AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022240.t1 MTR_6g048540 65.812 117 40 0 457 573 106 222 8.45e-47 165
MS.gene022240.t1 MTR_6g048540 59.055 127 49 2 14 137 5 131 2.91e-42 153
MS.gene022240.t1 MTR_0734s0010 46.667 195 95 5 449 641 1 188 4.29e-45 159
MS.gene022240.t1 MTR_0853s0010 52.083 144 68 1 28 171 16 158 2.81e-43 153
MS.gene022240.t1 MTR_1g019850 55.634 142 55 1 32 173 65 198 1.55e-40 148
MS.gene022240.t1 MTR_4g035820 27.064 436 253 15 30 442 68 461 5.58e-34 138
MS.gene022240.t1 MTR_3g020080 53.271 107 29 2 274 376 3 92 2.10e-25 102
MS.gene022240.t1 MTR_0558s0020 26.255 259 165 5 34 268 33 289 6.69e-24 102
MS.gene022240.t1 MTR_6g051425 31.765 170 110 2 106 270 89 257 2.47e-23 102
MS.gene022240.t1 MTR_8g446020 29.091 165 112 1 109 268 85 249 3.35e-20 91.3
MS.gene022240.t1 MTR_1g070355 26.639 244 150 8 42 269 79 309 1.41e-18 90.1
MS.gene022240.t1 MTR_6g090505 24.901 253 159 5 109 349 60 293 9.33e-17 82.8
MS.gene022240.t1 MTR_3g450850 26.016 246 153 7 39 263 49 286 2.37e-15 78.6
MS.gene022240.t1 MTR_5g076070 26.820 261 166 9 18 263 32 282 1.97e-14 74.7
MS.gene022240.t1 MTR_8g466860 82.051 39 7 0 288 326 80 118 2.65e-14 70.5
MS.gene022240.t1 MTR_1g083640 22.363 237 158 5 34 246 48 282 2.81e-14 74.3
MS.gene022240.t1 MTR_7g446480 29.878 164 108 4 114 271 140 302 7.34e-13 70.5
MS.gene022240.t1 MTR_7g446510 28.834 163 111 2 114 271 155 317 1.87e-12 69.3
MS.gene022240.t1 MTR_3g006370 35.897 117 59 5 36 144 49 157 2.03e-12 67.8
MS.gene022240.t1 MTR_7g446520 25.391 256 154 6 29 269 74 307 2.89e-12 68.9
MS.gene022240.t1 MTR_2g024230 55.769 52 23 0 37 88 90 141 5.42e-12 64.7
MS.gene022240.t1 MTR_8g465780 26.667 150 98 3 295 441 1 141 2.13e-11 63.5
MS.gene022240.t1 MTR_1g013060 27.602 221 143 7 26 235 15 229 2.97e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022240.t1 AT4G38180 40.183 219 131 0 267 485 419 637 1.97e-53 196
MS.gene022240.t1 AT4G38180 34.866 261 147 7 27 271 59 312 2.16e-41 161
MS.gene022240.t1 AT2G27110 34.615 286 181 4 250 535 205 484 5.39e-48 179
MS.gene022240.t1 AT2G27110 48.438 64 33 0 208 271 51 114 6.02e-15 79.0
MS.gene022240.t1 AT2G27110 34.615 286 181 4 250 535 350 629 1.14e-47 180
MS.gene022240.t1 AT2G27110 28.621 290 157 12 1 271 1 259 1.03e-22 103
MS.gene022240.t1 AT2G27110 34.615 286 181 4 250 535 350 629 1.14e-47 180
MS.gene022240.t1 AT2G27110 28.621 290 157 12 1 271 1 259 1.03e-22 103
MS.gene022240.t1 AT4G38170 35.648 216 139 0 266 481 183 398 9.79e-45 168
MS.gene022240.t1 AT4G15090 30.183 328 209 5 273 597 401 711 3.68e-40 157
MS.gene022240.t1 AT4G15090 29.455 275 171 6 4 265 18 282 1.37e-25 112
MS.gene022240.t1 AT4G15090 30.183 328 209 5 273 597 401 711 3.68e-40 157
MS.gene022240.t1 AT4G15090 29.455 275 171 6 4 265 18 282 1.37e-25 112
MS.gene022240.t1 AT4G15090 30.183 328 209 5 273 597 401 711 3.68e-40 157
MS.gene022240.t1 AT4G15090 29.455 275 171 6 4 265 18 282 1.37e-25 112
MS.gene022240.t1 AT4G15090 30.183 328 209 5 273 597 401 711 3.68e-40 157
MS.gene022240.t1 AT4G15090 29.455 275 171 6 4 265 18 282 1.37e-25 112
MS.gene022240.t1 AT4G15090 30.183 328 209 5 273 597 401 711 3.68e-40 157
MS.gene022240.t1 AT4G15090 29.455 275 171 6 4 265 18 282 1.37e-25 112
MS.gene022240.t1 AT3G22170 34.562 217 141 1 265 481 402 617 7.21e-39 154
MS.gene022240.t1 AT3G22170 29.286 280 159 8 11 271 38 297 3.56e-27 117
MS.gene022240.t1 AT3G22170 34.562 217 141 1 265 481 402 617 7.21e-39 154
MS.gene022240.t1 AT3G22170 29.286 280 159 8 11 271 38 297 3.56e-27 117
MS.gene022240.t1 AT4G19990 34.783 230 148 2 262 490 338 566 1.29e-36 147
MS.gene022240.t1 AT4G19990 30.556 252 127 8 34 271 18 235 1.89e-21 99.8
MS.gene022240.t1 AT4G19990 34.783 230 148 2 262 490 338 566 1.29e-36 147
MS.gene022240.t1 AT4G19990 30.556 252 127 8 34 271 18 235 1.89e-21 99.8
MS.gene022240.t1 AT4G19990 34.783 230 148 2 262 490 281 509 1.43e-36 146
MS.gene022240.t1 AT4G19990 30.994 171 76 3 106 271 45 178 5.56e-16 82.4
MS.gene022240.t1 AT4G19990 34.783 230 148 2 262 490 338 566 1.60e-36 146
MS.gene022240.t1 AT4G19990 29.572 257 123 7 34 271 18 235 1.51e-21 100
MS.gene022240.t1 AT4G19990 34.783 230 148 2 262 490 338 566 1.76e-36 146
MS.gene022240.t1 AT4G19990 29.572 257 123 7 34 271 18 235 1.34e-21 100
MS.gene022240.t1 AT4G19990 34.783 230 148 2 262 490 338 566 1.76e-36 146
MS.gene022240.t1 AT4G19990 29.572 257 123 7 34 271 18 235 1.34e-21 100
MS.gene022240.t1 AT4G19990 34.783 230 148 2 262 490 352 580 2.02e-36 146
MS.gene022240.t1 AT4G19990 29.572 257 123 7 34 271 32 249 1.58e-21 100
MS.gene022240.t1 AT3G06250 31.365 271 180 3 265 535 490 754 2.17e-35 143
MS.gene022240.t1 AT3G06250 29.388 245 119 8 35 270 188 387 3.40e-21 99.0
MS.gene022240.t1 AT3G06250 31.365 271 180 3 265 535 490 754 2.17e-35 143
MS.gene022240.t1 AT3G06250 29.388 245 119 8 35 270 188 387 3.40e-21 99.0
MS.gene022240.t1 AT3G06250 31.365 271 180 3 265 535 490 754 2.17e-35 143
MS.gene022240.t1 AT3G06250 29.388 245 119 8 35 270 188 387 3.40e-21 99.0
MS.gene022240.t1 AT5G18960 32.103 271 178 3 265 535 514 778 4.92e-35 142
MS.gene022240.t1 AT5G18960 27.935 247 125 9 34 270 208 411 1.16e-17 87.8
MS.gene022240.t1 AT5G18960 32.103 271 178 3 265 535 514 778 4.92e-35 142
MS.gene022240.t1 AT5G18960 27.935 247 125 9 34 270 208 411 1.16e-17 87.8
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 345 614 7.42e-35 141
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 345 614 7.42e-35 141
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 345 614 7.42e-35 141
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 345 614 7.59e-35 141
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 345 614 7.59e-35 141
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 345 614 7.62e-35 141
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 304 573 9.45e-35 141
MS.gene022240.t1 AT2G32250 28.261 276 192 2 273 548 304 573 9.77e-35 141
MS.gene022240.t1 AT1G76320 26.801 347 237 6 261 599 326 663 4.41e-34 138
MS.gene022240.t1 AT1G76320 30.672 238 145 5 41 271 1 225 2.01e-25 112
MS.gene022240.t1 AT1G76320 26.513 347 238 6 261 599 326 663 5.68e-34 138
MS.gene022240.t1 AT1G76320 30.672 238 145 5 41 271 1 225 2.01e-25 112
MS.gene022240.t1 AT1G76320 26.513 347 238 6 261 599 326 663 5.68e-34 138
MS.gene022240.t1 AT1G76320 30.672 238 145 5 41 271 1 225 2.01e-25 112
MS.gene022240.t1 AT1G76320 26.513 347 238 6 261 599 326 663 6.34e-34 138
MS.gene022240.t1 AT1G76320 30.672 238 145 5 41 271 1 225 2.09e-25 112
MS.gene022240.t1 AT1G52520 33.478 230 143 4 263 483 411 639 1.04e-33 137
MS.gene022240.t1 AT1G52520 26.897 290 176 9 37 309 85 355 1.70e-20 96.7
MS.gene022240.t1 AT1G10240 26.667 300 185 5 261 545 391 670 2.55e-26 114
MS.gene022240.t1 AT1G10240 30.000 250 149 7 34 266 45 285 2.39e-24 108
MS.gene022240.t1 AT1G10240 26.667 300 185 5 261 545 391 670 2.55e-26 114
MS.gene022240.t1 AT1G10240 30.000 250 149 7 34 266 45 285 2.39e-24 108
MS.gene022240.t1 AT1G80010 29.565 230 150 5 263 483 436 662 1.32e-25 112
MS.gene022240.t1 AT1G80010 31.010 287 150 10 7 271 74 334 1.09e-23 106
MS.gene022240.t1 AT5G28530 28.111 217 146 3 261 477 343 549 8.10e-20 94.4
MS.gene022240.t1 AT5G28530 27.007 274 172 6 261 518 406 667 2.02e-19 93.2
MS.gene022240.t1 AT5G28530 24.275 276 173 7 37 289 55 317 3.04e-19 92.4
MS.gene022240.t1 AT5G28530 27.007 274 172 6 261 518 406 667 2.02e-19 93.2
MS.gene022240.t1 AT5G28530 24.275 276 173 7 37 289 55 317 3.04e-19 92.4
MS.gene022240.t1 AT5G28530 28.507 221 144 4 261 477 343 553 2.89e-19 92.4
MS.gene022240.t1 AT5G28530 24.275 276 173 7 37 289 55 317 3.06e-19 92.4
MS.gene022240.t1 AT5G28530 27.338 278 170 7 261 518 406 671 7.14e-19 91.3
MS.gene022240.t1 AT2G43280 31.159 138 79 5 19 148 4 133 1.30e-11 64.7
MS.gene022240.t1 AT3G07500 31.544 149 80 7 1 141 1 135 3.16e-11 63.9

Find 82 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTCGTTGATGTAAATT+TGG 0.172325 3.3:+66745083 MS.gene022240:CDS
CTTCCTCCGACAAGCAATTT+TGG 0.188112 3.3:-66745497 None:intergenic
TTTACAATTCATTTGCAAAA+AGG 0.228560 3.3:+66743625 MS.gene022240:CDS
TGCATCCTTCTTAATATCTT+TGG 0.239826 3.3:-66745303 None:intergenic
GGGACTTGGCTTTCTCTAAT+AGG 0.243998 3.3:+66744018 MS.gene022240:CDS
AGATTAGCTTACCAACAATT+TGG 0.249704 3.3:+66744215 MS.gene022240:CDS
AGCAAAGGTATTAGTTGATT+TGG 0.254141 3.3:+66745338 MS.gene022240:CDS
GAGGTACATTAGAAGGAAAT+TGG 0.278387 3.3:+66743829 MS.gene022240:CDS
GGAGTTTCTCCTTTGGAATC+TGG 0.287253 3.3:+66745462 MS.gene022240:CDS
GGATTTCTAATAGTCAATTC+TGG 0.312090 3.3:-66745528 None:intergenic
ATCTTGAGCATTGTAGTTGC+TGG 0.330448 3.3:-66745058 None:intergenic
GGTAAATTTCAAGATGAGAT+TGG 0.337382 3.3:+66744970 MS.gene022240:CDS
CATACAAAGACAGCTCTCTT+TGG 0.340587 3.3:-66743681 None:intergenic
GGACTTGGCTTTCTCTAATA+GGG 0.341505 3.3:+66744019 MS.gene022240:CDS
TATATAAAATTAGAGAATCT+TGG 0.344745 3.3:+66744686 MS.gene022240:CDS
TTTAATTCCTCACCAACATT+AGG 0.347135 3.3:-66743549 None:intergenic
ATTAAATAGTGAACCTTGTA+AGG 0.348615 3.3:+66743566 MS.gene022240:CDS
CAGTGATGGAGTTTCTCCTT+TGG 0.356600 3.3:+66745455 MS.gene022240:CDS
TCCAACGCTGCAAAATAAAA+TGG 0.360936 3.3:-66745212 None:intergenic
GTTAAAAGAGGTACATTAGA+AGG 0.366674 3.3:+66743822 MS.gene022240:CDS
TAGTTTGATTTGTGTCATCT+TGG 0.378031 3.3:-66743752 None:intergenic
CTTTCTCTAATAGGGATTGT+TGG 0.385066 3.3:+66744027 MS.gene022240:CDS
AAACAGCTTCTATTATAAAC+TGG 0.392497 3.3:-66744712 None:intergenic
CAATTATTGCAAACAGAAAC+AGG 0.397066 3.3:+66744109 MS.gene022240:CDS
GAAACTTGATAGACATAGTT+AGG 0.397106 3.3:-66745036 None:intergenic
ATATTACATCTCCAAATTGT+TGG 0.402900 3.3:-66744226 None:intergenic
AATGTTCAGTGAAGGGGACT+TGG 0.418030 3.3:+66744004 MS.gene022240:CDS
GTAAATTTCAAGATGAGATT+GGG 0.418089 3.3:+66744971 MS.gene022240:CDS
GTCCGTACTCAATCATTAAT+CGG 0.426232 3.3:-66744636 None:intergenic
AAGTGTTCTACTTCTCGAAC+TGG 0.426697 3.3:+66743780 MS.gene022240:CDS
TCCAATGTGATGAAGATTCT+CGG 0.434696 3.3:+66744159 MS.gene022240:CDS
CCACGCAATGCACAAACAAA+AGG 0.435072 3.3:+66745549 MS.gene022240:CDS
GCCACACTTGTTGTTAAAAG+AGG 0.437725 3.3:+66743810 MS.gene022240:CDS
GACAACAATGAATGGCTTCA+AGG 0.439948 3.3:+66744661 MS.gene022240:CDS
AATTCATTATATCTGATTTG+AGG 0.445916 3.3:+66745385 MS.gene022240:CDS
CGCTGCAAAATAAAATGGTC+AGG 0.446582 3.3:-66745207 None:intergenic
GGAATTCAAATCTGTGGAAA+AGG 0.451124 3.3:+66743593 MS.gene022240:CDS
TGTCATGAGTGTGGTGAATC+TGG 0.451337 3.3:+66745669 MS.gene022240:CDS
ATTTGTGTTGAAATTTGAAA+AGG 0.454017 3.3:+66744822 MS.gene022240:CDS
TTGAATTCCATCCCCTTACA+AGG 0.484462 3.3:-66743579 None:intergenic
TTAGCTGCTGCTCCTAATGT+TGG 0.490364 3.3:+66743537 MS.gene022240:CDS
AAAGAGAGGATTATGAAAGT+AGG 0.500092 3.3:+66744872 MS.gene022240:CDS
ATTTGATTTCTTCATTGCAA+TGG 0.501662 3.3:-66745424 None:intergenic
CAAAATTGTAGATTCAAAAG+TGG 0.511466 3.3:+66745600 MS.gene022240:CDS
GTCTTTGTATGTTGTAATGA+AGG 0.517879 3.3:+66743693 MS.gene022240:CDS
TGATTTGAGGCGCACTCGTA+TGG 0.520232 3.3:+66745398 MS.gene022240:CDS
ATACTTGTTAGTCTTATATG+TGG 0.524306 3.3:-66744258 None:intergenic
TCCGAGAATCTTCATCACAT+TGG 0.530876 3.3:-66744160 None:intergenic
TAGTGAACCTTGTAAGGGGA+TGG 0.531310 3.3:+66743572 MS.gene022240:CDS
CACGCAATGCACAAACAAAA+GGG 0.538817 3.3:+66745550 MS.gene022240:CDS
AAGGAAGAAGGTATACCGAC+AGG 0.538923 3.3:+66743969 MS.gene022240:CDS
TGTCAACCAAAATTGCTTGT+CGG 0.544243 3.3:+66745491 MS.gene022240:CDS
CGGTGTCATAAAAGCATGAA+TGG 0.550442 3.3:+66743918 MS.gene022240:CDS
TCAAATTTAGCTATCAGTGA+TGG 0.553213 3.3:+66745441 MS.gene022240:CDS
CGAAGAAAGAATTTAGACGT+TGG 0.559738 3.3:+66744068 MS.gene022240:CDS
TTAAATAGTGAACCTTGTAA+GGG 0.571595 3.3:+66743567 MS.gene022240:CDS
GCACGCCAAAGATATTAAGA+AGG 0.572798 3.3:+66745298 MS.gene022240:CDS
ACAACAATGAATGGCTTCAA+GGG 0.573168 3.3:+66744662 MS.gene022240:CDS
ACGGACTGGACAACAATGAA+TGG 0.575271 3.3:+66744653 MS.gene022240:CDS
TGCTACAATGTTCAGTGAAG+GGG 0.575975 3.3:+66743998 MS.gene022240:CDS
TTGCTACAATGTTCAGTGAA+GGG 0.579493 3.3:+66743997 MS.gene022240:CDS
GTCTACAGGGTCAAAACTTG+AGG 0.581238 3.3:+66744912 MS.gene022240:CDS
GGGGATGGAATTCAAATCTG+TGG 0.582477 3.3:+66743587 MS.gene022240:CDS
TGTGTTGAAATTTGAAAAGG+CGG 0.586171 3.3:+66744825 MS.gene022240:CDS
AGCCGATTAATGATTGAGTA+CGG 0.595364 3.3:+66744634 MS.gene022240:CDS
GAAACACAGATTGCAAAATG+TGG 0.599828 3.3:+66745105 MS.gene022240:CDS
ATTGTAGCAACCTTTCCTGT+CGG 0.600908 3.3:-66743984 None:intergenic
ACAAACAAAAGGGAGAAAGA+AGG 0.601052 3.3:+66745560 MS.gene022240:CDS
TGCTGCTCCTAATGTTGGTG+AGG 0.601546 3.3:+66743542 MS.gene022240:CDS
GTTGCTACAATGTTCAGTGA+AGG 0.603702 3.3:+66743996 MS.gene022240:CDS
GCATGCTCAACAAGAAGCAA+AGG 0.605686 3.3:+66745323 MS.gene022240:CDS
TTCCACATTTGTTGACTGTG+TGG 0.606600 3.3:-66743496 None:intergenic
ATTAATGATTGAGTACGGAC+TGG 0.607432 3.3:+66744639 MS.gene022240:CDS
GTGCATTGGTCAATTATGGA+TGG 0.620199 3.3:+66743456 None:intergenic
AGAAGGTATACCGACAGGAA+AGG 0.621900 3.3:+66743974 MS.gene022240:CDS
AGCCACACAGTCAACAAATG+TGG 0.622019 3.3:+66743494 MS.gene022240:CDS
ACAAAAGGGAGAAAGAAGGA+TGG 0.625355 3.3:+66745564 MS.gene022240:CDS
TAAATAGTGAACCTTGTAAG+GGG 0.625905 3.3:+66743568 MS.gene022240:CDS
CAACCAAAATTGCTTGTCGG+AGG 0.633822 3.3:+66745494 MS.gene022240:CDS
GAATGATCATAACCATCCCA+TGG 0.640152 3.3:+66743869 MS.gene022240:CDS
CTAAAAGTGTGTCTTATATG+CGG 0.670716 3.3:+66743898 MS.gene022240:CDS
AGAAAAGCATGTCATGAGTG+TGG 0.709074 3.3:+66745660 MS.gene022240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAAAATTAGAGAATCT+TGG + chr3.3:66744686-66744705 MS.gene022240:CDS 15.0%
!!! CATTATCAATCAATTTTATT+TGG + chr3.3:66744317-66744336 MS.gene022240:intron 15.0%
!! ATTTGTGTTGAAATTTGAAA+AGG + chr3.3:66744822-66744841 MS.gene022240:CDS 20.0%
!! TTTACAATTCATTTGCAAAA+AGG + chr3.3:66743625-66743644 MS.gene022240:CDS 20.0%
!!! AATTCATTATATCTGATTTG+AGG + chr3.3:66745385-66745404 MS.gene022240:CDS 20.0%
!!! ATGTCAATTATATGAGTTTT+TGG + chr3.3:66745128-66745147 MS.gene022240:CDS 20.0%
!!! ATTTATTCGAGAAACATTTT+TGG + chr3.3:66744949-66744968 MS.gene022240:CDS 20.0%
!!! TGTCAATTATATGAGTTTTT+GGG + chr3.3:66745129-66745148 MS.gene022240:CDS 20.0%
! AAAAAGAAGATTAGCAAAGA+TGG + chr3.3:66745012-66745031 MS.gene022240:CDS 25.0%
! AAACAGCTTCTATTATAAAC+TGG - chr3.3:66744715-66744734 None:intergenic 25.0%
! AATTCATTTGCAAAAAGGAT+GGG + chr3.3:66743630-66743649 MS.gene022240:CDS 25.0%
! ATATTACATCTCCAAATTGT+TGG - chr3.3:66744229-66744248 None:intergenic 25.0%
! ATTAAATAGTGAACCTTGTA+AGG + chr3.3:66743566-66743585 MS.gene022240:CDS 25.0%
! CAAAATTGTAGATTCAAAAG+TGG + chr3.3:66745600-66745619 MS.gene022240:CDS 25.0%
! GTAAATTTCAAGATGAGATT+GGG + chr3.3:66744971-66744990 MS.gene022240:CDS 25.0%
! TCTTCAAAACTTTCTATACT+TGG - chr3.3:66744607-66744626 None:intergenic 25.0%
! TGATTATTCAATCCAACAAA+CGG - chr3.3:66744299-66744318 None:intergenic 25.0%
! TTAAATAGTGAACCTTGTAA+GGG + chr3.3:66743567-66743586 MS.gene022240:CDS 25.0%
!! ATTTGATTTCTTCATTGCAA+TGG - chr3.3:66745427-66745446 None:intergenic 25.0%
!! GTCAATTATATGAGTTTTTG+GGG + chr3.3:66745130-66745149 MS.gene022240:CDS 25.0%
!!! AAGTCTTGTTGAACATTTTA+AGG + chr3.3:66743950-66743969 MS.gene022240:CDS 25.0%
!!! ATACTTGTTAGTCTTATATG+TGG - chr3.3:66744261-66744280 None:intergenic 25.0%
!!! GTTGTTTGAAACTTTTCTTA+AGG + chr3.3:66744382-66744401 MS.gene022240:intron 25.0%
!!! TCGGATGATAAACTTTTTTT+GGG + chr3.3:66744178-66744197 MS.gene022240:CDS 25.0%
AAAAAACGCATATTGTCTAC+AGG + chr3.3:66744898-66744917 MS.gene022240:CDS 30.0%
AAAAACGCATATTGTCTACA+GGG + chr3.3:66744899-66744918 MS.gene022240:CDS 30.0%
AAAGAGAGGATTATGAAAGT+AGG + chr3.3:66744872-66744891 MS.gene022240:CDS 30.0%
ATTATTCAATCCAACAAACG+GGG - chr3.3:66744297-66744316 None:intergenic 30.0%
CAATTATTGCAAACAGAAAC+AGG + chr3.3:66744109-66744128 MS.gene022240:CDS 30.0%
CAATTCATTTGCAAAAAGGA+TGG + chr3.3:66743629-66743648 MS.gene022240:CDS 30.0%
CTTAGAAGTAGAAAAAAGAG+AGG + chr3.3:66744858-66744877 MS.gene022240:CDS 30.0%
GAAACTTGATAGACATAGTT+AGG - chr3.3:66745039-66745058 None:intergenic 30.0%
GATTATTCAATCCAACAAAC+GGG - chr3.3:66744298-66744317 None:intergenic 30.0%
GGATTTCTAATAGTCAATTC+TGG - chr3.3:66745531-66745550 None:intergenic 30.0%
GGTAAATTTCAAGATGAGAT+TGG + chr3.3:66744970-66744989 MS.gene022240:CDS 30.0%
GTCTTTGTATGTTGTAATGA+AGG + chr3.3:66743693-66743712 MS.gene022240:CDS 30.0%
GTTTCTCGTTGATGTAAATT+TGG + chr3.3:66745083-66745102 MS.gene022240:CDS 30.0%
TAAATAGTGAACCTTGTAAG+GGG + chr3.3:66743568-66743587 MS.gene022240:CDS 30.0%
TCAAATTTAGCTATCAGTGA+TGG + chr3.3:66745441-66745460 MS.gene022240:CDS 30.0%
TGCATCCTTCTTAATATCTT+TGG - chr3.3:66745306-66745325 None:intergenic 30.0%
TGTGTTGAAATTTGAAAAGG+CGG + chr3.3:66744825-66744844 MS.gene022240:CDS 30.0%
TTTAATTCCTCACCAACATT+AGG - chr3.3:66743552-66743571 None:intergenic 30.0%
! CTAAAAGTGTGTCTTATATG+CGG + chr3.3:66743898-66743917 MS.gene022240:CDS 30.0%
! GCATATAAGACACACTTTTA+GGG - chr3.3:66743899-66743918 None:intergenic 30.0%
! GTTAAAAGAGGTACATTAGA+AGG + chr3.3:66743822-66743841 MS.gene022240:CDS 30.0%
! TGAAACTTTTCTTAAGGCAA+TGG + chr3.3:66744388-66744407 MS.gene022240:intron 30.0%
!! AGATTAGCTTACCAACAATT+TGG + chr3.3:66744215-66744234 MS.gene022240:CDS 30.0%
!! AGCAAAGGTATTAGTTGATT+TGG + chr3.3:66745338-66745357 MS.gene022240:CDS 30.0%
!! GTTGAACATTTTAAGGAAGA+AGG + chr3.3:66743957-66743976 MS.gene022240:CDS 30.0%
!! TAGTTTGATTTGTGTCATCT+TGG - chr3.3:66743755-66743774 None:intergenic 30.0%
!!! AATAGAAGCTGTTTTTTTGC+TGG + chr3.3:66744721-66744740 MS.gene022240:CDS 30.0%
!!! AGCAATTTTGGTTGACATTT+TGG - chr3.3:66745488-66745507 None:intergenic 30.0%
!!! ATAGAAGCTGTTTTTTTGCT+GGG + chr3.3:66744722-66744741 MS.gene022240:CDS 30.0%
!!! CTCGGATGATAAACTTTTTT+TGG + chr3.3:66744177-66744196 MS.gene022240:CDS 30.0%
!!! CTCGTAATTTTTCAAGCAAA+AGG + chr3.3:66745171-66745190 MS.gene022240:CDS 30.0%
!!! CTCTTTTTTCTACTTCTAAG+CGG - chr3.3:66744858-66744877 None:intergenic 30.0%
!!! TAGAAAGTTTTGAAGAAGAC+TGG + chr3.3:66744611-66744630 MS.gene022240:intron 30.0%
ACAAACAAAAGGGAGAAAGA+AGG + chr3.3:66745560-66745579 MS.gene022240:CDS 35.0%
ACAACAATGAATGGCTTCAA+GGG + chr3.3:66744662-66744681 MS.gene022240:CDS 35.0%
AGCCGATTAATGATTGAGTA+CGG + chr3.3:66744634-66744653 MS.gene022240:CDS 35.0%
AGTCAGAACGCTCATTTATA+TGG + chr3.3:66744360-66744379 MS.gene022240:intron 35.0%
ATTAATGATTGAGTACGGAC+TGG + chr3.3:66744639-66744658 MS.gene022240:CDS 35.0%
CGAAGAAAGAATTTAGACGT+TGG + chr3.3:66744068-66744087 MS.gene022240:CDS 35.0%
CTTTCTCTAATAGGGATTGT+TGG + chr3.3:66744027-66744046 MS.gene022240:CDS 35.0%
GAAACACAGATTGCAAAATG+TGG + chr3.3:66745105-66745124 MS.gene022240:CDS 35.0%
GAGGTACATTAGAAGGAAAT+TGG + chr3.3:66743829-66743848 MS.gene022240:CDS 35.0%
GGAATTCAAATCTGTGGAAA+AGG + chr3.3:66743593-66743612 MS.gene022240:CDS 35.0%
GTCCGTACTCAATCATTAAT+CGG - chr3.3:66744639-66744658 None:intergenic 35.0%
TCCAACGCTGCAAAATAAAA+TGG - chr3.3:66745215-66745234 None:intergenic 35.0%
TCCAATGTGATGAAGATTCT+CGG + chr3.3:66744159-66744178 MS.gene022240:CDS 35.0%
TGGTCAGTTATGACAGATTT+GGG - chr3.3:66744425-66744444 None:intergenic 35.0%
TGTCAACCAAAATTGCTTGT+CGG + chr3.3:66745491-66745510 MS.gene022240:CDS 35.0%
TTGCTACAATGTTCAGTGAA+GGG + chr3.3:66743997-66744016 MS.gene022240:CDS 35.0%
TTTCAATTCTCGCTTGAAAG+TGG - chr3.3:66744567-66744586 None:intergenic 35.0%
! ACCTCTTTTAACAACAAGTG+TGG - chr3.3:66743814-66743833 None:intergenic 35.0%
! ACTTTTCTTAAGGCAATGGA+TGG + chr3.3:66744392-66744411 MS.gene022240:intron 35.0%
! CGCATATAAGACACACTTTT+AGG - chr3.3:66743900-66743919 None:intergenic 35.0%
! GATACGACGAGTAATTTTGA+AGG + chr3.3:66745267-66745286 MS.gene022240:CDS 35.0%
!! TTTGCAAAAAGGATGGGTTT+TGG + chr3.3:66743636-66743655 MS.gene022240:CDS 35.0%
!!! ACCATTTTATTTTGCAGCGT+TGG + chr3.3:66745211-66745230 MS.gene022240:CDS 35.0%
AAGTGTTCTACTTCTCGAAC+TGG + chr3.3:66743780-66743799 MS.gene022240:CDS 40.0%
ACAAAAGGGAGAAAGAAGGA+TGG + chr3.3:66745564-66745583 MS.gene022240:CDS 40.0%
ATTGTAGCAACCTTTCCTGT+CGG - chr3.3:66743987-66744006 None:intergenic 40.0%
CAAATCTGTCATAACTGACC+AGG + chr3.3:66744424-66744443 MS.gene022240:intron 40.0%
CACGCAATGCACAAACAAAA+GGG + chr3.3:66745550-66745569 MS.gene022240:CDS 40.0%
CATACAAAGACAGCTCTCTT+TGG - chr3.3:66743684-66743703 None:intergenic 40.0%
CGCTGCAAAATAAAATGGTC+AGG - chr3.3:66745210-66745229 None:intergenic 40.0%
CTGGTCAGTTATGACAGATT+TGG - chr3.3:66744426-66744445 None:intergenic 40.0%
GAATGATCATAACCATCCCA+TGG + chr3.3:66743869-66743888 MS.gene022240:CDS 40.0%
GACAACAATGAATGGCTTCA+AGG + chr3.3:66744661-66744680 MS.gene022240:CDS 40.0%
GCACGCCAAAGATATTAAGA+AGG + chr3.3:66745298-66745317 MS.gene022240:CDS 40.0%
GTTGCTACAATGTTCAGTGA+AGG + chr3.3:66743996-66744015 MS.gene022240:CDS 40.0%
TCCGAGAATCTTCATCACAT+TGG - chr3.3:66744163-66744182 None:intergenic 40.0%
TCCTAATATGCCATAAGCAG+AGG - chr3.3:66744502-66744521 None:intergenic 40.0%
TGCTACAATGTTCAGTGAAG+GGG + chr3.3:66743998-66744017 MS.gene022240:CDS 40.0%
TGTCATAACTGACCAGGATT+TGG + chr3.3:66744430-66744449 MS.gene022240:intron 40.0%
TTGAATTCCATCCCCTTACA+AGG - chr3.3:66743582-66743601 None:intergenic 40.0%
! AGAAAAGCATGTCATGAGTG+TGG + chr3.3:66745660-66745679 MS.gene022240:CDS 40.0%
! ATCTTGAGCATTGTAGTTGC+TGG - chr3.3:66745061-66745080 None:intergenic 40.0%
! GGACTTGGCTTTCTCTAATA+GGG + chr3.3:66744019-66744038 MS.gene022240:CDS 40.0%
! TTCCACATTTGTTGACTGTG+TGG - chr3.3:66743499-66743518 None:intergenic 40.0%
! TTGGCTATGAAAGCTGCTTT+AGG + chr3.3:66744449-66744468 MS.gene022240:intron 40.0%
!! ACATTTTGGCCAGATTCCAA+AGG - chr3.3:66745474-66745493 None:intergenic 40.0%
!! AGTTTTTGGGGATATTGTGC+AGG + chr3.3:66745142-66745161 MS.gene022240:CDS 40.0%
!! CGGTGTCATAAAAGCATGAA+TGG + chr3.3:66743918-66743937 MS.gene022240:CDS 40.0%
!! GCCACACTTGTTGTTAAAAG+AGG + chr3.3:66743810-66743829 MS.gene022240:CDS 40.0%
!!! ACACTTTTAGGGCTAACCAT+GGG - chr3.3:66743888-66743907 None:intergenic 40.0%
AATCCAACAAACGGGGCAAA+TGG - chr3.3:66744290-66744309 None:intergenic 45.0%
AATGTTCAGTGAAGGGGACT+TGG + chr3.3:66744004-66744023 MS.gene022240:CDS 45.0%
AGCCACACAGTCAACAAATG+TGG + chr3.3:66743494-66743513 MS.gene022240:CDS 45.0%
CAACCAAAATTGCTTGTCGG+AGG + chr3.3:66745494-66745513 MS.gene022240:CDS 45.0%
CAGCTTTCATAGCCAAATCC+TGG - chr3.3:66744445-66744464 None:intergenic 45.0%
CCACGCAATGCACAAACAAA+AGG + chr3.3:66745549-66745568 MS.gene022240:CDS 45.0%
GCATGCTCAACAAGAAGCAA+AGG + chr3.3:66745323-66745342 MS.gene022240:CDS 45.0%
GCCTCTGCTTATGGCATATT+AGG + chr3.3:66744498-66744517 MS.gene022240:intron 45.0%
GGGGATGGAATTCAAATCTG+TGG + chr3.3:66743587-66743606 MS.gene022240:CDS 45.0%
GTCTACAGGGTCAAAACTTG+AGG + chr3.3:66744912-66744931 MS.gene022240:CDS 45.0%
TAGTGAACCTTGTAAGGGGA+TGG + chr3.3:66743572-66743591 MS.gene022240:CDS 45.0%
TGTCATGAGTGTGGTGAATC+TGG + chr3.3:66745669-66745688 MS.gene022240:CDS 45.0%
TTAGCTGCTGCTCCTAATGT+TGG + chr3.3:66743537-66743556 MS.gene022240:CDS 45.0%
! AAGGAAGAAGGTATACCGAC+AGG + chr3.3:66743969-66743988 MS.gene022240:CDS 45.0%
! AGAAGGTATACCGACAGGAA+AGG + chr3.3:66743974-66743993 MS.gene022240:CDS 45.0%
! CAGTGATGGAGTTTCTCCTT+TGG + chr3.3:66745455-66745474 MS.gene022240:CDS 45.0%
! CTTCCTCCGACAAGCAATTT+TGG - chr3.3:66745500-66745519 None:intergenic 45.0%
! GGGACTTGGCTTTCTCTAAT+AGG + chr3.3:66744018-66744037 MS.gene022240:CDS 45.0%
!! ACGGACTGGACAACAATGAA+TGG + chr3.3:66744653-66744672 MS.gene022240:CDS 45.0%
!! CCTTTTGTTTGTGCATTGCG+TGG - chr3.3:66745552-66745571 None:intergenic 45.0%
!! GGAGTTTCTCCTTTGGAATC+TGG + chr3.3:66745462-66745481 MS.gene022240:CDS 45.0%
!!! CACACTTTTAGGGCTAACCA+TGG - chr3.3:66743889-66743908 None:intergenic 45.0%
!!! TTTTAGGGCTAACCATGGGA+TGG - chr3.3:66743884-66743903 None:intergenic 45.0%
CTAGACATCGCCTCTGCTTA+TGG + chr3.3:66744489-66744508 MS.gene022240:intron 50.0%
TGCTGCTCCTAATGTTGGTG+AGG + chr3.3:66743542-66743561 MS.gene022240:CDS 50.0%
! ATGCCATTTGCCCCGTTTGT+TGG + chr3.3:66744284-66744303 MS.gene022240:intron 50.0%
! CAGAGGCGATGTCTAGTTTC+TGG - chr3.3:66744485-66744504 None:intergenic 50.0%
! TGATTTGAGGCGCACTCGTA+TGG + chr3.3:66745398-66745417 MS.gene022240:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 66743471 66745734 66743471 ID=MS.gene022240
chr3.3 mRNA 66743471 66745734 66743471 ID=MS.gene022240.t1;Parent=MS.gene022240
chr3.3 exon 66743471 66744281 66743471 ID=MS.gene022240.t1.exon1;Parent=MS.gene022240.t1
chr3.3 CDS 66743471 66744281 66743471 ID=cds.MS.gene022240.t1;Parent=MS.gene022240.t1
chr3.3 exon 66744614 66745734 66744614 ID=MS.gene022240.t1.exon2;Parent=MS.gene022240.t1
chr3.3 CDS 66744614 66745734 66744614 ID=cds.MS.gene022240.t1;Parent=MS.gene022240.t1
Gene Sequence

>MS.gene022240

ATGGATGGTAATGTTTTTGAAGAAGCCACACAGTCAACAAATGTGGAAATATCTGAAGAAGATATTTTAGCTGCTGCTCCTAATGTTGGTGAGGAATTAAATAGTGAACCTTGTAAGGGGATGGAATTCAAATCTGTGGAAAAGGTTAAAGAATTTTACAATTCATTTGCAAAAAGGATGGGTTTTGGAGTACGTGTTCGCTCAACTAAACCAAAGAGAGCTGTCTTTGTATGTTGTAATGAAGGTCATCATATAGTGAAAAGTTCAATAAACAAAGAAATCCAAGATGACACAAATCAAACTAAAAGAAAGTGTTCTACTTCTCGAACTGGTTGTCTAGCCACACTTGTTGTTAAAAGAGGTACATTAGAAGGAAATTGGATTATATGTTCATTTAAGAATGATCATAACCATCCCATGGTTAGCCCTAAAAGTGTGTCTTATATGCGGTGTCATAAAAGCATGAATGGTGCTGCAAAAAGTCTTGTTGAACATTTTAAGGAAGAAGGTATACCGACAGGAAAGGTTGCTACAATGTTCAGTGAAGGGGACTTGGCTTTCTCTAATAGGGATTGTTGGAATCATGTCAGAAGTCTTCGAAGAAAGAATTTAGACGTTGGAGATGCTCAAGCTGTTTTCAATTATTGCAAACAGAAACAGGTAGAAAATTCAAACTTTTTCTATGAAATCCAATGTGATGAAGATTCTCGGATGATAAACTTTTTTTGGGTTGATGCTAGATCAAGATTAGCTTACCAACAATTTGGAGATGTAATATCTTTTGACACCACATATAAGACTAACAAGTATAGTATGCCATTTGCCCCGTTTGTTGGATTGAATAATCATTATCAATCAATTTTATTTGGTTGTGCTTTACTACAAGACGAGTCAGAACGCTCATTTATATGGTTGTTTGAAACTTTTCTTAAGGCAATGGATGGTAAAAAGCCCAAATCTGTCATAACTGACCAGGATTTGGCTATGAAAGCTGCTTTAGGCAAAGTATTTCCAGAAACTAGACATCGCCTCTGCTTATGGCATATTAGGAAAAAATTTGCTGAAAAGTTAGCTCATGTTTATCATAAAAATTCCACTTTCAAGCGAGAATTGAAAAAATGCATTCATTTTCACCAAGTATAGAAAGTTTTGAAGAAGACTGGAGCCGATTAATGATTGAGTACGGACTGGACAACAATGAATGGCTTCAAGGGTTATATAAAATTAGAGAATCTTGGATACCAGTTTATAATAGAAGCTGTTTTTTTGCTGGGATGAATACAAGTCAAAGAAGTGAGAGCATCAATGCGTTTTTTGATTCTTTTGTGAATGCAACTACAACATTGCAAGAATTTGTGTTGAAATTTGAAAAGGCGGTTGATAGCCGCTTAGAAGTAGAAAAAAGAGAGGATTATGAAAGTAGGCATAAAAAACGCATATTGTCTACAGGGTCAAAACTTGAGGAGCACGCTGCGTCTATTTATTCGAGAAACATTTTTGGTAAATTTCAAGATGAGATTGGGAAGATCTACAAATTTGCAAAAAAGAAGATTAGCAAAGATGGACCTAACTATGTCTATCAAGTTTCCAGCAACTACAATGCTCAAGATATGTTTCTCGTTGATGTAAATTTGGAAACACAGATTGCAAAATGTGGATGTCAATTATATGAGTTTTTGGGGATATTGTGCAGGCATATACTCGTAATTTTTCAAGCAAAAGGTATCACTCAAATTCCTGACCATTTTATTTTGCAGCGTTGGACTAAAAATGCTAACAAAAGCATTGAAGTTTGTGATACGACGAGTAATTTTGAAGGTCAAAATAGCACGCCAAAGATATTAAGAAGGATGCATGCTCAACAAGAAGCAAAGGTATTAGTTGATTTGGCTGAAGAGTCTGATGAGATATACAAATTCATTATATCTGATTTGAGGCGCACTCGTATGGCAGCCATTGCAATGAAGAAATCAAATTTAGCTATCAGTGATGGAGTTTCTCCTTTGGAATCTGGCCAAAATGTCAACCAAAATTGCTTGTCGGAGGAAGAAAGTGAACCAGAATTGACTATTAGAAATCCACGCAATGCACAAACAAAAGGGAGAAAGAAGGATGGACAGAAAAACACTCAAAATTGTAGATTCAAAAGTGGAATTGAACTGTCACATGCTCAACAAGTAGCAGTCAAAAGAAAAGCATGTCATGAGTGTGGTGAATCTGGTCACAACAGCAGAACTTGCAAGAAAAGAAATCAAAATGATTAA
Protein sequence

>MS.gene022240.t1

MDGNVFEEATQSTNVEISEEDILAAAPNVGEELNSEPCKGMEFKSVEKVKEFYNSFAKRMGFGVRVRSTKPKRAVFVCCNEGHHIVKSSINKEIQDDTNQTKRKCSTSRTGCLATLVVKRGTLEGNWIICSFKNDHNHPMVSPKSVSYMRCHKSMNGAAKSLVEHFKEEGIPTGKVATMFSEGDLAFSNRDCWNHVRSLRRKNLDVGDAQAVFNYCKQKQVENSNFFYEIQCDEDSRMINFFWVDARSRLAYQQFGDVISFDTTYKTNKYKSFEEDWSRLMIEYGLDNNEWLQGLYKIRESWIPVYNRSCFFAGMNTSQRSESINAFFDSFVNATTTLQEFVLKFEKAVDSRLEVEKREDYESRHKKRILSTGSKLEEHAASIYSRNIFGKFQDEIGKIYKFAKKKISKDGPNYVYQVSSNYNAQDMFLVDVNLETQIAKCGCQLYEFLGILCRHILVIFQAKGITQIPDHFILQRWTKNANKSIEVCDTTSNFEGQNSTPKILRRMHAQQEAKVLVDLAEESDEIYKFIISDLRRTRMAAIAMKKSNLAISDGVSPLESGQNVNQNCLSEEESEPELTIRNPRNAQTKGRKKDGQKNTQNCRFKSGIELSHAQQVAVKRKACHECGESGHNSRTCKKRNQND