AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021136

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021136.t1 MTR_4g064150 95.102 735 36 0 1 735 1 735 0.0 1421
MS.gene021136.t1 MTR_4g064120 72.139 664 135 7 73 735 14 628 0.0 933
MS.gene021136.t1 MTR_4g064180 71.837 664 137 8 73 735 14 628 0.0 922
MS.gene021136.t1 MTR_2g097410 56.178 785 252 18 1 735 1 743 0.0 816
MS.gene021136.t1 MTR_4g064160 49.280 694 278 14 83 735 26 686 0.0 609
MS.gene021136.t1 MTR_7g062120 45.152 691 329 17 83 735 64 742 0.0 546
MS.gene021136.t1 MTR_2g097467 44.611 668 296 18 86 735 45 656 9.06e-176 518
MS.gene021136.t1 MTR_4g064200 52.446 511 219 9 239 735 152 652 1.48e-173 513
MS.gene021136.t1 MTR_2g097473 44.595 666 299 17 86 735 45 656 2.58e-172 509
MS.gene021136.t1 MTR_2g097463 43.451 649 311 13 96 735 56 657 1.13e-167 498
MS.gene021136.t1 MTR_2g097310 48.326 478 230 7 260 735 178 640 1.04e-157 471
MS.gene021136.t1 MTR_2g097350 50.569 439 201 7 299 735 218 642 3.69e-151 454
MS.gene021136.t1 MTR_2g097390 47.817 458 220 8 283 735 200 643 7.99e-149 449
MS.gene021136.t1 MTR_2g097380 45.295 457 189 8 283 735 164 563 8.62e-131 399
MS.gene021136.t1 MTR_2g082090 32.979 376 247 4 357 732 208 578 7.65e-68 234
MS.gene021136.t1 MTR_5g097480 32.181 376 250 3 357 732 173 543 2.26e-63 221
MS.gene021136.t1 MTR_3g056110 32.804 378 245 6 357 732 171 541 9.98e-61 214
MS.gene021136.t1 MTR_2g026250 29.894 378 259 4 356 732 223 595 4.79e-59 210
MS.gene021136.t1 MTR_4g133660 30.829 386 247 8 356 733 170 543 1.81e-57 205
MS.gene021136.t1 MTR_5g094450 31.865 386 242 9 357 732 157 531 9.65e-57 202
MS.gene021136.t1 MTR_8g442410 30.500 400 242 7 353 735 147 527 4.25e-54 195
MS.gene021136.t1 MTR_1g029420 28.877 374 261 3 359 732 223 591 1.47e-53 195
MS.gene021136.t1 MTR_7g027190 31.281 406 232 8 351 732 290 672 1.75e-53 196
MS.gene021136.t1 MTR_3g089055 28.877 374 261 3 359 732 196 564 1.76e-53 194
MS.gene021136.t1 MTR_3g089055 28.877 374 261 3 359 732 201 569 1.76e-53 194
MS.gene021136.t1 MTR_4g104020 29.899 398 248 8 350 732 138 519 4.88e-53 192
MS.gene021136.t1 MTR_3g065980 30.435 414 251 8 336 732 146 539 4.84e-50 184
MS.gene021136.t1 MTR_7g074650 30.270 370 240 7 365 734 442 793 2.33e-46 177
MS.gene021136.t1 MTR_4g076140 27.293 458 261 9 337 732 21 468 3.15e-45 169
MS.gene021136.t1 MTR_2g034260 29.899 398 249 9 351 732 200 583 3.83e-44 168
MS.gene021136.t1 MTR_2g034250 29.873 395 254 9 351 732 200 584 4.33e-43 165
MS.gene021136.t1 MTR_5g009080 27.356 435 246 10 358 732 53 477 4.74e-41 157
MS.gene021136.t1 MTR_3g022830 26.366 421 249 8 362 732 26 435 1.35e-40 155
MS.gene021136.t1 MTR_4g102790 26.804 388 262 10 357 734 288 663 1.53e-39 155
MS.gene021136.t1 MTR_4g076020 27.224 371 243 5 365 735 88 431 3.76e-39 150
MS.gene021136.t1 MTR_4g097080 28.312 385 256 10 359 734 131 504 5.56e-38 149
MS.gene021136.t1 MTR_2g089100 26.650 409 257 9 358 735 49 445 1.09e-37 147
MS.gene021136.t1 MTR_2g034280 27.990 393 261 9 351 732 193 574 3.41e-36 144
MS.gene021136.t1 MTR_1g069725 27.763 389 245 14 353 732 101 462 5.99e-36 142
MS.gene021136.t1 MTR_1g096030 30.250 400 236 14 358 732 77 458 1.16e-34 138
MS.gene021136.t1 MTR_3g072710 26.799 403 263 10 350 732 109 499 2.63e-33 135
MS.gene021136.t1 MTR_5g015490 25.916 382 273 5 360 734 113 491 1.69e-32 132
MS.gene021136.t1 MTR_4g077760 27.198 364 239 9 377 732 209 554 2.72e-32 132
MS.gene021136.t1 MTR_5g058860 28.499 393 246 12 362 733 119 497 1.50e-30 127
MS.gene021136.t1 MTR_5g015950 23.709 426 268 11 358 735 32 448 1.62e-30 125
MS.gene021136.t1 MTR_3g053270 23.709 426 268 11 358 735 32 448 1.62e-30 125
MS.gene021136.t1 MTR_7g109580 28.205 390 257 12 351 732 179 553 1.87e-30 127
MS.gene021136.t1 MTR_7g057230 25.069 363 251 7 377 733 310 657 1.91e-30 128
MS.gene021136.t1 MTR_4g095500 24.582 419 271 9 358 735 56 470 3.04e-30 125
MS.gene021136.t1 MTR_6g047750 26.289 388 253 12 359 732 293 661 1.50e-29 125
MS.gene021136.t1 MTR_1g086970 26.434 401 257 13 351 732 96 477 7.20e-29 121
MS.gene021136.t1 MTR_8g093070 26.895 409 246 17 346 735 124 498 3.40e-28 119
MS.gene021136.t1 MTR_2g099110 25.667 300 213 4 361 659 74 364 1.12e-21 99.4
MS.gene021136.t1 MTR_0092s0100 22.475 396 286 7 338 732 355 730 2.89e-15 80.1
MS.gene021136.t1 MTR_0092s0100 22.475 396 286 7 338 732 352 727 3.06e-15 80.1
MS.gene021136.t1 MTR_8g020840 24.352 386 233 14 361 719 171 524 1.46e-14 77.8
MS.gene021136.t1 MTR_4g026485 24.000 375 260 9 362 732 270 623 2.08e-14 77.4
MS.gene021136.t1 MTR_3g087300 44.000 75 32 2 316 380 134 208 6.59e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021136.t1 AT1G07530 47.286 700 289 17 96 735 88 767 0.0 578
MS.gene021136.t1 AT2G29060 47.793 657 280 15 88 734 53 656 0.0 562
MS.gene021136.t1 AT2G29060 47.605 668 302 14 88 734 53 693 0.0 558
MS.gene021136.t1 AT2G29065 45.776 651 283 15 96 735 39 630 0.0 535
MS.gene021136.t1 AT1G07520 46.296 648 317 11 96 735 71 695 2.58e-180 531
MS.gene021136.t1 AT1G07520 46.296 648 317 11 96 735 72 696 2.84e-180 531
MS.gene021136.t1 AT1G07520 51.136 528 240 8 214 735 16 531 5.58e-179 522
MS.gene021136.t1 AT1G07520 51.136 528 240 8 214 735 16 531 5.58e-179 522
MS.gene021136.t1 AT2G37650 43.639 676 326 18 83 734 58 702 1.10e-167 499
MS.gene021136.t1 AT2G37650 43.639 676 326 18 83 734 70 714 1.80e-167 499
MS.gene021136.t1 AT3G46600 50.111 451 208 5 285 735 148 581 1.37e-152 456
MS.gene021136.t1 AT3G46600 50.111 451 208 5 285 735 116 549 2.15e-152 454
MS.gene021136.t1 AT5G59450 47.708 480 232 7 264 735 132 600 4.59e-149 448
MS.gene021136.t1 AT3G46600 52.910 378 173 2 358 735 1 373 1.66e-141 420
MS.gene021136.t1 AT2G04890 32.181 376 243 4 357 732 49 412 2.20e-60 209
MS.gene021136.t1 AT1G50600 32.181 376 250 3 357 732 155 525 5.96e-60 211
MS.gene021136.t1 AT1G50600 32.181 376 250 3 357 732 226 596 2.79e-59 211
MS.gene021136.t1 AT1G50600 32.181 376 250 3 357 732 230 600 3.67e-59 211
MS.gene021136.t1 AT5G48150 30.585 376 257 3 357 732 118 489 1.31e-57 204
MS.gene021136.t1 AT5G48150 30.585 376 257 3 357 732 118 489 1.31e-57 204
MS.gene021136.t1 AT5G48150 30.585 376 257 3 357 732 118 489 1.31e-57 204
MS.gene021136.t1 AT5G48150 30.585 376 257 3 357 732 118 489 1.31e-57 204
MS.gene021136.t1 AT1G21450 30.585 376 253 5 359 732 223 592 4.39e-55 199
MS.gene021136.t1 AT4G17230 30.918 414 276 5 325 734 119 526 4.61e-53 192
MS.gene021136.t1 AT4G17230 30.918 414 276 5 325 734 119 526 4.61e-53 192
MS.gene021136.t1 AT1G66350 31.234 381 233 10 356 732 150 505 4.04e-48 178
MS.gene021136.t1 AT1G50420 25.694 432 257 5 358 732 54 478 5.35e-47 174
MS.gene021136.t1 AT2G01570 30.208 384 239 10 356 732 219 580 1.07e-46 176
MS.gene021136.t1 AT3G54220 30.280 393 257 8 343 734 275 651 1.12e-46 176
MS.gene021136.t1 AT3G03450 28.721 383 251 9 356 732 178 544 3.13e-46 173
MS.gene021136.t1 AT1G14920 30.366 382 241 9 356 732 167 528 3.74e-46 173
MS.gene021136.t1 AT5G17490 26.190 420 280 10 320 732 119 515 1.45e-42 162
MS.gene021136.t1 AT5G41920 28.346 381 252 6 355 732 37 399 1.16e-41 157
MS.gene021136.t1 AT5G66770 26.914 405 278 8 333 732 192 583 2.11e-37 148
MS.gene021136.t1 AT5G52510 26.410 390 255 10 359 732 266 639 1.60e-36 146
MS.gene021136.t1 AT1G63100 31.231 333 215 8 357 686 273 594 1.80e-36 146
MS.gene021136.t1 AT1G63100 31.231 333 215 8 357 686 248 569 2.69e-36 145
MS.gene021136.t1 AT4G37650 25.445 393 268 10 360 734 145 530 1.68e-34 139
MS.gene021136.t1 AT3G50650 29.283 321 208 5 370 684 184 491 1.03e-33 137
MS.gene021136.t1 AT3G49950 26.854 391 264 10 358 734 27 409 2.23e-30 124
MS.gene021136.t1 AT1G55580 25.060 419 255 11 358 732 41 444 5.74e-26 112
MS.gene021136.t1 AT4G08250 26.700 397 261 14 351 732 95 476 1.04e-25 112
MS.gene021136.t1 AT3G13840 23.256 430 254 16 331 732 128 509 5.13e-15 79.0
MS.gene021136.t1 AT4G00150 24.403 377 246 12 363 732 209 553 2.22e-11 67.4

Find 167 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATTAATGGACAAATTT+AGG 0.087513 4.4:-41931747 MS.gene021136:CDS
GATGAGATTTAAGAATTTAT+TGG 0.142030 4.4:-41932118 MS.gene021136:CDS
ACCCTCCATATCATTGATTT+TGG 0.161875 4.4:-41932414 MS.gene021136:CDS
TCCATTGAATTCGGAATTAA+TGG 0.174492 4.4:-41931758 MS.gene021136:CDS
AATCAATATGATAGCTTTCT+TGG 0.191887 4.4:-41933656 MS.gene021136:CDS
ATGCCATACCATCGCGTTAT+TGG 0.192133 4.4:-41932203 MS.gene021136:CDS
CATTCTTTGCTACACGGTTT+AGG 0.223208 4.4:-41931987 MS.gene021136:CDS
AAATGCAGAACCGCATTTCT+AGG 0.241890 4.4:+41932072 None:intergenic
CTCCCTAACCATATCCAAAT+TGG 0.268685 4.4:+41933061 None:intergenic
TTTGTTCAGTCAATTGTTAA+TGG 0.288838 4.4:-41932024 MS.gene021136:CDS
ATCAGAGTTATGAATATCTA+AGG 0.292408 4.4:+41933391 None:intergenic
TGAATCATCCAATTTGGATA+TGG 0.301149 4.4:-41933069 MS.gene021136:CDS
CAAACAGCTTCCATTGAATT+CGG 0.304465 4.4:-41931767 MS.gene021136:CDS
ATTCTTTGCTACACGGTTTA+GGG 0.306681 4.4:-41931986 MS.gene021136:CDS
TATCTAAGGGGTGTTGATTA+GGG 0.313284 4.4:+41933405 None:intergenic
TCACTAGCTTGAGGAAAGTT+TGG 0.320173 4.4:+41933809 None:intergenic
ATATCTAAGGGGTGTTGATT+AGG 0.323460 4.4:+41933404 None:intergenic
CATCCAATTTGGATATGGTT+AGG 0.330014 4.4:-41933064 MS.gene021136:CDS
TGCCATACCATCGCGTTATT+GGG 0.330847 4.4:-41932202 MS.gene021136:CDS
ACTCTATCCCGGTGATCTTC+AGG 0.333841 4.4:+41932322 None:intergenic
GGATACCAAAATCAATGATA+TGG 0.351602 4.4:+41932409 None:intergenic
CGAGTCTTCCCTCCATTTGA+TGG 0.357746 4.4:+41932804 None:intergenic
AGAAAGCTATCATATTGATT+TGG 0.360772 4.4:+41933659 None:intergenic
AGGAAGCTATCATATTGATT+TGG 0.360772 4.4:+41933725 None:intergenic
TTTATATGCTTCCACATGTT+GGG 0.369926 4.4:-41931641 MS.gene021136:CDS
CATATAAACAGTGGCAGGTT+AGG 0.372839 4.4:-41931807 MS.gene021136:CDS
TGATCAGAAACAGGAGTATC+AGG 0.375983 4.4:+41933299 None:intergenic
ATTGCTATTGCTGTTGCTGC+TGG 0.378194 4.4:+41933361 None:intergenic
GGCCTTGAGGCACGCCTTGT+CGG 0.379088 4.4:-41932603 MS.gene021136:CDS
CTTCCCTATAGAAGGTTATA+TGG 0.379175 4.4:-41933603 MS.gene021136:CDS
ATTAGCATTCCTGTTGTCAT+TGG 0.384143 4.4:+41932704 None:intergenic
CGCCTTGTCGGCGATGGTAC+TGG 0.387590 4.4:-41932591 MS.gene021136:CDS
TAGTGATGGACTCAGAGATT+TGG 0.396758 4.4:-41933216 MS.gene021136:CDS
ATCCCGGTGATCTTCAGGTT+TGG 0.401423 4.4:+41932327 None:intergenic
AGGAAGCTATCATATTCATT+TGG 0.404865 4.4:+41933692 None:intergenic
ATTATCAGAGATGTTTGATA+AGG 0.416031 4.4:-41932910 MS.gene021136:CDS
AGAGAATCCGTTTGTGTTGA+AGG 0.418700 4.4:-41933036 MS.gene021136:CDS
TCCATTAATTCCGAATTCAA+TGG 0.422266 4.4:+41931757 None:intergenic
TACTACCACTTTGGTGAAGA+AGG 0.425321 4.4:-41933773 MS.gene021136:CDS
AACAGGAGTATCAGGTGAAA+GGG 0.430936 4.4:+41933307 None:intergenic
GAGGCCATATAACCTTCTAT+AGG 0.431388 4.4:+41933599 None:intergenic
CTGCCACTGTTTATATGTCT+CGG 0.432575 4.4:+41931813 None:intergenic
ATGAGCTACTGAAGCAGATT+AGG 0.435797 4.4:-41932683 MS.gene021136:CDS
GCCACCACCTTCCCTATAGA+AGG 0.439615 4.4:-41933611 MS.gene021136:CDS
AGGCAACATTCTTCACCCTC+TGG 0.447707 4.4:-41932663 MS.gene021136:CDS
CTGTATATGCCAATGACAAC+AGG 0.450042 4.4:-41932713 MS.gene021136:CDS
ACAAACGGTTACTACCACTT+TGG 0.452669 4.4:-41933782 MS.gene021136:CDS
AGAATGAAACAATAGATTTG+AGG 0.453249 4.4:-41932761 MS.gene021136:CDS
GGCAACATTCTTCACCCTCT+GGG 0.453926 4.4:-41932662 MS.gene021136:CDS
ATGGAAAGAATCGAAGAAAC+TGG 0.455010 4.4:-41932276 MS.gene021136:CDS
ATTTCTCAACCTCATTCTAC+TGG 0.458162 4.4:-41933242 MS.gene021136:CDS
TTTCTTGGTGCTGATCCACT+TGG 0.459039 4.4:-41933641 MS.gene021136:CDS
GTTTCTTCGATTCTTTCCAT+TGG 0.459734 4.4:+41932279 None:intergenic
TTCAAGAAAAGTTTAGAAGA+AGG 0.460061 4.4:-41933134 MS.gene021136:CDS
AGGCCATATAACCTTCTATA+GGG 0.460382 4.4:+41933600 None:intergenic
ATCCAATTTGGATATGGTTA+GGG 0.463279 4.4:-41933063 MS.gene021136:CDS
TTTCTACCCTTCAACACAAA+CGG 0.464697 4.4:+41933029 None:intergenic
GTGGTAACATTGCTGTAACT+TGG 0.472903 4.4:+41931723 None:intergenic
AGGTTATATGGCCTCGGCGA+CGG 0.474767 4.4:-41933591 MS.gene021136:CDS
GACAACAATTGGATGCTTCA+AGG 0.476004 4.4:-41931679 MS.gene021136:CDS
GGGATAGAGTTCCCTTTACC+TGG 0.477006 4.4:-41932309 MS.gene021136:CDS
GTAGAGATCCTCTTGGTGTT+CGG 0.479560 4.4:+41932546 None:intergenic
TGATTAACATGATCAGAAAC+AGG 0.483590 4.4:+41933290 None:intergenic
TGCCACTGTTTATATGTCTC+GGG 0.483709 4.4:+41931814 None:intergenic
CCGCAGCCTCAACTCTTGAC+TGG 0.485147 4.4:-41933095 MS.gene021136:CDS
GAGGAGTCACCTCCATCAAA+TGG 0.486751 4.4:-41932816 MS.gene021136:CDS
CAAGCTAGTGATTTCACAAA+CGG 0.489712 4.4:-41933797 MS.gene021136:CDS
GAATGAGGTTGAGAAATAGC+AGG 0.490215 4.4:+41933248 None:intergenic
TTGGCTCATTACTTTGCCAA+TGG 0.490767 4.4:-41932624 MS.gene021136:CDS
TTGGACGAAAACCAGGTAAA+GGG 0.491920 4.4:+41932298 None:intergenic
ACAATTGGATGCTTCAAGGT+TGG 0.492282 4.4:-41931675 MS.gene021136:CDS
GGGTGTTGATTAGGGGAAAG+AGG 0.492324 4.4:+41933413 None:intergenic
AAGAACAAGAAGAAGAAGCT+TGG 0.493244 4.4:-41932977 MS.gene021136:CDS
CTTTCCATTGGACGAAAACC+AGG 0.495527 4.4:+41932291 None:intergenic
CTGTCATTTGCAAGCTAAGT+GGG 0.500777 4.4:+41933474 None:intergenic
GAAAATGATTCCTTGCAGAA+TGG 0.503064 4.4:-41932852 MS.gene021136:CDS
TTGGCCACTGAAAACCATAT+AGG 0.503360 4.4:+41932388 None:intergenic
TGGGGATGGACCACAGAGGT+TGG 0.505585 4.4:-41932643 MS.gene021136:CDS
CCGGTGATCTTCAGGTTTGG+AGG 0.507786 4.4:+41932330 None:intergenic
TTCCCAATAACGCGATGGTA+TGG 0.510255 4.4:+41932200 None:intergenic
GCAGAATGGACAAGTGAAAA+CGG 0.510872 4.4:-41932838 MS.gene021136:CDS
AAGGCGTGCCTCAAGGCCAT+TGG 0.511609 4.4:+41932608 None:intergenic
TATAGAAGGTTATATGGCCT+CGG 0.515236 4.4:-41933597 MS.gene021136:CDS
CATTCTTCACCCTCTGGGGA+TGG 0.516297 4.4:-41932657 MS.gene021136:CDS
ATTCATTTGGATCAGCATCA+AGG 0.522321 4.4:+41933705 None:intergenic
TTGGGAAACAATTCGAGTTG+AGG 0.525214 4.4:-41932184 MS.gene021136:CDS
CGCCGACAAGGCGTGCCTCA+AGG 0.525318 4.4:+41932601 None:intergenic
CTAAACCGTGTAGCAAAGAA+TGG 0.525919 4.4:+41931988 None:intergenic
GGATTCTTCTATTACACAGA+TGG 0.528072 4.4:-41933195 MS.gene021136:CDS
GGTATGGCATTAAACTCAAA+GGG 0.528598 4.4:+41932216 None:intergenic
ATTGGACGAAAACCAGGTAA+AGG 0.530391 4.4:+41932297 None:intergenic
CATTAAACTCAAAGGGAACA+TGG 0.533197 4.4:+41932223 None:intergenic
AATGAGGTTGAGAAATAGCA+GGG 0.534181 4.4:+41933249 None:intergenic
TACCACGCGAAAACAAATAC+AGG 0.534979 4.4:-41931915 MS.gene021136:CDS
CGAGACATATAAACAGTGGC+AGG 0.537307 4.4:-41931812 MS.gene021136:CDS
CAGGTTAGGAATACAAGAGC+TGG 0.537591 4.4:-41931793 MS.gene021136:CDS
AAACAGGAGTATCAGGTGAA+AGG 0.542644 4.4:+41933306 None:intergenic
GTCAAGAGTTGAGGCTGCGG+AGG 0.544335 4.4:+41933098 None:intergenic
GAGAATCCGTTTGTGTTGAA+GGG 0.545933 4.4:-41933035 MS.gene021136:CDS
CTCCAGTACCATCGCCGACA+AGG 0.546196 4.4:+41932589 None:intergenic
TTAGATATTCATAACTCTGA+TGG 0.546938 4.4:-41933389 MS.gene021136:CDS
GTTCATAAACCAAATCCTGA+TGG 0.550468 4.4:-41933528 MS.gene021136:CDS
TCAGAGTTATGAATATCTAA+GGG 0.551258 4.4:+41933392 None:intergenic
TACCAAAATCAATGATATGG+AGG 0.553791 4.4:+41932412 None:intergenic
CTCCAAACCTGAAGATCACC+GGG 0.554977 4.4:-41932329 MS.gene021136:CDS
TGGTATGGCATTAAACTCAA+AGG 0.555962 4.4:+41932215 None:intergenic
AGGTAAAGGGAACTCTATCC+CGG 0.560031 4.4:+41932311 None:intergenic
CCTCCAAACCTGAAGATCAC+CGG 0.561178 4.4:-41932330 MS.gene021136:CDS
AAATCAGTGTCTTCGAGGGA+AGG 0.562271 4.4:+41933566 None:intergenic
GAGGCACGCCTTGTCGGCGA+TGG 0.563864 4.4:-41932597 MS.gene021136:CDS
TCTGTCATTTGCAAGCTAAG+TGG 0.564137 4.4:+41933473 None:intergenic
ATGAACGTTGTAGCTTGTGA+AGG 0.570356 4.4:-41931856 MS.gene021136:CDS
CATTCTACTGGTGTTAGTGA+TGG 0.570556 4.4:-41933230 MS.gene021136:CDS
CCAGTCAAGAGTTGAGGCTG+CGG 0.571987 4.4:+41933095 None:intergenic
ATTCGCCATTCTTTGCTACA+CGG 0.575217 4.4:-41931993 MS.gene021136:CDS
TGGATGCTTCAAGGTTGGAA+AGG 0.577566 4.4:-41931670 MS.gene021136:CDS
TCTTGGTGTTCGGAGAGGAA+TGG 0.578017 4.4:+41932556 None:intergenic
CTCAGCAGTAGAGATCCTCT+TGG 0.580706 4.4:+41932539 None:intergenic
GTGATGTGAGTTGATTATGC+TGG 0.583220 4.4:+41931616 None:intergenic
TACTCCCTTCTTCACCAAAG+TGG 0.586539 4.4:+41933768 None:intergenic
ACTACCACTTTGGTGAAGAA+GGG 0.587510 4.4:-41933772 MS.gene021136:CDS
CATATAACCTTCTATAGGGA+AGG 0.589911 4.4:+41933604 None:intergenic
CTTTGCTACACGGTTTAGGG+AGG 0.591419 4.4:-41931983 MS.gene021136:CDS
ATGGAAGCGCTTGCAATAGT+CGG 0.593097 4.4:+41932242 None:intergenic
TTACTTTGCCAATGGCCTTG+AGG 0.593344 4.4:-41932616 MS.gene021136:CDS
ATTGATTTGGATCAGCCTCA+AGG 0.595193 4.4:+41933672 None:intergenic
AAACAAATACAGGATGCTGA+TGG 0.598030 4.4:-41931905 MS.gene021136:CDS
CAATTTGGATATGGTTAGGG+AGG 0.598257 4.4:-41933060 MS.gene021136:CDS
CTCTGTGGTCCATCCCCAGA+GGG 0.598960 4.4:+41932648 None:intergenic
ACCTTCTATAGGGAAGGTGG+TGG 0.600256 4.4:+41933610 None:intergenic
TTTCACTTGTCCATTCTGCA+AGG 0.603209 4.4:+41932842 None:intergenic
ATCTAAGGGGTGTTGATTAG+GGG 0.605109 4.4:+41933406 None:intergenic
ACCAAAATCAATGATATGGA+GGG 0.610467 4.4:+41932413 None:intergenic
ATAACCTTCTATAGGGAAGG+TGG 0.616055 4.4:+41933607 None:intergenic
AAGCTAAGTGGGTCATAGAA+CGG 0.617850 4.4:+41933485 None:intergenic
ATACAGGATGCTGATGGAGA+GGG 0.620955 4.4:-41931899 MS.gene021136:CDS
ATTGTTTCCCAATAACGCGA+TGG 0.623674 4.4:+41932195 None:intergenic
GTCTTCCCTCCATTTGATGG+AGG 0.626646 4.4:+41932807 None:intergenic
GAGTCACCTCCATCAAATGG+AGG 0.628730 4.4:-41932813 MS.gene021136:CDS
TCACTAACACCAGTAGAATG+AGG 0.628786 4.4:+41933233 None:intergenic
TCAAAACCAGTCAAGAGTTG+AGG 0.630836 4.4:+41933089 None:intergenic
AATACAGGATGCTGATGGAG+AGG 0.637418 4.4:-41931900 MS.gene021136:CDS
ACAGGAGTATCAGGTGAAAG+GGG 0.642453 4.4:+41933308 None:intergenic
ATTCCTCTCCGAACACCAAG+AGG 0.643154 4.4:-41932554 MS.gene021136:CDS
AGTCACCTCCATCAAATGGA+GGG 0.643984 4.4:-41932812 MS.gene021136:CDS
CCTCTGTGGTCCATCCCCAG+AGG 0.644825 4.4:+41932647 None:intergenic
GCAACATTCTTCACCCTCTG+GGG 0.646104 4.4:-41932661 MS.gene021136:CDS
TTATGCTGGCACCCAACATG+TGG 0.647174 4.4:+41931630 None:intergenic
AAGGTTTAGAGAGAGTTGAG+AGG 0.649221 4.4:-41931837 MS.gene021136:CDS
AAGACTCGTCGTAAGAAACA+AGG 0.649427 4.4:-41932789 MS.gene021136:CDS
ATGAGGTTGAGAAATAGCAG+GGG 0.658762 4.4:+41933250 None:intergenic
GATCCTCTTGGTGTTCGGAG+AGG 0.659641 4.4:+41932551 None:intergenic
AGCTATTAGTGCTATTGACG+AGG 0.669842 4.4:-41932937 MS.gene021136:CDS
CAATAGCAATAGCAGCGTCG+AGG 0.672364 4.4:-41933348 MS.gene021136:CDS
CAGAGTTATGAATATCTAAG+GGG 0.672632 4.4:+41933393 None:intergenic
TGAATATGATAGCTTCCTTG+AGG 0.674292 4.4:-41933687 MS.gene021136:CDS
TTTGTGAAATCACTAGCTTG+AGG 0.676421 4.4:+41933800 None:intergenic
AGCAAACAGTCCTAGAAATG+CGG 0.677440 4.4:-41932082 MS.gene021136:CDS
AAGTAATGAGCCAACCTCTG+TGG 0.682694 4.4:+41932633 None:intergenic
GAGGGAAGGATCCGTCGCCG+AGG 0.685761 4.4:+41933580 None:intergenic
TCTTAAAATCAAGAGCAATG+AGG 0.688807 4.4:-41932160 MS.gene021136:CDS
GTGGTGGCTGCAAAACCAAG+TGG 0.689009 4.4:+41933626 None:intergenic
TGGTGAAGAAGGGAGTACAG+TGG 0.697331 4.4:-41933762 MS.gene021136:CDS
GGCCCGAGACATATAAACAG+TGG 0.698214 4.4:-41931816 MS.gene021136:CDS
CTGATGGAGAGGGAAAGTAT+CGG 0.714910 4.4:-41931889 MS.gene021136:CDS
GAATGGACAAGTGAAAACGG+AGG 0.763230 4.4:-41932835 MS.gene021136:CDS
CCTCTGGGGATGGACCACAG+AGG 0.797161 4.4:-41932647 MS.gene021136:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAAAAACTTTATAAGTAT+TGG + chr4.4:41933079-41933098 None:intergenic 15.0%
!! GATGAGATTTAAGAATTTAT+TGG - chr4.4:41933327-41933346 MS.gene021136:CDS 20.0%
!!! TTCTAAACTTTTCTTGAATA+GGG + chr4.4:41932309-41932328 None:intergenic 20.0%
! AATCAATATGATAGCTTTCT+TGG - chr4.4:41931789-41931808 MS.gene021136:CDS 25.0%
! AGAATGAAACAATAGATTTG+AGG - chr4.4:41932684-41932703 MS.gene021136:CDS 25.0%
! ATCAGAGTTATGAATATCTA+AGG + chr4.4:41932057-41932076 None:intergenic 25.0%
! ATTATCAGAGATGTTTGATA+AGG - chr4.4:41932535-41932554 MS.gene021136:CDS 25.0%
! TCAGAGTTATGAATATCTAA+GGG + chr4.4:41932056-41932075 None:intergenic 25.0%
! TTAGATATTCATAACTCTGA+TGG - chr4.4:41932056-41932075 MS.gene021136:CDS 25.0%
! TTCAAGAAAAGTTTAGAAGA+AGG - chr4.4:41932311-41932330 MS.gene021136:CDS 25.0%
! TTTGTTCAGTCAATTGTTAA+TGG - chr4.4:41933421-41933440 MS.gene021136:CDS 25.0%
!! AAGTATGCAAACTTTTTGAA+AGG + chr4.4:41932968-41932987 None:intergenic 25.0%
!! AAGTTTTTATCAGATAGAGA+AGG - chr4.4:41933088-41933107 MS.gene021136:CDS 25.0%
!! AGAAAGCTATCATATTGATT+TGG + chr4.4:41931789-41931808 None:intergenic 25.0%
!! CTTCTAAACTTTTCTTGAAT+AGG + chr4.4:41932310-41932329 None:intergenic 25.0%
!! GAATCATTTTCATTGCATAA+TGG + chr4.4:41932584-41932603 None:intergenic 25.0%
!! TTTTTGATGTAGACAACAAT+TGG - chr4.4:41933755-41933774 MS.gene021136:CDS 25.0%
!!! ATTGATTTTGGTATCCTATA+TGG - chr4.4:41933043-41933062 MS.gene021136:CDS 25.0%
ACCAAAATCAATGATATGGA+GGG + chr4.4:41933035-41933054 None:intergenic 30.0%
AGGAAGCTATCATATTCATT+TGG + chr4.4:41931756-41931775 None:intergenic 30.0%
ATCCAATTTGGATATGGTTA+GGG - chr4.4:41932382-41932401 MS.gene021136:CDS 30.0%
ATTGACTGAACAAAAATAGC+AGG + chr4.4:41933415-41933434 None:intergenic 30.0%
CAGAGTTATGAATATCTAAG+GGG + chr4.4:41932055-41932074 None:intergenic 30.0%
CATCAAAAACAAAGTCTTTG+TGG + chr4.4:41933744-41933763 None:intergenic 30.0%
GGATACCAAAATCAATGATA+TGG + chr4.4:41933039-41933058 None:intergenic 30.0%
TACCAAAATCAATGATATGG+AGG + chr4.4:41933036-41933055 None:intergenic 30.0%
TCCATTAATTCCGAATTCAA+TGG + chr4.4:41933691-41933710 None:intergenic 30.0%
TCCATTGAATTCGGAATTAA+TGG - chr4.4:41933687-41933706 MS.gene021136:CDS 30.0%
TCTTAAAATCAAGAGCAATG+AGG - chr4.4:41933285-41933304 MS.gene021136:CDS 30.0%
TGAATCATCCAATTTGGATA+TGG - chr4.4:41932376-41932395 MS.gene021136:CDS 30.0%
TGATTAACATGATCAGAAAC+AGG + chr4.4:41932158-41932177 None:intergenic 30.0%
TTTATATGCTTCCACATGTT+GGG - chr4.4:41933804-41933823 MS.gene021136:CDS 30.0%
! AGGAAGCTATCATATTGATT+TGG + chr4.4:41931723-41931742 None:intergenic 30.0%
! CGGAATTAATGGACAAATTT+AGG - chr4.4:41933698-41933717 MS.gene021136:CDS 30.0%
! TTTTATATGCTTCCACATGT+TGG - chr4.4:41933803-41933822 MS.gene021136:CDS 30.0%
! TTTTTATCAGATAGAGAAGG+TGG - chr4.4:41933091-41933110 MS.gene021136:CDS 30.0%
!!! TGGTTTTGAATCATCCAATT+TGG - chr4.4:41932370-41932389 MS.gene021136:CDS 30.0%
AAACAAATACAGGATGCTGA+TGG - chr4.4:41933540-41933559 MS.gene021136:CDS 35.0%
AAGAACAAGAAGAAGAAGCT+TGG - chr4.4:41932468-41932487 MS.gene021136:CDS 35.0%
AATGAGGTTGAGAAATAGCA+GGG + chr4.4:41932199-41932218 None:intergenic 35.0%
AGGCCATATAACCTTCTATA+GGG + chr4.4:41931848-41931867 None:intergenic 35.0%
ATGGAAAGAATCGAAGAAAC+TGG - chr4.4:41933169-41933188 MS.gene021136:CDS 35.0%
ATTAGCATTCCTGTTGTCAT+TGG + chr4.4:41932744-41932763 None:intergenic 35.0%
ATTCATTTGGATCAGCATCA+AGG + chr4.4:41931743-41931762 None:intergenic 35.0%
ATTCTTTGCTACACGGTTTA+GGG - chr4.4:41933459-41933478 MS.gene021136:CDS 35.0%
ATTTCTCAACCTCATTCTAC+TGG - chr4.4:41932203-41932222 MS.gene021136:CDS 35.0%
CAAACAGCTTCCATTGAATT+CGG - chr4.4:41933678-41933697 MS.gene021136:CDS 35.0%
CAAGCTAGTGATTTCACAAA+CGG - chr4.4:41931648-41931667 MS.gene021136:CDS 35.0%
CATATAACCTTCTATAGGGA+AGG + chr4.4:41931844-41931863 None:intergenic 35.0%
CATCCAATTTGGATATGGTT+AGG - chr4.4:41932381-41932400 MS.gene021136:CDS 35.0%
CATTAAACTCAAAGGGAACA+TGG + chr4.4:41933225-41933244 None:intergenic 35.0%
CTTCCCTATAGAAGGTTATA+TGG - chr4.4:41931842-41931861 MS.gene021136:CDS 35.0%
GAAAATGATTCCTTGCAGAA+TGG - chr4.4:41932593-41932612 MS.gene021136:CDS 35.0%
GGATTCTTCTATTACACAGA+TGG - chr4.4:41932250-41932269 MS.gene021136:CDS 35.0%
GGTATGGCATTAAACTCAAA+GGG + chr4.4:41933232-41933251 None:intergenic 35.0%
GTTCATAAACCAAATCCTGA+TGG - chr4.4:41931917-41931936 MS.gene021136:CDS 35.0%
GTTTCTTCGATTCTTTCCAT+TGG + chr4.4:41933169-41933188 None:intergenic 35.0%
TATAGAAGGTTATATGGCCT+CGG - chr4.4:41931848-41931867 MS.gene021136:CDS 35.0%
TGAAAAATCAGTGTCTTCGA+GGG + chr4.4:41931886-41931905 None:intergenic 35.0%
TGAATATGATAGCTTCCTTG+AGG - chr4.4:41931758-41931777 MS.gene021136:CDS 35.0%
TGGTATGGCATTAAACTCAA+AGG + chr4.4:41933233-41933252 None:intergenic 35.0%
TTTCTACCCTTCAACACAAA+CGG + chr4.4:41932419-41932438 None:intergenic 35.0%
! CATTTTCTTCCATCAGGATT+TGG + chr4.4:41931929-41931948 None:intergenic 35.0%
! GATAAGGTTTTGCTTGATGT+GGG - chr4.4:41932551-41932570 MS.gene021136:CDS 35.0%
! GATCAACATTTTCTTCCATC+AGG + chr4.4:41931935-41931954 None:intergenic 35.0%
! GTATCCTATATGGTTTTCAG+TGG - chr4.4:41933053-41933072 MS.gene021136:CDS 35.0%
! TGATAAGGTTTTGCTTGATG+TGG - chr4.4:41932550-41932569 MS.gene021136:CDS 35.0%
! TTTGTGAAATCACTAGCTTG+AGG + chr4.4:41931648-41931667 None:intergenic 35.0%
!! ATATCTAAGGGGTGTTGATT+AGG + chr4.4:41932044-41932063 None:intergenic 35.0%
!! TATCTAAGGGGTGTTGATTA+GGG + chr4.4:41932043-41932062 None:intergenic 35.0%
!!! ACCCTCCATATCATTGATTT+TGG - chr4.4:41933031-41933050 MS.gene021136:CDS 35.0%
!!! GACACTGATTTTTCAGATAC+TGG - chr4.4:41931891-41931910 MS.gene021136:CDS 35.0%
AAATGCAGAACCGCATTTCT+AGG + chr4.4:41933376-41933395 None:intergenic 40.0%
AAGACTCGTCGTAAGAAACA+AGG - chr4.4:41932656-41932675 MS.gene021136:CDS 40.0%
AAGCTAAGTGGGTCATAGAA+CGG + chr4.4:41931963-41931982 None:intergenic 40.0%
AAGGTTTAGAGAGAGTTGAG+AGG - chr4.4:41933608-41933627 MS.gene021136:CDS 40.0%
ACAAACGGTTACTACCACTT+TGG - chr4.4:41931663-41931682 MS.gene021136:CDS 40.0%
ACAATTGGATGCTTCAAGGT+TGG - chr4.4:41933770-41933789 MS.gene021136:CDS 40.0%
ACTACCACTTTGGTGAAGAA+GGG - chr4.4:41931673-41931692 MS.gene021136:CDS 40.0%
AGAGAATCCGTTTGTGTTGA+AGG - chr4.4:41932409-41932428 MS.gene021136:CDS 40.0%
AGCAAACAGTCCTAGAAATG+CGG - chr4.4:41933363-41933382 MS.gene021136:CDS 40.0%
ATAACCTTCTATAGGGAAGG+TGG + chr4.4:41931841-41931860 None:intergenic 40.0%
ATGAGCTACTGAAGCAGATT+AGG - chr4.4:41932762-41932781 MS.gene021136:CDS 40.0%
ATGAGGTTGAGAAATAGCAG+GGG + chr4.4:41932198-41932217 None:intergenic 40.0%
ATTCGCCATTCTTTGCTACA+CGG - chr4.4:41933452-41933471 MS.gene021136:CDS 40.0%
ATTGGACGAAAACCAGGTAA+AGG + chr4.4:41933151-41933170 None:intergenic 40.0%
ATTGTTTCCCAATAACGCGA+TGG + chr4.4:41933253-41933272 None:intergenic 40.0%
CAATTTGGATATGGTTAGGG+AGG - chr4.4:41932385-41932404 MS.gene021136:CDS 40.0%
CATATAAACAGTGGCAGGTT+AGG - chr4.4:41933638-41933657 MS.gene021136:CDS 40.0%
CATTCTTTGCTACACGGTTT+AGG - chr4.4:41933458-41933477 MS.gene021136:CDS 40.0%
CTAAACCGTGTAGCAAAGAA+TGG + chr4.4:41933460-41933479 None:intergenic 40.0%
CTCCCTAACCATATCCAAAT+TGG + chr4.4:41932387-41932406 None:intergenic 40.0%
CTGAAAAATCAGTGTCTTCG+AGG + chr4.4:41931887-41931906 None:intergenic 40.0%
CTGCCACTGTTTATATGTCT+CGG + chr4.4:41933635-41933654 None:intergenic 40.0%
CTGTATATGCCAATGACAAC+AGG - chr4.4:41932732-41932751 MS.gene021136:CDS 40.0%
GAATGAGGTTGAGAAATAGC+AGG + chr4.4:41932200-41932219 None:intergenic 40.0%
GACAACAATTGGATGCTTCA+AGG - chr4.4:41933766-41933785 MS.gene021136:CDS 40.0%
GAGAATCCGTTTGTGTTGAA+GGG - chr4.4:41932410-41932429 MS.gene021136:CDS 40.0%
GAGGCCATATAACCTTCTAT+AGG + chr4.4:41931849-41931868 None:intergenic 40.0%
GCAGAATGGACAAGTGAAAA+CGG - chr4.4:41932607-41932626 MS.gene021136:CDS 40.0%
GTGGTAACATTGCTGTAACT+TGG + chr4.4:41933725-41933744 None:intergenic 40.0%
TACCACGCGAAAACAAATAC+AGG - chr4.4:41933530-41933549 MS.gene021136:CDS 40.0%
TACTACCACTTTGGTGAAGA+AGG - chr4.4:41931672-41931691 MS.gene021136:CDS 40.0%
TCAAAACCAGTCAAGAGTTG+AGG + chr4.4:41932359-41932378 None:intergenic 40.0%
TCACTAACACCAGTAGAATG+AGG + chr4.4:41932215-41932234 None:intergenic 40.0%
TGCCACTGTTTATATGTCTC+GGG + chr4.4:41933634-41933653 None:intergenic 40.0%
TTGGACGAAAACCAGGTAAA+GGG + chr4.4:41933150-41933169 None:intergenic 40.0%
TTGGCCACTGAAAACCATAT+AGG + chr4.4:41933060-41933079 None:intergenic 40.0%
TTGGCTCATTACTTTGCCAA+TGG - chr4.4:41932821-41932840 MS.gene021136:CDS 40.0%
TTTCACTTGTCCATTCTGCA+AGG + chr4.4:41932606-41932625 None:intergenic 40.0%
! AGCTATTAGTGCTATTGACG+AGG - chr4.4:41932508-41932527 MS.gene021136:CDS 40.0%
! ATGAACGTTGTAGCTTGTGA+AGG - chr4.4:41933589-41933608 MS.gene021136:CDS 40.0%
! CATTCTACTGGTGTTAGTGA+TGG - chr4.4:41932215-41932234 MS.gene021136:CDS 40.0%
! CTGTCATTTGCAAGCTAAGT+GGG + chr4.4:41931974-41931993 None:intergenic 40.0%
! TCACTAGCTTGAGGAAAGTT+TGG + chr4.4:41931639-41931658 None:intergenic 40.0%
! TCTGTCATTTGCAAGCTAAG+TGG + chr4.4:41931975-41931994 None:intergenic 40.0%
!! AAACAGGAGTATCAGGTGAA+AGG + chr4.4:41932142-41932161 None:intergenic 40.0%
!! AACAGGAGTATCAGGTGAAA+GGG + chr4.4:41932141-41932160 None:intergenic 40.0%
!! ATCCTGTATTTGTTTTCGCG+TGG + chr4.4:41933535-41933554 None:intergenic 40.0%
!! ATCTAAGGGGTGTTGATTAG+GGG + chr4.4:41932042-41932061 None:intergenic 40.0%
!! ATTGATTTGGATCAGCCTCA+AGG + chr4.4:41931776-41931795 None:intergenic 40.0%
!! TAGTGATGGACTCAGAGATT+TGG - chr4.4:41932229-41932248 MS.gene021136:CDS 40.0%
!! TGATCAGAAACAGGAGTATC+AGG + chr4.4:41932149-41932168 None:intergenic 40.0%
!! TTGGGAAACAATTCGAGTTG+AGG - chr4.4:41933261-41933280 MS.gene021136:CDS 40.0%
!! TTTACCTGGTTTTCGTCCAA+TGG - chr4.4:41933150-41933169 MS.gene021136:CDS 40.0%
AAATCAGTGTCTTCGAGGGA+AGG + chr4.4:41931882-41931901 None:intergenic 45.0%
AAGTAATGAGCCAACCTCTG+TGG + chr4.4:41932815-41932834 None:intergenic 45.0%
AATACAGGATGCTGATGGAG+AGG - chr4.4:41933545-41933564 MS.gene021136:CDS 45.0%
AGGTAAAGGGAACTCTATCC+CGG + chr4.4:41933137-41933156 None:intergenic 45.0%
AGTCACCTCCATCAAATGGA+GGG - chr4.4:41932633-41932652 MS.gene021136:CDS 45.0%
ATACAGGATGCTGATGGAGA+GGG - chr4.4:41933546-41933565 MS.gene021136:CDS 45.0%
ATGCCATACCATCGCGTTAT+TGG - chr4.4:41933242-41933261 MS.gene021136:CDS 45.0%
CAGGTTAGGAATACAAGAGC+TGG - chr4.4:41933652-41933671 MS.gene021136:CDS 45.0%
CGAGACATATAAACAGTGGC+AGG - chr4.4:41933633-41933652 MS.gene021136:CDS 45.0%
CTTTCCATTGGACGAAAACC+AGG + chr4.4:41933157-41933176 None:intergenic 45.0%
GAATGGACAAGTGAAAACGG+AGG - chr4.4:41932610-41932629 MS.gene021136:CDS 45.0%
TACTCCCTTCTTCACCAAAG+TGG + chr4.4:41931680-41931699 None:intergenic 45.0%
TGCCATACCATCGCGTTATT+GGG - chr4.4:41933243-41933262 MS.gene021136:CDS 45.0%
TGGATGCTTCAAGGTTGGAA+AGG - chr4.4:41933775-41933794 MS.gene021136:CDS 45.0%
TTCCCAATAACGCGATGGTA+TGG + chr4.4:41933248-41933267 None:intergenic 45.0%
! ATTGCTATTGCTGTTGCTGC+TGG + chr4.4:41932087-41932106 None:intergenic 45.0%
! CTGATGGAGAGGGAAAGTAT+CGG - chr4.4:41933556-41933575 MS.gene021136:CDS 45.0%
! TTACTTTGCCAATGGCCTTG+AGG - chr4.4:41932829-41932848 MS.gene021136:CDS 45.0%
!! ACAGGAGTATCAGGTGAAAG+GGG + chr4.4:41932140-41932159 None:intergenic 45.0%
!! ATGGAAGCGCTTGCAATAGT+CGG + chr4.4:41933206-41933225 None:intergenic 45.0%
!! GTAGAGATCCTCTTGGTGTT+CGG + chr4.4:41932902-41932921 None:intergenic 45.0%
!! TTTCTTGGTGCTGATCCACT+TGG - chr4.4:41931804-41931823 MS.gene021136:CDS 45.0%
ACCTTCTATAGGGAAGGTGG+TGG + chr4.4:41931838-41931857 None:intergenic 50.0%
ACTCTATCCCGGTGATCTTC+AGG + chr4.4:41933126-41933145 None:intergenic 50.0%
AGGCAACATTCTTCACCCTC+TGG - chr4.4:41932782-41932801 MS.gene021136:CDS 50.0%
ATTCCTCTCCGAACACCAAG+AGG - chr4.4:41932891-41932910 MS.gene021136:CDS 50.0%
CAATAGCAATAGCAGCGTCG+AGG - chr4.4:41932097-41932116 MS.gene021136:CDS 50.0%
CCTCCAAACCTGAAGATCAC+CGG - chr4.4:41933115-41933134 MS.gene021136:CDS 50.0%
CGAGTCTTCCCTCCATTTGA+TGG + chr4.4:41932644-41932663 None:intergenic 50.0%
CTCAGCAGTAGAGATCCTCT+TGG + chr4.4:41932909-41932928 None:intergenic 50.0%
CTCCAAACCTGAAGATCACC+GGG - chr4.4:41933116-41933135 MS.gene021136:CDS 50.0%
CTTTGCTACACGGTTTAGGG+AGG - chr4.4:41933462-41933481 MS.gene021136:CDS 50.0%
GAGGAGTCACCTCCATCAAA+TGG - chr4.4:41932629-41932648 MS.gene021136:CDS 50.0%
GAGTCACCTCCATCAAATGG+AGG - chr4.4:41932632-41932651 MS.gene021136:CDS 50.0%
GCAACATTCTTCACCCTCTG+GGG - chr4.4:41932784-41932803 MS.gene021136:CDS 50.0%
GGCAACATTCTTCACCCTCT+GGG - chr4.4:41932783-41932802 MS.gene021136:CDS 50.0%
GGCCCGAGACATATAAACAG+TGG - chr4.4:41933629-41933648 MS.gene021136:CDS 50.0%
GGGATAGAGTTCCCTTTACC+TGG - chr4.4:41933136-41933155 MS.gene021136:CDS 50.0%
GTCTTCCCTCCATTTGATGG+AGG + chr4.4:41932641-41932660 None:intergenic 50.0%
TTATGCTGGCACCCAACATG+TGG + chr4.4:41933818-41933837 None:intergenic 50.0%
! ATCCCGGTGATCTTCAGGTT+TGG + chr4.4:41933121-41933140 None:intergenic 50.0%
! TGGTGAAGAAGGGAGTACAG+TGG - chr4.4:41931683-41931702 MS.gene021136:CDS 50.0%
!! GGGTGTTGATTAGGGGAAAG+AGG + chr4.4:41932035-41932054 None:intergenic 50.0%
!! TCTTGGTGTTCGGAGAGGAA+TGG + chr4.4:41932892-41932911 None:intergenic 50.0%
AGGTTATATGGCCTCGGCGA+CGG - chr4.4:41931854-41931873 MS.gene021136:CDS 55.0%
CCAGTCAAGAGTTGAGGCTG+CGG + chr4.4:41932353-41932372 None:intergenic 55.0%
GCCACCACCTTCCCTATAGA+AGG - chr4.4:41931834-41931853 MS.gene021136:CDS 55.0%
GTGGTGGCTGCAAAACCAAG+TGG + chr4.4:41931822-41931841 None:intergenic 55.0%
! CATTCTTCACCCTCTGGGGA+TGG - chr4.4:41932788-41932807 MS.gene021136:CDS 55.0%
! CCGGTGATCTTCAGGTTTGG+AGG + chr4.4:41933118-41933137 None:intergenic 55.0%
!! GATCCTCTTGGTGTTCGGAG+AGG + chr4.4:41932897-41932916 None:intergenic 55.0%
AAGGCGTGCCTCAAGGCCAT+TGG + chr4.4:41932840-41932859 None:intergenic 60.0%
CCGCAGCCTCAACTCTTGAC+TGG - chr4.4:41932350-41932369 MS.gene021136:CDS 60.0%
CTCCAGTACCATCGCCGACA+AGG + chr4.4:41932859-41932878 None:intergenic 60.0%
CTCTGTGGTCCATCCCCAGA+GGG + chr4.4:41932800-41932819 None:intergenic 60.0%
GTCAAGAGTTGAGGCTGCGG+AGG + chr4.4:41932350-41932369 None:intergenic 60.0%
TGGGGATGGACCACAGAGGT+TGG - chr4.4:41932802-41932821 MS.gene021136:CDS 60.0%
CCTCTGTGGTCCATCCCCAG+AGG + chr4.4:41932801-41932820 None:intergenic 65.0%
CGCCTTGTCGGCGATGGTAC+TGG - chr4.4:41932854-41932873 MS.gene021136:CDS 65.0%
GGCCTTGAGGCACGCCTTGT+CGG - chr4.4:41932842-41932861 MS.gene021136:CDS 65.0%
! CCTCTGGGGATGGACCACAG+AGG - chr4.4:41932798-41932817 MS.gene021136:CDS 65.0%
CGCCGACAAGGCGTGCCTCA+AGG + chr4.4:41932847-41932866 None:intergenic 70.0%
GAGGCACGCCTTGTCGGCGA+TGG - chr4.4:41932848-41932867 MS.gene021136:CDS 70.0%
GAGGGAAGGATCCGTCGCCG+AGG + chr4.4:41931868-41931887 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 41931630 41933837 41931630 ID=MS.gene021136
chr4.4 mRNA 41931630 41933837 41931630 ID=MS.gene021136.t1;Parent=MS.gene021136
chr4.4 exon 41931630 41933837 41931630 ID=MS.gene021136.t1.exon1;Parent=MS.gene021136.t1
chr4.4 CDS 41931630 41933837 41931630 ID=cds.MS.gene021136.t1;Parent=MS.gene021136.t1
Gene Sequence

>MS.gene021136

ATGGATCCAAACTTTCCTCAAGCTAGTGATTTCACAAACGGTTACTACCACTTTGGTGAAGAAGGGAGTACAGTGGTTTCTGAGTTTGATCCAAATCAATATGATAGCTTCCTTGATGCTGATCCAAATGAATATGATAGCTTCCTTGAGGCTGATCCAAATCAATATGATAGCTTTCTTGGTGCTGATCCACTTGGTTTTGCAGCCACCACCTTCCCTATAGAAGGTTATATGGCCTCGGCGACGGATCCTTCCCTCGAAGACACTGATTTTTCAGATACTGGTAAGTTCATAAACCAAATCCTGATGGAAGAAAATGTTGATCAGAGACCGTTCTATGACCCACTTAGCTTGCAAATGACAGAAAAATCATTCTATGATGCTCTCTTACACACAAATAAACCTCTTTCCCCTAATCAACACCCCTTAGATATTCATAACTCTGATGGCACAACCAGCAGCAACAGCAATAGCAATAGCAGCGTCGAGGATTCTCGAGAGTTAAAACCCCTTTCACCTGATACTCCTGTTTCTGATCATGTTAATCATGTTTTTCAGTTTAATTCCCCTGCTATTTCTCAACCTCATTCTACTGGTGTTAGTGATGGACTCAGAGATTTGGATTCTTCTATTACACAGATGGCACAAAACATTTTCAGTGATGTAGATTCTGTTTCCCTATTCAAGAAAAGTTTAGAAGAAGGCAACAAGTTTCTTCCTCCGCAGCCTCAACTCTTGACTGGTTTTGAATCATCCAATTTGGATATGGTTAGGGAGGAAGAGAATCCGTTTGTGTTGAAGGGTAGAAAGAATCACAAGCGCGAAGAAAGTTTTAGTAAAGAACAAGAAGAAGAAGCTTGGAGAAGTAGCAAGCAATCAGCTATTAGTGCTATTGACGAGGATGAATTATCAGAGATGTTTGATAAGGTTTTGCTTGATGTGGGACATTTGCCATTATGCAATGAAAATGATTCCTTGCAGAATGGACAAGTGAAAACGGAGGAGTCACCTCCATCAAATGGAGGGAAGACTCGTCGTAAGAAACAAGGCAAGAAGAATGAAACAATAGATTTGAGGAATCTTCTGCTTATGTGTTCACAATCTGTATATGCCAATGACAACAGGAATGCTAATGAGCTACTGAAGCAGATTAGGCAACATTCTTCACCCTCTGGGGATGGACCACAGAGGTTGGCTCATTACTTTGCCAATGGCCTTGAGGCACGCCTTGTCGGCGATGGTACTGGAGCACAAACATTCCATTCCTCTCCGAACACCAAGAGGATCTCTACTGCTGAGTTTTTGAAAGCATATCAAGTTCATCTATCTACTAGTCCTTTCAAAAAGTTTGCATACTTCTTTGCAAATAAGATGATTATGAAAGCTTCTGCAAATGCTGAAACCCTCCATATCATTGATTTTGGTATCCTATATGGTTTTCAGTGGCCAATACTTATAAAGTTTTTATCAGATAGAGAAGGTGGACCTCCAAACCTGAAGATCACCGGGATAGAGTTCCCTTTACCTGGTTTTCGTCCAATGGAAAGAATCGAAGAAACTGGTCGTCGTCTTGCCGACTATTGCAAGCGCTTCCATGTTCCCTTTGAGTTTAATGCCATACCATCGCGTTATTGGGAAACAATTCGAGTTGAGGATCTTAAAATCAAGAGCAATGAGGTTGTTGTTGTGAACTCTTTGATGAGATTTAAGAATTTATTGGATGAAAGTATTGAAGCAAACAGTCCTAGAAATGCGGTTCTGCATTTAATCAGAAAGATAAATCCTGCTATTTTTGTTCAGTCAATTGTTAATGGATCATATAATTCGCCATTCTTTGCTACACGGTTTAGGGAGGCGCTGTTTCATTTTTCTGCTCTTTATGATATGTTTGACACTGTCATACCACGCGAAAACAAATACAGGATGCTGATGGAGAGGGAAAGTATCGGTAGAGAAGCTATGAACGTTGTAGCTTGTGAAGGTTTAGAGAGAGTTGAGAGGCCCGAGACATATAAACAGTGGCAGGTTAGGAATACAAGAGCTGGTTTCAAACAGCTTCCATTGAATTCGGAATTAATGGACAAATTTAGGACCAAGTTACAGCAATGTTACCACAAAGACTTTGTTTTTGATGTAGACAACAATTGGATGCTTCAAGGTTGGAAAGGACGCATTTTATATGCTTCCACATGTTGGGTGCCAGCATAA
Protein sequence

>MS.gene021136.t1

MDPNFPQASDFTNGYYHFGEEGSTVVSEFDPNQYDSFLDADPNEYDSFLEADPNQYDSFLGADPLGFAATTFPIEGYMASATDPSLEDTDFSDTGKFINQILMEENVDQRPFYDPLSLQMTEKSFYDALLHTNKPLSPNQHPLDIHNSDGTTSSNSNSNSSVEDSRELKPLSPDTPVSDHVNHVFQFNSPAISQPHSTGVSDGLRDLDSSITQMAQNIFSDVDSVSLFKKSLEEGNKFLPPQPQLLTGFESSNLDMVREEENPFVLKGRKNHKREESFSKEQEEEAWRSSKQSAISAIDEDELSEMFDKVLLDVGHLPLCNENDSLQNGQVKTEESPPSNGGKTRRKKQGKKNETIDLRNLLLMCSQSVYANDNRNANELLKQIRQHSSPSGDGPQRLAHYFANGLEARLVGDGTGAQTFHSSPNTKRISTAEFLKAYQVHLSTSPFKKFAYFFANKMIMKASANAETLHIIDFGILYGFQWPILIKFLSDREGGPPNLKITGIEFPLPGFRPMERIEETGRRLADYCKRFHVPFEFNAIPSRYWETIRVEDLKIKSNEVVVVNSLMRFKNLLDESIEANSPRNAVLHLIRKINPAIFVQSIVNGSYNSPFFATRFREALFHFSALYDMFDTVIPRENKYRMLMERESIGREAMNVVACEGLERVERPETYKQWQVRNTRAGFKQLPLNSELMDKFRTKLQQCYHKDFVFDVDNNWMLQGWKGRILYASTCWVPA