AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022419

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022419.t1 MTR_3g075270 91.531 307 26 0 48 354 1 307 0.0 572
MS.gene022419.t1 MTR_4g104140 50.909 330 151 4 9 328 7 335 5.16e-119 348
MS.gene022419.t1 MTR_4g104140 56.078 255 109 2 77 328 11 265 9.57e-101 299
MS.gene022419.t1 MTR_5g015680 37.458 299 179 3 17 308 66 363 1.50e-69 222
MS.gene022419.t1 MTR_5g015680 37.458 299 179 3 17 308 105 402 6.60e-69 222
MS.gene022419.t1 MTR_5g015680 38.806 268 161 2 44 308 1 268 1.20e-65 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022419.t1 AT3G48360 46.557 305 142 4 31 314 46 350 2.41e-88 270
MS.gene022419.t1 AT5G63160 46.224 331 157 6 26 338 32 359 8.39e-87 266
MS.gene022419.t1 AT3G48360 47.232 271 123 3 31 281 46 316 4.94e-79 245
MS.gene022419.t1 AT5G63160 47.253 273 127 4 26 281 32 304 1.62e-78 243
MS.gene022419.t1 AT3G48360 50.000 244 111 3 82 314 1 244 9.72e-75 232
MS.gene022419.t1 AT5G63160 49.259 270 125 5 78 338 8 274 1.16e-73 230
MS.gene022419.t1 AT1G05690 38.614 303 174 6 31 325 66 364 5.58e-65 210
MS.gene022419.t1 AT5G67480 37.209 301 178 6 17 308 59 357 3.34e-62 203
MS.gene022419.t1 AT5G67480 37.209 301 178 6 17 308 59 357 3.34e-62 203
MS.gene022419.t1 AT5G67480 37.209 301 178 6 17 308 70 368 3.56e-62 203
MS.gene022419.t1 AT4G37610 36.913 298 176 7 20 308 57 351 2.57e-61 201

Find 92 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACCGCTTCAAAATTAT+TGG 0.124222 3.2:+67974808 None:intergenic
CTCCACATGTCAAGCTTTGC+AGG 0.259755 3.2:-67974465 MS.gene022419:CDS
ATGGATATAAATGTAAGTTT+TGG 0.269976 3.2:+67975496 None:intergenic
AATGCACCTTTGCTTTAGTT+TGG 0.301923 3.2:+67974937 None:intergenic
CATGCAAGGTTCCTCTTTGC+AGG 0.308788 3.2:-67974328 MS.gene022419:intron
TACATTTCATCTACACTTCC+AGG 0.332926 3.2:-67975237 MS.gene022419:intron
GTATTATGCCACTGGGAGTA+TGG 0.339417 3.2:+67975477 None:intergenic
CATTCCCCGTGGTCATGAAT+TGG 0.355331 3.2:+67974903 None:intergenic
ATCATATGATGTGGAGATTA+CGG 0.355897 3.2:-67974507 MS.gene022419:CDS
TCCAGTGCACGCAACCGTTC+TGG 0.358145 3.2:-67975450 MS.gene022419:intron
AAGTGAAGCTGTACAATGTT+TGG 0.366740 3.2:-67974564 MS.gene022419:CDS
ATGCACCTTTGCTTTAGTTT+GGG 0.372763 3.2:+67974938 None:intergenic
ACCAGAACGGTTGCGTGCAC+TGG 0.385474 3.2:+67975449 None:intergenic
ACACACTTGAAGTAGAGATT+TGG 0.400686 3.2:+67974842 None:intergenic
AACACTTTACCACATGTAAT+AGG 0.401202 3.2:-67974433 MS.gene022419:CDS
AGTCAAGAGAGTCTTCTAAT+TGG 0.402862 3.2:-67973551 MS.gene022419:CDS
TCATATGATGTGGAGATTAC+GGG 0.403872 3.2:-67974506 MS.gene022419:CDS
TGGGACTATGCAGGTGCAGC+AGG 0.411993 3.2:-67975018 MS.gene022419:intron
CCTTGTCCTTAGCAATTGTA+AGG 0.421710 3.2:+67973620 None:intergenic
GTCCCCAATTCTAAAAGAAA+TGG 0.422817 3.2:-67975320 MS.gene022419:CDS
CAAGGAGAGATGAGGTAATT+CGG 0.427024 3.2:-67973602 MS.gene022419:CDS
GAGGAACCTTGCATGCATCT+TGG 0.458524 3.2:+67974336 None:intergenic
GAAGAGAGGAAATAAACATA+GGG 0.473780 3.2:-67974612 MS.gene022419:CDS
AGAAGAGAGGAAATAAACAT+AGG 0.475467 3.2:-67974613 MS.gene022419:CDS
AACGGTTGCGTGCACTGGAA+AGG 0.491203 3.2:+67975454 None:intergenic
GAATACTAGAGTAGAGGAAG+AGG 0.495047 3.2:-67973580 MS.gene022419:CDS
TGGAAAGGGTATTATGCCAC+TGG 0.495282 3.2:+67975469 None:intergenic
ACGGTTGCGTGCACTGGAAA+GGG 0.504977 3.2:+67975455 None:intergenic
CAAGAGAGTCTTCTAATTGG+TGG 0.514114 3.2:-67973548 MS.gene022419:CDS
AACGGTAACGTTAAAATATG+TGG 0.514946 3.2:-67975289 MS.gene022419:CDS
AAGGCGATGGCTGCATTGGT+TGG 0.517717 3.2:+67975262 None:intergenic
TCCACATGTCAAGCTTTGCA+GGG 0.524594 3.2:-67974464 MS.gene022419:CDS
TTGATCCAATTCATGACCAC+GGG 0.542240 3.2:-67974908 MS.gene022419:CDS
TATTGCATAAACATGACCCT+TGG 0.542900 3.2:-67974766 MS.gene022419:CDS
TACGAGGTGGAAACAAATAG+TGG 0.546964 3.2:-67973673 MS.gene022419:CDS
TACAAAGGCGATGGCTGCAT+TGG 0.548314 3.2:+67975258 None:intergenic
CTTTAACCAAGATGCATGCA+AGG 0.552171 3.2:-67974342 MS.gene022419:CDS
CCTTACAATTGCTAAGGACA+AGG 0.552243 3.2:-67973620 MS.gene022419:CDS
TCTCAACAAAGAACGAGAAT+CGG 0.559033 3.2:-67974723 MS.gene022419:intron
ATTCTCGTTCTTTGTTGAGA+CGG 0.562147 3.2:+67974726 None:intergenic
TGTCTTCCTTACAATTGCTA+AGG 0.563853 3.2:-67973626 MS.gene022419:CDS
CCACATGTCAAGCTTTGCAG+GGG 0.564441 3.2:-67974463 MS.gene022419:CDS
GATAGCTGAGAAAGAGAACA+AGG 0.575893 3.2:-67973703 MS.gene022419:CDS
TTGGAGCATATATGTAGAGA+AGG 0.575989 3.2:-67974545 MS.gene022419:CDS
ATGATGTCAACTTCGAGCCA+AGG 0.576653 3.2:+67974749 None:intergenic
ATCTTGGTTAAAGCAAATGC+AGG 0.576779 3.2:+67974352 None:intergenic
ATTCATGACCACGGGGAATG+TGG 0.583872 3.2:-67974900 MS.gene022419:CDS
TGTAATAGGAGACTCGAGAG+AGG 0.587137 3.2:-67974419 MS.gene022419:CDS
TGATGTCAACTTCGAGCCAA+GGG 0.587603 3.2:+67974750 None:intergenic
AAAGAAATGGTAACGTCCAA+CGG 0.587929 3.2:-67975307 MS.gene022419:CDS
ACGAGGTGGAAACAAATAGT+GGG 0.591901 3.2:-67973672 MS.gene022419:CDS
TGTGGTGGATGTGTTGCACT+TGG 0.594113 3.2:-67974882 MS.gene022419:CDS
CCCCTGCAAAGCTTGACATG+TGG 0.598401 3.2:+67974463 None:intergenic
TGTTTCGCATACCTGCAAAG+AGG 0.600432 3.2:+67974317 None:intergenic
CAATGCCCAAACTAAAGCAA+AGG 0.601233 3.2:-67974943 MS.gene022419:CDS
TTGTAAGGAAGACATGGCTA+TGG 0.601532 3.2:+67973635 None:intergenic
GAAGCGGTGAAGGAAACAGA+GGG 0.603513 3.2:-67974797 MS.gene022419:CDS
GCTGAGAAAGAGAACAAGGA+AGG 0.604375 3.2:-67973699 MS.gene022419:CDS
CAACGTGGCATCATATGATG+TGG 0.607548 3.2:-67974516 MS.gene022419:CDS
AATTCGGAATACTAGAGTAG+AGG 0.608608 3.2:-67973586 MS.gene022419:CDS
CTCGAGTCTCCTATTACATG+TGG 0.612075 3.2:+67974424 None:intergenic
GGAGAAAACAGAGAAGCCAT+TGG 0.615463 3.2:+67975545 None:intergenic
GGAGACTCGAGAGAGGGTGT+AGG 0.617740 3.2:-67974412 MS.gene022419:CDS
GAGAGGAAATAAACATAGGG+AGG 0.621519 3.2:-67974609 MS.gene022419:CDS
CTTGATCCAATTCATGACCA+CGG 0.630679 3.2:-67974909 MS.gene022419:CDS
CATATGATGTGGAGATTACG+GGG 0.632166 3.2:-67974505 MS.gene022419:CDS
TAGCAATTGTAAGGAAGACA+TGG 0.634002 3.2:+67973629 None:intergenic
GTAGGTGTTGTAAGTCTATG+TGG 0.636359 3.2:-67974394 MS.gene022419:CDS
GTGAAACAGAGGAAGAAGAG+AGG 0.641073 3.2:-67974626 MS.gene022419:intron
CGGTGAAGGAAACAGAGGGG+TGG 0.647649 3.2:-67974793 MS.gene022419:CDS
AGTGTAGATGAAATGTACAA+AGG 0.647924 3.2:+67975243 None:intergenic
GGAAAGGGTATTATGCCACT+GGG 0.651960 3.2:+67975470 None:intergenic
GCTAAGGACAAGGAGAGATG+AGG 0.655281 3.2:-67973610 MS.gene022419:CDS
AAAATAGAAGAGTACCAGAA+CGG 0.658371 3.2:+67975436 None:intergenic
GGGACTATGCAGGTGCAGCA+GGG 0.659269 3.2:-67975017 MS.gene022419:intron
ACAAGGAAGGTGATACGAGG+TGG 0.662953 3.2:-67973686 MS.gene022419:CDS
CATGTGGAGAATTTAGTACA+CGG 0.664071 3.2:+67974479 None:intergenic
CACATCATATGATGCCACGT+TGG 0.666948 3.2:+67974517 None:intergenic
GATGAAATGTACAAAGGCGA+TGG 0.667885 3.2:+67975249 None:intergenic
CATGACCACGGGGAATGTGG+TGG 0.668455 3.2:-67974897 MS.gene022419:CDS
GTAATAGGAGACTCGAGAGA+GGG 0.668774 3.2:-67974418 MS.gene022419:CDS
TGAAGCGGTGAAGGAAACAG+AGG 0.678822 3.2:-67974798 MS.gene022419:CDS
ATTTATATCCATACTCCCAG+TGG 0.683113 3.2:-67975485 MS.gene022419:CDS
CGAGGTGGAAACAAATAGTG+GGG 0.698764 3.2:-67973671 MS.gene022419:CDS
TAGAGAAGGTTGCACCAACG+TGG 0.699752 3.2:-67974531 MS.gene022419:CDS
CACATCCACCACATTCCCCG+TGG 0.715239 3.2:+67974892 None:intergenic
AGACGTGCGAGAGTACGAGA+AGG 0.721408 3.2:+67974969 None:intergenic
ACTATGCAGGTGCAGCAGGG+AGG 0.723689 3.2:-67975014 MS.gene022419:intron
AGAACAAGGAAGGTGATACG+AGG 0.743284 3.2:-67973689 MS.gene022419:CDS
ATGATGTGGAGATTACGGGG+AGG 0.765492 3.2:-67974502 MS.gene022419:CDS
AAGCGGTGAAGGAAACAGAG+GGG 0.769201 3.2:-67974796 MS.gene022419:CDS
TGATCCAATTCATGACCACG+GGG 0.774666 3.2:-67974907 MS.gene022419:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGAATTAACAAATTTTT+TGG - chr3.2:67973891-67973910 MS.gene022419:intron 10.0%
!!! ACATTTTTTCATATAAAAAA+AGG - chr3.2:67974790-67974809 MS.gene022419:CDS 10.0%
!! TATATATTTCTTTAGTAAGT+TGG - chr3.2:67974401-67974420 MS.gene022419:CDS 15.0%
!!! GTTATTAATCGTTATTATTT+TGG - chr3.2:67974890-67974909 MS.gene022419:CDS 15.0%
!! ATATAGTGATTGAAAATGAA+CGG + chr3.2:67973658-67973677 None:intergenic 20.0%
!! GGTTAATAAAGCTATATTTA+CGG - chr3.2:67975308-67975327 MS.gene022419:CDS 20.0%
!!! ATGGATATAAATGTAAGTTT+TGG + chr3.2:67973564-67973583 None:intergenic 20.0%
!!! TAGTTACTAGTTTTCATTTA+GGG - chr3.2:67975280-67975299 MS.gene022419:CDS 20.0%
!!! TGGATATAAATGTAAGTTTT+GGG + chr3.2:67973563-67973582 None:intergenic 20.0%
!!! TTACCATTTCTTTTAGAATT+GGG + chr3.2:67973743-67973762 None:intergenic 20.0%
! AAACAAATAATAACTACAGC+TGG - chr3.2:67974815-67974834 MS.gene022419:CDS 25.0%
! TGACTAATTGAGGAATATAA+TGG - chr3.2:67974988-67975007 MS.gene022419:CDS 25.0%
!! GGATATAAATGTAAGTTTTG+GGG + chr3.2:67973562-67973581 None:intergenic 25.0%
!! GGTCTAAATACATCAATTTT+CGG - chr3.2:67973912-67973931 MS.gene022419:intron 25.0%
!!! AGTTACCAATAATTTTGAAG+CGG - chr3.2:67974244-67974263 MS.gene022419:intron 25.0%
!!! CTAGTTACTAGTTTTCATTT+AGG - chr3.2:67975279-67975298 MS.gene022419:CDS 25.0%
!!! CTTTGAAATTAACCATCAAT+GGG - chr3.2:67975059-67975078 MS.gene022419:intron 25.0%
!!! GATATAAATGTAAGTTTTGG+GGG + chr3.2:67973561-67973580 None:intergenic 25.0%
!!! GTTACCATTTCTTTTAGAAT+TGG + chr3.2:67973744-67973763 None:intergenic 25.0%
!!! TACCATTTCTTTTAGAATTG+GGG + chr3.2:67973742-67973761 None:intergenic 25.0%
!!! TAGTTTTCATTTAGGGTATT+TGG - chr3.2:67975287-67975306 MS.gene022419:CDS 25.0%
AACACTTTACCACATGTAAT+AGG - chr3.2:67974624-67974643 MS.gene022419:intron 30.0%
AACGGTAACGTTAAAATATG+TGG - chr3.2:67973768-67973787 MS.gene022419:intron 30.0%
AAGTAGCAATTGACTAATTG+AGG - chr3.2:67974978-67974997 MS.gene022419:CDS 30.0%
AGAAGAGAGGAAATAAACAT+AGG - chr3.2:67974444-67974463 MS.gene022419:CDS 30.0%
AGGGAGGATATGAAAAATTA+TGG - chr3.2:67974059-67974078 MS.gene022419:intron 30.0%
AGTGTAGATGAAATGTACAA+AGG + chr3.2:67973817-67973836 None:intergenic 30.0%
ATAGTCCCAATCAATTCTTA+GGG + chr3.2:67974028-67974047 None:intergenic 30.0%
ATCATATGATGTGGAGATTA+CGG - chr3.2:67974550-67974569 MS.gene022419:CDS 30.0%
CACTTGACAAAAAAAGACAA+TGG - chr3.2:67973999-67974018 MS.gene022419:intron 30.0%
GAAGAGAGGAAATAAACATA+GGG - chr3.2:67974445-67974464 MS.gene022419:CDS 30.0%
GACTAACTCACTAAAATGTT+TGG + chr3.2:67975232-67975251 None:intergenic 30.0%
TAATTTCACTATTACTGACC+TGG + chr3.2:67973841-67973860 None:intergenic 30.0%
TATATTTACGGAAATGTGAC+AGG - chr3.2:67975320-67975339 MS.gene022419:CDS 30.0%
TTGTATGATATACCCATTGA+TGG + chr3.2:67975074-67975093 None:intergenic 30.0%
! CAAATGCAATTGCATGTTTT+CGG - chr3.2:67975533-67975552 MS.gene022419:CDS 30.0%
! CCATTGATGGTTAATTTCAA+AGG + chr3.2:67975061-67975080 None:intergenic 30.0%
!! AAAATAGAAGAGTACCAGAA+CGG + chr3.2:67973624-67973643 None:intergenic 30.0%
!! CCTTTGAAATTAACCATCAA+TGG - chr3.2:67975058-67975077 MS.gene022419:intron 30.0%
!! CGTTCTAAAATTGATCTCAA+AGG - chr3.2:67973707-67973726 MS.gene022419:CDS 30.0%
!! GGCATATTTTTACATTCGTT+GGG - chr3.2:67975122-67975141 MS.gene022419:intron 30.0%
!! TGGCATATTTTTACATTCGT+TGG - chr3.2:67975121-67975140 MS.gene022419:intron 30.0%
!! TTCTTTAGTAAGTTGGTGAT+CGG - chr3.2:67974408-67974427 MS.gene022419:CDS 30.0%
!!! ACATATTTTAACGTTACCGT+TGG + chr3.2:67973769-67973788 None:intergenic 30.0%
!!! CATTGTCTTTTTTTGTCAAG+TGG + chr3.2:67974001-67974020 None:intergenic 30.0%
AAGTGAAGCTGTACAATGTT+TGG - chr3.2:67974493-67974512 MS.gene022419:CDS 35.0%
AATGCACCTTTGCTTTAGTT+TGG + chr3.2:67974123-67974142 None:intergenic 35.0%
AATTCGGAATACTAGAGTAG+AGG - chr3.2:67975471-67975490 MS.gene022419:CDS 35.0%
ACAAAGTGACAAAACCAACA+CGG - chr3.2:67975148-67975167 MS.gene022419:intron 35.0%
ACACACTTGAAGTAGAGATT+TGG + chr3.2:67974218-67974237 None:intergenic 35.0%
AGTCAAGAGAGTCTTCTAAT+TGG - chr3.2:67975506-67975525 MS.gene022419:CDS 35.0%
ATAATGTATACGTACCGTGT+TGG + chr3.2:67975165-67975184 None:intergenic 35.0%
ATCTTGGTTAAAGCAAATGC+AGG + chr3.2:67974708-67974727 None:intergenic 35.0%
ATTCTCGTTCTTTGTTGAGA+CGG + chr3.2:67974334-67974353 None:intergenic 35.0%
ATTTATATCCATACTCCCAG+TGG - chr3.2:67973572-67973591 MS.gene022419:CDS 35.0%
CACGGTACGTATACATTATA+TGG - chr3.2:67975166-67975185 MS.gene022419:intron 35.0%
CATAGTCCCAATCAATTCTT+AGG + chr3.2:67974029-67974048 None:intergenic 35.0%
CATGTGGAGAATTTAGTACA+CGG + chr3.2:67974581-67974600 None:intergenic 35.0%
CTTCACCGCTTCAAAATTAT+TGG + chr3.2:67974252-67974271 None:intergenic 35.0%
TACATTTCATCTACACTTCC+AGG - chr3.2:67973820-67973839 MS.gene022419:intron 35.0%
TAGCAATTGTAAGGAAGACA+TGG + chr3.2:67975431-67975450 None:intergenic 35.0%
TATTGCATAAACATGACCCT+TGG - chr3.2:67974291-67974310 MS.gene022419:intron 35.0%
TCATATGATGTGGAGATTAC+GGG - chr3.2:67974551-67974570 MS.gene022419:CDS 35.0%
TCTCAACAAAGAACGAGAAT+CGG - chr3.2:67974334-67974353 MS.gene022419:CDS 35.0%
TGTCTTCCTTACAATTGCTA+AGG - chr3.2:67975431-67975450 MS.gene022419:intron 35.0%
TTGGAGCATATATGTAGAGA+AGG - chr3.2:67974512-67974531 MS.gene022419:CDS 35.0%
! AAAGAAATGGTAACGTCCAA+CGG - chr3.2:67973750-67973769 MS.gene022419:intron 35.0%
! ATGCACCTTTGCTTTAGTTT+GGG + chr3.2:67974122-67974141 None:intergenic 35.0%
! GTCCCCAATTCTAAAAGAAA+TGG - chr3.2:67973737-67973756 MS.gene022419:intron 35.0%
! TTTTTGTCAAGTGGATGAGT+GGG + chr3.2:67973992-67974011 None:intergenic 35.0%
!! TCTTTAGTAAGTTGGTGATC+GGG - chr3.2:67974409-67974428 MS.gene022419:CDS 35.0%
!!! CAATAATTTTGAAGCGGTGA+AGG - chr3.2:67974250-67974269 MS.gene022419:intron 35.0%
!!! TTTTTTGTCAAGTGGATGAG+TGG + chr3.2:67973993-67974012 None:intergenic 35.0%
ACGAGGTGGAAACAAATAGT+GGG - chr3.2:67975385-67975404 MS.gene022419:intron 40.0%
AGAATTGATTGGGACTATGC+AGG - chr3.2:67974030-67974049 MS.gene022419:intron 40.0%
CAAGAGAGTCTTCTAATTGG+TGG - chr3.2:67975509-67975528 MS.gene022419:CDS 40.0%
CAAGGAGAGATGAGGTAATT+CGG - chr3.2:67975455-67975474 MS.gene022419:CDS 40.0%
CAATGCCCAAACTAAAGCAA+AGG - chr3.2:67974114-67974133 MS.gene022419:intron 40.0%
CATATGATGTGGAGATTACG+GGG - chr3.2:67974552-67974571 MS.gene022419:CDS 40.0%
CATGTAGCTAACTACTAACG+TGG - chr3.2:67975101-67975120 MS.gene022419:intron 40.0%
CCTTACAATTGCTAAGGACA+AGG - chr3.2:67975437-67975456 MS.gene022419:intron 40.0%
CCTTGTCCTTAGCAATTGTA+AGG + chr3.2:67975440-67975459 None:intergenic 40.0%
CTTGATCCAATTCATGACCA+CGG - chr3.2:67974148-67974167 MS.gene022419:intron 40.0%
CTTTAACCAAGATGCATGCA+AGG - chr3.2:67974715-67974734 MS.gene022419:intron 40.0%
GAAAATGAACGGAGACATGA+AGG + chr3.2:67973647-67973666 None:intergenic 40.0%
GAATACTAGAGTAGAGGAAG+AGG - chr3.2:67975477-67975496 MS.gene022419:CDS 40.0%
GAGAGGAAATAAACATAGGG+AGG - chr3.2:67974448-67974467 MS.gene022419:CDS 40.0%
GATAGCTGAGAAAGAGAACA+AGG - chr3.2:67975354-67975373 MS.gene022419:intron 40.0%
GATGAAATGTACAAAGGCGA+TGG + chr3.2:67973811-67973830 None:intergenic 40.0%
GGTGACCCTAAGAATTGATT+GGG - chr3.2:67974020-67974039 MS.gene022419:intron 40.0%
TACGAGGTGGAAACAAATAG+TGG - chr3.2:67975384-67975403 MS.gene022419:intron 40.0%
TGGTGACCCTAAGAATTGAT+TGG - chr3.2:67974019-67974038 MS.gene022419:intron 40.0%
TTGATCCAATTCATGACCAC+GGG - chr3.2:67974149-67974168 MS.gene022419:intron 40.0%
TTGTAAGGAAGACATGGCTA+TGG + chr3.2:67975425-67975444 None:intergenic 40.0%
! GTAGGTGTTGTAAGTCTATG+TGG - chr3.2:67974663-67974682 MS.gene022419:intron 40.0%
!!! TGTAAGTTTTGGGGGAAAAG+CGG + chr3.2:67973553-67973572 None:intergenic 40.0%
AGAACAAGGAAGGTGATACG+AGG - chr3.2:67975368-67975387 MS.gene022419:intron 45.0%
ATGATGTCAACTTCGAGCCA+AGG + chr3.2:67974311-67974330 None:intergenic 45.0%
CAACGTGGCATCATATGATG+TGG - chr3.2:67974541-67974560 MS.gene022419:CDS 45.0%
CACATCATATGATGCCACGT+TGG + chr3.2:67974543-67974562 None:intergenic 45.0%
CGAGGTGGAAACAAATAGTG+GGG - chr3.2:67975386-67975405 MS.gene022419:intron 45.0%
CTCGAGTCTCCTATTACATG+TGG + chr3.2:67974636-67974655 None:intergenic 45.0%
GCTGAGAAAGAGAACAAGGA+AGG - chr3.2:67975358-67975377 MS.gene022419:intron 45.0%
GTAATAGGAGACTCGAGAGA+GGG - chr3.2:67974639-67974658 MS.gene022419:intron 45.0%
GTATTATGCCACTGGGAGTA+TGG + chr3.2:67973583-67973602 None:intergenic 45.0%
GTGAAACAGAGGAAGAAGAG+AGG - chr3.2:67974431-67974450 MS.gene022419:CDS 45.0%
TCCACATGTCAAGCTTTGCA+GGG - chr3.2:67974593-67974612 MS.gene022419:CDS 45.0%
TGATCCAATTCATGACCACG+GGG - chr3.2:67974150-67974169 MS.gene022419:intron 45.0%
TGATGTCAACTTCGAGCCAA+GGG + chr3.2:67974310-67974329 None:intergenic 45.0%
TGTAATAGGAGACTCGAGAG+AGG - chr3.2:67974638-67974657 MS.gene022419:intron 45.0%
TGTTTCGCATACCTGCAAAG+AGG + chr3.2:67974743-67974762 None:intergenic 45.0%
!! GGAAAGGGTATTATGCCACT+GGG + chr3.2:67973590-67973609 None:intergenic 45.0%
!! TGGAAAGGGTATTATGCCAC+TGG + chr3.2:67973591-67973610 None:intergenic 45.0%
!!! GTAAGTTTTGGGGGAAAAGC+GGG + chr3.2:67973552-67973571 None:intergenic 45.0%
AAGCGGTGAAGGAAACAGAG+GGG - chr3.2:67974261-67974280 MS.gene022419:intron 50.0%
ACAAGGAAGGTGATACGAGG+TGG - chr3.2:67975371-67975390 MS.gene022419:intron 50.0%
ATGATGTGGAGATTACGGGG+AGG - chr3.2:67974555-67974574 MS.gene022419:CDS 50.0%
ATTCATGACCACGGGGAATG+TGG - chr3.2:67974157-67974176 MS.gene022419:intron 50.0%
CATGCAAGGTTCCTCTTTGC+AGG - chr3.2:67974729-67974748 MS.gene022419:CDS 50.0%
CATTCCCCGTGGTCATGAAT+TGG + chr3.2:67974157-67974176 None:intergenic 50.0%
CCACATGTCAAGCTTTGCAG+GGG - chr3.2:67974594-67974613 MS.gene022419:CDS 50.0%
CTCCACATGTCAAGCTTTGC+AGG - chr3.2:67974592-67974611 MS.gene022419:CDS 50.0%
GAAGCGGTGAAGGAAACAGA+GGG - chr3.2:67974260-67974279 MS.gene022419:intron 50.0%
GAGGAACCTTGCATGCATCT+TGG + chr3.2:67974724-67974743 None:intergenic 50.0%
GCTAAGGACAAGGAGAGATG+AGG - chr3.2:67975447-67975466 MS.gene022419:intron 50.0%
TACAAAGGCGATGGCTGCAT+TGG + chr3.2:67973802-67973821 None:intergenic 50.0%
TAGAGAAGGTTGCACCAACG+TGG - chr3.2:67974526-67974545 MS.gene022419:CDS 50.0%
TGAAGCGGTGAAGGAAACAG+AGG - chr3.2:67974259-67974278 MS.gene022419:intron 50.0%
! TTGGTGATCGGGTGAAACAG+AGG - chr3.2:67974420-67974439 MS.gene022419:CDS 50.0%
!! TGTGGTGGATGTGTTGCACT+TGG - chr3.2:67974175-67974194 MS.gene022419:intron 50.0%
AACGGTTGCGTGCACTGGAA+AGG + chr3.2:67973606-67973625 None:intergenic 55.0%
ACGGTTGCGTGCACTGGAAA+GGG + chr3.2:67973605-67973624 None:intergenic 55.0%
CCCCTGCAAAGCTTGACATG+TGG + chr3.2:67974597-67974616 None:intergenic 55.0%
! AAGGCGATGGCTGCATTGGT+TGG + chr3.2:67973798-67973817 None:intergenic 55.0%
! AGACGTGCGAGAGTACGAGA+AGG + chr3.2:67974091-67974110 None:intergenic 55.0%
!! TTTTGGGGGAAAAGCGGGTG+GGG + chr3.2:67973547-67973566 None:intergenic 55.0%
!!! AGTTTTGGGGGAAAAGCGGG+TGG + chr3.2:67973549-67973568 None:intergenic 55.0%
!!! GTTTTGGGGGAAAAGCGGGT+GGG + chr3.2:67973548-67973567 None:intergenic 55.0%
AAAGCGGGTGGGGGAGACAA+TGG + chr3.2:67973537-67973556 None:intergenic 60.0%
AAGCGGGTGGGGGAGACAAT+GGG + chr3.2:67973536-67973555 None:intergenic 60.0%
ACCAGAACGGTTGCGTGCAC+TGG + chr3.2:67973611-67973630 None:intergenic 60.0%
ACTATGCAGGTGCAGCAGGG+AGG - chr3.2:67974043-67974062 MS.gene022419:intron 60.0%
CACATCCACCACATTCCCCG+TGG + chr3.2:67974168-67974187 None:intergenic 60.0%
CATGACCACGGGGAATGTGG+TGG - chr3.2:67974160-67974179 MS.gene022419:intron 60.0%
CGGTGAAGGAAACAGAGGGG+TGG - chr3.2:67974264-67974283 MS.gene022419:intron 60.0%
GGGACTATGCAGGTGCAGCA+GGG - chr3.2:67974040-67974059 MS.gene022419:intron 60.0%
TCCAGTGCACGCAACCGTTC+TGG - chr3.2:67973607-67973626 MS.gene022419:CDS 60.0%
TGGGACTATGCAGGTGCAGC+AGG - chr3.2:67974039-67974058 MS.gene022419:intron 60.0%
TTTGGGGGAAAAGCGGGTGG+GGG + chr3.2:67973546-67973565 None:intergenic 60.0%
! GGAGACTCGAGAGAGGGTGT+AGG - chr3.2:67974645-67974664 MS.gene022419:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 67973515 67975564 67973515 ID=MS.gene022419
chr3.2 mRNA 67973515 67975564 67973515 ID=MS.gene022419.t1;Parent=MS.gene022419
chr3.2 exon 67975451 67975564 67975451 ID=MS.gene022419.t1.exon1;Parent=MS.gene022419.t1
chr3.2 CDS 67975451 67975564 67975451 ID=cds.MS.gene022419.t1;Parent=MS.gene022419.t1
chr3.2 exon 67975238 67975350 67975238 ID=MS.gene022419.t1.exon2;Parent=MS.gene022419.t1
chr3.2 CDS 67975238 67975350 67975238 ID=cds.MS.gene022419.t1;Parent=MS.gene022419.t1
chr3.2 exon 67974724 67975027 67974724 ID=MS.gene022419.t1.exon3;Parent=MS.gene022419.t1
chr3.2 CDS 67974724 67975027 67974724 ID=cds.MS.gene022419.t1;Parent=MS.gene022419.t1
chr3.2 exon 67974329 67974639 67974329 ID=MS.gene022419.t1.exon4;Parent=MS.gene022419.t1
chr3.2 CDS 67974329 67974639 67974329 ID=cds.MS.gene022419.t1;Parent=MS.gene022419.t1
chr3.2 exon 67973515 67973737 67973515 ID=MS.gene022419.t1.exon5;Parent=MS.gene022419.t1
chr3.2 CDS 67973515 67973737 67973515 ID=cds.MS.gene022419.t1;Parent=MS.gene022419.t1
Gene Sequence

>MS.gene022419

ATGGCTTCTCTGTTTTCTCCCATTGTCTCCCCCACCCGCTTTTCCCCCAAAACTTACATTTATATCCATACTCCCAGTGGCATAATACCCTTTCCAGTGCACGCAACCGTTCTGGTACTCTTCTATTTTCCTTCATGTCTCCGTTCATTTTCAATCACTATATTTTTTTATCGTCAATTAATTATGATTTTTCGTTCTAAAATTGATCTCAAAGGTTTCAGCGTCCCCAATTCTAAAAGAAATGGTAACGTCCAACGGTAACGTTAAAATATGTGGTGTTCCAACCAATGCAGCCATCGCCTTTGTACATTTCATCTACACTTCCAGGTCAGTAATAGTGAAATTAAATAACTACTCATTTTTACGTAGAAAAGAAAATTGAATTAACAAATTTTTTGGTCTAAATACATCAATTTTCGGTTACTCAATTTTCTGTCATATTTAGAACAAAGAGAGTAGTATTATACTTTTTTTCCCACTCATCCACTTGACAAAAAAAGACAATGGTGACCCTAAGAATTGATTGGGACTATGCAGGTGCAGCAGGGAGGATATGAAAAATTATGGAATACACCTTCTCGTACTCTCGCACGTCTTTGCAATGCCCAAACTAAAGCAAAGGTGCATTGTTGACTTGATCCAATTCATGACCACGGGGAATGTGGTGGATGTGTTGCACTTGGCTAAGCTTTGTGATGCACCAAATCTCTACTTCAAGTGTGTCAAGTTAGTTACCAATAATTTTGAAGCGGTGAAGGAAACAGAGGGGTGGAAATTATTGCATAAACATGACCCTTGGCTCGAAGTTGACATCATCCGTCTCAACAAAGAACGAGAATCGGTAAATAATAATCATTTTATTAAAATTCAGTAATTATATTTTATTTATATATTTCTTTAGTAAGTTGGTGATCGGGTGAAACAGAGGAAGAAGAGAGGAAATAAACATAGGGAGGAACAAAAGTTGTTTGTGCAATTAAGTGAAGCTGTACAATGTTTGGAGCATATATGTAGAGAAGGTTGCACCAACGTGGCATCATATGATGTGGAGATTACGGGGAGGCCGTGTACTAAATTCTCCACATGTCAAGCTTTGCAGGGGTTGATCAAACACTTTACCACATGTAATAGGAGACTCGAGAGAGGGTGTAGGTGTTGTAAGTCTATGTGGCAACTACTTAGACTGCACTCCTGCATTTGCTTTAACCAAGATGCATGCAAGGTTCCTCTTTGCAGGTATGCGAAACAATAATTAAAATTATGCATTTTTTCATTACATTTTTTCATATAAAAAAAGGAAAAACAAATAATAACTACAGCTGGTCTTTGTCATATAATTTTTTTTTATATTTTATTTATATGTTTTTTGTGTATTGTTATTAATCGTTATTATTTTGGATAAAGTATTTATATTAGTTTTTATTCTTTTTGTACAAAGTGTTGTTATTATTATTATTTTTGTTAAGTAGCAATTGACTAATTGAGGAATATAATGGTTTTTCATGTGATCAATTAAATTAAAGTGAATATGCAAACAAATTAACCTTTGAAATTAACCATCAATGGGTATATCATACAAAGAAATACATGTAGCTAACTACTAACGTGGCATATTTTTACATTCGTTGGGTGCACAAAGTGACAAAACCAACACGGTACGTATACATTATATGGTAATTGCATCTGTCTTCAATATCGTGAGCATGCATGCATGCCAAACATTTTAGTGAGTTAGTCATTGACTAATTATTTGTCTTAATTCTTCTAGTTACTAGTTTTCATTTAGGGTATTTGGTTAATAAAGCTATATTTACGGAAATGTGACAGGAAATATCAATTGATAGCTGAGAAAGAGAACAAGGAAGGTGATACGAGGTGGAAACAAATAGTGGGGAAAGTAGCATCAACCATAGCCATGTCTTCCTTACAATTGCTAAGGACAAGGAGAGATGAGGTAATTCGGAATACTAGAGTAGAGGAAGAGGAAGATCAGTCAAGAGAGTCTTCTAATTGGTGGACAAATGCAATTGCATGTTTTCGGTGTATCTAA
Protein sequence

>MS.gene022419.t1

MASLFSPIVSPTRFSPKTYIYIHTPSGIIPFPVHATVLVSASPILKEMVTSNGNVKICGVPTNAAIAFVHFIYTSRCSREDMKNYGIHLLVLSHVFAMPKLKQRCIVDLIQFMTTGNVVDVLHLAKLCDAPNLYFKCVKLVTNNFEAVKETEGWKLLHKHDPWLEVDIIRLNKERESRKKRGNKHREEQKLFVQLSEAVQCLEHICREGCTNVASYDVEITGRPCTKFSTCQALQGLIKHFTTCNRRLERGCRCCKSMWQLLRLHSCICFNQDACKVPLCRKYQLIAEKENKEGDTRWKQIVGKVASTIAMSSLQLLRTRRDEVIRNTRVEEEEDQSRESSNWWTNAIACFRCI