Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022331.t1 | XP_003601210.2 | 100 | 152 | 0 | 0 | 1 | 152 | 1 | 152 | 1.50E-81 | 312.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022331.t1 | Q9FML4 | 74.0 | 146 | 21 | 1 | 2 | 130 | 3 | 148 | 4.1e-56 | 219.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022331.t1 | H2D439 | 100.0 | 152 | 0 | 0 | 1 | 152 | 1 | 152 | 1.1e-81 | 312.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene022331.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022331.t1 | MTR_3g077240 | 100.000 | 191 | 0 | 0 | 1 | 191 | 1 | 191 | 1.21e-141 | 392 |
| MS.gene022331.t1 | MTR_4g105170 | 75.000 | 176 | 34 | 4 | 7 | 177 | 8 | 178 | 4.47e-86 | 252 |
| MS.gene022331.t1 | MTR_7g033800 | 59.278 | 194 | 56 | 3 | 1 | 193 | 1 | 172 | 2.13e-71 | 214 |
| MS.gene022331.t1 | MTR_6g005070 | 64.151 | 159 | 30 | 2 | 1 | 159 | 1 | 132 | 1.29e-65 | 198 |
| MS.gene022331.t1 | MTR_6g005080 | 78.261 | 115 | 25 | 0 | 1 | 115 | 1 | 115 | 4.91e-64 | 194 |
| MS.gene022331.t1 | MTR_8g040900 | 75.221 | 113 | 25 | 1 | 1 | 113 | 1 | 110 | 3.85e-52 | 167 |
| MS.gene022331.t1 | MTR_5g017950 | 55.556 | 153 | 58 | 1 | 4 | 156 | 2 | 144 | 2.29e-51 | 167 |
| MS.gene022331.t1 | MTR_5g083230 | 71.154 | 104 | 30 | 0 | 8 | 111 | 4 | 107 | 3.47e-50 | 160 |
| MS.gene022331.t1 | MTR_2g068760 | 56.204 | 137 | 60 | 0 | 9 | 145 | 12 | 148 | 3.78e-49 | 157 |
| MS.gene022331.t1 | MTR_3g073690 | 54.140 | 157 | 68 | 2 | 4 | 157 | 3 | 158 | 3.67e-48 | 156 |
| MS.gene022331.t1 | MTR_4g083680 | 65.714 | 105 | 36 | 0 | 9 | 113 | 14 | 118 | 2.77e-47 | 153 |
| MS.gene022331.t1 | MTR_3g452660 | 55.245 | 143 | 64 | 0 | 5 | 147 | 2 | 144 | 8.13e-47 | 155 |
| MS.gene022331.t1 | MTR_5g083010 | 53.571 | 140 | 54 | 1 | 8 | 136 | 9 | 148 | 9.09e-47 | 152 |
| MS.gene022331.t1 | MTR_3g031660 | 64.423 | 104 | 37 | 0 | 8 | 111 | 5 | 108 | 2.61e-46 | 150 |
| MS.gene022331.t1 | MTR_3g071590 | 66.346 | 104 | 35 | 0 | 8 | 111 | 6 | 109 | 8.35e-46 | 149 |
| MS.gene022331.t1 | MTR_5g083960 | 51.408 | 142 | 60 | 2 | 8 | 140 | 37 | 178 | 8.14e-44 | 145 |
| MS.gene022331.t1 | MTR_3g094690 | 60.550 | 109 | 43 | 0 | 9 | 117 | 46 | 154 | 1.99e-43 | 145 |
| MS.gene022331.t1 | MTR_3g071420 | 65.625 | 96 | 33 | 0 | 8 | 103 | 12 | 107 | 2.29e-42 | 140 |
| MS.gene022331.t1 | MTR_2g093310 | 55.963 | 109 | 48 | 0 | 9 | 117 | 32 | 140 | 2.30e-42 | 141 |
| MS.gene022331.t1 | MTR_3g094690 | 59.633 | 109 | 43 | 1 | 9 | 117 | 46 | 153 | 6.76e-41 | 138 |
| MS.gene022331.t1 | MTR_4g060950 | 55.455 | 110 | 48 | 1 | 9 | 117 | 23 | 132 | 1.07e-39 | 134 |
| MS.gene022331.t1 | MTR_1g095850 | 58.252 | 103 | 43 | 0 | 11 | 113 | 73 | 175 | 4.29e-39 | 134 |
| MS.gene022331.t1 | MTR_5g075020 | 57.692 | 130 | 45 | 2 | 9 | 128 | 42 | 171 | 4.85e-39 | 134 |
| MS.gene022331.t1 | MTR_5g075020 | 57.692 | 130 | 45 | 2 | 9 | 128 | 46 | 175 | 9.55e-39 | 133 |
| MS.gene022331.t1 | MTR_7g096530 | 57.798 | 109 | 45 | 1 | 3 | 110 | 7 | 115 | 2.89e-38 | 131 |
| MS.gene022331.t1 | MTR_8g036085 | 57.143 | 105 | 44 | 1 | 10 | 113 | 23 | 127 | 2.55e-37 | 129 |
| MS.gene022331.t1 | MTR_8g036105 | 56.410 | 117 | 50 | 1 | 10 | 125 | 18 | 134 | 1.04e-36 | 127 |
| MS.gene022331.t1 | MTR_1g070220 | 54.701 | 117 | 47 | 2 | 1 | 111 | 1 | 117 | 8.20e-36 | 124 |
| MS.gene022331.t1 | MTR_7g074990 | 61.053 | 95 | 36 | 1 | 10 | 103 | 20 | 114 | 9.42e-35 | 122 |
| MS.gene022331.t1 | MTR_7g028905 | 50.909 | 110 | 51 | 1 | 9 | 118 | 4 | 110 | 1.89e-34 | 118 |
| MS.gene022331.t1 | MTR_6g027710 | 51.818 | 110 | 50 | 2 | 9 | 115 | 39 | 148 | 2.13e-33 | 119 |
| MS.gene022331.t1 | MTR_8g079660 | 46.789 | 109 | 58 | 0 | 10 | 118 | 8 | 116 | 3.74e-32 | 117 |
| MS.gene022331.t1 | MTR_5g080470 | 51.485 | 101 | 48 | 1 | 5 | 104 | 2 | 102 | 3.78e-32 | 115 |
| MS.gene022331.t1 | MTR_7g096610 | 57.292 | 96 | 40 | 1 | 9 | 103 | 7 | 102 | 5.93e-32 | 115 |
| MS.gene022331.t1 | MTR_1g070205 | 54.737 | 95 | 42 | 1 | 10 | 103 | 12 | 106 | 1.53e-31 | 114 |
| MS.gene022331.t1 | MTR_7g075230 | 56.731 | 104 | 42 | 2 | 10 | 110 | 12 | 115 | 3.05e-31 | 112 |
| MS.gene022331.t1 | MTR_5g093010 | 44.628 | 121 | 65 | 1 | 11 | 129 | 6 | 126 | 1.82e-30 | 109 |
| MS.gene022331.t1 | MTR_8g079620 | 47.115 | 104 | 55 | 0 | 10 | 113 | 8 | 111 | 7.71e-30 | 110 |
| MS.gene022331.t1 | MTR_4g107450 | 48.000 | 100 | 52 | 0 | 11 | 110 | 11 | 110 | 4.39e-29 | 109 |
| MS.gene022331.t1 | MTR_8g079580 | 46.364 | 110 | 58 | 1 | 10 | 118 | 8 | 117 | 5.17e-28 | 105 |
| MS.gene022331.t1 | MTR_6g011200 | 45.918 | 98 | 52 | 1 | 8 | 104 | 7 | 104 | 9.14e-28 | 105 |
| MS.gene022331.t1 | MTR_6g027700 | 47.000 | 100 | 52 | 1 | 10 | 108 | 7 | 106 | 2.86e-27 | 102 |
| MS.gene022331.t1 | MTR_8g017090 | 43.689 | 103 | 58 | 0 | 11 | 113 | 13 | 115 | 4.04e-26 | 102 |
| MS.gene022331.t1 | MTR_6g011230 | 35.252 | 139 | 85 | 2 | 11 | 145 | 12 | 149 | 3.97e-25 | 97.1 |
| MS.gene022331.t1 | MTR_6g011250 | 39.370 | 127 | 72 | 2 | 8 | 129 | 7 | 133 | 6.29e-24 | 93.6 |
| MS.gene022331.t1 | MTR_4g099230 | 41.053 | 95 | 55 | 1 | 11 | 104 | 3 | 97 | 3.17e-23 | 90.9 |
| MS.gene022331.t1 | MTR_2g100020 | 43.011 | 93 | 53 | 0 | 11 | 103 | 20 | 112 | 3.36e-23 | 93.6 |
| MS.gene022331.t1 | MTR_5g085390 | 39.423 | 104 | 62 | 1 | 8 | 110 | 7 | 110 | 7.70e-20 | 84.0 |
| MS.gene022331.t1 | MTR_4g088035 | 29.878 | 164 | 98 | 4 | 9 | 163 | 5 | 160 | 3.08e-18 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022331.t1 | AT5G63090 | 75.000 | 148 | 19 | 2 | 1 | 130 | 1 | 148 | 3.42e-73 | 219 |
| MS.gene022331.t1 | AT5G63090 | 75.000 | 148 | 19 | 2 | 1 | 130 | 1 | 148 | 3.42e-73 | 219 |
| MS.gene022331.t1 | AT5G63090 | 75.000 | 148 | 19 | 2 | 1 | 130 | 1 | 148 | 3.42e-73 | 219 |
| MS.gene022331.t1 | AT5G63090 | 75.000 | 148 | 19 | 2 | 1 | 130 | 1 | 148 | 3.42e-73 | 219 |
| MS.gene022331.t1 | AT3G27650 | 79.279 | 111 | 23 | 0 | 4 | 114 | 33 | 143 | 4.04e-63 | 192 |
| MS.gene022331.t1 | AT1G65620 | 73.451 | 113 | 29 | 1 | 1 | 113 | 1 | 112 | 3.81e-51 | 164 |
| MS.gene022331.t1 | AT1G65620 | 73.451 | 113 | 29 | 1 | 1 | 113 | 1 | 112 | 3.81e-51 | 164 |
| MS.gene022331.t1 | AT1G65620 | 73.451 | 113 | 29 | 1 | 1 | 113 | 1 | 112 | 3.81e-51 | 164 |
| MS.gene022331.t1 | AT1G65620 | 73.451 | 113 | 29 | 1 | 1 | 113 | 1 | 112 | 3.81e-51 | 164 |
| MS.gene022331.t1 | AT1G65620 | 73.451 | 113 | 29 | 1 | 1 | 113 | 1 | 112 | 3.81e-51 | 164 |
| MS.gene022331.t1 | AT5G66870 | 62.791 | 129 | 41 | 2 | 1 | 129 | 1 | 122 | 2.09e-50 | 165 |
| MS.gene022331.t1 | AT2G23660 | 60.504 | 119 | 43 | 1 | 8 | 126 | 3 | 117 | 7.10e-49 | 161 |
| MS.gene022331.t1 | AT2G23660 | 60.504 | 119 | 43 | 1 | 8 | 126 | 3 | 117 | 7.10e-49 | 161 |
| MS.gene022331.t1 | AT2G23660 | 60.504 | 119 | 43 | 1 | 8 | 126 | 3 | 117 | 7.10e-49 | 161 |
| MS.gene022331.t1 | AT2G30130 | 69.231 | 104 | 32 | 0 | 8 | 111 | 6 | 109 | 8.15e-49 | 157 |
| MS.gene022331.t1 | AT2G30340 | 57.759 | 116 | 49 | 0 | 9 | 124 | 52 | 167 | 4.32e-44 | 147 |
| MS.gene022331.t1 | AT2G30340 | 57.759 | 116 | 49 | 0 | 9 | 124 | 51 | 166 | 4.35e-44 | 147 |
| MS.gene022331.t1 | AT1G31320 | 72.727 | 88 | 24 | 0 | 9 | 96 | 12 | 99 | 1.37e-43 | 143 |
| MS.gene022331.t1 | AT1G07900 | 58.333 | 108 | 45 | 0 | 9 | 116 | 32 | 139 | 2.34e-43 | 143 |
| MS.gene022331.t1 | AT1G16530 | 62.264 | 106 | 39 | 1 | 9 | 113 | 13 | 118 | 1.31e-42 | 140 |
| MS.gene022331.t1 | AT2G28500 | 60.194 | 103 | 41 | 0 | 9 | 111 | 54 | 156 | 2.38e-41 | 139 |
| MS.gene022331.t1 | AT3G11090 | 54.167 | 120 | 52 | 2 | 8 | 127 | 9 | 125 | 1.53e-39 | 133 |
| MS.gene022331.t1 | AT3G26660 | 56.000 | 100 | 44 | 0 | 11 | 110 | 6 | 105 | 2.73e-38 | 128 |
| MS.gene022331.t1 | AT3G26620 | 56.000 | 100 | 44 | 0 | 11 | 110 | 6 | 105 | 2.95e-38 | 128 |
| MS.gene022331.t1 | AT2G40470 | 53.676 | 136 | 50 | 2 | 9 | 139 | 19 | 146 | 3.59e-38 | 130 |
| MS.gene022331.t1 | AT2G40470 | 53.676 | 136 | 50 | 2 | 9 | 139 | 44 | 171 | 7.23e-38 | 130 |
| MS.gene022331.t1 | AT3G03760 | 55.769 | 104 | 45 | 1 | 9 | 111 | 50 | 153 | 3.49e-36 | 127 |
| MS.gene022331.t1 | AT2G45420 | 59.596 | 99 | 39 | 1 | 13 | 110 | 40 | 138 | 2.86e-35 | 125 |
| MS.gene022331.t1 | AT4G00220 | 59.596 | 99 | 39 | 1 | 13 | 110 | 20 | 118 | 3.67e-35 | 124 |
| MS.gene022331.t1 | AT5G06080 | 42.405 | 158 | 87 | 3 | 5 | 161 | 2 | 156 | 5.93e-34 | 119 |
| MS.gene022331.t1 | AT2G42430 | 55.340 | 103 | 45 | 1 | 9 | 110 | 14 | 116 | 1.06e-33 | 120 |
| MS.gene022331.t1 | AT2G45410 | 55.085 | 118 | 47 | 3 | 13 | 126 | 19 | 134 | 2.58e-33 | 117 |
| MS.gene022331.t1 | AT4G00210 | 49.600 | 125 | 54 | 2 | 3 | 126 | 4 | 120 | 2.59e-33 | 119 |
| MS.gene022331.t1 | AT2G31310 | 55.208 | 96 | 42 | 1 | 9 | 103 | 6 | 101 | 1.15e-32 | 116 |
| MS.gene022331.t1 | AT3G58190 | 55.208 | 96 | 42 | 1 | 10 | 104 | 11 | 106 | 1.93e-32 | 116 |
| MS.gene022331.t1 | AT2G42440 | 52.577 | 97 | 45 | 1 | 9 | 104 | 6 | 102 | 8.02e-32 | 115 |
| MS.gene022331.t1 | AT5G35900 | 46.610 | 118 | 63 | 0 | 11 | 128 | 6 | 123 | 3.18e-31 | 113 |
| MS.gene022331.t1 | AT3G50510 | 44.915 | 118 | 62 | 1 | 9 | 126 | 11 | 125 | 4.55e-30 | 109 |
| MS.gene022331.t1 | AT3G50510 | 44.915 | 118 | 62 | 1 | 9 | 126 | 11 | 125 | 4.55e-30 | 109 |
| MS.gene022331.t1 | AT3G13850 | 44.660 | 103 | 57 | 0 | 11 | 113 | 37 | 139 | 1.51e-28 | 107 |
| MS.gene022331.t1 | AT3G47870 | 42.000 | 100 | 58 | 0 | 11 | 110 | 37 | 136 | 9.01e-25 | 99.0 |
| MS.gene022331.t1 | AT1G72980 | 44.792 | 96 | 53 | 0 | 9 | 104 | 12 | 107 | 8.50e-23 | 91.3 |
| MS.gene022331.t1 | AT4G22700 | 37.984 | 129 | 76 | 3 | 11 | 136 | 6 | 133 | 2.36e-21 | 87.0 |
| MS.gene022331.t1 | AT1G06280 | 42.500 | 80 | 46 | 0 | 8 | 87 | 22 | 101 | 5.30e-18 | 78.6 |
| MS.gene022331.t1 | AT5G15060 | 40.260 | 77 | 44 | 1 | 7 | 81 | 14 | 90 | 5.41e-15 | 68.9 |
| MS.gene022331.t1 | AT2G19820 | 36.667 | 90 | 56 | 1 | 9 | 97 | 11 | 100 | 5.79e-15 | 68.6 |
| MS.gene022331.t1 | AT3G27940 | 39.394 | 66 | 40 | 0 | 10 | 75 | 5 | 70 | 2.40e-11 | 59.7 |
| MS.gene022331.t1 | AT3G27940 | 39.394 | 66 | 40 | 0 | 10 | 75 | 5 | 70 | 2.40e-11 | 59.7 |
| MS.gene022331.t1 | AT3G27940 | 39.394 | 66 | 40 | 0 | 10 | 75 | 5 | 70 | 2.40e-11 | 59.7 |
Find 47 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCAACGTTCACAAGATCTT+TGG | 0.310163 | 3.4:+74172723 | MS.gene022331:CDS |
| TCTTCCTCCTCTTGTTGTTC+CGG | 0.318724 | 3.4:+74172983 | MS.gene022331:CDS |
| AGAGGTGGAAGAGAAGAATT+AGG | 0.321024 | 3.4:-74172963 | None:intergenic |
| AGACAGTTTAGTTCAAGATT+TGG | 0.321989 | 3.4:+74173026 | MS.gene022331:CDS |
| GGTTCTTCTGGTGGAAAGTA+TGG | 0.385993 | 3.4:-74172690 | None:intergenic |
| TTTGGTAATGGAAATGTTGA+TGG | 0.387117 | 3.4:+74173044 | MS.gene022331:CDS |
| AATGGAAATGTTGATGGAAA+TGG | 0.392662 | 3.4:+74173050 | MS.gene022331:CDS |
| GGAGGAAGAGGAGGAGGTGT+AGG | 0.401282 | 3.4:+74173146 | MS.gene022331:CDS |
| GGTGTAGGAGGAAGAGGAGG+AGG | 0.413675 | 3.4:+74173140 | MS.gene022331:CDS |
| GGAAGAGGAGGAGGTGTAGG+AGG | 0.430574 | 3.4:+74173149 | MS.gene022331:CDS |
| AGTGGAGGTGTAGGAGGAAG+AGG | 0.435749 | 3.4:+74173134 | MS.gene022331:CDS |
| GATATAAGTGGAGGTGTAGG+AGG | 0.445011 | 3.4:+74173128 | MS.gene022331:CDS |
| AGAGGAGGAGGTGTAGGAGG+AGG | 0.445819 | 3.4:+74173152 | MS.gene022331:CDS |
| TTTAGTTCAAGATTTGGTAA+TGG | 0.446442 | 3.4:+74173032 | MS.gene022331:CDS |
| CGTGTGAGAGATCCTGTTTA+TGG | 0.449251 | 3.4:+74172834 | MS.gene022331:CDS |
| AATTCACTGCATCTTCTCTC+TGG | 0.451599 | 3.4:-74172788 | None:intergenic |
| TTAATCCATGGAATAGCATA+AGG | 0.469520 | 3.4:-74173089 | None:intergenic |
| GCACCAACACAACCATAAAC+AGG | 0.472967 | 3.4:-74172846 | None:intergenic |
| GAGGATATAAGTGGAGGTGT+AGG | 0.488241 | 3.4:+74173125 | MS.gene022331:CDS |
| CTTCGCCGAAAATGCATGCC+AGG | 0.496124 | 3.4:+74172654 | MS.gene022331:CDS |
| CTGCATCTTCTCTCTGGTGA+GGG | 0.502538 | 3.4:-74172782 | None:intergenic |
| ACAAGTTCAGAGGCTTCAAA+AGG | 0.506762 | 3.4:+74172887 | MS.gene022331:CDS |
| GCCTCTGAACTTGTCTCTGA+AGG | 0.518925 | 3.4:-74172878 | None:intergenic |
| GATCCTGTTTATGGTTGTGT+TGG | 0.519160 | 3.4:+74172843 | MS.gene022331:CDS |
| GGAGGTGTAGGAGGAAGAGG+AGG | 0.524745 | 3.4:+74173137 | MS.gene022331:CDS |
| GGAACAACAAGAGGAGGAAG+AGG | 0.530679 | 3.4:-74172981 | None:intergenic |
| ACCTCTTCTGAGGATATAAG+TGG | 0.541161 | 3.4:+74173116 | MS.gene022331:CDS |
| CATCTTCTCTCTGGTGAGGG+AGG | 0.545055 | 3.4:-74172779 | None:intergenic |
| AGGAATTTGCAGGCTGCACA+TGG | 0.545835 | 3.4:-74172633 | None:intergenic |
| ACTGCATCTTCTCTCTGGTG+AGG | 0.556115 | 3.4:-74172783 | None:intergenic |
| TGAGATTGATGAAACGAAGC+CGG | 0.556305 | 3.4:-74173002 | None:intergenic |
| TCCACTTATATCCTCAGAAG+AGG | 0.570244 | 3.4:-74173117 | None:intergenic |
| ACAACAAGAGGAGGAAGAGG+TGG | 0.575947 | 3.4:-74172978 | None:intergenic |
| TCCTTCAGAGACAAGTTCAG+AGG | 0.577279 | 3.4:+74172877 | MS.gene022331:CDS |
| GATTAATGATACCTCTTCTG+AGG | 0.582743 | 3.4:+74173106 | MS.gene022331:CDS |
| AACGAAGCCGGAACAACAAG+AGG | 0.585009 | 3.4:-74172990 | None:intergenic |
| TATGGTGCAAAGATGCAGCC+TGG | 0.588900 | 3.4:-74172672 | None:intergenic |
| TTCCCTTGCTTATGAAGCCG+AGG | 0.588970 | 3.4:+74172809 | MS.gene022331:CDS |
| GCCAAAGATCTTGTGAACGT+TGG | 0.589226 | 3.4:-74172724 | None:intergenic |
| AGGATCTCTCACACGTGCCT+CGG | 0.589439 | 3.4:-74172826 | None:intergenic |
| GAAGAGGTATCATTAATCCA+TGG | 0.590052 | 3.4:-74173101 | None:intergenic |
| GTGCCTCGGCTTCATAAGCA+AGG | 0.591344 | 3.4:-74172812 | None:intergenic |
| GAAGCCGGAACAACAAGAGG+AGG | 0.613964 | 3.4:-74172987 | None:intergenic |
| TTGTCTCTGAAGGAAAGAGA+TGG | 0.626573 | 3.4:-74172868 | None:intergenic |
| TGCCTCGGCTTCATAAGCAA+GGG | 0.648971 | 3.4:-74172811 | None:intergenic |
| GGTGAGGGAGGAGTTCGTTG+AGG | 0.665155 | 3.4:-74172767 | None:intergenic |
| TCTTCTGAGGATATAAGTGG+AGG | 0.670110 | 3.4:+74173119 | MS.gene022331:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAGTTCAAGATTTGGTAA+TGG | + | chr3.4:74173032-74173051 | MS.gene022331:CDS | 25.0% |
| TTAATCCATGGAATAGCATA+AGG | - | chr3.4:74173092-74173111 | None:intergenic | 30.0% | |
| ! | AATGGAAATGTTGATGGAAA+TGG | + | chr3.4:74173050-74173069 | MS.gene022331:CDS | 30.0% |
| ! | AGACAGTTTAGTTCAAGATT+TGG | + | chr3.4:74173026-74173045 | MS.gene022331:CDS | 30.0% |
| !! | TTTGGTAATGGAAATGTTGA+TGG | + | chr3.4:74173044-74173063 | MS.gene022331:CDS | 30.0% |
| GATTAATGATACCTCTTCTG+AGG | + | chr3.4:74173106-74173125 | MS.gene022331:CDS | 35.0% | |
| ! | CTTTTCCTTATGCTATTCCA+TGG | + | chr3.4:74173084-74173103 | MS.gene022331:CDS | 35.0% |
| ! | TGAACGTTGGCAAATTTTTG+AGG | - | chr3.4:74172714-74172733 | None:intergenic | 35.0% |
| !! | GAAGAGGTATCATTAATCCA+TGG | - | chr3.4:74173104-74173123 | None:intergenic | 35.0% |
| !!! | AATTTTTGAGGTTCTTCTGG+TGG | - | chr3.4:74172702-74172721 | None:intergenic | 35.0% |
| !!! | GCAAATTTTTGAGGTTCTTC+TGG | - | chr3.4:74172705-74172724 | None:intergenic | 35.0% |
| AATTCACTGCATCTTCTCTC+TGG | - | chr3.4:74172791-74172810 | None:intergenic | 40.0% | |
| ACAAGTTCAGAGGCTTCAAA+AGG | + | chr3.4:74172887-74172906 | MS.gene022331:CDS | 40.0% | |
| AGAGGTGGAAGAGAAGAATT+AGG | - | chr3.4:74172966-74172985 | None:intergenic | 40.0% | |
| GATCCTGTTTATGGTTGTGT+TGG | + | chr3.4:74172843-74172862 | MS.gene022331:CDS | 40.0% | |
| TCCACTTATATCCTCAGAAG+AGG | - | chr3.4:74173120-74173139 | None:intergenic | 40.0% | |
| TGAGATTGATGAAACGAAGC+CGG | - | chr3.4:74173005-74173024 | None:intergenic | 40.0% | |
| ! | ACCTCTTCTGAGGATATAAG+TGG | + | chr3.4:74173116-74173135 | MS.gene022331:CDS | 40.0% |
| ! | TCTTCTGAGGATATAAGTGG+AGG | + | chr3.4:74173119-74173138 | MS.gene022331:CDS | 40.0% |
| ! | TTGTCTCTGAAGGAAAGAGA+TGG | - | chr3.4:74172871-74172890 | None:intergenic | 40.0% |
| CGTGTGAGAGATCCTGTTTA+TGG | + | chr3.4:74172834-74172853 | MS.gene022331:CDS | 45.0% | |
| GCACCAACACAACCATAAAC+AGG | - | chr3.4:74172849-74172868 | None:intergenic | 45.0% | |
| GCCAAAGATCTTGTGAACGT+TGG | - | chr3.4:74172727-74172746 | None:intergenic | 45.0% | |
| GCCAACGTTCACAAGATCTT+TGG | + | chr3.4:74172723-74172742 | MS.gene022331:CDS | 45.0% | |
| GGTTCTTCTGGTGGAAAGTA+TGG | - | chr3.4:74172693-74172712 | None:intergenic | 45.0% | |
| TCCTTCAGAGACAAGTTCAG+AGG | + | chr3.4:74172877-74172896 | MS.gene022331:CDS | 45.0% | |
| ! | GAGGATATAAGTGGAGGTGT+AGG | + | chr3.4:74173125-74173144 | MS.gene022331:CDS | 45.0% |
| ! | GATATAAGTGGAGGTGTAGG+AGG | + | chr3.4:74173128-74173147 | MS.gene022331:CDS | 45.0% |
| ! | TTTTCGGCGAAGGAATTTGC+AGG | - | chr3.4:74172646-74172665 | None:intergenic | 45.0% |
| !! | TCTTCCTCCTCTTGTTGTTC+CGG | + | chr3.4:74172983-74173002 | MS.gene022331:CDS | 45.0% |
| AACGAAGCCGGAACAACAAG+AGG | - | chr3.4:74172993-74173012 | None:intergenic | 50.0% | |
| ACAACAAGAGGAGGAAGAGG+TGG | - | chr3.4:74172981-74173000 | None:intergenic | 50.0% | |
| ACTGCATCTTCTCTCTGGTG+AGG | - | chr3.4:74172786-74172805 | None:intergenic | 50.0% | |
| AGGAATTTGCAGGCTGCACA+TGG | - | chr3.4:74172636-74172655 | None:intergenic | 50.0% | |
| CTGCATCTTCTCTCTGGTGA+GGG | - | chr3.4:74172785-74172804 | None:intergenic | 50.0% | |
| GCCTCTGAACTTGTCTCTGA+AGG | - | chr3.4:74172881-74172900 | None:intergenic | 50.0% | |
| GGAACAACAAGAGGAGGAAG+AGG | - | chr3.4:74172984-74173003 | None:intergenic | 50.0% | |
| TATGGTGCAAAGATGCAGCC+TGG | - | chr3.4:74172675-74172694 | None:intergenic | 50.0% | |
| TGCCTCGGCTTCATAAGCAA+GGG | - | chr3.4:74172814-74172833 | None:intergenic | 50.0% | |
| TTCCCTTGCTTATGAAGCCG+AGG | + | chr3.4:74172809-74172828 | MS.gene022331:CDS | 50.0% | |
| ! | TGCAGCCTGGCATGCATTTT+CGG | - | chr3.4:74172662-74172681 | None:intergenic | 50.0% |
| AGGATCTCTCACACGTGCCT+CGG | - | chr3.4:74172829-74172848 | None:intergenic | 55.0% | |
| CATCTTCTCTCTGGTGAGGG+AGG | - | chr3.4:74172782-74172801 | None:intergenic | 55.0% | |
| CTTCGCCGAAAATGCATGCC+AGG | + | chr3.4:74172654-74172673 | MS.gene022331:CDS | 55.0% | |
| GAAGCCGGAACAACAAGAGG+AGG | - | chr3.4:74172990-74173009 | None:intergenic | 55.0% | |
| GTGCCTCGGCTTCATAAGCA+AGG | - | chr3.4:74172815-74172834 | None:intergenic | 55.0% | |
| ! | AGTGGAGGTGTAGGAGGAAG+AGG | + | chr3.4:74173134-74173153 | MS.gene022331:CDS | 55.0% |
| ! | CTGGCATGCATTTTCGGCGA+AGG | - | chr3.4:74172656-74172675 | None:intergenic | 55.0% |
| GGTGAGGGAGGAGTTCGTTG+AGG | - | chr3.4:74172770-74172789 | None:intergenic | 60.0% | |
| GGTGTAGGAGGAAGAGGAGG+AGG | + | chr3.4:74173140-74173159 | MS.gene022331:CDS | 60.0% | |
| ! | AGAGGAGGAGGTGTAGGAGG+AGG | + | chr3.4:74173152-74173171 | MS.gene022331:CDS | 60.0% |
| ! | GGAAGAGGAGGAGGTGTAGG+AGG | + | chr3.4:74173149-74173168 | MS.gene022331:CDS | 60.0% |
| ! | GGAGGAAGAGGAGGAGGTGT+AGG | + | chr3.4:74173146-74173165 | MS.gene022331:CDS | 60.0% |
| ! | GGAGGTGTAGGAGGAAGAGG+AGG | + | chr3.4:74173137-74173156 | MS.gene022331:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 74172606 | 74173187 | 74172606 | ID=MS.gene022331 |
| chr3.4 | mRNA | 74172606 | 74173187 | 74172606 | ID=MS.gene022331.t1;Parent=MS.gene022331 |
| chr3.4 | exon | 74172606 | 74173187 | 74172606 | ID=MS.gene022331.t1.exon1;Parent=MS.gene022331.t1 |
| chr3.4 | CDS | 74172606 | 74173187 | 74172606 | ID=cds.MS.gene022331.t1;Parent=MS.gene022331.t1 |
| Gene Sequence |
| Protein sequence |
