AlfalfaGEDB | MS.gene022821

M. sativa cultivar XinJiangDaYe / MS.gene022821

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022821.t1	XP_003626938.2	87	501	46	4	1	496	1	487	4.20E-232	813.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022821.t1	Q9SRT2	42.3	523	196	19	1	494	1	446	3.1e-74	280.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022821.t1	G7LF56	87.0	501	46	4	1	496	1	487	3.0e-232	813.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene022821.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022821.t1	MTR_8g012290	90.800	500	27	4	1	495	1	486	0.0	894
MS.gene022821.t1	MTR_8g012290	90.619	501	27	5	1	495	1	487	0.0	889
MS.gene022821.t1	MTR_7g053410	60.110	544	139	19	1	497	1	513	0.0	548
MS.gene022821.t1	MTR_7g053410	59.963	542	139	19	1	495	1	511	0.0	545
MS.gene022821.t1	MTR_5g048860	34.879	453	203	19	106	503	140	555	2.22e-53	189
MS.gene022821.t1	MTR_8g065740	39.222	334	161	14	105	424	125	430	2.97e-50	179
MS.gene022821.t1	MTR_1g017350	46.058	241	106	8	198	435	171	390	5.81e-48	171
MS.gene022821.t1	MTR_1g017350	46.058	241	106	8	198	435	171	390	6.20e-48	171
MS.gene022821.t1	MTR_3g116770	52.174	207	80	6	219	414	112	310	1.87e-47	169
MS.gene022821.t1	MTR_3g116770	52.174	207	80	6	219	414	112	310	1.91e-47	169

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022821.t1	AT3G07340	43.080	513	180	20	1	480	1	434	8.05e-103	316
MS.gene022821.t1	AT3G07340	42.596	493	172	19	1	460	1	415	1.47e-93	291
MS.gene022821.t1	AT5G48560	42.713	494	193	18	25	483	50	488	2.96e-88	280
MS.gene022821.t1	AT5G48560	42.675	471	181	17	25	460	50	466	8.23e-82	262
MS.gene022821.t1	AT3G23690	45.149	268	104	10	221	479	113	346	5.27e-56	192
MS.gene022821.t1	AT1G68920	40.000	320	140	8	84	388	108	390	1.46e-52	186
MS.gene022821.t1	AT1G68920	40.446	314	148	6	84	388	108	391	1.65e-52	185
MS.gene022821.t1	AT1G68920	40.446	314	148	6	84	388	108	391	1.65e-52	185
MS.gene022821.t1	AT1G68920	40.446	314	148	6	84	388	108	391	1.65e-52	185
MS.gene022821.t1	AT1G26260	43.056	288	115	12	105	390	73	313	3.72e-48	171

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Find 93 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCTTCCAATCCAACAA+AGG	0.664009	8.1:-84777696	MS.gene022821:intron
AAAGAAAATTAAGGCAAATG+AGG	0.506222	8.1:-84777347	MS.gene022821:CDS
AAATGGGACCCACTGCTCAG+TGG	0.666661	8.1:-84776025	MS.gene022821:CDS
AAATGGTATCAAGAATATGG+AGG	0.642262	8.1:-84777311	MS.gene022821:CDS
AAGAAAATTAAGGCAAATGA+GGG	0.561150	8.1:-84777346	MS.gene022821:CDS
AATAACATTCCTAATACAAA+TGG	0.381646	8.1:-84776042	MS.gene022821:CDS
AATACACCTAATGGAGAAAT+TGG	0.254012	8.1:-84777785	MS.gene022821:CDS
AATATTATTATGGCACAAAG+AGG	0.614493	8.1:+84775990	None:intergenic
AATGCATGCCAATATTATTA+TGG	0.315999	8.1:+84775980	None:intergenic
AATTATTCCTTGTAGGTTAC+TGG	0.240542	8.1:-84776488	MS.gene022821:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGGTATCAAGAATATGG+AGG	-	chr8.1:84776502-84776521	MS.gene022821:intron	30.0%
AATACACCTAATGGAGAAAT+TGG	-	chr8.1:84776028-84776047	MS.gene022821:CDS	30.0%
AATTATTCCTTGTAGGTTAC+TGG	-	chr8.1:84777325-84777344	MS.gene022821:CDS	30.0%
ACAATTTAGTCCATACTCTA+TGG	+	chr8.1:84776257-84776276	None:intergenic	30.0%
AGGGAATTGATAGGAAAATT+GGG	-	chr8.1:84775608-84775627	MS.gene022821:intron	30.0%
CAACGGTAATAAAACAGAAA+AGG	-	chr8.1:84778362-84778381	MS.gene022821:CDS	30.0%
CAGTGAAAAAGATCATTAAC+CGG	+	chr8.1:84778047-84778066	None:intergenic	30.0%
CGATGACAAAAAGTCTAAAA+TGG	+	chr8.1:84776180-84776199	None:intergenic	30.0%
CTTCCAAAAACATGATATTG+TGG	-	chr8.1:84777011-84777030	MS.gene022821:intron	30.0%
GCAACAAGAAAATGATAGAA+TGG	-	chr8.1:84775964-84775983	MS.gene022821:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	84775413	84778422	84775413	ID=MS.gene022821
chr8.1	mRNA	84775413	84778422	84775413	ID=MS.gene022821.t1;Parent=MS.gene022821
chr8.1	exon	84777697	84778422	84777697	ID=MS.gene022821.t1.exon1;Parent=MS.gene022821.t1
chr8.1	CDS	84777697	84778422	84777697	ID=cds.MS.gene022821.t1;Parent=MS.gene022821.t1
chr8.1	exon	84777183	84777401	84777183	ID=MS.gene022821.t1.exon2;Parent=MS.gene022821.t1
chr8.1	CDS	84777183	84777401	84777183	ID=cds.MS.gene022821.t1;Parent=MS.gene022821.t1
chr8.1	exon	84776625	84776690	84776625	ID=MS.gene022821.t1.exon3;Parent=MS.gene022821.t1
chr8.1	CDS	84776625	84776690	84776625	ID=cds.MS.gene022821.t1;Parent=MS.gene022821.t1
chr8.1	exon	84776427	84776495	84776427	ID=MS.gene022821.t1.exon4;Parent=MS.gene022821.t1
chr8.1	CDS	84776427	84776495	84776427	ID=cds.MS.gene022821.t1;Parent=MS.gene022821.t1
chr8.1	exon	84776263	84776337	84776263	ID=MS.gene022821.t1.exon5;Parent=MS.gene022821.t1
chr8.1	CDS	84776263	84776337	84776263	ID=cds.MS.gene022821.t1;Parent=MS.gene022821.t1
chr8.1	exon	84775942	84776166	84775942	ID=MS.gene022821.t1.exon6;Parent=MS.gene022821.t1
chr8.1	CDS	84775942	84776166	84775942	ID=cds.MS.gene022821.t1;Parent=MS.gene022821.t1
chr8.1	exon	84775413	84775571	84775413	ID=MS.gene022821.t1.exon7;Parent=MS.gene022821.t1
chr8.1	CDS	84775413	84775571	84775413	ID=cds.MS.gene022821.t1;Parent=MS.gene022821.t1

Gene Sequence

>MS.gene022821

ATGGAAAACCAATTCTTTTTAAATGCTGGATTTGAAACTTACCAAACCCAAGATTGTTTTTTCAACCCAAATTGGGACAAGTCAACGGACCAAAACCTTAACTTTGATTCAGCACTTAGTTCAATTGTCTCATCCCCAGCAGCTACATCATCACCACCAAATCCCAACAACATGTCCAATGAAAATTTTGTGATGAGGGAATTGATAGGAAAATTGGGAACTTTTGGTGAGATCTCACAACATTCTTCTAACCCTTTGGTTGTACCAATGATGAACCATTTTATCAAAGAAAATATGCAAAAACCAATGTCTAATGTGGCAGAATTCTCACATGATCCTGGTTTTGTTCAAAGGGCTGCAAAATTTTCTTGCTTTGGTAGCAAGAGTTTCAATGAAAGAGGTAACCAAATGGTGATGAACAATGTTGACTTAGCTCAAAGATCTCATACTTCAATGGAAAATGGAATGAAGTTATCAAGAGTTTCAAGTAGTCCATCACTCAAAACATTTGGATCTCAAATGATGAATCATGACAACAAGAATTCTCCATTGCAACAAGAAAATGATAGAATGGAAGTGACTAATTCTCAAGAGGAATCTACAATATCTGAGCAAAATACACCTAATGGAGAAATTGGTGTCAAAGCTTCACCTGATATGAATTCAAGGAAGAGAAAAGCTTCTTCTTCCAAAGGAAAAGCACCAAACTCTTCCAATCCAACAAAGGTAAAGCACTAAAGCTGCAATTTTTTAAGAGTGCGGACCCATTTTAGACTTTTTGTCATCGAGACAAACTGCGAGACTCAATTTAGTTCCGACAAAAATTTAAACAAATTTTAGTCCATAGAGTATGGACTAAATTGTTCCTGTTTATTTTTAGACTAAAATATTTTTTTCTTGCAAATTAAATTGAGTGTTGCAATTTTTTCCTGGGACTAAAATATGTTTTTACTTTTTTTTTAAAGTCAATTTTTTTTTTTTATGTTGGAAATTTTCTGAATCTCATTTCTTTTGACTTTAGGGTGTTGAAGGTAGTGGTGAAGACTTAAATGCAAAGAAAATTAAGGCAAATGAGGGTGAGAGAAATGAAAATGGTATCAAGAATATGGAGGAAGAGACCAAAGAAGGGGAAGAAAAACAGAACAAGAGTGACTCAAAACCTCCTGAACCTCAAAAAGATTACATTCATGTCAGAGCAAGAAGAGGTCAAGCTACTGACAGTCACAGTCTTGCAGAACGAGTAAGAATTACTGCTTTACGTTTCCCTATCAATTTTTTTTTTTTTTTTGTTGTGAAATTGGTTGCGTCGCGAGTCACGACCTTTGATATTACATAAAATTATGGATAAATTCGACTTATACAATTGCAGCTTTAATGTAGACTTTCGAGAATTTAAACTTACCGTGTTGCATGCATTCATGCAGCCAACATATTAGTGGTTGTTGAATCGAGATCGGGCAGTTCACATTTTATCATAGACTTTTTATATCTAAAAAAATGAATTTGAAATTTAAACGATTTGATTTCGATTCAACAACCATTGATATGATTACAGTGCAAACGCGTTAAATCGGTGATGGTATAGAATTTGAATTTTACTTCCAAAAACATGATATTGTGGGCCACAATATCCAATATTAGTTGCAAACCTAATTTAAAACATAACAAAAATGTATCAACTTCATATCTTGATTCTTGAGTTGACATTATATGAATCTTTGATTGTAAATAGGTTAGGAGGGAGAAAATAAGTGAGAGGATGAAACTTCTCCAAGATCTTGTACCAGGCTGCAATAAGGTAAACAATACTTTTAAAAGTTACTAAATCAGAATACAAAACAAATTGGTTTAATTTCATGTTACTACTAAAATTTGTACTTTTTCACTAATTTGGCTATGCCTATGAATTATTAATTATTCCTTGTAGGTTACTGGAAAAGCACTTATGCTTGATGAAATTATAAATTATGTTCAGTCATTGCAGCGTCAAGTTGAGGTAATCAACTCTTCACCAATAAGCATCTTAATTCTATAATGTAATTATTTTTGTTTTCTTGCTCATTAAGTTATGGTTTCTTTTTTCAGTTTTTGTCTATGAAATTGTCTTCAGTGAACACAAAGATGGATTTAAGTATTGAAAGTCTTGTTGTATCAAAAGATGTAAGTTTTAAGTCTCTATAGACAATGTTTTGCTTTTTCAATAAAACTTGATGCATTTGTTTATTTATTTATTTATTTTCCTTTTTGTGATTTCAGATATTTCAATCAAATAATTCTTTGCAAAACTCAATGTTCCAATTAGATTCCTCAGTACCATCCTTTTATGGGCAACAACCCCAGCAAAACCAAGCTATTCATAATAACATTCCTAATACAAATGGGACCCACTGCTCAGTGGAGCCATTGGATTCCTCTTTGTGCCATAATAATATTGGCATGCATTTACCTTTCCTAAATGAATTTAATGAAGGTGGTTCTCAGGTAACAAAATTCTTCTACTTTTTTCTATAAATTATCATTAATTATCAATTTAATTTATGCAATTGTTAAGAGTCTAACAATTTGAACTTTAAAAGTATTGAAATTCAAAAATGATTATTAAAATTAAATAAATCAAGTGATTGAGTTCTACCGGTTAATGATCTTTTTCACTGAATTTCAAACTATCAAGCTCGCTATTCCTAGGAACCAGATAATTAAGACAAAAATTTAGGCATCGAAATTATTTTGAAATCTTAAGTATTATTTACATGACCCAATTGTTCCATTGTGTACTAATATATGAAGGTACATTATTTCACTAATGTTTTGAACTTTTCCTAATTAACTCTATTTTTCAAATGATTATCAGTATCCATTAGCATTCTCTGAGGATGACTTAAACACCGTTGTTCAGATGGGATTTGGCCAAACTTCAAACAGGAATACACCAATACACTCCCCAAGTTTCAACGGTAATAAAACAGAAAAGGAAAAAAAAAAAAAAAAAAACAATGTCACAAATTATTGA

Protein sequence

>MS.gene022821.t1

MENQFFLNAGFETYQTQDCFFNPNWDKSTDQNLNFDSALSSIVSSPAATSSPPNPNNMSNENFVMRELIGKLGTFGEISQHSSNPLVVPMMNHFIKENMQKPMSNVAEFSHDPGFVQRAAKFSCFGSKSFNERGNQMVMNNVDLAQRSHTSMENGMKLSRVSSSPSLKTFGSQMMNHDNKNSPLQQENDRMEVTNSQEESTISEQNTPNGEIGVKASPDMNSRKRKASSSKGKAPNSSNPTKGVEGSGEDLNAKKIKANEGERNENGIKNMEEETKEGEEKQNKSDSKPPEPQKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQRQVEFLSMKLSSVNTKMDLSIESLVVSKDIFQSNNSLQNSMFQLDSSVPSFYGQQPQQNQAIHNNIPNTNGTHCSVEPLDSSLCHNNIGMHLPFLNEFNEGGSQYPLAFSEDDLNTVVQMGFGQTSNRNTPIHSPSFNGNKTEKEKKKKKNNVTNY