGTTGGAAGGACTCGTTGCA+CGG + chr5.4:76690247-76690266 MS.gene020898:CDS 50.0%
TTTCACGCTGCAGCTATCTC+TGG + chr5.4:76690642-76690661 MS.gene020898:CDS 50.0%
! GAGCCGAGAATTGAGTTGCT+TGG - chr5.4:76689720-76689739 None:intergenic 50.0%
! TGGCGAGCCATAGGTATTGT+CGG - chr5.4:76689902-76689921 None:intergenic 50.0%
!! TGAGTGCTGACACTCTCATC+TGG - chr5.4:76690750-76690769 None:intergenic 50.0%
ACATCAGCTTATGCCCGTGG+AGG + chr5.4:76690555-76690574 MS.gene020898:CDS 55.0%
ACCTATGGCTCGCCAATGAG+TGG + chr5.4:76689907-76689926 MS.gene020898:CDS 55.0%
CCACCACACATCCGGATTAC+AGG + chr5.4:76690519-76690538 MS.gene020898:CDS 55.0%
CCACGGGCATAAGCTGATGT+CGG - chr5.4:76690555-76690574 None:intergenic 55.0%
CCTGTAATCCGGATGTGTGG+TGG - chr5.4:76690522-76690541 None:intergenic 55.0%
GTGTCACGACTTTCGGGGAT+AGG - chr5.4:76690809-76690828 None:intergenic 55.0%
GTTGCACGGTTGTCTGCATC+CGG + chr5.4:76690261-76690280 MS.gene020898:CDS 55.0%
TCCACTCATTGGCGAGCCAT+AGG - chr5.4:76689911-76689930 None:intergenic 55.0%
TGGAACGACACGAGCTGCTA+GGG + chr5.4:76691024-76691043 MS.gene020898:CDS 55.0%
TTGCACGGTTGTCTGCATCC+GGG + chr5.4:76690262-76690281 MS.gene020898:CDS 55.0%
TTGTGAAGGGGTCGAGAGAG+TGG + chr5.4:76691004-76691023 MS.gene020898:CDS 55.0%
! ATAACCTTGCGGTTCGGCCA+GGG + chr5.4:76690682-76690701 MS.gene020898:CDS 55.0%
! CGAGCCATAGGTATTGTCGG+GGG - chr5.4:76689899-76689918 None:intergenic 55.0%
! GCGAGCCATAGGTATTGTCG+GGG - chr5.4:76689900-76689919 None:intergenic 55.0%
! GGCGAGCCATAGGTATTGTC+GGG - chr5.4:76689901-76689920 None:intergenic 55.0%
ACGAGCTGCTAGGGAAGTGG+AGG + chr5.4:76691033-76691052 MS.gene020898:CDS 60.0%
CCGACATCAGCTTATGCCCG+TGG + chr5.4:76690552-76690571 MS.gene020898:CDS 60.0%
CTGCACGTTGGAGTCGTCCT+TGG + chr5.4:76689762-76689781 MS.gene020898:CDS 60.0%
GACACGAGCTGCTAGGGAAG+TGG + chr5.4:76691030-76691049 MS.gene020898:CDS 60.0%
GAGAGAAGCTCGGCACTCTC+TGG - chr5.4:76690321-76690340 None:intergenic 60.0%
GTGGAACGACACGAGCTGCT+AGG + chr5.4:76691023-76691042 MS.gene020898:CDS 60.0%
TCAGCTTATGCCCGTGGAGG+TGG + chr5.4:76690558-76690577 MS.gene020898:CDS 60.0%
TTGGAGTCGTCCTTGGCAGC+TGG + chr5.4:76689769-76689788 MS.gene020898:CDS 60.0%
TTTACGGCCAGAGCTTCCCC+TGG - chr5.4:76690702-76690721 None:intergenic 60.0%
! AGGCTTTTGCAGCTAGGCCC+GGG + chr5.4:76690493-76690512 MS.gene020898:CDS 60.0%
! CAGGCTTTTGCAGCTAGGCC+CGG + chr5.4:76690492-76690511 MS.gene020898:CDS 60.0%
! CATAACCTTGCGGTTCGGCC+AGG + chr5.4:76690681-76690700 MS.gene020898:CDS 60.0%
! TAACCTTGCGGTTCGGCCAG+GGG + chr5.4:76690683-76690702 MS.gene020898:CDS 60.0%
!! CGGCAGATGGTGTCGGTTTC+TGG + chr5.4:76690195-76690214 MS.gene020898:CDS 60.0%
! GCTTTTGCAGCTAGGCCCGG+GGG + chr5.4:76690495-76690514 MS.gene020898:CDS 65.0%
! GGCTTTTGCAGCTAGGCCCG+GGG + chr5.4:76690494-76690513 MS.gene020898:CDS 65.0%
CCGGATGTGTGGTGGTCCCC+CGG - chr5.4:76690514-76690533 None:intergenic 70.0%
CCGGGGGACCACCACACATC+CGG + chr5.4:76690511-76690530 MS.gene020898:CDS 70.0%
CGGATGTGTGGTGGTCCCCC+GGG - chr5.4:76690513-76690532 None:intergenic 70.0%
CTTCCCCTGGCCGAACCGCA+AGG - chr5.4:76690689-76690708 None:intergenic 70.0%
GGTTCGGCCAGGGGAAGCTC+TGG + chr5.4:76690692-76690711 MS.gene020898:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 76689595 76691229 76689595 ID=MS.gene020898
chr5.4 mRNA 76689595 76691229 76689595 ID=MS.gene020898.t1;Parent=MS.gene020898
chr5.4 exon 76689595 76691229 76689595 ID=MS.gene020898.t1.exon1;Parent=MS.gene020898.t1
chr5.4 CDS 76689595 76691229 76689595 ID=cds.MS.gene020898.t1;Parent=MS.gene020898.t1
Gene Sequence

>MS.gene020898

ATGCAAGCATCAGAGCAGCATAGAAGTTCAAGTATGTACTATCAACCATTGCAGCAAATTGAAGCCTATTGCTTGCCGCAATATCGAAATCTAAATCATCAGCTGTACAACAATGATGGAGGCCAAGCAACTCAATTCTCGGCTCCAACTTCTTCTGACCTTTATTACTGCACGTTGGAGTCGTCCTTGGCAGCTGGAAATTTTGTTGTTTACAACTCTCCTTCGACTGTTAGTTTCTCGCCTAACGATAGTCCAATGTCGCAGCAAGAATCTCAGTCTTATCCATCTGATAAGTATCATTCCCCCGACAATACCTATGGCTCGCCAATGAGTGGATCGTGCATAACCGAAGATTTAAGTAGCTTCAAGCACAAGCTTAGAGAATTGGAAAATGTAATGCTTGGTCCTGACTCTGATAATCTTGATAGTTACGACAGTGCTATCAGCAACGGAACCAATTTCGCTTCGCTTGAAATGGACAGTTGGAGACAAACAATGGTAGCAATTTCGAGCAAAAATCTAAAGCACATCCTTACTGCATGTGCCAAAGCAATTGCAGACAATGATTTGCTAATGGCGCAATGGTTGATGGACGAATTACGGCAGATGGTGTCGGTTTCTGGTGAACCGATTCAAAGATTGGGAGCATACATGTTGGAAGGACTCGTTGCACGGTTGTCTGCATCCGGGAGTTCAATCTACAAATCTTTAAGATGCAAAGAACCAGAGAGTGCCGAGCTTCTCTCTTACATGAACATACTTTACGAGGTTTGCCCTTACTTCAAATTTGGTTACATGTCTGCAAATGGAGCCATTGCAGAAGCCATGAAAAATGAAGCCAGAGTTCATATAATCGATTTCCAAATTGCTCAAGGAAGCCAATGGATTTCGTTGATTCAGGCTTTTGCAGCTAGGCCCGGGGGACCACCACACATCCGGATTACAGGTATTGATGATCCGACATCAGCTTATGCCCGTGGAGGTGGACTACATATAGTTGAAAAGAGGTTATCAAAGCTTGCTCGGCATTTCAAGGTGCCATTTGAGTTTCACGCTGCAGCTATCTCTGGTTGTGATGTTCAACTGCATAACCTTGCGGTTCGGCCAGGGGAAGCTCTGGCCGTAAATTTCGCATTCATGCTACATCACATGCCAGATGAGAGTGTCAGCACTCAGAATCATAGGGATAGGCTTTTAAGGTTGGTTAAGAGCCTATCCCCGAAAGTCGTGACACTTGTTGAGCAAGAATCTAACACAAACACTGCGGCATTCTTTCCACGTTTTCTCGAAACTATGGACTATTACACAGCAATGTTCGAGTCAATAGACGTGACTCTTCCGAGAGAGCATAAAGAGAGGATCAATGTTGAGCAGCATTGTTTGGCAAGAGATTTAGTTAACATCATAGCTTGTGAAGGGGTCGAGAGAGTGGAACGACACGAGCTGCTAGGGAAGTGGAGGTCAAGATTTGCAATGGCTGGTTTCACTCCTTACCCTCTGAGTTCTTTGGTGAATGGTACCATAAAGAAATTGCTTGAAAACTACAGTGATAGATATAGACTTGAAGAGAGAGATGGAGCTCTATATCTTGGTTGGATGAATAGAGATTTGGTTGCTTCTTGTGCTTGGAAATGA
Protein sequence

>MS.gene020898.t1

MQASEQHRSSSMYYQPLQQIEAYCLPQYRNLNHQLYNNDGGQATQFSAPTSSDLYYCTLESSLAAGNFVVYNSPSTVSFSPNDSPMSQQESQSYPSDKYHSPDNTYGSPMSGSCITEDLSSFKHKLRELENVMLGPDSDNLDSYDSAISNGTNFASLEMDSWRQTMVAISSKNLKHILTACAKAIADNDLLMAQWLMDELRQMVSVSGEPIQRLGAYMLEGLVARLSASGSSIYKSLRCKEPESAELLSYMNILYEVCPYFKFGYMSANGAIAEAMKNEARVHIIDFQIAQGSQWISLIQAFAARPGGPPHIRITGIDDPTSAYARGGGLHIVEKRLSKLARHFKVPFEFHAAAISGCDVQLHNLAVRPGEALAVNFAFMLHHMPDESVSTQNHRDRLLRLVKSLSPKVVTLVEQESNTNTAAFFPRFLETMDYYTAMFESIDVTLPREHKERINVEQHCLARDLVNIIACEGVERVERHELLGKWRSRFAMAGFTPYPLSSLVNGTIKKLLENYSDRYRLEERDGALYLGWMNRDLVASCAWK