AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022302

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022302.t1 MTR_3g077750 98.810 336 4 0 1 336 1 336 0.0 690
MS.gene022302.t1 MTR_3g091820 34.320 338 153 17 17 324 5 303 2.42e-40 144
MS.gene022302.t1 MTR_4g461080 55.039 129 42 3 39 159 9 129 4.43e-36 132
MS.gene022302.t1 MTR_2g093220 54.331 127 45 4 38 155 19 141 1.21e-35 131
MS.gene022302.t1 MTR_8g027295 52.800 125 44 4 45 158 10 130 1.96e-35 130
MS.gene022302.t1 MTR_4g063780 58.491 106 37 3 44 142 55 160 2.96e-34 128
MS.gene022302.t1 MTR_2g096740 72.603 73 18 1 50 122 51 121 8.85e-34 126
MS.gene022302.t1 MTR_2g096740 72.603 73 18 1 50 122 66 136 9.47e-34 126
MS.gene022302.t1 MTR_1g077600 53.125 128 46 5 41 163 25 143 1.58e-33 125
MS.gene022302.t1 MTR_1g077600 53.125 128 46 5 41 163 25 143 1.73e-33 125
MS.gene022302.t1 MTR_8g479350 66.197 71 24 0 61 131 68 138 3.74e-31 120
MS.gene022302.t1 MTR_4g022370 69.333 75 20 2 44 115 62 136 1.27e-30 119
MS.gene022302.t1 MTR_7g059400 60.870 92 29 3 27 115 43 130 5.02e-30 117
MS.gene022302.t1 MTR_7g024670 56.122 98 27 3 34 115 34 131 1.04e-29 117
MS.gene022302.t1 MTR_8g068210 67.164 67 22 0 50 116 21 87 1.08e-29 116
MS.gene022302.t1 MTR_3g090430 71.875 64 18 0 52 115 42 105 1.09e-29 116
MS.gene022302.t1 MTR_2g014060 77.049 61 14 0 52 112 16 76 1.72e-29 115
MS.gene022302.t1 MTR_4g088580 81.818 55 10 0 62 116 56 110 2.33e-29 116
MS.gene022302.t1 MTR_8g079060 55.319 94 35 2 52 141 16 106 1.44e-28 110
MS.gene022302.t1 MTR_5g031440 78.571 56 12 0 56 111 38 93 1.57e-28 113
MS.gene022302.t1 MTR_1g056810 71.212 66 19 0 59 124 10 75 3.04e-28 111
MS.gene022302.t1 MTR_8g015840 74.603 63 16 0 56 118 15 77 8.44e-28 108
MS.gene022302.t1 MTR_4g109980 84.314 51 8 0 61 111 40 90 7.92e-27 108
MS.gene022302.t1 MTR_2g014170 84.000 50 8 0 60 109 44 93 5.20e-26 105
MS.gene022302.t1 MTR_2g059540 82.353 51 9 0 59 109 29 79 8.76e-26 102
MS.gene022302.t1 MTR_3g435480 66.667 69 22 1 50 118 126 193 1.39e-25 107
MS.gene022302.t1 MTR_2g013370 47.458 118 42 5 1 116 1 100 2.64e-25 103
MS.gene022302.t1 MTR_4g089095 76.786 56 12 1 61 116 16 70 3.04e-25 102
MS.gene022302.t1 MTR_4g089095 76.786 56 12 1 61 116 50 104 3.10e-25 103
MS.gene022302.t1 MTR_7g010950 43.697 119 59 2 25 136 93 210 4.97e-24 102
MS.gene022302.t1 MTR_4g082060 60.870 69 26 1 50 118 112 179 5.73e-24 102
MS.gene022302.t1 MTR_6g012450 44.545 110 54 1 30 132 98 207 7.30e-24 102
MS.gene022302.t1 MTR_7g086780 42.342 111 58 2 20 130 64 168 1.47e-23 100
MS.gene022302.t1 MTR_6g027450 42.478 113 57 4 7 119 43 147 3.12e-23 98.6
MS.gene022302.t1 MTR_8g044220 53.333 75 34 1 41 115 99 172 6.25e-23 99.0
MS.gene022302.t1 MTR_6g027460 55.714 70 30 1 50 119 86 154 9.31e-23 97.8
MS.gene022302.t1 MTR_5g041420 41.600 125 55 3 12 118 71 195 3.65e-22 95.5
MS.gene022302.t1 MTR_2g016030 66.667 54 18 0 61 114 47 100 4.52e-22 91.3
MS.gene022302.t1 MTR_5g041530 63.793 58 21 0 57 114 136 193 1.86e-20 91.7
MS.gene022302.t1 MTR_5g041400 45.161 93 47 1 26 114 80 172 4.14e-20 90.1
MS.gene022302.t1 MTR_5g041380 68.519 54 17 0 61 114 127 180 8.89e-20 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022302.t1 AT5G62940 42.748 393 145 23 1 334 1 372 1.23e-55 186
MS.gene022302.t1 AT5G60200 66.292 89 27 3 33 121 30 115 2.44e-35 129
MS.gene022302.t1 AT1G51700 47.656 128 54 2 56 170 28 155 6.94e-33 121
MS.gene022302.t1 AT2G28510 56.364 110 35 2 63 160 50 158 5.09e-32 121
MS.gene022302.t1 AT3G45610 43.373 166 68 7 54 212 32 178 2.35e-31 118
MS.gene022302.t1 AT4G24060 50.000 122 46 2 50 156 42 163 2.66e-31 120
MS.gene022302.t1 AT5G02460 70.667 75 19 2 44 115 75 149 5.04e-31 121
MS.gene022302.t1 AT3G55370 65.000 80 25 2 43 119 55 134 7.04e-31 119
MS.gene022302.t1 AT3G55370 65.000 80 25 2 43 119 55 134 8.62e-31 119
MS.gene022302.t1 AT3G55370 65.000 80 25 2 43 119 100 179 1.27e-30 119
MS.gene022302.t1 AT2G37590 64.474 76 24 1 44 116 69 144 1.70e-30 118
MS.gene022302.t1 AT1G07640 69.444 72 19 1 47 115 4 75 6.19e-30 115
MS.gene022302.t1 AT5G60850 66.234 77 20 1 50 120 34 110 6.56e-30 116
MS.gene022302.t1 AT1G07640 69.444 72 19 1 47 115 60 131 1.06e-29 116
MS.gene022302.t1 AT1G07640 69.444 72 19 1 47 115 68 139 1.19e-29 116
MS.gene022302.t1 AT1G64620 52.672 131 47 3 45 164 33 159 1.73e-29 116
MS.gene022302.t1 AT3G61850 65.753 73 23 1 46 118 49 119 3.42e-29 114
MS.gene022302.t1 AT3G61850 65.753 73 23 1 46 118 49 119 3.42e-29 114
MS.gene022302.t1 AT5G65590 62.963 81 25 1 35 115 20 95 3.75e-29 114
MS.gene022302.t1 AT3G61850 65.753 73 23 1 46 118 61 131 3.81e-29 114
MS.gene022302.t1 AT3G61850 65.753 73 23 1 46 118 49 119 5.75e-29 114
MS.gene022302.t1 AT3G61850 65.753 73 23 1 46 118 61 131 5.97e-29 114
MS.gene022302.t1 AT3G52440 68.182 66 21 0 58 123 22 87 1.01e-28 111
MS.gene022302.t1 AT1G28310 82.143 56 10 0 61 116 27 82 1.36e-28 112
MS.gene022302.t1 AT3G52440 68.182 66 21 0 58 123 41 106 1.98e-28 111
MS.gene022302.t1 AT1G28310 82.143 56 10 0 61 116 51 106 2.11e-28 112
MS.gene022302.t1 AT1G21340 69.444 72 19 1 63 134 40 108 2.95e-28 110
MS.gene022302.t1 AT3G21270 54.717 106 42 3 61 164 29 130 4.79e-28 108
MS.gene022302.t1 AT4G21050 59.551 89 33 2 54 142 19 104 5.79e-28 108
MS.gene022302.t1 AT2G28810 65.152 66 22 1 47 112 64 128 5.83e-28 111
MS.gene022302.t1 AT2G28810 65.152 66 22 1 47 112 81 145 1.03e-27 111
MS.gene022302.t1 AT4G00940 53.398 103 31 2 63 151 70 169 8.74e-27 107
MS.gene022302.t1 AT4G00940 53.398 103 31 2 63 151 70 169 8.74e-27 107
MS.gene022302.t1 AT4G00940 53.398 103 31 2 63 151 70 169 8.74e-27 107
MS.gene022302.t1 AT2G46590 55.682 88 36 2 22 108 30 115 2.54e-26 107
MS.gene022302.t1 AT4G38000 60.256 78 30 1 41 117 21 98 2.57e-26 105
MS.gene022302.t1 AT2G46590 55.682 88 36 2 22 108 42 127 2.92e-26 107
MS.gene022302.t1 AT3G50410 75.439 57 14 0 61 117 30 86 7.15e-26 104
MS.gene022302.t1 AT5G66940 70.968 62 18 0 61 122 32 93 1.04e-25 103
MS.gene022302.t1 AT1G47655 73.684 57 13 1 55 109 21 77 5.82e-25 100
MS.gene022302.t1 AT3G47500 63.235 68 24 1 51 118 101 167 4.78e-24 102
MS.gene022302.t1 AT5G62430 46.087 115 48 3 50 164 44 144 5.92e-24 100
MS.gene022302.t1 AT5G39660 63.768 69 24 1 50 118 128 195 9.76e-24 101
MS.gene022302.t1 AT5G39660 63.768 69 24 1 50 118 128 195 9.76e-24 101
MS.gene022302.t1 AT5G39660 63.768 69 24 1 50 118 128 195 9.76e-24 101
MS.gene022302.t1 AT1G69570 64.516 62 21 1 53 114 125 185 3.84e-23 99.4
MS.gene022302.t1 AT1G29160 60.000 70 26 1 45 114 48 115 4.41e-23 94.7
MS.gene022302.t1 AT2G34140 47.253 91 36 1 24 114 33 111 1.44e-22 93.2
MS.gene022302.t1 AT4G21040 57.971 69 29 0 63 131 27 95 4.52e-22 93.6
MS.gene022302.t1 AT1G26790 53.333 75 35 0 58 132 108 182 8.48e-22 95.1
MS.gene022302.t1 AT4G21080 56.000 75 30 1 63 134 27 101 4.18e-21 91.3
MS.gene022302.t1 AT4G21030 55.556 63 25 1 50 112 13 72 6.45e-15 72.8

Find 85 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTGTTCTCCATGAAATT+AGG 0.146876 3.4:+74650127 None:intergenic
AGTAAGTTAGAAGGCATAAT+TGG 0.168612 3.4:-74650052 MS.gene022302:CDS
TCTCACCTAAGTAACTTATT+TGG 0.188613 3.4:-74650172 MS.gene022302:CDS
TAGCAATGGTGCTTATTTAA+TGG 0.193039 3.4:-74649870 MS.gene022302:CDS
AAAGAAAGCTGAAGATCTTT+AGG 0.209545 3.4:+74650211 None:intergenic
CATTCAAGTGACAATAGTTT+AGG 0.231938 3.4:+74649770 None:intergenic
ACTTGTTCTCCATGAAATTA+GGG 0.276978 3.4:+74650128 None:intergenic
GATATGATGAATGGACAAAA+TGG 0.284919 3.4:-74649950 MS.gene022302:CDS
TTTGGAGCTAATGGAGCATT+AGG 0.302981 3.4:-74650154 MS.gene022302:CDS
TGATCATATGAATATGAATA+TGG 0.304551 3.4:-74649990 MS.gene022302:CDS
AGTAACTTATTTGGAGCTAA+TGG 0.308114 3.4:-74650163 MS.gene022302:CDS
GTTCCTAAGTGTTCCTCCTT+TGG 0.313562 3.4:+74650354 None:intergenic
TTCTCTTCACGTCTGCAATA+TGG 0.315298 3.4:-74651092 MS.gene022302:CDS
ATTCAAGTGACAATAGTTTA+GGG 0.332848 3.4:+74649771 None:intergenic
AAGAAAGCTGAAGATCTTTA+GGG 0.336634 3.4:+74650212 None:intergenic
ATGAATATGGGTGTTGGGAT+TGG 0.368536 3.4:-74649977 MS.gene022302:CDS
GCATAATTGGGAGTAGTTCT+AGG 0.373441 3.4:-74650039 MS.gene022302:CDS
CTTTCCATCATGAAGTCAAT+AGG 0.377139 3.4:+74650073 None:intergenic
GTTGTTGTAATAGCAGAATT+TGG 0.387536 3.4:+74650429 None:intergenic
TTTCTACAGCCTCCACCAAC+AGG 0.392353 3.4:+74650328 None:intergenic
AAAGGTCAGTCATTTGGATA+TGG 0.392934 3.4:-74649716 MS.gene022302:CDS
GCAAGACTTGTAGAAGGTAT+TGG 0.393470 3.4:-74650378 MS.gene022302:CDS
GCTGGAAAAGGTCAGTCATT+TGG 0.395618 3.4:-74649722 MS.gene022302:CDS
ATTAGGAAACCCTAATTTCA+TGG 0.397565 3.4:-74650137 MS.gene022302:CDS
AGTGACAATAGTTTAGGGTT+TGG 0.405871 3.4:+74649776 None:intergenic
TTTGGTGGAATGTCATTTGA+TGG 0.425628 3.4:-74649902 MS.gene022302:CDS
TCCTCATCATTAGCATCATA+TGG 0.425855 3.4:+74649824 None:intergenic
GTAAGTTAGAAGGCATAATT+GGG 0.426365 3.4:-74650051 MS.gene022302:CDS
TAGCTCCAAATAAGTTACTT+AGG 0.442973 3.4:+74650167 None:intergenic
AGGTGAGAGAATTGCACATC+AGG 0.445358 3.4:+74650187 None:intergenic
ATCTGCAGATCATTGGTTGC+AGG 0.446436 3.4:-74651065 MS.gene022302:intron
TCTTAGCCTCCTCTCTATCA+TGG 0.458753 3.4:+74650501 None:intergenic
GATCAAGGTTGCTCTGATGC+TGG 0.462649 3.4:-74649740 MS.gene022302:CDS
ATGATGGAAAGTAAGTTAGA+AGG 0.467778 3.4:-74650061 MS.gene022302:CDS
TGGAATCATCTGCAGATCAT+TGG 0.469488 3.4:-74651072 MS.gene022302:CDS
GAGCTTAGCCGTTGATCAAA+GGG 0.477519 3.4:+74649648 None:intergenic
AGTGGTATGATGAATGGTTA+TGG 0.480178 3.4:-74649686 MS.gene022302:CDS
AATATGGGTGTTGGGATTGG+AGG 0.481246 3.4:-74649974 MS.gene022302:CDS
GGTTGCTCTGATGCTGGAAA+AGG 0.482324 3.4:-74649734 MS.gene022302:CDS
GGCACAATTCACGATGAGTC+AGG 0.484101 3.4:-74650580 MS.gene022302:CDS
GATTCATCTTCACCAATGTC+TGG 0.489933 3.4:-74650553 MS.gene022302:CDS
GAGAACAAGTATAGCAATGT+TGG 0.493849 3.4:-74650115 MS.gene022302:CDS
CTCCTCTCTATCATGGATGA+TGG 0.495201 3.4:+74650508 None:intergenic
ACTTCTGCAAGACTTGTAGA+AGG 0.495701 3.4:-74650384 MS.gene022302:CDS
AGAGCTTAGCCGTTGATCAA+AGG 0.500555 3.4:+74649647 None:intergenic
CTAAACTATTGTCACTTGAA+TGG 0.500804 3.4:-74649769 MS.gene022302:CDS
CTTGAATGGCAGCAAGATCA+AGG 0.502791 3.4:-74649755 MS.gene022302:CDS
ATGAATATGAATATGGGTGT+TGG 0.503188 3.4:-74649983 MS.gene022302:CDS
AGTCATTTGGATATGGATCA+TGG 0.505387 3.4:-74649709 MS.gene022302:CDS
GTGACATGCTAACATGTTCA+AGG 0.508689 3.4:-74650531 MS.gene022302:CDS
TGAATATGAATATGGGTGTT+GGG 0.514370 3.4:-74649982 MS.gene022302:CDS
ATCATGAAGTCAATAGGCCT+AGG 0.516098 3.4:+74650079 None:intergenic
CTTAGGAACATCCCTGTTGG+TGG 0.550577 3.4:-74650340 MS.gene022302:CDS
CAAGTCTTGCAGAAGTACCT+TGG 0.558008 3.4:+74650391 None:intergenic
AGGAACATCCCTGTTGGTGG+AGG 0.558859 3.4:-74650337 MS.gene022302:CDS
ACAACAAACCCTTTGATCAA+CGG 0.565654 3.4:-74649656 MS.gene022302:CDS
ATATGATGAATGGACAAAAT+GGG 0.566744 3.4:-74649949 MS.gene022302:CDS
ATTGGAGGAGATATGATGAA+TGG 0.570060 3.4:-74649959 MS.gene022302:CDS
ACCTATCCCTAAAATGTCCA+AGG 0.579764 3.4:-74650468 MS.gene022302:CDS
GATCATATGAATATGAATAT+GGG 0.580380 3.4:-74649989 MS.gene022302:CDS
TAGGCCTATTGACTTCATGA+TGG 0.581168 3.4:-74650077 MS.gene022302:CDS
TCATGGAGTGGTATGATGAA+TGG 0.582721 3.4:-74649692 MS.gene022302:CDS
TGTAGAAGGTATTGGACCAA+AGG 0.594254 3.4:-74650370 MS.gene022302:CDS
AATTCACGATGAGTCAGGAA+TGG 0.596611 3.4:-74650575 MS.gene022302:CDS
GGACCAAAGGAGGAACACTT+AGG 0.599656 3.4:-74650357 MS.gene022302:CDS
AATGCATGATGAAAATTCTG+AGG 0.599697 3.4:+74649923 None:intergenic
AGGAAGGTACATGATGTGGT+TGG 0.607867 3.4:+74650245 None:intergenic
GGTGGAATGTCATTTGATGG+AGG 0.611132 3.4:-74649899 MS.gene022302:CDS
TCATGAAGTCAATAGGCCTA+GGG 0.612063 3.4:+74650080 None:intergenic
GGCCATCATCCATGATAGAG+AGG 0.614387 3.4:-74650510 MS.gene022302:CDS
GATGGAGGAAACAATAGCAA+TGG 0.614470 3.4:-74649884 MS.gene022302:CDS
GGTGAGAGAATTGCACATCA+GGG 0.615522 3.4:+74650188 None:intergenic
TTGGCATGCTTGAAAACCCT+AGG 0.618350 3.4:-74650096 MS.gene022302:CDS
AGAAGGTATTGGACCAAAGG+AGG 0.622045 3.4:-74650367 MS.gene022302:CDS
GTTAGCATGTCACCAGACAT+TGG 0.624948 3.4:+74650541 None:intergenic
TTCTACAGCCTCCACCAACA+GGG 0.626747 3.4:+74650329 None:intergenic
TGGTAGGAAGGTACATGATG+TGG 0.632743 3.4:+74650241 None:intergenic
ACACTTAGGAACATCCCTGT+TGG 0.646210 3.4:-74650343 MS.gene022302:CDS
TTTGGATATGGATCATGGAG+TGG 0.646639 3.4:-74649704 MS.gene022302:CDS
GTTGATCAAAGGGTTTGTTG+TGG 0.647747 3.4:+74649658 None:intergenic
TTTAGGGATAGGTCATGTGG+TGG 0.663306 3.4:+74650478 None:intergenic
ACTACAGTCTCTCTCAGCCA+AGG 0.663924 3.4:-74650408 MS.gene022302:CDS
GTGTGAGTTGAATCACACCT+TGG 0.665322 3.4:+74650451 None:intergenic
TCCATATGATGCTAATGATG+AGG 0.665364 3.4:-74649825 MS.gene022302:CDS
CATCATCCATGATAGAGAGG+AGG 0.679330 3.4:-74650507 MS.gene022302:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGGAATTTTGATAATTATTT+TGG - chr3.4:74650736-74650755 MS.gene022302:intron 15.0%
!! AACATGATAAACAAAATGAA+AGG + chr3.4:74649747-74649766 None:intergenic 20.0%
!! AAGCTAAGAAAAACAAAATT+AGG - chr3.4:74650001-74650020 MS.gene022302:CDS 20.0%
!! ACATGCATTTATCAAATTAA+TGG - chr3.4:74649781-74649800 MS.gene022302:CDS 20.0%
!! AGCTAAGAAAAACAAAATTA+GGG - chr3.4:74650002-74650021 MS.gene022302:CDS 20.0%
!! GATCATATGAATATGAATAT+GGG - chr3.4:74650766-74650785 MS.gene022302:intron 20.0%
!! TAAACAAAATGAAAGGAAAA+TGG + chr3.4:74649740-74649759 None:intergenic 20.0%
!! TGATCATATGAATATGAATA+TGG - chr3.4:74650765-74650784 MS.gene022302:intron 20.0%
!!! TAAGTGATCATTTGATTTTT+TGG + chr3.4:74650470-74650489 None:intergenic 20.0%
!!! TCTTTTATACAAAATTGTCA+GGG - chr3.4:74650154-74650173 MS.gene022302:CDS 20.0%
!!! TTCTTTTATACAAAATTGTC+AGG - chr3.4:74650153-74650172 MS.gene022302:CDS 20.0%
! AAATTAATGGTGTAGTAGTT+TGG - chr3.4:74649794-74649813 MS.gene022302:CDS 25.0%
! ATATGATGAATGGACAAAAT+GGG - chr3.4:74650806-74650825 MS.gene022302:intron 25.0%
! ATTCAAGTGACAATAGTTTA+GGG + chr3.4:74650987-74651006 None:intergenic 25.0%
!! GAAAAGAGAATTTTATGCTT+AGG - chr3.4:74649816-74649835 MS.gene022302:CDS 25.0%
!!! AATGTGTTAATTTGTCTTGT+TGG + chr3.4:74650093-74650112 None:intergenic 25.0%
!!! TGGAATTGGATTATTTTACA+TGG + chr3.4:74650073-74650092 None:intergenic 25.0%
!!! TTAATTTGTCTTGTTGGAAT+TGG + chr3.4:74650087-74650106 None:intergenic 25.0%
!!! TTCACTTTTAATCATGAGAT+CGG + chr3.4:74649956-74649975 None:intergenic 25.0%
AAAATGGTATACATTGTGCA+TGG + chr3.4:74649724-74649743 None:intergenic 30.0%
AAAGAAAGCTGAAGATCTTT+AGG + chr3.4:74650547-74650566 None:intergenic 30.0%
AAGAAAGCTGAAGATCTTTA+GGG + chr3.4:74650546-74650565 None:intergenic 30.0%
AATGCATGATGAAAATTCTG+AGG + chr3.4:74650835-74650854 None:intergenic 30.0%
ACTTGTTCTCCATGAAATTA+GGG + chr3.4:74650630-74650649 None:intergenic 30.0%
ATGAATATGAATATGGGTGT+TGG - chr3.4:74650772-74650791 MS.gene022302:intron 30.0%
ATTAGGAAACCCTAATTTCA+TGG - chr3.4:74650618-74650637 MS.gene022302:intron 30.0%
CATGATCATTTGAATTGCTA+TGG - chr3.4:74649891-74649910 MS.gene022302:CDS 30.0%
CATTCAAGTGACAATAGTTT+AGG + chr3.4:74650988-74651007 None:intergenic 30.0%
GATATGATGAATGGACAAAA+TGG - chr3.4:74650805-74650824 MS.gene022302:intron 30.0%
GATCTCATGATTAAAAGTGA+AGG - chr3.4:74649955-74649974 MS.gene022302:CDS 30.0%
GTTGTTGTAATAGCAGAATT+TGG + chr3.4:74650329-74650348 None:intergenic 30.0%
TACTTGTTCTCCATGAAATT+AGG + chr3.4:74650631-74650650 None:intergenic 30.0%
TAGCAATGGTGCTTATTTAA+TGG - chr3.4:74650885-74650904 MS.gene022302:intron 30.0%
TAGCTCCAAATAAGTTACTT+AGG + chr3.4:74650591-74650610 None:intergenic 30.0%
TCTCACCTAAGTAACTTATT+TGG - chr3.4:74650583-74650602 MS.gene022302:CDS 30.0%
TGAATATGAATATGGGTGTT+GGG - chr3.4:74650773-74650792 MS.gene022302:intron 30.0%
! AATTTTCATCATGCATTTGG+TGG - chr3.4:74650838-74650857 MS.gene022302:intron 30.0%
! AGTAACTTATTTGGAGCTAA+TGG - chr3.4:74650592-74650611 MS.gene022302:intron 30.0%
! AGTAAGTTAGAAGGCATAAT+TGG - chr3.4:74650703-74650722 MS.gene022302:intron 30.0%
! ATGATGGAAAGTAAGTTAGA+AGG - chr3.4:74650694-74650713 MS.gene022302:intron 30.0%
! CAGAATTTTCATCATGCATT+TGG - chr3.4:74650835-74650854 MS.gene022302:intron 30.0%
! CTAAACTATTGTCACTTGAA+TGG - chr3.4:74650986-74651005 MS.gene022302:intron 30.0%
! GTAAGTTAGAAGGCATAATT+GGG - chr3.4:74650704-74650723 MS.gene022302:intron 30.0%
AAAGGTCAGTCATTTGGATA+TGG - chr3.4:74651039-74651058 MS.gene022302:intron 35.0%
ACAACAAACCCTTTGATCAA+CGG - chr3.4:74651099-74651118 MS.gene022302:CDS 35.0%
AGTCATTTGGATATGGATCA+TGG - chr3.4:74651046-74651065 MS.gene022302:intron 35.0%
AGTGGTATGATGAATGGTTA+TGG - chr3.4:74651069-74651088 MS.gene022302:CDS 35.0%
ATTGGAGGAGATATGATGAA+TGG - chr3.4:74650796-74650815 MS.gene022302:intron 35.0%
CTTTCCATCATGAAGTCAAT+AGG + chr3.4:74650685-74650704 None:intergenic 35.0%
GAGAACAAGTATAGCAATGT+TGG - chr3.4:74650640-74650659 MS.gene022302:intron 35.0%
TCCATATGATGCTAATGATG+AGG - chr3.4:74650930-74650949 MS.gene022302:intron 35.0%
TCCTCATCATTAGCATCATA+TGG + chr3.4:74650934-74650953 None:intergenic 35.0%
! AATCACACCTTGGACATTTT+AGG + chr3.4:74650297-74650316 None:intergenic 35.0%
! AGTGACAATAGTTTAGGGTT+TGG + chr3.4:74650982-74651001 None:intergenic 35.0%
! ATCACACCTTGGACATTTTA+GGG + chr3.4:74650296-74650315 None:intergenic 35.0%
! TTTGGTGGAATGTCATTTGA+TGG - chr3.4:74650853-74650872 MS.gene022302:intron 35.0%
!!! ATCTTTAGGGTTTTGATGGT+AGG + chr3.4:74650533-74650552 None:intergenic 35.0%
!!! GAAGATCTTTAGGGTTTTGA+TGG + chr3.4:74650537-74650556 None:intergenic 35.0%
AATTCACGATGAGTCAGGAA+TGG - chr3.4:74650180-74650199 MS.gene022302:CDS 40.0%
ACCTATCCCTAAAATGTCCA+AGG - chr3.4:74650287-74650306 MS.gene022302:CDS 40.0%
ACTTCTGCAAGACTTGTAGA+AGG - chr3.4:74650371-74650390 MS.gene022302:CDS 40.0%
ATCATGAAGTCAATAGGCCT+AGG + chr3.4:74650679-74650698 None:intergenic 40.0%
GATGGAGGAAACAATAGCAA+TGG - chr3.4:74650871-74650890 MS.gene022302:intron 40.0%
GATTCATCTTCACCAATGTC+TGG - chr3.4:74650202-74650221 MS.gene022302:CDS 40.0%
GCATAATTGGGAGTAGTTCT+AGG - chr3.4:74650716-74650735 MS.gene022302:intron 40.0%
GTGACATGCTAACATGTTCA+AGG - chr3.4:74650224-74650243 MS.gene022302:CDS 40.0%
TCATGAAGTCAATAGGCCTA+GGG + chr3.4:74650678-74650697 None:intergenic 40.0%
TCATGGAGTGGTATGATGAA+TGG - chr3.4:74651063-74651082 MS.gene022302:intron 40.0%
TGGAATCATCTGCAGATCAT+TGG - chr3.4:74649683-74649702 MS.gene022302:CDS 40.0%
TTCTCTTCACGTCTGCAATA+TGG - chr3.4:74649663-74649682 MS.gene022302:CDS 40.0%
TTTGGATATGGATCATGGAG+TGG - chr3.4:74651051-74651070 MS.gene022302:intron 40.0%
! GCAAGACTTGTAGAAGGTAT+TGG - chr3.4:74650377-74650396 MS.gene022302:CDS 40.0%
! TTTGGAGCTAATGGAGCATT+AGG - chr3.4:74650601-74650620 MS.gene022302:intron 40.0%
!! ACCTTGGACATTTTAGGGAT+AGG + chr3.4:74650291-74650310 None:intergenic 40.0%
!! ATGAATATGGGTGTTGGGAT+TGG - chr3.4:74650778-74650797 MS.gene022302:intron 40.0%
!! CATTTTAGGGATAGGTCATG+TGG + chr3.4:74650283-74650302 None:intergenic 40.0%
!! GTTGATCAAAGGGTTTGTTG+TGG + chr3.4:74651100-74651119 None:intergenic 40.0%
!! TAGGCCTATTGACTTCATGA+TGG - chr3.4:74650678-74650697 MS.gene022302:intron 40.0%
!! TGTAGAAGGTATTGGACCAA+AGG - chr3.4:74650385-74650404 MS.gene022302:CDS 40.0%
!! TTAGGGTTTTGATGGTAGGA+AGG + chr3.4:74650529-74650548 None:intergenic 40.0%
ACACTTAGGAACATCCCTGT+TGG - chr3.4:74650412-74650431 MS.gene022302:CDS 45.0%
AGGTGAGAGAATTGCACATC+AGG + chr3.4:74650571-74650590 None:intergenic 45.0%
CATCATCCATGATAGAGAGG+AGG - chr3.4:74650248-74650267 MS.gene022302:CDS 45.0%
CTCCTCTCTATCATGGATGA+TGG + chr3.4:74650250-74650269 None:intergenic 45.0%
CTTGAATGGCAGCAAGATCA+AGG - chr3.4:74651000-74651019 MS.gene022302:intron 45.0%
GCTGGAAAAGGTCAGTCATT+TGG - chr3.4:74651033-74651052 MS.gene022302:intron 45.0%
GGTGAGAGAATTGCACATCA+GGG + chr3.4:74650570-74650589 None:intergenic 45.0%
GTTAGCATGTCACCAGACAT+TGG + chr3.4:74650217-74650236 None:intergenic 45.0%
GTTCCTAAGTGTTCCTCCTT+TGG + chr3.4:74650404-74650423 None:intergenic 45.0%
TCTTAGCCTCCTCTCTATCA+TGG + chr3.4:74650257-74650276 None:intergenic 45.0%
TTGGCATGCTTGAAAACCCT+AGG - chr3.4:74650659-74650678 MS.gene022302:intron 45.0%
TTTAGGGATAGGTCATGTGG+TGG + chr3.4:74650280-74650299 None:intergenic 45.0%
! AGGAAGGTACATGATGTGGT+TGG + chr3.4:74650513-74650532 None:intergenic 45.0%
! ATCTGCAGATCATTGGTTGC+AGG - chr3.4:74649690-74649709 MS.gene022302:CDS 45.0%
! GGTGGAATGTCATTTGATGG+AGG - chr3.4:74650856-74650875 MS.gene022302:intron 45.0%
! GTGTGAGTTGAATCACACCT+TGG + chr3.4:74650307-74650326 None:intergenic 45.0%
! TGGTAGGAAGGTACATGATG+TGG + chr3.4:74650517-74650536 None:intergenic 45.0%
!! AATATGGGTGTTGGGATTGG+AGG - chr3.4:74650781-74650800 MS.gene022302:intron 45.0%
!! AGAAGGTATTGGACCAAAGG+AGG - chr3.4:74650388-74650407 MS.gene022302:CDS 45.0%
!! CAAGTCTTGCAGAAGTACCT+TGG + chr3.4:74650367-74650386 None:intergenic 45.0%
ACTACAGTCTCTCTCAGCCA+AGG - chr3.4:74650347-74650366 MS.gene022302:CDS 50.0%
CTTAGGAACATCCCTGTTGG+TGG - chr3.4:74650415-74650434 MS.gene022302:CDS 50.0%
GATCAAGGTTGCTCTGATGC+TGG - chr3.4:74651015-74651034 MS.gene022302:intron 50.0%
GGACCAAAGGAGGAACACTT+AGG - chr3.4:74650398-74650417 MS.gene022302:CDS 50.0%
GGCACAATTCACGATGAGTC+AGG - chr3.4:74650175-74650194 MS.gene022302:CDS 50.0%
GGCCATCATCCATGATAGAG+AGG - chr3.4:74650245-74650264 MS.gene022302:CDS 50.0%
GGTTGCTCTGATGCTGGAAA+AGG - chr3.4:74651021-74651040 MS.gene022302:intron 50.0%
TTCTACAGCCTCCACCAACA+GGG + chr3.4:74650429-74650448 None:intergenic 50.0%
TTTCTACAGCCTCCACCAAC+AGG + chr3.4:74650430-74650449 None:intergenic 50.0%
! AGGAACATCCCTGTTGGTGG+AGG - chr3.4:74650418-74650437 MS.gene022302:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 74649652 74651125 74649652 ID=MS.gene022302
chr3.4 mRNA 74649652 74651125 74649652 ID=MS.gene022302.t1;Parent=MS.gene022302
chr3.4 exon 74651066 74651125 74651066 ID=MS.gene022302.t1.exon1;Parent=MS.gene022302.t1
chr3.4 CDS 74651066 74651125 74651066 ID=cds.MS.gene022302.t1;Parent=MS.gene022302.t1
chr3.4 exon 74649652 74650602 74649652 ID=MS.gene022302.t1.exon2;Parent=MS.gene022302.t1
chr3.4 CDS 74649652 74650602 74649652 ID=cds.MS.gene022302.t1;Parent=MS.gene022302.t1
Gene Sequence

>MS.gene022302

ATGGGTTTATCTTCTCTTCACGTCTGCAATATGGAATCATCTGCAGATCATTGGTTGCAGGTATATATACCATGCACAATGTATACCATTTTCCTTTCATTTTGTTTATCATGTTCACATATCACTAACACATGCATTTATCAAATTAATGGTGTAGTAGTTTGGAAAAGAGAATTTTATGCTTAGGTTTATGCTTTGTAAATAACTATGTTTTGTACATTAATTAGCTTAATTAGATTCATGATCATTTGAATTGCTATGGAATTTAATTAAAAGTGCTTTGTATAGTATATGTTGAACTCCGATCTCATGATTAAAAGTGAAGGAGAACGAAAAATAAAAGAAGAGAAAGCTAAGAAAAACAAAATTAGGGAGAAGCTAGCTAGAAAGAGAACAAAAAGAATGAAAAAGAAAAAGACCATGTAAAATAATCCAATTCCAACAAGACAAATTAACACATTTTTCACTTTTACTTTTATCATAAATATATTAACTTCTAGTTTCTTTTATACAAAATTGTCAGGGCACAATTCACGATGAGTCAGGAATGGATTCATCTTCACCAATGTCTGGTGACATGCTAACATGTTCAAGGCCATCATCCATGATAGAGAGGAGGCTAAGACCACCACATGACCTATCCCTAAAATGTCCAAGGTGTGATTCAACTCACACCAAATTCTGCTATTACAACAACTACAGTCTCTCTCAGCCAAGGTACTTCTGCAAGACTTGTAGAAGGTATTGGACCAAAGGAGGAACACTTAGGAACATCCCTGTTGGTGGAGGCTGTAGAAAAAACAAAAAAGTTTCTTCCAAAAAATCAAATGATCACTTAGCTAATAATAATATTCAAAACCAACCACATCATGTACCTTCCTACCATCAAAACCCTAAAGATCTTCAGCTTTCTTTCCCTGATGTGCAATTCTCTCACCTAAGTAACTTATTTGGAGCTAATGGAGCATTAGGAAACCCTAATTTCATGGAGAACAAGTATAGCAATGTTGGCATGCTTGAAAACCCTAGGCCTATTGACTTCATGATGGAAAGTAAGTTAGAAGGCATAATTGGGAGTAGTTCTAGGAATTTTGATAATTATTTTGGAAATAGTGATCATATGAATATGAATATGGGTGTTGGGATTGGAGGAGATATGATGAATGGACAAAATGGGTTGCCTCAGAATTTTCATCATGCATTTGGTGGAATGTCATTTGATGGAGGAAACAATAGCAATGGTGCTTATTTAATGGATTCTTGTCAAAGACTTATGCTTCCATATGATGCTAATGATGAGGATCATAATGCTTCAATTGATGTGAAGCCAAACCCTAAACTATTGTCACTTGAATGGCAGCAAGATCAAGGTTGCTCTGATGCTGGAAAAGGTCAGTCATTTGGATATGGATCATGGAGTGGTATGATGAATGGTTATGGATCTTCCACAACAAACCCTTTGATCAACGGCTAA
Protein sequence

>MS.gene022302.t1

MGLSSLHVCNMESSADHWLQGTIHDESGMDSSSPMSGDMLTCSRPSSMIERRLRPPHDLSLKCPRCDSTHTKFCYYNNYSLSQPRYFCKTCRRYWTKGGTLRNIPVGGGCRKNKKVSSKKSNDHLANNNIQNQPHHVPSYHQNPKDLQLSFPDVQFSHLSNLFGANGALGNPNFMENKYSNVGMLENPRPIDFMMESKLEGIIGSSSRNFDNYFGNSDHMNMNMGVGIGGDMMNGQNGLPQNFHHAFGGMSFDGGNNSNGAYLMDSCQRLMLPYDANDEDHNASIDVKPNPKLLSLEWQQDQGCSDAGKGQSFGYGSWSGMMNGYGSSTTNPLING