AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022671

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022671.t1 MTR_8g009020 89.552 335 29 5 1 332 1 332 0.0 560
MS.gene022671.t1 MTR_8g009010 66.049 324 47 8 1 318 1 267 2.57e-133 382
MS.gene022671.t1 MTR_2g104550 50.407 246 113 4 81 325 77 314 5.51e-66 210
MS.gene022671.t1 MTR_2g104490 47.773 247 117 4 76 321 71 306 3.59e-63 203
MS.gene022671.t1 MTR_7g080780 42.391 276 124 4 54 327 78 320 2.99e-60 196
MS.gene022671.t1 MTR_7g080780 42.391 276 124 4 54 327 91 333 4.47e-60 196
MS.gene022671.t1 MTR_4g009540 44.792 288 122 9 55 323 62 331 2.92e-58 191
MS.gene022671.t1 MTR_2g104490 46.610 236 114 4 76 310 71 295 3.23e-57 187
MS.gene022671.t1 MTR_4g009110 44.672 244 127 3 81 323 114 350 8.34e-56 185
MS.gene022671.t1 MTR_4g009540 44.944 267 110 9 55 302 62 310 1.00e-51 173
MS.gene022671.t1 MTR_5g014600 39.286 252 137 4 85 322 85 334 5.23e-49 167
MS.gene022671.t1 MTR_2g104560 45.588 204 87 5 130 326 42 228 8.78e-48 160
MS.gene022671.t1 MTR_4g097940 45.789 190 88 4 134 321 148 324 1.32e-47 163
MS.gene022671.t1 MTR_4g097920 45.789 190 88 4 134 321 133 309 7.42e-47 161
MS.gene022671.t1 MTR_4g097920 45.789 190 88 4 134 321 148 324 1.07e-46 161
MS.gene022671.t1 MTR_2g010450 44.565 184 90 2 137 318 173 346 5.59e-46 160
MS.gene022671.t1 MTR_0246s0020 35.398 339 179 9 10 318 42 370 3.21e-45 158
MS.gene022671.t1 MTR_4g092700 41.631 233 105 5 94 318 117 326 3.57e-45 157
MS.gene022671.t1 MTR_5g014520 44.670 197 98 3 123 318 139 325 4.60e-45 157
MS.gene022671.t1 MTR_2g104530 42.791 215 108 3 109 322 73 273 4.22e-44 152
MS.gene022671.t1 MTR_6g047570 37.013 308 157 9 14 318 54 327 1.50e-43 153
MS.gene022671.t1 MTR_0250s0040 43.005 193 97 4 133 323 80 261 4.20e-42 147
MS.gene022671.t1 MTR_2g104500 41.004 239 114 5 86 323 60 272 6.11e-42 147
MS.gene022671.t1 MTR_0246s0020 35.385 325 170 9 10 304 42 356 2.76e-41 148
MS.gene022671.t1 MTR_2g104650 46.842 190 84 3 134 318 87 264 4.96e-41 145
MS.gene022671.t1 MTR_5g014640 34.495 287 157 7 44 322 60 323 8.34e-41 145
MS.gene022671.t1 MTR_8g069740 49.412 170 75 5 134 301 105 265 1.22e-40 143
MS.gene022671.t1 MTR_4g066460 38.000 200 112 3 123 322 137 324 1.88e-37 137
MS.gene022671.t1 MTR_0011s0210 40.526 190 99 4 140 325 182 361 1.95e-37 137
MS.gene022671.t1 MTR_4g067010 36.134 238 131 5 96 332 109 326 2.27e-37 136
MS.gene022671.t1 MTR_2g104590 43.229 192 68 6 134 318 62 219 1.67e-36 131
MS.gene022671.t1 MTR_0011s0260 41.436 181 95 4 140 319 182 352 1.20e-35 132
MS.gene022671.t1 MTR_5g014560 39.623 212 114 6 114 324 107 305 1.80e-34 128
MS.gene022671.t1 MTR_4g097950 39.062 256 135 9 77 323 7 250 4.26e-33 123
MS.gene022671.t1 MTR_4g066380 33.333 249 143 4 77 322 93 321 5.41e-30 117
MS.gene022671.t1 MTR_0246s0050 30.894 246 139 6 77 318 94 312 8.35e-30 116
MS.gene022671.t1 MTR_4g098035 30.894 246 139 6 77 318 115 333 1.17e-29 116
MS.gene022671.t1 MTR_7g451650 38.710 155 76 2 164 318 57 192 3.06e-26 103
MS.gene022671.t1 MTR_4g009420 36.774 155 71 2 164 318 65 192 3.18e-25 100
MS.gene022671.t1 MTR_4g009510 40.120 167 79 5 153 318 54 200 3.53e-24 98.2
MS.gene022671.t1 MTR_7g050350 37.838 148 73 2 171 318 2 130 1.17e-23 94.7
MS.gene022671.t1 MTR_7g451650 37.838 148 73 2 171 318 2 130 1.17e-23 94.7
MS.gene022671.t1 MTR_7g451650 37.063 143 71 2 164 306 57 180 8.66e-21 88.6
MS.gene022671.t1 MTR_6g047550 40.426 141 76 3 75 212 110 245 9.13e-20 87.8
MS.gene022671.t1 MTR_4g009420 34.965 143 66 2 164 306 65 180 1.25e-19 85.9
MS.gene022671.t1 MTR_5g014550 36.842 133 73 3 170 301 53 175 5.28e-16 75.9
MS.gene022671.t1 MTR_8g067280 28.655 171 120 1 118 286 439 609 1.12e-13 72.4
MS.gene022671.t1 MTR_1g072320 25.828 151 91 3 141 281 470 609 1.62e-11 65.5
MS.gene022671.t1 MTR_5g030430 26.316 171 124 1 118 286 470 640 2.75e-11 65.1
MS.gene022671.t1 MTR_7g083900 27.389 157 111 2 143 296 288 444 2.89e-11 64.7
MS.gene022671.t1 MTR_7g083900 27.389 157 111 2 143 296 288 444 2.89e-11 64.7
MS.gene022671.t1 MTR_8g024790 23.602 161 115 2 126 286 276 428 4.46e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022671.t1 AT4G37850 45.685 197 95 4 127 321 138 324 2.52e-49 168
MS.gene022671.t1 AT4G37850 45.685 197 95 4 127 321 138 324 2.52e-49 168
MS.gene022671.t1 AT2G22770 41.624 197 109 2 133 323 124 320 8.16e-43 150
MS.gene022671.t1 AT2G22760 43.407 182 92 4 140 318 118 291 2.91e-39 140
MS.gene022671.t1 AT2G22750 42.041 245 129 6 82 321 65 301 3.22e-39 140
MS.gene022671.t1 AT2G22750 42.041 245 129 6 82 321 93 329 4.03e-39 141
MS.gene022671.t1 AT2G22750 42.041 245 129 6 82 321 89 325 4.90e-39 141
MS.gene022671.t1 AT2G22750 42.041 245 128 7 82 321 65 300 9.46e-38 137
MS.gene022671.t1 AT2G22750 42.041 245 128 7 82 321 93 328 1.23e-37 137
MS.gene022671.t1 AT2G22760 42.424 165 85 3 140 302 118 274 8.24e-34 126
MS.gene022671.t1 AT1G63650 27.586 174 112 5 117 279 382 552 3.60e-12 67.8
MS.gene022671.t1 AT1G63650 27.586 174 112 5 117 279 382 552 3.60e-12 67.8
MS.gene022671.t1 AT1G63650 27.586 174 112 5 117 279 382 552 3.60e-12 67.8
MS.gene022671.t1 AT1G32640 28.649 185 108 4 113 287 422 592 7.07e-12 66.6
MS.gene022671.t1 AT4G29930 26.220 164 103 2 142 305 55 200 1.24e-11 63.9
MS.gene022671.t1 AT4G17880 25.123 203 141 3 84 286 361 552 1.38e-11 65.9
MS.gene022671.t1 AT3G26744 27.564 156 112 1 142 296 308 463 1.39e-11 65.5
MS.gene022671.t1 AT3G26744 27.564 156 112 1 142 296 308 463 1.48e-11 65.5
MS.gene022671.t1 AT3G26744 27.564 156 112 1 142 296 308 463 1.48e-11 65.5
MS.gene022671.t1 AT3G26744 27.564 156 112 1 142 296 308 463 1.48e-11 65.5
MS.gene022671.t1 AT2G46510 28.302 159 87 3 123 281 377 508 1.53e-11 65.9
MS.gene022671.t1 AT4G09820 29.452 146 70 4 142 286 364 477 1.54e-11 65.5
MS.gene022671.t1 AT5G46760 24.457 184 125 4 108 286 381 555 2.93e-11 64.7
MS.gene022671.t1 AT2G16910 29.032 155 97 4 143 284 316 470 5.40e-11 63.9
MS.gene022671.t1 AT4G29930 25.568 176 118 3 142 305 55 229 5.58e-11 62.4
MS.gene022671.t1 AT4G29930 26.347 167 102 3 142 305 55 203 7.68e-11 61.6
MS.gene022671.t1 AT5G41315 25.000 184 112 6 117 279 420 598 9.32e-11 63.2

Find 55 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACATGTGACACTATT+AGG 0.173209 8.4:-78853269 None:intergenic
AGAGTTTCTTCTTAGATTCT+TGG 0.212913 8.4:-78853363 None:intergenic
AGTAGCAGTGTCTTGCCTTT+TGG 0.235787 8.4:+78855911 MS.gene022671:CDS
GGATATTGTGGTCAGATTAA+TGG 0.259915 8.4:+78855856 MS.gene022671:CDS
TGTCACATGTTCTTCTATTA+TGG 0.264783 8.4:+78853277 MS.gene022671:CDS
ACATTATGGCTGAGAGAAAA+AGG 0.316242 8.4:+78853402 MS.gene022671:CDS
CCTTCTTAGAATAATATCTA+TGG 0.346102 8.4:-78855763 None:intergenic
CTTGTCTATTATGCAAGCTT+GGG 0.346956 8.4:-78855693 None:intergenic
CCTTGTCTATTATGCAAGCT+TGG 0.350284 8.4:-78855694 None:intergenic
AATATTGTTAAAATATTGTA+TGG 0.351091 8.4:-78853081 None:intergenic
TGGTGACAAATCTCCCTCTT+TGG 0.360825 8.4:+78852983 MS.gene022671:CDS
AAGAACATGTGACACTATTA+GGG 0.364487 8.4:-78853268 None:intergenic
AGAATGTTCTCTTGAATTGT+GGG 0.399894 8.4:-78853125 None:intergenic
ACTCACAATATTGTTGTTCA+AGG 0.402220 8.4:-78853312 None:intergenic
AGCGTGAATGATCTAGTTAA+TGG 0.408638 8.4:+78857128 MS.gene022671:CDS
AGAGATACAAGATCACATTA+TGG 0.415532 8.4:+78853388 MS.gene022671:CDS
GAGAATGTTCTCTTGAATTG+TGG 0.419754 8.4:-78853126 None:intergenic
TTAACTAACCTTCTTCAATC+CGG 0.424112 8.4:-78853471 None:intergenic
ATAATGATAATATTTCTTAG+AGG 0.424597 8.4:+78857183 MS.gene022671:CDS
CATTTACATATAGACTGATA+AGG 0.479352 8.4:+78855559 MS.gene022671:intron
ATGTGAAACAACTTCAAGAA+AGG 0.479598 8.4:+78855609 MS.gene022671:CDS
AACCTTCTTCAATCCGGGTA+TGG 0.482099 8.4:-78853465 None:intergenic
AGCTCGAGAACCATAACAAA+AGG 0.486335 8.4:+78855639 MS.gene022671:CDS
TAACTAACCTTCTTCAATCC+GGG 0.489242 8.4:-78853470 None:intergenic
TTTAGAGTGGATGTTCCAAA+AGG 0.493560 8.4:-78855926 None:intergenic
ATGGACAAGGAAATTCTCAT+TGG 0.497384 8.4:+78855812 MS.gene022671:CDS
GATAGCTTCTTGAAGTATGT+AGG 0.500256 8.4:-78855582 None:intergenic
ATGACTCTTGCTTCAACCCT+AGG 0.502899 8.4:-78855791 None:intergenic
TTCTAAGAAGGAACTACCTA+GGG 0.514151 8.4:+78855775 MS.gene022671:CDS
AATTATGTTCTTGAGAAATG+AGG 0.519126 8.4:-78853172 None:intergenic
GCATTGTCTGCCACCATACC+CGG 0.522189 8.4:+78853452 MS.gene022671:CDS
TGGCCTGCGGCAAAATCTCA+TGG 0.524286 8.4:+78857148 MS.gene022671:CDS
TGTGAAACAACTTCAAGAAA+GGG 0.529417 8.4:+78855610 MS.gene022671:CDS
CACCATACCCGGATTGAAGA+AGG 0.530151 8.4:+78853463 MS.gene022671:CDS
AATCCATTGTGAGAAACAAA+AGG 0.531350 8.4:+78855835 MS.gene022671:CDS
ATTATGTTCTTGAGAAATGA+GGG 0.546811 8.4:-78853171 None:intergenic
CCATAGATATTATTCTAAGA+AGG 0.551784 8.4:+78855763 MS.gene022671:CDS
TTCTTGAGAAATGAGGGAGT+TGG 0.554351 8.4:-78853165 None:intergenic
AAAAGTTACAATCATTGCCC+AGG 0.561481 8.4:+78855946 MS.gene022671:CDS
ATTCTAAGAAGGAACTACCT+AGG 0.562025 8.4:+78855774 MS.gene022671:CDS
TGAGAAACAAAAGGATATTG+TGG 0.568495 8.4:+78855844 MS.gene022671:CDS
AAACTTGTCTTCATCCAAAG+AGG 0.569979 8.4:-78852997 None:intergenic
GGAGACAAGTTCTTAGTGAG+AGG 0.572609 8.4:+78853423 MS.gene022671:CDS
GAATGTTCTCTTGAATTGTG+GGG 0.574490 8.4:-78853124 None:intergenic
CTTCTTCAATCCGGGTATGG+TGG 0.579713 8.4:-78853462 None:intergenic
CCAAGCTTGCATAATAGACA+AGG 0.581224 8.4:+78855694 MS.gene022671:CDS
AAAGACAAAATGTATTGAGA+AGG 0.581821 8.4:-78853221 None:intergenic
TAGGGTTGAAGCAAGAGTCA+TGG 0.596540 8.4:+78855793 MS.gene022671:CDS
AACTTGTCTTCATCCAAAGA+GGG 0.612368 8.4:-78852996 None:intergenic
TCCATGTTATAGGAAATGGT+TGG 0.616814 8.4:+78852963 None:intergenic
CATGTGACACTATTAGGGGA+AGG 0.617663 8.4:-78853263 None:intergenic
AATGTTCTCTTGAATTGTGG+GGG 0.666781 8.4:-78853123 None:intergenic
ATGATCTAGTTAATGGCCTG+CGG 0.675515 8.4:+78857135 MS.gene022671:CDS
TGAAGCAAGAGTCATGGACA+AGG 0.676606 8.4:+78855799 MS.gene022671:CDS
AGAACATGTGACACTATTAG+GGG 0.723595 8.4:-78853267 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAAATAATAATAAAAT+TGG - chr8.4:78855272-78855291 None:intergenic 0.0%
!! AATATTGTTAAAATATTGTA+TGG - chr8.4:78853084-78853103 None:intergenic 10.0%
!! TTATTATTTCAATTGTATAA+AGG - chr8.4:78856800-78856819 None:intergenic 10.0%
!!! TTTTGATGATTTTATTTTTA+AGG + chr8.4:78856333-78856352 MS.gene022671:intron 10.0%
!!! TTTTTTTTTTACCAAATAAT+GGG - chr8.4:78853782-78853801 None:intergenic 10.0%
!!! TTTTTTTTTTTACCAAATAA+TGG - chr8.4:78853783-78853802 None:intergenic 10.0%
!! ATATTGTTTAATTATTACCT+GGG - chr8.4:78855966-78855985 None:intergenic 15.0%
!! ATTATTCAAATATTACTCTT+TGG - chr8.4:78856383-78856402 None:intergenic 15.0%
!! GAATTAAGAGAAATTTAATA+TGG + chr8.4:78856686-78856705 MS.gene022671:intron 15.0%
!! TAACTAAATGTATCTTTATA+CGG - chr8.4:78856429-78856448 None:intergenic 15.0%
!! TAGTAATTTACAACATAAAA+TGG + chr8.4:78853903-78853922 MS.gene022671:intron 15.0%
!! TATATTGTTTAATTATTACC+TGG - chr8.4:78855967-78855986 None:intergenic 15.0%
!! TATTCAAAATTAAAGAACTT+TGG - chr8.4:78856919-78856938 None:intergenic 15.0%
!! TTAATTAAAATGTGTATGTT+TGG - chr8.4:78854456-78854475 None:intergenic 15.0%
!! TTATATATCAATGATATTGA+GGG - chr8.4:78853511-78853530 None:intergenic 15.0%
!! TTTATATATCAATGATATTG+AGG - chr8.4:78853512-78853531 None:intergenic 15.0%
!!! AAAAAAAACATATTCTGAAA+AGG + chr8.4:78854926-78854945 MS.gene022671:intron 15.0%
!!! ATTTCATTTTCCAAAATAAT+TGG + chr8.4:78855992-78856011 MS.gene022671:intron 15.0%
!!! GTTTTAATCAAAATATAAGA+AGG + chr8.4:78856891-78856910 MS.gene022671:intron 15.0%
!!! TAAGCAAAAAGTTATTTTTT+AGG + chr8.4:78854715-78854734 MS.gene022671:intron 15.0%
!! AAAGTTATATAAAGAACACA+AGG - chr8.4:78855491-78855510 None:intergenic 20.0%
!! AATAAATTATTGAACACATG+TGG - chr8.4:78853589-78853608 None:intergenic 20.0%
!! AATAATCAAAAACAACCTTT+AGG + chr8.4:78856397-78856416 MS.gene022671:intron 20.0%
!! AATCATCAAAAAAAGAAGAA+AGG - chr8.4:78856324-78856343 None:intergenic 20.0%
!! AATGTATCTAGTCTATATAT+AGG - chr8.4:78854766-78854785 None:intergenic 20.0%
!! GAAAATACTAGAATAGATAT+TGG + chr8.4:78853823-78853842 MS.gene022671:intron 20.0%
!! GAAATTGTTTCAATAAAATG+TGG - chr8.4:78856022-78856041 None:intergenic 20.0%
!! GTTCATAAAACTAATTGAAA+GGG - chr8.4:78853660-78853679 None:intergenic 20.0%
!!! AAGCATATTTAGAATTTCAA+AGG - chr8.4:78857071-78857090 None:intergenic 20.0%
!!! AAGGACTATATACTTATTTT+TGG - chr8.4:78857052-78857071 None:intergenic 20.0%
!!! ACCACTTTTTTTTTTCAAAA+AGG + chr8.4:78854614-78854633 MS.gene022671:intron 20.0%
!!! AGTTTTTGTCAAAATTATGA+CGG - chr8.4:78855121-78855140 None:intergenic 20.0%
!!! ATTTCTTTATTGGTTGTTAA+CGG - chr8.4:78854151-78854170 None:intergenic 20.0%
!!! TCCTTTTTGAAAAAAAAAAG+TGG - chr8.4:78854618-78854637 None:intergenic 20.0%
!!! TTTTTTTTTTCTGTGTACAA+TGG + chr8.4:78853690-78853709 MS.gene022671:intron 20.0%
! AAAATTGGCTAGTAATATTG+AGG - chr8.4:78855257-78855276 None:intergenic 25.0%
! AAAGACAAAATGTATTGAGA+AGG - chr8.4:78853224-78853243 None:intergenic 25.0%
! AAATTATTTCAAGATGACCA+TGG - chr8.4:78856294-78856313 None:intergenic 25.0%
! AAATTGGCTAGTAATATTGA+GGG - chr8.4:78855256-78855275 None:intergenic 25.0%
! AAGGTTTCAATGATATGATT+CGG - chr8.4:78854036-78854055 None:intergenic 25.0%
! AATTATGTTCTTGAGAAATG+AGG - chr8.4:78853175-78853194 None:intergenic 25.0%
! ACACAATACGATTTCTTTAT+TGG - chr8.4:78854161-78854180 None:intergenic 25.0%
! ATATAAAGAACACAAGGTAA+AGG - chr8.4:78855485-78855504 None:intergenic 25.0%
! ATGTACGAGTTTCATAAAAA+AGG - chr8.4:78856650-78856669 None:intergenic 25.0%
! ATTATGTTCTTGAGAAATGA+GGG - chr8.4:78853174-78853193 None:intergenic 25.0%
! CATTTACATATAGACTGATA+AGG + chr8.4:78855559-78855578 MS.gene022671:intron 25.0%
! CCTTCTTAGAATAATATCTA+TGG - chr8.4:78855766-78855785 None:intergenic 25.0%
! GGTTCATAAAACTAATTGAA+AGG - chr8.4:78853661-78853680 None:intergenic 25.0%
! TTAAAACCATCAATTGATGT+CGG - chr8.4:78856878-78856897 None:intergenic 25.0%
! TTATATCATTGTCAGAATCA+CGG + chr8.4:78855091-78855110 MS.gene022671:intron 25.0%
!! AATCTCTTTTCCTTTTGTTA+TGG - chr8.4:78855652-78855671 None:intergenic 25.0%
!! CCATAGATATTATTCTAAGA+AGG + chr8.4:78855763-78855782 MS.gene022671:CDS 25.0%
!! GTTCTTTTCTTAATTGTCTT+TGG - chr8.4:78853344-78853363 None:intergenic 25.0%
!! TCATTTTCCAAAATAATTGG+AGG + chr8.4:78855995-78856014 MS.gene022671:intron 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG + chr8.4:78854813-78854832 MS.gene022671:intron 25.0%
!!! AATGATTGTAACTTTTAGAG+TGG - chr8.4:78855942-78855961 None:intergenic 25.0%
!!! ACTAGATATTTTAATGACGA+TGG - chr8.4:78856949-78856968 None:intergenic 25.0%
AAGAACATGTGACACTATTA+GGG - chr8.4:78853271-78853290 None:intergenic 30.0%
AATAGATATTGGAATGAGCT+TGG + chr8.4:78853834-78853853 MS.gene022671:intron 30.0%
AATCCATTGTGAGAAACAAA+AGG + chr8.4:78855835-78855854 MS.gene022671:CDS 30.0%
ACAAAGAATAATCGATGTCA+TGG - chr8.4:78855061-78855080 None:intergenic 30.0%
AGAATGTTCTCTTGAATTGT+GGG - chr8.4:78853128-78853147 None:intergenic 30.0%
AGAGATACAAGATCACATTA+TGG + chr8.4:78853388-78853407 MS.gene022671:CDS 30.0%
AGAGTTTCTTCTTAGATTCT+TGG - chr8.4:78853366-78853385 None:intergenic 30.0%
ATAGATATTGGAATGAGCTT+GGG + chr8.4:78853835-78853854 MS.gene022671:intron 30.0%
ATGTGAAACAACTTCAAGAA+AGG + chr8.4:78855609-78855628 MS.gene022671:CDS 30.0%
CATAAGAAAGAAAGTGAGTT+AGG + chr8.4:78854273-78854292 MS.gene022671:intron 30.0%
GGAAACGAAGAGAAAAAAAA+AGG - chr8.4:78856573-78856592 None:intergenic 30.0%
GGTAACAGTACATACAAATA+TGG + chr8.4:78856502-78856521 MS.gene022671:intron 30.0%
GTTTAAGGACCATTATTATG+AGG - chr8.4:78856594-78856613 None:intergenic 30.0%
TATCATTGAAACCTTGATCA+AGG + chr8.4:78854041-78854060 MS.gene022671:intron 30.0%
TCTTCGTTTCCTCATAATAA+TGG + chr8.4:78856582-78856601 MS.gene022671:intron 30.0%
TGACATTGTGAATGATTGAT+TGG - chr8.4:78854569-78854588 None:intergenic 30.0%
TGAGAAACAAAAGGATATTG+TGG + chr8.4:78855844-78855863 MS.gene022671:CDS 30.0%
TGCTTAACCTATATCTTAGT+TGG + chr8.4:78855219-78855238 MS.gene022671:intron 30.0%
TGTCACATGTTCTTCTATTA+TGG + chr8.4:78853277-78853296 MS.gene022671:CDS 30.0%
TGTGAAACAACTTCAAGAAA+GGG + chr8.4:78855610-78855629 MS.gene022671:CDS 30.0%
TTAACTAACCTTCTTCAATC+CGG - chr8.4:78853474-78853493 None:intergenic 30.0%
! ACAATCATGTTTCTCTTTTG+AGG + chr8.4:78853037-78853056 MS.gene022671:CDS 30.0%
! ACTCACAATATTGTTGTTCA+AGG - chr8.4:78853315-78853334 None:intergenic 30.0%
! CAATCATGTTTCTCTTTTGA+GGG + chr8.4:78853038-78853057 MS.gene022671:CDS 30.0%
! TTTTGGTACAAATCATCGTA+GGG - chr8.4:78857035-78857054 None:intergenic 30.0%
!! GATAAAGTGCTGAAATGAAA+AGG - chr8.4:78855012-78855031 None:intergenic 30.0%
!! TATCCTTTTGTTTCTCACAA+TGG - chr8.4:78855841-78855860 None:intergenic 30.0%
!! TTTTTGGTACAAATCATCGT+AGG - chr8.4:78857036-78857055 None:intergenic 30.0%
!!! TGTTGTTCAAGGTTGTTTTT+TGG - chr8.4:78853304-78853323 None:intergenic 30.0%
!!! TTGGTTTCACTTTTGAAACA+AGG - chr8.4:78854550-78854569 None:intergenic 30.0%
AAAAGTTACAATCATTGCCC+AGG + chr8.4:78855946-78855965 MS.gene022671:CDS 35.0%
AAACTTGTCTTCATCCAAAG+AGG - chr8.4:78853000-78853019 None:intergenic 35.0%
AACTTGTCTTCATCCAAAGA+GGG - chr8.4:78852999-78853018 None:intergenic 35.0%
AATCGTATTGTGTGAGAGAA+TGG + chr8.4:78854168-78854187 MS.gene022671:intron 35.0%
AATGTTCTCTTGAATTGTGG+GGG - chr8.4:78853126-78853145 None:intergenic 35.0%
ACAAAACCTACCCTATTACA+AGG + chr8.4:78854642-78854661 MS.gene022671:intron 35.0%
ACAGTACTTGCAAGAGTTTA+AGG - chr8.4:78856609-78856628 None:intergenic 35.0%
ACATTATGGCTGAGAGAAAA+AGG + chr8.4:78853402-78853421 MS.gene022671:CDS 35.0%
ACCATGTCCAACTAAGATAT+AGG - chr8.4:78855229-78855248 None:intergenic 35.0%
ACCTATATCTTAGTTGGACA+TGG + chr8.4:78855225-78855244 MS.gene022671:intron 35.0%
ACTATGACTTTATGATCGAG+TGG + chr8.4:78853733-78853752 MS.gene022671:intron 35.0%
AGAAAAAAGCTATACTCCTC+TGG + chr8.4:78854683-78854702 MS.gene022671:intron 35.0%
AGAACATGTGACACTATTAG+GGG - chr8.4:78853270-78853289 None:intergenic 35.0%
AGATAGGAGAAAAGCTGAAA+AGG + chr8.4:78856231-78856250 MS.gene022671:intron 35.0%
AGCGTGAATGATCTAGTTAA+TGG + chr8.4:78857128-78857147 MS.gene022671:CDS 35.0%
ATAGGAGAAAAGCTGAAAAG+GGG + chr8.4:78856233-78856252 MS.gene022671:intron 35.0%
ATCGTATTGTGTGAGAGAAT+GGG + chr8.4:78854169-78854188 MS.gene022671:intron 35.0%
ATGGACAAGGAAATTCTCAT+TGG + chr8.4:78855812-78855831 MS.gene022671:CDS 35.0%
ATTGAACCGACATCAATTGA+TGG + chr8.4:78856869-78856888 MS.gene022671:intron 35.0%
CAAAACCTACCCTATTACAA+GGG + chr8.4:78854643-78854662 MS.gene022671:intron 35.0%
CCTTTCAGAAACATGTTATG+TGG - chr8.4:78854901-78854920 None:intergenic 35.0%
CTCGATCATAAAGTCATAGT+TGG - chr8.4:78853733-78853752 None:intergenic 35.0%
CTTGCTTACTACCCATTATT+TGG + chr8.4:78853768-78853787 MS.gene022671:intron 35.0%
CTTGTCTATTATGCAAGCTT+GGG - chr8.4:78855696-78855715 None:intergenic 35.0%
CTTTAGCTATATCATGCAGA+TGG + chr8.4:78857088-78857107 MS.gene022671:intron 35.0%
GAAGAACATGTGACACTATT+AGG - chr8.4:78853272-78853291 None:intergenic 35.0%
GAATGTTCTCTTGAATTGTG+GGG - chr8.4:78853127-78853146 None:intergenic 35.0%
GAGAATGTTCTCTTGAATTG+TGG - chr8.4:78853129-78853148 None:intergenic 35.0%
GATAGCTTCTTGAAGTATGT+AGG - chr8.4:78855585-78855604 None:intergenic 35.0%
GATAGGAGAAAAGCTGAAAA+GGG + chr8.4:78856232-78856251 MS.gene022671:intron 35.0%
GGATATTGTGGTCAGATTAA+TGG + chr8.4:78855856-78855875 MS.gene022671:CDS 35.0%
TAACTAACCTTCTTCAATCC+GGG - chr8.4:78853473-78853492 None:intergenic 35.0%
TAGACCACTTATAATGAACG+TGG - chr8.4:78856713-78856732 None:intergenic 35.0%
TCATCTTTATAAGTGGTCTG+TGG - chr8.4:78856998-78857017 None:intergenic 35.0%
TTAGAAAAAGAGAGCTTCCA+TGG + chr8.4:78856274-78856293 MS.gene022671:intron 35.0%
TTCAGAAACATGTTATGTGG+TGG - chr8.4:78854898-78854917 None:intergenic 35.0%
TTGCTTATAAATGTGACCAG+AGG - chr8.4:78854702-78854721 None:intergenic 35.0%
TTTATACGGATGTGTCCTAA+AGG - chr8.4:78856415-78856434 None:intergenic 35.0%
TTTGGTACAAATCATCGTAG+GGG - chr8.4:78857034-78857053 None:intergenic 35.0%
! ATGTGGACCTCCAATTATTT+TGG - chr8.4:78856005-78856024 None:intergenic 35.0%
! ATTCTAAGAAGGAACTACCT+AGG + chr8.4:78855774-78855793 MS.gene022671:CDS 35.0%
! CCACATAACATGTTTCTGAA+AGG + chr8.4:78854898-78854917 MS.gene022671:intron 35.0%
! GTGACGCTTTGTTAAAATTG+AGG - chr8.4:78854855-78854874 None:intergenic 35.0%
! TTCTAAGAAGGAACTACCTA+GGG + chr8.4:78855775-78855794 MS.gene022671:CDS 35.0%
! TTTAGAGTGGATGTTCCAAA+AGG - chr8.4:78855929-78855948 None:intergenic 35.0%
AATGAGTGTGACAAGAGAGA+GGG + chr8.4:78856202-78856221 MS.gene022671:intron 40.0%
ACCTCAGTTCCCTTGTAATA+GGG - chr8.4:78854655-78854674 None:intergenic 40.0%
AGAGAGAGGGAAAACAAGAT+AGG + chr8.4:78856215-78856234 MS.gene022671:intron 40.0%
AGCTCGAGAACCATAACAAA+AGG + chr8.4:78855639-78855658 MS.gene022671:CDS 40.0%
ATCACCACATTCCTTGATCA+AGG - chr8.4:78854055-78854074 None:intergenic 40.0%
ATCGATGTCATGGTAGTATG+TGG - chr8.4:78855051-78855070 None:intergenic 40.0%
ATGATCTAGTTAATGGCCTG+CGG + chr8.4:78857135-78857154 MS.gene022671:CDS 40.0%
CAATTAGCAGTTTGGCATGA+TGG - chr8.4:78854977-78854996 None:intergenic 40.0%
CAGCAGGTCATCTTTATAAG+TGG - chr8.4:78857005-78857024 None:intergenic 40.0%
CCAAGCTTGCATAATAGACA+AGG + chr8.4:78855694-78855713 MS.gene022671:CDS 40.0%
CCTTGTCTATTATGCAAGCT+TGG - chr8.4:78855697-78855716 None:intergenic 40.0%
CTTCAACTAAAAATGCGTCG+TGG - chr8.4:78854381-78854400 None:intergenic 40.0%
GAAACCTTGATCAAGGAATG+TGG + chr8.4:78854048-78854067 MS.gene022671:intron 40.0%
GAGAGAATGGGTGTAAACAA+AGG + chr8.4:78854181-78854200 MS.gene022671:intron 40.0%
GAGCATGACAATTAGCAGTT+TGG - chr8.4:78854985-78855004 None:intergenic 40.0%
TGCTTATAAATGTGACCGGA+GGG + chr8.4:78854817-78854836 MS.gene022671:intron 40.0%
TGGACCACGTTCATTATAAG+TGG + chr8.4:78856706-78856725 MS.gene022671:intron 40.0%
TGGCTAGTAATATTGAGGGA+TGG - chr8.4:78855252-78855271 None:intergenic 40.0%
TTCTTGAGAAATGAGGGAGT+TGG - chr8.4:78853168-78853187 None:intergenic 40.0%
TTGCTTATAAATGTGACCGG+AGG + chr8.4:78854816-78854835 MS.gene022671:intron 40.0%
! AGTGCTGAAATGAAAAGGAG+AGG - chr8.4:78855007-78855026 None:intergenic 40.0%
AACCTTCTTCAATCCGGGTA+TGG - chr8.4:78853468-78853487 None:intergenic 45.0%
ACCCTATTACAAGGGAACTG+AGG + chr8.4:78854651-78854670 MS.gene022671:intron 45.0%
ATGACTCTTGCTTCAACCCT+AGG - chr8.4:78855794-78855813 None:intergenic 45.0%
ATTACAAGGGAACTGAGGTC+TGG + chr8.4:78854656-78854675 MS.gene022671:intron 45.0%
CAGTTCCCTTGTAATAGGGT+AGG - chr8.4:78854651-78854670 None:intergenic 45.0%
CATGTGACACTATTAGGGGA+AGG - chr8.4:78853266-78853285 None:intergenic 45.0%
GAATGAGTGTGACAAGAGAG+AGG + chr8.4:78856201-78856220 MS.gene022671:intron 45.0%
GACCTCAGTTCCCTTGTAAT+AGG - chr8.4:78854656-78854675 None:intergenic 45.0%
GGAGACAAGTTCTTAGTGAG+AGG + chr8.4:78853423-78853442 MS.gene022671:CDS 45.0%
GTGTACAATGGTACGTAGCA+AGG + chr8.4:78853702-78853721 MS.gene022671:intron 45.0%
TACAATGGTACGTAGCAAGG+AGG + chr8.4:78853705-78853724 MS.gene022671:intron 45.0%
TAGGGTTGAAGCAAGAGTCA+TGG + chr8.4:78855793-78855812 MS.gene022671:CDS 45.0%
TGAAGCAAGAGTCATGGACA+AGG + chr8.4:78855799-78855818 MS.gene022671:CDS 45.0%
TGGTGACAAATCTCCCTCTT+TGG + chr8.4:78852983-78853002 MS.gene022671:CDS 45.0%
! AGGTTCTTTTGTACTCCCTC+CGG - chr8.4:78854835-78854854 None:intergenic 45.0%
! AGTAGCAGTGTCTTGCCTTT+TGG + chr8.4:78855911-78855930 MS.gene022671:CDS 45.0%
! AGTTTGGCATGATGGTGAAG+AGG - chr8.4:78854969-78854988 None:intergenic 45.0%
! ATTCCATGAGATTTTGCCGC+AGG - chr8.4:78857154-78857173 None:intergenic 45.0%
! GTTTGGCATGATGGTGAAGA+GGG - chr8.4:78854968-78854987 None:intergenic 45.0%
!! AAAAAGAATTATTATTAAAT+TGG + chr8.4:78856481-78856500 MS.gene022671:intron 5.0%
!! AGAAAAAAAAAAAAAAAAAA+AGG - chr8.4:78853682-78853701 None:intergenic 5.0%
!!! GTAATATTAAAATAATTTTT+TGG + chr8.4:78856078-78856097 MS.gene022671:intron 5.0%
!!! TATTTATTTTTTATTAAAGT+TGG - chr8.4:78855391-78855410 None:intergenic 5.0%
CACCATACCCGGATTGAAGA+AGG + chr8.4:78853463-78853482 MS.gene022671:CDS 50.0%
CTTCTTCAATCCGGGTATGG+TGG - chr8.4:78853465-78853484 None:intergenic 50.0%
! ATCGTAGGGGAGTTTTCAGC+AGG - chr8.4:78857021-78857040 None:intergenic 50.0%
GCATTGTCTGCCACCATACC+CGG + chr8.4:78853452-78853471 MS.gene022671:CDS 55.0%
TGGCCTGCGGCAAAATCTCA+TGG + chr8.4:78857148-78857167 MS.gene022671:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 78852978 78857202 78852978 ID=MS.gene022671
chr8.4 mRNA 78852978 78857202 78852978 ID=MS.gene022671.t1;Parent=MS.gene022671
chr8.4 exon 78852978 78853484 78852978 ID=MS.gene022671.t1.exon1;Parent=MS.gene022671.t1
chr8.4 CDS 78852978 78853484 78852978 ID=cds.MS.gene022671.t1;Parent=MS.gene022671.t1
chr8.4 exon 78855572 78855967 78855572 ID=MS.gene022671.t1.exon2;Parent=MS.gene022671.t1
chr8.4 CDS 78855572 78855967 78855572 ID=cds.MS.gene022671.t1;Parent=MS.gene022671.t1
chr8.4 exon 78857107 78857202 78857107 ID=MS.gene022671.t1.exon3;Parent=MS.gene022671.t1
chr8.4 CDS 78857107 78857202 78857107 ID=cds.MS.gene022671.t1;Parent=MS.gene022671.t1
Gene Sequence

>MS.gene022671

ATGGTTGGTGACAAATCTCCCTCTTTGGATGAAGACAAGTTTCAACAAGAGAATATGCAACAATCATGTTTCTCTTTTGAGGGTGATAATTCTCAATCTTCAACCATACAATATTTTAACAATATTTTAGATAATGTTTGTGTCACCCCCACAATTCAAGAGAACATTCTCAAGAATAGCAATAGTTCCAACTCCCTCATTTCTCAAGAACATAATTGTGCTACTAATTCACAAAAATTAGCACCTTCTCAATACATTTTGTCTTTTGAAAACTCAAGTGTGAAACCTTCCCCTAATAGTGTCACATGTTCTTCTATTATGGTCCAAAAAACAACCTTGAACAACAATATTGTGAGTGAATTACCAAAGACAATTAAGAAAAGAACCAAGAATCTAAGAAGAAACTCTTCAGAGATACAAGATCACATTATGGCTGAGAGAAAAAGGAGACAAGTTCTTAGTGAGAGGTTCATAGCATTGTCTGCCACCATACCCGGATTGAAGAAGGTTAGTTAATTTCTTATGTTAAACCCTCAATATCATTGATATATAAATCATAGATTCATCATTTTTTTTTTAAGAGAAATCGTAGATTCATCATAATCAAGCCACATGTGTTCAATAATTTATTTCTTATATAACTTTTCAAGTTATTTCAATATCTATTCTAATATCTTCTCCCTTTCAATTAGTTTTATGAACCTTTTTTTTTTTTTTTTTTTCTGTGTACAATGGTACGTAGCAAGGAGGTGCCAACTATGACTTTATGATCGAGTGGTTTGTATGAAGTCTTGCTTACTACCCATTATTTGGTAAAAAAAAAAAATTATAAAACTAATCAAGAAGAAAATACTAGAATAGATATTGGAATGAGCTTGGGAAATTATATAAGAAATAAATTTGTGCTTAATTATATGAAATTACGTAGTAATTTACAACATAAAATGGTTCTCATAAACATCAAATTTCTGAGATTATTAATTCTATGATTTAAAGTTGAATGTTATAGCATTCAATGCAGCAAGCTAGTCATGACTCACGACAGAGACGTTTAACCGAATCATATCATTGAAACCTTGATCAAGGAATGTGGTGATAAAAATTAATTGATCATGTAAATTGATAAAGTAGTTCGTTTTTTTTTTAAACTAGTTCAACTGTAATAATATACCGTTAACAACCAATAAAGAAATCGTATTGTGTGAGAGAATGGGTGTAAACAAAGGTGAGTTAGAGTAATGTGATTTATTTTATTTTTTATTCGAATTGTTAAGTGATTTATATTAGAATATATTCATAAGAAAGAAAGTGAGTTAGGCAAAAACATTTTAGACTTTATTGATGCAAAAGTGCTCTAAATTCTACATTCACTTTAGAATCAATTATACTTGTATTTAGAACCACGACGCATTTTTAGTTGAAGTTATGTCAAATAACTTTTGAGTTTCGATAACTTTTTACATAAACAAGAGTTTCCAAACATACACATTTTAATTAAAAGCAAAATTAAGTCTACTTAAAAATATTTGAATATGTCTAAATCAATTTCATACATTTAAAATTAATCTTCCTTGTTTCAAAAGTGAAACCAATCAATCATTCACAATGTCATTGATGCACACATAATGTGTTAAGAACCACTTTTTTTTTTCAAAAAGGAAAGCACAAAACCTACCCTATTACAAGGGAACTGAGGTCTGGTGTTAGAAAAAAGCTATACTCCTCTGGTCACATTTATAAGCAAAAAGTTATTTTTTAGGTTCATTCAATAACTAATGTATCTAACCTATATATAGACTAGATACATTATTTATTGAATGAACTTAAAAAGTTACTTTTTGCTTATAAATGTGACCGGAGGGAGTACAAAAGAACCTCAATTTTAACAAAGCGTCACTTTCATGCACATACACACATCCACCACATAACATGTTTCTGAAAGGTTTTCAAAAAAAACATATTCTGAAAAGGTCGAATGTACTCTCTTCCCTCTTCACCATCATGCCAAACTGCTAATTGTCATGCTCCTCTCCTTTTCATTTCAGCACTTTATCAAACATGTAAAATCTTCCACATACTACCATGACATCGATTATTCTTTGTTTGTTTTTTATTATATCATTGTCAGAATCACGGTAAACCGTCATAATTTTGACAAAAACTACATTTTAAAGTTTCACAAAATCATATCATTTTGCTTATCTGCATGCATATACTTAATCTAACAACTTGAACAAAATTTGCTTAACCTATATCTTAGTTGGACATGGTCCATCCCTCAATATTACTAGCCAATTTTATTATTATTTTTATAAACTATATTACTTACTTGTTTTTAAAGATAAAAGAAAAATAAAAAAATTGACACATTTATATAAAAAAAATATTATGTATAATAATAATTATACTACCAACTTTAATAAAAAATAAATATTGTGTACAGTTGAATATATTTACGTATCTTTTATCTCTTTTTGCTTTTCTTTTCTATCAATCATAGTATGCCTTTACCTTGTGTTCTTTATATAACTTTATAATAACTTTGAAATTCAAGTATATATAATTCAAATATATATTTTCTCATTTACATATAGACTGATAAGGCCTACATACTTCAAGAAGCTATCAATTATGTGAAACAACTTCAAGAAAGGGTAAATGAGCTCGAGAACCATAACAAAAGGAAAAGAGATTCAGTAATATTCATCAAGAAATCCCAAGCTTGCATAATAGACAAGGAAAAAAGTACTTCATCATGTGAAGAGAATTTTGATAATGATGATGACCATAGATATTATTCTAAGAAGGAACTACCTAGGGTTGAAGCAAGAGTCATGGACAAGGAAATTCTCATTGGAATCCATTGTGAGAAACAAAAGGATATTGTGGTCAGATTAATGGCTTTGCTTCAAAATCTTCATCTATCTCTTGCTAGTAGCAGTGTCTTGCCTTTTGGAACATCCACTCTAAAAGTTACAATCATTGCCCAGGTAATAATTAAACAATATATAAACATTTCATTTTCCAAAATAATTGGAGGTCCACATTTTATTGAAACAATTTCTTGCATTGTATTAACACTTTTTAACTAAAATTTGAAGTAATATTAAAATAATTTTTTGGTTCTAGTATTCAAATTATTTTAATACAAGTTTACATGTTTTTTAAACATAAACTTTAAGAGAAATGTTGTATTATGCAAGACAAAAATGACAAATAATGCGAATGAGTGTGACAAGAGAGAGGGAAAACAAGATAGGAGAAAAGCTGAAAAGGGGAAAAGTGAAAGAAAGTTATTAGAAAAAGAGAGCTTCCATGGTCATCTTGAAATAATTTGAATCGTCCTTTCTTCTTTTTTTGATGATTTTATTTTTAAGGATTCTTTTTTTATGAATGAAAATTCCAAAGAGTAATATTTGAATAATCAAAAACAACCTTTAGGACACATCCGTATAAAGATACATTTAGTTATTTAAAGTAAATTTTAGTTTGACTATGAGAGAAAAAAGAATTATTATTAAATTGGTAACAGTACATACAAATATGGTTTACAACTTTTAAATTGCAAAATAAATTTTCTGTTAAAAAATGACCTTTTTTTTTCTCTTCGTTTCCTCATAATAATGGTCCTTAAACTCTTGCAAGTACTGTGTTCACGTGACAATTGTTCCTTTTTTATGAAACTCGTACATACATGCATATAGTATAGAATTAAGAGAAATTTAATATGGACCACGTTCATTATAAGTGGTCTAGCATAAATTAATATTTTAAAAAAAATATTTAAAACTCTAGATAGTAGATGTTCATGATCATTGTCCTTTATACAATTGAAATAATAACTTAAATGATATCGATTTATTGTTAAAAGAAAAAAAAAGTGTAACTTGTATTGAACCGACATCAATTGATGGTTTTAATCAAAATATAAGAAGGATCCAAAGTTCTTTAATTTTGAATAATATTGTCCATCGTCATTAAAATATCTAGTAATTTTAAGTATTGATTCATGGTGGACCACAGACCACTTATAAAGATGACCTGCTGAAAACTCCCCTACGATGATTTGTACCAAAAATAAGTATATAGTCCTTTGAAATTCTAAATATGCTTTAGCTATATCATGCAGATGGATGATGAATATTGCATGAGCGTGAATGATCTAGTTAATGGCCTGCGGCAAAATCTCATGGAATCACGTGACAATAATGATAATATTTCTTAG
Protein sequence

>MS.gene022671.t1

MVGDKSPSLDEDKFQQENMQQSCFSFEGDNSQSSTIQYFNNILDNVCVTPTIQENILKNSNSSNSLISQEHNCATNSQKLAPSQYILSFENSSVKPSPNSVTCSSIMVQKTTLNNNIVSELPKTIKKRTKNLRRNSSEIQDHIMAERKRRQVLSERFIALSATIPGLKKTDKAYILQEAINYVKQLQERVNELENHNKRKRDSVIFIKKSQACIIDKEKSTSSCEENFDNDDDHRYYSKKELPRVEARVMDKEILIGIHCEKQKDIVVRLMALLQNLHLSLASSSVLPFGTSTLKVTIIAQMDDEYCMSVNDLVNGLRQNLMESRDNNDNIS