AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023137

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023137.t1 MTR_4g094622 97.368 152 3 1 1 151 1 152 1.85e-104 295
MS.gene023137.t1 MTR_8g073345 46.067 89 48 0 59 147 33 121 1.05e-23 90.5
MS.gene023137.t1 MTR_8g073345 46.067 89 48 0 59 147 33 121 1.62e-23 89.7
MS.gene023137.t1 MTR_3g115580 47.674 86 45 0 53 138 35 120 3.08e-23 89.4
MS.gene023137.t1 MTR_7g068280 51.316 76 37 0 59 134 47 122 1.88e-22 87.8
MS.gene023137.t1 MTR_1g115220 48.315 89 45 1 59 146 98 186 1.09e-21 87.0
MS.gene023137.t1 MTR_7g082820 44.444 81 44 1 55 135 5 84 2.32e-17 73.9
MS.gene023137.t1 MTR_5g024180 38.272 81 50 0 52 132 25 105 4.54e-15 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023137.t1 AT2G34450 55.921 152 65 2 1 151 1 151 4.92e-51 160
MS.gene023137.t1 AT2G34450 55.921 152 65 2 1 151 1 151 4.92e-51 160
MS.gene023137.t1 AT2G34450 55.921 152 65 2 1 151 1 151 4.92e-51 160
MS.gene023137.t1 AT2G34450 55.556 153 65 3 1 151 1 152 3.30e-49 155
MS.gene023137.t1 AT2G17560 43.478 115 61 1 41 151 13 127 8.43e-24 90.5
MS.gene023137.t1 AT2G17560 43.478 115 61 1 41 151 13 127 8.97e-24 90.5
MS.gene023137.t1 AT2G17560 43.478 115 61 1 41 151 13 127 8.97e-24 90.5
MS.gene023137.t1 AT3G51880 44.554 101 56 0 50 150 40 140 1.60e-23 90.5
MS.gene023137.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.06e-23 90.5
MS.gene023137.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.06e-23 90.5
MS.gene023137.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.06e-23 90.5
MS.gene023137.t1 AT3G51880 44.118 102 57 0 50 151 40 141 2.26e-23 90.9
MS.gene023137.t1 AT1G20693 46.667 90 46 1 59 148 34 121 5.03e-22 86.3
MS.gene023137.t1 AT1G20693 46.667 90 46 1 59 148 34 121 5.16e-22 86.3
MS.gene023137.t1 AT4G35570 44.318 88 49 0 59 146 30 117 5.58e-22 85.5
MS.gene023137.t1 AT4G35570 44.318 88 49 0 59 146 30 117 5.58e-22 85.5
MS.gene023137.t1 AT1G20693 46.667 90 46 1 59 148 34 121 1.19e-21 85.1
MS.gene023137.t1 AT1G20696 45.556 90 47 1 59 148 31 118 7.78e-21 82.8
MS.gene023137.t1 AT1G20696 44.156 77 43 0 59 135 31 107 4.96e-18 75.9
MS.gene023137.t1 AT1G20696 44.156 77 43 0 59 135 31 107 5.22e-18 75.5
MS.gene023137.t1 AT5G23420 47.059 68 35 1 60 127 112 178 6.33e-13 64.3
MS.gene023137.t1 AT1G20696 43.243 74 40 1 75 148 5 76 4.62e-12 59.3
MS.gene023137.t1 AT1G20696 43.243 74 40 1 75 148 5 76 1.25e-11 58.2

Find 21 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATTCTGCACGTTTCT+CGG 0.263566 4.3:+19053147 None:intergenic
TCTTCTTCGGCCTCCACCTC+CGG 0.314496 4.3:-19055901 MS.gene023137:CDS
GACAGAGTATAACAAGAAAA+TGG 0.358050 4.3:-19053116 MS.gene023137:intron
TTAAGTCTCTTGACTTAGTC+TGG 0.383960 4.3:+19055279 None:intergenic
TCAAAAGAACGCTTCTTCTT+CGG 0.408803 4.3:-19055914 MS.gene023137:CDS
TCACGCATTGACTTAACATC+TGG 0.420177 4.3:+19054496 None:intergenic
CTTCTTCGGCCTCCACCTCC+GGG 0.468963 4.3:-19055900 MS.gene023137:intron
AGTGAAAGGGTACCCGGAGG+TGG 0.487598 4.3:+19055888 None:intergenic
GAAAGGGTACCCGGAGGTGG+AGG 0.521126 4.3:+19055891 None:intergenic
GCAAGGCATGTGGAGAGAAG+TGG 0.571530 4.3:-19054199 MS.gene023137:CDS
CGTGTTCATACAGATTGGCA+AGG 0.572075 4.3:-19054216 MS.gene023137:intron
AAGAAGTAGAAGAAAGCAGT+GGG 0.576065 4.3:+19055197 None:intergenic
GTTGGCGATCAAATCGAATG+AGG 0.587280 4.3:-19055321 MS.gene023137:CDS
GAAGTAGAAGAAAGCAGTGG+GGG 0.593069 4.3:+19055199 None:intergenic
TCTGATGCTTTGCAGGAAAG+CGG 0.595152 4.3:-19053021 MS.gene023137:intron
ATACAGATTGGCAAGGCATG+TGG 0.602187 4.3:-19054209 MS.gene023137:intron
CGAATATGAAGAAACTGATG+AGG 0.603289 4.3:-19052998 MS.gene023137:CDS
TTGGCGATCAAATCGAATGA+GGG 0.633911 4.3:-19055320 MS.gene023137:CDS
CAAGAAGTAGAAGAAAGCAG+TGG 0.644048 4.3:+19055196 None:intergenic
AAGTAGAAGAAAGCAGTGGG+GGG 0.691336 4.3:+19055200 None:intergenic
AGAAGTAGAAGAAAGCAGTG+GGG 0.730489 4.3:+19055198 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAAGTTTCAAAAAT+TGG - chr4.3:19054854-19054873 MS.gene023137:intron 10.0%
!! ATAAATTATGTTTCAAAAAT+TGG - chr4.3:19054929-19054948 MS.gene023137:intron 10.0%
!! TAAAAAAAATTAAAGTAAAG+GGG - chr4.3:19055449-19055468 MS.gene023137:intron 10.0%
!! TTGTTATATTTGAAAAAAAT+TGG - chr4.3:19055086-19055105 MS.gene023137:intron 10.0%
!! TCATTCAAGTAAAAAATTAT+TGG - chr4.3:19053337-19053356 MS.gene023137:intron 15.0%
!!! CGATTTTTATGTTTAATTAT+TGG - chr4.3:19055291-19055310 MS.gene023137:CDS 15.0%
!! AAAATTGAATAAAATGATGG+CGG - chr4.3:19053430-19053449 MS.gene023137:intron 20.0%
!! AACATCATAAATAAAGAACA+AGG + chr4.3:19053770-19053789 None:intergenic 20.0%
!! AATAAGCAGAAAACAATTTA+TGG + chr4.3:19053153-19053172 None:intergenic 20.0%
!! ACTACTATTTACAGTATATT+TGG - chr4.3:19054245-19054264 MS.gene023137:intron 20.0%
!! AGATCAAATTGAACATAATA+TGG + chr4.3:19055666-19055685 None:intergenic 20.0%
!! TCTCAAAAACATAAAATAAC+TGG + chr4.3:19054601-19054620 None:intergenic 20.0%
!! TGTAAAAATTAGAAGATTCA+GGG - chr4.3:19053311-19053330 MS.gene023137:intron 20.0%
!! TTATGTTTAATTATTGGTGT+TGG - chr4.3:19055297-19055316 MS.gene023137:CDS 20.0%
!! TTGTAAAAATTAGAAGATTC+AGG - chr4.3:19053310-19053329 MS.gene023137:intron 20.0%
!! TTTGAAAAAAATTGGAAAGT+TGG - chr4.3:19055094-19055113 MS.gene023137:intron 20.0%
!!! AAACTAGTTCTATATTGATT+TGG + chr4.3:19055595-19055614 None:intergenic 20.0%
!!! AAATTGATTTTTAGACCTAT+AGG - chr4.3:19053266-19053285 MS.gene023137:intron 20.0%
!!! AATTGATTTTTAGACCTATA+GGG - chr4.3:19053267-19053286 MS.gene023137:intron 20.0%
!!! AATTTAGAACAAATGGAAAA+TGG + chr4.3:19053832-19053851 None:intergenic 20.0%
!!! ACAATTGCTTCTTTTTTATA+TGG - chr4.3:19054309-19054328 MS.gene023137:intron 20.0%
!!! ATAACTGGCAATTTTTAAAA+TGG + chr4.3:19054586-19054605 None:intergenic 20.0%
!!! CCATAAAAATTTAGAACAAA+TGG + chr4.3:19053839-19053858 None:intergenic 20.0%
!!! CCATCATTTTATTCAATTTT+GGG + chr4.3:19053430-19053449 None:intergenic 20.0%
!!! CCATTTGTTCTAAATTTTTA+TGG - chr4.3:19053836-19053855 MS.gene023137:intron 20.0%
! AATAAGACATAGTAGAAACA+AGG + chr4.3:19054208-19054227 None:intergenic 25.0%
! ATCATAAATAAAGAACAAGG+AGG + chr4.3:19053767-19053786 None:intergenic 25.0%
! ATTATAAAGACACGTAAACT+GGG + chr4.3:19055413-19055432 None:intergenic 25.0%
! CCCAAAATTGAATAAAATGA+TGG - chr4.3:19053427-19053446 MS.gene023137:intron 25.0%
! CTAACTTTCTTCTATAGTTA+CGG - chr4.3:19053103-19053122 MS.gene023137:intron 25.0%
! TAACAAAAATTGTATCTGAC+AGG - chr4.3:19055724-19055743 MS.gene023137:intron 25.0%
! TAGACGAAATATTAATACGA+CGG + chr4.3:19054465-19054484 None:intergenic 25.0%
! TAGTAATGAACCAGTAATTA+TGG + chr4.3:19053933-19053952 None:intergenic 25.0%
! TTCAAAATTACTGTGTTTAC+TGG - chr4.3:19054347-19054366 MS.gene023137:intron 25.0%
!! AATTCAGGTTAAATATGCAA+GGG + chr4.3:19054788-19054807 None:intergenic 25.0%
!! GAATTTTGTCAAAACTGTTT+AGG - chr4.3:19054958-19054977 MS.gene023137:intron 25.0%
!! GCCATCATTTTATTCAATTT+TGG + chr4.3:19053431-19053450 None:intergenic 25.0%
!! TAACCTGAATTGTGTTATTA+TGG - chr4.3:19054797-19054816 MS.gene023137:intron 25.0%
!!! AACTGTTTAGGTTTTTAATG+TGG - chr4.3:19054970-19054989 MS.gene023137:intron 25.0%
!!! AAGTTCTTTTTCATTCTGAA+GGG - chr4.3:19054370-19054389 MS.gene023137:intron 25.0%
!!! ATCAGTTTTTTGACTCATTT+AGG - chr4.3:19055249-19055268 MS.gene023137:CDS 25.0%
!!! TCAGTTTTTTGACTCATTTA+GGG - chr4.3:19055250-19055269 MS.gene023137:CDS 25.0%
!!! TTATTCAATTTTGGGTCTAA+GGG + chr4.3:19053422-19053441 None:intergenic 25.0%
!!! TTTAATTTTCAGAAGCTCTT+CGG - chr4.3:19053240-19053259 MS.gene023137:intron 25.0%
!!! TTTATTCAATTTTGGGTCTA+AGG + chr4.3:19053423-19053442 None:intergenic 25.0%
AAAACATGTTTGAAGATGAG+TGG + chr4.3:19054141-19054160 None:intergenic 30.0%
AAAAGTAGCACCATAATTAC+TGG - chr4.3:19053920-19053939 MS.gene023137:intron 30.0%
ATAGTAGTATGATTGAGCAA+AGG + chr4.3:19054233-19054252 None:intergenic 30.0%
ATTAACACGAGTACATTTGT+AGG - chr4.3:19055324-19055343 MS.gene023137:CDS 30.0%
CAACCATAATAACACAATTC+AGG + chr4.3:19054803-19054822 None:intergenic 30.0%
GACAGAGTATAACAAGAAAA+TGG - chr4.3:19055805-19055824 MS.gene023137:intron 30.0%
GATTATAAAGACACGTAAAC+TGG + chr4.3:19055414-19055433 None:intergenic 30.0%
GCTAATTGCTAATCAACAAA+TGG + chr4.3:19054087-19054106 None:intergenic 30.0%
TAACCTATCTGTCATATTCA+TGG - chr4.3:19055828-19055847 MS.gene023137:intron 30.0%
TAGACCTATAGGGATATTAA+TGG - chr4.3:19053277-19053296 MS.gene023137:intron 30.0%
TGTAAAACAAAGTTGCTTCA+GGG + chr4.3:19054511-19054530 None:intergenic 30.0%
TTACAGTATATTTGGCATTG+TGG - chr4.3:19054253-19054272 MS.gene023137:intron 30.0%
TTGTAAAACAAAGTTGCTTC+AGG + chr4.3:19054512-19054531 None:intergenic 30.0%
! AACAATGATGATGATGATGA+TGG + chr4.3:19054636-19054655 None:intergenic 30.0%
! ATGTTTCAAAAATTGGCTCA+GGG - chr4.3:19054936-19054955 MS.gene023137:intron 30.0%
! ATTTGGAATAAATAGCAGCA+CGG + chr4.3:19055578-19055597 None:intergenic 30.0%
! CAATTCAGGTTAAATATGCA+AGG + chr4.3:19054789-19054808 None:intergenic 30.0%
! TATGTTTCAAAAATTGGCTC+AGG - chr4.3:19054935-19054954 MS.gene023137:intron 30.0%
! TTAATTTTCAGAAGCTCTTC+GGG - chr4.3:19053241-19053260 MS.gene023137:intron 30.0%
!! GAAGTTCTTTTTCATTCTGA+AGG - chr4.3:19054369-19054388 MS.gene023137:intron 30.0%
!! TGGATGGTATTTTAGAAAAC+TGG + chr4.3:19055482-19055501 None:intergenic 30.0%
!! TTCTAAAATACCATCCATTG+TGG - chr4.3:19055485-19055504 MS.gene023137:intron 30.0%
!!! GTATTTTACGGATTTTGACT+TGG - chr4.3:19054039-19054058 MS.gene023137:intron 30.0%
AAGAAGTAGAAGAAAGCAGT+GGG + chr4.3:19053727-19053746 None:intergenic 35.0%
AAGAAGTCATGATCAAGACA+TGG + chr4.3:19055017-19055036 None:intergenic 35.0%
ACAAGAGTTTAGCTGCATTA+AGG - chr4.3:19053876-19053895 MS.gene023137:intron 35.0%
ATCAAATTCTGCACGTTTCT+CGG + chr4.3:19055777-19055796 None:intergenic 35.0%
CACTCCATTAATATCCCTAT+AGG + chr4.3:19053284-19053303 None:intergenic 35.0%
CCATTGTCAGGAATTTAAAC+AGG + chr4.3:19055631-19055650 None:intergenic 35.0%
CCTGTTTAAATTCCTGACAA+TGG - chr4.3:19055628-19055647 MS.gene023137:intron 35.0%
CGAATATGAAGAAACTGATG+AGG - chr4.3:19055923-19055942 MS.gene023137:CDS 35.0%
GACATAGTAGAAACAAGGAA+AGG + chr4.3:19054203-19054222 None:intergenic 35.0%
GTAAAACAAAGTTGCTTCAG+GGG + chr4.3:19054510-19054529 None:intergenic 35.0%
TAGCCATGAATATGACAGAT+AGG + chr4.3:19055834-19055853 None:intergenic 35.0%
TCAAAAGAACGCTTCTTCTT+CGG - chr4.3:19053007-19053026 MS.gene023137:CDS 35.0%
TCAATTTGATCTGAGCAAGA+AGG - chr4.3:19055674-19055693 MS.gene023137:intron 35.0%
TGGAAAGTTGGAATTCATTG+CGG - chr4.3:19055106-19055125 MS.gene023137:intron 35.0%
TTAAGTCTCTTGACTTAGTC+TGG + chr4.3:19053645-19053664 None:intergenic 35.0%
TTATGACCAATGCGCAATTT+AGG + chr4.3:19053367-19053386 None:intergenic 35.0%
TTGTCAGTTGTGATGTTTGA+CGG - chr4.3:19054888-19054907 MS.gene023137:intron 35.0%
TTTCTTCTATAGTTACGGTC+TGG - chr4.3:19053108-19053127 MS.gene023137:intron 35.0%
! AATGCTGCATTGAGTTTTTC+AGG - chr4.3:19053569-19053588 MS.gene023137:intron 35.0%
! CTAGTGAACTGACTCTTATT+TGG - chr4.3:19055851-19055870 MS.gene023137:intron 35.0%
! GCGCATTGGTCATAATAAAA+AGG - chr4.3:19053372-19053391 MS.gene023137:intron 35.0%
! GTGGAAAACAATGACTTTTG+AGG - chr4.3:19054741-19054760 MS.gene023137:intron 35.0%
! TATACTATCGCGTAAGTTGA+TGG - chr4.3:19054006-19054025 MS.gene023137:intron 35.0%
!! ATACTATCGCGTAAGTTGAT+GGG - chr4.3:19054007-19054026 MS.gene023137:intron 35.0%
!! CTTATTTTCACAAGCTCTTC+AGG - chr4.3:19053167-19053186 MS.gene023137:CDS 35.0%
!! TTCTTTTTCATTCTGAAGGG+AGG - chr4.3:19054373-19054392 MS.gene023137:intron 35.0%
!! TTTGTTTTCTGATGCTTTGC+AGG - chr4.3:19055893-19055912 MS.gene023137:intron 35.0%
!!! GTTCTTTTTTTGCACTGCTT+TGG + chr4.3:19053668-19053687 None:intergenic 35.0%
!!! GTTTTTCAGGAAACTAGTGT+TGG - chr4.3:19053582-19053601 MS.gene023137:intron 35.0%
!!! TTTTACGGATTTTGACTTGG+AGG - chr4.3:19054042-19054061 MS.gene023137:intron 35.0%
AACATAGAGCTCAACCAAAG+CGG + chr4.3:19055705-19055724 None:intergenic 40.0%
ACGCGATAGTATAACTCCAA+AGG + chr4.3:19053999-19054018 None:intergenic 40.0%
ACTGATCTGTGTGTTTCCTT+TGG - chr4.3:19053980-19053999 MS.gene023137:intron 40.0%
AGAAGTAGAAGAAAGCAGTG+GGG + chr4.3:19053726-19053745 None:intergenic 40.0%
AGCAGAACTGAAGATCACTT+TGG - chr4.3:19055131-19055150 MS.gene023137:intron 40.0%
AGGAGAGTAGAGAAAGTGAA+AGG + chr4.3:19053050-19053069 None:intergenic 40.0%
AGGTAAGTTTCCACAATGGA+TGG + chr4.3:19055498-19055517 None:intergenic 40.0%
ATACTTGACACTCCATTGTC+AGG + chr4.3:19055643-19055662 None:intergenic 40.0%
ATCCATTGTGGAAACTTACC+TGG - chr4.3:19055497-19055516 MS.gene023137:intron 40.0%
CAAGAAGTAGAAGAAAGCAG+TGG + chr4.3:19053728-19053747 None:intergenic 40.0%
CAATCTGTATGAACACGGAA+AGG + chr4.3:19054702-19054721 None:intergenic 40.0%
CCAACAATGCAATTATGCCT+TGG - chr4.3:19053396-19053415 MS.gene023137:intron 40.0%
CCAAGGCATAATTGCATTGT+TGG + chr4.3:19053399-19053418 None:intergenic 40.0%
CTTGCCAATCTGTATGAACA+CGG + chr4.3:19054707-19054726 None:intergenic 40.0%
CTTTCCGTGTTCATACAGAT+TGG - chr4.3:19054700-19054719 MS.gene023137:intron 40.0%
GGAGAGTAGAGAAAGTGAAA+GGG + chr4.3:19053049-19053068 None:intergenic 40.0%
TAGCTTCAGGTGTTAGTAGT+GGG - chr4.3:19053489-19053508 MS.gene023137:intron 40.0%
TCCATTGTGGAAACTTACCT+GGG - chr4.3:19055498-19055517 MS.gene023137:intron 40.0%
TTGGCGATCAAATCGAATGA+GGG - chr4.3:19053601-19053620 MS.gene023137:intron 40.0%
! ACTCTCCTTTTCGTTCACAA+GGG - chr4.3:19053062-19053081 MS.gene023137:intron 40.0%
! GGGGGTTCTGTTGTATTTTA+CGG - chr4.3:19054027-19054046 MS.gene023137:intron 40.0%
! TACTCTCCTTTTCGTTCACA+AGG - chr4.3:19053061-19053080 MS.gene023137:intron 40.0%
!! CATTCTGAAGGGAGGATTTT+CGG - chr4.3:19054381-19054400 MS.gene023137:intron 40.0%
!! TACTATCGCGTAAGTTGATG+GGG - chr4.3:19054008-19054027 MS.gene023137:intron 40.0%
!! TCACGCATTGACTTAACATC+TGG + chr4.3:19054428-19054447 None:intergenic 40.0%
AAGTAGAAGAAAGCAGTGGG+GGG + chr4.3:19053724-19053743 None:intergenic 45.0%
AGCTTCAGGTGTTAGTAGTG+GGG - chr4.3:19053490-19053509 MS.gene023137:intron 45.0%
ATACAGATTGGCAAGGCATG+TGG - chr4.3:19054712-19054731 MS.gene023137:intron 45.0%
CCTTTCCCTTGTGAACGAAA+AGG + chr4.3:19053070-19053089 None:intergenic 45.0%
CGTGTTCATACAGATTGGCA+AGG - chr4.3:19054705-19054724 MS.gene023137:intron 45.0%
CTACTAACACCTGAAGCTAG+AGG + chr4.3:19053488-19053507 None:intergenic 45.0%
CTAGCTTCAGGTGTTAGTAG+TGG - chr4.3:19053488-19053507 MS.gene023137:intron 45.0%
GAAGTAGAAGAAAGCAGTGG+GGG + chr4.3:19053725-19053744 None:intergenic 45.0%
GGAAGACCTAAATTGCGCAT+TGG - chr4.3:19053358-19053377 MS.gene023137:intron 45.0%
GTCTCAGCTCTTGAACTTGA+GGG + chr4.3:19054116-19054135 None:intergenic 45.0%
GTTGGCGATCAAATCGAATG+AGG - chr4.3:19053600-19053619 MS.gene023137:intron 45.0%
TCCCAGGTAAGTTTCCACAA+TGG + chr4.3:19055502-19055521 None:intergenic 45.0%
TCTCAGCTCTTGAACTTGAG+GGG + chr4.3:19054115-19054134 None:intergenic 45.0%
TCTGATGCTTTGCAGGAAAG+CGG - chr4.3:19055900-19055919 MS.gene023137:intron 45.0%
TCTGTATGAACACGGAAAGG+AGG + chr4.3:19054699-19054718 None:intergenic 45.0%
TGGAAACTTACCTGGGACTA+AGG - chr4.3:19055505-19055524 MS.gene023137:intron 45.0%
TGTCTCAGCTCTTGAACTTG+AGG + chr4.3:19054117-19054136 None:intergenic 45.0%
TGTGAGTTTCAACCGTGTCT+AGG - chr4.3:19053456-19053475 MS.gene023137:intron 45.0%
! CCTTTTCGTTCACAAGGGAA+AGG - chr4.3:19053067-19053086 MS.gene023137:intron 45.0%
! TAGAGGTGTAGACCTAGACA+CGG + chr4.3:19053471-19053490 None:intergenic 45.0%
!! AATTTTGGGTCTAAGGGCCA+AGG + chr4.3:19053416-19053435 None:intergenic 45.0%
!! ACTATCGCGTAAGTTGATGG+GGG - chr4.3:19054009-19054028 MS.gene023137:intron 45.0%
!! AGAGAAAGTGAAAGGGTACC+CGG + chr4.3:19053042-19053061 None:intergenic 45.0%
!! AGCGTTCTTTTGAGAGTGAG+AGG + chr4.3:19053000-19053019 None:intergenic 45.0%
!!! CGGATTTTGACTTGGAGGAA+TGG - chr4.3:19054047-19054066 MS.gene023137:intron 45.0%
!! AATAAAAAAAATTAAAGTAA+AGG - chr4.3:19055447-19055466 MS.gene023137:intron 5.0%
!! ATAAAAAAAATTAAAGTAAA+GGG - chr4.3:19055448-19055467 MS.gene023137:intron 5.0%
AAAGCAGTGGGGGGTTTCTT+CGG + chr4.3:19053715-19053734 None:intergenic 50.0%
AACTGATGATCATGCCAGCC+TGG + chr4.3:19055236-19055255 None:intergenic 50.0%
AGCTTCTTCTCGATAGCAGC+AGG + chr4.3:19055541-19055560 None:intergenic 50.0%
CACGTAAACTGGGTGCTCTA+AGG + chr4.3:19055403-19055422 None:intergenic 50.0%
CTCAGCTCTTGAACTTGAGG+GGG + chr4.3:19054114-19054133 None:intergenic 50.0%
GCAAGAAGGAAACACCGCTT+TGG - chr4.3:19055688-19055707 MS.gene023137:intron 50.0%
TCTCCTCTGGTTGGAATTCC+AGG - chr4.3:19055215-19055234 MS.gene023137:CDS 50.0%
! AGGTCTACACCTCTAGCTTC+AGG - chr4.3:19053476-19053495 MS.gene023137:intron 50.0%
AGTCGCAGAACTGTCTCCTC+TGG - chr4.3:19055202-19055221 MS.gene023137:CDS 55.0%
CAGCCTGGAATTCCAACCAG+AGG + chr4.3:19055221-19055240 None:intergenic 55.0%
CTCTGGTTGGAATTCCAGGC+TGG - chr4.3:19055219-19055238 MS.gene023137:CDS 55.0%
CTTTCACGTGCCTTAGTCCC+AGG + chr4.3:19055518-19055537 None:intergenic 55.0%
GACTTGGAGGAATGGTGCAG+AGG - chr4.3:19054055-19054074 MS.gene023137:intron 55.0%
GCAAGGCATGTGGAGAGAAG+TGG - chr4.3:19054722-19054741 MS.gene023137:intron 55.0%
GCAGAACTGTCTCCTCTGGT+TGG - chr4.3:19055206-19055225 MS.gene023137:CDS 55.0%
TTGGAGGAATGGTGCAGAGG+AGG - chr4.3:19054058-19054077 MS.gene023137:intron 55.0%
!! GAAAGTGAAAGGGTACCCGG+AGG + chr4.3:19053039-19053058 None:intergenic 55.0%
TCTTCTTCGGCCTCCACCTC+CGG - chr4.3:19053020-19053039 MS.gene023137:intron 60.0%
!! AGTGAAAGGGTACCCGGAGG+TGG + chr4.3:19053036-19053055 None:intergenic 60.0%
CTTCTTCGGCCTCCACCTCC+GGG - chr4.3:19053021-19053040 MS.gene023137:intron 65.0%
!! GAAAGGGTACCCGGAGGTGG+AGG + chr4.3:19053033-19053052 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 19052978 19055965 19052978 ID=MS.gene023137
chr4.3 mRNA 19052978 19055965 19052978 ID=MS.gene023137.t1;Parent=MS.gene023137
chr4.3 exon 19055901 19055965 19055901 ID=MS.gene023137.t1.exon1;Parent=MS.gene023137.t1
chr4.3 CDS 19055901 19055965 19055901 ID=cds.MS.gene023137.t1;Parent=MS.gene023137.t1
chr4.3 exon 19055197 19055352 19055197 ID=MS.gene023137.t1.exon2;Parent=MS.gene023137.t1
chr4.3 CDS 19055197 19055352 19055197 ID=cds.MS.gene023137.t1;Parent=MS.gene023137.t1
chr4.3 exon 19054495 19054552 19054495 ID=MS.gene023137.t1.exon3;Parent=MS.gene023137.t1
chr4.3 CDS 19054495 19054552 19054495 ID=cds.MS.gene023137.t1;Parent=MS.gene023137.t1
chr4.3 exon 19054181 19054225 19054181 ID=MS.gene023137.t1.exon4;Parent=MS.gene023137.t1
chr4.3 CDS 19054181 19054225 19054181 ID=cds.MS.gene023137.t1;Parent=MS.gene023137.t1
chr4.3 exon 19053117 19053197 19053117 ID=MS.gene023137.t1.exon5;Parent=MS.gene023137.t1
chr4.3 CDS 19053117 19053197 19053117 ID=cds.MS.gene023137.t1;Parent=MS.gene023137.t1
chr4.3 exon 19052978 19053028 19052978 ID=MS.gene023137.t1.exon6;Parent=MS.gene023137.t1
chr4.3 CDS 19052978 19053028 19052978 ID=cds.MS.gene023137.t1;Parent=MS.gene023137.t1
Gene Sequence

>MS.gene023137

ATGGCGAAAAAAGCTAAAGCCTCTCACTCTCAAAAGAACGCTTCTTCTTCGGCCTCCACCTCCGGGTACCCTTTCACTTTCTCTACTCTCCTTTTCGTTCACAAGGGAAAGGCTATATTTTAAAACTAACTTTCTTCTATAGTTACGGTCTGGAGACAATTTATGACATCGTCCATAAATTGTTTTCTGCTTATTTTCACAAGCTCTTCAGGATAACTTATGAAAACAGCTTGCAGCTTACATGAAAACAAATTGATTTTATTTTAATTTTCAGAAGCTCTTCGGGATAAATTGATTTTTAGACCTATAGGGATATTAATGGAGTGATATGATTGTAAAAATTAGAAGATTCAGGGACTTCATTCAAGTAAAAAATTATTGGAAGACCTAAATTGCGCATTGGTCATAATAAAAAGGACCAACAATGCAATTATGCCTTGGCCCTTAGACCCAAAATTGAATAAAATGATGGCGGCATTGTGAGTTTCAACCGTGTCTAGGTCTACACCTCTAGCTTCAGGTGTTAGTAGTGGGGAAATTTGATTTATTACATTTAACTATGTGAAGAAATAGTTTTGCTTAACTAAACACAATGCTGCATTGAGTTTTTCAGGAAACTAGTGTTGGCGATCAAATCGAATGAGGGAATGAAGAGATCGTTGCGCCAGACTAAGTCAAGAGACTTAACCAAAGCAGTGCAAAAAAAGAACAAGCAAAAGATTGATGCTAAGAAGCCGAAGAAACCCCCCACTGCTTTCTTCTACTTCTTGTAAAACTTCTTTTATTCCTCCTTGTTCTTTATTTATGATGTTTAATTCTTATTTTTAATGTGTTAGTATAAATTACTCTTTCCATTTTCCATTTGTTCTAAATTTTTATGGAATATATTATTAATTTAACAAGAGTTTAGCTGCATTAAGGTTTATTTGTCTGTATATATGAAAAAGTAGCACCATAATTACTGGTTCATTACTAGAATGAACAAGTATAGTTAATGTTCTTACTGATCTGTGTGTTTCCTTTGGAGTTATACTATCGCGTAAGTTGATGGGGGTTCTGTTGTATTTTACGGATTTTGACTTGGAGGAATGGTGCAGAGGAGGTAACCATTTGTTGATTAGCAATTAGCATTACCCCCTCAAGTTCAAGAGCTGAGACAACCACTCATCTTCAAACATGTTTTATCATTAGTCATTTAAAAACAATTTTCTTCTGATAGTTTCCTTTCCTTGTTTCTACTATGTCTTATTGTCCTTTGCTCAATCATACTACTATTTACAGTATATTTGGCATTGTGGTAATTAATCTAGCACAGTGACTGTTTGTAACAAACAATTGCTTCTTTTTTATATGGAAAATCTTACAATGCTTCAAAATTACTGTGTTTACTGGAAGTTCTTTTTCATTCTGAAGGGAGGATTTTCGGAAAGAGTTTAAAGATCAGAATCCAGATGTTAAGTCAATGCGTGATGTATGTATAGAATCCGTCGTATTAATATTTCGTCTAATTTATTTTATACTTTTAATTTCCCCTGAAGCAACTTTGTTTTACAATTGATCGTTTAGAATTTTCAAAATTATATAAACAACACAGATGTCTAACATCCATTTTAAAAATTGCCAGTTATTTTATGTTTTTGAGACTGAAAATATCACCATCATCATCATCATCATTGTTTATCATCATCATGCAAAGTAATATTTTTCTGTTTTGTTTTCCTCCTTTCCGTGTTCATACAGATTGGCAAGGCATGTGGAGAGAAGTGGAAAACAATGACTTTTGAGGTTAGTTTTTCTTTGCTCTTCTCCCTTGCATATTTAACCTGAATTGTGTTATTATGGTTGTGATGTTTGACTGCTGAAAACTGAGTGAATCATAAATTAAGTTTCAAAAATTGGCTCAGAGAATTTTGTCAGTTGTGATGTTTGACGGCTAAAAACTGAGTGAATCATAAATTATGTTTCAAAAATTGGCTCAGGGAATTTTGTCAAAACTGTTTAGGTTTTTAATGTGGCTGAATGTTTGAACTTGATTGCCATGTCTTGATCATGACTTCTTCGAATATCAATGAATTAAATTTTGAACAAAATTCTTGATGAACAAGTTTTTGTTATATTTGAAAAAAATTGGAAAGTTGGAATTCATTGCGGCAAGCAGAACTGAAGATCACTTTGGCATTTCTACGTTATGTACAATTTTGCAATTCAGCTACTGTAATGCAAAAGTCGCAGAACTGTCTCCTCTGGTTGGAATTCCAGGCTGGCATGATCATCAGTTTTTTGACTCATTTAGGGACAGAGTGTTTTTTTCTTCGATTTTTATGTTTAATTATTGGTGTTGGTAGCATTAACACGAGTACATTTGTAGGTTTGTAGTACTTGTATTGACTGCACAAAATGTTGATTCAGTCATTTCATCAAACCTTAGAGCACCCAGTTTACGTGTCTTTATAATCAATCATTTTTAAAAATAAAAAAAATTAAAGTAAAGGGGTTCAAAACCAGTTTTCTAAAATACCATCCATTGTGGAAACTTACCTGGGACTAAGGCACGTGAAAGCCTGCTGCTATCGAGAAGAAGCTTCTGCTTATGCTAACCGTGCTGCTATTTATTCCAAATCAATATAGAACTAGTTTTTCAAGTTTGTTTCCTGTTTAAATTCCTGACAATGGAGTGTCAAGTATCCATATTATGTTCAATTTGATCTGAGCAAGAAGGAAACACCGCTTTGGTTGAGCTCTATGTTAACAAAAATTGTATCTGACAGGAGAAAGTTCAATACTATGATATAGCAACCGAGAAACGTGCAGAATTTGATAGAGCAACGACAGAGTATAACAAGAAAATGGTAACCTATCTGTCATATTCATGGCTAGTGAACTGACTCTTATTTGGCATGTTATTAAATATACAATTTGTTTTCTGATGCTTTGCAGGAAAGCGGCGAATATGAAGAAACTGATGAGGAGTCAGAGTATGACGAGTAA
Protein sequence

>MS.gene023137.t1

MAKKAKASHSQKNASSSASTSGKLVLAIKSNEGMKRSLRQTKSRDLTKAVQKKNKQKIDAKKPKKPPTAFFYFLEDFRKEFKDQNPDVKSMRDIGKACGEKWKTMTFEEKVQYYDIATEKRAEFDRATTEYNKKMESGEYEETDEESEYDE