AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020902

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020902.t1 MTR_5g097500 82.958 311 24 1 1 282 1 311 0.0 531
MS.gene020902.t1 MTR_5g097490 51.524 328 108 3 1 277 1 328 8.63e-109 320
MS.gene020902.t1 MTR_4g021780 72.727 99 26 1 75 173 15 112 3.71e-49 159
MS.gene020902.t1 MTR_4g013260 34.667 225 111 5 77 265 176 400 6.62e-38 139
MS.gene020902.t1 MTR_7g071120 33.740 246 117 6 76 275 158 403 1.06e-37 139
MS.gene020902.t1 MTR_3g056100 32.068 237 120 1 75 270 91 327 2.70e-36 134
MS.gene020902.t1 MTR_5g094430 29.221 308 156 5 20 277 11 306 2.85e-36 134
MS.gene020902.t1 MTR_5g094430 29.221 308 156 5 20 277 33 328 3.42e-36 134
MS.gene020902.t1 MTR_5g094430 32.500 240 116 3 84 277 201 440 7.65e-36 134
MS.gene020902.t1 MTR_5g094430 41.463 82 45 2 180 260 179 258 4.26e-12 66.6
MS.gene020902.t1 MTR_3g056100 32.068 237 120 1 75 270 235 471 8.45e-36 135
MS.gene020902.t1 MTR_3g056100 31.200 250 118 3 75 270 91 340 1.20e-34 130
MS.gene020902.t1 MTR_3g056100 30.800 250 119 3 75 270 235 484 1.08e-33 129
MS.gene020902.t1 MTR_5g074400 31.667 240 105 4 84 264 244 483 1.46e-33 129
MS.gene020902.t1 MTR_4g082580 33.333 213 127 5 84 281 146 358 2.33e-33 125
MS.gene020902.t1 MTR_3g031220 30.870 230 110 4 84 264 199 428 3.01e-32 124
MS.gene020902.t1 MTR_7g100510 30.736 231 106 6 84 260 190 420 1.70e-26 107
MS.gene020902.t1 MTR_7g100510 45.588 68 36 1 193 260 181 247 9.72e-12 65.1
MS.gene020902.t1 MTR_7g080020 43.333 90 51 0 186 275 478 567 2.17e-20 91.3
MS.gene020902.t1 MTR_7g080020 45.205 73 37 1 83 152 280 352 1.80e-14 73.9
MS.gene020902.t1 MTR_3g009470 44.706 85 47 0 186 270 480 564 2.97e-20 90.9
MS.gene020902.t1 MTR_3g009470 47.458 59 31 0 83 141 280 338 1.50e-13 70.9
MS.gene020902.t1 MTR_5g042920 42.045 88 51 0 185 272 304 391 2.98e-20 90.1
MS.gene020902.t1 MTR_5g042920 46.667 60 30 1 84 141 152 211 1.31e-11 64.7
MS.gene020902.t1 MTR_5g042920 35.417 96 61 1 185 280 135 229 3.26e-11 63.5
MS.gene020902.t1 MTR_7g011820 40.909 88 52 0 185 272 394 481 1.25e-19 89.0
MS.gene020902.t1 MTR_7g011820 47.458 59 31 0 84 142 229 287 2.16e-13 70.5
MS.gene020902.t1 MTR_7g011820 46.774 62 30 2 200 260 227 286 4.12e-11 63.5
MS.gene020902.t1 MTR_7g080000 46.753 77 41 0 187 263 418 494 2.61e-19 88.2
MS.gene020902.t1 MTR_7g080000 45.161 62 34 0 83 144 234 295 2.07e-13 70.5
MS.gene020902.t1 MTR_3g031220 29.508 183 78 6 84 216 199 380 3.53e-17 80.9
MS.gene020902.t1 MTR_1g077780 36.585 82 52 0 186 267 47 128 1.17e-15 72.8
MS.gene020902.t1 MTR_7g079010 36.264 91 58 0 167 257 107 197 1.58e-15 74.3
MS.gene020902.t1 MTR_7g079010 41.176 68 37 2 73 139 133 198 6.91e-11 61.2
MS.gene020902.t1 MTR_4g130900 46.667 75 40 0 186 260 344 418 1.61e-15 76.6
MS.gene020902.t1 MTR_1g015140 46.377 69 37 0 189 257 90 158 1.80e-15 72.8
MS.gene020902.t1 MTR_3g104750 44.928 69 38 0 189 257 92 160 4.02e-15 71.6
MS.gene020902.t1 MTR_8g007270 43.243 74 42 0 186 259 139 212 8.34e-15 72.4
MS.gene020902.t1 MTR_4g094588 46.667 75 40 0 187 261 164 238 1.18e-14 73.6
MS.gene020902.t1 MTR_1g086790 44.737 76 42 0 186 261 143 218 1.40e-14 73.2
MS.gene020902.t1 MTR_2g033820 43.421 76 43 0 186 261 188 263 1.44e-14 73.6
MS.gene020902.t1 MTR_8g096730 33.333 90 60 0 168 257 37 126 2.20e-14 69.3
MS.gene020902.t1 MTR_8g092010 41.667 72 42 0 189 260 96 167 2.26e-14 70.1
MS.gene020902.t1 MTR_5g043880 41.489 94 50 2 186 275 109 201 2.63e-14 71.6
MS.gene020902.t1 MTR_3g095040 40.541 74 44 0 187 260 104 177 1.33e-13 68.6
MS.gene020902.t1 MTR_5g091390 44.304 79 43 1 187 264 143 221 1.75e-13 69.7
MS.gene020902.t1 MTR_3g009470 47.458 59 31 0 83 141 280 338 1.81e-13 70.5
MS.gene020902.t1 MTR_3g095080 41.892 74 43 0 186 259 129 202 2.16e-13 68.2
MS.gene020902.t1 MTR_3g095080 41.892 74 43 0 186 259 153 226 2.81e-13 68.2
MS.gene020902.t1 MTR_7g109800 42.667 75 43 0 186 260 130 204 4.55e-13 68.6
MS.gene020902.t1 MTR_7g028415 42.647 68 39 0 190 257 82 149 4.95e-13 66.2
MS.gene020902.t1 MTR_7g028710 42.647 68 39 0 190 257 82 149 4.95e-13 66.2
MS.gene020902.t1 MTR_8g092140 44.000 75 41 1 186 259 144 218 1.45e-12 66.2
MS.gene020902.t1 MTR_2g105060 36.471 85 53 1 178 261 71 155 2.86e-12 65.5
MS.gene020902.t1 MTR_1g099600 38.298 94 52 2 195 282 179 272 3.31e-12 65.9
MS.gene020902.t1 MTR_4g007060 36.170 94 51 3 185 269 112 205 4.65e-12 65.5
MS.gene020902.t1 MTR_2g105060 36.471 85 53 1 178 261 146 230 5.15e-12 65.5
MS.gene020902.t1 MTR_4g007060 36.170 94 51 3 185 269 146 239 5.54e-12 65.5
MS.gene020902.t1 MTR_3g106060 43.056 72 38 2 189 257 124 195 9.50e-12 64.7
MS.gene020902.t1 MTR_7g110720 37.333 75 45 2 199 271 187 261 1.70e-11 64.3
MS.gene020902.t1 MTR_7g110720 37.333 75 45 2 199 271 214 288 1.81e-11 64.3
MS.gene020902.t1 MTR_3g053330 25.731 171 109 6 112 280 177 331 3.16e-11 63.5
MS.gene020902.t1 MTR_5g074200 44.000 75 42 0 71 145 208 282 3.39e-11 63.2
MS.gene020902.t1 MTR_5g074200 40.000 70 41 1 201 270 220 288 6.53e-11 62.4
MS.gene020902.t1 MTR_4g122530 44.262 61 34 0 200 260 207 267 3.66e-11 63.5
MS.gene020902.t1 MTR_1g013760 45.070 71 37 2 189 257 127 197 3.80e-11 62.8
MS.gene020902.t1 MTR_6g086555 37.500 80 49 1 182 260 187 266 5.36e-11 62.8
MS.gene020902.t1 MTR_4g094412 41.538 65 37 1 199 262 232 296 7.01e-11 62.8
MS.gene020902.t1 MTR_4g094412 41.538 65 37 1 199 262 241 305 7.08e-11 62.8
MS.gene020902.t1 MTR_4g049790 32.911 79 52 1 185 262 296 374 9.25e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020902.t1 AT2G04880 32.180 289 142 3 47 281 75 363 1.27e-44 157
MS.gene020902.t1 AT5G07100 35.610 205 120 5 65 263 5 203 7.99e-36 128
MS.gene020902.t1 AT5G07100 35.610 205 120 5 65 263 5 203 7.99e-36 128
MS.gene020902.t1 AT5G07100 35.610 205 120 5 65 263 5 203 7.99e-36 128
MS.gene020902.t1 AT5G07100 35.610 205 120 5 65 263 5 203 7.99e-36 128
MS.gene020902.t1 AT5G07100 35.610 205 120 5 65 263 98 296 4.33e-35 128
MS.gene020902.t1 AT3G01080 35.354 198 107 4 84 260 168 365 9.96e-34 127
MS.gene020902.t1 AT3G01080 35.354 198 107 4 84 260 206 403 1.18e-33 127
MS.gene020902.t1 AT2G38470 31.405 242 106 5 83 264 184 425 9.27e-31 120
MS.gene020902.t1 AT4G30935 30.804 224 108 5 83 260 168 390 1.17e-24 103
MS.gene020902.t1 AT2G04880 37.162 148 83 2 144 281 240 387 2.36e-24 102
MS.gene020902.t1 AT2G04880 39.604 101 61 0 47 147 75 175 2.46e-18 85.1
MS.gene020902.t1 AT2G04880 44.118 68 37 1 198 265 108 174 2.02e-12 67.4
MS.gene020902.t1 AT5G56270 47.436 78 41 0 186 263 472 549 1.13e-20 92.0
MS.gene020902.t1 AT5G56270 42.373 59 34 0 83 141 273 331 9.23e-11 62.8
MS.gene020902.t1 AT2G03340 41.111 90 53 0 185 274 399 488 1.19e-19 89.0
MS.gene020902.t1 AT2G03340 42.857 84 47 1 192 275 241 323 6.37e-15 75.1
MS.gene020902.t1 AT2G03340 49.153 59 30 0 84 142 251 309 1.10e-12 68.2
MS.gene020902.t1 AT1G13960 44.737 76 42 0 185 260 240 315 2.37e-19 87.0
MS.gene020902.t1 AT1G13960 44.706 85 44 2 188 270 63 146 3.55e-15 75.1
MS.gene020902.t1 AT1G13960 47.541 61 32 0 84 144 77 137 1.68e-12 67.4
MS.gene020902.t1 AT1G13960 44.737 76 42 0 185 260 393 468 2.93e-19 87.8
MS.gene020902.t1 AT1G13960 44.706 85 44 2 188 270 216 299 3.00e-15 75.9
MS.gene020902.t1 AT1G13960 47.541 61 32 0 84 144 230 290 9.59e-13 68.6
MS.gene020902.t1 AT2G30250 49.351 77 39 0 186 262 313 389 7.43e-19 85.9
MS.gene020902.t1 AT2G30250 42.188 64 37 0 84 147 167 230 8.55e-13 68.2
MS.gene020902.t1 AT4G26440 41.304 92 54 0 186 277 357 448 1.52e-18 85.9
MS.gene020902.t1 AT4G26440 46.552 58 31 0 84 141 179 236 8.06e-12 65.9
MS.gene020902.t1 AT4G26640 46.154 78 42 0 186 263 190 267 3.42e-18 84.0
MS.gene020902.t1 AT4G26640 41.558 77 44 1 67 142 18 94 2.64e-14 72.8
MS.gene020902.t1 AT4G26640 38.889 90 51 3 194 282 28 114 1.05e-11 65.1
MS.gene020902.t1 AT4G26640 46.154 78 42 0 186 263 190 267 3.42e-18 84.0
MS.gene020902.t1 AT4G26640 41.558 77 44 1 67 142 18 94 2.64e-14 72.8
MS.gene020902.t1 AT4G26640 38.889 90 51 3 194 282 28 114 1.05e-11 65.1
MS.gene020902.t1 AT4G26640 46.154 78 42 0 186 263 190 267 3.42e-18 84.0
MS.gene020902.t1 AT4G26640 41.558 77 44 1 67 142 18 94 2.64e-14 72.8
MS.gene020902.t1 AT4G26640 38.889 90 51 3 194 282 28 114 1.05e-11 65.1
MS.gene020902.t1 AT4G26640 46.154 78 42 0 186 263 190 267 3.42e-18 84.0
MS.gene020902.t1 AT4G26640 41.558 77 44 1 67 142 18 94 2.64e-14 72.8
MS.gene020902.t1 AT4G26640 38.889 90 51 3 194 282 28 114 1.05e-11 65.1
MS.gene020902.t1 AT4G26640 46.154 78 42 0 186 263 326 403 4.26e-18 84.3
MS.gene020902.t1 AT4G26640 41.558 77 44 1 67 142 154 230 1.37e-14 73.9
MS.gene020902.t1 AT4G26640 38.889 90 51 3 194 282 164 250 8.66e-12 65.5
MS.gene020902.t1 AT4G26640 46.154 78 42 0 186 263 366 443 4.51e-18 84.3
MS.gene020902.t1 AT4G26640 41.558 77 44 1 67 142 194 270 1.56e-14 73.9
MS.gene020902.t1 AT4G26640 38.889 90 51 3 194 282 204 290 9.51e-12 65.5
MS.gene020902.t1 AT4G26640 46.154 78 42 0 186 263 294 371 4.52e-18 84.3
MS.gene020902.t1 AT4G26640 41.558 77 44 1 67 142 122 198 1.91e-14 73.6
MS.gene020902.t1 AT4G26640 38.889 90 51 3 194 282 132 218 9.50e-12 65.5
MS.gene020902.t1 AT5G13080 44.118 68 38 0 190 257 56 123 9.17e-15 70.5
MS.gene020902.t1 AT2G44745 43.243 74 42 0 186 259 130 203 1.43e-14 71.6
MS.gene020902.t1 AT2G44745 43.662 71 39 1 81 150 143 213 2.95e-11 62.4
MS.gene020902.t1 AT5G64810 40.845 71 42 0 189 259 98 168 1.83e-14 70.9
MS.gene020902.t1 AT5G26170 43.478 69 39 0 189 257 101 169 3.39e-14 69.7
MS.gene020902.t1 AT4G18170 46.053 76 41 0 186 261 157 232 6.18e-14 71.2
MS.gene020902.t1 AT1G55600 44.444 72 40 0 189 260 295 366 7.36e-14 71.6
MS.gene020902.t1 AT1G69310 43.836 73 41 0 186 258 132 204 8.79e-14 70.5
MS.gene020902.t1 AT1G69310 43.836 73 41 0 186 258 132 204 8.79e-14 70.5
MS.gene020902.t1 AT1G69310 43.836 73 41 0 186 258 132 204 8.79e-14 70.5
MS.gene020902.t1 AT5G46350 43.529 85 48 0 187 271 169 253 9.86e-14 70.5
MS.gene020902.t1 AT3G01080 49.153 59 30 0 84 142 206 264 1.32e-13 70.1
MS.gene020902.t1 AT5G49520 45.000 80 43 1 186 264 206 285 1.90e-13 70.1
MS.gene020902.t1 AT4G39410 43.243 74 42 0 186 259 208 281 2.89e-13 68.9
MS.gene020902.t1 AT2G47260 42.667 75 43 0 186 260 159 233 3.04e-13 69.3
MS.gene020902.t1 AT5G41570 42.254 71 41 0 187 257 84 154 3.12e-13 67.0
MS.gene020902.t1 AT5G41570 45.000 60 32 1 81 139 96 155 9.12e-11 60.1
MS.gene020902.t1 AT2G21900 38.158 76 45 1 188 261 94 169 4.91e-13 67.0
MS.gene020902.t1 AT2G46130 39.437 71 43 0 187 257 16 86 1.55e-12 63.2
MS.gene020902.t1 AT3G62340 47.222 72 36 1 191 260 106 177 1.77e-12 66.6
MS.gene020902.t1 AT4G23550 41.333 75 43 1 190 263 129 203 2.15e-12 66.6
MS.gene020902.t1 AT1G64000 42.029 69 40 0 189 257 102 170 3.23e-12 64.7
MS.gene020902.t1 AT1G64000 45.000 60 32 1 81 139 112 171 3.70e-11 61.6
MS.gene020902.t1 AT2G37260 34.831 89 58 0 169 257 237 325 3.51e-12 66.2
MS.gene020902.t1 AT2G37260 46.552 58 31 0 84 141 86 143 4.02e-11 63.2
MS.gene020902.t1 AT2G37260 41.429 70 40 1 192 261 76 144 7.50e-11 62.4
MS.gene020902.t1 AT2G37260 34.831 89 58 0 169 257 235 323 3.64e-12 66.2
MS.gene020902.t1 AT2G37260 46.552 58 31 0 84 141 84 141 4.54e-11 62.8
MS.gene020902.t1 AT2G37260 41.429 70 40 1 192 261 74 142 7.79e-11 62.4
MS.gene020902.t1 AT1G29860 41.975 81 46 1 186 265 121 201 4.65e-12 65.5
MS.gene020902.t1 AT2G37260 39.706 68 41 0 190 257 338 405 5.97e-12 65.9
MS.gene020902.t1 AT2G37260 46.552 58 31 0 84 141 166 223 2.20e-11 64.3
MS.gene020902.t1 AT2G37260 41.429 70 40 1 192 261 156 224 9.34e-11 62.4
MS.gene020902.t1 AT3G01970 41.176 68 40 0 190 257 54 121 6.97e-12 62.4
MS.gene020902.t1 AT2G25000 36.364 88 52 2 185 268 87 174 9.04e-12 63.9
MS.gene020902.t1 AT2G25000 36.364 88 52 2 185 268 87 174 9.04e-12 63.9
MS.gene020902.t1 AT2G25000 36.364 88 52 2 185 268 109 196 1.19e-11 63.9
MS.gene020902.t1 AT2G25000 36.364 88 52 2 185 268 130 217 1.32e-11 63.9
MS.gene020902.t1 AT4G01720 42.254 71 40 1 191 260 229 299 2.05e-11 64.3
MS.gene020902.t1 AT1G68150 32.222 90 60 1 191 279 225 314 2.77e-11 63.5
MS.gene020902.t1 AT4G31800 32.979 94 59 2 191 280 101 194 3.56e-11 62.4
MS.gene020902.t1 AT4G31800 32.979 94 59 2 191 280 166 259 4.47e-11 62.8
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 5.95e-11 63.5
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 5.95e-11 63.5
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.21e-11 63.5
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.21e-11 63.5
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.53e-11 63.2
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.53e-11 63.2
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.53e-11 63.2
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.56e-11 63.2
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 383 459 6.60e-11 63.2
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.81e-11 63.2
MS.gene020902.t1 AT4G12020 39.744 78 46 1 183 260 450 526 6.95e-11 63.2
MS.gene020902.t1 AT1G80840 30.137 146 88 4 128 261 64 207 7.93e-11 62.0

Find 58 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAATGTGACCTCCCTTTC+AGG 0.167329 5.4:+76725847 None:intergenic
AATTCATGTGAAGGAGTATT+AGG 0.224685 5.4:+76725745 None:intergenic
AACTTCCACACCGGCCAAAT+AGG 0.232568 5.4:+76725426 None:intergenic
ACTCAAATAATGCGTATTAA+TGG 0.253581 5.4:-76724859 MS.gene020902:CDS
TAATGTAACAACTACCTATA+AGG 0.261776 5.4:-76724941 MS.gene020902:CDS
ATGATCATGTTGTCCCTTAT+AGG 0.293854 5.4:+76724927 None:intergenic
GGGACCACATCAACCCTATT+TGG 0.297637 5.4:-76725440 MS.gene020902:CDS
TATTGATTAGATTGTATTAG+AGG 0.320863 5.4:+76725721 None:intergenic
TGAATGTATGCATCTGTAAT+AGG 0.347318 5.4:+76725603 None:intergenic
TGTTCTTCCTGTTGTTGAAC+AGG 0.360275 5.4:-76725461 MS.gene020902:CDS
ACAATGCTTGATTGAGGATT+AGG 0.367320 5.4:+76725499 None:intergenic
GTTACATTAGTCACATTTAG+AGG 0.369899 5.4:+76724955 None:intergenic
ACTTCCACACCGGCCAAATA+GGG 0.411577 5.4:+76725427 None:intergenic
AAATTTCATTGGTCAAATGA+TGG 0.419177 5.4:-76725562 MS.gene020902:CDS
TCGCTCAATCATACGTGAAA+TGG 0.436400 5.4:-76725695 MS.gene020902:CDS
TACTTTCGATGCGCCTATCC+AGG 0.436737 5.4:-76725009 MS.gene020902:CDS
AATGTAACAACTACCTATAA+GGG 0.449073 5.4:-76724940 MS.gene020902:CDS
ATTCATGTGAAGGAGTATTA+GGG 0.450455 5.4:+76725746 None:intergenic
TTGTAAATGATGCGTATCGC+TGG 0.453288 5.4:-76725235 MS.gene020902:CDS
CGTGAAATGGTAAGAAAAGA+TGG 0.461858 5.4:-76725682 MS.gene020902:CDS
GCGTATCGCTGGCGCAAATA+CGG 0.465731 5.4:-76725224 MS.gene020902:CDS
ATTAGGATGATTATGCTGAT+TGG 0.483420 5.4:+76725516 None:intergenic
TTGGCCGGTGTGGAAGTTCA+AGG 0.489911 5.4:-76725421 MS.gene020902:intron
TGTGGTCCCTGTTCAACAAC+AGG 0.490907 5.4:+76725454 None:intergenic
TTTCACTGTGCATCCTGGAT+AGG 0.494274 5.4:+76724996 None:intergenic
ACAGGAAGAACATGATCAAC+CGG 0.497996 5.4:+76725472 None:intergenic
AATTTCATTGGTCAAATGAT+GGG 0.502281 5.4:-76725561 MS.gene020902:CDS
GTTCTTCCTGTTGTTGAACA+GGG 0.504635 5.4:-76725460 MS.gene020902:CDS
ATGCATACATTCAGACTGTC+GGG 0.523126 5.4:-76725593 MS.gene020902:CDS
CCACATCAACCCTATTTGGC+CGG 0.528483 5.4:-76725436 MS.gene020902:CDS
AGAGACTAATGATGTAGCTA+TGG 0.536005 5.4:-76725821 MS.gene020902:CDS
TTACATTAGTCACATTTAGA+GGG 0.537229 5.4:+76724956 None:intergenic
TAAGAAAAGATGGCTATAAG+TGG 0.538492 5.4:-76725672 MS.gene020902:CDS
AATACTGATGCTACTAGAGT+TGG 0.539414 5.4:-76725311 MS.gene020902:CDS
ACATCGAGTGAATCCTGAAA+GGG 0.553354 5.4:-76725860 MS.gene020902:CDS
TCATGAACCACCGACTGGTA+GGG 0.554218 5.4:-76724908 MS.gene020902:CDS
ATTAGAGGTAATTCATGTGA+AGG 0.561308 5.4:+76725736 None:intergenic
GGTGGTACAATGCTTGATTG+AGG 0.561784 5.4:+76725493 None:intergenic
ACTCGATGTTGTACTTCAGA+AGG 0.565037 5.4:+76725874 None:intergenic
GAAGTTTGAACAACAAGGTG+AGG 0.569592 5.4:+76725272 None:intergenic
TGTAGCTATGGCTTGTGACT+TGG 0.571450 5.4:-76725809 MS.gene020902:CDS
TCAATCAAGCATTGTACCAC+CGG 0.571503 5.4:-76725491 MS.gene020902:CDS
ATCATGAACCACCGACTGGT+AGG 0.573060 5.4:-76724909 MS.gene020902:CDS
GATGCATACATTCAGACTGT+CGG 0.573245 5.4:-76725594 MS.gene020902:CDS
CATGAACCACCGACTGGTAG+GGG 0.577060 5.4:-76724907 MS.gene020902:CDS
CTCTGCGAGTGTTGCATGAG+TGG 0.578417 5.4:+76725769 None:intergenic
CAGTTGAAGTTTGAACAACA+AGG 0.606492 5.4:+76725267 None:intergenic
AACATCGAGTGAATCCTGAA+AGG 0.613815 5.4:-76725861 MS.gene020902:CDS
TCAACCCTATTTGGCCGGTG+TGG 0.617265 5.4:-76725431 MS.gene020902:CDS
CCGGCCAAATAGGGTTGATG+TGG 0.620302 5.4:+76725436 None:intergenic
TACATTAGTCACATTTAGAG+GGG 0.623510 5.4:+76724957 None:intergenic
CATGATCATGAACCACCGAC+TGG 0.628103 5.4:-76724913 MS.gene020902:CDS
TATTTGTAGCAGAGTCATGA+CGG 0.637103 5.4:+76724881 None:intergenic
TCGAGTGAATCCTGAAAGGG+AGG 0.657624 5.4:-76725857 MS.gene020902:CDS
ATCACCTTGAACTTCCACAC+CGG 0.664656 5.4:+76725417 None:intergenic
CGGACACCCCTACCAGTCGG+TGG 0.689482 5.4:+76724901 None:intergenic
TGACGGACACCCCTACCAGT+CGG 0.690312 5.4:+76724898 None:intergenic
GGAAGAACATGATCAACCGG+TGG 0.725841 5.4:+76725475 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATGTACAATATATTAAG+AGG + chr5.4:76725415-76725434 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG - chr5.4:76725572-76725591 MS.gene020902:CDS 20.0%
!!! TATTGATTAGATTGTATTAG+AGG + chr5.4:76725044-76725063 None:intergenic 20.0%
! AAATTTCATTGGTCAAATGA+TGG - chr5.4:76725200-76725219 MS.gene020902:CDS 25.0%
! AATGTAACAACTACCTATAA+GGG - chr5.4:76725822-76725841 MS.gene020902:CDS 25.0%
! AATTTCATTGGTCAAATGAT+GGG - chr5.4:76725201-76725220 MS.gene020902:CDS 25.0%
! ACTCAAATAATGCGTATTAA+TGG - chr5.4:76725903-76725922 MS.gene020902:CDS 25.0%
! TAATGTAACAACTACCTATA+AGG - chr5.4:76725821-76725840 MS.gene020902:CDS 25.0%
! TTACATTAGTCACATTTAGA+GGG + chr5.4:76725809-76725828 None:intergenic 25.0%
!! GAAAGTAATTTCTAGAAGAT+TGG + chr5.4:76725740-76725759 None:intergenic 25.0%
ATGAGTTCAAAACAAAAGCT+AGG + chr5.4:76725603-76725622 None:intergenic 30.0%
ATTAGAGGTAATTCATGTGA+AGG + chr5.4:76725029-76725048 None:intergenic 30.0%
ATTAGGATGATTATGCTGAT+TGG + chr5.4:76725249-76725268 None:intergenic 30.0%
GGCTATAAGTGGAAAAAATA+TGG - chr5.4:76725101-76725120 MS.gene020902:intron 30.0%
GGGCAAAAAAGAAATTTCAT+TGG - chr5.4:76725189-76725208 MS.gene020902:intron 30.0%
GTTACATTAGTCACATTTAG+AGG + chr5.4:76725810-76725829 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG - chr5.4:76725090-76725109 MS.gene020902:intron 30.0%
TACATTAGTCACATTTAGAG+GGG + chr5.4:76725808-76725827 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG - chr5.4:76725556-76725575 MS.gene020902:CDS 30.0%
TGAATGTATGCATCTGTAAT+AGG + chr5.4:76725162-76725181 None:intergenic 30.0%
!! AATTCATGTGAAGGAGTATT+AGG + chr5.4:76725020-76725039 None:intergenic 30.0%
!! ATTCATGTGAAGGAGTATTA+GGG + chr5.4:76725019-76725038 None:intergenic 30.0%
AATACTGATGCTACTAGAGT+TGG - chr5.4:76725451-76725470 MS.gene020902:CDS 35.0%
ACAATGCTTGATTGAGGATT+AGG + chr5.4:76725266-76725285 None:intergenic 35.0%
AGAGACTAATGATGTAGCTA+TGG - chr5.4:76724941-76724960 MS.gene020902:CDS 35.0%
ATGATCATGTTGTCCCTTAT+AGG + chr5.4:76725838-76725857 None:intergenic 35.0%
CAGTTGAAGTTTGAACAACA+AGG + chr5.4:76725498-76725517 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG - chr5.4:76725080-76725099 MS.gene020902:intron 35.0%
TATTTGTAGCAGAGTCATGA+CGG + chr5.4:76725884-76725903 None:intergenic 35.0%
AACATCGAGTGAATCCTGAA+AGG - chr5.4:76724901-76724920 MS.gene020902:CDS 40.0%
ACAGGAAGAACATGATCAAC+CGG + chr5.4:76725293-76725312 None:intergenic 40.0%
ACATCGAGTGAATCCTGAAA+GGG - chr5.4:76724902-76724921 MS.gene020902:CDS 40.0%
ACTCGATGTTGTACTTCAGA+AGG + chr5.4:76724891-76724910 None:intergenic 40.0%
ATGCATACATTCAGACTGTC+GGG - chr5.4:76725169-76725188 MS.gene020902:intron 40.0%
GAAGTTTGAACAACAAGGTG+AGG + chr5.4:76725493-76725512 None:intergenic 40.0%
GATGCATACATTCAGACTGT+CGG - chr5.4:76725168-76725187 MS.gene020902:intron 40.0%
GCGCAAATACGGAAGAAAAA+TGG - chr5.4:76725549-76725568 MS.gene020902:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG - chr5.4:76725302-76725321 MS.gene020902:CDS 40.0%
TCGCTCAATCATACGTGAAA+TGG - chr5.4:76725067-76725086 MS.gene020902:intron 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG - chr5.4:76725301-76725320 MS.gene020902:CDS 40.0%
TTGTAAATGATGCGTATCGC+TGG - chr5.4:76725527-76725546 MS.gene020902:CDS 40.0%
! TCAATCAAGCATTGTACCAC+CGG - chr5.4:76725271-76725290 MS.gene020902:CDS 40.0%
!!! TACTTTTTCACTGTGCATCC+TGG + chr5.4:76725774-76725793 None:intergenic 40.0%
ATCACCTTGAACTTCCACAC+CGG + chr5.4:76725348-76725367 None:intergenic 45.0%
GGTGGTACAATGCTTGATTG+AGG + chr5.4:76725272-76725291 None:intergenic 45.0%
TTTCACTGTGCATCCTGGAT+AGG + chr5.4:76725769-76725788 None:intergenic 45.0%
! TGTAGCTATGGCTTGTGACT+TGG - chr5.4:76724953-76724972 MS.gene020902:CDS 45.0%
AACTTCCACACCGGCCAAAT+AGG + chr5.4:76725339-76725358 None:intergenic 50.0%
ACTTCCACACCGGCCAAATA+GGG + chr5.4:76725338-76725357 None:intergenic 50.0%
CATGATCATGAACCACCGAC+TGG - chr5.4:76725849-76725868 MS.gene020902:CDS 50.0%
CCACATCAACCCTATTTGGC+CGG - chr5.4:76725326-76725345 MS.gene020902:intron 50.0%
GACAATGTGACCTCCCTTTC+AGG + chr5.4:76724918-76724937 None:intergenic 50.0%
GGAAGAACATGATCAACCGG+TGG + chr5.4:76725290-76725309 None:intergenic 50.0%
GGGACCACATCAACCCTATT+TGG - chr5.4:76725322-76725341 MS.gene020902:intron 50.0%
TACTTTCGATGCGCCTATCC+AGG - chr5.4:76725753-76725772 MS.gene020902:CDS 50.0%
TCGAGTGAATCCTGAAAGGG+AGG - chr5.4:76724905-76724924 MS.gene020902:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG + chr5.4:76725311-76725330 None:intergenic 50.0%
! ATCATGAACCACCGACTGGT+AGG - chr5.4:76725853-76725872 MS.gene020902:CDS 50.0%
! TCATGAACCACCGACTGGTA+GGG - chr5.4:76725854-76725873 MS.gene020902:CDS 50.0%
GCGTATCGCTGGCGCAAATA+CGG - chr5.4:76725538-76725557 MS.gene020902:CDS 55.0%
TCAACCCTATTTGGCCGGTG+TGG - chr5.4:76725331-76725350 MS.gene020902:intron 55.0%
TTGGCCGGTGTGGAAGTTCA+AGG - chr5.4:76725341-76725360 MS.gene020902:intron 55.0%
! CATGAACCACCGACTGGTAG+GGG - chr5.4:76725855-76725874 MS.gene020902:CDS 55.0%
! CTCTGCGAGTGTTGCATGAG+TGG + chr5.4:76724996-76725015 None:intergenic 55.0%
!! CCGGCCAAATAGGGTTGATG+TGG + chr5.4:76725329-76725348 None:intergenic 55.0%
! TGACGGACACCCCTACCAGT+CGG + chr5.4:76725867-76725886 None:intergenic 60.0%
CGGACACCCCTACCAGTCGG+TGG + chr5.4:76725864-76725883 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 76724849 76725935 76724849 ID=MS.gene020902
chr5.4 mRNA 76724849 76725935 76724849 ID=MS.gene020902.t1;Parent=MS.gene020902
chr5.4 exon 76725422 76725935 76725422 ID=MS.gene020902.t1.exon1;Parent=MS.gene020902.t1
chr5.4 CDS 76725422 76725935 76725422 ID=cds.MS.gene020902.t1;Parent=MS.gene020902.t1
chr5.4 exon 76725191 76725338 76725191 ID=MS.gene020902.t1.exon2;Parent=MS.gene020902.t1
chr5.4 CDS 76725191 76725338 76725191 ID=cds.MS.gene020902.t1;Parent=MS.gene020902.t1
chr5.4 exon 76724849 76725035 76724849 ID=MS.gene020902.t1.exon3;Parent=MS.gene020902.t1
chr5.4 CDS 76724849 76725035 76724849 ID=cds.MS.gene020902.t1;Parent=MS.gene020902.t1
Gene Sequence

>MS.gene020902

ATGGTTTTGTCACAACAAAATGTTGATCACAATGATTCTCCTTCTGAAGTACAACATCGAGTGAATCCTGAAAGGGAGGTCACATTGTCACAAGAGACTAATGATGTAGCTATGGCTTGTGACTTGGTATTATCACATTCTTCACCACTCATGCAACACTCGCAGAGCCCTAATACTCCTTCACATGAATTACCTCTAATACAATCTAATCAATACACTCGCTCAATCATACGTGAAATGGTAAGAAAAGATGGCTATAAGTGGAAAAAATATGGTGAGAAAAACATTAAGAAGAATGAACATAAAAGAGCCTATTACAGATGCATACATTCAGACTGTCGGGCAAAAAAGAAATTTCATTGGTCAAATGATGGGACTGTCGAATATTTTAGTTACACCAATCAGCATAATCATCCTAATCCTCAATCAAGCATTGTACCACCGGTTGATCATGTTCTTCCTGTTGTTGAACAGGGACCACATCAACCCTATTTGGCCGGTGTGGAAGTTCAAGGTGATAAGTACTCTTTTTTAGTATTTTAGTTTCAATATTACATGAAAAACCTCTTAATATATTGTACATTGTTGTGCATGCAGACAACAATACTGATGCTACTAGAGTTGGCGTGTTAACTAGTGAACCTCACCTTGTTGTTCAAACTTCAACTGCAAATGAAGTTGTAAATGATGCGTATCGCTGGCGCAAATACGGAAGAAAAATGGTTAATGGAAAAACAATTCAAAGGTACATCCTAGCTTTTGTTTTGAACTCATACATTGTAGATCATTTCAATATGCATTTTTTTTATTGCTTTTTCTCTGTGCTTTAAGCTTTTGCAAATACACAAATTCAAGAAATGATTTTATCTAATTGATTGCATTTTCTCTCCAATCTTCTAGAAATTACTTTCGATGCGCCTATCCAGGATGCACAGTGAAAAAGTATGTCGAAAAGTCCCCTCTAAATGTGACTAATGTAACAACTACCTATAAGGGACAACATGATCATGAACCACCGACTGGTAGGGGTGTCCGTCATGACTCTGCTACAAATACTCAAATAATGCGTATTAATGGCGAGCCATAA
Protein sequence

>MS.gene020902.t1

MVLSQQNVDHNDSPSEVQHRVNPEREVTLSQETNDVAMACDLVLSHSSPLMQHSQSPNTPSHELPLIQSNQYTRSIIREMVRKDGYKWKKYGEKNIKKNEHKRAYYRCIHSDCRAKKKFHWSNDGTVEYFSYTNQHNHPNPQSSIVPPVDHVLPVVEQGPHQPYLAGVEVQDNNTDATRVGVLTSEPHLVVQTSTANEVVNDAYRWRKYGRKMVNGKTIQRNYFRCAYPGCTVKKYVEKSPLNVTNVTTTYKGQHDHEPPTGRGVRHDSATNTQIMRINGEP