AlfalfaGEDB | MS.gene021882

M. sativa cultivar XinJiangDaYe / MS.gene021882

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021882.t1	AES77659.2	69.6	138	42	0	1	138	1	138	3.30E-46	194.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021882.t1	O48686	37.8	119	68	3	14	130	15	129	4.1e-13	75.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021882.t1	G7L5A9	69.6	138	42	0	1	138	1	138	2.4e-46	194.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene021882.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021882.t1	MTR_7g013380	74.803	127	32	0	12	138	98	224	1.35e-59	186
MS.gene021882.t1	MTR_7g013380	69.565	138	42	0	1	138	1	138	1.65e-54	173
MS.gene021882.t1	MTR_7g013380	72.941	85	23	0	6	90	178	262	2.23e-37	129
MS.gene021882.t1	MTR_8g062700	43.269	104	55	2	29	130	56	157	2.56e-19	83.2
MS.gene021882.t1	MTR_8g062700	43.269	104	55	2	29	130	56	157	2.56e-19	83.2
MS.gene021882.t1	MTR_3g006125	38.281	128	71	3	14	135	90	215	1.92e-17	76.3
MS.gene021882.t1	MTR_5g036670	40.196	102	57	2	31	130	59	158	2.57e-15	72.0
MS.gene021882.t1	MTR_3g006105	35.156	128	75	3	14	135	9	134	2.61e-15	69.3
MS.gene021882.t1	MTR_6g008995	35.897	117	71	2	14	130	38	150	7.93e-15	70.5
MS.gene021882.t1	MTR_3g006185	35.294	136	77	4	14	140	9	142	1.64e-14	67.0
MS.gene021882.t1	MTR_1g104937	42.308	78	43	1	13	90	12	87	6.21e-14	63.9
MS.gene021882.t1	MTR_1g104960	33.065	124	77	4	14	135	14	133	2.34e-13	63.9
MS.gene021882.t1	MTR_3g006150	33.333	114	74	2	26	138	16	128	6.41e-13	62.4
MS.gene021882.t1	MTR_6g008970	36.975	119	69	4	14	130	64	178	7.85e-13	64.7
MS.gene021882.t1	MTR_3g006065	35.185	108	68	2	32	138	22	128	8.98e-13	62.8
MS.gene021882.t1	MTR_6g008995	36.170	94	58	1	37	130	2	93	7.52e-12	61.6
MS.gene021882.t1	MTR_1g104940	41.892	74	41	2	17	90	18	89	7.52e-12	58.9
MS.gene021882.t1	MTR_1g105010	41.558	77	43	2	14	90	17	91	1.67e-11	57.8
MS.gene021882.t1	MTR_4g133300	31.776	107	67	2	26	132	20	120	2.25e-11	59.7
MS.gene021882.t1	MTR_4g133300	32.283	127	81	4	7	133	83	204	7.53e-11	58.2
MS.gene021882.t1	MTR_7g013410	29.464	112	72	3	17	128	2	106	3.41e-11	57.4
MS.gene021882.t1	MTR_1g104980	33.607	122	60	5	16	135	2	104	4.25e-11	58.2
MS.gene021882.t1	MTR_4g133470	30.841	107	68	2	26	132	20	120	9.58e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021882.t1	AT1G24190	36.923	130	76	4	3	130	4	129	2.58e-16	74.7
MS.gene021882.t1	AT1G24190	36.923	130	76	4	3	130	4	129	2.71e-16	74.7
MS.gene021882.t1	AT1G24190	36.923	130	76	4	3	130	4	129	2.84e-16	74.7
MS.gene021882.t1	AT1G70060	36.641	131	76	4	3	130	4	130	7.70e-16	73.6
MS.gene021882.t1	AT1G70060	36.641	131	76	4	3	130	4	130	7.70e-16	73.6
MS.gene021882.t1	AT5G15020	40.171	117	66	4	14	130	53	165	1.49e-14	69.7
MS.gene021882.t1	AT5G15020	40.171	117	66	4	14	130	53	165	1.52e-14	69.7
MS.gene021882.t1	AT1G59890	36.752	117	70	3	14	130	46	158	7.26e-14	67.8
MS.gene021882.t1	AT3G01320	38.655	119	66	4	14	130	58	171	7.34e-14	67.8
MS.gene021882.t1	AT1G59890	36.752	117	70	3	14	130	46	158	7.47e-14	67.8
MS.gene021882.t1	AT1G59890	36.752	117	70	3	14	130	46	158	7.69e-14	67.8
MS.gene021882.t1	AT3G01320	38.655	119	66	4	14	130	58	171	7.92e-14	67.8
MS.gene021882.t1	AT1G59890	36.752	117	70	3	14	130	46	158	8.97e-14	67.4
MS.gene021882.t1	AT1G70030	34.921	126	76	5	14	138	12	132	1.76e-13	63.9
MS.gene021882.t1	AT1G10450	36.000	100	62	1	31	130	100	197	2.83e-12	63.2
MS.gene021882.t1	AT1G10450	36.000	100	62	1	31	130	49	146	3.95e-12	62.8
MS.gene021882.t1	AT1G10450	36.000	100	62	1	31	130	49	146	3.95e-12	62.8
MS.gene021882.t1	AT1G24200	32.231	121	77	3	14	134	15	130	2.29e-11	58.9

Find 23 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTCATTGACAAATTCTT+TGG	0.276334	7.1:-82443129	None:intergenic
ATAGATTTATTCAAGATAAC+AGG	0.349948	7.1:+82443525	MS.gene021882:CDS
GGAACATTGTGAAGCCAAAA+AGG	0.355327	7.1:+82443106	MS.gene021882:CDS
TGACTTTCTGAAAGTCTTGA+AGG	0.381266	7.1:+82443562	MS.gene021882:CDS
AACTTCAATGACAAATTCTT+CGG	0.410704	7.1:-82443498	None:intergenic
ATCGGAGGAGGAACAATCTC+TGG	0.440798	7.1:+82443454	MS.gene021882:CDS
TGATGACTTTCTGAAAGTCT+TGG	0.476784	7.1:+82443190	MS.gene021882:CDS
AGCTTGATTTCATATCCATC+TGG	0.477967	7.1:-82443428	None:intergenic
GAATCTCGGTAAAATATTGA+AGG	0.498251	7.1:+82443355	MS.gene021882:CDS
TGACTTTCTGAAAGTCTTGG+AGG	0.503843	7.1:+82443193	MS.gene021882:CDS
ATATTGAAGGAATTATTTGA+AGG	0.515550	7.1:+82443368	MS.gene021882:CDS
AGGATTGATGCTGCGAATCT+CGG	0.525928	7.1:+82443341	MS.gene021882:intron
GACTTTCTGAAAGTCTTGGA+GGG	0.543760	7.1:+82443194	MS.gene021882:CDS
AGAGATTGTTCCTCCTCCGA+TGG	0.550257	7.1:-82443452	None:intergenic
TTGAAATACTACATACCAGA+TGG	0.567499	7.1:+82443413	MS.gene021882:CDS
AATCAAGCTTCCATCGGAGG+AGG	0.577026	7.1:+82443442	MS.gene021882:CDS
GATTTATTCAAGATAACAGG+TGG	0.581810	7.1:+82443528	MS.gene021882:CDS
TGAAGGATTATATGACACAA+AGG	0.612910	7.1:+82443579	MS.gene021882:CDS
TATCAACCCGAGATAATGAA+AGG	0.613277	7.1:+82443074	None:intergenic
TGAAATCAAGCTTCCATCGG+AGG	0.622504	7.1:+82443439	MS.gene021882:CDS
TGGAGGGTTACAAGACACAA+AGG	0.650188	7.1:+82443210	MS.gene021882:CDS
ATATGAAATCAAGCTTCCAT+CGG	0.662074	7.1:+82443436	MS.gene021882:CDS
ATTTATTCAAGATAACAGGT+GGG	0.670883	7.1:+82443529	MS.gene021882:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTATTATATTGTTAGA+TGG	+	chr7.1:82443261-82443280	MS.gene021882:intron	10.0%
!!	ATAGATTTATTCAAGATAAC+AGG	+	chr7.1:82443525-82443544	MS.gene021882:CDS	20.0%
!!	ATATTGAAGGAATTATTTGA+AGG	+	chr7.1:82443368-82443387	MS.gene021882:CDS	20.0%
!!!	ATCTAATTTATCTATCCTTT+TGG	+	chr7.1:82443308-82443327	MS.gene021882:intron	20.0%
!!!	TTTTTTTAATTCTCACAAGA+TGG	+	chr7.1:82443602-82443621	MS.gene021882:intron	20.0%
!	AACTTCAATGACAAATTCTT+CGG	-	chr7.1:82443501-82443520	None:intergenic	25.0%
!	AACTTCATTGACAAATTCTT+TGG	-	chr7.1:82443132-82443151	None:intergenic	25.0%
!	ATTTATTCAAGATAACAGGT+GGG	+	chr7.1:82443529-82443548	MS.gene021882:CDS	25.0%
	ATATGAAATCAAGCTTCCAT+CGG	+	chr7.1:82443436-82443455	MS.gene021882:CDS	30.0%
	ATCCTACAAAATGTACCAAA+AGG	-	chr7.1:82443326-82443345	None:intergenic	30.0%
	GAATCTCGGTAAAATATTGA+AGG	+	chr7.1:82443355-82443374	MS.gene021882:CDS	30.0%
	GATTTATTCAAGATAACAGG+TGG	+	chr7.1:82443528-82443547	MS.gene021882:CDS	30.0%
	TGAAGGATTATATGACACAA+AGG	+	chr7.1:82443579-82443598	MS.gene021882:CDS	30.0%
	TTGAAATACTACATACCAGA+TGG	+	chr7.1:82443413-82443432	MS.gene021882:CDS	30.0%
!	ATCCTTTTGGTACATTTTGT+AGG	+	chr7.1:82443321-82443340	MS.gene021882:intron	30.0%
	AGCTTGATTTCATATCCATC+TGG	-	chr7.1:82443431-82443450	None:intergenic	35.0%
	CCTGAAGATCAATGACAAAA+TGG	-	chr7.1:82443666-82443685	None:intergenic	35.0%
!	CCATTTTGTCATTGATCTTC+AGG	+	chr7.1:82443663-82443682	MS.gene021882:intron	35.0%
!	GACAAATTCTTCGGCATTTT+TGG	-	chr7.1:82443492-82443511	None:intergenic	35.0%
!	TGACTTTCTGAAAGTCTTGA+AGG	+	chr7.1:82443562-82443581	MS.gene021882:CDS	35.0%
!	TGATGACTTTCTGAAAGTCT+TGG	+	chr7.1:82443190-82443209	MS.gene021882:CDS	35.0%
!!	GACAAATTCTTTGGCCTTTT+TGG	-	chr7.1:82443123-82443142	None:intergenic	35.0%
	GGAACATTGTGAAGCCAAAA+AGG	+	chr7.1:82443106-82443125	MS.gene021882:CDS	40.0%
	TTCTCACAAGATGGTGCAAA+AGG	+	chr7.1:82443611-82443630	MS.gene021882:intron	40.0%
	TTGTCATTGATCTTCAGGTG+TGG	+	chr7.1:82443668-82443687	MS.gene021882:intron	40.0%
!	GACTTTCTGAAAGTCTTGGA+GGG	+	chr7.1:82443194-82443213	MS.gene021882:CDS	40.0%
!	TGACTTTCTGAAAGTCTTGG+AGG	+	chr7.1:82443193-82443212	MS.gene021882:CDS	40.0%
	AGGATTGATGCTGCGAATCT+CGG	+	chr7.1:82443341-82443360	MS.gene021882:intron	45.0%
	CACAAGATGGTGCAAAAGGA+TGG	+	chr7.1:82443615-82443634	MS.gene021882:intron	45.0%
	TGAAATCAAGCTTCCATCGG+AGG	+	chr7.1:82443439-82443458	MS.gene021882:CDS	45.0%
	TGGAGGGTTACAAGACACAA+AGG	+	chr7.1:82443210-82443229	MS.gene021882:CDS	45.0%
!!	TAATAAAAAAAATAAATTAG+TGG	-	chr7.1:82443251-82443270	None:intergenic	5.0%
	AATCAAGCTTCCATCGGAGG+AGG	+	chr7.1:82443442-82443461	MS.gene021882:CDS	50.0%
	AGAGATTGTTCCTCCTCCGA+TGG	-	chr7.1:82443455-82443474	None:intergenic	50.0%
	ATCGGAGGAGGAACAATCTC+TGG	+	chr7.1:82443454-82443473	MS.gene021882:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	82443089	82443706	82443089	ID=MS.gene021882
chr7.1	mRNA	82443089	82443706	82443089	ID=MS.gene021882.t1;Parent=MS.gene021882
chr7.1	exon	82443089	82443231	82443089	ID=MS.gene021882.t1.exon1;Parent=MS.gene021882.t1
chr7.1	CDS	82443089	82443231	82443089	ID=cds.MS.gene021882.t1;Parent=MS.gene021882.t1
chr7.1	exon	82443343	82443600	82443343	ID=MS.gene021882.t1.exon2;Parent=MS.gene021882.t1
chr7.1	CDS	82443343	82443600	82443343	ID=cds.MS.gene021882.t1;Parent=MS.gene021882.t1
chr7.1	exon	82443685	82443706	82443685	ID=MS.gene021882.t1.exon3;Parent=MS.gene021882.t1
chr7.1	CDS	82443685	82443706	82443685	ID=cds.MS.gene021882.t1;Parent=MS.gene021882.t1

Gene Sequence

>MS.gene021882

ATGAAAGGACATTTTCTGGAACATTGTGAAGCCAAAAAGGCCAAAGAATTTGTCAATGAAGTTAAGTGTAGATTTATTCAAGATAACATGAGAGATAAGTATGATGACTTTCTGAAAGTCTTGGAGGGTTACAAGACACAAAGGTTTGATCATGTGTGACCACTAATTTATTTTTTTTATTATATTGTTAGATGGATTTTATTAAATATTGTTGATTGTATCTAATTTATCTATCCTTTTGGTACATTTTGTAGGATTGATGCTGCGAATCTCGGTAAAATATTGAAGGAATTATTTGAAGGTCATAGAGATCTTATATCAAGATTGAAATACTACATACCAGATGGATATGAAATCAAGCTTCCATCGGAGGAGGAACAATCTCTGGAACATTGCGAAGCCAAAAATGCCGAAGAATTTGTCATTGAAGTTAAACATAGATTTATTCAAGATAACAGGTGGGATATGTATGATGACTTTCTGAAAGTCTTGAAGGATTATATGACACAAAGGTTTTTTTAATTCTCACAAGATGGTGCAAAAGGATGGCATATTGCATATACTAAATGTTGTTCCATTTTGTCATTGATCTTCAGGTGTGGTGCAGCAAGATCTTAA

Protein sequence

>MS.gene021882.t1

MKGHFLEHCEAKKAKEFVNEVKCRFIQDNMRDKYDDFLKVLEGYKTQRIDAANLGKILKELFEGHRDLISRLKYYIPDGYEIKLPSEEEQSLEHCEAKNAEEFVIEVKHRFIQDNRWDMYDDFLKVLKDYMTQRCGAARS