AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022285

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022285.t1 MTR_3g078110 97.638 127 3 0 5 131 2 128 4.12e-86 260
MS.gene022285.t1 MTR_3g078110 99.123 114 1 0 129 242 250 363 8.35e-83 251
MS.gene022285.t1 MTR_4g106980 90.351 114 11 0 129 242 279 392 1.61e-76 236
MS.gene022285.t1 MTR_4g106980 57.500 160 42 3 1 134 1 160 3.91e-53 176
MS.gene022285.t1 MTR_6g007603 88.596 114 13 0 129 242 262 375 1.65e-74 231
MS.gene022285.t1 MTR_6g007603 55.944 143 53 2 2 134 1 143 5.09e-49 165
MS.gene022285.t1 MTR_5g006560 78.070 114 25 0 129 242 277 390 1.14e-66 211
MS.gene022285.t1 MTR_5g006560 49.612 129 54 1 6 134 37 154 5.58e-33 123
MS.gene022285.t1 MTR_5g006560 78.070 114 25 0 129 242 311 424 3.32e-66 211
MS.gene022285.t1 MTR_5g006560 49.612 129 54 1 6 134 71 188 8.69e-33 123
MS.gene022285.t1 MTR_1g103270 68.421 114 36 0 129 242 269 382 1.13e-55 182
MS.gene022285.t1 MTR_1g103270 48.872 133 56 2 2 134 29 149 6.04e-35 128
MS.gene022285.t1 MTR_4g106980 57.500 160 42 3 1 134 1 160 3.94e-54 177
MS.gene022285.t1 MTR_4g106980 95.652 46 2 0 129 174 279 324 2.71e-26 104
MS.gene022285.t1 MTR_8g100085 33.529 170 91 6 60 217 232 391 1.81e-22 95.1
MS.gene022285.t1 MTR_8g100085 36.585 82 49 2 50 131 120 198 4.59e-11 62.4
MS.gene022285.t1 MTR_3g034100 32.778 180 98 7 50 217 172 340 1.04e-21 92.4
MS.gene022285.t1 MTR_3g034100 35.714 84 51 1 50 133 69 149 4.49e-12 65.1
MS.gene022285.t1 MTR_4g088745 38.261 115 63 2 136 242 223 337 1.19e-21 92.4
MS.gene022285.t1 MTR_4g088755 38.261 115 63 2 136 242 218 332 1.41e-21 92.0
MS.gene022285.t1 MTR_4g132860 30.208 192 107 6 59 234 174 354 1.19e-20 89.7
MS.gene022285.t1 MTR_4g088750 45.238 84 46 0 136 219 379 462 6.55e-20 88.6
MS.gene022285.t1 MTR_2g013900 36.522 115 65 2 136 242 340 454 9.03e-20 87.8
MS.gene022285.t1 MTR_1g026160 38.202 89 55 0 129 217 310 398 6.96e-19 85.1
MS.gene022285.t1 MTR_4g105770 40.506 79 47 0 136 214 235 313 6.15e-18 82.0
MS.gene022285.t1 MTR_3g078000 30.233 172 98 6 60 219 156 317 7.04e-18 81.6
MS.gene022285.t1 MTR_5g023150 34.091 88 58 0 129 216 363 450 3.07e-17 80.5
MS.gene022285.t1 MTR_5g023150 34.091 88 58 0 129 216 460 547 3.52e-17 80.5
MS.gene022285.t1 MTR_5g066490 32.857 140 80 3 89 223 785 915 1.19e-16 79.3
MS.gene022285.t1 MTR_6g079540 33.654 104 69 0 129 232 580 683 4.28e-16 77.4
MS.gene022285.t1 MTR_6g079540 33.654 104 69 0 129 232 828 931 4.62e-16 77.4
MS.gene022285.t1 MTR_3g114900 35.955 89 57 0 129 217 435 523 7.24e-16 76.6
MS.gene022285.t1 MTR_1g099110 37.805 82 48 2 129 209 431 510 1.60e-15 75.9
MS.gene022285.t1 MTR_1g099110 37.805 82 48 2 129 209 448 527 1.86e-15 75.5
MS.gene022285.t1 MTR_1g113960 31.183 93 64 0 129 221 466 558 8.61e-15 73.6
MS.gene022285.t1 MTR_1g113960 31.818 88 60 0 129 216 466 553 1.49e-14 72.8
MS.gene022285.t1 MTR_1g081790 31.250 128 79 2 89 216 826 944 1.88e-14 72.8
MS.gene022285.t1 MTR_1g016300 37.647 85 53 0 129 213 831 915 2.55e-14 72.4
MS.gene022285.t1 MTR_8g442400 34.409 93 59 1 129 219 1563 1655 2.89e-14 72.4
MS.gene022285.t1 MTR_4g116520 33.333 93 60 1 129 219 1269 1361 4.11e-14 72.0
MS.gene022285.t1 MTR_1g050690 31.008 129 80 2 88 216 555 674 4.72e-14 71.6
MS.gene022285.t1 MTR_2g461180 33.333 90 58 1 129 216 1304 1393 6.01e-14 71.2
MS.gene022285.t1 MTR_6g033130 33.333 96 62 1 129 222 1144 1239 7.40e-14 71.2
MS.gene022285.t1 MTR_8g107000 31.818 88 60 0 129 216 897 984 8.46e-14 70.9
MS.gene022285.t1 MTR_7g072710 38.462 78 48 0 136 213 229 306 1.86e-13 69.3
MS.gene022285.t1 MTR_7g072710 38.462 78 48 0 136 213 192 269 2.78e-13 68.6
MS.gene022285.t1 MTR_5g010730 32.955 88 59 0 129 216 591 678 2.99e-13 69.3
MS.gene022285.t1 MTR_3g465590 36.986 73 46 0 141 213 508 580 3.02e-13 68.9
MS.gene022285.t1 MTR_4g128650 34.409 93 59 1 129 219 988 1080 4.38e-13 68.9
MS.gene022285.t1 MTR_5g010730 32.955 88 59 0 129 216 646 733 5.26e-13 68.6
MS.gene022285.t1 MTR_1g061190 29.688 128 81 2 89 216 694 812 1.03e-12 67.8
MS.gene022285.t1 MTR_3g100090 29.457 129 82 2 88 216 791 910 1.13e-12 67.4
MS.gene022285.t1 MTR_1g090020 33.333 93 60 1 129 219 1077 1169 2.28e-12 66.6
MS.gene022285.t1 MTR_2g034020 34.091 88 58 0 129 216 634 721 4.82e-12 65.5
MS.gene022285.t1 MTR_5g072500 32.877 73 49 0 141 213 511 583 1.57e-11 63.9
MS.gene022285.t1 MTR_1g050520 33.333 105 53 2 129 216 211 315 1.59e-11 63.9
MS.gene022285.t1 MTR_4g122160 38.158 76 47 0 129 204 628 703 1.95e-11 63.9
MS.gene022285.t1 MTR_3g104900 27.941 136 83 4 86 215 314 440 2.06e-11 63.5
MS.gene022285.t1 MTR_1g081790 33.333 108 63 2 89 196 826 924 3.95e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022285.t1 AT3G01490 88.596 114 13 0 129 242 298 411 2.59e-74 231
MS.gene022285.t1 AT3G01490 50.299 167 43 3 7 133 12 178 2.69e-46 159
MS.gene022285.t1 AT5G50000 85.965 114 16 0 129 242 272 385 9.97e-74 229
MS.gene022285.t1 AT5G50000 56.209 153 48 2 1 134 1 153 3.64e-52 174
MS.gene022285.t1 AT3G22750 73.684 114 30 0 129 242 265 378 5.79e-65 206
MS.gene022285.t1 AT3G22750 50.382 131 54 1 4 134 26 145 4.60e-39 139
MS.gene022285.t1 AT3G63260 76.316 114 27 0 129 242 278 391 4.76e-63 202
MS.gene022285.t1 AT3G63260 48.031 127 58 2 8 134 36 154 1.46e-30 117
MS.gene022285.t1 AT4G14780 72.807 114 31 0 129 242 251 364 6.18e-63 201
MS.gene022285.t1 AT4G14780 51.181 127 51 2 8 134 17 132 2.88e-35 129
MS.gene022285.t1 AT5G50000 56.209 153 48 2 1 134 1 153 7.35e-53 174
MS.gene022285.t1 AT5G50000 93.478 46 3 0 129 174 272 317 2.32e-26 104
MS.gene022285.t1 AT3G01490 50.909 165 41 3 7 131 12 176 8.00e-47 159
MS.gene022285.t1 AT3G01490 93.478 46 3 0 129 174 298 343 1.28e-25 103
MS.gene022285.t1 AT3G63260 48.031 127 58 2 8 134 36 154 6.48e-31 117
MS.gene022285.t1 AT3G63260 76.087 46 11 0 129 174 278 323 8.54e-20 87.0
MS.gene022285.t1 AT3G46930 42.391 92 52 1 129 219 335 426 7.91e-21 90.9
MS.gene022285.t1 AT3G46930 42.391 92 52 1 129 219 370 461 9.48e-21 90.9
MS.gene022285.t1 AT2G24360 32.749 171 93 5 60 218 229 389 2.03e-20 89.4
MS.gene022285.t1 AT1G62400 30.055 183 100 6 59 224 180 351 3.13e-20 89.0
MS.gene022285.t1 AT5G58950 36.522 115 68 2 129 241 379 490 5.17e-20 89.0
MS.gene022285.t1 AT4G31170 33.835 133 78 3 86 218 268 390 2.12e-19 86.7
MS.gene022285.t1 AT4G31170 33.835 133 78 3 86 218 268 390 2.12e-19 86.7
MS.gene022285.t1 AT4G31170 33.835 133 78 3 86 218 268 390 2.12e-19 86.7
MS.gene022285.t1 AT4G31170 33.835 133 78 3 86 218 268 390 2.12e-19 86.7
MS.gene022285.t1 AT4G31170 33.835 133 78 3 86 218 268 390 2.12e-19 86.7
MS.gene022285.t1 AT4G31170 33.835 133 78 3 86 218 268 390 2.12e-19 86.7
MS.gene022285.t1 AT4G31170 33.835 133 78 3 86 218 268 390 2.12e-19 86.7
MS.gene022285.t1 AT2G17700 36.364 88 56 0 129 216 451 538 7.74e-18 82.4
MS.gene022285.t1 AT3G46920 38.043 92 55 1 129 218 983 1074 3.54e-16 77.8
MS.gene022285.t1 AT3G46920 38.043 92 55 1 129 218 1049 1140 3.82e-16 77.8
MS.gene022285.t1 AT3G24715 35.556 90 56 1 129 216 1013 1102 2.37e-15 75.5
MS.gene022285.t1 AT3G24715 35.556 90 56 1 129 216 1013 1102 2.37e-15 75.5
MS.gene022285.t1 AT3G24715 35.556 90 56 1 129 216 1013 1102 2.37e-15 75.5
MS.gene022285.t1 AT1G16270 36.559 93 57 1 129 219 1040 1132 3.53e-15 75.1
MS.gene022285.t1 AT1G16270 36.559 93 57 1 129 219 1040 1132 3.53e-15 75.1
MS.gene022285.t1 AT1G16270 36.559 93 57 1 129 219 1040 1132 3.53e-15 75.1
MS.gene022285.t1 AT4G35780 31.818 88 60 0 129 216 457 544 7.34e-15 73.9
MS.gene022285.t1 AT1G73660 28.986 138 89 2 89 226 885 1013 9.14e-15 73.9
MS.gene022285.t1 AT5G11850 31.783 129 79 2 88 216 745 864 1.02e-14 73.6
MS.gene022285.t1 AT1G18160 27.536 138 91 2 89 226 852 980 1.03e-14 73.6
MS.gene022285.t1 AT1G79570 34.409 93 59 1 129 219 1141 1233 1.86e-14 72.8
MS.gene022285.t1 AT1G79570 34.409 93 59 1 129 219 1141 1233 1.86e-14 72.8
MS.gene022285.t1 AT4G38470 30.682 88 61 0 129 216 455 542 3.02e-14 72.0
MS.gene022285.t1 AT4G23050 30.526 95 66 0 129 223 636 730 3.50e-14 72.0
MS.gene022285.t1 AT4G23050 30.526 95 66 0 129 223 635 729 3.67e-14 72.0
MS.gene022285.t1 AT1G08720 34.831 89 58 0 129 217 837 925 4.23e-14 71.6
MS.gene022285.t1 AT4G38470 30.682 88 61 0 129 216 455 542 4.34e-14 71.6
MS.gene022285.t1 AT4G24480 31.081 148 92 3 88 235 807 944 4.89e-14 71.6
MS.gene022285.t1 AT1G04700 35.556 90 56 1 129 216 943 1032 8.04e-14 70.9
MS.gene022285.t1 AT1G04700 35.556 90 56 1 129 216 943 1032 8.04e-14 70.9
MS.gene022285.t1 AT4G24480 31.783 129 79 2 88 216 807 926 1.52e-13 70.1
MS.gene022285.t1 AT4G24480 31.783 129 79 2 88 216 812 931 1.81e-13 70.1
MS.gene022285.t1 AT5G01850 35.897 78 50 0 136 213 202 279 3.54e-13 68.2
MS.gene022285.t1 AT5G57610 34.444 90 57 1 129 216 958 1047 3.61e-13 68.9
MS.gene022285.t1 AT5G01850 35.897 78 50 0 136 213 226 303 4.11e-13 68.2
MS.gene022285.t1 AT5G03730 30.534 131 82 1 89 219 690 811 8.35e-13 67.8
MS.gene022285.t1 AT5G03730 30.534 131 82 1 89 219 690 811 8.35e-13 67.8
MS.gene022285.t1 AT2G35050 33.735 83 55 0 137 219 1160 1242 1.28e-12 67.4
MS.gene022285.t1 AT2G35050 33.735 83 55 0 137 219 1160 1242 1.28e-12 67.4
MS.gene022285.t1 AT3G50730 29.054 148 90 4 75 213 155 296 1.45e-12 66.6
MS.gene022285.t1 AT2G40860 33.333 105 53 3 129 216 112 216 1.09e-11 64.3
MS.gene022285.t1 AT5G49470 31.818 88 60 0 129 216 372 459 1.47e-11 63.9
MS.gene022285.t1 AT5G49470 31.818 88 60 0 129 216 659 746 1.87e-11 63.9
MS.gene022285.t1 AT2G40860 33.333 105 53 3 129 216 210 314 1.98e-11 63.9
MS.gene022285.t1 AT2G40860 33.333 105 53 3 129 216 210 314 2.15e-11 63.5
MS.gene022285.t1 AT5G49470 31.818 88 60 0 129 216 532 619 2.18e-11 63.5
MS.gene022285.t1 AT2G40860 33.333 105 53 3 129 216 210 314 2.65e-11 63.5
MS.gene022285.t1 AT5G49470 31.818 88 60 0 129 216 720 807 2.66e-11 63.5
MS.gene022285.t1 AT1G18160 29.630 108 67 2 89 196 852 950 2.66e-11 63.5
MS.gene022285.t1 AT5G49470 31.818 88 60 0 129 216 633 720 2.95e-11 63.2
MS.gene022285.t1 AT5G40540 32.609 92 60 2 136 226 113 203 5.61e-11 61.2
MS.gene022285.t1 AT5G40540 32.609 92 60 2 136 226 134 224 6.98e-11 61.2
MS.gene022285.t1 AT5G40540 32.609 92 60 2 136 226 210 300 7.24e-11 61.6

Find 67 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACAACATCTTGGTATTTC+TGG 0.123520 3.3:-66281191 None:intergenic
ACATCACATTTCCTGTTATA+GGG 0.216565 3.3:-66280981 None:intergenic
TCCATTTCAGGTCTCTTATC+AGG 0.236260 3.3:-66281257 None:intergenic
GCAACTACCTCATCCATTTC+AGG 0.266967 3.3:-66281269 None:intergenic
TTTCTCAAGTTGTTCATCTA+TGG 0.275266 3.3:-66279055 None:intergenic
TACATCACATTTCCTGTTAT+AGG 0.289380 3.3:-66280982 None:intergenic
GTCTTAGCTCGTGGCACTTT+TGG 0.316027 3.3:+66279204 MS.gene022285:CDS
AAATGTGATGTATATAGTTT+TGG 0.339439 3.3:+66280993 MS.gene022285:CDS
ATTACATTTGCCACGGAACT+TGG 0.347319 3.3:-66281212 None:intergenic
TAAATGGCAACCCCTATAAC+AGG 0.362847 3.3:+66280970 MS.gene022285:CDS
CAACCTTGCTGCTGATCAAA+AGG 0.378741 3.3:-66281335 None:intergenic
TACTTCAGCCGTTGTTCGCC+AGG 0.379074 3.3:+66281079 MS.gene022285:CDS
ACTCTTGATGGTGAGGTTAG+AGG 0.382519 3.3:-66279181 None:intergenic
TCACGCAAGAAGTCATTGTT+TGG 0.402517 3.3:+66280462 MS.gene022285:CDS
AAATGTACCTTAGTTACATT+AGG 0.412990 3.3:-66280500 None:intergenic
TCCAGTTAAAATGCTGGATT+GGG 0.421754 3.3:+66280388 MS.gene022285:intron
CGGACAGAAGCTGAAATTGC+TGG 0.422929 3.3:+66280425 MS.gene022285:CDS
TTCAGGTCTCTTATCAGGAT+TGG 0.429223 3.3:-66281252 None:intergenic
AGGGATCATACCTCCACCTT+TGG 0.430939 3.3:-66281315 None:intergenic
TCCTGATAAGAGACCTGAAA+TGG 0.434498 3.3:+66281256 MS.gene022285:CDS
GTCCAGTTAAAATGCTGGAT+TGG 0.434644 3.3:+66280387 MS.gene022285:intron
CTCTTGATGGTGAGGTTAGA+GGG 0.437518 3.3:-66279180 None:intergenic
GAGAAACTTAGGTCAGGATA+TGG 0.443369 3.3:-66281050 None:intergenic
TTAATGTCCAGTTAAAATGC+TGG 0.452338 3.3:+66280382 MS.gene022285:intron
TAATCATCCTAATGTAACTA+AGG 0.467268 3.3:+66280493 MS.gene022285:CDS
ATTGGGGTGAAGAAGGTTAC+CGG 0.469004 3.3:+66280405 MS.gene022285:CDS
CAAATGTAATGAAGAAATGC+TGG 0.471222 3.3:+66281225 MS.gene022285:CDS
ACGGAACTTGGACAACATCT+TGG 0.472680 3.3:-66281200 None:intergenic
GTAAGTTACCAGCAACATCT+TGG 0.474494 3.3:-66279254 None:intergenic
ACGAGCTAAGACACTCTTGA+TGG 0.475212 3.3:-66279193 None:intergenic
ATGCTGGATTGGGGTGAAGA+AGG 0.482593 3.3:+66280398 MS.gene022285:CDS
AAGTAATCTCTGAGAAACTT+AGG 0.484597 3.3:-66281061 None:intergenic
TATGATGGCCAAGATGTTGC+TGG 0.488459 3.3:+66279246 MS.gene022285:CDS
ATCTCTGAGAAACTTAGGTC+AGG 0.495056 3.3:-66281056 None:intergenic
CAGCAAGGTTGTCTCTGCTT+CGG 0.497717 3.3:+66281347 MS.gene022285:CDS
AAAATGATGGAAAAGAATGA+TGG 0.498117 3.3:+66279000 None:intergenic
CAGCAATTTCAGCTTCTGTC+CGG 0.499893 3.3:-66280424 None:intergenic
TCTGCTTCGGTAAGCGCCGA+GGG 0.505983 3.3:+66281360 MS.gene022285:CDS
GTTCACCGTGGTTTCTATGA+TGG 0.507375 3.3:+66279231 MS.gene022285:CDS
CATCACATTTCCTGTTATAG+GGG 0.508040 3.3:-66280980 None:intergenic
GGATGAGGTAGTTGCCATGC+TGG 0.516875 3.3:+66281277 MS.gene022285:CDS
TCAAACTCTATTGCTACTAC+TGG 0.530513 3.3:+66279117 MS.gene022285:CDS
AAATGATGGAAAAGAATGAT+GGG 0.533794 3.3:+66279001 None:intergenic
AAGCTGAAATTGCTGGACTC+AGG 0.535768 3.3:+66280432 MS.gene022285:CDS
AACCTTGCTGCTGATCAAAA+GGG 0.536519 3.3:-66281334 None:intergenic
CATGGAGCAAAGAGAAGAGT+GGG 0.541395 3.3:+66279149 MS.gene022285:CDS
CTCTGCTTCGGTAAGCGCCG+AGG 0.551514 3.3:+66281359 MS.gene022285:CDS
AACTCTATTGCTACTACTGG+TGG 0.569622 3.3:+66279120 MS.gene022285:CDS
TAAGAGACCTGAAATGGATG+AGG 0.598450 3.3:+66281262 MS.gene022285:CDS
CTAAGACACTCTTGATGGTG+AGG 0.606744 3.3:-66279188 None:intergenic
ACATGGAGCAAAGAGAAGAG+TGG 0.607383 3.3:+66279148 MS.gene022285:CDS
TGACTGTAAATCAAGGCCCT+CGG 0.612579 3.3:-66281376 None:intergenic
ATCAAGAGTGTCTTAGCTCG+TGG 0.616920 3.3:+66279195 MS.gene022285:CDS
AAGATATACCTGGCGAACAA+CGG 0.618092 3.3:-66281087 None:intergenic
ATTGACACATCCAAAGGTGG+AGG 0.618735 3.3:+66281305 MS.gene022285:CDS
AAATGTAATGAAGAAATGCT+GGG 0.631816 3.3:+66281226 MS.gene022285:CDS
TTTCTTCATTACATTTGCCA+CGG 0.633341 3.3:-66281219 None:intergenic
GCTATTGACACATCCAAAGG+TGG 0.643411 3.3:+66281302 MS.gene022285:CDS
TCTTGATGGTGAGGTTAGAG+GGG 0.645628 3.3:-66279179 None:intergenic
TACTACTGGTGGTAATAACA+TGG 0.654878 3.3:+66279131 MS.gene022285:CDS
AAGATGTTGTCCAAGTTCCG+TGG 0.660139 3.3:+66281202 MS.gene022285:CDS
GAGGCTATTGACACATCCAA+AGG 0.661527 3.3:+66281299 MS.gene022285:CDS
TGTGTCAATAGCCTCCAGCA+TGG 0.665984 3.3:-66281291 None:intergenic
CTTGGCCATCATAGAAACCA+CGG 0.693286 3.3:-66279236 None:intergenic
TGAGGTAGTTGCCATGCTGG+AGG 0.694334 3.3:+66281280 MS.gene022285:CDS
ACTTGAGAAACACTTCAACA+AGG 0.709526 3.3:+66279068 MS.gene022285:CDS
CCAGTTAAAATGCTGGATTG+GGG 0.753014 3.3:+66280389 MS.gene022285:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAAGTTGTTAAAATAC+AGG + chr3.3:66279522-66279541 MS.gene022285:intron 15.0%
!!! AAAAATTTGAAGTAACTATT+TGG - chr3.3:66279767-66279786 None:intergenic 15.0%
!!! TTTTAACTTATTTGACAAAT+GGG + chr3.3:66280926-66280945 MS.gene022285:intron 15.0%
!! ATACAATTGAAACTAATCTA+TGG - chr3.3:66281116-66281135 None:intergenic 20.0%
!! ATGAATCCATATTGATATAT+TGG + chr3.3:66281141-66281160 MS.gene022285:intron 20.0%
!! TACAAACCAATATATCAATA+TGG - chr3.3:66281150-66281169 None:intergenic 20.0%
!! TCAAATAAGTCAATTCAAAT+AGG + chr3.3:66280143-66280162 MS.gene022285:intron 20.0%
!! TTAACAACTTATTATCAATC+TGG - chr3.3:66279517-66279536 None:intergenic 20.0%
!!! AAATGTGATGTATATAGTTT+TGG + chr3.3:66280993-66281012 MS.gene022285:CDS 20.0%
!!! ACAATAACACTTAACTTTTT+TGG + chr3.3:66280349-66280368 MS.gene022285:intron 20.0%
!!! ATCAAATAAAAGCTTTTTTG+TGG - chr3.3:66280881-66280900 None:intergenic 20.0%
!!! CAATAACACTTAACTTTTTT+GGG + chr3.3:66280350-66280369 MS.gene022285:intron 20.0%
!!! CTTTTAACTTATTTGACAAA+TGG + chr3.3:66280925-66280944 MS.gene022285:intron 20.0%
! AAAGTCAAAGACTAATTTAG+TGG + chr3.3:66279647-66279666 MS.gene022285:intron 25.0%
! AAATGTAATGAAGAAATGCT+GGG + chr3.3:66281226-66281245 MS.gene022285:CDS 25.0%
! AAATGTACCTTAGTTACATT+AGG - chr3.3:66280503-66280522 None:intergenic 25.0%
! CTAATTTCAATAATCTCTCT+AGG - chr3.3:66280039-66280058 None:intergenic 25.0%
! CTAGAGAGATTATTGAAATT+AGG + chr3.3:66280037-66280056 MS.gene022285:intron 25.0%
! TAATCATCCTAATGTAACTA+AGG + chr3.3:66280493-66280512 MS.gene022285:CDS 25.0%
!! AATTAGGTGAAAATAGCTTT+TGG + chr3.3:66280053-66280072 MS.gene022285:intron 25.0%
!! ATTAGAAGATTGGAATGTTT+TGG + chr3.3:66279900-66279919 MS.gene022285:intron 25.0%
!! ATTTTATGGTAAAGGTTTCA+AGG - chr3.3:66280832-66280851 None:intergenic 25.0%
!! CTTTACAATGATTTGATGAT+GGG + chr3.3:66279844-66279863 MS.gene022285:intron 25.0%
!! TTTTATGGTAAAGGTTTCAA+GGG - chr3.3:66280831-66280850 None:intergenic 25.0%
!!! AAATTAGTCTTTGACTTTGT+AGG - chr3.3:66279645-66279664 None:intergenic 25.0%
!!! AATCATTTTGAGAGTATACT+AGG + chr3.3:66279459-66279478 MS.gene022285:intron 25.0%
!!! ATAGTTTTGGCATATGTTTA+TGG + chr3.3:66281006-66281025 MS.gene022285:CDS 25.0%
!!! ATAGTTTTGTTTTCAGGAAA+CGG + chr3.3:66280631-66280650 MS.gene022285:intron 25.0%
!!! TAGTTTTGGCATATGTTTAT+GGG + chr3.3:66281007-66281026 MS.gene022285:CDS 25.0%
!!! TATCTACTTATTGCTGATTT+CGG + chr3.3:66280714-66280733 MS.gene022285:intron 25.0%
!!! TGATCGATTTTAATTTTGTG+AGG + chr3.3:66280284-66280303 MS.gene022285:intron 25.0%
AAATGTAGGGATCAACTTAA+TGG + chr3.3:66279565-66279584 MS.gene022285:intron 30.0%
AACCAACAAGATAACATCAA+CGG - chr3.3:66279687-66279706 None:intergenic 30.0%
ACATCACATTTCCTGTTATA+GGG - chr3.3:66280984-66281003 None:intergenic 30.0%
ATTTAACCGGTTACTCAATT+AGG - chr3.3:66279549-66279568 None:intergenic 30.0%
CAAATGTAATGAAGAAATGC+TGG + chr3.3:66281225-66281244 MS.gene022285:CDS 30.0%
CCATCATCAAATCATTGTAA+AGG - chr3.3:66279846-66279865 None:intergenic 30.0%
CCTTTACAATGATTTGATGA+TGG + chr3.3:66279843-66279862 MS.gene022285:intron 30.0%
GTAGCATGTTATTGTTAGAT+TGG + chr3.3:66280190-66280209 MS.gene022285:intron 30.0%
TACATCACATTTCCTGTTAT+AGG - chr3.3:66280985-66281004 None:intergenic 30.0%
TGAAAATGTGTTATCCAAGT+TGG + chr3.3:66279927-66279946 MS.gene022285:intron 30.0%
TTAATGTCCAGTTAAAATGC+TGG + chr3.3:66280382-66280401 MS.gene022285:intron 30.0%
TTTCTTCATTACATTTGCCA+CGG - chr3.3:66281222-66281241 None:intergenic 30.0%
! AAGTAATCTCTGAGAAACTT+AGG - chr3.3:66281064-66281083 None:intergenic 30.0%
! AGCGAAATTGTTTCTTTCAT+CGG - chr3.3:66280265-66280284 None:intergenic 30.0%
! CATATTGGCGCAATATTTTA+TGG - chr3.3:66280846-66280865 None:intergenic 30.0%
! TGTTATTGTTAGATTGGTTG+TGG + chr3.3:66280196-66280215 MS.gene022285:intron 30.0%
! TTTCTCAAGTTGTTCATCTA+TGG - chr3.3:66279058-66279077 None:intergenic 30.0%
!!! GATGGTTTACAGGTTTTAAA+TGG + chr3.3:66280954-66280973 MS.gene022285:intron 30.0%
AACCGTTGATGTTATCTTGT+TGG + chr3.3:66279682-66279701 MS.gene022285:intron 35.0%
AATCTTCTAATGAGAAGCAG+AGG - chr3.3:66279891-66279910 None:intergenic 35.0%
AATTCAAATAGGCTCTTGTG+TGG + chr3.3:66280154-66280173 MS.gene022285:intron 35.0%
ACTTGAGAAACACTTCAACA+AGG + chr3.3:66279068-66279087 MS.gene022285:CDS 35.0%
AGAGAGTTGAAGCTAATGAA+AGG - chr3.3:66280526-66280545 None:intergenic 35.0%
AGTTGATCCCTACATTTAAC+CGG - chr3.3:66279562-66279581 None:intergenic 35.0%
ATCTTCTAATGAGAAGCAGA+GGG - chr3.3:66279890-66279909 None:intergenic 35.0%
CATCACATTTCCTGTTATAG+GGG - chr3.3:66280983-66281002 None:intergenic 35.0%
CGAATCCTAATGATATGACT+GGG + chr3.3:66280757-66280776 MS.gene022285:intron 35.0%
GAAGGGGACAAAAATTTAGA+TGG + chr3.3:66280218-66280237 MS.gene022285:intron 35.0%
GAATCCTAATGATATGACTG+GGG + chr3.3:66280758-66280777 MS.gene022285:intron 35.0%
GCGCCAATATGAAAAGAAAA+GGG + chr3.3:66280855-66280874 MS.gene022285:intron 35.0%
TACTACTGGTGGTAATAACA+TGG + chr3.3:66279131-66279150 MS.gene022285:CDS 35.0%
TCAAACTCTATTGCTACTAC+TGG + chr3.3:66279117-66279136 MS.gene022285:CDS 35.0%
TCCAGTTAAAATGCTGGATT+GGG + chr3.3:66280388-66280407 MS.gene022285:intron 35.0%
TCGAATCCTAATGATATGAC+TGG + chr3.3:66280756-66280775 MS.gene022285:intron 35.0%
TCTATGGAAGAAGATATACC+TGG - chr3.3:66281100-66281119 None:intergenic 35.0%
TCTGCTTCTCATTAGAAGAT+TGG + chr3.3:66279890-66279909 MS.gene022285:intron 35.0%
TGAGTAACCGGTTAAATGTA+GGG + chr3.3:66279552-66279571 MS.gene022285:intron 35.0%
TGCGCCAATATGAAAAGAAA+AGG + chr3.3:66280854-66280873 MS.gene022285:intron 35.0%
TTGAGTAACCGGTTAAATGT+AGG + chr3.3:66279551-66279570 MS.gene022285:intron 35.0%
TTTGACAAATGGGTGAATGA+TGG + chr3.3:66280936-66280955 MS.gene022285:intron 35.0%
! ATTGTTAGATTGGTTGTGGA+AGG + chr3.3:66280200-66280219 MS.gene022285:intron 35.0%
! ATTGTTTCTTTCATCGGTCT+TGG - chr3.3:66280259-66280278 None:intergenic 35.0%
! GGCGCAATATTTTATGGTAA+AGG - chr3.3:66280840-66280859 None:intergenic 35.0%
! TGGCCCTTTTCTTTTCATAT+TGG - chr3.3:66280861-66280880 None:intergenic 35.0%
! TTGTTAGATTGGTTGTGGAA+GGG + chr3.3:66280201-66280220 MS.gene022285:intron 35.0%
!!! CGAGTGATAGTTTTGTTTTC+AGG + chr3.3:66280625-66280644 MS.gene022285:intron 35.0%
AACCTTGCTGCTGATCAAAA+GGG - chr3.3:66281337-66281356 None:intergenic 40.0%
AACTCTATTGCTACTACTGG+TGG + chr3.3:66279120-66279139 MS.gene022285:CDS 40.0%
AAGATATACCTGGCGAACAA+CGG - chr3.3:66281090-66281109 None:intergenic 40.0%
ACAGGACCTAATTGAGTAAC+CGG + chr3.3:66279540-66279559 MS.gene022285:intron 40.0%
ATGGGTGAATGATGGTTTAC+AGG + chr3.3:66280944-66280963 MS.gene022285:intron 40.0%
ATTACATTTGCCACGGAACT+TGG - chr3.3:66281215-66281234 None:intergenic 40.0%
CCAGTTAAAATGCTGGATTG+GGG + chr3.3:66280389-66280408 MS.gene022285:intron 40.0%
GAGAAACTTAGGTCAGGATA+TGG - chr3.3:66281053-66281072 None:intergenic 40.0%
GGACAACATCTTGGTATTTC+TGG - chr3.3:66281194-66281213 None:intergenic 40.0%
GTAAAGGTTTCAAGGGAAAC+AGG - chr3.3:66280824-66280843 None:intergenic 40.0%
GTAAGTTACCAGCAACATCT+TGG - chr3.3:66279257-66279276 None:intergenic 40.0%
GTCCAGTTAAAATGCTGGAT+TGG + chr3.3:66280387-66280406 MS.gene022285:intron 40.0%
TAAATGGCAACCCCTATAAC+AGG + chr3.3:66280970-66280989 MS.gene022285:CDS 40.0%
TAAGAGACCTGAAATGGATG+AGG + chr3.3:66281262-66281281 MS.gene022285:CDS 40.0%
TACAAAGTAGGGGACGAATT+TGG - chr3.3:66279363-66279382 None:intergenic 40.0%
TACTAGGAATTGACGCTAAG+TGG + chr3.3:66279475-66279494 MS.gene022285:intron 40.0%
TCACGCAAGAAGTCATTGTT+TGG + chr3.3:66280462-66280481 MS.gene022285:CDS 40.0%
TCCTGATAAGAGACCTGAAA+TGG + chr3.3:66281256-66281275 MS.gene022285:CDS 40.0%
TTCAGGTCTCTTATCAGGAT+TGG - chr3.3:66281255-66281274 None:intergenic 40.0%
! ATCTCTGAGAAACTTAGGTC+AGG - chr3.3:66281059-66281078 None:intergenic 40.0%
! GTAGAGCGTCTTACAAAGTA+GGG - chr3.3:66279374-66279393 None:intergenic 40.0%
! TAGAGCGTCTTACAAAGTAG+GGG - chr3.3:66279373-66279392 None:intergenic 40.0%
! TCCATTTCAGGTCTCTTATC+AGG - chr3.3:66281260-66281279 None:intergenic 40.0%
! TGTAGAGCGTCTTACAAAGT+AGG - chr3.3:66279375-66279394 None:intergenic 40.0%
! TGTTAGATTGGTTGTGGAAG+GGG + chr3.3:66280202-66280221 MS.gene022285:intron 40.0%
!!! GATTTGATGATGGGCTGTTT+TGG + chr3.3:66279853-66279872 MS.gene022285:intron 40.0%
AAAGTAGGGGACGAATTTGG+CGG - chr3.3:66279360-66279379 None:intergenic 45.0%
AAGATGTTGTCCAAGTTCCG+TGG + chr3.3:66281202-66281221 MS.gene022285:CDS 45.0%
AAGCTGAAATTGCTGGACTC+AGG + chr3.3:66280432-66280451 MS.gene022285:CDS 45.0%
AATGATATGACTGGGGAGAC+TGG + chr3.3:66280765-66280784 MS.gene022285:intron 45.0%
ACATGGAGCAAAGAGAAGAG+TGG + chr3.3:66279148-66279167 MS.gene022285:CDS 45.0%
ACGAGCTAAGACACTCTTGA+TGG - chr3.3:66279196-66279215 None:intergenic 45.0%
ACGGAACTTGGACAACATCT+TGG - chr3.3:66281203-66281222 None:intergenic 45.0%
ATTGACACATCCAAAGGTGG+AGG + chr3.3:66281305-66281324 MS.gene022285:CDS 45.0%
ATTGGGGTGAAGAAGGTTAC+CGG + chr3.3:66280405-66280424 MS.gene022285:CDS 45.0%
CAACCTTGCTGCTGATCAAA+AGG - chr3.3:66281338-66281357 None:intergenic 45.0%
CAGCAATTTCAGCTTCTGTC+CGG - chr3.3:66280427-66280446 None:intergenic 45.0%
CATGGAGCAAAGAGAAGAGT+GGG + chr3.3:66279149-66279168 MS.gene022285:CDS 45.0%
CTTGGCCATCATAGAAACCA+CGG - chr3.3:66279239-66279258 None:intergenic 45.0%
GACGAATTTGGCGGTTTAGA+CGG - chr3.3:66279351-66279370 None:intergenic 45.0%
GACTGGTACACTTGGTTACA+TGG + chr3.3:66280782-66280801 MS.gene022285:intron 45.0%
GAGGCTATTGACACATCCAA+AGG + chr3.3:66281299-66281318 MS.gene022285:CDS 45.0%
GCAACTACCTCATCCATTTC+AGG - chr3.3:66281272-66281291 None:intergenic 45.0%
GCTATTGACACATCCAAAGG+TGG + chr3.3:66281302-66281321 MS.gene022285:CDS 45.0%
GTCTCCCCAGTCATATCATT+AGG - chr3.3:66280765-66280784 None:intergenic 45.0%
GTTCACCGTGGTTTCTATGA+TGG + chr3.3:66279231-66279250 MS.gene022285:CDS 45.0%
TATGATGGCCAAGATGTTGC+TGG + chr3.3:66279246-66279265 MS.gene022285:CDS 45.0%
TTCCATAAGCTCTCTCAGAG+AGG + chr3.3:66280092-66280111 MS.gene022285:intron 45.0%
! ACTTTTGGTACTGTTCACCG+TGG + chr3.3:66279219-66279238 MS.gene022285:CDS 45.0%
! ATCCCTTTTGATCAGCAGCA+AGG + chr3.3:66281332-66281351 MS.gene022285:CDS 45.0%
! TAAGCTGATTAGCCCAACCA+GGG - chr3.3:66279962-66279981 None:intergenic 45.0%
! TTAAGCTGATTAGCCCAACC+AGG - chr3.3:66279963-66279982 None:intergenic 45.0%
!! ACTCTTGATGGTGAGGTTAG+AGG - chr3.3:66279184-66279203 None:intergenic 45.0%
!! ATCAAGAGTGTCTTAGCTCG+TGG + chr3.3:66279195-66279214 MS.gene022285:CDS 45.0%
!! CCCCAATCCAGCATTTTAAC+TGG - chr3.3:66280392-66280411 None:intergenic 45.0%
!! CTAAGACACTCTTGATGGTG+AGG - chr3.3:66279191-66279210 None:intergenic 45.0%
!! CTCTTGATGGTGAGGTTAGA+GGG - chr3.3:66279183-66279202 None:intergenic 45.0%
!! TCTTGATGGTGAGGTTAGAG+GGG - chr3.3:66279182-66279201 None:intergenic 45.0%
ACTTGGTTACATGGCTCCTG+AGG + chr3.3:66280791-66280810 MS.gene022285:intron 50.0%
AGGGATCATACCTCCACCTT+TGG - chr3.3:66281318-66281337 None:intergenic 50.0%
CAGCAAGGTTGTCTCTGCTT+CGG + chr3.3:66281347-66281366 MS.gene022285:CDS 50.0%
CAGGGCAAACAACACCAACT+TGG - chr3.3:66279944-66279963 None:intergenic 50.0%
CGGACAGAAGCTGAAATTGC+TGG + chr3.3:66280425-66280444 MS.gene022285:CDS 50.0%
GGAAACAGGCTACAAACCTC+AGG - chr3.3:66280810-66280829 None:intergenic 50.0%
TGTGTCAATAGCCTCCAGCA+TGG - chr3.3:66281294-66281313 None:intergenic 50.0%
! AGCCTCTCTGAGAGAGCTTA+TGG - chr3.3:66280097-66280116 None:intergenic 50.0%
! ATGCTGGATTGGGGTGAAGA+AGG + chr3.3:66280398-66280417 MS.gene022285:CDS 50.0%
! GTCTTAGCTCGTGGCACTTT+TGG + chr3.3:66279204-66279223 MS.gene022285:CDS 50.0%
!! CAAGTTGGTGTTGTTTGCCC+TGG + chr3.3:66279942-66279961 MS.gene022285:intron 50.0%
!! TGGTGTTGTTTGCCCTGGTT+GGG + chr3.3:66279947-66279966 MS.gene022285:intron 50.0%
!! TTGGTGTTGTTTGCCCTGGT+TGG + chr3.3:66279946-66279965 MS.gene022285:intron 50.0%
ACTGGGGAGACTGGTACACT+TGG + chr3.3:66280774-66280793 MS.gene022285:intron 55.0%
GGATGAGGTAGTTGCCATGC+TGG + chr3.3:66281277-66281296 MS.gene022285:CDS 55.0%
TACTTCAGCCGTTGTTCGCC+AGG + chr3.3:66281079-66281098 MS.gene022285:CDS 55.0%
TGAGGTAGTTGCCATGCTGG+AGG + chr3.3:66281280-66281299 MS.gene022285:CDS 55.0%
!! TCTGCTTCGGTAAGCGCCGA+GGG + chr3.3:66281360-66281379 MS.gene022285:CDS 60.0%
! CTCTGCTTCGGTAAGCGCCG+AGG + chr3.3:66281359-66281378 MS.gene022285:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 66279003 66281388 66279003 ID=MS.gene022285
chr3.3 mRNA 66279003 66281388 66279003 ID=MS.gene022285.t1;Parent=MS.gene022285
chr3.3 exon 66279003 66279267 66279003 ID=MS.gene022285.t1.exon1;Parent=MS.gene022285.t1
chr3.3 CDS 66279003 66279267 66279003 ID=cds.MS.gene022285.t1;Parent=MS.gene022285.t1
chr3.3 exon 66280393 66280514 66280393 ID=MS.gene022285.t1.exon2;Parent=MS.gene022285.t1
chr3.3 CDS 66280393 66280514 66280393 ID=cds.MS.gene022285.t1;Parent=MS.gene022285.t1
chr3.3 exon 66280966 66281100 66280966 ID=MS.gene022285.t1.exon3;Parent=MS.gene022285.t1
chr3.3 CDS 66280966 66281100 66280966 ID=cds.MS.gene022285.t1;Parent=MS.gene022285.t1
chr3.3 exon 66281182 66281388 66281182 ID=MS.gene022285.t1.exon4;Parent=MS.gene022285.t1
chr3.3 CDS 66281182 66281388 66281182 ID=cds.MS.gene022285.t1;Parent=MS.gene022285.t1
Gene Sequence

>MS.gene022285

ATGATGGAAAAGAATGATGGGTTTGTGAGAGCTGATCAAATTGATCTAAAGACCATAGATGAACAACTTGAGAAACACTTCAACAAGGTAAATGATGATTCTTCTCATGATCATTCAAACTCTATTGCTACTACTGGTGGTAATAACATGGAGCAAAGAGAAGAGTGGGAAATTGACCCCTCTAACCTCACCATCAAGAGTGTCTTAGCTCGTGGCACTTTTGGTACTGTTCACCGTGGTTTCTATGATGGCCAAGATGTTGCTGGTAACTTACATAAGTTTCTTTTCAAAATTTCATTTTTTTATTTGTTTCATGTTTCTATTTTTTTACACTCACTCTTCAAACCGTCTAAACCGCCAAATTCGTCCCCTACTTTGTAAGACGCTCTACAATAGTTTCATGATATTATGAATATTTTAAAAAGTTTTATTACTCTGTCAAAATTTACTCGTATTAATCATTTTGAGAGTATACTAGGAATTGACGCTAAGTGGTTAAATAGTATAGAGACCAGATTGATAATAAGTTGTTAAAATACAGGACCTAATTGAGTAACCGGTTAAATGTAGGGATCAACTTAATGGTTTAATAGAGTCAGAGATCTTATATTCATAATTTGAGAACGTGTTTAAGAGTGTCCTACAAAGTCAAAGACTAATTTAGTGGTTAACTCGAACTAACCGTTGATGTTATCTTGTTGGTTCACTTAAGAATCATTGAGTCAATACATCTAATTATCATAATTCGTTGTTGATTTTATCCAAATAGTTACTTCAAATTTTTCTCAGTTCAATTCATTGAGAGAGCTGTGTATTTTTATTTTCTTTAAAAAGAATCGACCTTTACAATGATTTGATGATGGGCTGTTTTGGAAATTACTGTGCCCTCTGCTTCTCATTAGAAGATTGGAATGTTTTGGCATCTGAAAATGTGTTATCCAAGTTGGTGTTGTTTGCCCTGGTTGGGCTAATCAGCTTAATTAGACTCTTATAGCATAAGCATTTATTATGTAAGTGTTTTTGTATAATTTATCCTAGAGAGATTATTGAAATTAGGTGAAAATAGCTTTTGGACACGTCACAACTTGTTTCCATAAGCTCTCTCAGAGAGGCTCAAAGTGTTTACGCTAGTAGATATGCTCAAATAAGTCAATTCAAATAGGCTCTTGTGTGGTTACTGAATACAAGTAGCATGTTATTGTTAGATTGGTTGTGGAAGGGGACAAAAATTTAGATGGACAGAGAATCACAGACCAAGACCGATGAAAGAAACAATTTCGCTGATCGATTTTAATTTTGTGAGGATTCTATAAATCTAAAATGCACTTTACTGTGCATATAATGTAACAATAACACTTAACTTTTTTGGGCTTATTTACTTAATGTCCAGTTAAAATGCTGGATTGGGGTGAAGAAGGTTACCGGACAGAAGCTGAAATTGCTGGACTCAGGTCTGCATTCACGCAAGAAGTCATTGTTTGGCATAAACTTAATCATCCTAATGTAACTAAGGTACATTTCCTTTCATTAGCTTCAACTCTCTCTTGTTTCTGTTGTCAATTGTTCTTGTAGATTACAATATTGCATATCAAAAGTGTGAGATCATACAAAACTTGCATTATCGAGTGATAGTTTTGTTTTCAGGAAACGGTTATTCACTAGTCGACTATGACAACTTGCTTTCAGTTTTAAGTAACACACTGAACACTTTTATCTACTTATTGCTGATTTCGGTGTTGCACGTATTGAAGCGTCGAATCCTAATGATATGACTGGGGAGACTGGTACACTTGGTTACATGGCTCCTGAGGTTTGTAGCCTGTTTCCCTTGAAACCTTTACCATAAAATATTGCGCCAATATGAAAAGAAAAGGGCCACAAAAAAGCTTTTATTTGATAAAAGAAAAAGTCTAAGCAATTTACTTTTAACTTATTTGACAAATGGGTGAATGATGGTTTACAGGTTTTAAATGGCAACCCCTATAACAGGAAATGTGATGTATATAGTTTTGGCATATGTTTATGGGAAATATATTGTTGTGACATGCCATATCCTGACCTAAGTTTCTCAGAGATTACTTCAGCCGTTGTTCGCCAGGTATATCTTCTTCCATAGATTAGTTTCAATTGTATCTAAAATGAATCCATATTGATATATTGGTTTGTATTGAATTTTTAGAATGTAAGACCAGAAATACCAAGATGTTGTCCAAGTTCCGTGGCAAATGTAATGAAGAAATGCTGGGATGCCAATCCTGATAAGAGACCTGAAATGGATGAGGTAGTTGCCATGCTGGAGGCTATTGACACATCCAAAGGTGGAGGTATGATCCCTTTTGATCAGCAGCAAGGTTGTCTCTGCTTCGGTAAGCGCCGAGGGCCTTGA
Protein sequence

>MS.gene022285.t1

MMEKNDGFVRADQIDLKTIDEQLEKHFNKVNDDSSHDHSNSIATTGGNNMEQREEWEIDPSNLTIKSVLARGTFGTVHRGFYDGQDVAVKMLDWGEEGYRTEAEIAGLRSAFTQEVIVWHKLNHPNVTKVLNGNPYNRKCDVYSFGICLWEIYCCDMPYPDLSFSEITSAVVRQNVRPEIPRCCPSSVANVMKKCWDANPDKRPEMDEVVAMLEAIDTSKGGGMIPFDQQQGCLCFGKRRGP