AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021927


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021927.t1 MTR_7g015010 97.151 351 10 0 1 351 1 351 0.0 697
MS.gene021927.t1 MTR_6g015350 42.973 370 154 16 1 351 2 333 1.45e-62 203
MS.gene021927.t1 MTR_4g109660 62.185 119 42 2 1 118 1 117 3.71e-45 157
MS.gene021927.t1 MTR_3g026050 44.783 230 101 9 1 209 1 225 1.67e-44 157
MS.gene021927.t1 MTR_8g463380 60.526 76 30 0 51 126 48 123 2.56e-24 103
MS.gene021927.t1 MTR_4g079580 58.442 77 32 0 51 127 16 92 6.87e-24 100
MS.gene021927.t1 MTR_2g078200 61.644 73 28 0 51 123 14 86 9.70e-24 99.8
MS.gene021927.t1 MTR_2g090960 60.811 74 29 0 51 124 18 91 2.92e-23 99.0
MS.gene021927.t1 MTR_4g111935 31.330 233 127 9 58 264 133 358 3.58e-15 76.3
MS.gene021927.t1 MTR_6g017055 39.623 106 58 3 58 158 127 231 9.46e-14 72.0
MS.gene021927.t1 MTR_7g018500 50.877 57 28 0 59 115 122 178 4.65e-13 70.1
MS.gene021927.t1 MTR_1g103380 48.333 60 31 0 56 115 200 259 1.10e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021927.t1 AT3G02150 75.000 100 25 0 30 129 47 146 1.66e-45 157
MS.gene021927.t1 AT3G02150 75.000 100 25 0 30 129 47 146 4.05e-45 158
MS.gene021927.t1 AT5G08070 71.717 99 24 2 25 123 4 98 5.56e-42 147
MS.gene021927.t1 AT5G08070 71.717 99 24 2 25 123 4 98 5.56e-42 147
MS.gene021927.t1 AT5G60970 83.750 80 12 1 43 122 49 127 6.14e-42 150
MS.gene021927.t1 AT1G30210 75.000 72 18 0 47 118 40 111 9.20e-30 116
MS.gene021927.t1 AT1G30210 75.000 72 18 0 47 118 40 111 9.20e-30 116
MS.gene021927.t1 AT4G18390 35.000 260 112 8 47 298 32 242 3.00e-27 110
MS.gene021927.t1 AT4G18390 35.000 260 112 8 47 298 32 242 3.00e-27 110
MS.gene021927.t1 AT1G53230 61.842 76 29 0 51 126 43 118 2.06e-25 105
MS.gene021927.t1 AT1G53230 61.842 76 29 0 51 126 43 118 2.06e-25 105
MS.gene021927.t1 AT3G15030 60.811 74 29 0 51 124 39 112 9.62e-24 101
MS.gene021927.t1 AT3G15030 60.811 74 29 0 51 124 39 112 9.62e-24 101
MS.gene021927.t1 AT3G15030 60.811 74 29 0 51 124 39 112 9.62e-24 101
MS.gene021927.t1 AT3G15030 60.811 74 29 0 51 124 39 112 9.62e-24 101
MS.gene021927.t1 AT2G31070 64.615 65 23 0 51 115 23 87 5.33e-21 93.2
MS.gene021927.t1 AT1G68800 47.761 67 35 0 49 115 104 170 5.49e-15 75.5
MS.gene021927.t1 AT1G68800 47.761 67 35 0 49 115 104 170 5.92e-15 75.5
MS.gene021927.t1 AT1G67260 42.105 76 44 0 46 121 76 151 6.28e-15 75.5
MS.gene021927.t1 AT1G67260 42.105 76 44 0 46 121 76 151 6.28e-15 75.5
MS.gene021927.t1 AT1G67260 42.105 76 44 0 46 121 76 151 7.01e-15 75.5
MS.gene021927.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.25e-13 72.0
MS.gene021927.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.39e-13 72.0
MS.gene021927.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.39e-13 72.0
MS.gene021927.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.71e-13 71.6

Find 61 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAATCACCCTTCATTTAT+TGG 0.099357 7.1:-81421222 MS.gene021927:CDS
TGTAACATATTGTTATTATT+TGG 0.154767 7.1:+81421249 None:intergenic
CTTCAAGTCAAAATTCTTCT+TGG 0.156785 7.1:-81421404 MS.gene021927:CDS
AGTGAACTCTTGTTCTTTAT+TGG 0.160335 7.1:+81420849 None:intergenic
AATTCTCTTGAAACTTCTTT+AGG 0.204373 7.1:+81421372 None:intergenic
GTTCCTACAGGTGGAAAATT+AGG 0.209351 7.1:+81421519 None:intergenic
CTTGATACTCTGACAATTCT+TGG 0.239929 7.1:+81421738 None:intergenic
GTACTATTCATCAAGTAATT+TGG 0.245121 7.1:+81421336 None:intergenic
TGCTGAAAATTCTTCTCAAT+TGG 0.271973 7.1:-81421142 MS.gene021927:CDS
ATTCTCTTGAAACTTCTTTA+GGG 0.288069 7.1:+81421373 None:intergenic
CCAATAAATGAAGGGTGATT+TGG 0.289695 7.1:+81421222 None:intergenic
ATTCTTGGATCTTTCAATCT+TGG 0.296306 7.1:+81421753 None:intergenic
GTCAGAGTATCAAGAGCTTT+TGG 0.318336 7.1:-81421729 MS.gene021927:CDS
TAAAGAAGTTTCAAGAGAAT+TGG 0.320601 7.1:-81421370 MS.gene021927:CDS
AATTCTTCTCAAATGATGTT+AGG 0.334825 7.1:-81421066 MS.gene021927:CDS
TCAATAGGAACAATATTCAT+TGG 0.339202 7.1:-81421437 MS.gene021927:CDS
TATGATCTTCAAGATAGGTT+AGG 0.344793 7.1:-81421618 MS.gene021927:CDS
GATTGAGAGATAGAAGAATA+AGG 0.365377 7.1:-81421668 MS.gene021927:CDS
CCAATGTTGTCTTTATCAAC+TGG 0.374206 7.1:-81421090 MS.gene021927:CDS
TGATGAACCTCTTGTTTCAA+TGG 0.375029 7.1:+81421846 None:intergenic
AACAATTGTTCAATTTCAAT+AGG 0.377158 7.1:-81421452 MS.gene021927:CDS
AACCTAGCAAAGTTGTTGAT+TGG 0.387978 7.1:-81421587 MS.gene021927:CDS
GCTGAAAATTCTTCTCAATT+GGG 0.390841 7.1:-81421141 MS.gene021927:CDS
CAACAATCCATCCTTCAGTT+TGG 0.407175 7.1:-81420905 MS.gene021927:CDS
GTAGGAACAAATTTCAACTT+TGG 0.419771 7.1:-81421504 MS.gene021927:CDS
AACTTTATGATCTTCAAGAT+AGG 0.421843 7.1:-81421623 MS.gene021927:CDS
ATGATCTTCAAGATAGGTTA+GGG 0.433727 7.1:-81421617 MS.gene021927:CDS
AGCAAAGTATGCACAATAAG+AGG 0.437085 7.1:-81421690 MS.gene021927:CDS
TTTAATAAACCAATAAATGA+AGG 0.455410 7.1:+81421213 None:intergenic
CAATAGGAACAATATTCATT+GGG 0.458388 7.1:-81421436 MS.gene021927:CDS
TTGAAATTTGTTCCTACAGG+TGG 0.465228 7.1:+81421510 None:intergenic
TTGTTGAGAGAATTAGTCAT+TGG 0.470696 7.1:+81420922 None:intergenic
AAGTTGAAATTTGTTCCTAC+AGG 0.474911 7.1:+81421507 None:intergenic
TTTGATGATAATGATGGTTG+TGG 0.483862 7.1:+81420974 None:intergenic
ACTTGGTACTAATTACCAAT+GGG 0.492610 7.1:-81421181 MS.gene021927:CDS
TTCTTCAAATGTTAATGCAA+TGG 0.492790 7.1:-81421025 MS.gene021927:CDS
TAGAAATTTGGCCAAACTGA+AGG 0.496121 7.1:+81420894 None:intergenic
TTGTTGAGTTCATTAGAAGA+TGG 0.499012 7.1:+81421477 None:intergenic
TCTTCTCAATTGGGAAACAA+TGG 0.501500 7.1:-81421132 MS.gene021927:CDS
TTATTAAATCCTATGCCACT+TGG 0.521662 7.1:-81421198 MS.gene021927:CDS
AAATGTTAATGCAATGGAGA+TGG 0.523566 7.1:-81421019 MS.gene021927:CDS
ACAGGTGGAAAATTAGGTAA+TGG 0.536982 7.1:+81421525 None:intergenic
CCAGTTGATAAAGACAACAT+TGG 0.545201 7.1:+81421090 None:intergenic
TCAACATTTGATGATAATGA+TGG 0.545561 7.1:+81420968 None:intergenic
AACCAATCAACAACTTTGCT+AGG 0.547433 7.1:+81421585 None:intergenic
TAATTAGTACCAAGTGGCAT+AGG 0.547722 7.1:+81421189 None:intergenic
GATGAAAATATTAACAAGCA+AGG 0.549563 7.1:-81421306 MS.gene021927:CDS
CACTTGGTACTAATTACCAA+TGG 0.556420 7.1:-81421182 MS.gene021927:CDS
CATTGGAAGTACCTCCCCAT+TGG 0.558728 7.1:+81421166 None:intergenic
AATTTGGCCAAACTGAAGGA+TGG 0.562622 7.1:+81420898 None:intergenic
TGGCCATTGTGATGAAGCTA+AGG 0.563573 7.1:+81421773 None:intergenic
AGGAACAATATTCATTGGGA+AGG 0.563907 7.1:-81421432 MS.gene021927:CDS
GGTGGAAAATTAGGTAATGG+TGG 0.564196 7.1:+81421528 None:intergenic
GTTGATAAAGACAACATTGG+TGG 0.570845 7.1:+81421093 None:intergenic
TCATAAAGTTGAATAGCTGT+TGG 0.592296 7.1:+81421636 None:intergenic
TTAATAAACCAATAAATGAA+GGG 0.612685 7.1:+81421214 None:intergenic
GAAGCTAAGGAGCTTGATGA+TGG 0.616225 7.1:+81421786 None:intergenic
GCTCCTTAGCTTCATCACAA+TGG 0.647279 7.1:-81421776 MS.gene021927:CDS
GGTACTAATTACCAATGGGG+AGG 0.679474 7.1:-81421177 MS.gene021927:CDS
CATTGGTAATTAGTACCAAG+TGG 0.701178 7.1:+81421183 None:intergenic
CTTGGTACTAATTACCAATG+GGG 0.737610 7.1:-81421180 MS.gene021927:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAAACCAATAAATGAA+GGG + chr7.1:81421502-81421521 None:intergenic 15.0%
!! TTTAATAAACCAATAAATGA+AGG + chr7.1:81421503-81421522 None:intergenic 15.0%
!!! TGTAACATATTGTTATTATT+TGG + chr7.1:81421467-81421486 None:intergenic 15.0%
!! AACAATTGTTCAATTTCAAT+AGG - chr7.1:81421261-81421280 MS.gene021927:CDS 20.0%
! AAACGATCATGAAAAAGAAA+AGG - chr7.1:81420896-81420915 MS.gene021927:CDS 25.0%
! AACTTTATGATCTTCAAGAT+AGG - chr7.1:81421090-81421109 MS.gene021927:CDS 25.0%
! AATTCTCTTGAAACTTCTTT+AGG + chr7.1:81421344-81421363 None:intergenic 25.0%
! AATTCTTCTCAAATGATGTT+AGG - chr7.1:81421647-81421666 MS.gene021927:CDS 25.0%
! ATTCTCTTGAAACTTCTTTA+GGG + chr7.1:81421343-81421362 None:intergenic 25.0%
! CAATAGGAACAATATTCATT+GGG - chr7.1:81421277-81421296 MS.gene021927:CDS 25.0%
! GATGAAAATATTAACAAGCA+AGG - chr7.1:81421407-81421426 MS.gene021927:CDS 25.0%
! GTACTATTCATCAAGTAATT+TGG + chr7.1:81421380-81421399 None:intergenic 25.0%
! TAAAGAAGTTTCAAGAGAAT+TGG - chr7.1:81421343-81421362 MS.gene021927:CDS 25.0%
! TCAATAGGAACAATATTCAT+TGG - chr7.1:81421276-81421295 MS.gene021927:CDS 25.0%
! TTCTTCAAATGTTAATGCAA+TGG - chr7.1:81421688-81421707 MS.gene021927:CDS 25.0%
!! TCAACATTTGATGATAATGA+TGG + chr7.1:81421748-81421767 None:intergenic 25.0%
AAATGTTAATGCAATGGAGA+TGG - chr7.1:81421694-81421713 MS.gene021927:CDS 30.0%
ACTTGGTACTAATTACCAAT+GGG - chr7.1:81421532-81421551 MS.gene021927:CDS 30.0%
AGTGAACTCTTGTTCTTTAT+TGG + chr7.1:81421867-81421886 None:intergenic 30.0%
ATGATCTTCAAGATAGGTTA+GGG - chr7.1:81421096-81421115 MS.gene021927:CDS 30.0%
ATTCTTGGATCTTTCAATCT+TGG + chr7.1:81420963-81420982 None:intergenic 30.0%
CTTCAAGTCAAAATTCTTCT+TGG - chr7.1:81421309-81421328 MS.gene021927:CDS 30.0%
GATTGAGAGATAGAAGAATA+AGG - chr7.1:81421045-81421064 MS.gene021927:CDS 30.0%
GCTGAAAATTCTTCTCAATT+GGG - chr7.1:81421572-81421591 MS.gene021927:CDS 30.0%
GTAGGAACAAATTTCAACTT+TGG - chr7.1:81421209-81421228 MS.gene021927:CDS 30.0%
TATGATCTTCAAGATAGGTT+AGG - chr7.1:81421095-81421114 MS.gene021927:CDS 30.0%
TCATAAAGTTGAATAGCTGT+TGG + chr7.1:81421080-81421099 None:intergenic 30.0%
TGCTGAAAATTCTTCTCAAT+TGG - chr7.1:81421571-81421590 MS.gene021927:CDS 30.0%
TTATTAAATCCTATGCCACT+TGG - chr7.1:81421515-81421534 MS.gene021927:CDS 30.0%
TTGTTGAGAGAATTAGTCAT+TGG + chr7.1:81421794-81421813 None:intergenic 30.0%
TTGTTGAGTTCATTAGAAGA+TGG + chr7.1:81421239-81421258 None:intergenic 30.0%
TTTGGAAAAACATTTGCAGT+AGG + chr7.1:81421449-81421468 None:intergenic 30.0%
! AAGTTGAAATTTGTTCCTAC+AGG + chr7.1:81421209-81421228 None:intergenic 30.0%
! AGAAGAATTTTCAGCATCAT+TGG + chr7.1:81421567-81421586 None:intergenic 30.0%
! AGATTTTCCATTGAAACAAG+AGG - chr7.1:81420860-81420879 MS.gene021927:CDS 30.0%
! TTTGATGATAATGATGGTTG+TGG + chr7.1:81421742-81421761 None:intergenic 30.0%
!!! TCTGCTGTTTTGTAGAAATT+TGG + chr7.1:81421834-81421853 None:intergenic 30.0%
AACCAATCAACAACTTTGCT+AGG + chr7.1:81421131-81421150 None:intergenic 35.0%
AACCTAGCAAAGTTGTTGAT+TGG - chr7.1:81421126-81421145 MS.gene021927:CDS 35.0%
ACAGGTGGAAAATTAGGTAA+TGG + chr7.1:81421191-81421210 None:intergenic 35.0%
AGCAAAGTATGCACAATAAG+AGG - chr7.1:81421023-81421042 MS.gene021927:CDS 35.0%
AGGAACAATATTCATTGGGA+AGG - chr7.1:81421281-81421300 MS.gene021927:CDS 35.0%
CACTTGGTACTAATTACCAA+TGG - chr7.1:81421531-81421550 MS.gene021927:CDS 35.0%
CCAAATCACCCTTCATTTAT+TGG - chr7.1:81421491-81421510 MS.gene021927:CDS 35.0%
CCAATAAATGAAGGGTGATT+TGG + chr7.1:81421494-81421513 None:intergenic 35.0%
CCAATGTTGTCTTTATCAAC+TGG - chr7.1:81421623-81421642 MS.gene021927:CDS 35.0%
CTTGATACTCTGACAATTCT+TGG + chr7.1:81420978-81420997 None:intergenic 35.0%
CTTGGTACTAATTACCAATG+GGG - chr7.1:81421533-81421552 MS.gene021927:CDS 35.0%
TAATTAGTACCAAGTGGCAT+AGG + chr7.1:81421527-81421546 None:intergenic 35.0%
TAGAAATTTGGCCAAACTGA+AGG + chr7.1:81421822-81421841 None:intergenic 35.0%
TCTTCTCAATTGGGAAACAA+TGG - chr7.1:81421581-81421600 MS.gene021927:CDS 35.0%
! CATTGGTAATTAGTACCAAG+TGG + chr7.1:81421533-81421552 None:intergenic 35.0%
! GTTGATAAAGACAACATTGG+TGG + chr7.1:81421623-81421642 None:intergenic 35.0%
! TGATGAACCTCTTGTTTCAA+TGG + chr7.1:81420870-81420889 None:intergenic 35.0%
! TTACCTAATTTTCCACCTGT+AGG - chr7.1:81421191-81421210 MS.gene021927:CDS 35.0%
! TTGAAATTTGTTCCTACAGG+TGG + chr7.1:81421206-81421225 None:intergenic 35.0%
!! CCAGTTGATAAAGACAACAT+TGG + chr7.1:81421626-81421645 None:intergenic 35.0%
AATTTGGCCAAACTGAAGGA+TGG + chr7.1:81421818-81421837 None:intergenic 40.0%
CAACAATCCATCCTTCAGTT+TGG - chr7.1:81421808-81421827 MS.gene021927:CDS 40.0%
GTTCCTACAGGTGGAAAATT+AGG + chr7.1:81421197-81421216 None:intergenic 40.0%
! GGTGGAAAATTAGGTAATGG+TGG + chr7.1:81421188-81421207 None:intergenic 40.0%
!! AGAGTATCAAGAGCTTTTGG+TGG - chr7.1:81420987-81421006 MS.gene021927:CDS 40.0%
!! GTCAGAGTATCAAGAGCTTT+TGG - chr7.1:81420984-81421003 MS.gene021927:CDS 40.0%
GAAGCTAAGGAGCTTGATGA+TGG + chr7.1:81420930-81420949 None:intergenic 45.0%
GCTCCTTAGCTTCATCACAA+TGG - chr7.1:81420937-81420956 MS.gene021927:CDS 45.0%
GGTACTAATTACCAATGGGG+AGG - chr7.1:81421536-81421555 MS.gene021927:CDS 45.0%
TGGCCATTGTGATGAAGCTA+AGG + chr7.1:81420943-81420962 None:intergenic 45.0%
!! CATTGGAAGTACCTCCCCAT+TGG + chr7.1:81421550-81421569 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 81420840 81421895 81420840 ID=MS.gene021927
chr7.1 mRNA 81420840 81421895 81420840 ID=MS.gene021927.t1;Parent=MS.gene021927
chr7.1 exon 81420840 81421895 81420840 ID=MS.gene021927.t1.exon1;Parent=MS.gene021927.t1
chr7.1 CDS 81420840 81421895 81420840 ID=cds.MS.gene021927.t1;Parent=MS.gene021927.t1
Gene Sequence

>MS.gene021927

ATGAATAAGAATTCAAAAGAAGATTTTCCATTGAAACAAGAGGTTCATCAAAATTCAAACGATCATGAAAAAGAAAAGGCTAGTTCACCATCATCAAGCTCCTTAGCTTCATCACAATGGCCAAGATTGAAAGATCCAAGAATTGTCAGAGTATCAAGAGCTTTTGGTGGAAAAGATAGACATAGCAAAGTATGCACAATAAGAGGATTGAGAGATAGAAGAATAAGGCTTTCAGTACCAACAGCTATTCAACTTTATGATCTTCAAGATAGGTTAGGGCTTAATCAACCTAGCAAAGTTGTTGATTGGTTGCTCAATGCAGCTAAACATGACATTGATCAACTTCCACCATTACCTAATTTTCCACCTGTAGGAACAAATTTCAACTTTGGTTTTCCATCTTCTAATGAACTCAACAATCAACAATTGTTCAATTTCAATAGGAACAATATTCATTGGGAAGGTTCAACTTCAAGTCAAAATTCTTCTTGGAAATCAAACCCTAAAGAAGTTTCAAGAGAATTGGTTAATGATAAACCAAATTACTTGATGAATAGTACTCAAGAAGATGAAAATATTAACAAGCAAGGAAGCAATATTGAAGTTCCTACTGCAAATGTTTTTCCAAATAATAACAATATGTTACAAAGACCAAATCACCCTTCATTTATTGGTTTATTAAATCCTATGCCACTTGGTACTAATTACCAATGGGGAGGTACTTCCAATGATGCTGAAAATTCTTCTCAATTGGGAAACAATGGCTTTATTACATCTTTGCCACCAATGTTGTCTTTATCAACTGGAAATTCTTCTCAAATGATGTTAGGAACAACACAATCATATTTTTCTTCAAATGTTAATGCAATGGAGATGGATCATCATCATCAAAGAGAAATCCACAACCATCATTATCATCAAATGTTGAGTTCATCAAGTTCTTCTCAGAATCCAATGACTAATTCTCTCAACAATCCATCCTTCAGTTTGGCCAAATTTCTACAAAACAGCAGAGAAAATTCATCCAATAAAGAACAAGAGTTCACTACAAAGTGA

Protein sequence

>MS.gene021927.t1

MNKNSKEDFPLKQEVHQNSNDHEKEKASSPSSSSLASSQWPRLKDPRIVRVSRAFGGKDRHSKVCTIRGLRDRRIRLSVPTAIQLYDLQDRLGLNQPSKVVDWLLNAAKHDIDQLPPLPNFPPVGTNFNFGFPSSNELNNQQLFNFNRNNIHWEGSTSSQNSSWKSNPKEVSRELVNDKPNYLMNSTQEDENINKQGSNIEVPTANVFPNNNNMLQRPNHPSFIGLLNPMPLGTNYQWGGTSNDAENSSQLGNNGFITSLPPMLSLSTGNSSQMMLGTTQSYFSSNVNAMEMDHHHQREIHNHHYHQMLSSSSSSQNPMTNSLNNPSFSLAKFLQNSRENSSNKEQEFTTK