AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021138

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021138.t1 MTR_4g064200 96.189 656 17 2 1 652 1 652 0.0 1298
MS.gene021138.t1 MTR_2g097463 59.455 624 203 12 66 652 47 657 0.0 719
MS.gene021138.t1 MTR_2g097467 59.259 621 210 11 65 652 46 656 0.0 711
MS.gene021138.t1 MTR_2g097473 54.294 687 248 13 1 652 1 656 0.0 698
MS.gene021138.t1 MTR_7g062120 43.909 747 308 17 8 652 5 742 0.0 572
MS.gene021138.t1 MTR_4g064160 56.303 476 185 9 187 652 224 686 2.94e-180 528
MS.gene021138.t1 MTR_4g064120 46.573 642 274 17 53 652 14 628 4.51e-178 520
MS.gene021138.t1 MTR_4g064150 46.955 624 269 15 40 652 163 735 1.79e-174 514
MS.gene021138.t1 MTR_4g064180 45.794 642 279 16 53 652 14 628 5.44e-174 509
MS.gene021138.t1 MTR_2g097410 55.076 463 183 9 202 652 294 743 1.95e-173 512
MS.gene021138.t1 MTR_2g097390 40.865 624 318 15 60 652 40 643 5.39e-148 444
MS.gene021138.t1 MTR_2g097310 49.091 440 203 6 218 652 217 640 2.61e-147 442
MS.gene021138.t1 MTR_2g097350 47.921 457 215 8 203 652 202 642 2.86e-145 436
MS.gene021138.t1 MTR_2g097380 45.517 435 183 5 218 652 183 563 1.12e-129 394
MS.gene021138.t1 MTR_5g097480 33.245 376 246 4 277 649 170 543 2.32e-64 222
MS.gene021138.t1 MTR_2g026250 31.387 411 272 6 243 649 191 595 3.11e-63 220
MS.gene021138.t1 MTR_1g029420 33.604 369 240 4 284 649 225 591 4.27e-61 214
MS.gene021138.t1 MTR_2g082090 32.708 373 246 4 280 649 208 578 9.75e-61 213
MS.gene021138.t1 MTR_3g089055 31.436 369 248 4 284 649 203 569 3.34e-57 203
MS.gene021138.t1 MTR_3g089055 31.436 369 248 4 284 649 198 564 3.75e-57 203
MS.gene021138.t1 MTR_4g133660 32.626 377 246 6 279 650 170 543 5.40e-57 202
MS.gene021138.t1 MTR_4g104020 32.642 386 231 9 281 649 146 519 7.57e-57 201
MS.gene021138.t1 MTR_3g056110 31.510 384 254 7 271 649 162 541 3.31e-56 200
MS.gene021138.t1 MTR_5g094450 30.769 377 252 5 280 649 157 531 9.23e-56 198
MS.gene021138.t1 MTR_7g027190 31.202 391 236 6 279 649 295 672 3.82e-55 199
MS.gene021138.t1 MTR_7g074650 31.436 369 233 8 287 651 441 793 3.82e-48 181
MS.gene021138.t1 MTR_8g442410 29.974 387 249 6 279 652 150 527 4.84e-48 177
MS.gene021138.t1 MTR_3g065980 30.711 394 233 10 277 649 165 539 3.00e-47 175
MS.gene021138.t1 MTR_4g076140 27.378 431 244 8 281 649 45 468 3.79e-45 167
MS.gene021138.t1 MTR_2g034260 30.179 391 241 12 279 649 205 583 6.43e-42 160
MS.gene021138.t1 MTR_3g022830 27.990 418 240 8 285 649 26 435 2.89e-41 156
MS.gene021138.t1 MTR_4g102790 25.455 385 265 8 280 651 288 663 6.00e-40 156
MS.gene021138.t1 MTR_1g096030 30.273 403 236 13 276 649 72 458 9.56e-40 152
MS.gene021138.t1 MTR_4g097080 27.778 378 257 8 284 651 133 504 1.74e-39 152
MS.gene021138.t1 MTR_2g034250 28.941 387 252 8 279 649 205 584 2.00e-39 153
MS.gene021138.t1 MTR_2g089100 28.395 405 243 10 284 652 52 445 1.84e-36 142
MS.gene021138.t1 MTR_4g077760 27.807 374 248 9 285 649 194 554 1.39e-35 142
MS.gene021138.t1 MTR_5g015490 27.720 386 253 9 284 651 114 491 1.50e-35 140
MS.gene021138.t1 MTR_2g034280 26.753 385 260 9 279 649 198 574 1.16e-34 139
MS.gene021138.t1 MTR_4g076020 28.533 375 229 10 287 652 87 431 1.23e-34 137
MS.gene021138.t1 MTR_1g069725 27.857 420 263 15 243 650 72 463 3.96e-34 136
MS.gene021138.t1 MTR_7g109580 27.273 385 251 10 279 649 184 553 1.29e-33 136
MS.gene021138.t1 MTR_7g057230 30.000 290 189 6 366 649 375 656 1.05e-31 131
MS.gene021138.t1 MTR_3g072710 28.193 415 238 18 270 650 112 500 2.66e-30 125
MS.gene021138.t1 MTR_1g086970 24.566 403 268 11 268 649 90 477 7.99e-30 124
MS.gene021138.t1 MTR_5g058860 27.033 418 267 15 258 650 93 497 2.71e-28 119
MS.gene021138.t1 MTR_6g047750 26.649 379 251 10 285 649 296 661 4.05e-28 120
MS.gene021138.t1 MTR_5g009080 30.645 248 153 5 252 492 30 265 6.66e-28 118
MS.gene021138.t1 MTR_5g009080 30.000 150 104 1 500 649 329 477 1.03e-16 84.0
MS.gene021138.t1 MTR_8g093070 27.229 415 267 16 252 652 105 498 8.62e-27 115
MS.gene021138.t1 MTR_2g099110 26.667 390 253 11 284 651 74 452 9.99e-26 111
MS.gene021138.t1 MTR_4g095500 25.180 417 259 11 284 652 59 470 1.57e-24 107
MS.gene021138.t1 MTR_5g015950 23.923 418 265 12 284 652 35 448 1.74e-24 107
MS.gene021138.t1 MTR_3g053270 23.923 418 265 12 284 652 35 448 1.74e-24 107
MS.gene021138.t1 MTR_0092s0100 24.051 395 281 8 261 649 346 727 5.46e-19 92.0
MS.gene021138.t1 MTR_0092s0100 24.051 395 281 8 261 649 349 730 5.67e-19 91.7
MS.gene021138.t1 MTR_4g026485 26.633 199 138 3 261 457 248 440 5.31e-16 82.0
MS.gene021138.t1 MTR_8g020840 22.717 427 272 17 261 649 141 547 6.17e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021138.t1 AT2G29060 44.131 673 320 19 11 651 8 656 3.07e-179 524
MS.gene021138.t1 AT1G07530 55.973 452 190 6 208 652 318 767 1.27e-177 524
MS.gene021138.t1 AT5G59450 44.640 625 273 15 39 652 38 600 3.88e-173 507
MS.gene021138.t1 AT3G46600 46.755 601 248 10 59 652 46 581 2.06e-171 501
MS.gene021138.t1 AT2G29065 44.410 653 296 16 35 652 10 630 1.23e-167 494
MS.gene021138.t1 AT2G29060 52.298 457 207 8 203 651 240 693 3.73e-162 482
MS.gene021138.t1 AT2G29060 39.216 102 51 3 11 112 8 98 1.56e-11 68.2
MS.gene021138.t1 AT3G46600 52.980 453 189 5 205 652 116 549 1.43e-160 473
MS.gene021138.t1 AT2G37650 51.844 461 205 6 205 651 257 714 1.72e-159 476
MS.gene021138.t1 AT2G37650 51.844 461 205 6 205 651 245 702 1.83e-159 475
MS.gene021138.t1 AT3G46600 58.221 371 152 2 284 652 4 373 1.11e-155 454
MS.gene021138.t1 AT1G07520 51.648 455 195 10 208 652 92 531 2.68e-153 453
MS.gene021138.t1 AT1G07520 51.648 455 195 10 208 652 92 531 2.68e-153 453
MS.gene021138.t1 AT1G07520 51.648 455 195 10 208 652 256 695 1.40e-151 454
MS.gene021138.t1 AT1G07520 51.648 455 195 10 208 652 257 696 1.46e-151 454
MS.gene021138.t1 AT1G50600 34.234 444 269 9 213 649 98 525 1.70e-67 230
MS.gene021138.t1 AT1G50600 34.234 444 269 9 213 649 169 596 8.58e-67 230
MS.gene021138.t1 AT1G50600 34.234 444 269 9 213 649 173 600 1.54e-66 229
MS.gene021138.t1 AT5G48150 31.072 457 284 8 210 649 47 489 9.72e-63 216
MS.gene021138.t1 AT5G48150 31.072 457 284 8 210 649 47 489 9.72e-63 216
MS.gene021138.t1 AT5G48150 31.072 457 284 8 210 649 47 489 9.72e-63 216
MS.gene021138.t1 AT5G48150 31.072 457 284 8 210 649 47 489 9.72e-63 216
MS.gene021138.t1 AT4G17230 33.158 380 249 4 275 651 149 526 3.06e-57 202
MS.gene021138.t1 AT4G17230 33.158 380 249 4 275 651 149 526 3.06e-57 202
MS.gene021138.t1 AT2G04890 31.903 373 242 5 280 649 49 412 4.96e-57 199
MS.gene021138.t1 AT1G21450 31.806 371 245 6 284 649 225 592 1.85e-54 196
MS.gene021138.t1 AT3G54220 32.979 376 221 12 287 651 296 651 2.83e-49 182
MS.gene021138.t1 AT3G03450 31.620 389 230 12 277 649 176 544 1.06e-47 176
MS.gene021138.t1 AT1G66350 30.561 481 268 17 198 649 62 505 2.09e-47 175
MS.gene021138.t1 AT5G17490 30.233 387 232 11 277 649 153 515 1.20e-45 170
MS.gene021138.t1 AT1G14920 31.217 378 237 9 279 649 167 528 1.68e-45 170
MS.gene021138.t1 AT2G01570 31.135 379 236 10 279 649 219 580 4.26e-45 170
MS.gene021138.t1 AT5G66770 31.608 367 239 9 288 649 224 583 1.14e-43 166
MS.gene021138.t1 AT3G50650 30.618 356 232 7 302 649 193 541 4.91e-41 157
MS.gene021138.t1 AT4G37650 27.456 397 249 11 283 651 145 530 3.40e-39 152
MS.gene021138.t1 AT5G52510 27.895 380 246 10 287 649 271 639 2.68e-35 142
MS.gene021138.t1 AT5G41920 27.083 384 251 9 276 649 35 399 3.16e-35 138
MS.gene021138.t1 AT1G63100 27.336 428 278 12 245 650 238 654 5.17e-34 138
MS.gene021138.t1 AT1G63100 27.336 428 278 12 245 650 213 629 5.55e-34 138
MS.gene021138.t1 AT1G55580 26.923 390 214 11 313 649 73 444 5.79e-31 126
MS.gene021138.t1 AT3G49950 27.990 393 245 10 284 651 30 409 9.38e-31 125
MS.gene021138.t1 AT4G08250 30.194 361 226 14 303 649 128 476 1.09e-30 126
MS.gene021138.t1 AT1G50420 30.088 226 143 4 281 497 54 273 1.34e-28 120
MS.gene021138.t1 AT1G50420 30.667 150 103 1 500 649 330 478 8.24e-17 84.3
MS.gene021138.t1 AT3G13840 25.587 426 258 20 251 649 116 509 3.08e-19 92.0
MS.gene021138.t1 AT2G45160 22.506 391 253 14 285 649 272 638 2.80e-12 70.5
MS.gene021138.t1 AT3G60630 28.502 207 113 6 352 536 327 520 4.37e-11 66.6

Find 130 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATTCTGTTCCTTCTTC+TGG 0.154876 4.4:-41911942 MS.gene021138:CDS
TTCACTCTTTCCACCAGTTC+TGG 0.213949 4.4:+41910268 None:intergenic
TTCTGCTAGCTGTTTCTTGC+TGG 0.218999 4.4:-41910162 MS.gene021138:CDS
AATATTCAAACCCTATTCTT+AGG 0.247868 4.4:-41911884 MS.gene021138:CDS
TAAGTACTACGGTAGATTTA+TGG 0.267085 4.4:-41911263 MS.gene021138:CDS
TCGTCGCAATCACAGATTTC+TGG 0.282921 4.4:-41911348 MS.gene021138:CDS
GTATATTAGTGATATTCTTA+TGG 0.285545 4.4:-41911862 MS.gene021138:CDS
TCAAAGGATGTCGAATTTCT+TGG 0.296573 4.4:-41910995 MS.gene021138:CDS
TCTTATGGATGAAGAAGATT+TGG 0.306482 4.4:-41911847 MS.gene021138:CDS
ATCTTCCTCAGCCAGGATTT+AGG 0.323032 4.4:-41910822 MS.gene021138:CDS
CCACCAAGGCTTCGTATTAC+AGG 0.346264 4.4:-41910850 MS.gene021138:CDS
ACACCATGTATGAAAATGTT+TGG 0.351597 4.4:+41910556 None:intergenic
CTCAGAAGATTCAACGTTTG+AGG 0.353024 4.4:+41911914 None:intergenic
ACAATTGCCTTGCTCAGAAA+TGG 0.366886 4.4:-41910726 MS.gene021138:CDS
TCACTAATATACCTAAGAAT+AGG 0.370302 4.4:+41911873 None:intergenic
TCTGGTTCAGGAAGATCTAA+TGG 0.377826 4.4:-41911330 MS.gene021138:CDS
GTCTCTTCAACCCTTTCTGC+AGG 0.380596 4.4:+41910799 None:intergenic
CTTACAAGCAGTGGCAGGTT+AGG 0.393985 4.4:-41910327 MS.gene021138:CDS
GGATGGGAAGTGGGTTCTGC+AGG 0.394105 4.4:-41910200 MS.gene021138:CDS
CAATCACAGATTTCTGGTTC+AGG 0.395582 4.4:-41911342 MS.gene021138:CDS
TCTGTTAACGGATGCATGTT+TGG 0.401684 4.4:-41912047 MS.gene021138:CDS
GATGTTCTGGGATCCGGAAC+TGG 0.403473 4.4:-41911762 MS.gene021138:CDS
TATCAAACAATGATGAGAAA+TGG 0.404180 4.4:+41910476 None:intergenic
TCTGCTAGCTGTTTCTTGCT+GGG 0.404236 4.4:-41910161 MS.gene021138:CDS
TGATGATGTTCTTCTTTGTA+AGG 0.409875 4.4:-41911409 MS.gene021138:CDS
ATGATTGAGAAAGGGTTATT+CGG 0.411272 4.4:-41910409 MS.gene021138:CDS
ACTTCCATTGGCTCCAGAAC+TGG 0.415867 4.4:-41910281 MS.gene021138:CDS
TTCATACATGGTGTAGTGAA+TGG 0.416386 4.4:-41910547 MS.gene021138:CDS
AAATTCGACATCCTTTGAAA+TGG 0.417376 4.4:+41911000 None:intergenic
CACTAATATACCTAAGAATA+GGG 0.422212 4.4:+41911874 None:intergenic
AGTGAAAGAAATGGTGAAGA+AGG 0.423556 4.4:-41910251 MS.gene021138:CDS
ATGATATACTGAAGCAGATT+AGG 0.428145 4.4:-41911185 MS.gene021138:CDS
GTGAAGTACTGCAAGAGGTT+TGG 0.428838 4.4:-41910763 MS.gene021138:CDS
TGCCACTGCTTGTAAGTCTC+TGG 0.433473 4.4:+41910334 None:intergenic
AGCCTCCACATTATTGACTT+TGG 0.433913 4.4:-41910934 MS.gene021138:CDS
CGTTGTTGATTGCGATTCTC+CGG 0.436900 4.4:-41911685 MS.gene021138:CDS
TCATCAACTACAAAATCTTT+CGG 0.438965 4.4:+41910223 None:intergenic
GGTTATCTGATGAATGATAA+TGG 0.445118 4.4:-41911516 MS.gene021138:CDS
ACTAGAACTAGTTCCAGTTC+CGG 0.458242 4.4:+41911749 None:intergenic
GCAAGGCAATTGTACTCAAA+TGG 0.459316 4.4:+41910736 None:intergenic
TGATAAAATTCCTCATGATC+CGG 0.461532 4.4:-41911640 MS.gene021138:CDS
TCTATATAATTTGATTAAGC+AGG 0.461754 4.4:+41910136 None:intergenic
TTTGAGGTTATTCCAGAAGA+AGG 0.466979 4.4:+41911930 None:intergenic
AAACCTCGCTGATCTCTAAC+CGG 0.468294 4.4:+41911621 None:intergenic
TTAGCTCATTACTTTGCTGA+TGG 0.473385 4.4:-41911126 MS.gene021138:CDS
AGGATTTAGGCCTGCAGAAA+GGG 0.473613 4.4:-41910809 MS.gene021138:CDS
CTGATGAATGATAATGGTAT+TGG 0.482313 4.4:-41911510 MS.gene021138:CDS
TGGGTTCTGCAGGGTTGGAA+AGG 0.485460 4.4:-41910190 MS.gene021138:CDS
GGAAGTGGGTTCTGCAGGGT+TGG 0.485717 4.4:-41910195 MS.gene021138:CDS
CGCTGAAGTCCATCGCCACT+TGG 0.487409 4.4:+41911150 None:intergenic
AAGACATCGCGATCGAAGAA+AGG 0.489585 4.4:-41911306 MS.gene021138:CDS
CAATTGCCTTGCTCAGAAAT+GGG 0.493446 4.4:-41910725 MS.gene021138:CDS
ATGCTGTGTTGAGGTTGATC+AGG 0.494919 4.4:-41910588 MS.gene021138:CDS
GGGTTCTGCAGGGTTGGAAA+GGG 0.502336 4.4:-41910189 MS.gene021138:CDS
CAGGATTTAGGCCTGCAGAA+AGG 0.503621 4.4:-41910810 MS.gene021138:CDS
ATCGAAGAAAGGTTCCAGTA+AGG 0.504093 4.4:-41911295 MS.gene021138:CDS
AAACAATCTTGCAACATACA+AGG 0.512556 4.4:+41911816 None:intergenic
CTTTCTGCAGGCCTAAATCC+TGG 0.515059 4.4:+41910811 None:intergenic
TATAGGCTGATGATTGAGAA+AGG 0.518213 4.4:-41910418 MS.gene021138:CDS
CCTGTAATACGAAGCCTTGG+TGG 0.518595 4.4:+41910850 None:intergenic
GAAGAATGTTTCTGATGAAA+CGG 0.519403 4.4:-41910632 MS.gene021138:CDS
GTTTGATATGCTTGAAGCTA+CGG 0.522691 4.4:-41910461 MS.gene021138:CDS
CATTCTTCGCCAAGTGGCGA+TGG 0.522850 4.4:-41911159 MS.gene021138:CDS
TGAGAAAGGGTTATTCGGAA+GGG 0.524037 4.4:-41910404 MS.gene021138:CDS
GGAATTGATCTTCCTCAGCC+AGG 0.529102 4.4:-41910829 MS.gene021138:CDS
CAACAGAACTATTCTGAAGC+TGG 0.530899 4.4:-41910971 MS.gene021138:CDS
AAGGTTCAAACAACTTCCAT+TGG 0.531648 4.4:-41910293 MS.gene021138:CDS
TCCACCAGTTCTGGAGCCAA+TGG 0.532207 4.4:+41910277 None:intergenic
AAGGAGCAGAGAGGGTTGAA+AGG 0.532815 4.4:-41910357 MS.gene021138:CDS
TCGAAGAAAGGTTCCAGTAA+GGG 0.534571 4.4:-41911294 MS.gene021138:CDS
GCAGAAAGGGTTGAAGAGAC+AGG 0.536681 4.4:-41910796 MS.gene021138:CDS
GAAGGAACAGAATCACTTGA+AGG 0.539537 4.4:+41911948 None:intergenic
AGCTTGTGAAGGAGCAGAGA+GGG 0.541628 4.4:-41910365 MS.gene021138:CDS
CAGCGTCTTTCAGAGAGATC+CGG 0.542215 4.4:-41910877 MS.gene021138:CDS
GATGGGAAGTGGGTTCTGCA+GGG 0.547301 4.4:-41910199 MS.gene021138:CDS
TTGAGAAAGGGTTATTCGGA+AGG 0.547490 4.4:-41910405 MS.gene021138:CDS
CAGCTTCAGAATAGTTCTGT+TGG 0.547739 4.4:+41910972 None:intergenic
GATGTTCTTCTTTGTAAGGA+TGG 0.548183 4.4:-41911405 MS.gene021138:CDS
AGAGGATTAGGGGAGAAGAG+AGG 0.548379 4.4:-41911537 MS.gene021138:CDS
CTTGAGGCGCGATTATCTGC+TGG 0.549108 4.4:-41911102 MS.gene021138:CDS
TTACTTTGCTGATGGACTTG+AGG 0.550105 4.4:-41911118 MS.gene021138:CDS
GGCCAGAGACTTACAAGCAG+TGG 0.550744 4.4:-41910336 MS.gene021138:CDS
TTCAGCTTCCAAACATCACT+CGG 0.553651 4.4:+41911588 None:intergenic
ACGAAGCCTTGGTGGCCCAC+CGG 0.554118 4.4:+41910858 None:intergenic
TCACAGCTTCTCCATTTCAA+AGG 0.556718 4.4:-41911011 MS.gene021138:CDS
AGTTGATGAGGATGGGAAGT+GGG 0.558093 4.4:-41910209 MS.gene021138:CDS
CGAAGAAAGGTTCCAGTAAG+GGG 0.564272 4.4:-41911293 MS.gene021138:CDS
TAGTTGATGAGGATGGGAAG+TGG 0.564567 4.4:-41910210 MS.gene021138:CDS
CGGATCTCTCTGAAAGACGC+TGG 0.565013 4.4:+41910878 None:intergenic
TAGTGTCCCATTTCTGAGCA+AGG 0.568684 4.4:+41910719 None:intergenic
TGATAGAGAAGAAGTAACAG+TGG 0.569114 4.4:-41910674 MS.gene021138:CDS
TTTGGGTTCTAGAGGATTAG+GGG 0.570677 4.4:-41911547 MS.gene021138:CDS
AGAGACTTACAAGCAGTGGC+AGG 0.571929 4.4:-41910332 MS.gene021138:CDS
CTGCAGCAGACGACGACTGA+AGG 0.573929 4.4:+41911061 None:intergenic
ATTCCTGTAATACGAAGCCT+TGG 0.575936 4.4:+41910847 None:intergenic
TCCATTGGCTCCAGAACTGG+TGG 0.576430 4.4:-41910278 MS.gene021138:CDS
CGTCTTTCAGAGAGATCCGG+TGG 0.576548 4.4:-41910874 MS.gene021138:CDS
AACCTCAACACAGCATCTCT+AGG 0.577064 4.4:+41910595 None:intergenic
AAAGGGTTGAAGAGACAGGC+CGG 0.582129 4.4:-41910792 MS.gene021138:CDS
ATAGGCTGATGATTGAGAAA+GGG 0.583749 4.4:-41910417 MS.gene021138:CDS
TCTCAATGTGCACAAGCTGT+TGG 0.587807 4.4:-41911231 MS.gene021138:CDS
AGGTTAGGAACAAGAGAGCA+AGG 0.590161 4.4:-41910312 MS.gene021138:CDS
GACTTGTGAAGTACTGCAAG+AGG 0.596425 4.4:-41910768 MS.gene021138:CDS
AGAGATCCGGTGGGCCACCA+AGG 0.598231 4.4:-41910864 MS.gene021138:CDS
ATGCATCCGTTAACAGAACC+AGG 0.602016 4.4:+41912053 None:intergenic
CTGTTGGTAGCTATGATCAA+AGG 0.603395 4.4:-41911215 MS.gene021138:CDS
CGGAGAATCGCAATCAACAA+CGG 0.604253 4.4:+41911686 None:intergenic
CGCGTGAAGATGATCAATAT+AGG 0.605875 4.4:-41910435 MS.gene021138:CDS
TGCAGTACTTCACAAGTCGC+CGG 0.605952 4.4:+41910773 None:intergenic
GCAGGCCTAAATCCTGGCTG+AGG 0.614834 4.4:+41910817 None:intergenic
CTGTGTTGAGGTTGATCAGG+AGG 0.616107 4.4:-41910585 MS.gene021138:CDS
AAATACCAAAGTCAATAATG+TGG 0.616394 4.4:+41910929 None:intergenic
GTCCTAGAGATGCTGTGTTG+AGG 0.620188 4.4:-41910597 MS.gene021138:CDS
GTTTGGAAGCTGAATGAAGA+GGG 0.621160 4.4:-41911579 MS.gene021138:CDS
ATGGGACACTATAAGACTTG+AGG 0.623248 4.4:-41910707 MS.gene021138:CDS
TACCAAAGTCAATAATGTGG+AGG 0.623436 4.4:+41910932 None:intergenic
TGATCTCTAACCGGATCATG+AGG 0.623977 4.4:+41911630 None:intergenic
GGTGGAAAGAGTGAAAGAAA+TGG 0.625607 4.4:-41910260 MS.gene021138:CDS
GAAAGACGCTGGATGAGACA+AGG 0.629384 4.4:+41910889 None:intergenic
AGGCAGCATTCTTCGCCAAG+TGG 0.643469 4.4:-41911165 MS.gene021138:CDS
AGAAGATCTGAGAAGTAACA+AGG 0.644638 4.4:-41911475 MS.gene021138:CDS
TGTTTCTGATGAAACGGTGA+CGG 0.654600 4.4:-41910626 MS.gene021138:CDS
GTCTTTCAGAGAGATCCGGT+GGG 0.679118 4.4:-41910873 MS.gene021138:CDS
CTATATTGATCATCTTCACG+CGG 0.683616 4.4:+41910436 None:intergenic
TCCGGAAAATAATGCATACG+AGG 0.702672 4.4:-41911667 MS.gene021138:CDS
GTAAATGTGATAGCTTGTGA+AGG 0.707067 4.4:-41910376 MS.gene021138:CDS
TAGCTTGTGAAGGAGCAGAG+AGG 0.713278 4.4:-41910366 MS.gene021138:CDS
TGTTTGGAAGCTGAATGAAG+AGG 0.744567 4.4:-41911580 MS.gene021138:CDS
GACTGAAGGAGTTTATACAT+CGG 0.748275 4.4:+41911075 None:intergenic
GATCCGGTTAGAGATCAGCG+AGG 0.803140 4.4:-41911624 MS.gene021138:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATATTAGTGATATTCTTA+TGG - chr4.4:41910374-41910393 MS.gene021138:CDS 20.0%
! AAATACCAAAGTCAATAATG+TGG + chr4.4:41911310-41911329 None:intergenic 25.0%
! AATATTCAAACCCTATTCTT+AGG - chr4.4:41910352-41910371 MS.gene021138:CDS 25.0%
! CACTAATATACCTAAGAATA+GGG + chr4.4:41910365-41910384 None:intergenic 25.0%
! GAATTATCACAAAAAATCGA+CGG + chr4.4:41910864-41910883 None:intergenic 25.0%
! TATCAAACAATGATGAGAAA+TGG + chr4.4:41911763-41911782 None:intergenic 25.0%
! TCACTAATATACCTAAGAAT+AGG + chr4.4:41910366-41910385 None:intergenic 25.0%
! TCATCAACTACAAAATCTTT+CGG + chr4.4:41912016-41912035 None:intergenic 25.0%
!! AAAATCGTTTTATGATGTTC+TGG - chr4.4:41910461-41910480 MS.gene021138:CDS 25.0%
!! AAATCGTTTTATGATGTTCT+GGG - chr4.4:41910462-41910481 MS.gene021138:CDS 25.0%
!! AATCCAAACATTTTCATACA+TGG - chr4.4:41911677-41911696 MS.gene021138:CDS 25.0%
!!! ATTGACTTTGGTATTTTCTA+TGG - chr4.4:41911314-41911333 MS.gene021138:CDS 25.0%
AAACAATCTTGCAACATACA+AGG + chr4.4:41910423-41910442 None:intergenic 30.0%
ACACCATGTATGAAAATGTT+TGG + chr4.4:41911683-41911702 None:intergenic 30.0%
ATGATATACTGAAGCAGATT+AGG - chr4.4:41911051-41911070 MS.gene021138:CDS 30.0%
ATGATTGAGAAAGGGTTATT+CGG - chr4.4:41911827-41911846 MS.gene021138:CDS 30.0%
CTGAACCTAGTAAGAAAAAA+GGG + chr4.4:41911728-41911747 None:intergenic 30.0%
GGGGAAAAAATTAAGTACTA+CGG - chr4.4:41910962-41910981 MS.gene021138:CDS 30.0%
GGTTATCTGATGAATGATAA+TGG - chr4.4:41910720-41910739 MS.gene021138:CDS 30.0%
TAAGTACTACGGTAGATTTA+TGG - chr4.4:41910973-41910992 MS.gene021138:CDS 30.0%
TCTTATGGATGAAGAAGATT+TGG - chr4.4:41910389-41910408 MS.gene021138:CDS 30.0%
TGATAAAATTCCTCATGATC+CGG - chr4.4:41910596-41910615 MS.gene021138:CDS 30.0%
! AAATTCGACATCCTTTGAAA+TGG + chr4.4:41911239-41911258 None:intergenic 30.0%
! GAAGAATGTTTCTGATGAAA+CGG - chr4.4:41911604-41911623 MS.gene021138:CDS 30.0%
! TGATGATGTTCTTCTTTGTA+AGG - chr4.4:41910827-41910846 MS.gene021138:CDS 30.0%
! TTGGTTTGAAAAAACAGAGA+CGG + chr4.4:41910223-41910242 None:intergenic 30.0%
!! CTGATGAATGATAATGGTAT+TGG - chr4.4:41910726-41910745 MS.gene021138:CDS 30.0%
!! GAAAGATTTTGTAGTTGATG+AGG - chr4.4:41912015-41912034 MS.gene021138:CDS 30.0%
!!! AATTTTCCTGGTTCTGTTAA+CGG - chr4.4:41910177-41910196 MS.gene021138:CDS 30.0%
!!! ACTTAATTTTTTCCCCTTAC+TGG + chr4.4:41910958-41910977 None:intergenic 30.0%
!!! ATTTTGTAGTTGATGAGGAT+GGG - chr4.4:41912020-41912039 MS.gene021138:CDS 30.0%
!!! CTTTTTTCTTACTAGGTTCA+GGG - chr4.4:41911727-41911746 MS.gene021138:CDS 30.0%
AAGGTTCAAACAACTTCCAT+TGG - chr4.4:41911943-41911962 MS.gene021138:CDS 35.0%
AGAAGATCTGAGAAGTAACA+AGG - chr4.4:41910761-41910780 MS.gene021138:CDS 35.0%
AGTGAAAGAAATGGTGAAGA+AGG - chr4.4:41911985-41912004 MS.gene021138:CDS 35.0%
ATAGGCTGATGATTGAGAAA+GGG - chr4.4:41911819-41911838 MS.gene021138:CDS 35.0%
CCTGAACCTAGTAAGAAAAA+AGG + chr4.4:41911729-41911748 None:intergenic 35.0%
CTATATTGATCATCTTCACG+CGG + chr4.4:41911803-41911822 None:intergenic 35.0%
GACTGAAGGAGTTTATACAT+CGG + chr4.4:41911164-41911183 None:intergenic 35.0%
GATGTTCTTCTTTGTAAGGA+TGG - chr4.4:41910831-41910850 MS.gene021138:CDS 35.0%
GTTTGATATGCTTGAAGCTA+CGG - chr4.4:41911775-41911794 MS.gene021138:CDS 35.0%
TACCAAAGTCAATAATGTGG+AGG + chr4.4:41911307-41911326 None:intergenic 35.0%
TATAGGCTGATGATTGAGAA+AGG - chr4.4:41911818-41911837 MS.gene021138:CDS 35.0%
TCAAAGGATGTCGAATTTCT+TGG - chr4.4:41911241-41911260 MS.gene021138:CDS 35.0%
TGATAGAGAAGAAGTAACAG+TGG - chr4.4:41911562-41911581 MS.gene021138:CDS 35.0%
TGGTTTGAAAAAACAGAGAC+GGG + chr4.4:41910222-41910241 None:intergenic 35.0%
TTAGCTCATTACTTTGCTGA+TGG - chr4.4:41911110-41911129 MS.gene021138:CDS 35.0%
TTCATACATGGTGTAGTGAA+TGG - chr4.4:41911689-41911708 MS.gene021138:CDS 35.0%
! ACACTCGTAGAAAATTTTCC+TGG - chr4.4:41910165-41910184 MS.gene021138:CDS 35.0%
! ACCTCGTATGCATTATTTTC+CGG + chr4.4:41910573-41910592 None:intergenic 35.0%
! CAAATTTGAACGCGTTTTCT+GGG + chr4.4:41910248-41910267 None:intergenic 35.0%
! GTAAATGTGATAGCTTGTGA+AGG - chr4.4:41911860-41911879 MS.gene021138:CDS 35.0%
! GTATTTTCTATGGCTTTCAG+TGG - chr4.4:41911324-41911343 MS.gene021138:CDS 35.0%
! TCAAATTTGAACGCGTTTTC+TGG + chr4.4:41910249-41910268 None:intergenic 35.0%
! TTTGAGGTTATTCCAGAAGA+AGG + chr4.4:41910309-41910328 None:intergenic 35.0%
!!! AGTTTTGTTTTGGGTTCTAG+AGG - chr4.4:41910681-41910700 MS.gene021138:CDS 35.0%
!!! CCTTTTTTCTTACTAGGTTC+AGG - chr4.4:41911726-41911745 MS.gene021138:CDS 35.0%
!!! GAAGAGGGTAGTTTTGTTTT+GGG - chr4.4:41910672-41910691 MS.gene021138:CDS 35.0%
!!! GATTTTGTAGTTGATGAGGA+TGG - chr4.4:41912019-41912038 MS.gene021138:CDS 35.0%
!!! TGAAGAGGGTAGTTTTGTTT+TGG - chr4.4:41910671-41910690 MS.gene021138:CDS 35.0%
!!! TTTTATGATGTTCTGGGATC+CGG - chr4.4:41910468-41910487 MS.gene021138:CDS 35.0%
!!! TTTTGGGTTCTAGAGGATTA+GGG - chr4.4:41910688-41910707 MS.gene021138:CDS 35.0%
ACAATTGCCTTGCTCAGAAA+TGG - chr4.4:41911510-41911529 MS.gene021138:CDS 40.0%
ACTAGAACTAGTTCCAGTTC+CGG + chr4.4:41910490-41910509 None:intergenic 40.0%
AGTGATTCTGTTCCTTCTTC+TGG - chr4.4:41910294-41910313 MS.gene021138:CDS 40.0%
ATCGAAGAAAGGTTCCAGTA+AGG - chr4.4:41910941-41910960 MS.gene021138:CDS 40.0%
ATGGGACACTATAAGACTTG+AGG - chr4.4:41911529-41911548 MS.gene021138:CDS 40.0%
ATTCCTGTAATACGAAGCCT+TGG + chr4.4:41911392-41911411 None:intergenic 40.0%
CAATTGCCTTGCTCAGAAAT+GGG - chr4.4:41911511-41911530 MS.gene021138:CDS 40.0%
CGCGTGAAGATGATCAATAT+AGG - chr4.4:41911801-41911820 MS.gene021138:CDS 40.0%
CTCAGAAGATTCAACGTTTG+AGG + chr4.4:41910325-41910344 None:intergenic 40.0%
GAAGGAACAGAATCACTTGA+AGG + chr4.4:41910291-41910310 None:intergenic 40.0%
GCAAGGCAATTGTACTCAAA+TGG + chr4.4:41911503-41911522 None:intergenic 40.0%
GGTGGAAAGAGTGAAAGAAA+TGG - chr4.4:41911976-41911995 MS.gene021138:CDS 40.0%
GTTTGGAAGCTGAATGAAGA+GGG - chr4.4:41910657-41910676 MS.gene021138:CDS 40.0%
TCACAGCTTCTCCATTTCAA+AGG - chr4.4:41911225-41911244 MS.gene021138:CDS 40.0%
TCCGGAAAATAATGCATACG+AGG - chr4.4:41910569-41910588 MS.gene021138:CDS 40.0%
TCGAAGAAAGGTTCCAGTAA+GGG - chr4.4:41910942-41910961 MS.gene021138:CDS 40.0%
TGAGAAAGGGTTATTCGGAA+GGG - chr4.4:41911832-41911851 MS.gene021138:CDS 40.0%
TGTTTGGAAGCTGAATGAAG+AGG - chr4.4:41910656-41910675 MS.gene021138:CDS 40.0%
TTCAGCTTCCAAACATCACT+CGG + chr4.4:41910651-41910670 None:intergenic 40.0%
TTGAGAAAGGGTTATTCGGA+AGG - chr4.4:41911831-41911850 MS.gene021138:CDS 40.0%
! CAGCTTCAGAATAGTTCTGT+TGG + chr4.4:41911267-41911286 None:intergenic 40.0%
! TCTGGTTCAGGAAGATCTAA+TGG - chr4.4:41910906-41910925 MS.gene021138:CDS 40.0%
! TCTGTTAACGGATGCATGTT+TGG - chr4.4:41910189-41910208 MS.gene021138:CDS 40.0%
! TGTTTCTGATGAAACGGTGA+CGG - chr4.4:41911610-41911629 MS.gene021138:CDS 40.0%
!! AGCCTCCACATTATTGACTT+TGG - chr4.4:41911302-41911321 MS.gene021138:CDS 40.0%
!! CAACAGAACTATTCTGAAGC+TGG - chr4.4:41911265-41911284 MS.gene021138:CDS 40.0%
!! CAATCACAGATTTCTGGTTC+AGG - chr4.4:41910894-41910913 MS.gene021138:CDS 40.0%
!! CTGTTGGTAGCTATGATCAA+AGG - chr4.4:41911021-41911040 MS.gene021138:CDS 40.0%
!! TTACTTTGCTGATGGACTTG+AGG - chr4.4:41911118-41911137 MS.gene021138:CDS 40.0%
!! TTTGGGTTCTAGAGGATTAG+GGG - chr4.4:41910689-41910708 MS.gene021138:CDS 40.0%
!!! ACGCACCCTTTTTTCTTACT+AGG - chr4.4:41911720-41911739 MS.gene021138:CDS 40.0%
!!! AGAGATCAGCGAGGTTTTTT+CGG - chr4.4:41910621-41910640 MS.gene021138:CDS 40.0%
!!! GTTTTGGGTTCTAGAGGATT+AGG - chr4.4:41910687-41910706 MS.gene021138:CDS 40.0%
AAACCTCGCTGATCTCTAAC+CGG + chr4.4:41910618-41910637 None:intergenic 45.0%
AACCTCAACACAGCATCTCT+AGG + chr4.4:41911644-41911663 None:intergenic 45.0%
AAGACATCGCGATCGAAGAA+AGG - chr4.4:41910930-41910949 MS.gene021138:CDS 45.0%
AGGATTTAGGCCTGCAGAAA+GGG - chr4.4:41911427-41911446 MS.gene021138:CDS 45.0%
AGGTTAGGAACAAGAGAGCA+AGG - chr4.4:41911924-41911943 MS.gene021138:CDS 45.0%
ATCTTCCTCAGCCAGGATTT+AGG - chr4.4:41911414-41911433 MS.gene021138:CDS 45.0%
ATGCATCCGTTAACAGAACC+AGG + chr4.4:41910186-41910205 None:intergenic 45.0%
CGAAGAAAGGTTCCAGTAAG+GGG - chr4.4:41910943-41910962 MS.gene021138:CDS 45.0%
CGGAGAATCGCAATCAACAA+CGG + chr4.4:41910553-41910572 None:intergenic 45.0%
TAGTGTCCCATTTCTGAGCA+AGG + chr4.4:41911520-41911539 None:intergenic 45.0%
TCGTCGCAATCACAGATTTC+TGG - chr4.4:41910888-41910907 MS.gene021138:CDS 45.0%
TCTCAATGTGCACAAGCTGT+TGG - chr4.4:41911005-41911024 MS.gene021138:CDS 45.0%
TGATCTCTAACCGGATCATG+AGG + chr4.4:41910609-41910628 None:intergenic 45.0%
TTCACTCTTTCCACCAGTTC+TGG + chr4.4:41911971-41911990 None:intergenic 45.0%
! ATGCTGTGTTGAGGTTGATC+AGG - chr4.4:41911648-41911667 MS.gene021138:CDS 45.0%
! CGTTGTTGATTGCGATTCTC+CGG - chr4.4:41910551-41910570 MS.gene021138:CDS 45.0%
! TCGGTTTTCCGAGTGATGTT+TGG - chr4.4:41910640-41910659 MS.gene021138:CDS 45.0%
! TGAACGCGTTTTCTGGGTTT+TGG + chr4.4:41910242-41910261 None:intergenic 45.0%
!! AGTTGATGAGGATGGGAAGT+GGG - chr4.4:41912027-41912046 MS.gene021138:CDS 45.0%
!! GACTTGTGAAGTACTGCAAG+AGG - chr4.4:41911468-41911487 MS.gene021138:CDS 45.0%
!! GTGAAGTACTGCAAGAGGTT+TGG - chr4.4:41911473-41911492 MS.gene021138:CDS 45.0%
!! TAGTTGATGAGGATGGGAAG+TGG - chr4.4:41912026-41912045 MS.gene021138:CDS 45.0%
!! TCTGCTAGCTGTTTCTTGCT+GGG - chr4.4:41912075-41912094 MS.gene021138:CDS 45.0%
!! TTCTGCTAGCTGTTTCTTGC+TGG - chr4.4:41912074-41912093 MS.gene021138:CDS 45.0%
AAAGGGTTGAAGAGACAGGC+CGG - chr4.4:41911444-41911463 MS.gene021138:CDS 50.0%
AAGGAGCAGAGAGGGTTGAA+AGG - chr4.4:41911879-41911898 MS.gene021138:CDS 50.0%
AGAGACTTACAAGCAGTGGC+AGG - chr4.4:41911904-41911923 MS.gene021138:CDS 50.0%
AGAGGATTAGGGGAGAAGAG+AGG - chr4.4:41910699-41910718 MS.gene021138:CDS 50.0%
AGCTTGTGAAGGAGCAGAGA+GGG - chr4.4:41911871-41911890 MS.gene021138:CDS 50.0%
CAGCGTCTTTCAGAGAGATC+CGG - chr4.4:41911359-41911378 MS.gene021138:CDS 50.0%
CAGGATTTAGGCCTGCAGAA+AGG - chr4.4:41911426-41911445 MS.gene021138:CDS 50.0%
CCACCAAGGCTTCGTATTAC+AGG - chr4.4:41911386-41911405 MS.gene021138:CDS 50.0%
CCTGTAATACGAAGCCTTGG+TGG + chr4.4:41911389-41911408 None:intergenic 50.0%
CTTACAAGCAGTGGCAGGTT+AGG - chr4.4:41911909-41911928 MS.gene021138:CDS 50.0%
CTTTCTGCAGGCCTAAATCC+TGG + chr4.4:41911428-41911447 None:intergenic 50.0%
GAAAGACGCTGGATGAGACA+AGG + chr4.4:41911350-41911369 None:intergenic 50.0%
GCAGAAAGGGTTGAAGAGAC+AGG - chr4.4:41911440-41911459 MS.gene021138:CDS 50.0%
GGAATTGATCTTCCTCAGCC+AGG - chr4.4:41911407-41911426 MS.gene021138:CDS 50.0%
GTCCTAGAGATGCTGTGTTG+AGG - chr4.4:41911639-41911658 MS.gene021138:CDS 50.0%
GTCTCTTCAACCCTTTCTGC+AGG + chr4.4:41911440-41911459 None:intergenic 50.0%
GTCTTTCAGAGAGATCCGGT+GGG - chr4.4:41911363-41911382 MS.gene021138:CDS 50.0%
TGCAGTACTTCACAAGTCGC+CGG + chr4.4:41911466-41911485 None:intergenic 50.0%
TGCCACTGCTTGTAAGTCTC+TGG + chr4.4:41911905-41911924 None:intergenic 50.0%
! CTGTGTTGAGGTTGATCAGG+AGG - chr4.4:41911651-41911670 MS.gene021138:CDS 50.0%
! TAGCTTGTGAAGGAGCAGAG+AGG - chr4.4:41911870-41911889 MS.gene021138:CDS 50.0%
!! ACTTCCATTGGCTCCAGAAC+TGG - chr4.4:41911955-41911974 MS.gene021138:CDS 50.0%
AGGCAGCATTCTTCGCCAAG+TGG - chr4.4:41911071-41911090 MS.gene021138:CDS 55.0%
CATTCTTCGCCAAGTGGCGA+TGG - chr4.4:41911077-41911096 MS.gene021138:CDS 55.0%
CGTCTTTCAGAGAGATCCGG+TGG - chr4.4:41911362-41911381 MS.gene021138:CDS 55.0%
CTTGAGGCGCGATTATCTGC+TGG - chr4.4:41911134-41911153 MS.gene021138:CDS 55.0%
GATCCGGTTAGAGATCAGCG+AGG - chr4.4:41910612-41910631 MS.gene021138:CDS 55.0%
GGCCAGAGACTTACAAGCAG+TGG - chr4.4:41911900-41911919 MS.gene021138:CDS 55.0%
GGGTTCTGCAGGGTTGGAAA+GGG - chr4.4:41912047-41912066 MS.gene021138:CDS 55.0%
TGGGTTCTGCAGGGTTGGAA+AGG - chr4.4:41912046-41912065 MS.gene021138:CDS 55.0%
! CGGATCTCTCTGAAAGACGC+TGG + chr4.4:41911361-41911380 None:intergenic 55.0%
! GATGGGAAGTGGGTTCTGCA+GGG - chr4.4:41912037-41912056 MS.gene021138:CDS 55.0%
!! GATGTTCTGGGATCCGGAAC+TGG - chr4.4:41910474-41910493 MS.gene021138:CDS 55.0%
!! TCCACCAGTTCTGGAGCCAA+TGG + chr4.4:41911962-41911981 None:intergenic 55.0%
!! TCCATTGGCTCCAGAACTGG+TGG - chr4.4:41911958-41911977 MS.gene021138:CDS 55.0%
CGCTGAAGTCCATCGCCACT+TGG + chr4.4:41911089-41911108 None:intergenic 60.0%
CTGCAGCAGACGACGACTGA+AGG + chr4.4:41911178-41911197 None:intergenic 60.0%
GCAGGCCTAAATCCTGGCTG+AGG + chr4.4:41911422-41911441 None:intergenic 60.0%
! GGAAGTGGGTTCTGCAGGGT+TGG - chr4.4:41912041-41912060 MS.gene021138:CDS 60.0%
! GGATGGGAAGTGGGTTCTGC+AGG - chr4.4:41912036-41912055 MS.gene021138:CDS 60.0%
AGAGATCCGGTGGGCCACCA+AGG - chr4.4:41911372-41911391 MS.gene021138:CDS 65.0%
! ACGAAGCCTTGGTGGCCCAC+CGG + chr4.4:41911381-41911400 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 41910150 41912108 41910150 ID=MS.gene021138
chr4.4 mRNA 41910150 41912108 41910150 ID=MS.gene021138.t1;Parent=MS.gene021138
chr4.4 exon 41910150 41912108 41910150 ID=MS.gene021138.t1.exon1;Parent=MS.gene021138.t1
chr4.4 CDS 41910150 41912108 41910150 ID=cds.MS.gene021138.t1;Parent=MS.gene021138.t1
Gene Sequence

>MS.gene021138

ATGTTTTCTACAGAAACACTCGTAGAAAATTTTCCTGGTTCTGTTAACGGATGCATGTTTGGAAACATACCCGTCTCTGTTTTTTCAAACCAAAACCCAGAAAACGCGTTCAAATTTGAAGATTCAAATTCAACTTCACCTTCAAGTGATTCTGTTCCTTCTTCTGGAATAACCTCAAACGTTGAATCTTCTGAGAGTAGTAAATATTCAAACCCTATTCTTAGGTATATTAGTGATATTCTTATGGATGAAGAAGATTTGGAGACAAAACCTTGTATGTTGCAAGATTGTTTGAAGCTTCAAGCTGCTGAAAAATCGTTTTATGATGTTCTGGGATCCGGAACTGGAACTAGTTCTAGTTCTGAGAGTTATAGTAATTGTAGTTATAATACTGATAATTCCGTTGTTGATTGCGATTCTCCGGAAAATAATGCATACGAGGTGTTTGATAAAATTCCTCATGATCCGGTTAGAGATCAGCGAGGTTTTTTCGGTTTTCCGAGTGATGTTTGGAAGCTGAATGAAGAGGGTAGTTTTGTTTTGGGTTCTAGAGGATTAGGGGAGAAGAGAGGTTATCTGATGAATGATAATGGTATTGGTATTTCACATGAAGAAGATCTGAGAAGTAACAAGGTTTCAGCTGTTTACTATGATGATACAGAGTTATCAGAAATGTTTGATGATGTTCTTCTTTGTAAGGATGGTAAAAGTCCGTCGATTTTTTGTGATAATTCTGAGTCGTCGCAATCACAGATTTCTGGTTCAGGAAGATCTAATGGAAAGACATCGCGATCGAAGAAAGGTTCCAGTAAGGGGAAAAAATTAAGTACTACGGTAGATTTATGGACTTTGTTATCTCAATGTGCACAAGCTGTTGGTAGCTATGATCAAAGGAATGCAAATGATATACTGAAGCAGATTAGGCAGCATTCTTCGCCAAGTGGCGATGGACTTCAGCGTTTAGCTCATTACTTTGCTGATGGACTTGAGGCGCGATTATCTGCTGGTACACCGATGTATAAACTCCTTCAGTCGTCGTCTGCTGCAGATATGTTGAGAGCTCATAAAGTTTATATCACAGCTTCTCCATTTCAAAGGATGTCGAATTTCTTGGCCAACAGAACTATTCTGAAGCTGGTAGAAAATAAAAGCAGCCTCCACATTATTGACTTTGGTATTTTCTATGGCTTTCAGTGGCCTTGTCTCATCCAGCGTCTTTCAGAGAGATCCGGTGGGCCACCAAGGCTTCGTATTACAGGAATTGATCTTCCTCAGCCAGGATTTAGGCCTGCAGAAAGGGTTGAAGAGACAGGCCGGCGACTTGTGAAGTACTGCAAGAGGTTTGGAGTTCCATTTGAGTACAATTGCCTTGCTCAGAAATGGGACACTATAAGACTTGAGGATCTCAAGATTGATAGAGAAGAAGTAACAGTGGTTAACTGCTTACACAGATTGAAGAATGTTTCTGATGAAACGGTGACGGCGAATTGTCCTAGAGATGCTGTGTTGAGGTTGATCAGGAGGATTAATCCAAACATTTTCATACATGGTGTAGTGAATGGAACTTATAACGCACCCTTTTTTCTTACTAGGTTCAGGGAAGCACTATTCCATTTCTCATCATTGTTTGATATGCTTGAAGCTACGGTGCCGCGTGAAGATGATCAATATAGGCTGATGATTGAGAAAGGGTTATTCGGAAGGGATGCAGTAAATGTGATAGCTTGTGAAGGAGCAGAGAGGGTTGAAAGGCCAGAGACTTACAAGCAGTGGCAGGTTAGGAACAAGAGAGCAAGGTTCAAACAACTTCCATTGGCTCCAGAACTGGTGGAAAGAGTGAAAGAAATGGTGAAGAAGGAGTATCCGAAAGATTTTGTAGTTGATGAGGATGGGAAGTGGGTTCTGCAGGGTTGGAAAGGGCGTATTCTGCTAGCTGTTTCTTGCTGGGTTCCTGCTTAA
Protein sequence

>MS.gene021138.t1

MFSTETLVENFPGSVNGCMFGNIPVSVFSNQNPENAFKFEDSNSTSPSSDSVPSSGITSNVESSESSKYSNPILRYISDILMDEEDLETKPCMLQDCLKLQAAEKSFYDVLGSGTGTSSSSESYSNCSYNTDNSVVDCDSPENNAYEVFDKIPHDPVRDQRGFFGFPSDVWKLNEEGSFVLGSRGLGEKRGYLMNDNGIGISHEEDLRSNKVSAVYYDDTELSEMFDDVLLCKDGKSPSIFCDNSESSQSQISGSGRSNGKTSRSKKGSSKGKKLSTTVDLWTLLSQCAQAVGSYDQRNANDILKQIRQHSSPSGDGLQRLAHYFADGLEARLSAGTPMYKLLQSSSAADMLRAHKVYITASPFQRMSNFLANRTILKLVENKSSLHIIDFGIFYGFQWPCLIQRLSERSGGPPRLRITGIDLPQPGFRPAERVEETGRRLVKYCKRFGVPFEYNCLAQKWDTIRLEDLKIDREEVTVVNCLHRLKNVSDETVTANCPRDAVLRLIRRINPNIFIHGVVNGTYNAPFFLTRFREALFHFSSLFDMLEATVPREDDQYRLMIEKGLFGRDAVNVIACEGAERVERPETYKQWQVRNKRARFKQLPLAPELVERVKEMVKKEYPKDFVVDEDGKWVLQGWKGRILLAVSCWVPA