AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021108

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021108.t1 MTR_4g063735 95.349 258 7 3 1 254 1 257 0.0 500
MS.gene021108.t1 MTR_8g107210 56.383 94 35 2 12 99 80 173 7.75e-28 109
MS.gene021108.t1 MTR_4g084550 57.317 82 35 0 6 87 45 126 2.88e-27 103
MS.gene021108.t1 MTR_1g019130 62.500 72 27 0 20 91 85 156 6.33e-27 104
MS.gene021108.t1 MTR_1g019130 35.754 179 85 4 20 196 85 235 1.23e-26 103
MS.gene021108.t1 MTR_7g060630 66.667 66 22 0 23 88 6 71 6.74e-26 100
MS.gene021108.t1 MTR_5g021930 68.182 66 20 1 22 86 27 92 6.46e-25 100
MS.gene021108.t1 MTR_5g081990 51.899 79 37 1 13 90 20 98 3.81e-24 95.9
MS.gene021108.t1 MTR_8g095580 65.455 55 19 0 34 88 91 145 3.53e-20 84.7
MS.gene021108.t1 MTR_3g088485 63.636 55 20 0 34 88 89 143 7.07e-20 84.0
MS.gene021108.t1 MTR_2g015000 48.387 62 32 0 25 86 55 116 4.26e-14 71.6
MS.gene021108.t1 MTR_7g025010 45.161 62 34 0 25 86 47 108 2.30e-13 69.7
MS.gene021108.t1 MTR_1g115315 46.154 65 35 0 20 84 80 144 6.13e-13 67.0
MS.gene021108.t1 MTR_1g115315 44.776 67 37 0 20 86 80 146 7.51e-13 67.0
MS.gene021108.t1 MTR_4g088070 44.262 61 34 0 25 85 29 89 3.56e-12 65.9
MS.gene021108.t1 MTR_3g115620 40.909 66 39 0 20 85 77 142 1.08e-11 62.8
MS.gene021108.t1 MTR_7g023810 45.161 62 34 0 25 86 68 129 3.53e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021108.t1 AT5G59340 40.856 257 115 8 17 250 6 248 2.30e-53 173
MS.gene021108.t1 AT3G18010 45.312 128 57 2 20 134 71 198 5.71e-29 112
MS.gene021108.t1 AT1G46480 36.994 173 98 3 24 196 89 250 1.57e-26 103
MS.gene021108.t1 AT1G46480 65.672 67 23 0 24 90 89 155 3.49e-26 101
MS.gene021108.t1 AT2G28610 62.687 67 25 0 22 88 5 71 7.43e-25 99.4
MS.gene021108.t1 AT3G11260 50.000 80 40 0 13 92 12 91 8.15e-25 97.4
MS.gene021108.t1 AT5G05770 54.054 74 34 0 21 94 25 98 1.00e-24 95.5
MS.gene021108.t1 AT2G17950 63.636 66 23 1 22 86 35 100 2.24e-23 96.3
MS.gene021108.t1 AT2G01500 64.407 59 21 0 21 79 57 115 5.53e-22 92.4
MS.gene021108.t1 AT5G45980 51.613 62 30 0 25 86 55 116 7.88e-15 73.2
MS.gene021108.t1 AT1G20700 40.506 79 47 0 13 91 21 99 8.10e-15 70.1
MS.gene021108.t1 AT1G20700 40.506 79 47 0 13 91 83 161 1.85e-14 70.5
MS.gene021108.t1 AT1G20700 40.506 79 47 0 13 91 83 161 1.92e-14 70.5
MS.gene021108.t1 AT2G33880 48.387 62 32 0 25 86 55 116 9.26e-14 70.5
MS.gene021108.t1 AT2G33880 48.387 62 32 0 25 86 55 116 9.41e-14 70.5
MS.gene021108.t1 AT4G35550 40.278 72 43 0 14 85 88 159 3.00e-13 68.2
MS.gene021108.t1 AT1G20710 50.000 54 27 0 32 85 86 139 2.04e-11 62.0

Find 46 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATAGGTGAAGGATAAA+TGG 0.223363 4.3:+40021726 None:intergenic
AATCAAGATCATGGTTATAA+TGG 0.248654 4.3:-40020480 MS.gene021108:CDS
CTGTAACCAAAGTTATAGTT+AGG 0.305798 4.3:+40020648 None:intergenic
TTCTGCAGGACAAGGTTCTT+AGG 0.334683 4.3:-40020427 None:intergenic
AGTTCCACTGATACACATTC+TGG 0.341293 4.3:-40020525 MS.gene021108:CDS
AACATACCATTTGGACAAAT+AGG 0.347632 4.3:+40021712 None:intergenic
ACTGCATCCAACTGGCATAT+TGG 0.347683 4.3:-40020598 MS.gene021108:CDS
ACTGCAGGTAGAAGCACTTT+TGG 0.360138 4.3:+40020771 None:intergenic
TAGCTTATTTCATGACTTTG+TGG 0.366655 4.3:+40020807 None:intergenic
CCTTCACCTATTTGTCCAAA+TGG 0.376093 4.3:-40021718 MS.gene021108:intron
ATCAAGATCATGGTTATAAT+GGG 0.392817 4.3:-40020479 MS.gene021108:CDS
ATTAATGAGGAGGGTTCAAA+TGG 0.414428 4.3:-40022006 MS.gene021108:CDS
TGTGGTTGTGGTACGCAATA+TGG 0.424892 4.3:+40020825 None:intergenic
ATTTCATGACTTTGTGGTTG+TGG 0.425330 4.3:+40020813 None:intergenic
CAAATAGGTGAAGGATAAAT+GGG 0.425469 4.3:+40021727 None:intergenic
TCTTGTATTTCTTCAGCACT+TGG 0.437582 4.3:+40021892 None:intergenic
TTGTTTCCACTGCATCCAAC+TGG 0.438638 4.3:-40020606 MS.gene021108:CDS
AGAATCCCTAACTATAACTT+TGG 0.443156 4.3:-40020654 MS.gene021108:CDS
TTCCCTTCCAATATGCCAGT+TGG 0.473137 4.3:+40020591 None:intergenic
GCTGAGAAAGTTATGTCAAA+TGG 0.482284 4.3:-40020729 MS.gene021108:CDS
ACTGCTAGGCTTAGAGTGTA+TGG 0.483688 4.3:-40021865 MS.gene021108:CDS
TGTAACCAAAGTTATAGTTA+GGG 0.484125 4.3:+40020649 None:intergenic
TTGGAGAATTTGTATAAGCA+AGG 0.498360 4.3:-40021925 MS.gene021108:CDS
CATGATATCAATCAAGATCA+TGG 0.502262 4.3:-40020489 MS.gene021108:CDS
AAAAGAACAAATAAGTATGT+TGG 0.504717 4.3:-40021944 MS.gene021108:CDS
CATCCAACTGGCATATTGGA+AGG 0.509666 4.3:-40020594 MS.gene021108:CDS
TTACAGTAATCAAGAAACAT+TGG 0.520782 4.3:-40020631 MS.gene021108:CDS
GGTGAAGGATAAATGGGTTG+AGG 0.539516 4.3:+40021733 None:intergenic
TTTAATAATTCTGCAGGACA+AGG 0.556573 4.3:-40020435 MS.gene021108:CDS
TTCCTTCTAAAACCTACTGC+AGG 0.572590 4.3:+40020756 None:intergenic
AATAATGAGATTAATGAGGA+GGG 0.573895 4.3:-40022015 MS.gene021108:CDS
AGAAGAAGAAGAATAATGGA+GGG 0.580379 4.3:-40022045 None:intergenic
CTGAAACAATAGAAGACACC+TGG 0.584011 4.3:+40020559 None:intergenic
TAATAATGAGATTAATGAGG+AGG 0.585231 4.3:-40022016 MS.gene021108:CDS
AAAGTGCTTCTACCTGCAGT+AGG 0.586289 4.3:-40020768 MS.gene021108:CDS
AATATGCCAGTTGGATGCAG+TGG 0.586423 4.3:+40020600 None:intergenic
TTAATGAGGAGGGTTCAAAT+GGG 0.588886 4.3:-40022005 MS.gene021108:CDS
AGAACCAGAATGTGTATCAG+TGG 0.599607 4.3:+40020521 None:intergenic
AGAGTGTATGGTCACATCGA+AGG 0.601558 4.3:-40021853 MS.gene021108:CDS
AAATATCTGCAATAGTCCAA+TGG 0.604277 4.3:-40020700 MS.gene021108:CDS
CCATTTGGACAAATAGGTGA+AGG 0.604508 4.3:+40021718 None:intergenic
AAATACAAGAAATCACTGCT+AGG 0.611419 4.3:-40021879 MS.gene021108:CDS
GAATAATAATGAGATTAATG+AGG 0.611694 4.3:-40022019 MS.gene021108:CDS
ATCCAACTGGCATATTGGAA+GGG 0.612491 4.3:-40020593 MS.gene021108:CDS
GGAAGGGAAAACAACTGACC+AGG 0.627194 4.3:-40020577 MS.gene021108:CDS
GTATTTCATCAAGTTCACGA+TGG 0.702362 4.3:-40021975 MS.gene021108:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACTAATAGTTAAAAACA+AGG + chr4.3:40021128-40021147 None:intergenic 15.0%
!! ATTATTAAAAAAGTCAAAGA+AGG + chr4.3:40022012-40022031 None:intergenic 15.0%
!! TTATTAAAAAAGTCAAAGAA+GGG + chr4.3:40022011-40022030 None:intergenic 15.0%
!!! AACTAAAAAAGCATTAAAAA+AGG - chr4.3:40021437-40021456 MS.gene021108:intron 15.0%
!!! GGTAATTGAATTATTATTTT+TGG - chr4.3:40021592-40021611 MS.gene021108:intron 15.0%
!!! TTGTTCTTATTATATTTTCA+TGG - chr4.3:40020843-40020862 MS.gene021108:intron 15.0%
!! AAAAGAACAAATAAGTATGT+TGG - chr4.3:40020514-40020533 MS.gene021108:CDS 20.0%
!! ACCAAAAATAAAATACTAGA+AGG + chr4.3:40021317-40021336 None:intergenic 20.0%
!! ATCTATATTGTGGTAAAATA+TGG - chr4.3:40021072-40021091 MS.gene021108:intron 20.0%
!! CAAAAACATTGATATGAAAA+TGG + chr4.3:40020826-40020845 None:intergenic 20.0%
!! GAATAATAATGAGATTAATG+AGG - chr4.3:40020439-40020458 MS.gene021108:CDS 20.0%
!! TCTATATTGTGGTAAAATAT+GGG - chr4.3:40021073-40021092 MS.gene021108:intron 20.0%
!! TGAAAATGAGATTTATTGAA+AGG + chr4.3:40021225-40021244 None:intergenic 20.0%
!!! ATACTTATTTGTTCTTTTGT+TGG + chr4.3:40020512-40020531 None:intergenic 20.0%
!!! ATGTTTTCATTATATACCAT+TGG + chr4.3:40021777-40021796 None:intergenic 20.0%
!!! TACTTATTTGTTCTTTTGTT+GGG + chr4.3:40020511-40020530 None:intergenic 20.0%
!!! TTCAATAAATCTCATTTTCA+TGG - chr4.3:40021225-40021244 MS.gene021108:intron 20.0%
! AAGGTTAATTTCTCCTAAAA+CGG + chr4.3:40021206-40021225 None:intergenic 25.0%
! AATAATGAGATTAATGAGGA+GGG - chr4.3:40020443-40020462 MS.gene021108:CDS 25.0%
! AATCAAGATCATGGTTATAA+TGG - chr4.3:40021978-40021997 MS.gene021108:CDS 25.0%
! ATCAAGATCATGGTTATAAT+GGG - chr4.3:40021979-40021998 MS.gene021108:CDS 25.0%
! CAAATAAATTACTCCATCTT+TGG + chr4.3:40021486-40021505 None:intergenic 25.0%
! TAAAACGGGATTTATATAGA+GGG + chr4.3:40021191-40021210 None:intergenic 25.0%
! TAATAATGAGATTAATGAGG+AGG - chr4.3:40020442-40020461 MS.gene021108:CDS 25.0%
! TATAACCTAGAACTAGAAAA+AGG + chr4.3:40020783-40020802 None:intergenic 25.0%
! TATCTTTGTACGACATAAAA+TGG - chr4.3:40020968-40020987 MS.gene021108:intron 25.0%
! TGTAACCAAAGTTATAGTTA+GGG + chr4.3:40021812-40021831 None:intergenic 25.0%
! TTACAGTAATCAAGAAACAT+TGG - chr4.3:40021827-40021846 MS.gene021108:CDS 25.0%
!!! GACTTTTTTAATAATTCTGC+AGG - chr4.3:40022017-40022036 MS.gene021108:CDS 25.0%
!!! GCCTTCTAGTATTTTATTTT+TGG - chr4.3:40021313-40021332 MS.gene021108:intron 25.0%
!!! TTGTTGTTGTATCTTTTTCA+TGG - chr4.3:40021038-40021057 MS.gene021108:intron 25.0%
AAATACAAGAAATCACTGCT+AGG - chr4.3:40020579-40020598 MS.gene021108:CDS 30.0%
AAATATCTGCAATAGTCCAA+TGG - chr4.3:40021758-40021777 MS.gene021108:CDS 30.0%
AACAGAAACATAACCAAAGA+TGG - chr4.3:40021470-40021489 MS.gene021108:intron 30.0%
AACATACCATTTGGACAAAT+AGG + chr4.3:40020749-40020768 None:intergenic 30.0%
ACAAATAGGTGAAGGATAAA+TGG + chr4.3:40020735-40020754 None:intergenic 30.0%
AGAATCCCTAACTATAACTT+TGG - chr4.3:40021804-40021823 MS.gene021108:CDS 30.0%
AGGTTAATTTCTCCTAAAAC+GGG + chr4.3:40021205-40021224 None:intergenic 30.0%
CAAATAGGTGAAGGATAAAT+GGG + chr4.3:40020734-40020753 None:intergenic 30.0%
CATGATATCAATCAAGATCA+TGG - chr4.3:40021969-40021988 MS.gene021108:CDS 30.0%
CCATATTTAAATTGTGCTAG+CGG - chr4.3:40020909-40020928 MS.gene021108:intron 30.0%
CTAAAACGGGATTTATATAG+AGG + chr4.3:40021192-40021211 None:intergenic 30.0%
CTGTAACCAAAGTTATAGTT+AGG + chr4.3:40021813-40021832 None:intergenic 30.0%
TAGCTTATTTCATGACTTTG+TGG + chr4.3:40021654-40021673 None:intergenic 30.0%
TTCTGCAAACATCTATATTG+TGG - chr4.3:40021062-40021081 MS.gene021108:intron 30.0%
TTGGAGAATTTGTATAAGCA+AGG - chr4.3:40020533-40020552 MS.gene021108:CDS 30.0%
TTTAATAATTCTGCAGGACA+AGG - chr4.3:40022023-40022042 MS.gene021108:CDS 30.0%
TTTCTTCATGCAAATGTGTT+TGG - chr4.3:40021375-40021394 MS.gene021108:intron 30.0%
! AAAACAAGGTGCATTTTCAA+CGG + chr4.3:40021114-40021133 None:intergenic 30.0%
! AACCTAATGAGTACTACTTT+TGG + chr4.3:40020884-40020903 None:intergenic 30.0%
! AAGGAAAGAATGTCTTTTAC+TGG - chr4.3:40020624-40020643 MS.gene021108:CDS 30.0%
! CTCTATATAAATCCCGTTTT+AGG - chr4.3:40021190-40021209 MS.gene021108:intron 30.0%
!!! TTCTTCCTTTTTCTAGTTCT+AGG - chr4.3:40020775-40020794 MS.gene021108:CDS 30.0%
ATTAATGAGGAGGGTTCAAA+TGG - chr4.3:40020452-40020471 MS.gene021108:CDS 35.0%
ATTTCATGACTTTGTGGTTG+TGG + chr4.3:40021648-40021667 None:intergenic 35.0%
CCGCTAGCACAATTTAAATA+TGG + chr4.3:40020912-40020931 None:intergenic 35.0%
CTAGTATTCTACTGTAACGT+TGG - chr4.3:40021571-40021590 MS.gene021108:intron 35.0%
GAAGTTGAGAACATACCATT+TGG + chr4.3:40020758-40020777 None:intergenic 35.0%
GCTGAGAAAGTTATGTCAAA+TGG - chr4.3:40021729-40021748 MS.gene021108:CDS 35.0%
GTATTTCATCAAGTTCACGA+TGG - chr4.3:40020483-40020502 MS.gene021108:CDS 35.0%
TCTTGTATTTCTTCAGCACT+TGG + chr4.3:40020569-40020588 None:intergenic 35.0%
TGCCAAAAGTAGTACTCATT+AGG - chr4.3:40020879-40020898 MS.gene021108:intron 35.0%
TTAATGAGGAGGGTTCAAAT+GGG - chr4.3:40020453-40020472 MS.gene021108:CDS 35.0%
!! GAAGCACTTTTGGATTTTGA+GGG + chr4.3:40021680-40021699 None:intergenic 35.0%
!!! AGAAGCACTTTTGGATTTTG+AGG + chr4.3:40021681-40021700 None:intergenic 35.0%
AGAACCAGAATGTGTATCAG+TGG + chr4.3:40021940-40021959 None:intergenic 40.0%
AGTTCCACTGATACACATTC+TGG - chr4.3:40021933-40021952 MS.gene021108:CDS 40.0%
ATCCAACTGGCATATTGGAA+GGG - chr4.3:40021865-40021884 MS.gene021108:CDS 40.0%
CCATTTGGACAAATAGGTGA+AGG + chr4.3:40020743-40020762 None:intergenic 40.0%
CCTTCACCTATTTGTCCAAA+TGG - chr4.3:40020740-40020759 MS.gene021108:CDS 40.0%
CTGAAACAATAGAAGACACC+TGG + chr4.3:40021902-40021921 None:intergenic 40.0%
!! TTCCTTCTAAAACCTACTGC+AGG + chr4.3:40021705-40021724 None:intergenic 40.0%
!!! TACCTGCAGTAGGTTTTAGA+AGG - chr4.3:40021700-40021719 MS.gene021108:intron 40.0%
AATATGCCAGTTGGATGCAG+TGG + chr4.3:40021861-40021880 None:intergenic 45.0%
ACTGCATCCAACTGGCATAT+TGG - chr4.3:40021860-40021879 MS.gene021108:CDS 45.0%
ACTGCTAGGCTTAGAGTGTA+TGG - chr4.3:40020593-40020612 MS.gene021108:CDS 45.0%
AGAGTGTATGGTCACATCGA+AGG - chr4.3:40020605-40020624 MS.gene021108:CDS 45.0%
CATCCAACTGGCATATTGGA+AGG - chr4.3:40021864-40021883 MS.gene021108:CDS 45.0%
TGTGGTTGTGGTACGCAATA+TGG + chr4.3:40021636-40021655 None:intergenic 45.0%
TTCCCTTCCAATATGCCAGT+TGG + chr4.3:40021870-40021889 None:intergenic 45.0%
TTGTTTCCACTGCATCCAAC+TGG - chr4.3:40021852-40021871 MS.gene021108:CDS 45.0%
! AAAGTGCTTCTACCTGCAGT+AGG - chr4.3:40021690-40021709 MS.gene021108:intron 45.0%
!! AAATTGTGCTAGCGGACTTG+TGG - chr4.3:40020917-40020936 MS.gene021108:intron 45.0%
!! GGTGAAGGATAAATGGGTTG+AGG + chr4.3:40020728-40020747 None:intergenic 45.0%
!!! ACTGCAGGTAGAAGCACTTT+TGG + chr4.3:40021690-40021709 None:intergenic 45.0%
GGAAGGGAAAACAACTGACC+AGG - chr4.3:40021881-40021900 MS.gene021108:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 40020428 40022052 40020428 ID=MS.gene021108
chr4.3 mRNA 40020428 40022052 40020428 ID=MS.gene021108.t1;Parent=MS.gene021108
chr4.3 exon 40021719 40022052 40021719 ID=MS.gene021108.t1.exon1;Parent=MS.gene021108.t1
chr4.3 CDS 40021719 40022052 40021719 ID=cds.MS.gene021108.t1;Parent=MS.gene021108.t1
chr4.3 exon 40020428 40020858 40020428 ID=MS.gene021108.t1.exon2;Parent=MS.gene021108.t1
chr4.3 CDS 40020428 40020858 40020428 ID=cds.MS.gene021108.t1;Parent=MS.gene021108.t1
Gene Sequence

>MS.gene021108

ATGGAGGGTGAGAATAATAATGAGATTAATGAGGAGGGTTCAAATGGGAATAATAGTATTTCATCAAGTTCACGATGGAACCCAACAAAAGAACAAATAAGTATGTTGGAGAATTTGTATAAGCAAGGAATAAAGACACCAAGTGCTGAAGAAATACAAGAAATCACTGCTAGGCTTAGAGTGTATGGTCACATCGAAGGAAAGAATGTCTTTTACTGGTTTCAGAATCACAAAGCTAGACAGAGACAGAAACAGAAGCAAGAAAGTATTGCTTATTTCAATCGACTTCTTCATAGACCTCAACCCATTTATCCTTCACCTATTTGTCCAAATGGTATGTTCTCAACTTCTTCCTTTTTCTAGTTCTAGGTTATAAATTTTACATATACTTTATTCCATTTTCATATCAATGTTTTTGTTCTTATTATATTTTCATGGAAAAAAATTTCTATGCCAAAAGTAGTACTCATTAGGTTTTCAACCATATTTAAATTGTGCTAGCGGACTTGTGGTTATGCTGCAAACTTTTTAATTTTTTTTTATCTTTGTACGACATAAAATGGTTTATAATAGAATATTATGATGTAATTTGATTCATGTAGCGAACTTGTTGTTGTTGTATCTTTTTCATGGATTCTGCAAACATCTATATTGTGGTAAAATATGGGAAGATGCAGCTAATTCCGTTGAAAATGCACCTTGTTTTTAACTATTAGTTATTTGTTACATGTTAATTCAATCTCAGTTTAGAAGACATTAGCCCTCTATATAAATCCCGTTTTAGGAGAAATTAACCTTTCAATAAATCTCATTTTCATGGAAATTAATTTCTGTAATTTTTTATTTCAAATAAGTTAATTAGTTTATTCATAATTATCTTTTAATGCCTTCTAGTATTTTATTTTTGGTATTTAGTTGATTAAATATTTCTTGATATATTTTTTGCGTTTCTTCATGCAAATGTGTTTGGTGTATGTGTTCATAAAAAAGATTAACAAATCAAGATCTTAACTAAAAAAGCATTAAAAAAGGATAACTTCAAAACAGAAACATAACCAAAGATGGAGTAATTTATTTGCTGTGTGTTTTTGTTACAAATTAAATATACTTTTTAATCTAATAAAGTGTTTTTTTTTACACATTCTAGTATTCTACTGTAACGTTGGTAATTGAATTATTATTTTTGGTTTTCAGCTATCTGTGCACCATATTGCGTACCACAACCACAAAGTCATGAAATAAGCTATTACCCTCAAAATCCAAAAGTGCTTCTACCTGCAGTAGGTTTTAGAAGGAATCACGCTGAGAAAGTTATGTCAAATGGCATGTCAAATATCTGCAATAGTCCAATGGTATATAATGAAAACATGCAACAGAGAATCCCTAACTATAACTTTGGTTACAGTAATCAAGAAACATTGGATTTGTTTCCACTGCATCCAACTGGCATATTGGAAGGGAAAACAACTGACCAGGTGTCTTCTATTGTTTCAGTTTCTGCTGATAGTTCCACTGATACACATTCTGGTTCTTCTCATCATCATGATATCAATCAAGATCATGGTTATAATGGGAACAAACCCTTCTTTGACTTTTTTAATAATTCTGCAGGACAAGGTTCTTAG
Protein sequence

>MS.gene021108.t1

MEGENNNEINEEGSNGNNSISSSSRWNPTKEQISMLENLYKQGIKTPSAEEIQEITARLRVYGHIEGKNVFYWFQNHKARQRQKQKQESIAYFNRLLHRPQPIYPSPICPNAICAPYCVPQPQSHEISYYPQNPKVLLPAVGFRRNHAEKVMSNGMSNICNSPMVYNENMQQRIPNYNFGYSNQETLDLFPLHPTGILEGKTTDQVSSIVSVSADSSTDTHSGSSHHHDINQDHGYNGNKPFFDFFNNSAGQGS