AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022125

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022125.t1 MTR_6g086300 77.311 238 44 2 193 420 212 449 1.09e-121 363
MS.gene022125.t1 MTR_6g086300 77.311 238 44 2 193 420 212 449 1.09e-121 363
MS.gene022125.t1 MTR_8g083530 69.328 238 63 2 193 420 213 450 2.43e-109 331
MS.gene022125.t1 MTR_6g086340 67.932 237 66 2 193 419 210 446 9.81e-99 304
MS.gene022125.t1 MTR_8g083520 61.345 238 49 3 193 420 124 328 7.02e-91 280
MS.gene022125.t1 MTR_8g083520 61.603 237 48 3 193 419 204 407 1.85e-89 279
MS.gene022125.t1 MTR_6g453220 82.209 163 28 1 1 162 310 472 1.72e-82 262
MS.gene022125.t1 MTR_8g064170 46.414 237 117 2 193 419 219 455 3.26e-71 233
MS.gene022125.t1 MTR_8g064170 46.414 237 117 2 193 419 251 487 6.74e-71 233
MS.gene022125.t1 MTR_3g078170 49.378 241 104 4 193 419 252 488 5.07e-69 228
MS.gene022125.t1 MTR_4g107040 46.218 238 118 2 193 420 254 491 2.33e-67 223
MS.gene022125.t1 MTR_3g078170 49.761 209 87 4 193 387 252 456 3.74e-59 200
MS.gene022125.t1 MTR_8g083530 67.910 134 33 2 193 316 213 346 1.09e-52 181
MS.gene022125.t1 MTR_3g078170 49.733 187 76 4 193 365 252 434 2.24e-49 174
MS.gene022125.t1 MTR_3g078170 50.000 184 74 4 193 362 252 431 3.93e-49 172
MS.gene022125.t1 MTR_3g078550 36.134 238 139 4 194 419 175 411 1.57e-43 158
MS.gene022125.t1 MTR_3g078550 36.134 238 139 4 194 419 205 441 2.52e-43 159
MS.gene022125.t1 MTR_4g106510 35.714 238 140 4 194 419 205 441 3.64e-43 158
MS.gene022125.t1 MTR_3g078490 35.146 239 142 4 194 420 235 472 6.84e-42 155
MS.gene022125.t1 MTR_2g067660 37.946 224 132 3 191 408 237 459 1.53e-40 151
MS.gene022125.t1 MTR_1g013170 64.486 107 38 0 48 154 186 292 7.77e-30 121
MS.gene022125.t1 MTR_1690s0010 37.500 176 100 3 169 335 176 350 1.40e-29 118
MS.gene022125.t1 MTR_2g034160 28.226 248 166 6 183 420 237 482 9.83e-24 103
MS.gene022125.t1 MTR_3g106220 64.865 74 25 1 70 143 215 287 2.27e-22 100
MS.gene022125.t1 MTR_7g117705 41.781 146 38 4 70 174 208 347 3.29e-19 89.7
MS.gene022125.t1 MTR_3g078490 39.394 132 69 3 194 315 205 335 3.66e-19 89.0
MS.gene022125.t1 MTR_1399s0020 29.167 168 116 1 253 420 129 293 1.27e-15 78.2
MS.gene022125.t1 MTR_1g014320 24.576 236 164 4 193 420 211 440 1.73e-15 78.6
MS.gene022125.t1 MTR_2g009750 30.131 229 143 7 193 407 203 428 3.04e-15 77.8
MS.gene022125.t1 MTR_4g093170 29.515 227 143 7 193 405 230 453 4.40e-15 77.4
MS.gene022125.t1 MTR_2g009750 29.825 228 144 7 193 407 202 426 8.50e-15 76.6
MS.gene022125.t1 MTR_2g009750 29.825 228 144 7 193 407 203 427 8.83e-15 76.6
MS.gene022125.t1 MTR_1g013180 40.876 137 61 5 51 179 340 464 2.67e-14 75.5
MS.gene022125.t1 MTR_7g098550 57.778 90 34 2 1 86 282 371 6.09e-14 73.9
MS.gene022125.t1 MTR_7g098550 57.778 90 34 2 1 86 290 379 6.42e-14 73.9
MS.gene022125.t1 MTR_4g131180 24.473 237 163 6 193 420 211 440 7.73e-14 73.6
MS.gene022125.t1 MTR_4g131180 24.473 237 163 6 193 420 211 440 8.21e-14 73.6
MS.gene022125.t1 MTR_2g042330 26.531 196 142 2 215 409 241 435 1.02e-13 73.2
MS.gene022125.t1 MTR_1314s0010 27.174 184 117 4 217 389 83 260 1.32e-13 72.0
MS.gene022125.t1 MTR_2g034960 50.000 64 32 0 70 133 228 291 2.13e-13 72.4
MS.gene022125.t1 MTR_7g098550 77.966 59 12 1 1 58 290 348 2.28e-13 71.6
MS.gene022125.t1 MTR_1g032570 58.929 56 23 0 70 125 148 203 3.56e-13 71.6
MS.gene022125.t1 MTR_4g121020 50.704 71 32 1 63 133 121 188 3.79e-13 71.6
MS.gene022125.t1 MTR_1g032570 50.000 68 34 0 70 137 217 284 4.06e-13 71.6
MS.gene022125.t1 MTR_4g121020 50.704 71 32 1 63 133 225 292 4.84e-13 71.2
MS.gene022125.t1 MTR_4g121020 50.704 71 32 1 63 133 225 292 4.85e-13 71.2
MS.gene022125.t1 MTR_3g102600 44.000 75 34 1 70 144 100 166 2.75e-12 65.5
MS.gene022125.t1 MTR_3g102600 44.000 75 34 1 70 144 149 215 4.58e-12 65.9
MS.gene022125.t1 MTR_3g102590 64.706 51 16 1 70 118 160 210 1.27e-11 64.7
MS.gene022125.t1 MTR_2g450070 54.795 73 28 3 70 141 230 298 1.37e-11 67.0
MS.gene022125.t1 MTR_2g450070 54.795 73 28 3 70 141 230 298 1.50e-11 66.6
MS.gene022125.t1 MTR_2g072400 41.558 77 40 3 14 86 311 386 4.76e-11 65.1
MS.gene022125.t1 MTR_1g085680 48.276 58 29 1 10 66 307 364 6.10e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022125.t1 AT3G24503 62.447 237 79 2 193 419 211 447 7.04e-96 296
MS.gene022125.t1 AT3G48000 48.739 238 112 2 193 420 248 485 1.63e-71 234
MS.gene022125.t1 AT1G23800 49.174 242 105 4 193 420 244 481 3.30e-70 231
MS.gene022125.t1 AT3G48170 36.134 238 139 4 194 419 205 441 5.85e-44 160
MS.gene022125.t1 AT1G74920 35.983 239 140 4 194 420 200 437 8.97e-44 159
MS.gene022125.t1 AT1G74920 35.983 239 140 4 194 420 205 442 1.33e-43 159
MS.gene022125.t1 AT1G79440 36.000 225 133 3 194 408 242 465 1.77e-35 137
MS.gene022125.t1 AT1G23800 50.000 138 51 4 193 316 244 377 4.70e-32 126
MS.gene022125.t1 AT3G66658 28.151 238 159 6 194 420 246 482 4.09e-22 99.4
MS.gene022125.t1 AT3G66658 28.151 238 159 6 194 420 246 482 5.82e-22 98.6
MS.gene022125.t1 AT1G54100 30.653 199 126 5 215 407 240 432 3.76e-19 90.1
MS.gene022125.t1 AT1G54100 30.653 199 126 5 215 407 240 432 3.76e-19 90.1
MS.gene022125.t1 AT2G14170 31.858 226 139 5 193 405 302 525 2.79e-17 84.3
MS.gene022125.t1 AT2G14170 31.858 226 139 5 193 405 302 525 3.26e-17 84.3
MS.gene022125.t1 AT2G14170 31.858 226 139 5 193 405 193 416 5.73e-17 83.2
MS.gene022125.t1 AT3G16857 49.383 81 35 1 70 150 184 258 2.65e-15 78.6
MS.gene022125.t1 AT3G16857 49.383 81 35 1 70 150 254 328 3.06e-15 78.2
MS.gene022125.t1 AT3G16857 49.383 81 35 1 70 150 254 328 3.69e-15 78.2
MS.gene022125.t1 AT5G58080 54.545 66 28 1 70 135 211 274 1.77e-13 72.8
MS.gene022125.t1 AT2G25180 49.333 75 32 2 70 143 212 281 2.85e-13 72.0
MS.gene022125.t1 AT2G24270 24.898 245 152 8 193 420 251 480 2.99e-13 72.0
MS.gene022125.t1 AT2G24270 24.898 245 152 8 193 420 211 440 3.68e-13 71.6
MS.gene022125.t1 AT2G24270 24.898 245 152 8 193 420 211 440 3.83e-13 71.6
MS.gene022125.t1 AT2G24270 24.898 245 152 8 193 420 211 440 3.83e-13 71.6
MS.gene022125.t1 AT1G75130 55.932 59 25 1 1 58 288 346 6.03e-13 70.9
MS.gene022125.t1 AT2G46950 56.000 50 21 1 10 58 360 409 1.07e-11 67.0
MS.gene022125.t1 AT2G46950 56.000 50 21 1 10 58 360 409 1.10e-11 66.6
MS.gene022125.t1 AT2G01760 35.338 133 70 5 70 196 152 274 4.05e-11 64.3
MS.gene022125.t1 AT2G01760 35.338 133 70 5 70 196 217 339 5.49e-11 64.3
MS.gene022125.t1 AT2G01760 35.338 133 70 5 70 196 245 367 6.57e-11 64.3
MS.gene022125.t1 AT2G46960 51.852 54 25 1 6 58 186 239 7.89e-11 63.9

Find 78 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGTAAGGTTAGCTTTAC+TGG 0.225096 7.4:+80237844 MS.gene022125:CDS
AGTAATTGTGACAAAGAATT+TGG 0.234315 7.4:+80238413 MS.gene022125:CDS
CGAGGACCTTGTTGAACTTT+AGG 0.236292 7.4:-80238135 None:intergenic
AAACCAGCTTCACTAGAATT+AGG 0.247929 7.4:+80237913 MS.gene022125:CDS
TTAGATAGAGTTTATACTTC+TGG 0.249305 7.4:-80237513 None:intergenic
TATTAACCATGTTGATATTA+AGG 0.251515 7.4:+80237741 MS.gene022125:CDS
ACAGAAGGTGCAATGGTATT+TGG 0.304024 7.4:+80237668 MS.gene022125:CDS
ACAACACAAGATGAGATATT+TGG 0.306145 7.4:+80238309 MS.gene022125:CDS
CAACATCTAACTTGTTAACT+TGG 0.322163 7.4:+80237305 MS.gene022125:CDS
CTCATGAAGTTCAAGACTAT+TGG 0.328116 7.4:+80238345 MS.gene022125:CDS
TTCAACAAGACGTGAAATAA+TGG 0.361036 7.4:+80237867 MS.gene022125:CDS
TCCAACTCCACTTCTTAAAT+AGG 0.361147 7.4:-80237605 None:intergenic
TTAACATTGGAGAAAATTGT+AGG 0.377777 7.4:-80238273 None:intergenic
GAATGTAAAACCTACTTTGC+TGG 0.377877 7.4:+80237274 MS.gene022125:CDS
GGAAGATAAGTGTTTGTTCC+AGG 0.388463 7.4:-80237709 None:intergenic
AGTGCCAACAATAAAAGATA+TGG 0.406439 7.4:+80238381 MS.gene022125:CDS
CCTCCTAATTCTAGTGAAGC+TGG 0.408286 7.4:-80237916 None:intergenic
CTCGATGTAGTACCTGGATT+TGG 0.433604 7.4:+80237808 MS.gene022125:CDS
TTTATTGTTGGCACTTCTGA+TGG 0.435959 7.4:-80238373 None:intergenic
GGAAAACAAACAAGATCATA+TGG 0.443079 7.4:+80237552 MS.gene022125:CDS
TGCATCTGAACTACTAGTTA+AGG 0.451210 7.4:-80237581 None:intergenic
TGTTGAACTTTAGGATCAAA+AGG 0.452062 7.4:-80238126 None:intergenic
TGAATTTGAGAAGATGTTAG+TGG 0.466452 7.4:+80238074 MS.gene022125:CDS
AACTTCATGAGTGCCATGAC+AGG 0.481827 7.4:-80238333 None:intergenic
CTAAAGTTCAACAAGGTCCT+CGG 0.483519 7.4:+80238136 MS.gene022125:CDS
CTCATCTTGTGTTGTATGCA+TGG 0.484702 7.4:-80238301 None:intergenic
ATTCTTTCATATATTGAGCA+TGG 0.484714 7.4:+80238183 MS.gene022125:CDS
ACATGGTTAATAGATTCTCT+TGG 0.487239 7.4:-80237730 None:intergenic
AACAATAAAAGATATGGCTT+AGG 0.489878 7.4:+80238387 MS.gene022125:CDS
ATCTGAAATTCCAGCCGGTA+TGG 0.495993 7.4:-80237779 None:intergenic
CATTAGCATCGTATTTAACA+AGG 0.497087 7.4:+80237999 MS.gene022125:CDS
TCCTATTTAAGAAGTGGAGT+TGG 0.497128 7.4:+80237604 MS.gene022125:CDS
GATGCATCCTATTTAAGAAG+TGG 0.500111 7.4:+80237598 MS.gene022125:CDS
AAGACACGTGAAGAAATTGA+TGG 0.504719 7.4:+80237367 MS.gene022125:CDS
TCTCGTGTGTTTGTTCAAGA+AGG 0.505481 7.4:+80238042 MS.gene022125:CDS
AAAGCAAGAGCTTGGGCTGT+TGG 0.506234 7.4:+80238099 MS.gene022125:CDS
GGAGTACTCGATGTAGTACC+TGG 0.509801 7.4:+80237802 MS.gene022125:CDS
AACATCTAACTTGTTAACTT+GGG 0.513062 7.4:+80237306 MS.gene022125:CDS
CCTGCAGTTGCACCAAATCC+AGG 0.513945 7.4:-80237820 None:intergenic
ATTGAGCATGGACAGAAAGA+TGG 0.515764 7.4:+80238195 MS.gene022125:CDS
TATTAGCAAAATATCAAGAA+TGG 0.516199 7.4:+80237338 MS.gene022125:CDS
AGTTTCTACAAGAATGAAGT+TGG 0.522151 7.4:+80237214 MS.gene022125:CDS
TAAAGTTCAACAAGGTCCTC+GGG 0.523355 7.4:+80238137 MS.gene022125:CDS
TTTGGTGCAACTGCAGGAAC+TGG 0.523481 7.4:+80237826 MS.gene022125:CDS
TGGAGAAGATAGACTACAGC+TGG 0.525660 7.4:+80237237 MS.gene022125:CDS
TATTTAAGAAGTGGAGTTGG+AGG 0.527033 7.4:+80237607 MS.gene022125:CDS
CCTGGATTTGGTGCAACTGC+AGG 0.534107 7.4:+80237820 MS.gene022125:CDS
AGTGGACAAAGCAAGAGCTT+GGG 0.542455 7.4:+80238092 MS.gene022125:CDS
TTTATACTTCTGGAGACGGC+TGG 0.543747 7.4:-80237503 None:intergenic
TAGTGGACAAAGCAAGAGCT+TGG 0.545318 7.4:+80238091 MS.gene022125:CDS
ATACTTTACTTGAAAACAGA+AGG 0.547861 7.4:+80237653 MS.gene022125:CDS
TCAAGCACAAAGAAACTGCC+TGG 0.551817 7.4:+80237691 MS.gene022125:CDS
CCAAATAACTCATGTTCGAA+TGG 0.554923 7.4:-80238460 None:intergenic
TTCTACAAGAATGAAGTTGG+TGG 0.559129 7.4:+80237217 MS.gene022125:CDS
CTTGAAAACAGAAGGTGCAA+TGG 0.560167 7.4:+80237661 MS.gene022125:CDS
ATGCATGGCCTCCTTAACAT+TGG 0.566139 7.4:-80238286 None:intergenic
TGAGATATTTGGCCCTGTCA+TGG 0.568944 7.4:+80238320 MS.gene022125:CDS
TCGTTAAAGACCATACCGGC+TGG 0.570747 7.4:+80237769 MS.gene022125:CDS
AGAGTTTATACTTCTGGAGA+CGG 0.571940 7.4:-80237507 None:intergenic
GATGGAGTAACTCTTTCGAC+AGG 0.572416 7.4:+80238213 MS.gene022125:CDS
TGCAACTGCAGGAACTGGTA+AGG 0.578355 7.4:+80237831 MS.gene022125:CDS
TGAAAAGCTGACATGGGAGA+AGG 0.578825 7.4:+80237477 MS.gene022125:CDS
ATATAGCAAGCATCGTATCA+AGG 0.582360 7.4:+80238436 MS.gene022125:CDS
AAGGTCCTCGGGCTGACAAG+AGG 0.587054 7.4:+80238148 MS.gene022125:CDS
ATACCGGCTGGAATTTCAGA+TGG 0.591251 7.4:+80237781 MS.gene022125:CDS
CCAGCTTCACTAGAATTAGG+AGG 0.605466 7.4:+80237916 MS.gene022125:CDS
GAGTTCGTTAAAGACCATAC+CGG 0.606961 7.4:+80237765 MS.gene022125:CDS
AGACACGTGAAGAAATTGAT+GGG 0.609154 7.4:+80237368 MS.gene022125:CDS
TGAATGTTGAAAAGCTGACA+TGG 0.624466 7.4:+80237470 MS.gene022125:CDS
AATTTCCTCTTGTCAGCCCG+AGG 0.625717 7.4:-80238153 None:intergenic
ACTTCATGAGTGCCATGACA+GGG 0.635798 7.4:-80238332 None:intergenic
ACTCCATCTGAAATTCCAGC+CGG 0.636139 7.4:-80237784 None:intergenic
AACTCTCCTTAATATCAACA+TGG 0.638325 7.4:-80237747 None:intergenic
ATTAGCATCGTATTTAACAA+GGG 0.639693 7.4:+80238000 MS.gene022125:CDS
AAAGCTGACATGGGAGAAGG+CGG 0.658945 7.4:+80237480 MS.gene022125:CDS
TTTGATCCTAAAGTTCAACA+AGG 0.673250 7.4:+80238129 MS.gene022125:CDS
TAATCAATTAGGCATTGACA+AGG 0.692865 7.4:+80237420 MS.gene022125:CDS
GAATGTTGAAAAGCTGACAT+GGG 0.714309 7.4:+80237471 MS.gene022125:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCATTATAAAAAATTAAGA+GGG - chr7.4:80237950-80237969 None:intergenic 15.0%
!! CAGCATTATAAAAAATTAAG+AGG - chr7.4:80237951-80237970 None:intergenic 20.0%
!! GCATTATAAAAAATTAAGAG+GGG - chr7.4:80237949-80237968 None:intergenic 20.0%
!! TAGAGAAGAAATTGAAAAAA+GGG + chr7.4:80237396-80237415 MS.gene022125:CDS 20.0%
!! TATTAACCATGTTGATATTA+AGG + chr7.4:80237741-80237760 MS.gene022125:CDS 20.0%
!! TATTAGCAAAATATCAAGAA+TGG + chr7.4:80237338-80237357 MS.gene022125:CDS 20.0%
!! TCTAAAAAAAGTTAATTGTG+TGG + chr7.4:80237531-80237550 MS.gene022125:CDS 20.0%
! AACAATAAAAGATATGGCTT+AGG + chr7.4:80238387-80238406 MS.gene022125:CDS 25.0%
! AGTAATTGTGACAAAGAATT+TGG + chr7.4:80238413-80238432 MS.gene022125:CDS 25.0%
! ATACTTTACTTGAAAACAGA+AGG + chr7.4:80237653-80237672 MS.gene022125:CDS 25.0%
! ATTAGCATCGTATTTAACAA+GGG + chr7.4:80238000-80238019 MS.gene022125:CDS 25.0%
! ATTCTTTCATATATTGAGCA+TGG + chr7.4:80238183-80238202 MS.gene022125:CDS 25.0%
! CTAGAGAAGAAATTGAAAAA+AGG + chr7.4:80237395-80237414 MS.gene022125:CDS 25.0%
! GAAAAAAGGGTTAATCAATT+AGG + chr7.4:80237409-80237428 MS.gene022125:CDS 25.0%
! TTAACATTGGAGAAAATTGT+AGG - chr7.4:80238276-80238295 None:intergenic 25.0%
! TTAGATAGAGTTTATACTTC+TGG - chr7.4:80237516-80237535 None:intergenic 25.0%
!! ATCAGTTCAAGAATCTTTTT+CGG - chr7.4:80237451-80237470 None:intergenic 25.0%
!! TAAGCCATATCTTTTATTGT+TGG - chr7.4:80238388-80238407 None:intergenic 25.0%
!! TACAATTTTCTCCAATGTTA+AGG + chr7.4:80238275-80238294 MS.gene022125:CDS 25.0%
!!! AACATCTAACTTGTTAACTT+GGG + chr7.4:80237306-80237325 MS.gene022125:CDS 25.0%
AACTCTCCTTAATATCAACA+TGG - chr7.4:80237750-80237769 None:intergenic 30.0%
ACAACACAAGATGAGATATT+TGG + chr7.4:80238309-80238328 MS.gene022125:CDS 30.0%
ACATGGTTAATAGATTCTCT+TGG - chr7.4:80237733-80237752 None:intergenic 30.0%
AGTGCCAACAATAAAAGATA+TGG + chr7.4:80238381-80238400 MS.gene022125:CDS 30.0%
AGTTTCTACAAGAATGAAGT+TGG + chr7.4:80237214-80237233 MS.gene022125:CDS 30.0%
CATTAGCATCGTATTTAACA+AGG + chr7.4:80237999-80238018 MS.gene022125:CDS 30.0%
GGAAAACAAACAAGATCATA+TGG + chr7.4:80237552-80237571 MS.gene022125:CDS 30.0%
TCGACAGGTAGTAAAAAAAT+TGG + chr7.4:80238228-80238247 MS.gene022125:CDS 30.0%
TGAATTTGAGAAGATGTTAG+TGG + chr7.4:80238074-80238093 MS.gene022125:CDS 30.0%
TTCAACAAGACGTGAAATAA+TGG + chr7.4:80237867-80237886 MS.gene022125:CDS 30.0%
TTTGATCCTAAAGTTCAACA+AGG + chr7.4:80238129-80238148 MS.gene022125:CDS 30.0%
! AATTTTCTCCAATGTTAAGG+AGG + chr7.4:80238278-80238297 MS.gene022125:CDS 30.0%
!! CAACATCTAACTTGTTAACT+TGG + chr7.4:80237305-80237324 MS.gene022125:CDS 30.0%
!! TAATCAATTAGGCATTGACA+AGG + chr7.4:80237420-80237439 MS.gene022125:CDS 30.0%
!! TGTTGAACTTTAGGATCAAA+AGG - chr7.4:80238129-80238148 None:intergenic 30.0%
AAACCAGCTTCACTAGAATT+AGG + chr7.4:80237913-80237932 MS.gene022125:CDS 35.0%
AAGACACGTGAAGAAATTGA+TGG + chr7.4:80237367-80237386 MS.gene022125:CDS 35.0%
AGACACGTGAAGAAATTGAT+GGG + chr7.4:80237368-80237387 MS.gene022125:CDS 35.0%
CTCATGAAGTTCAAGACTAT+TGG + chr7.4:80238345-80238364 MS.gene022125:CDS 35.0%
GAATGTAAAACCTACTTTGC+TGG + chr7.4:80237274-80237293 MS.gene022125:CDS 35.0%
GAATGTTGAAAAGCTGACAT+GGG + chr7.4:80237471-80237490 MS.gene022125:CDS 35.0%
GATGCATCCTATTTAAGAAG+TGG + chr7.4:80237598-80237617 MS.gene022125:CDS 35.0%
TATTTAAGAAGTGGAGTTGG+AGG + chr7.4:80237607-80237626 MS.gene022125:CDS 35.0%
TCCAACTCCACTTCTTAAAT+AGG - chr7.4:80237608-80237627 None:intergenic 35.0%
TCCTATTTAAGAAGTGGAGT+TGG + chr7.4:80237604-80237623 MS.gene022125:CDS 35.0%
TGAATGTTGAAAAGCTGACA+TGG + chr7.4:80237470-80237489 MS.gene022125:CDS 35.0%
TGCATCTGAACTACTAGTTA+AGG - chr7.4:80237584-80237603 None:intergenic 35.0%
TTCTACAAGAATGAAGTTGG+TGG + chr7.4:80237217-80237236 MS.gene022125:CDS 35.0%
TTTATTGTTGGCACTTCTGA+TGG - chr7.4:80238376-80238395 None:intergenic 35.0%
! AGAGTTTATACTTCTGGAGA+CGG - chr7.4:80237510-80237529 None:intergenic 35.0%
! ATATAGCAAGCATCGTATCA+AGG + chr7.4:80238436-80238455 MS.gene022125:CDS 35.0%
! TGTATCTTTTCCAGCAAAGT+AGG - chr7.4:80237287-80237306 None:intergenic 35.0%
ATTGAGCATGGACAGAAAGA+TGG + chr7.4:80238195-80238214 MS.gene022125:CDS 40.0%
CTAAAGTTCAACAAGGTCCT+CGG + chr7.4:80238136-80238155 MS.gene022125:CDS 40.0%
CTCATCTTGTGTTGTATGCA+TGG - chr7.4:80238304-80238323 None:intergenic 40.0%
GAGTTCGTTAAAGACCATAC+CGG + chr7.4:80237765-80237784 MS.gene022125:CDS 40.0%
GGAAGATAAGTGTTTGTTCC+AGG - chr7.4:80237712-80237731 None:intergenic 40.0%
TAAAGTTCAACAAGGTCCTC+GGG + chr7.4:80238137-80238156 MS.gene022125:CDS 40.0%
TCTCGTGTGTTTGTTCAAGA+AGG + chr7.4:80238042-80238061 MS.gene022125:CDS 40.0%
! ACAGAAGGTGCAATGGTATT+TGG + chr7.4:80237668-80237687 MS.gene022125:CDS 40.0%
! CTTGAAAACAGAAGGTGCAA+TGG + chr7.4:80237661-80237680 MS.gene022125:CDS 40.0%
!! ACTGGTAAGGTTAGCTTTAC+TGG + chr7.4:80237844-80237863 MS.gene022125:CDS 40.0%
AACTTCATGAGTGCCATGAC+AGG - chr7.4:80238336-80238355 None:intergenic 45.0%
ACTTCATGAGTGCCATGACA+GGG - chr7.4:80238335-80238354 None:intergenic 45.0%
AGTGGACAAAGCAAGAGCTT+GGG + chr7.4:80238092-80238111 MS.gene022125:CDS 45.0%
ATGCATGGCCTCCTTAACAT+TGG - chr7.4:80238289-80238308 None:intergenic 45.0%
CCAGCTTCACTAGAATTAGG+AGG + chr7.4:80237916-80237935 MS.gene022125:CDS 45.0%
CCTCCTAATTCTAGTGAAGC+TGG - chr7.4:80237919-80237938 None:intergenic 45.0%
CGAGGACCTTGTTGAACTTT+AGG - chr7.4:80238138-80238157 None:intergenic 45.0%
CTCGATGTAGTACCTGGATT+TGG + chr7.4:80237808-80237827 MS.gene022125:CDS 45.0%
GATGGAGTAACTCTTTCGAC+AGG + chr7.4:80238213-80238232 MS.gene022125:CDS 45.0%
TCAAGCACAAAGAAACTGCC+TGG + chr7.4:80237691-80237710 MS.gene022125:CDS 45.0%
TGAAAAGCTGACATGGGAGA+AGG + chr7.4:80237477-80237496 MS.gene022125:CDS 45.0%
TGAGATATTTGGCCCTGTCA+TGG + chr7.4:80238320-80238339 MS.gene022125:CDS 45.0%
TGGAGAAGATAGACTACAGC+TGG + chr7.4:80237237-80237256 MS.gene022125:CDS 45.0%
! ACTCCATCTGAAATTCCAGC+CGG - chr7.4:80237787-80237806 None:intergenic 45.0%
! ATACCGGCTGGAATTTCAGA+TGG + chr7.4:80237781-80237800 MS.gene022125:CDS 45.0%
! ATCTGAAATTCCAGCCGGTA+TGG - chr7.4:80237782-80237801 None:intergenic 45.0%
! TAGTGGACAAAGCAAGAGCT+TGG + chr7.4:80238091-80238110 MS.gene022125:CDS 45.0%
! TTTATACTTCTGGAGACGGC+TGG - chr7.4:80237506-80237525 None:intergenic 45.0%
AAAGCAAGAGCTTGGGCTGT+TGG + chr7.4:80238099-80238118 MS.gene022125:CDS 50.0%
AAAGCTGACATGGGAGAAGG+CGG + chr7.4:80237480-80237499 MS.gene022125:CDS 50.0%
AATTTCCTCTTGTCAGCCCG+AGG - chr7.4:80238156-80238175 None:intergenic 50.0%
TCGTTAAAGACCATACCGGC+TGG + chr7.4:80237769-80237788 MS.gene022125:CDS 50.0%
TGCAACTGCAGGAACTGGTA+AGG + chr7.4:80237831-80237850 MS.gene022125:CDS 50.0%
! GGAGTACTCGATGTAGTACC+TGG + chr7.4:80237802-80237821 MS.gene022125:CDS 50.0%
! TTTGGTGCAACTGCAGGAAC+TGG + chr7.4:80237826-80237845 MS.gene022125:CDS 50.0%
CCTGCAGTTGCACCAAATCC+AGG - chr7.4:80237823-80237842 None:intergenic 55.0%
!! CCTGGATTTGGTGCAACTGC+AGG + chr7.4:80237820-80237839 MS.gene022125:CDS 55.0%
AAGGTCCTCGGGCTGACAAG+AGG + chr7.4:80238148-80238167 MS.gene022125:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 80237211 80238473 80237211 ID=MS.gene022125
chr7.4 mRNA 80237211 80238473 80237211 ID=MS.gene022125.t1;Parent=MS.gene022125
chr7.4 exon 80237211 80238473 80237211 ID=MS.gene022125.t1.exon1;Parent=MS.gene022125.t1
chr7.4 CDS 80237211 80238473 80237211 ID=cds.MS.gene022125.t1;Parent=MS.gene022125.t1
Gene Sequence

>MS.gene022125

ATGAGTTTCTACAAGAATGAAGTTGGTGGAGAAGATAGACTACAGCTGGATGAAATTATTGATGAATGTAAAACCTACTTTGCTGGAAAAGATACAACATCTAACTTGTTAACTTGGGCACTTCTTCTATTAGCAAAATATCAAGAATGGCAAAGTAAGACACGTGAAGAAATTGATGGGAGTACTAGAGAAGAAATTGAAAAAAGGGTTAATCAATTAGGCATTGACAAGGCTGTACCGAAAAAGATTCTTGAACTGATGAATGTTGAAAAGCTGACATGGGAGAAGGCGGCCAGCCGTCTCCAGAAGTATAAACTCTATCTAAAAAAAGTTAATTGTGTGGAAAACAAACAAGATCATATGGAAGCAACCTTAACTAGTAGTTCAGATGCATCCTATTTAAGAAGTGGAGTTGGAGGACATTTGCATACATTGAATCGCTATACTTTACTTGAAAACAGAAGGTGCAATGGTATTTGGTCAAGCACAAAGAAACTGCCTGGAACAAACACTTATCTTCCAAGAGAATCTATTAACCATGTTGATATTAAGGAGAGTTCGTTAAAGACCATACCGGCTGGAATTTCAGATGGAGTACTCGATGTAGTACCTGGATTTGGTGCAACTGCAGGAACTGGTAAGGTTAGCTTTACTGGTTCAACAAGACGTGAAATAATGGTATCTGCAGCTAGAAGTAATTTGAAACCAGCTTCACTAGAATTAGGAGGAAAATCACCCCTCTTAATTTTTTATAATGCTGATGTTGATAAAGCTGTTAAACTTGCTCTCATTAGCATCGTATTTAACAAGGGAGAAATTTGTGTTGCGAGTTCTCGTGTGTTTGTTCAAGAAGGAATCTATGATGAATTTGAGAAGATGTTAGTGGACAAAGCAAGAGCTTGGGCTGTTGGTGATCCTTTTGATCCTAAAGTTCAACAAGGTCCTCGGGCTGACAAGAGGAAATTTGAAAAAATTCTTTCATATATTGAGCATGGACAGAAAGATGGAGTAACTCTTTCGACAGGTAGTAAAAAAATTGGAGACAAGAGTTACTACAATGAGCCTACAATTTTCTCCAATGTTAAGGAGGCCATGCATACAACACAAGATGAGATATTTGGCCCTGTCATGGCACTCATGAAGTTCAAGACTATTGGAAAAGCCATCAGAAGTGCCAACAATAAAAGATATGGCTTAGGAGCAGTAATTGTGACAAAGAATTTGGATATAGCAAGCATCGTATCAAGGTCCATTCGAACATGA
Protein sequence

>MS.gene022125.t1

MSFYKNEVGGEDRLQLDEIIDECKTYFAGKDTTSNLLTWALLLLAKYQEWQSKTREEIDGSTREEIEKRVNQLGIDKAVPKKILELMNVEKLTWEKAASRLQKYKLYLKKVNCVENKQDHMEATLTSSSDASYLRSGVGGHLHTLNRYTLLENRRCNGIWSSTKKLPGTNTYLPRESINHVDIKESSLKTIPAGISDGVLDVVPGFGATAGTGKVSFTGSTRREIMVSAARSNLKPASLELGGKSPLLIFYNADVDKAVKLALISIVFNKGEICVASSRVFVQEGIYDEFEKMLVDKARAWAVGDPFDPKVQQGPRADKRKFEKILSYIEHGQKDGVTLSTGSKKIGDKSYYNEPTIFSNVKEAMHTTQDEIFGPVMALMKFKTIGKAIRSANNKRYGLGAVIVTKNLDIASIVSRSIRT