AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022567

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022567.t1 MTR_8g006705 98.366 306 4 1 1 306 1 305 0.0 620
MS.gene022567.t1 MTR_5g013010 58.387 310 96 11 3 304 4 288 2.42e-98 291
MS.gene022567.t1 MTR_4g100550 58.249 297 90 11 1 297 3 265 5.41e-95 282
MS.gene022567.t1 MTR_7g093430 64.706 187 51 4 78 260 98 273 3.23e-70 219
MS.gene022567.t1 MTR_1g054285 70.625 160 28 4 103 262 129 269 1.63e-67 213
MS.gene022567.t1 MTR_2g061030 74.648 142 31 2 129 270 187 323 7.90e-66 210
MS.gene022567.t1 MTR_4g097600 74.809 131 27 2 129 259 115 239 6.55e-64 202
MS.gene022567.t1 MTR_5g014890 76.667 120 27 1 140 259 117 235 4.49e-62 198
MS.gene022567.t1 MTR_1g017090 54.913 173 64 6 91 260 84 245 5.17e-53 175
MS.gene022567.t1 MTR_4g084750 63.710 124 40 1 140 258 59 182 8.85e-50 164
MS.gene022567.t1 MTR_5g013010 51.980 202 72 8 3 197 4 187 1.51e-45 153
MS.gene022567.t1 MTR_2g038625 57.031 128 48 1 132 259 75 195 6.63e-43 147
MS.gene022567.t1 MTR_2g038580 57.031 128 48 1 132 259 75 195 6.63e-43 147
MS.gene022567.t1 MTR_3g103590 64.516 124 42 2 145 268 144 265 8.07e-43 149
MS.gene022567.t1 MTR_4g126900 62.281 114 40 1 148 258 90 203 1.21e-37 133
MS.gene022567.t1 MTR_6g007647 45.631 103 54 1 143 243 30 132 2.90e-19 85.5
MS.gene022567.t1 MTR_6g007647 43.519 108 61 0 143 250 64 171 3.08e-19 86.3
MS.gene022567.t1 MTR_7g010020 47.222 108 48 2 132 235 71 173 3.63e-19 85.9
MS.gene022567.t1 MTR_5g039000 47.059 102 48 1 132 233 69 164 6.57e-19 85.1
MS.gene022567.t1 MTR_6g011610 40.164 122 73 0 112 233 62 183 2.01e-18 83.6
MS.gene022567.t1 MTR_6g011610 40.164 122 73 0 112 233 43 164 2.11e-18 83.2
MS.gene022567.t1 MTR_4g107650 45.690 116 61 1 120 233 67 182 3.12e-18 84.0
MS.gene022567.t1 MTR_8g468210 45.545 101 49 1 132 232 69 163 4.28e-18 82.8
MS.gene022567.t1 MTR_5g038280 48.515 101 44 2 146 239 87 186 1.07e-17 82.4
MS.gene022567.t1 MTR_1g061660 46.067 89 48 0 146 234 59 147 3.02e-17 79.7
MS.gene022567.t1 MTR_3g086790 40.152 132 68 4 136 257 49 179 9.33e-17 79.3
MS.gene022567.t1 MTR_7g103340 36.806 144 87 3 140 280 56 198 1.09e-16 78.2
MS.gene022567.t1 MTR_3g080100 38.519 135 78 2 146 276 20 153 2.23e-16 77.0
MS.gene022567.t1 MTR_3g080100 38.519 135 78 2 146 276 59 192 3.68e-16 77.0
MS.gene022567.t1 MTR_8g089895 37.838 148 79 4 133 273 43 184 3.71e-16 77.8
MS.gene022567.t1 MTR_8g469430 46.809 94 43 1 146 232 89 182 9.26e-16 76.3
MS.gene022567.t1 MTR_5g019650 42.593 108 59 2 135 240 43 149 1.65e-12 66.2
MS.gene022567.t1 MTR_5g019680 41.667 108 57 2 133 240 41 142 1.92e-12 65.5
MS.gene022567.t1 MTR_3g092150 41.667 96 43 2 149 234 41 133 2.37e-11 63.2
MS.gene022567.t1 MTR_8g026960 33.588 131 74 2 150 280 38 155 5.68e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022567.t1 AT3G60390 59.396 298 93 9 16 303 30 309 6.59e-102 301
MS.gene022567.t1 AT5G47370 53.674 313 87 14 1 301 3 269 5.25e-90 270
MS.gene022567.t1 AT2G44910 55.183 328 111 12 1 304 1 316 5.66e-88 266
MS.gene022567.t1 AT4G17460 52.769 307 98 11 1 299 3 270 4.27e-85 257
MS.gene022567.t1 AT4G16780 55.738 305 86 10 1 296 2 266 1.80e-83 253
MS.gene022567.t1 AT5G06710 66.138 189 49 5 90 271 134 314 6.53e-73 228
MS.gene022567.t1 AT4G37790 60.795 176 59 3 120 295 106 271 1.50e-65 207
MS.gene022567.t1 AT2G22800 75.833 120 28 1 140 259 107 225 3.70e-61 196
MS.gene022567.t1 AT2G01430 50.538 186 80 5 91 274 88 263 1.07e-49 166
MS.gene022567.t1 AT1G70920 56.028 141 48 2 130 270 59 185 3.93e-41 142
MS.gene022567.t1 AT1G70920 56.028 141 48 2 130 270 29 155 4.18e-41 141
MS.gene022567.t1 AT5G06710 59.649 114 32 4 91 197 135 241 6.11e-30 114
MS.gene022567.t1 AT2G01430 53.846 91 39 2 109 197 101 190 7.62e-22 91.7
MS.gene022567.t1 AT1G69780 37.079 178 88 3 70 233 5 172 7.68e-22 93.6
MS.gene022567.t1 AT1G26960 44.444 117 56 2 120 233 48 158 5.35e-19 85.1
MS.gene022567.t1 AT3G01470 48.387 93 41 1 146 231 68 160 1.48e-17 81.3
MS.gene022567.t1 AT5G65310 42.478 113 58 1 128 233 54 166 5.09e-17 80.5
MS.gene022567.t1 AT5G65310 42.478 113 58 1 128 233 36 148 5.68e-17 80.1
MS.gene022567.t1 AT5G15150 47.872 94 45 1 146 235 115 208 4.95e-16 77.4
MS.gene022567.t1 AT3G01220 45.745 94 47 1 146 235 87 180 2.20e-15 75.5
MS.gene022567.t1 AT5G03790 43.820 89 50 0 146 234 77 165 2.97e-15 74.3
MS.gene022567.t1 AT2G18550 43.137 102 53 1 140 241 55 151 8.60e-15 72.4
MS.gene022567.t1 AT4G40060 43.299 97 48 1 144 233 57 153 1.38e-14 73.2
MS.gene022567.t1 AT1G27045 45.833 96 45 1 146 234 68 163 2.72e-14 71.6
MS.gene022567.t1 AT1G27045 56.061 66 29 0 146 211 68 133 3.64e-14 70.9
MS.gene022567.t1 AT1G27045 56.061 66 29 0 146 211 25 90 5.11e-14 69.7
MS.gene022567.t1 AT1G27045 56.061 66 29 0 146 211 25 90 5.11e-14 69.7
MS.gene022567.t1 AT2G22430 45.833 96 48 1 144 235 60 155 5.34e-14 71.6
MS.gene022567.t1 AT1G27045 56.061 66 29 0 146 211 17 82 5.61e-14 69.3
MS.gene022567.t1 AT2G36610 36.937 111 58 2 136 234 57 167 2.17e-13 67.8
MS.gene022567.t1 AT2G46680 42.105 95 51 1 149 239 35 129 5.16e-13 68.2
MS.gene022567.t1 AT2G46680 42.045 88 51 0 149 236 35 122 6.74e-13 67.8
MS.gene022567.t1 AT4G36740 40.385 104 55 2 138 241 50 146 6.77e-13 67.0
MS.gene022567.t1 AT4G36740 40.385 104 55 2 138 241 49 145 7.00e-13 67.0
MS.gene022567.t1 AT5G03790 41.758 91 53 0 146 236 77 167 2.48e-12 65.1
MS.gene022567.t1 AT3G61890 33.333 126 84 0 149 274 33 158 1.69e-11 63.5
MS.gene022567.t1 AT5G06710 45.000 100 41 4 91 183 135 227 2.45e-11 63.2
MS.gene022567.t1 AT5G06710 45.000 100 41 4 91 183 135 227 2.45e-11 63.2

Find 91 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGGTCGGGAACGATTT+TGG 0.169525 8.4:+80340035 None:intergenic
GAAAGATGATGAATTTAGTT+TGG 0.275241 8.4:-80342336 MS.gene022567:CDS
CACTTCAGTTTGCTTCAACT+TGG 0.366945 8.4:+80340342 None:intergenic
TTAAGCTTGAGTTTAGGATT+TGG 0.367633 8.4:-80342311 MS.gene022567:CDS
AAAGATGATGAATTTAGTTT+GGG 0.373079 8.4:-80342335 MS.gene022567:CDS
CTTTGGTTTGTAACCGTTTG+AGG 0.393528 8.4:+80342230 None:intergenic
TGCGGCGGCCGTCGCCGCCA+TGG 0.395192 8.4:+80341648 None:intergenic
AAAAGGAAGTGCAAGAGCTT+AGG 0.411234 8.4:-80340261 MS.gene022567:CDS
TTGGGTTTAAGCTTGAGTTT+AGG 0.412484 8.4:-80342317 MS.gene022567:CDS
TCTGCTTCAGCCAACCAGTC+AGG 0.412655 8.4:-80340109 MS.gene022567:CDS
GGCCAACACCTGTCAACCCT+TGG 0.414534 8.4:-80340072 MS.gene022567:CDS
GAGTCACCTTCAAAGGAGTT+TGG 0.430355 8.4:+80342267 None:intergenic
AACCACACCTCCACTTGTCT+TGG 0.434860 8.4:+80341046 None:intergenic
AAAGGAAGTGCAAGAGCTTA+GGG 0.439484 8.4:-80340260 MS.gene022567:CDS
AGTTATTGAAGGAAACCCTT+TGG 0.441554 8.4:+80342213 None:intergenic
AACACTTGGGCCTGACTGGT+TGG 0.442499 8.4:+80340099 None:intergenic
GGTGTTGGCCGTTGAACACT+TGG 0.442745 8.4:+80340085 None:intergenic
GTTGAACACTTGGGCCTGAC+TGG 0.443989 8.4:+80340095 None:intergenic
CAACAACCAAACTCCTTTGA+AGG 0.444372 8.4:-80342273 MS.gene022567:CDS
GTTGTTGGAAGAAACTTTCA+AGG 0.454317 8.4:-80341410 MS.gene022567:CDS
ATGCTTGACACGGTGCTGTT+CGG 0.456039 8.4:+80341598 None:intergenic
AGCAGCAGCAGCTGCTGATG+AGG 0.465286 8.4:+80340138 None:intergenic
GCGGCCGTCGCCGCCATGGA+TGG 0.472789 8.4:+80341652 None:intergenic
GCCCAAGGGTTGACAGGTGT+TGG 0.475640 8.4:+80340070 None:intergenic
GCCAACACCTGTCAACCCTT+GGG 0.479663 8.4:-80340071 MS.gene022567:CDS
GATGATGACGGCGGAGGTTG+CGG 0.480696 8.4:-80341487 MS.gene022567:CDS
AGACTCTAACTGATGAGAAT+AGG 0.482124 8.4:-80340291 MS.gene022567:CDS
GGTTCATGTTCTCGCGGCGG+CGG 0.496150 8.4:-80341517 MS.gene022567:CDS
GAAGGAACAGTCAATGTTGT+TGG 0.498061 8.4:-80341425 MS.gene022567:CDS
ATTGCCGCCCAAGGGTTGAC+AGG 0.503094 8.4:+80340064 None:intergenic
TTCATTTGCATGTAGAGTTG+TGG 0.503551 8.4:+80340223 None:intergenic
CGGCGACACGCTCGCACGAA+GGG 0.511247 8.4:+80340176 None:intergenic
TCATTTCTCCGCGGAATAGA+CGG 0.512734 8.4:-80341688 MS.gene022567:CDS
GGCGGAGGTTGCGGTGGTGA+CGG 0.513641 8.4:-80341478 MS.gene022567:CDS
TGAAAACGACGCCGTCGAAA+GGG 0.514530 8.4:-80341539 MS.gene022567:CDS
TGGATAGACATTGCCGCCCA+AGG 0.515878 8.4:+80340055 None:intergenic
CTTTCCACTGATGCTTGACA+CGG 0.517994 8.4:+80341588 None:intergenic
AAAATGGAACAAGTTATTGA+AGG 0.520682 8.4:+80342202 None:intergenic
AGAAGCGAAAGAGAAGGAAA+CGG 0.525139 8.4:-80341565 MS.gene022567:CDS
CATGCACAAGCCTCCTCAAA+CGG 0.530251 8.4:-80342243 MS.gene022567:CDS
GGAGCATAATACTCTGAATC+CGG 0.531921 8.4:-80341389 MS.gene022567:intron
GCCGCATTCGACGATGAAAA+CGG 0.539309 8.4:-80341631 MS.gene022567:CDS
TGCATGTGAGTCACCTTCAA+AGG 0.543980 8.4:+80342260 None:intergenic
ACTACCATCCATGGCGGCGA+CGG 0.547385 8.4:-80341656 MS.gene022567:CDS
TGACTGGTTGGCTGAAGCAG+AGG 0.549119 8.4:+80340111 None:intergenic
CAACACTCCACTACCATCCA+TGG 0.551656 8.4:-80341665 MS.gene022567:CDS
TAGAGCGAATACTCATGGTC+GGG 0.553208 8.4:+80340024 None:intergenic
CTCTAACTGATGAGAATAGG+AGG 0.555223 8.4:-80340288 MS.gene022567:CDS
GACTGGTTGGCTGAAGCAGA+GGG 0.557071 8.4:+80340112 None:intergenic
TCAGAAACAAAAGCAAGCAT+TGG 0.557840 8.4:-80341089 MS.gene022567:intron
GTCGCCGCCATGGATGGTAG+TGG 0.558543 8.4:+80341658 None:intergenic
GGAAAGAGAAGCGAAAGAGA+AGG 0.564040 8.4:-80341571 MS.gene022567:CDS
TGAGAATAGGAGGTTGCAAA+AGG 0.565911 8.4:-80340278 MS.gene022567:CDS
CTCAAACGGTTACAAACCAA+AGG 0.569424 8.4:-80342229 MS.gene022567:CDS
ATGAAAACGACGCCGTCGAA+AGG 0.571630 8.4:-80341540 MS.gene022567:CDS
GGATAGACATTGCCGCCCAA+GGG 0.571951 8.4:+80340056 None:intergenic
GGGCACATTGTAAGAGTGGT+GGG 0.573198 8.4:+80340196 None:intergenic
CAAGTTGAAGCAAACTGAAG+TGG 0.573772 8.4:-80340341 MS.gene022567:CDS
CTAGAGCGAATACTCATGGT+CGG 0.575049 8.4:+80340023 None:intergenic
CGAAGGGCACATTGTAAGAG+TGG 0.588926 8.4:+80340192 None:intergenic
AACACCTGTCAACCCTTGGG+CGG 0.591322 8.4:-80340068 MS.gene022567:CDS
TCAAACGGTTACAAACCAAA+GGG 0.592760 8.4:-80342228 MS.gene022567:CDS
ACATTGTAAGAGTGGTGGGA+GGG 0.595529 8.4:+80340200 None:intergenic
GTGTTGGCCGTTGAACACTT+GGG 0.596735 8.4:+80340086 None:intergenic
ACGGCGACACGCTCGCACGA+AGG 0.599222 8.4:+80340175 None:intergenic
AGCCAAGACAAGTGGAGGTG+TGG 0.599876 8.4:-80341048 MS.gene022567:CDS
TGGTTTGTAACCGTTTGAGG+AGG 0.599884 8.4:+80342233 None:intergenic
AGCACCGTGTCAAGCATCAG+TGG 0.602338 8.4:-80341592 MS.gene022567:CDS
AGGGCACATTGTAAGAGTGG+TGG 0.603305 8.4:+80340195 None:intergenic
AGTCAGGCCCAAGTGTTCAA+CGG 0.603538 8.4:-80340093 MS.gene022567:CDS
AGAACATGAACCCCTTTCGA+CGG 0.611067 8.4:+80341528 None:intergenic
CACATTGTAAGAGTGGTGGG+AGG 0.613092 8.4:+80340199 None:intergenic
GAAAACGACGCCGTCGAAAG+GGG 0.619048 8.4:-80341538 MS.gene022567:CDS
GAATCTGAAGCCAAGACAAG+TGG 0.625066 8.4:-80341056 MS.gene022567:CDS
GAAGAAGCTGAGGTTGTCGA+AGG 0.629748 8.4:-80341443 MS.gene022567:CDS
GATGACGGCGGAGGTTGCGG+TGG 0.634690 8.4:-80341484 MS.gene022567:CDS
AGTGATGATGATGACGGCGG+AGG 0.634764 8.4:-80341493 MS.gene022567:CDS
TCTGAAGCCAAGACAAGTGG+AGG 0.635230 8.4:-80341053 MS.gene022567:CDS
GTGGTGACGGTGATTCGTCA+AGG 0.643931 8.4:-80341465 MS.gene022567:CDS
ATTCGTCAAGGAAGAAGCTG+AGG 0.662832 8.4:-80341453 MS.gene022567:CDS
GAAAGGGGTTCATGTTCTCG+CGG 0.668678 8.4:-80341523 MS.gene022567:CDS
GGCGGAAGTGATGATGATGA+CGG 0.668902 8.4:-80341499 MS.gene022567:CDS
GGTTTCAAAACAGAAGAGCA+AGG 0.676969 8.4:-80341027 MS.gene022567:intron
CAGATCTAAGCACAGAAACG+AGG 0.678976 8.4:-80341711 MS.gene022567:CDS
GAGTGTTGCCGTCTATTCCG+CGG 0.683806 8.4:+80341680 None:intergenic
TGGTTGGCTGAAGCAGAGGG+AGG 0.685549 8.4:+80340115 None:intergenic
TGAGGACGATGCAGATGACA+CGG 0.688825 8.4:+80340156 None:intergenic
AGGGGTTCATGTTCTCGCGG+CGG 0.699189 8.4:-80341520 MS.gene022567:CDS
CACTCCACTACCATCCATGG+CGG 0.704044 8.4:-80341662 MS.gene022567:CDS
GGAAGTGATGATGATGACGG+CGG 0.716365 8.4:-80341496 MS.gene022567:CDS
GAAACGAGGTCATTTCTCCG+CGG 0.771153 8.4:-80341697 MS.gene022567:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAAAATAAAAAAAAAA+TGG + chr8.4:80340422-80340441 None:intergenic 0.0%
!! AAAAAAAAAAATAAGGAGAA+AGG + chr8.4:80340242-80340261 None:intergenic 15.0%
!! GTAATTATCAACTAAATTTA+AGG + chr8.4:80340611-80340630 None:intergenic 15.0%
!! TCAGAACAAAAAAAAAAATA+AGG + chr8.4:80340249-80340268 None:intergenic 15.0%
!!! ATTTCTCTTCATATTTTTTA+GGG - chr8.4:80340284-80340303 MS.gene022567:CDS 15.0%
!!! GTCATTTTTGAATATTAATT+TGG - chr8.4:80341405-80341424 MS.gene022567:CDS 15.0%
!!! TCATTTTTGGTTTTTTTTTT+GGG - chr8.4:80340328-80340347 MS.gene022567:CDS 15.0%
!!! TTATTATAATCTTTTGATGA+TGG - chr8.4:80341780-80341799 MS.gene022567:intron 15.0%
!!! TTGTTTTTATTATTGTTTTG+AGG - chr8.4:80341136-80341155 MS.gene022567:intron 15.0%
!!! TTTTTCATTATATGATTCAA+GGG + chr8.4:80340395-80340414 None:intergenic 15.0%
!! AAAAAACCAAAAATGAGAAA+TGG + chr8.4:80340324-80340343 None:intergenic 20.0%
!! AAAAACCAAAAATGAGAAAT+GGG + chr8.4:80340323-80340342 None:intergenic 20.0%
!! TGTAAACTCTTAAAATTGAA+AGG + chr8.4:80341828-80341847 None:intergenic 20.0%
!! TTTATGAGGAGTAAAAAATT+AGG - chr8.4:80341655-80341674 MS.gene022567:CDS 20.0%
!!! AAAGATGATGAATTTAGTTT+GGG - chr8.4:80340045-80340064 MS.gene022567:CDS 20.0%
!!! CTCATTTTTGGTTTTTTTTT+TGG - chr8.4:80340327-80340346 MS.gene022567:CDS 20.0%
!!! GATTTCTCTTCATATTTTTT+AGG - chr8.4:80340283-80340302 MS.gene022567:CDS 20.0%
!!! GTAATTCAGTTTTATGTTAA+GGG - chr8.4:80341038-80341057 MS.gene022567:CDS 20.0%
!!! GTTTTTCATTATATGATTCA+AGG + chr8.4:80340396-80340415 None:intergenic 20.0%
!!! TATATTTGGAAGTGTAAATT+TGG - chr8.4:80341093-80341112 MS.gene022567:intron 20.0%
!!! TGTAATTCAGTTTTATGTTA+AGG - chr8.4:80341037-80341056 MS.gene022567:CDS 20.0%
!!! TTTTTTGGTTGAGTAATTTA+AGG - chr8.4:80341179-80341198 MS.gene022567:intron 20.0%
! AAAACCAAAAATGAGAAATG+GGG + chr8.4:80340322-80340341 None:intergenic 25.0%
! AAAATGGAACAAGTTATTGA+AGG + chr8.4:80340181-80340200 None:intergenic 25.0%
! ACTGTTTGGATCATATTAAT+TGG - chr8.4:80341526-80341545 MS.gene022567:CDS 25.0%
! ATATGAAGAGAAATCAAGAA+TGG + chr8.4:80340278-80340297 None:intergenic 25.0%
! ATTCATCTCATCAAAGAAAA+TGG + chr8.4:80340498-80340517 None:intergenic 25.0%
! GAAAAAACATACCATGAAAA+TGG + chr8.4:80340197-80340216 None:intergenic 25.0%
! GAAAGATGATGAATTTAGTT+TGG - chr8.4:80340044-80340063 MS.gene022567:CDS 25.0%
! GAAAGGAAACATAGAAATTT+AGG + chr8.4:80340225-80340244 None:intergenic 25.0%
! GAATTTCATTACAGGATAAA+AGG - chr8.4:80341069-80341088 MS.gene022567:CDS 25.0%
! TATGAAGAGAAATCAAGAAT+GGG + chr8.4:80340277-80340296 None:intergenic 25.0%
! TTCATCTCATCAAAGAAAAT+GGG + chr8.4:80340497-80340516 None:intergenic 25.0%
!! AACACAACTTAACTTTTATG+AGG - chr8.4:80341641-80341660 MS.gene022567:CDS 25.0%
!! CTTATTTGGAATTTCATTAC+AGG - chr8.4:80341061-80341080 MS.gene022567:CDS 25.0%
!! TTTTATGTTAAGGGCTTATT+TGG - chr8.4:80341047-80341066 MS.gene022567:CDS 25.0%
!!! AATAACTTGTTCCATTTTCA+TGG - chr8.4:80340183-80340202 MS.gene022567:CDS 25.0%
!!! ATAATCTTTTGATGATGGAT+TGG - chr8.4:80341785-80341804 MS.gene022567:intron 25.0%
!!! TAATCTTTTGATGATGGATT+GGG - chr8.4:80341786-80341805 MS.gene022567:intron 25.0%
ACAGGATAAAAGGCTATATT+TGG - chr8.4:80341079-80341098 MS.gene022567:CDS 30.0%
GGTTATACCAAAAATGAGAA+GGG + chr8.4:80340374-80340393 None:intergenic 30.0%
GTTATACCAAAAATGAGAAG+GGG + chr8.4:80340373-80340392 None:intergenic 30.0%
GTTGTATTGTAACAAGTATG+TGG - chr8.4:80341695-80341714 MS.gene022567:CDS 30.0%
TAAACATTGTTGTGATGTTG+TGG - chr8.4:80341439-80341458 MS.gene022567:CDS 30.0%
TAATATGATCCAAACAGTGT+GGG + chr8.4:80341524-80341543 None:intergenic 30.0%
TTAATATGATCCAAACAGTG+TGG + chr8.4:80341525-80341544 None:intergenic 30.0%
! TACATTTTTGTGCATGTAGT+AGG + chr8.4:80341751-80341770 None:intergenic 30.0%
! TTAAGCTTGAGTTTAGGATT+TGG - chr8.4:80340069-80340088 MS.gene022567:CDS 30.0%
!!! ATTGTTTTGAGGTTATTCTG+AGG - chr8.4:80341147-80341166 MS.gene022567:intron 30.0%
!!! CTGAGGTTGTCTTTTTTTTT+TGG - chr8.4:80341164-80341183 MS.gene022567:intron 30.0%
!!! TGGTTTTTTTTTTGGGTGAA+TGG - chr8.4:80340335-80340354 MS.gene022567:CDS 30.0%
!!! TTTTTTTGGGTGAATGGTAA+GGG - chr8.4:80340341-80340360 MS.gene022567:CDS 30.0%
!!! TTTTTTTTGGGTGAATGGTA+AGG - chr8.4:80340340-80340359 MS.gene022567:CDS 30.0%
AAAATGTGACATGAGTGCTT+TGG - chr8.4:80342000-80342019 MS.gene022567:intron 35.0%
AATATGATCCAAACAGTGTG+GGG + chr8.4:80341523-80341542 None:intergenic 35.0%
ACATGAGTATGTTAGGACTT+AGG - chr8.4:80341720-80341739 MS.gene022567:intron 35.0%
AGACTCTAACTGATGAGAAT+AGG - chr8.4:80342089-80342108 MS.gene022567:intron 35.0%
AGTTATTGAAGGAAACCCTT+TGG + chr8.4:80340170-80340189 None:intergenic 35.0%
CACAACAAATCCACATACAT+TGG + chr8.4:80341920-80341939 None:intergenic 35.0%
CATGAGTATGTTAGGACTTA+GGG - chr8.4:80341721-80341740 MS.gene022567:intron 35.0%
CATGTACAAACCAATGTATG+TGG - chr8.4:80341907-80341926 MS.gene022567:intron 35.0%
GGGTTATACCAAAAATGAGA+AGG + chr8.4:80340375-80340394 None:intergenic 35.0%
GTTGTTGGAAGAAACTTTCA+AGG - chr8.4:80340970-80340989 MS.gene022567:intron 35.0%
TATTGTGTTCCATGCTGTTA+TGG - chr8.4:80341967-80341986 MS.gene022567:intron 35.0%
TCAAACGGTTACAAACCAAA+GGG - chr8.4:80340152-80340171 MS.gene022567:CDS 35.0%
TCAGAAACAAAAGCAAGCAT+TGG - chr8.4:80341291-80341310 MS.gene022567:intron 35.0%
TTCATTTGCATGTAGAGTTG+TGG + chr8.4:80342160-80342179 None:intergenic 35.0%
TTGGGTTTAAGCTTGAGTTT+AGG - chr8.4:80340063-80340082 MS.gene022567:CDS 35.0%
! AGAACCCCATTTCTCATTTT+TGG - chr8.4:80340315-80340334 MS.gene022567:CDS 35.0%
! ATTTTTGTGCATGTAGTAGG+TGG + chr8.4:80341748-80341767 None:intergenic 35.0%
! TTTTTCCTTCCATAACAGCA+TGG + chr8.4:80341979-80341998 None:intergenic 35.0%
! TTTTTGTGCATGTAGTAGGT+GGG + chr8.4:80341747-80341766 None:intergenic 35.0%
!! TTTGCATGTGAGTGTTTTAG+TGG + chr8.4:80340641-80340660 None:intergenic 35.0%
!!! TTTTTTGGGTGAATGGTAAG+GGG - chr8.4:80340342-80340361 MS.gene022567:CDS 35.0%
ACAGTGTGGGGTATATAGTA+TGG + chr8.4:80341511-80341530 None:intergenic 40.0%
ACTATATACCCCACACTGTT+TGG - chr8.4:80341512-80341531 MS.gene022567:CDS 40.0%
AGAAGCGAAAGAGAAGGAAA+CGG - chr8.4:80340815-80340834 MS.gene022567:intron 40.0%
CAACAACCAAACTCCTTTGA+AGG - chr8.4:80340107-80340126 MS.gene022567:CDS 40.0%
CTCAAACGGTTACAAACCAA+AGG - chr8.4:80340151-80340170 MS.gene022567:CDS 40.0%
CTCTAACTGATGAGAATAGG+AGG - chr8.4:80342092-80342111 MS.gene022567:intron 40.0%
GAAGGAACAGTCAATGTTGT+TGG - chr8.4:80340955-80340974 MS.gene022567:intron 40.0%
GGAGCATAATACTCTGAATC+CGG - chr8.4:80340991-80341010 MS.gene022567:intron 40.0%
GGTTTCAAAACAGAAGAGCA+AGG - chr8.4:80341353-80341372 MS.gene022567:intron 40.0%
TACCACTACAACAAACTCAC+CGG + chr8.4:80341013-80341032 None:intergenic 40.0%
TGAGAATAGGAGGTTGCAAA+AGG - chr8.4:80342102-80342121 MS.gene022567:intron 40.0%
TGTGGCAACATGAGTATGTT+AGG - chr8.4:80341713-80341732 MS.gene022567:intron 40.0%
! AAAAGGAAGTGCAAGAGCTT+AGG - chr8.4:80342119-80342138 MS.gene022567:intron 40.0%
! AAAGGAAGTGCAAGAGCTTA+GGG - chr8.4:80342120-80342139 MS.gene022567:intron 40.0%
! CAAGTTGAAGCAAACTGAAG+TGG - chr8.4:80342039-80342058 MS.gene022567:intron 40.0%
! CACTTCAGTTTGCTTCAACT+TGG + chr8.4:80342041-80342060 None:intergenic 40.0%
! GAAAAGCCCCTTCTCATTTT+TGG - chr8.4:80340364-80340383 MS.gene022567:intron 40.0%
!! CTTTGGTTTGTAACCGTTTG+AGG + chr8.4:80340153-80340172 None:intergenic 40.0%
!!! CTTTTGATGATGGATTGGGA+AGG - chr8.4:80341790-80341809 MS.gene022567:intron 40.0%
AGAACATGAACCCCTTTCGA+CGG + chr8.4:80340855-80340874 None:intergenic 45.0%
AGGTGAGCTTTCTTCTTCTC+AGG - chr8.4:80341373-80341392 MS.gene022567:intron 45.0%
ATTCGTCAAGGAAGAAGCTG+AGG - chr8.4:80340927-80340946 MS.gene022567:intron 45.0%
CAGATCTAAGCACAGAAACG+AGG - chr8.4:80340669-80340688 MS.gene022567:intron 45.0%
CTTTCCACTGATGCTTGACA+CGG + chr8.4:80340795-80340814 None:intergenic 45.0%
GAGTCACCTTCAAAGGAGTT+TGG + chr8.4:80340116-80340135 None:intergenic 45.0%
GGAAAGAGAAGCGAAAGAGA+AGG - chr8.4:80340809-80340828 MS.gene022567:intron 45.0%
GGTGAGCTTTCTTCTTCTCA+GGG - chr8.4:80341374-80341393 MS.gene022567:intron 45.0%
GTGAGCTTTCTTCTTCTCAG+GGG - chr8.4:80341375-80341394 MS.gene022567:intron 45.0%
GTGTTCCATGCTGTTATGGA+AGG - chr8.4:80341971-80341990 MS.gene022567:intron 45.0%
TCATTTCTCCGCGGAATAGA+CGG - chr8.4:80340692-80340711 MS.gene022567:intron 45.0%
TGCATGTGAGTCACCTTCAA+AGG + chr8.4:80340123-80340142 None:intergenic 45.0%
! ACATTGTAAGAGTGGTGGGA+GGG + chr8.4:80342183-80342202 None:intergenic 45.0%
! GAATCTGAAGCCAAGACAAG+TGG - chr8.4:80341324-80341343 MS.gene022567:intron 45.0%
! GTGCTTTGGTTCAATGATGC+AGG - chr8.4:80342014-80342033 MS.gene022567:intron 45.0%
! TCCGTTTTCATCGTCGAATG+CGG + chr8.4:80340753-80340772 None:intergenic 45.0%
! TGGTTTGTAACCGTTTGAGG+AGG + chr8.4:80340150-80340169 None:intergenic 45.0%
!! ATCCGGTGAGTTTGTTGTAG+TGG - chr8.4:80341008-80341027 MS.gene022567:intron 45.0%
!! TATTTATTTATGAATTAAAA+CGG - chr8.4:80340566-80340585 MS.gene022567:intron 5.0%
AACCACACCTCCACTTGTCT+TGG + chr8.4:80341337-80341356 None:intergenic 50.0%
AGGGCACATTGTAAGAGTGG+TGG + chr8.4:80342188-80342207 None:intergenic 50.0%
AGTCAGGCCCAAGTGTTCAA+CGG - chr8.4:80342287-80342306 MS.gene022567:CDS 50.0%
ATGAAAACGACGCCGTCGAA+AGG - chr8.4:80340840-80340859 MS.gene022567:intron 50.0%
CAACACTCCACTACCATCCA+TGG - chr8.4:80340715-80340734 MS.gene022567:intron 50.0%
CATGCACAAGCCTCCTCAAA+CGG - chr8.4:80340137-80340156 MS.gene022567:CDS 50.0%
GAAACGAGGTCATTTCTCCG+CGG - chr8.4:80340683-80340702 MS.gene022567:intron 50.0%
GAAAGGGGTTCATGTTCTCG+CGG - chr8.4:80340857-80340876 MS.gene022567:intron 50.0%
GAAGAAGCTGAGGTTGTCGA+AGG - chr8.4:80340937-80340956 MS.gene022567:intron 50.0%
GCCGCATTCGACGATGAAAA+CGG - chr8.4:80340749-80340768 MS.gene022567:intron 50.0%
GGGCACATTGTAAGAGTGGT+GGG + chr8.4:80342187-80342206 None:intergenic 50.0%
TGAAAACGACGCCGTCGAAA+GGG - chr8.4:80340841-80340860 MS.gene022567:intron 50.0%
TGAGGACGATGCAGATGACA+CGG + chr8.4:80342227-80342246 None:intergenic 50.0%
! CACATTGTAAGAGTGGTGGG+AGG + chr8.4:80342184-80342203 None:intergenic 50.0%
! CGAAGGGCACATTGTAAGAG+TGG + chr8.4:80342191-80342210 None:intergenic 50.0%
! GGCGGAAGTGATGATGATGA+CGG - chr8.4:80340881-80340900 MS.gene022567:intron 50.0%
! GTGTTGGCCGTTGAACACTT+GGG + chr8.4:80342297-80342316 None:intergenic 50.0%
! GTTTTCATCGTCGAATGCGG+CGG + chr8.4:80340750-80340769 None:intergenic 50.0%
! TCTGAAGCCAAGACAAGTGG+AGG - chr8.4:80341327-80341346 MS.gene022567:intron 50.0%
!! ATGCTTGACACGGTGCTGTT+CGG + chr8.4:80340785-80340804 None:intergenic 50.0%
!! GGAAGTGATGATGATGACGG+CGG - chr8.4:80340884-80340903 MS.gene022567:intron 50.0%
AACACCTGTCAACCCTTGGG+CGG - chr8.4:80342312-80342331 MS.gene022567:CDS 55.0%
AACACTTGGGCCTGACTGGT+TGG + chr8.4:80342284-80342303 None:intergenic 55.0%
AGCACCGTGTCAAGCATCAG+TGG - chr8.4:80340788-80340807 MS.gene022567:intron 55.0%
AGCCAAGACAAGTGGAGGTG+TGG - chr8.4:80341332-80341351 MS.gene022567:intron 55.0%
CACTCCACTACCATCCATGG+CGG - chr8.4:80340718-80340737 MS.gene022567:intron 55.0%
GAAAACGACGCCGTCGAAAG+GGG - chr8.4:80340842-80340861 MS.gene022567:intron 55.0%
GCCAACACCTGTCAACCCTT+GGG - chr8.4:80342309-80342328 MS.gene022567:CDS 55.0%
GGATAGACATTGCCGCCCAA+GGG + chr8.4:80342327-80342346 None:intergenic 55.0%
GTTGAACACTTGGGCCTGAC+TGG + chr8.4:80342288-80342307 None:intergenic 55.0%
TCTGCTTCAGCCAACCAGTC+AGG - chr8.4:80342271-80342290 MS.gene022567:CDS 55.0%
TGGATAGACATTGCCGCCCA+AGG + chr8.4:80342328-80342347 None:intergenic 55.0%
! GACTGGTTGGCTGAAGCAGA+GGG + chr8.4:80342271-80342290 None:intergenic 55.0%
! GAGTGTTGCCGTCTATTCCG+CGG + chr8.4:80340703-80340722 None:intergenic 55.0%
! TGACTGGTTGGCTGAAGCAG+AGG + chr8.4:80342272-80342291 None:intergenic 55.0%
!! AGTGATGATGATGACGGCGG+AGG - chr8.4:80340887-80340906 MS.gene022567:intron 55.0%
!! GGTGTTGGCCGTTGAACACT+TGG + chr8.4:80342298-80342317 None:intergenic 55.0%
!! GTGGTGACGGTGATTCGTCA+AGG - chr8.4:80340915-80340934 MS.gene022567:intron 55.0%
ACTACCATCCATGGCGGCGA+CGG - chr8.4:80340724-80340743 MS.gene022567:intron 60.0%
AGCAGCAGCAGCTGCTGATG+AGG + chr8.4:80342245-80342264 None:intergenic 60.0%
AGGGGTTCATGTTCTCGCGG+CGG - chr8.4:80340860-80340879 MS.gene022567:intron 60.0%
ATTGCCGCCCAAGGGTTGAC+AGG + chr8.4:80342319-80342338 None:intergenic 60.0%
GCCCAAGGGTTGACAGGTGT+TGG + chr8.4:80342313-80342332 None:intergenic 60.0%
GGCCAACACCTGTCAACCCT+TGG - chr8.4:80342308-80342327 MS.gene022567:CDS 60.0%
! TGGTTGGCTGAAGCAGAGGG+AGG + chr8.4:80342268-80342287 None:intergenic 60.0%
!! GATGATGACGGCGGAGGTTG+CGG - chr8.4:80340893-80340912 MS.gene022567:intron 60.0%
GGTTCATGTTCTCGCGGCGG+CGG - chr8.4:80340863-80340882 MS.gene022567:intron 65.0%
GTCGCCGCCATGGATGGTAG+TGG + chr8.4:80340725-80340744 None:intergenic 65.0%
ACGGCGACACGCTCGCACGA+AGG + chr8.4:80342208-80342227 None:intergenic 70.0%
CGGCGACACGCTCGCACGAA+GGG + chr8.4:80342207-80342226 None:intergenic 70.0%
GGCGGAGGTTGCGGTGGTGA+CGG - chr8.4:80340902-80340921 MS.gene022567:intron 70.0%
! GATGACGGCGGAGGTTGCGG+TGG - chr8.4:80340896-80340915 MS.gene022567:intron 70.0%
! GCGGCCGTCGCCGCCATGGA+TGG + chr8.4:80340731-80340750 None:intergenic 80.0%
! TGCGGCGGCCGTCGCCGCCA+TGG + chr8.4:80340735-80340754 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 80340036 80342366 80340036 ID=MS.gene022567
chr8.4 mRNA 80340036 80342366 80340036 ID=MS.gene022567.t1;Parent=MS.gene022567
chr8.4 exon 80342198 80342366 80342198 ID=MS.gene022567.t1.exon1;Parent=MS.gene022567.t1
chr8.4 CDS 80342198 80342366 80342198 ID=cds.MS.gene022567.t1;Parent=MS.gene022567.t1
chr8.4 exon 80341390 80341730 80341390 ID=MS.gene022567.t1.exon2;Parent=MS.gene022567.t1
chr8.4 CDS 80341390 80341730 80341390 ID=cds.MS.gene022567.t1;Parent=MS.gene022567.t1
chr8.4 exon 80341028 80341107 80341028 ID=MS.gene022567.t1.exon3;Parent=MS.gene022567.t1
chr8.4 CDS 80341028 80341107 80341028 ID=cds.MS.gene022567.t1;Parent=MS.gene022567.t1
chr8.4 exon 80340036 80340366 80340036 ID=MS.gene022567.t1.exon4;Parent=MS.gene022567.t1
chr8.4 CDS 80340036 80340366 80340036 ID=cds.MS.gene022567.t1;Parent=MS.gene022567.t1
Gene Sequence

>MS.gene022567

ATGGGTGAGAAAGATGATGAATTTAGTTTGGGTTTAAGCTTGAGTTTAGGATTTGGAGAATATGCAAATAACAACAACCAAACTCCTTTGAAGGTGACTCACATGCACAAGCCTCCTCAAACGGTTACAAACCAAAGGGTTTCCTTCAATAACTTGTTCCATTTTCATGGTATGTTTTTTCTCTCTCCTAAATTTCTATGTTTCCTTTCTCCTTATTTTTTTTTTTGTTCTGAAAAAACCCATTCTTGATTTCTCTTCATATTTTTTAGGGTAAATACTAGAACCCCATTTCTCATTTTTGGTTTTTTTTTTGGGTGAATGGTAAGGGGAAAAGCCCCTTCTCATTTTTGGTATAACCCTTGAATCATATAATGAAAAACATACCATTTTTTTTTTATTTTTTATAAAAAAAACTATTTTTTACACTAAAAATAGATTTTTTTAATCATAACTAAAAACCCATTTTCTTTGATGAGATGAATTCAAGTCATGAAAATGAAGAACACATTTTAATTTACTTTCTGATTTTTTATTTATTTATGAATTAAAACGGTTTTGTTACAAAAAAATAGCCTTAAATTTAGTTGATAATTACACGTTTACCACTAAAACACTCACATGCAAATCACGTGACAGATCTAAGCACAGAAACGAGGTCATTTCTCCGCGGAATAGACGGCAACACTCCACTACCATCCATGGCGGCGACGGCCGCCGCATTCGACGATGAAAACGGAGTTTCATCACCGAACAGCACCGTGTCAAGCATCAGTGGAAAGAGAAGCGAAAGAGAAGGAAACGGAGATGAAAACGACGCCGTCGAAAGGGGTTCATGTTCTCGCGGCGGCGGAAGTGATGATGATGACGGCGGAGGTTGCGGTGGTGACGGTGATTCGTCAAGGAAGAAGCTGAGGTTGTCGAAGGAACAGTCAATGTTGTTGGAAGAAACTTTCAAGGAGCATAATACTCTGAATCCGGTGAGTTTGTTGTAGTGGTATTTCTGTAATTCAGTTTTATGTTAAGGGCTTATTTGGAATTTCATTACAGGATAAAAGGCTATATTTGGAAGTGTAAATTTGGTTGTTTTGACTTGTTCTTTTTTGTTTTTATTATTGTTTTGAGGTTATTCTGAGGTTGTCTTTTTTTTTTGGTTGAGTAATTTAAGGTTGTCTTTCTTTATATAATCTTAAAATAATTATTTTAATTATTACTTTGATTTTGATTTTAATTATTATTATTATTATTGTTATTATTATCAGAAACAAAAGCAAGCATTGGCAAAGCAGTTGAATCTGAAGCCAAGACAAGTGGAGGTGTGGTTTCAAAACAGAAGAGCAAGGTGAGCTTTCTTCTTCTCAGGGGCATTACTGTCATTTTTGAATATTAATTTGGAATTAGTAAAATAAACATTGTTGTGATGTTGTGGCATTGTTTGTTGTGTTATGTGTGACTTTGTAAGCTTCTGTTGCATACCATACTATATACCCCACACTGTTTGGATCATATTAATTGGTTTTGTTTTTTACTAATTAAAATGTTAGCTATCACTAAAAAAAAATTAAAATGTTAATATTACTCTTTGAAATAAATCTATGAGTGATCAATAACACAACTTAACTTTTATGAGGAGTAAAAAATTAGGCATAAAAAAAATTTGAAGTTGTATTGTAACAAGTATGTGGCAACATGAGTATGTTAGGACTTAGGGCCCACCTACTACATGCACAAAAATGTAACAAGCATGTTATTATAATCTTTTGATGATGGATTGGGAAGGCAAAATTTGAAACCTTTCAATTTTAAGAGTTTACATACATTCATTAATTTCTCATGAGTTAAATTAGTTAATAGAAGAAATACTTTTAACATGTCATGTACAAACCAATGTATGTGGATTTGTTGTGTTATGTGTATGTGTTTCATGTGTGTTGTATTGTGTTCCATGCTGTTATGGAAGGAAAAAAAAAATGTGACATGAGTGCTTTGGTTCAATGATGCAGGACCAAGTTGAAGCAAACTGAAGTGGATTGTGAGTACTTGAAGAGATGCTGTGAGACTCTAACTGATGAGAATAGGAGGTTGCAAAAGGAAGTGCAAGAGCTTAGGGCATTGAAGTTATCTCCACAACTCTACATGCAAATGAACCCTCCCACCACTCTTACAATGTGCCCTTCGTGCGAGCGTGTCGCCGTGTCATCTGCATCGTCCTCATCAGCAGCTGCTGCTGCTCCTCCCTCTGCTTCAGCCAACCAGTCAGGCCCAAGTGTTCAACGGCCAACACCTGTCAACCCTTGGGCGGCAATGTCTATCCAAAATCGTTCCCGACCATGA
Protein sequence

>MS.gene022567.t1

MGEKDDEFSLGLSLSLGFGEYANNNNQTPLKVTHMHKPPQTVTNQRVSFNNLFHFHDLSTETRSFLRGIDGNTPLPSMAATAAAFDDENGVSSPNSTVSSISGKRSEREGNGDENDAVERGSCSRGGGSDDDDGGGCGGDGDSSRKKLRLSKEQSMLLEETFKEHNTLNPKQKQALAKQLNLKPRQVEVWFQNRRARTKLKQTEVDCEYLKRCCETLTDENRRLQKEVQELRALKLSPQLYMQMNPPTTLTMCPSCERVAVSSASSSSAAAAAPPSASANQSGPSVQRPTPVNPWAAMSIQNRSRP