Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020971.t1 | XP_003618052.1 | 90.4 | 270 | 24 | 1 | 1 | 268 | 1 | 270 | 1.20E-113 | 419.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020971.t1 | G7K0M6 | 90.4 | 270 | 24 | 1 | 1 | 268 | 1 | 270 | 8.6e-114 | 419.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene020971.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020971.t1 | MTR_5g098470 | 90.741 | 270 | 23 | 1 | 1 | 268 | 1 | 270 | 1.12e-179 | 495 |
| MS.gene020971.t1 | MTR_1g046810 | 31.016 | 187 | 84 | 6 | 53 | 202 | 32 | 210 | 8.43e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020971.t1 | AT5G56200 | 44.048 | 84 | 41 | 1 | 60 | 137 | 77 | 160 | 1.11e-12 | 67.8 |
| MS.gene020971.t1 | AT2G26940 | 33.981 | 103 | 43 | 1 | 62 | 139 | 49 | 151 | 5.62e-12 | 65.1 |
Find 49 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATATTATTATCCTTATT+AGG | 0.131951 | 5.4:+77971144 | None:intergenic |
| ATACCCCTCCATTCCCTTTC+AGG | 0.172402 | 5.4:+77971171 | None:intergenic |
| GTGACCATGTAAAGCCTTAT+TGG | 0.177322 | 5.4:+77971206 | None:intergenic |
| ATCAAAAGATAATGTCTTAT+TGG | 0.200998 | 5.4:-77970779 | MS.gene020971:CDS |
| ATCATCAATAACCAATTCTT+TGG | 0.220906 | 5.4:+77971074 | None:intergenic |
| CAATATTATTATCCTTATTA+GGG | 0.247792 | 5.4:+77971145 | None:intergenic |
| CATCAAACTTATTCTTCTTT+TGG | 0.256389 | 5.4:+77971635 | None:intergenic |
| AATAACCAATTCTTTGGAAT+TGG | 0.272420 | 5.4:+77971080 | None:intergenic |
| TTTAGGTGTGGAAAAGGATT+AGG | 0.327731 | 5.4:-77971348 | MS.gene020971:CDS |
| TGCATCCAATTCCAAAGAAT+TGG | 0.334606 | 5.4:-77971085 | MS.gene020971:CDS |
| CTTTCCCTAGAGCTTGACTA+TGG | 0.341322 | 5.4:+77971310 | None:intergenic |
| TCATCTTGATGATGACCCTT+TGG | 0.345076 | 5.4:+77971420 | None:intergenic |
| TGACAACTTCAATGAGGATT+TGG | 0.347180 | 5.4:-77970935 | MS.gene020971:CDS |
| TTGATGATGAATCAAAATCT+TGG | 0.371768 | 5.4:-77971059 | MS.gene020971:CDS |
| GAGGGGTATGAACCCTAATA+AGG | 0.375613 | 5.4:-77971157 | MS.gene020971:CDS |
| TACCCCTCCATTCCCTTTCA+GGG | 0.375926 | 5.4:+77971172 | None:intergenic |
| GTTGTCATGTAAATGTTTAT+CGG | 0.376472 | 5.4:+77970951 | None:intergenic |
| CCTAGTACTGGTGGTCTTGT+TGG | 0.388190 | 5.4:-77970883 | MS.gene020971:CDS |
| TGATGATGAATCAAAATCTT+GGG | 0.427675 | 5.4:-77971058 | MS.gene020971:CDS |
| CATGTAAAGCCTTATTGGAT+TGG | 0.438633 | 5.4:+77971211 | None:intergenic |
| TTTGGATGATGAGGATAAGA+AGG | 0.451348 | 5.4:-77970917 | MS.gene020971:CDS |
| CAATCCAATAAGGCTTTACA+TGG | 0.457233 | 5.4:-77971210 | MS.gene020971:CDS |
| GCTAATTGCAATTCTAATGA+TGG | 0.457837 | 5.4:-77971267 | MS.gene020971:CDS |
| CAAAAGAAGAATAAGTTTGA+TGG | 0.471977 | 5.4:-77971634 | MS.gene020971:CDS |
| AGAATCCATAGTCAAGCTCT+AGG | 0.477759 | 5.4:-77971315 | MS.gene020971:CDS |
| TTGTTTAAAACCAGTAAAAG+AGG | 0.486875 | 5.4:-77971027 | MS.gene020971:CDS |
| CCAACAAGACCACCAGTACT+AGG | 0.502633 | 5.4:+77970883 | None:intergenic |
| CATGAGATCTCACCCTGAAA+GGG | 0.502988 | 5.4:-77971184 | MS.gene020971:CDS |
| GAGAATGTTGTACCTAGTAC+TGG | 0.518957 | 5.4:-77970895 | MS.gene020971:CDS |
| ACATGAGATCTCACCCTGAA+AGG | 0.521597 | 5.4:-77971185 | MS.gene020971:CDS |
| AAGGGTCATCATCAAGATGA+AGG | 0.523929 | 5.4:-77971417 | MS.gene020971:CDS |
| TGATGGTGGTAGGATACATC+AGG | 0.528563 | 5.4:-77971617 | MS.gene020971:intron |
| TAGTCAAGCTCTAGGGAAAG+AGG | 0.530133 | 5.4:-77971307 | MS.gene020971:CDS |
| AGAATGACAATAACAAAAGA+AGG | 0.532053 | 5.4:-77970837 | MS.gene020971:CDS |
| AGGTGTGGAAAAGGATTAGG+TGG | 0.533754 | 5.4:-77971345 | MS.gene020971:CDS |
| CAATGAGGATTTGGATGATG+AGG | 0.536568 | 5.4:-77970926 | MS.gene020971:CDS |
| AGAATAAGTTTGATGGTGGT+AGG | 0.553715 | 5.4:-77971627 | MS.gene020971:CDS |
| GATCTCACCCTGAAAGGGAA+TGG | 0.563550 | 5.4:-77971179 | MS.gene020971:CDS |
| GAATCCATAGTCAAGCTCTA+GGG | 0.567403 | 5.4:-77971314 | MS.gene020971:CDS |
| TCACCCTGAAAGGGAATGGA+GGG | 0.580204 | 5.4:-77971175 | MS.gene020971:CDS |
| CTCACCCTGAAAGGGAATGG+AGG | 0.583418 | 5.4:-77971176 | MS.gene020971:CDS |
| TAACAAAAGAAGGAAGAAGC+TGG | 0.586820 | 5.4:-77970827 | MS.gene020971:CDS |
| TTTACATGACAACTTCAATG+AGG | 0.602810 | 5.4:-77970941 | MS.gene020971:CDS |
| AGTCAAGCTCTAGGGAAAGA+GGG | 0.609576 | 5.4:-77971306 | MS.gene020971:CDS |
| AAGAAGAATAAGTTTGATGG+TGG | 0.622051 | 5.4:-77971631 | MS.gene020971:CDS |
| AGTAAAAGAGGTCGTTCGAG+TGG | 0.639877 | 5.4:-77971015 | MS.gene020971:CDS |
| CACCCTGAAAGGGAATGGAG+GGG | 0.655548 | 5.4:-77971174 | MS.gene020971:CDS |
| AGAGGGTGAGTCGAAAGCAA+AGG | 0.661254 | 5.4:-77971289 | MS.gene020971:CDS |
| AATGTTGTACCTAGTACTGG+TGG | 0.674829 | 5.4:-77970892 | MS.gene020971:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGTAACAAAATTTTT+AGG | - | chr5.4:77971058-77971077 | MS.gene020971:CDS | 10.0% |
| !! | CAATATTATTATCCTTATTA+GGG | + | chr5.4:77971281-77971300 | None:intergenic | 15.0% |
| !! | TCAATATTATTATCCTTATT+AGG | + | chr5.4:77971282-77971301 | None:intergenic | 15.0% |
| !!! | TTTCTTTTAATAGAATTCTA+GGG | - | chr5.4:77970906-77970925 | MS.gene020971:CDS | 15.0% |
| !!! | TTTTCTTTTAATAGAATTCT+AGG | - | chr5.4:77970905-77970924 | MS.gene020971:CDS | 15.0% |
| !! | AAAGTTTATGAAAAACCAAA+GGG | - | chr5.4:77970988-77971007 | MS.gene020971:CDS | 20.0% |
| !! | ATCAAAAGATAATGTCTTAT+TGG | - | chr5.4:77971644-77971663 | MS.gene020971:CDS | 20.0% |
| !! | TAAAGTTTATGAAAAACCAA+AGG | - | chr5.4:77970987-77971006 | MS.gene020971:CDS | 20.0% |
| ! | AAAAAACTTCCAATCCAATA+AGG | - | chr5.4:77971203-77971222 | MS.gene020971:CDS | 25.0% |
| ! | AGAATGACAATAACAAAAGA+AGG | - | chr5.4:77971586-77971605 | MS.gene020971:intron | 25.0% |
| ! | ATCATCAATAACCAATTCTT+TGG | + | chr5.4:77971352-77971371 | None:intergenic | 25.0% |
| ! | CAAAAGAAGAATAAGTTTGA+TGG | - | chr5.4:77970789-77970808 | MS.gene020971:CDS | 25.0% |
| ! | GTTGTCATGTAAATGTTTAT+CGG | + | chr5.4:77971475-77971494 | None:intergenic | 25.0% |
| !! | CATCAAACTTATTCTTCTTT+TGG | + | chr5.4:77970791-77970810 | None:intergenic | 25.0% |
| !! | TAAACTTTTCTTCTTCTCTA+TGG | + | chr5.4:77970947-77970966 | None:intergenic | 25.0% |
| !! | TGATGATGAATCAAAATCTT+GGG | - | chr5.4:77971365-77971384 | MS.gene020971:CDS | 25.0% |
| !! | TTATCTTTTGATGTTGAACA+TGG | + | chr5.4:77971636-77971655 | None:intergenic | 25.0% |
| !! | TTATTCTTCTTTTGGTCATT+GGG | + | chr5.4:77970783-77970802 | None:intergenic | 25.0% |
| !! | TTGTTTAAAACCAGTAAAAG+AGG | - | chr5.4:77971396-77971415 | MS.gene020971:CDS | 25.0% |
| !!! | AAAATTTTTAGGTGTGGAAA+AGG | - | chr5.4:77971069-77971088 | MS.gene020971:CDS | 25.0% |
| !!! | AATAACCAATTCTTTGGAAT+TGG | + | chr5.4:77971346-77971365 | None:intergenic | 25.0% |
| !!! | TGTAACAAAATTTTTAGGTG+TGG | - | chr5.4:77971063-77971082 | MS.gene020971:CDS | 25.0% |
| !!! | TTGATGATGAATCAAAATCT+TGG | - | chr5.4:77971364-77971383 | MS.gene020971:CDS | 25.0% |
| AAGAAGAATAAGTTTGATGG+TGG | - | chr5.4:77970792-77970811 | MS.gene020971:CDS | 30.0% | |
| GCTAATTGCAATTCTAATGA+TGG | - | chr5.4:77971156-77971175 | MS.gene020971:CDS | 30.0% | |
| TTTACATGACAACTTCAATG+AGG | - | chr5.4:77971482-77971501 | MS.gene020971:CDS | 30.0% | |
| ! | CTTATTCTTCTTTTGGTCAT+TGG | + | chr5.4:77970784-77970803 | None:intergenic | 30.0% |
| CAATCCAATAAGGCTTTACA+TGG | - | chr5.4:77971213-77971232 | MS.gene020971:CDS | 35.0% | |
| TAACAAAAGAAGGAAGAAGC+TGG | - | chr5.4:77971596-77971615 | MS.gene020971:intron | 35.0% | |
| TGACAACTTCAATGAGGATT+TGG | - | chr5.4:77971488-77971507 | MS.gene020971:CDS | 35.0% | |
| TGCATCCAATTCCAAAGAAT+TGG | - | chr5.4:77971338-77971357 | MS.gene020971:CDS | 35.0% | |
| TTTAGGTGTGGAAAAGGATT+AGG | - | chr5.4:77971075-77971094 | MS.gene020971:CDS | 35.0% | |
| TTTGGATGATGAGGATAAGA+AGG | - | chr5.4:77971506-77971525 | MS.gene020971:CDS | 35.0% | |
| ! | CTTCTTCTCTATGGTTTTCA+TGG | + | chr5.4:77970938-77970957 | None:intergenic | 35.0% |
| !! | AGAATAAGTTTGATGGTGGT+AGG | - | chr5.4:77970796-77970815 | MS.gene020971:CDS | 35.0% |
| !! | CATGTAAAGCCTTATTGGAT+TGG | + | chr5.4:77971215-77971234 | None:intergenic | 35.0% |
| AAGGGTCATCATCAAGATGA+AGG | - | chr5.4:77971006-77971025 | MS.gene020971:CDS | 40.0% | |
| AGAATCCATAGTCAAGCTCT+AGG | - | chr5.4:77971108-77971127 | MS.gene020971:CDS | 40.0% | |
| CAATGAGGATTTGGATGATG+AGG | - | chr5.4:77971497-77971516 | MS.gene020971:CDS | 40.0% | |
| GAATCCATAGTCAAGCTCTA+GGG | - | chr5.4:77971109-77971128 | MS.gene020971:CDS | 40.0% | |
| GAGAATGTTGTACCTAGTAC+TGG | - | chr5.4:77971528-77971547 | MS.gene020971:intron | 40.0% | |
| ! | AATGTTGTACCTAGTACTGG+TGG | - | chr5.4:77971531-77971550 | MS.gene020971:intron | 40.0% |
| ! | GTGACCATGTAAAGCCTTAT+TGG | + | chr5.4:77971220-77971239 | None:intergenic | 40.0% |
| !! | TCATCTTGATGATGACCCTT+TGG | + | chr5.4:77971006-77971025 | None:intergenic | 40.0% |
| ACATGAGATCTCACCCTGAA+AGG | - | chr5.4:77971238-77971257 | MS.gene020971:CDS | 45.0% | |
| AGGTGTGGAAAAGGATTAGG+TGG | - | chr5.4:77971078-77971097 | MS.gene020971:CDS | 45.0% | |
| AGTAAAAGAGGTCGTTCGAG+TGG | - | chr5.4:77971408-77971427 | MS.gene020971:CDS | 45.0% | |
| CATGAGATCTCACCCTGAAA+GGG | - | chr5.4:77971239-77971258 | MS.gene020971:CDS | 45.0% | |
| CTTTCCCTAGAGCTTGACTA+TGG | + | chr5.4:77971116-77971135 | None:intergenic | 45.0% | |
| ! | ACTCGAACGACCTCTTTTAC+TGG | + | chr5.4:77971409-77971428 | None:intergenic | 45.0% |
| ! | GAGGGGTATGAACCCTAATA+AGG | - | chr5.4:77971266-77971285 | MS.gene020971:CDS | 45.0% |
| !! | AGTCAAGCTCTAGGGAAAGA+GGG | - | chr5.4:77971117-77971136 | MS.gene020971:CDS | 45.0% |
| !! | TAGTCAAGCTCTAGGGAAAG+AGG | - | chr5.4:77971116-77971135 | MS.gene020971:CDS | 45.0% |
| !! | TGATGGTGGTAGGATACATC+AGG | - | chr5.4:77970806-77970825 | MS.gene020971:CDS | 45.0% |
| !! | ATAAAGAATATTAAATATAA+AGG | + | chr5.4:77970847-77970866 | None:intergenic | 5.0% |
| AGAGGGTGAGTCGAAAGCAA+AGG | - | chr5.4:77971134-77971153 | MS.gene020971:CDS | 50.0% | |
| ATACCCCTCCATTCCCTTTC+AGG | + | chr5.4:77971255-77971274 | None:intergenic | 50.0% | |
| CCAACAAGACCACCAGTACT+AGG | + | chr5.4:77971543-77971562 | None:intergenic | 50.0% | |
| GATCTCACCCTGAAAGGGAA+TGG | - | chr5.4:77971244-77971263 | MS.gene020971:CDS | 50.0% | |
| TACCCCTCCATTCCCTTTCA+GGG | + | chr5.4:77971254-77971273 | None:intergenic | 50.0% | |
| TCACCCTGAAAGGGAATGGA+GGG | - | chr5.4:77971248-77971267 | MS.gene020971:CDS | 50.0% | |
| !! | CCTAGTACTGGTGGTCTTGT+TGG | - | chr5.4:77971540-77971559 | MS.gene020971:intron | 50.0% |
| CACCCTGAAAGGGAATGGAG+GGG | - | chr5.4:77971249-77971268 | MS.gene020971:CDS | 55.0% | |
| CTCACCCTGAAAGGGAATGG+AGG | - | chr5.4:77971247-77971266 | MS.gene020971:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 77970774 | 77971671 | 77970774 | ID=MS.gene020971 |
| chr5.4 | mRNA | 77970774 | 77971671 | 77970774 | ID=MS.gene020971.t1;Parent=MS.gene020971 |
| chr5.4 | exon | 77971618 | 77971671 | 77971618 | ID=MS.gene020971.t1.exon1;Parent=MS.gene020971.t1 |
| chr5.4 | CDS | 77971618 | 77971671 | 77971618 | ID=cds.MS.gene020971.t1;Parent=MS.gene020971.t1 |
| chr5.4 | exon | 77970774 | 77971526 | 77970774 | ID=MS.gene020971.t1.exon2;Parent=MS.gene020971.t1 |
| chr5.4 | CDS | 77970774 | 77971526 | 77970774 | ID=cds.MS.gene020971.t1;Parent=MS.gene020971.t1 |
| Gene Sequence |
| Protein sequence |
