Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021069.t1 | RHN49639.1 | 69.2 | 159 | 27 | 3 | 54 | 191 | 10 | 167 | 7.90E-51 | 210.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021069.t1 | A0A396H8N5 | 69.2 | 159 | 27 | 3 | 54 | 191 | 10 | 167 | 5.7e-51 | 210.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene021069.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021069.t1 | MTR_7g117590 | 69.872 | 156 | 26 | 3 | 56 | 191 | 18 | 172 | 1.13e-68 | 207 |
| MS.gene021069.t1 | MTR_1g054470 | 48.649 | 185 | 36 | 5 | 1 | 180 | 3 | 133 | 3.62e-48 | 154 |
| MS.gene021069.t1 | MTR_2g012700 | 37.273 | 110 | 68 | 1 | 83 | 191 | 11 | 120 | 9.60e-28 | 101 |
| MS.gene021069.t1 | MTR_2g062330 | 39.316 | 117 | 66 | 3 | 76 | 191 | 27 | 139 | 1.90e-26 | 99.0 |
| MS.gene021069.t1 | MTR_5g071400 | 70.213 | 47 | 8 | 2 | 56 | 97 | 14 | 59 | 2.16e-14 | 66.2 |
| MS.gene021069.t1 | MTR_0010s0310 | 33.333 | 90 | 57 | 1 | 95 | 181 | 151 | 240 | 1.42e-13 | 67.4 |
| MS.gene021069.t1 | MTR_6g477950 | 36.275 | 102 | 61 | 3 | 90 | 190 | 46 | 144 | 3.55e-12 | 63.9 |
| MS.gene021069.t1 | MTR_6g087720 | 29.915 | 117 | 81 | 1 | 63 | 178 | 182 | 298 | 8.65e-12 | 62.8 |
| MS.gene021069.t1 | MTR_5g078270 | 31.461 | 89 | 58 | 1 | 96 | 181 | 210 | 298 | 3.46e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATCAACAATTCGAATTT+TGG | 0.069208 | 4.3:-40498844 | None:intergenic |
| CCATCGACTTCTTCTTCATT+TGG | 0.249648 | 4.3:-40498569 | None:intergenic |
| GTGGATGGTTTGAGTTTGTT+AGG | 0.273672 | 4.3:+40498934 | MS.gene021069:CDS |
| TGAAGATCGGGTCATTTGAT+TGG | 0.360270 | 4.3:+40498249 | MS.gene021069:CDS |
| CGAATTGTTGATGATGAAAC+TGG | 0.373851 | 4.3:+40498852 | MS.gene021069:CDS |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 4.3:+40498885 | MS.gene021069:CDS |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 4.3:+40498892 | MS.gene021069:CDS |
| GGCTGCTGGACGAAGAGTTC+TGG | 0.395341 | 4.3:+40498667 | MS.gene021069:CDS |
| TTTGAAATTGTGCAAATACT+TGG | 0.402550 | 4.3:-40498592 | None:intergenic |
| ATTAGTTCTGCTACGGCTGC+TGG | 0.408831 | 4.3:+40498653 | MS.gene021069:CDS |
| GAAGATCGGGTCATTTGATT+GGG | 0.427759 | 4.3:+40498250 | MS.gene021069:CDS |
| CGAAGAAGAAGATGAAGATC+GGG | 0.478220 | 4.3:+40498237 | MS.gene021069:CDS |
| TGGATGGTTTGAGTTTGTTA+GGG | 0.484898 | 4.3:+40498935 | MS.gene021069:CDS |
| ATGAAATGTGCGGCGGATGC+AGG | 0.509157 | 4.3:+40498186 | MS.gene021069:CDS |
| CTGCAGCGATGTAATTGATC+CGG | 0.509939 | 4.3:+40498514 | MS.gene021069:intron |
| CTTCTTCGTCCATCTCAAAA+CGG | 0.536060 | 4.3:-40498222 | None:intergenic |
| ACGAAGAAGAAGATGAAGAT+CGG | 0.540825 | 4.3:+40498236 | MS.gene021069:CDS |
| CGATGTAATTGATCCGGAGT+TGG | 0.544205 | 4.3:+40498520 | MS.gene021069:CDS |
| CAATCTTATTGCCGTCAAAA+CGG | 0.544280 | 4.3:-40498282 | None:intergenic |
| TTATTGCCGTCAAAACGGTT+CGG | 0.545486 | 4.3:-40498277 | None:intergenic |
| CTTTGAATTGCAATCTTCCG+AGG | 0.557056 | 4.3:-40498804 | None:intergenic |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 4.3:+40498896 | MS.gene021069:CDS |
| TGAATGTGTAGCGTATTCCT+CGG | 0.575387 | 4.3:+40498787 | MS.gene021069:intron |
| AGAAATACGTTGGAAGTGGA+TGG | 0.575514 | 4.3:+40498919 | MS.gene021069:CDS |
| ATGGAGAAATACGTTGGAAG+TGG | 0.576439 | 4.3:+40498915 | MS.gene021069:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.580120 | 4.3:+40498646 | MS.gene021069:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.583531 | 4.3:+40498909 | MS.gene021069:CDS |
| CCATCTCAAAACGGCGACGA+TGG | 0.584489 | 4.3:-40498213 | None:intergenic |
| AGGGCAAAAGAACTCAAAGA+AGG | 0.584629 | 4.3:+40498954 | MS.gene021069:CDS |
| GGTATCAATTCGTCCAACTC+CGG | 0.591453 | 4.3:-40498533 | None:intergenic |
| GATACAAATAATTTGTGTGG+AGG | 0.593345 | 4.3:-40499005 | None:intergenic |
| ACTGATACAAATAATTTGTG+TGG | 0.607437 | 4.3:-40499008 | None:intergenic |
| CCAAATGAAGAAGAAGTCGA+TGG | 0.658231 | 4.3:+40498569 | MS.gene021069:CDS |
| GCGACTGATGAAATGTGCGG+CGG | 0.736322 | 4.3:+40498179 | MS.gene021069:CDS |
| ATGGCGACTGATGAAATGTG+CGG | 0.764233 | 4.3:+40498176 | MS.gene021069:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAATTTCAAAATTTTCAAAA+CGG | + | chr4.3:40498605-40498624 | MS.gene021069:CDS | 15.0% |
| !!! | GTTGGTTTTTATTTTATTTT+TGG | + | chr4.3:40498689-40498708 | MS.gene021069:intron | 15.0% |
| !!! | GTTTTTTATTTTATTTTTGC+TGG | + | chr4.3:40498711-40498730 | MS.gene021069:intron | 15.0% |
| !!! | TTGTTGTTTTTTTAATCATC+TGG | + | chr4.3:40498468-40498487 | MS.gene021069:intron | 20.0% |
| ! | CTTAAAATAGCCTTCTAAAT+GGG | - | chr4.3:40498350-40498369 | None:intergenic | 25.0% |
| !! | ACTGATACAAATAATTTGTG+TGG | - | chr4.3:40499011-40499030 | None:intergenic | 25.0% |
| !! | ATCATCAACAATTCGAATTT+TGG | - | chr4.3:40498847-40498866 | None:intergenic | 25.0% |
| !! | TTTGAAATTGTGCAAATACT+TGG | - | chr4.3:40498595-40498614 | None:intergenic | 25.0% |
| !!! | CATTTAGAAGGCTATTTTAA+GGG | + | chr4.3:40498349-40498368 | MS.gene021069:intron | 25.0% |
| AAGTTCGTGATAAAGGAAAT+TGG | + | chr4.3:40498892-40498911 | MS.gene021069:CDS | 30.0% | |
| CCTTAAAATAGCCTTCTAAA+TGG | - | chr4.3:40498351-40498370 | None:intergenic | 30.0% | |
| ! | AAAACGGTGATCATACTTTT+TGG | + | chr4.3:40498621-40498640 | MS.gene021069:CDS | 30.0% |
| ! | GATACAAATAATTTGTGTGG+AGG | - | chr4.3:40499008-40499027 | None:intergenic | 30.0% |
| !! | CCATTTAGAAGGCTATTTTA+AGG | + | chr4.3:40498348-40498367 | MS.gene021069:intron | 30.0% |
| AATTGGATGGAGAAATACGT+TGG | + | chr4.3:40498909-40498928 | MS.gene021069:CDS | 35.0% | |
| ACGAAGAAGAAGATGAAGAT+CGG | + | chr4.3:40498236-40498255 | MS.gene021069:CDS | 35.0% | |
| ACGTCTGAGAAGAAAATTCT+AGG | + | chr4.3:40498396-40498415 | MS.gene021069:intron | 35.0% | |
| CAATCTTATTGCCGTCAAAA+CGG | - | chr4.3:40498285-40498304 | None:intergenic | 35.0% | |
| CGTCTGAGAAGAAAATTCTA+GGG | + | chr4.3:40498397-40498416 | MS.gene021069:intron | 35.0% | |
| GAATGTGAAGTTCGTGATAA+AGG | + | chr4.3:40498885-40498904 | MS.gene021069:CDS | 35.0% | |
| TCGTGATAAAGGAAATTGGA+TGG | + | chr4.3:40498896-40498915 | MS.gene021069:CDS | 35.0% | |
| TGAAAATGTCTTCTTCTAGC+AGG | + | chr4.3:40498428-40498447 | MS.gene021069:intron | 35.0% | |
| TTAAGGGTTGTTGTGAATCT+TGG | + | chr4.3:40498365-40498384 | MS.gene021069:intron | 35.0% | |
| ! | TCATTTGGATTTTCGTCTAC+AGG | - | chr4.3:40498557-40498576 | None:intergenic | 35.0% |
| !! | CGAATTGTTGATGATGAAAC+TGG | + | chr4.3:40498852-40498871 | MS.gene021069:CDS | 35.0% |
| !! | GCATAGTATTAGTTCTGCTA+CGG | + | chr4.3:40498646-40498665 | MS.gene021069:CDS | 35.0% |
| !! | GTTTTGACGGCAATAAGATT+GGG | + | chr4.3:40498284-40498303 | MS.gene021069:CDS | 35.0% |
| !! | TGGATGGTTTGAGTTTGTTA+GGG | + | chr4.3:40498935-40498954 | MS.gene021069:CDS | 35.0% |
| !!! | TGGGCTTTTTAGTTATTGAC+CGG | + | chr4.3:40498303-40498322 | MS.gene021069:CDS | 35.0% |
| AGAAATACGTTGGAAGTGGA+TGG | + | chr4.3:40498919-40498938 | MS.gene021069:CDS | 40.0% | |
| AGGGCAAAAGAACTCAAAGA+AGG | + | chr4.3:40498954-40498973 | MS.gene021069:CDS | 40.0% | |
| ATGGAGAAATACGTTGGAAG+TGG | + | chr4.3:40498915-40498934 | MS.gene021069:CDS | 40.0% | |
| ATTGACCGGGCAAAAATGAA+TGG | + | chr4.3:40498317-40498336 | MS.gene021069:CDS | 40.0% | |
| CCAAATGAAGAAGAAGTCGA+TGG | + | chr4.3:40498569-40498588 | MS.gene021069:CDS | 40.0% | |
| CGAAGAAGAAGATGAAGATC+GGG | + | chr4.3:40498237-40498256 | MS.gene021069:CDS | 40.0% | |
| CTTCTTCGTCCATCTCAAAA+CGG | - | chr4.3:40498225-40498244 | None:intergenic | 40.0% | |
| CTTTGAATTGCAATCTTCCG+AGG | - | chr4.3:40498807-40498826 | None:intergenic | 40.0% | |
| GAAGATCGGGTCATTTGATT+GGG | + | chr4.3:40498250-40498269 | MS.gene021069:CDS | 40.0% | |
| TGAAGATCGGGTCATTTGAT+TGG | + | chr4.3:40498249-40498268 | MS.gene021069:CDS | 40.0% | |
| TGAATGTGTAGCGTATTCCT+CGG | + | chr4.3:40498787-40498806 | MS.gene021069:intron | 40.0% | |
| TTATTGCCGTCAAAACGGTT+CGG | - | chr4.3:40498280-40498299 | None:intergenic | 40.0% | |
| TTGACCGGGCAAAAATGAAT+GGG | + | chr4.3:40498318-40498337 | MS.gene021069:CDS | 40.0% | |
| ! | GTGGATGGTTTGAGTTTGTT+AGG | + | chr4.3:40498934-40498953 | MS.gene021069:CDS | 40.0% |
| ! | TTGCTGGAGTATTTGCACAT+GGG | + | chr4.3:40498727-40498746 | MS.gene021069:intron | 40.0% |
| ! | TTTGCTGGAGTATTTGCACA+TGG | + | chr4.3:40498726-40498745 | MS.gene021069:intron | 40.0% |
| !! | CCATCGACTTCTTCTTCATT+TGG | - | chr4.3:40498572-40498591 | None:intergenic | 40.0% |
| !! | CGTTTTGACGGCAATAAGAT+TGG | + | chr4.3:40498283-40498302 | MS.gene021069:CDS | 40.0% |
| !!! | GGGCTTTTTAGTTATTGACC+GGG | + | chr4.3:40498304-40498323 | MS.gene021069:CDS | 40.0% |
| ATGGCGACTGATGAAATGTG+CGG | + | chr4.3:40498176-40498195 | MS.gene021069:CDS | 45.0% | |
| CGATGTAATTGATCCGGAGT+TGG | + | chr4.3:40498520-40498539 | MS.gene021069:CDS | 45.0% | |
| CTGCAGCGATGTAATTGATC+CGG | + | chr4.3:40498514-40498533 | MS.gene021069:intron | 45.0% | |
| GGGCAAAAATGAATGGGTCA+CGG | + | chr4.3:40498324-40498343 | MS.gene021069:intron | 45.0% | |
| GGTATCAATTCGTCCAACTC+CGG | - | chr4.3:40498536-40498555 | None:intergenic | 45.0% | |
| ! | GGTTTTCCGAACCGTTTTGA+CGG | + | chr4.3:40498271-40498290 | MS.gene021069:CDS | 45.0% |
| !! | GTGACCCATTCATTTTTGCC+CGG | - | chr4.3:40498325-40498344 | None:intergenic | 45.0% |
| GAGTATTTGCACATGGGTGG+TGG | + | chr4.3:40498733-40498752 | MS.gene021069:intron | 50.0% | |
| ! | CTGGAGTATTTGCACATGGG+TGG | + | chr4.3:40498730-40498749 | MS.gene021069:intron | 50.0% |
| !! | ATTAGTTCTGCTACGGCTGC+TGG | + | chr4.3:40498653-40498672 | MS.gene021069:CDS | 50.0% |
| ATGAAATGTGCGGCGGATGC+AGG | + | chr4.3:40498186-40498205 | MS.gene021069:CDS | 55.0% | |
| CCATCTCAAAACGGCGACGA+TGG | - | chr4.3:40498216-40498235 | None:intergenic | 55.0% | |
| GCGACTGATGAAATGTGCGG+CGG | + | chr4.3:40498179-40498198 | MS.gene021069:CDS | 55.0% | |
| TGGGTCACGGCCCATTTAGA+AGG | + | chr4.3:40498337-40498356 | MS.gene021069:intron | 55.0% | |
| ! | GCTGGACGAAGAGTTCTGGT+TGG | + | chr4.3:40498671-40498690 | MS.gene021069:intron | 55.0% |
| !!! | CCATCGTCGCCGTTTTGAGA+TGG | + | chr4.3:40498213-40498232 | MS.gene021069:CDS | 55.0% |
| GGCTGCTGGACGAAGAGTTC+TGG | + | chr4.3:40498667-40498686 | MS.gene021069:CDS | 60.0% | |
| ! | GCACATGGGTGGTGGCTTTC+TGG | + | chr4.3:40498741-40498760 | MS.gene021069:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 40498176 | 40499040 | 40498176 | ID=MS.gene021069 |
| chr4.3 | mRNA | 40498176 | 40499040 | 40498176 | ID=MS.gene021069.t1;Parent=MS.gene021069 |
| chr4.3 | exon | 40498176 | 40498339 | 40498176 | ID=MS.gene021069.t1.exon1;Parent=MS.gene021069.t1 |
| chr4.3 | CDS | 40498176 | 40498339 | 40498176 | ID=cds.MS.gene021069.t1;Parent=MS.gene021069.t1 |
| chr4.3 | exon | 40498520 | 40498688 | 40498520 | ID=MS.gene021069.t1.exon2;Parent=MS.gene021069.t1 |
| chr4.3 | CDS | 40498520 | 40498688 | 40498520 | ID=cds.MS.gene021069.t1;Parent=MS.gene021069.t1 |
| chr4.3 | exon | 40498798 | 40499040 | 40498798 | ID=MS.gene021069.t1.exon3;Parent=MS.gene021069.t1 |
| chr4.3 | CDS | 40498798 | 40499040 | 40498798 | ID=cds.MS.gene021069.t1;Parent=MS.gene021069.t1 |
| Gene Sequence |
| Protein sequence |
