Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023028.t1 | XP_013468829.1 | 92.5 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 1.00E-72 | 282.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023028.t1 | Q9FJK3 | 46.2 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 1.0e-28 | 127.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023028.t1 | A0A072VMQ1 | 92.5 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 7.5e-73 | 282.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene023028.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023028.t1 | MTR_1g077320 | 92.453 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 1.10e-97 | 278 |
| MS.gene023028.t1 | MTR_4g028720 | 87.421 | 159 | 20 | 0 | 1 | 159 | 1 | 159 | 2.45e-94 | 270 |
| MS.gene023028.t1 | MTR_7g011950 | 88.050 | 159 | 19 | 0 | 1 | 159 | 1 | 159 | 4.07e-93 | 267 |
| MS.gene023028.t1 | MTR_1g077390 | 71.975 | 157 | 44 | 0 | 1 | 157 | 1 | 157 | 3.67e-79 | 231 |
| MS.gene023028.t1 | MTR_1g077300 | 74.214 | 159 | 39 | 1 | 1 | 159 | 1 | 157 | 2.25e-74 | 219 |
| MS.gene023028.t1 | MTR_5g047580 | 67.949 | 156 | 50 | 0 | 1 | 156 | 1 | 156 | 2.85e-73 | 217 |
| MS.gene023028.t1 | MTR_1g084950 | 72.436 | 156 | 43 | 0 | 1 | 156 | 1 | 156 | 3.83e-72 | 214 |
| MS.gene023028.t1 | MTR_1g090697 | 56.129 | 155 | 68 | 0 | 1 | 155 | 1 | 155 | 6.91e-58 | 177 |
| MS.gene023028.t1 | MTR_1g090710 | 55.128 | 156 | 69 | 1 | 1 | 155 | 1 | 156 | 3.21e-56 | 174 |
| MS.gene023028.t1 | MTR_5g047560 | 57.051 | 156 | 51 | 1 | 1 | 156 | 1 | 140 | 2.85e-55 | 171 |
| MS.gene023028.t1 | MTR_1g090783 | 53.595 | 153 | 71 | 0 | 3 | 155 | 2 | 154 | 1.79e-54 | 169 |
| MS.gene023028.t1 | MTR_5g075380 | 39.744 | 156 | 93 | 1 | 1 | 155 | 1 | 156 | 8.97e-39 | 129 |
| MS.gene023028.t1 | MTR_3g065100 | 49.045 | 157 | 79 | 1 | 1 | 156 | 1 | 157 | 2.76e-38 | 130 |
| MS.gene023028.t1 | MTR_3g466980 | 43.137 | 153 | 86 | 1 | 1 | 152 | 1 | 153 | 1.29e-36 | 125 |
| MS.gene023028.t1 | MTR_4g063790 | 39.623 | 159 | 94 | 2 | 1 | 157 | 1 | 159 | 5.44e-35 | 120 |
| MS.gene023028.t1 | MTR_4g032620 | 36.129 | 155 | 97 | 2 | 1 | 153 | 1 | 155 | 6.66e-34 | 119 |
| MS.gene023028.t1 | MTR_3g466830 | 42.038 | 157 | 90 | 1 | 3 | 158 | 2 | 158 | 7.13e-34 | 117 |
| MS.gene023028.t1 | MTR_4g031910 | 43.333 | 150 | 83 | 2 | 1 | 148 | 1 | 150 | 2.80e-32 | 115 |
| MS.gene023028.t1 | MTR_3g466890 | 40.909 | 154 | 90 | 1 | 3 | 155 | 2 | 155 | 2.49e-31 | 110 |
| MS.gene023028.t1 | MTR_3g031240 | 35.849 | 159 | 100 | 2 | 1 | 157 | 1 | 159 | 4.27e-31 | 110 |
| MS.gene023028.t1 | MTR_2g016210 | 40.252 | 159 | 93 | 2 | 1 | 157 | 1 | 159 | 3.59e-30 | 110 |
| MS.gene023028.t1 | MTR_4g032260 | 42.500 | 160 | 90 | 2 | 1 | 158 | 1 | 160 | 4.57e-30 | 107 |
| MS.gene023028.t1 | MTR_8g036130 | 37.736 | 159 | 95 | 2 | 1 | 155 | 1 | 159 | 8.76e-30 | 108 |
| MS.gene023028.t1 | MTR_3g467080 | 33.962 | 159 | 103 | 2 | 1 | 157 | 1 | 159 | 1.14e-27 | 101 |
| MS.gene023028.t1 | MTR_4g032290 | 40.373 | 161 | 94 | 2 | 1 | 159 | 1 | 161 | 2.74e-27 | 102 |
| MS.gene023028.t1 | MTR_3g031100 | 36.076 | 158 | 97 | 3 | 1 | 155 | 1 | 157 | 2.81e-27 | 102 |
| MS.gene023028.t1 | MTR_2g035610 | 41.026 | 117 | 68 | 1 | 1 | 116 | 1 | 117 | 6.67e-24 | 90.5 |
| MS.gene023028.t1 | MTR_3g465410 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 1.17e-22 | 87.8 |
| MS.gene023028.t1 | MTR_2g035580 | 32.258 | 155 | 104 | 1 | 1 | 154 | 1 | 155 | 4.15e-21 | 84.3 |
| MS.gene023028.t1 | MTR_3g466930 | 36.800 | 125 | 78 | 1 | 32 | 155 | 2 | 126 | 1.35e-19 | 79.7 |
| MS.gene023028.t1 | MTR_3g466900 | 38.095 | 126 | 75 | 2 | 32 | 155 | 2 | 126 | 6.82e-19 | 77.8 |
| MS.gene023028.t1 | MTR_4g028800 | 45.205 | 73 | 38 | 1 | 1 | 73 | 1 | 71 | 8.24e-19 | 75.9 |
| MS.gene023028.t1 | MTR_4g019670 | 33.553 | 152 | 94 | 3 | 1 | 148 | 1 | 149 | 1.74e-18 | 81.6 |
| MS.gene023028.t1 | MTR_7g106510 | 31.544 | 149 | 98 | 2 | 1 | 148 | 4 | 149 | 5.32e-17 | 77.4 |
| MS.gene023028.t1 | MTR_3g093900 | 28.289 | 152 | 104 | 2 | 3 | 151 | 6 | 155 | 8.06e-14 | 68.2 |
| MS.gene023028.t1 | MTR_7g055800 | 32.456 | 114 | 50 | 2 | 1 | 114 | 1 | 87 | 1.73e-13 | 62.8 |
| MS.gene023028.t1 | MTR_7g055790 | 37.963 | 108 | 50 | 2 | 1 | 108 | 59 | 149 | 1.16e-12 | 62.0 |
| MS.gene023028.t1 | MTR_1g033790 | 39.583 | 96 | 57 | 1 | 64 | 158 | 22 | 117 | 2.22e-12 | 60.8 |
| MS.gene023028.t1 | MTR_1g033930 | 37.500 | 96 | 59 | 1 | 64 | 158 | 22 | 117 | 3.57e-12 | 60.1 |
| MS.gene023028.t1 | MTR_1g033370 | 39.362 | 94 | 56 | 1 | 64 | 156 | 28 | 121 | 2.04e-11 | 58.2 |
| MS.gene023028.t1 | MTR_7g055940 | 33.333 | 105 | 43 | 3 | 1 | 105 | 1 | 78 | 2.60e-11 | 56.6 |
| MS.gene023028.t1 | MTR_6g005450 | 29.464 | 112 | 73 | 3 | 1 | 106 | 1 | 112 | 6.93e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023028.t1 | AT5G48670 | 46.154 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 1.44e-34 | 123 |
| MS.gene023028.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 2.12e-30 | 109 |
| MS.gene023028.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 1.15e-29 | 108 |
| MS.gene023028.t1 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 1.69e-29 | 108 |
| MS.gene023028.t1 | AT5G26630 | 38.125 | 160 | 98 | 1 | 1 | 159 | 1 | 160 | 3.25e-28 | 104 |
| MS.gene023028.t1 | AT1G65300 | 36.054 | 147 | 93 | 1 | 3 | 148 | 2 | 148 | 2.31e-27 | 103 |
| MS.gene023028.t1 | AT1G65330 | 36.735 | 147 | 92 | 1 | 3 | 148 | 2 | 148 | 7.54e-27 | 102 |
| MS.gene023028.t1 | AT5G26650 | 35.294 | 153 | 98 | 1 | 4 | 155 | 2 | 154 | 2.14e-24 | 97.4 |
| MS.gene023028.t1 | AT5G27960 | 43.925 | 107 | 59 | 1 | 4 | 109 | 2 | 108 | 9.27e-24 | 94.7 |
| MS.gene023028.t1 | AT1G22590 | 29.630 | 162 | 98 | 3 | 1 | 155 | 1 | 153 | 3.75e-21 | 84.7 |
| MS.gene023028.t1 | AT5G27810 | 42.708 | 96 | 54 | 1 | 29 | 123 | 1 | 96 | 8.43e-20 | 80.1 |
| MS.gene023028.t1 | AT1G31630 | 29.032 | 155 | 108 | 2 | 3 | 156 | 2 | 155 | 1.76e-19 | 83.6 |
| MS.gene023028.t1 | AT2G28700 | 33.557 | 149 | 98 | 1 | 1 | 148 | 1 | 149 | 3.51e-19 | 82.8 |
| MS.gene023028.t1 | AT1G31640 | 38.739 | 111 | 66 | 2 | 3 | 112 | 2 | 111 | 3.38e-18 | 80.9 |
| MS.gene023028.t1 | AT5G26580 | 37.615 | 109 | 55 | 1 | 1 | 109 | 1 | 96 | 1.17e-16 | 75.9 |
| MS.gene023028.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 1.10e-13 | 67.8 |
| MS.gene023028.t1 | AT5G58890 | 34.234 | 111 | 65 | 3 | 1 | 104 | 1 | 110 | 5.76e-13 | 65.1 |
| MS.gene023028.t1 | AT5G06500 | 29.814 | 161 | 105 | 3 | 1 | 157 | 1 | 157 | 6.94e-13 | 64.3 |
Find 26 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAGAGGTGTGGCCAAATC+TGG | 0.333286 | 7.2:+49994122 | MS.gene023028:CDS |
| TGTTGAATCATTGGAGATAA+AGG | 0.341938 | 7.2:-49993986 | None:intergenic |
| CTTGTCTTTGCTTTCTCAGT+TGG | 0.347479 | 7.2:-49994254 | None:intergenic |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.353862 | 7.2:-49993995 | None:intergenic |
| GTTGCAAAAGGAAACTTTCC+TGG | 0.375490 | 7.2:-49994212 | None:intergenic |
| CTCCAATGATTCAACAAGAA+AGG | 0.407412 | 7.2:+49993993 | MS.gene023028:CDS |
| CAGAACTCATATATGAAAGA+TGG | 0.433670 | 7.2:+49994174 | MS.gene023028:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 7.2:-49994224 | None:intergenic |
| TGTTTCTGATTTGAATGACT+TGG | 0.473410 | 7.2:+49994350 | MS.gene023028:CDS |
| TCAGAAACAGTCATATCATC+TGG | 0.489973 | 7.2:-49994336 | None:intergenic |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.490416 | 7.2:-49993959 | None:intergenic |
| AGAGAACTCACTATTCTTTG+TGG | 0.499930 | 7.2:+49994057 | MS.gene023028:CDS |
| TGCAACAGATTACTAAAGCT+AGG | 0.531119 | 7.2:+49994229 | MS.gene023028:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.532673 | 7.2:+49994230 | MS.gene023028:CDS |
| AGAACTCATATATGAAAGAT+GGG | 0.538835 | 7.2:+49994175 | MS.gene023028:CDS |
| AGCTGAACATTCGTATGAGT+TGG | 0.547236 | 7.2:+49994292 | MS.gene023028:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.550366 | 7.2:+49994395 | MS.gene023028:CDS |
| CGAAGCCATGACTAGGAAGA+AGG | 0.551367 | 7.2:+49993954 | None:intergenic |
| GAAGAAAAGTATCATCAAGA+AGG | 0.556586 | 7.2:+49994032 | MS.gene023028:CDS |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 7.2:-49994088 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 7.2:+49994010 | MS.gene023028:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 7.2:-49994084 | None:intergenic |
| TACATTGCGAAGCCATGACT+AGG | 0.618148 | 7.2:+49993947 | None:intergenic |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 7.2:+49994112 | MS.gene023028:CDS |
| AGAGGTGTGGCCAAATCTGG+AGG | 0.647190 | 7.2:+49994125 | MS.gene023028:CDS |
| TTGGTACCTGCAAAACAACA+CGG | 0.687144 | 7.2:+49994311 | MS.gene023028:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAACTCATATATGAAAGAT+GGG | + | chr7.2:49994175-49994194 | MS.gene023028:CDS | 25.0% |
| !! | GTTAATTGCAAAAAGTATGA+AGG | + | chr7.2:49994377-49994396 | MS.gene023028:CDS | 25.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | - | chr7.2:49994106-49994125 | None:intergenic | 25.0% |
| CAGAACTCATATATGAAAGA+TGG | + | chr7.2:49994174-49994193 | MS.gene023028:CDS | 30.0% | |
| CTTTAGTAATCTGTTGCAAA+AGG | - | chr7.2:49994227-49994246 | None:intergenic | 30.0% | |
| TGTTGAATCATTGGAGATAA+AGG | - | chr7.2:49993989-49994008 | None:intergenic | 30.0% | |
| ! | TGTTTCTGATTTGAATGACT+TGG | + | chr7.2:49994350-49994369 | MS.gene023028:CDS | 30.0% |
| !! | GAAGAAAAGTATCATCAAGA+AGG | + | chr7.2:49994032-49994051 | MS.gene023028:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | - | chr7.2:49994111-49994130 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | + | chr7.2:49994107-49994126 | MS.gene023028:CDS | 30.0% |
| AGAGAACTCACTATTCTTTG+TGG | + | chr7.2:49994057-49994076 | MS.gene023028:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | - | chr7.2:49993998-49994017 | None:intergenic | 35.0% | |
| CTCCAATGATTCAACAAGAA+AGG | + | chr7.2:49993993-49994012 | MS.gene023028:CDS | 35.0% | |
| GAAAGATGGGAGAAAAAATG+TGG | + | chr7.2:49994188-49994207 | MS.gene023028:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | + | chr7.2:49994230-49994249 | MS.gene023028:CDS | 35.0% | |
| TCAGAAACAGTCATATCATC+TGG | - | chr7.2:49994339-49994358 | None:intergenic | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | + | chr7.2:49994229-49994248 | MS.gene023028:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | - | chr7.2:49994022-49994041 | None:intergenic | 35.0% |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | chr7.2:49994395-49994414 | MS.gene023028:CDS | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | + | chr7.2:49994112-49994131 | MS.gene023028:CDS | 35.0% |
| AGCTGAACATTCGTATGAGT+TGG | + | chr7.2:49994292-49994311 | MS.gene023028:CDS | 40.0% | |
| AGGATCGGAAATTATAGCAC+AGG | - | chr7.2:49994091-49994110 | None:intergenic | 40.0% | |
| CTTGTCTTTGCTTTCTCAGT+TGG | - | chr7.2:49994257-49994276 | None:intergenic | 40.0% | |
| GTTGCAAAAGGAAACTTTCC+TGG | - | chr7.2:49994215-49994234 | None:intergenic | 40.0% | |
| TTGGTACCTGCAAAACAACA+CGG | + | chr7.2:49994311-49994330 | MS.gene023028:CDS | 40.0% | |
| ! | TGTTTTGCTTCCTCCAGATT+TGG | - | chr7.2:49994138-49994157 | None:intergenic | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | + | chr7.2:49994010-49994029 | MS.gene023028:CDS | 45.0% | |
| TCGGAAATTATAGCACAGGC+TGG | - | chr7.2:49994087-49994106 | None:intergenic | 45.0% | |
| TGGGAGAAAAAATGTGGACC+AGG | + | chr7.2:49994194-49994213 | MS.gene023028:CDS | 45.0% | |
| ! | AACAGAGGTGTGGCCAAATC+TGG | + | chr7.2:49994122-49994141 | MS.gene023028:CDS | 50.0% |
| ! | AGAGGTGTGGCCAAATCTGG+AGG | + | chr7.2:49994125-49994144 | MS.gene023028:CDS | 55.0% |
| !!! | CTGGCACCGTGTTGTTTTGC+AGG | - | chr7.2:49994320-49994339 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 49993961 | 49994440 | 49993961 | ID=MS.gene023028 |
| chr7.2 | mRNA | 49993961 | 49994440 | 49993961 | ID=MS.gene023028.t1;Parent=MS.gene023028 |
| chr7.2 | exon | 49993961 | 49994440 | 49993961 | ID=MS.gene023028.t1.exon1;Parent=MS.gene023028.t1 |
| chr7.2 | CDS | 49993961 | 49994440 | 49993961 | ID=cds.MS.gene023028.t1;Parent=MS.gene023028.t1 |
| Gene Sequence |
| Protein sequence |
