AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023309

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023309.t1 MTR_3g065980 62.956 548 146 12 13 516 7 541 0.0 667
MS.gene023309.t1 MTR_7g027190 40.093 429 213 11 120 515 256 673 6.26e-95 303
MS.gene023309.t1 MTR_8g442410 35.825 388 218 9 155 518 148 528 2.69e-76 250
MS.gene023309.t1 MTR_5g097480 36.702 376 214 8 159 515 174 544 6.37e-72 239
MS.gene023309.t1 MTR_5g094450 34.230 409 234 12 137 515 129 532 4.61e-68 228
MS.gene023309.t1 MTR_3g056110 36.096 374 211 9 163 515 176 542 1.14e-67 228
MS.gene023309.t1 MTR_1g029420 36.870 377 204 11 163 515 226 592 1.08e-65 223
MS.gene023309.t1 MTR_7g074650 36.800 375 210 11 166 526 442 803 2.30e-65 227
MS.gene023309.t1 MTR_3g089055 35.544 377 209 11 163 515 204 570 3.06e-65 222
MS.gene023309.t1 MTR_3g089055 35.544 377 209 11 163 515 199 565 4.74e-65 221
MS.gene023309.t1 MTR_4g133660 34.409 372 219 9 163 514 176 542 6.16e-65 220
MS.gene023309.t1 MTR_4g104020 32.000 500 279 16 52 515 46 520 3.69e-64 218
MS.gene023309.t1 MTR_4g102790 34.715 386 217 11 155 514 285 661 4.05e-64 221
MS.gene023309.t1 MTR_2g082090 35.638 376 208 12 163 514 213 578 8.34e-62 213
MS.gene023309.t1 MTR_3g022830 29.551 423 218 10 163 516 26 437 4.75e-58 199
MS.gene023309.t1 MTR_2g034250 32.821 390 236 6 151 516 199 586 1.58e-57 201
MS.gene023309.t1 MTR_1g069725 30.377 451 265 14 77 514 48 462 9.27e-56 194
MS.gene023309.t1 MTR_2g026250 29.815 379 241 8 157 515 223 596 4.65e-55 195
MS.gene023309.t1 MTR_2g034260 30.435 391 243 6 151 516 199 585 4.62e-53 189
MS.gene023309.t1 MTR_1g096030 31.750 400 219 16 156 515 74 459 1.96e-52 185
MS.gene023309.t1 MTR_2g034280 30.670 388 244 7 151 516 192 576 1.97e-51 185
MS.gene023309.t1 MTR_2g097410 32.796 372 227 8 163 514 372 740 2.74e-51 187
MS.gene023309.t1 MTR_7g062120 32.706 425 236 13 132 517 329 742 2.74e-51 187
MS.gene023309.t1 MTR_2g097467 32.134 389 237 10 142 514 276 653 1.72e-50 183
MS.gene023309.t1 MTR_2g097310 30.709 381 241 8 152 514 262 637 6.64e-50 181
MS.gene023309.t1 MTR_4g076020 33.880 366 202 12 166 515 88 429 2.37e-49 176
MS.gene023309.t1 MTR_4g064200 31.806 371 228 9 163 514 285 649 3.48e-49 180
MS.gene023309.t1 MTR_2g097350 30.184 381 243 7 152 514 264 639 5.76e-49 179
MS.gene023309.t1 MTR_2g097473 31.467 375 237 9 156 514 283 653 1.47e-48 178
MS.gene023309.t1 MTR_4g064160 31.300 377 236 9 157 514 311 683 2.14e-48 178
MS.gene023309.t1 MTR_2g097390 29.921 381 244 8 152 514 265 640 5.63e-48 176
MS.gene023309.t1 MTR_4g064180 29.224 438 268 11 101 513 204 624 8.15e-48 176
MS.gene023309.t1 MTR_2g097380 29.457 387 239 9 137 514 199 560 1.28e-47 174
MS.gene023309.t1 MTR_4g064120 29.385 439 266 12 101 513 204 624 1.38e-47 175
MS.gene023309.t1 MTR_4g064150 30.405 444 269 13 101 514 299 732 7.08e-47 174
MS.gene023309.t1 MTR_2g097463 30.163 368 237 8 163 514 291 654 1.93e-46 172
MS.gene023309.t1 MTR_4g097080 28.150 373 245 7 163 515 134 503 1.54e-45 167
MS.gene023309.t1 MTR_7g109580 28.125 384 252 6 151 516 178 555 5.14e-43 161
MS.gene023309.t1 MTR_5g058860 32.080 399 226 15 151 514 108 496 5.56e-43 160
MS.gene023309.t1 MTR_5g015490 29.551 379 238 8 163 515 115 490 2.14e-42 158
MS.gene023309.t1 MTR_3g072710 30.732 410 235 15 150 521 108 506 1.72e-41 156
MS.gene023309.t1 MTR_4g077760 28.390 472 288 18 70 515 108 555 2.11e-41 156
MS.gene023309.t1 MTR_8g093070 30.221 407 242 15 131 514 108 495 1.58e-37 145
MS.gene023309.t1 MTR_1g086970 25.191 393 265 7 148 516 92 479 4.09e-37 143
MS.gene023309.t1 MTR_0092s0100 28.649 370 241 8 159 515 372 731 4.60e-36 143
MS.gene023309.t1 MTR_0092s0100 28.649 370 241 8 159 515 369 728 5.23e-36 143
MS.gene023309.t1 MTR_6g047750 29.243 383 229 9 161 515 294 662 2.98e-35 140
MS.gene023309.t1 MTR_2g089100 26.768 396 240 13 163 514 53 442 2.82e-34 135
MS.gene023309.t1 MTR_4g026485 26.243 362 251 6 163 515 270 624 7.81e-34 135
MS.gene023309.t1 MTR_7g057230 29.365 378 229 13 161 514 293 656 5.84e-33 133
MS.gene023309.t1 MTR_5g009080 34.375 224 128 5 150 361 44 260 6.33e-33 131
MS.gene023309.t1 MTR_5g009080 35.526 152 95 2 366 515 328 478 1.28e-28 119
MS.gene023309.t1 MTR_4g076140 33.929 224 129 5 150 361 36 252 1.10e-30 125
MS.gene023309.t1 MTR_4g076140 35.762 151 94 2 367 515 320 469 1.11e-27 116
MS.gene023309.t1 MTR_2g099110 27.273 385 240 10 163 515 75 451 1.84e-30 124
MS.gene023309.t1 MTR_5g015950 24.940 417 241 11 163 514 36 445 2.75e-28 117
MS.gene023309.t1 MTR_3g053270 24.940 417 241 11 163 514 36 445 2.75e-28 117
MS.gene023309.t1 MTR_4g095500 24.879 414 243 12 163 514 60 467 1.92e-24 106
MS.gene023309.t1 MTR_8g020840 25.323 387 247 11 163 516 172 549 3.00e-24 106
MS.gene023309.t1 MTR_8g077940 25.532 376 248 11 159 515 179 541 1.80e-22 101
MS.gene023309.t1 MTR_3g022580 39.130 115 62 3 406 516 74 184 2.06e-18 83.6
MS.gene023309.t1 MTR_3g022005 38.542 96 57 1 421 516 33 126 2.83e-17 78.6
MS.gene023309.t1 MTR_4g122240 46.392 97 46 2 154 250 27 117 1.49e-16 76.3
MS.gene023309.t1 MTR_1g106590 31.452 124 73 2 156 267 124 247 9.34e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023309.t1 AT1G14920 69.407 523 130 9 13 517 21 531 0.0 721
MS.gene023309.t1 AT2G01570 65.179 560 133 10 13 519 35 585 0.0 701
MS.gene023309.t1 AT1G66350 61.100 509 167 9 13 516 25 507 0.0 631
MS.gene023309.t1 AT3G03450 60.039 513 179 6 21 517 45 547 0.0 626
MS.gene023309.t1 AT5G17490 56.335 513 185 9 19 518 33 519 0.0 573
MS.gene023309.t1 AT2G04890 34.694 392 216 13 147 515 39 413 2.85e-70 231
MS.gene023309.t1 AT5G48150 36.533 375 209 11 163 515 123 490 1.16e-69 231
MS.gene023309.t1 AT5G48150 36.533 375 209 11 163 515 123 490 1.16e-69 231
MS.gene023309.t1 AT5G48150 36.533 375 209 11 163 515 123 490 1.16e-69 231
MS.gene023309.t1 AT5G48150 36.533 375 209 11 163 515 123 490 1.16e-69 231
MS.gene023309.t1 AT1G50600 34.946 372 218 9 163 515 160 526 1.94e-66 224
MS.gene023309.t1 AT1G50600 34.759 374 216 8 163 515 231 597 4.95e-66 224
MS.gene023309.t1 AT1G50600 34.759 374 216 8 163 515 235 601 5.88e-66 224
MS.gene023309.t1 AT3G54220 38.095 378 204 12 154 514 285 649 6.42e-66 226
MS.gene023309.t1 AT5G41920 34.217 415 228 15 128 515 4 400 1.48e-60 205
MS.gene023309.t1 AT4G17230 34.293 382 219 9 157 515 153 525 1.93e-57 200
MS.gene023309.t1 AT4G17230 34.293 382 219 9 157 515 153 525 1.93e-57 200
MS.gene023309.t1 AT1G63100 33.418 395 219 13 154 514 244 628 7.14e-57 201
MS.gene023309.t1 AT1G63100 33.418 395 219 13 154 514 269 653 1.61e-56 200
MS.gene023309.t1 AT1G21450 33.687 377 217 11 163 515 226 593 2.74e-56 198
MS.gene023309.t1 AT5G59450 30.971 381 229 11 163 517 228 600 5.73e-52 187
MS.gene023309.t1 AT1G07530 33.244 373 223 11 163 514 397 764 2.33e-50 184
MS.gene023309.t1 AT3G46600 32.361 377 225 10 163 517 5 373 3.10e-50 177
MS.gene023309.t1 AT1G55580 37.333 300 169 8 230 515 151 445 9.90e-50 177
MS.gene023309.t1 AT2G29060 32.275 378 222 11 163 514 285 654 5.44e-49 179
MS.gene023309.t1 AT3G46600 32.361 377 225 10 163 517 181 549 6.67e-49 177
MS.gene023309.t1 AT2G29060 32.275 378 222 11 163 514 322 691 7.40e-49 179
MS.gene023309.t1 AT3G46600 32.361 377 225 10 163 517 213 581 7.65e-49 178
MS.gene023309.t1 AT5G66770 33.957 374 224 14 159 515 217 584 4.03e-45 167
MS.gene023309.t1 AT4G08250 29.949 394 241 13 153 520 98 482 1.66e-42 158
MS.gene023309.t1 AT2G37650 30.504 377 227 10 163 515 336 701 2.58e-42 161
MS.gene023309.t1 AT2G37650 30.504 377 227 10 163 515 348 713 3.14e-42 161
MS.gene023309.t1 AT4G37650 28.312 385 242 7 163 515 147 529 7.64e-41 154
MS.gene023309.t1 AT2G29065 28.021 389 229 11 163 514 253 627 3.86e-40 154
MS.gene023309.t1 AT3G50650 33.043 345 210 11 189 515 201 542 7.97e-40 152
MS.gene023309.t1 AT1G07520 27.851 377 244 8 163 514 155 528 1.47e-39 151
MS.gene023309.t1 AT1G07520 27.851 377 244 8 163 514 155 528 1.47e-39 151
MS.gene023309.t1 AT1G07520 27.851 377 244 8 163 514 320 693 3.59e-39 152
MS.gene023309.t1 AT1G07520 27.851 377 244 8 163 514 319 692 3.74e-39 152
MS.gene023309.t1 AT5G52510 28.645 391 226 13 161 515 267 640 1.09e-36 144
MS.gene023309.t1 AT3G49950 27.013 385 240 11 163 514 31 407 7.96e-34 132
MS.gene023309.t1 AT1G50420 31.679 262 145 6 139 374 23 276 1.46e-31 127
MS.gene023309.t1 AT1G50420 39.610 154 90 3 364 515 327 479 1.73e-29 121
MS.gene023309.t1 AT2G45160 29.683 347 206 11 191 515 309 639 1.94e-31 129
MS.gene023309.t1 AT4G00150 27.224 371 239 7 157 515 203 554 7.85e-30 123
MS.gene023309.t1 AT3G60630 31.544 298 186 8 228 515 333 622 2.49e-29 122
MS.gene023309.t1 AT4G36710 26.579 380 248 13 156 516 119 486 3.36e-25 109
MS.gene023309.t1 AT3G13840 24.548 387 233 13 163 515 149 510 4.16e-17 84.7

Find 135 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGCAACAGATTCCGTTTC+CGG 0.220956 4.2:-15742617 None:intergenic
CGGATTCTCTCCGGTTCATT+TGG 0.233363 4.2:+15742976 MS.gene023309:CDS
ATTAGGTTGAGGGTCAAAAT+TGG 0.282718 4.2:-15741850 None:intergenic
TAGGACAAGTTTCATAGAAA+TGG 0.309171 4.2:-15742268 None:intergenic
CGGAGTGTTGACGGCGTAGA+TGG 0.332290 4.2:-15741925 None:intergenic
GTCGTTATCGTCGGAGTTAA+CGG 0.337265 4.2:-15741880 None:intergenic
TGTGTCTCCACCACCATTAC+AGG 0.339800 4.2:-15742026 None:intergenic
AACGACCTTAACGCCATTCC+TGG 0.342480 4.2:+15741897 MS.gene023309:CDS
CGCCGGTGGGGATGGTTACA+AGG 0.354252 4.2:+15743042 MS.gene023309:CDS
GAGTTAACGGAAGGGATATT+AGG 0.357506 4.2:-15741867 None:intergenic
TCGTCTTGTTCATAGTTTGA+TGG 0.362969 4.2:+15742061 MS.gene023309:CDS
GTGATAAATCAGGTGTTTGT+TGG 0.365737 4.2:+15741604 MS.gene023309:CDS
GGATTCTCTCCGGTTCATTT+GGG 0.381628 4.2:+15742977 MS.gene023309:CDS
GCTATGTCGGAGGTTTATCT+AGG 0.387843 4.2:+15742860 MS.gene023309:CDS
TCTAACGGTTGCCGAGGCTT+TGG 0.390263 4.2:+15742112 MS.gene023309:CDS
GCAGTGGCCGGCGCTTATGC+AGG 0.393651 4.2:+15742391 MS.gene023309:CDS
TAGAAGATTCTCTTCCTTGT+CGG 0.417772 4.2:+15741949 MS.gene023309:CDS
CACTGAACCAGTGGAGGAAC+CGG 0.419497 4.2:+15742942 MS.gene023309:CDS
CAAATGAACCGGAGAGAATC+CGG 0.428302 4.2:-15742975 None:intergenic
CAGGCGCTTGCTTTACGTCC+TGG 0.433094 4.2:+15742410 MS.gene023309:CDS
CGACGCAGGAGGTCCGATTC+CGG 0.435814 4.2:-15742456 None:intergenic
TGATAAATCAGGTGTTTGTT+GGG 0.437638 4.2:+15741605 MS.gene023309:CDS
GATGAACTCTTAGCTGTAGT+TGG 0.440876 4.2:+15741648 MS.gene023309:CDS
ATCCCGCTGATATCTCTAAC+TGG 0.443018 4.2:+15741811 MS.gene023309:CDS
ATCTTCTAACGGAGTGTTGA+CGG 0.444247 4.2:-15741934 None:intergenic
TGAACTCCGGTCACCGGAAA+CGG 0.454553 4.2:+15742604 MS.gene023309:CDS
CTTTGATTGCAACTTCAGCT+TGG 0.454865 4.2:+15743110 MS.gene023309:CDS
GGAAGACGACGGTGGTGGTA+TGG 0.462243 4.2:+15741626 MS.gene023309:CDS
AATTAAACCGGTTCCTCCAC+TGG 0.463276 4.2:-15742949 None:intergenic
CTTTACCAGGAATGGCGTTA+AGG 0.464496 4.2:-15741902 None:intergenic
CAGATTCCGTTTCCGGTGAC+CGG 0.464768 4.2:-15742610 None:intergenic
ATCTTCAACAAGTGGGTTGG+AGG 0.466369 4.2:+15742492 MS.gene023309:CDS
TTTCTGTCATTCGTCAGATT+CGG 0.467127 4.2:+15742699 MS.gene023309:CDS
ACAACTTGAACAAGCTATGA+TGG 0.469434 4.2:+15741719 MS.gene023309:CDS
CGGAAGGGATATTAGGTTGA+GGG 0.478041 4.2:-15741860 None:intergenic
GATCTGAAGCGAATCGGAAA+CGG 0.478124 4.2:-15742246 None:intergenic
AGTGAAGTGACTCGGTGAAC+CGG 0.480174 4.2:-15742781 None:intergenic
TCAACAACGGTGACAATCTC+CGG 0.482534 4.2:-15742722 None:intergenic
CGAAAAGCAGGAGGACCACC+AGG 0.492013 4.2:-15742428 None:intergenic
AAACTCGCCGCTGCTAACTC+AGG 0.493547 4.2:+15743133 MS.gene023309:CDS
AGTAGTGGAAGTGATAAATC+AGG 0.494290 4.2:+15741594 MS.gene023309:CDS
CATAAACTTAACGCGCGTCC+CGG 0.499031 4.2:+15742659 MS.gene023309:CDS
ATGCTTCGATGCTTGAACTC+CGG 0.504484 4.2:+15742591 MS.gene023309:CDS
ATGGGTGTCTTATGCTTGGT+TGG 0.506719 4.2:+15743077 MS.gene023309:CDS
AGAATCCGGCTGAATTAAAC+CGG 0.508366 4.2:-15742961 None:intergenic
TAATTCAGCCGGATTCTCTC+CGG 0.509443 4.2:+15742967 MS.gene023309:CDS
ACGGAAGGGATATTAGGTTG+AGG 0.513663 4.2:-15741861 None:intergenic
GATGCTTGAACTCCGGTCAC+CGG 0.513844 4.2:+15742598 MS.gene023309:CDS
AACTCGCCGCTGCTAACTCA+GGG 0.514322 4.2:+15743134 MS.gene023309:CDS
AACACTTTCTCCAACGCGCC+GGG 0.514572 4.2:-15742677 None:intergenic
ATTCCGGTCAACCGAAAAGC+AGG 0.515015 4.2:-15742440 None:intergenic
CAAGCTGAAGTTGCAATCAA+AGG 0.518495 4.2:-15743109 None:intergenic
GCGTTAGATCCCAAATGAAC+CGG 0.519078 4.2:-15742986 None:intergenic
CTCAACTCTATTTGACTCGC+TGG 0.522599 4.2:+15742808 MS.gene023309:CDS
GTGGTGGAGACACAAGAGAA+AGG 0.525063 4.2:+15742035 MS.gene023309:CDS
GAAATGGATCTGAAGCGAAT+CGG 0.531356 4.2:-15742252 None:intergenic
ATCATCTTCAACAAGTGGGT+TGG 0.533125 4.2:+15742489 MS.gene023309:CDS
ACGGCGTAGATGGCTTTACC+AGG 0.536683 4.2:-15741915 None:intergenic
CATGTTCAGTTTGAGTATCG+TGG 0.537387 4.2:+15742539 MS.gene023309:CDS
GTCAACAACTCGTCCTGTAA+TGG 0.538427 4.2:+15742013 MS.gene023309:CDS
AACTCGTCCTGTAATGGTGG+TGG 0.540144 4.2:+15742019 MS.gene023309:CDS
AACTCTATTTGACTCGCTGG+AGG 0.540788 4.2:+15742811 MS.gene023309:CDS
TAATCAAGGGATGCAGTGGC+CGG 0.544704 4.2:+15742379 MS.gene023309:CDS
TGGTGGATTCATCATGGTCA+TGG 0.546680 4.2:-15741752 None:intergenic
AGGAACCGGTTTAATTCAGC+CGG 0.550915 4.2:+15742956 MS.gene023309:CDS
CGTGGTGGCGTGTGAAGGAA+CGG 0.554031 4.2:+15742898 MS.gene023309:CDS
ACCCACTTGTTGAAGATGAT+CGG 0.554084 4.2:-15742486 None:intergenic
GGCGTGTGAAGGAACGGACC+GGG 0.557867 4.2:+15742904 MS.gene023309:CDS
CGATTAATCAAGGGATGCAG+TGG 0.559478 4.2:+15742375 MS.gene023309:CDS
TCCGACATAGCTTTATCTTG+CGG 0.561722 4.2:-15742848 None:intergenic
TGGCGTGTGAAGGAACGGAC+CGG 0.563132 4.2:+15742903 MS.gene023309:CDS
TAACGCGCGTCCCGGCGCGT+TGG 0.564884 4.2:+15742667 MS.gene023309:CDS
CTCCGATCATCTTCAACAAG+TGG 0.566538 4.2:+15742484 MS.gene023309:CDS
GCAAGCGCCTGCATAAGCGC+CGG 0.567370 4.2:-15742398 None:intergenic
GTGTCTCATGACGTTCAACC+CGG 0.568132 4.2:-15742922 None:intergenic
TGGTGGAGACACAAGAGAAA+GGG 0.575014 4.2:+15742036 MS.gene023309:CDS
GTGAACCGGTCTAGAAAAGC+CGG 0.575536 4.2:-15742767 None:intergenic
GTAGATGGCTTTACCAGGAA+TGG 0.576204 4.2:-15741910 None:intergenic
CTATGTCGGAGGTTTATCTA+GGG 0.578182 4.2:+15742861 MS.gene023309:CDS
GAGGCTTTGGTGAAACAGAT+CGG 0.578980 4.2:+15742125 MS.gene023309:CDS
AGCCAGTTAGAGATATCAGC+GGG 0.579302 4.2:-15741813 None:intergenic
AGAAGCAAATCACAATGGAC+CGG 0.580795 4.2:+15742748 MS.gene023309:CDS
AATGATGGGTGTCTTATGCT+TGG 0.582762 4.2:+15743073 MS.gene023309:CDS
CGCCGCTGCTAACTCAGGGG+TGG 0.584518 4.2:+15743138 MS.gene023309:CDS
GGCGTTAAGGTCGTTATCGT+CGG 0.584752 4.2:-15741889 None:intergenic
TACAAGGTGGAAGAAAATGA+TGG 0.584899 4.2:+15743058 MS.gene023309:CDS
CAACTTGAACAAGCTATGAT+GGG 0.585116 4.2:+15741720 MS.gene023309:CDS
GGTTTAGCAAGAAGAATCTA+CGG 0.585286 4.2:+15742206 MS.gene023309:CDS
ACTCTATTTGACTCGCTGGA+GGG 0.586867 4.2:+15742812 MS.gene023309:CDS
GAACATGAAGATATGAGTAG+TGG 0.588847 4.2:+15741579 None:intergenic
TGTTGGGAAGACGACGGTGG+TGG 0.595930 4.2:+15741621 MS.gene023309:CDS
TGAAAGATGTTGAGCGATGG+TGG 0.597047 4.2:-15741769 None:intergenic
TGTCATTCGTCAGATTCGGC+CGG 0.598760 4.2:+15742703 MS.gene023309:CDS
CAAAGTGAAATCTTCAGACA+TGG 0.601830 4.2:+15741674 MS.gene023309:CDS
CGGAATCGGACCTCCTGCGT+CGG 0.608126 4.2:+15742457 MS.gene023309:CDS
TACTTAGCGGTATCACAAGA+AGG 0.608334 4.2:+15742149 MS.gene023309:CDS
AAACACTTTCTCCAACGCGC+CGG 0.613410 4.2:-15742678 None:intergenic
GAAACAGATCGGATACTTAG+CGG 0.614040 4.2:+15742136 MS.gene023309:CDS
TTATCGTCGGAGTTAACGGA+AGG 0.614612 4.2:-15741876 None:intergenic
ATTTGCTTCTTGCTCAACAA+CGG 0.614962 4.2:-15742735 None:intergenic
ACCGCAAGATAAAGCTATGT+CGG 0.617535 4.2:+15742847 MS.gene023309:CDS
CACCTTGTAACCATCCCCAC+CGG 0.617871 4.2:-15743044 None:intergenic
TCCGATCATCTTCAACAAGT+GGG 0.621530 4.2:+15742485 MS.gene023309:CDS
ACAAGGTGGAAGAAAATGAT+GGG 0.622695 4.2:+15743059 MS.gene023309:CDS
TGCAACGTGGTGGCGTGTGA+AGG 0.623126 4.2:+15742893 MS.gene023309:CDS
GATCTGTTTCACCAAAGCCT+CGG 0.623631 4.2:-15742123 None:intergenic
ACCGGAAACGGAATCTGTTG+CGG 0.625355 4.2:+15742616 MS.gene023309:CDS
AGTTGTTGACTCAGTAGTAA+CGG 0.629850 4.2:-15742000 None:intergenic
ATGAGACACTGAACCAGTGG+AGG 0.630427 4.2:+15742936 MS.gene023309:CDS
TGATCGGAGTTATCCGACGC+AGG 0.637109 4.2:-15742470 None:intergenic
AACAACTCGTCCTGTAATGG+TGG 0.638478 4.2:+15742016 MS.gene023309:CDS
CAAGGAAGAGAATCTTCTAA+CGG 0.641855 4.2:-15741945 None:intergenic
TGACTCAGTAGTAACGGTAA+CGG 0.644967 4.2:-15741994 None:intergenic
GAATAATCTAACGGTTGCCG+AGG 0.647157 4.2:+15742106 MS.gene023309:CDS
TGTTGAACAGAATAATCTAA+CGG 0.647816 4.2:+15742097 MS.gene023309:CDS
GTCATGAGACACTGAACCAG+TGG 0.649901 4.2:+15742933 MS.gene023309:CDS
ATCAGCGGGATTGTAATGAA+CGG 0.651269 4.2:-15741799 None:intergenic
GCGCTTGCTTTACGTCCTGG+TGG 0.654464 4.2:+15742413 MS.gene023309:CDS
GAGCGATGGTGGATTCATCA+TGG 0.655401 4.2:-15741758 None:intergenic
AACCACCCCTGAGTTAGCAG+CGG 0.663533 4.2:-15743140 None:intergenic
CCGGTCAACCGAAAAGCAGG+AGG 0.668991 4.2:-15742437 None:intergenic
GTTTGAAAGATGTTGAGCGA+TGG 0.672287 4.2:-15741772 None:intergenic
GAATCGGAAACGGAATGCTG+TGG 0.677665 4.2:-15742236 None:intergenic
GAGCAAGAAGCAAATCACAA+TGG 0.679555 4.2:+15742743 MS.gene023309:CDS
GAAGCAAATCTGCAACGTGG+TGG 0.682123 4.2:+15742883 MS.gene023309:CDS
TGAGTAATAGTGAAGTGACT+CGG 0.685386 4.2:-15742789 None:intergenic
GTTTGTTGGGAAGACGACGG+TGG 0.693905 4.2:+15741618 MS.gene023309:CDS
AAGCCAGTTAGAGATATCAG+CGG 0.702780 4.2:-15741814 None:intergenic
TCGGAGTTATCCGACGCAGG+AGG 0.703403 4.2:-15742467 None:intergenic
TATCGTCGGAGTTAACGGAA+GGG 0.705280 4.2:-15741875 None:intergenic
GCAAGATAAAGCTATGTCGG+AGG 0.706977 4.2:+15742850 MS.gene023309:CDS
ACTCGCCGCTGCTAACTCAG+GGG 0.713660 4.2:+15743135 MS.gene023309:CDS
GGTGTTTGTTGGGAAGACGA+CGG 0.714285 4.2:+15741615 MS.gene023309:CDS
CGGTGGGGATGGTTACAAGG+TGG 0.744680 4.2:+15743045 MS.gene023309:CDS
AGGGAAGCAAATCTGCAACG+TGG 0.782429 4.2:+15742880 MS.gene023309:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATTTTTCGATTAATCA+AGG + chr4.2:15742365-15742384 MS.gene023309:CDS 20.0%
!!! TTGATTTTTCGATTAATCAA+GGG + chr4.2:15742366-15742385 MS.gene023309:CDS 20.0%
! TGTTGAACAGAATAATCTAA+CGG + chr4.2:15742097-15742116 MS.gene023309:CDS 25.0%
!! AAGTGAGCGAATTTTAAATT+AGG - chr4.2:15742290-15742309 None:intergenic 25.0%
TAGGACAAGTTTCATAGAAA+TGG - chr4.2:15742271-15742290 None:intergenic 30.0%
TGATAAATCAGGTGTTTGTT+GGG + chr4.2:15741605-15741624 MS.gene023309:CDS 30.0%
ACAACTTGAACAAGCTATGA+TGG + chr4.2:15741719-15741738 MS.gene023309:CDS 35.0%
ACAAGGTGGAAGAAAATGAT+GGG + chr4.2:15743059-15743078 MS.gene023309:CDS 35.0%
ATTTGCTTCTTGCTCAACAA+CGG - chr4.2:15742738-15742757 None:intergenic 35.0%
CAAAGTGAAATCTTCAGACA+TGG + chr4.2:15741674-15741693 MS.gene023309:CDS 35.0%
CAACTTGAACAAGCTATGAT+GGG + chr4.2:15741720-15741739 MS.gene023309:CDS 35.0%
CAAGGAAGAGAATCTTCTAA+CGG - chr4.2:15741948-15741967 None:intergenic 35.0%
GGTTTAGCAAGAAGAATCTA+CGG + chr4.2:15742206-15742225 MS.gene023309:CDS 35.0%
GTGATAAATCAGGTGTTTGT+TGG + chr4.2:15741604-15741623 MS.gene023309:CDS 35.0%
TACAAGGTGGAAGAAAATGA+TGG + chr4.2:15743058-15743077 MS.gene023309:CDS 35.0%
TAGAAGATTCTCTTCCTTGT+CGG + chr4.2:15741949-15741968 MS.gene023309:CDS 35.0%
TGAGTAATAGTGAAGTGACT+CGG - chr4.2:15742792-15742811 None:intergenic 35.0%
TTTCTGTCATTCGTCAGATT+CGG + chr4.2:15742699-15742718 MS.gene023309:CDS 35.0%
! AGTAGTGGAAGTGATAAATC+AGG + chr4.2:15741594-15741613 MS.gene023309:CDS 35.0%
! TCGTCTTGTTCATAGTTTGA+TGG + chr4.2:15742061-15742080 MS.gene023309:CDS 35.0%
!! AGTTGTTGACTCAGTAGTAA+CGG - chr4.2:15742003-15742022 None:intergenic 35.0%
!! ATTAGGTTGAGGGTCAAAAT+TGG - chr4.2:15741853-15741872 None:intergenic 35.0%
!! CTTTTGATTCTTTTCCGACA+AGG - chr4.2:15741966-15741985 None:intergenic 35.0%
!! GCTATTCTAGAAGCTTTTCA+AGG + chr4.2:15742323-15742342 MS.gene023309:CDS 35.0%
!!! GTTGCTACTTATTTTGCTGA+AGG + chr4.2:15742185-15742204 MS.gene023309:CDS 35.0%
AAGCCAGTTAGAGATATCAG+CGG - chr4.2:15741817-15741836 None:intergenic 40.0%
ACCGCAAGATAAAGCTATGT+CGG + chr4.2:15742847-15742866 MS.gene023309:CDS 40.0%
AGAAGCAAATCACAATGGAC+CGG + chr4.2:15742748-15742767 MS.gene023309:CDS 40.0%
AGAATCCGGCTGAATTAAAC+CGG - chr4.2:15742964-15742983 None:intergenic 40.0%
ATCAGCGGGATTGTAATGAA+CGG - chr4.2:15741802-15741821 None:intergenic 40.0%
ATCATCTTCAACAAGTGGGT+TGG + chr4.2:15742489-15742508 MS.gene023309:CDS 40.0%
CAAGCTGAAGTTGCAATCAA+AGG - chr4.2:15743112-15743131 None:intergenic 40.0%
CTATGTCGGAGGTTTATCTA+GGG + chr4.2:15742861-15742880 MS.gene023309:CDS 40.0%
CTTTGATTGCAACTTCAGCT+TGG + chr4.2:15743110-15743129 MS.gene023309:CDS 40.0%
GAAACAGATCGGATACTTAG+CGG + chr4.2:15742136-15742155 MS.gene023309:CDS 40.0%
GAGCAAGAAGCAAATCACAA+TGG + chr4.2:15742743-15742762 MS.gene023309:CDS 40.0%
GAGTTAACGGAAGGGATATT+AGG - chr4.2:15741870-15741889 None:intergenic 40.0%
GATGAACTCTTAGCTGTAGT+TGG + chr4.2:15741648-15741667 MS.gene023309:CDS 40.0%
GTTTGAAAGATGTTGAGCGA+TGG - chr4.2:15741775-15741794 None:intergenic 40.0%
TACTTAGCGGTATCACAAGA+AGG + chr4.2:15742149-15742168 MS.gene023309:CDS 40.0%
TCCGATCATCTTCAACAAGT+GGG + chr4.2:15742485-15742504 MS.gene023309:CDS 40.0%
TGACTCAGTAGTAACGGTAA+CGG - chr4.2:15741997-15742016 None:intergenic 40.0%
! ACCCACTTGTTGAAGATGAT+CGG - chr4.2:15742489-15742508 None:intergenic 40.0%
! ATGTTGTTAGCACTTTTCGC+CGG + chr4.2:15743025-15743044 MS.gene023309:CDS 40.0%
! CATGTTCAGTTTGAGTATCG+TGG + chr4.2:15742539-15742558 MS.gene023309:CDS 40.0%
! GAAATGGATCTGAAGCGAAT+CGG - chr4.2:15742255-15742274 None:intergenic 40.0%
!! AATGATGGGTGTCTTATGCT+TGG + chr4.2:15743073-15743092 MS.gene023309:CDS 40.0%
!! ATCTTCTAACGGAGTGTTGA+CGG - chr4.2:15741937-15741956 None:intergenic 40.0%
!! GTTTGAGTATCGTGGTTTTG+TGG + chr4.2:15742547-15742566 MS.gene023309:CDS 40.0%
!! TCCGACATAGCTTTATCTTG+CGG - chr4.2:15742851-15742870 None:intergenic 40.0%
AACAACTCGTCCTGTAATGG+TGG + chr4.2:15742016-15742035 MS.gene023309:CDS 45.0%
AGCCAGTTAGAGATATCAGC+GGG - chr4.2:15741816-15741835 None:intergenic 45.0%
ATCCCGCTGATATCTCTAAC+TGG + chr4.2:15741811-15741830 MS.gene023309:CDS 45.0%
ATGCTTCGATGCTTGAACTC+CGG + chr4.2:15742591-15742610 MS.gene023309:CDS 45.0%
ATGGGTGTCTTATGCTTGGT+TGG + chr4.2:15743077-15743096 MS.gene023309:CDS 45.0%
CAAATGAACCGGAGAGAATC+CGG - chr4.2:15742978-15742997 None:intergenic 45.0%
CGATTAATCAAGGGATGCAG+TGG + chr4.2:15742375-15742394 MS.gene023309:CDS 45.0%
CTCCGATCATCTTCAACAAG+TGG + chr4.2:15742484-15742503 MS.gene023309:CDS 45.0%
CTTTACCAGGAATGGCGTTA+AGG - chr4.2:15741905-15741924 None:intergenic 45.0%
GAATAATCTAACGGTTGCCG+AGG + chr4.2:15742106-15742125 MS.gene023309:CDS 45.0%
GATCTGTTTCACCAAAGCCT+CGG - chr4.2:15742126-15742145 None:intergenic 45.0%
GCAAGATAAAGCTATGTCGG+AGG + chr4.2:15742850-15742869 MS.gene023309:CDS 45.0%
GCGTTAGATCCCAAATGAAC+CGG - chr4.2:15742989-15743008 None:intergenic 45.0%
GCTATGTCGGAGGTTTATCT+AGG + chr4.2:15742860-15742879 MS.gene023309:CDS 45.0%
GGATTCTCTCCGGTTCATTT+GGG + chr4.2:15742977-15742996 MS.gene023309:CDS 45.0%
GTCAACAACTCGTCCTGTAA+TGG + chr4.2:15742013-15742032 MS.gene023309:CDS 45.0%
GTCGTTATCGTCGGAGTTAA+CGG - chr4.2:15741883-15741902 None:intergenic 45.0%
TAATTCAGCCGGATTCTCTC+CGG + chr4.2:15742967-15742986 MS.gene023309:CDS 45.0%
TATCGTCGGAGTTAACGGAA+GGG - chr4.2:15741878-15741897 None:intergenic 45.0%
TCAACAACGGTGACAATCTC+CGG - chr4.2:15742725-15742744 None:intergenic 45.0%
TGAAAGATGTTGAGCGATGG+TGG - chr4.2:15741772-15741791 None:intergenic 45.0%
TGGTGGAGACACAAGAGAAA+GGG + chr4.2:15742036-15742055 MS.gene023309:CDS 45.0%
TTATCGTCGGAGTTAACGGA+AGG - chr4.2:15741879-15741898 None:intergenic 45.0%
! AACTCTATTTGACTCGCTGG+AGG + chr4.2:15742811-15742830 MS.gene023309:CDS 45.0%
! AATTAAACCGGTTCCTCCAC+TGG - chr4.2:15742952-15742971 None:intergenic 45.0%
! ACGGAAGGGATATTAGGTTG+AGG - chr4.2:15741864-15741883 None:intergenic 45.0%
! ACTCTATTTGACTCGCTGGA+GGG + chr4.2:15742812-15742831 MS.gene023309:CDS 45.0%
! AGGAACCGGTTTAATTCAGC+CGG + chr4.2:15742956-15742975 MS.gene023309:CDS 45.0%
! CTCAACTCTATTTGACTCGC+TGG + chr4.2:15742808-15742827 MS.gene023309:CDS 45.0%
! GAGGCTTTGGTGAAACAGAT+CGG + chr4.2:15742125-15742144 MS.gene023309:CDS 45.0%
! GATCTGAAGCGAATCGGAAA+CGG - chr4.2:15742249-15742268 None:intergenic 45.0%
! GTAGATGGCTTTACCAGGAA+TGG - chr4.2:15741913-15741932 None:intergenic 45.0%
!! ATCTTCAACAAGTGGGTTGG+AGG + chr4.2:15742492-15742511 MS.gene023309:CDS 45.0%
!! CGGAAGGGATATTAGGTTGA+GGG - chr4.2:15741863-15741882 None:intergenic 45.0%
!! TGGTGGATTCATCATGGTCA+TGG - chr4.2:15741755-15741774 None:intergenic 45.0%
AAACACTTTCTCCAACGCGC+CGG - chr4.2:15742681-15742700 None:intergenic 50.0%
AACGACCTTAACGCCATTCC+TGG + chr4.2:15741897-15741916 MS.gene023309:CDS 50.0%
AACTCGTCCTGTAATGGTGG+TGG + chr4.2:15742019-15742038 MS.gene023309:CDS 50.0%
ACCGCAACAGATTCCGTTTC+CGG - chr4.2:15742620-15742639 None:intergenic 50.0%
ACCGGAAACGGAATCTGTTG+CGG + chr4.2:15742616-15742635 MS.gene023309:CDS 50.0%
AGGGAAGCAAATCTGCAACG+TGG + chr4.2:15742880-15742899 MS.gene023309:CDS 50.0%
ATGAGACACTGAACCAGTGG+AGG + chr4.2:15742936-15742955 MS.gene023309:CDS 50.0%
ATTCCGGTCAACCGAAAAGC+AGG - chr4.2:15742443-15742462 None:intergenic 50.0%
CATAAACTTAACGCGCGTCC+CGG + chr4.2:15742659-15742678 MS.gene023309:CDS 50.0%
CGGATTCTCTCCGGTTCATT+TGG + chr4.2:15742976-15742995 MS.gene023309:CDS 50.0%
GAAGCAAATCTGCAACGTGG+TGG + chr4.2:15742883-15742902 MS.gene023309:CDS 50.0%
GAATCGGAAACGGAATGCTG+TGG - chr4.2:15742239-15742258 None:intergenic 50.0%
GGCGTTAAGGTCGTTATCGT+CGG - chr4.2:15741892-15741911 None:intergenic 50.0%
GTCATGAGACACTGAACCAG+TGG + chr4.2:15742933-15742952 MS.gene023309:CDS 50.0%
GTGAACCGGTCTAGAAAAGC+CGG - chr4.2:15742770-15742789 None:intergenic 50.0%
GTGTCTCATGACGTTCAACC+CGG - chr4.2:15742925-15742944 None:intergenic 50.0%
TAATCAAGGGATGCAGTGGC+CGG + chr4.2:15742379-15742398 MS.gene023309:CDS 50.0%
TGTCATTCGTCAGATTCGGC+CGG + chr4.2:15742703-15742722 MS.gene023309:CDS 50.0%
TGTGTCTCCACCACCATTAC+AGG - chr4.2:15742029-15742048 None:intergenic 50.0%
! GCTTTTCGGTTGACCGGAAT+CGG + chr4.2:15742443-15742462 MS.gene023309:CDS 50.0%
! GGTGTTTGTTGGGAAGACGA+CGG + chr4.2:15741615-15741634 MS.gene023309:CDS 50.0%
! GTGGTGGAGACACAAGAGAA+AGG + chr4.2:15742035-15742054 MS.gene023309:CDS 50.0%
! TGTTAGCACTTTTCGCCGGT+GGG + chr4.2:15743029-15743048 MS.gene023309:CDS 50.0%
! TTGTTAGCACTTTTCGCCGG+TGG + chr4.2:15743028-15743047 MS.gene023309:CDS 50.0%
!! AGTGAAGTGACTCGGTGAAC+CGG - chr4.2:15742784-15742803 None:intergenic 50.0%
!! ATGGACCGGCTTTTCTAGAC+CGG + chr4.2:15742762-15742781 MS.gene023309:CDS 50.0%
!! GAGCGATGGTGGATTCATCA+TGG - chr4.2:15741761-15741780 None:intergenic 50.0%
AAACTCGCCGCTGCTAACTC+AGG + chr4.2:15743133-15743152 MS.gene023309:CDS 55.0%
AACACTTTCTCCAACGCGCC+GGG - chr4.2:15742680-15742699 None:intergenic 55.0%
AACCACCCCTGAGTTAGCAG+CGG - chr4.2:15743143-15743162 None:intergenic 55.0%
AACTCGCCGCTGCTAACTCA+GGG + chr4.2:15743134-15743153 MS.gene023309:CDS 55.0%
CACCTTGTAACCATCCCCAC+CGG - chr4.2:15743047-15743066 None:intergenic 55.0%
CACTGAACCAGTGGAGGAAC+CGG + chr4.2:15742942-15742961 MS.gene023309:CDS 55.0%
CAGATTCCGTTTCCGGTGAC+CGG - chr4.2:15742613-15742632 None:intergenic 55.0%
GATGCTTGAACTCCGGTCAC+CGG + chr4.2:15742598-15742617 MS.gene023309:CDS 55.0%
TCTAACGGTTGCCGAGGCTT+TGG + chr4.2:15742112-15742131 MS.gene023309:CDS 55.0%
TGAACTCCGGTCACCGGAAA+CGG + chr4.2:15742604-15742623 MS.gene023309:CDS 55.0%
TGATCGGAGTTATCCGACGC+AGG - chr4.2:15742473-15742492 None:intergenic 55.0%
! ACGGCGTAGATGGCTTTACC+AGG - chr4.2:15741918-15741937 None:intergenic 55.0%
! GTTAGCACTTTTCGCCGGTG+GGG + chr4.2:15743030-15743049 MS.gene023309:CDS 55.0%
! GTTTGTTGGGAAGACGACGG+TGG + chr4.2:15741618-15741637 MS.gene023309:CDS 55.0%
!! CCTCCTGCTTTTCGGTTGAC+CGG + chr4.2:15742437-15742456 MS.gene023309:CDS 55.0%
!! CTGGTGGTCCTCCTGCTTTT+CGG + chr4.2:15742429-15742448 MS.gene023309:CDS 55.0%
ACTCGCCGCTGCTAACTCAG+GGG + chr4.2:15743135-15743154 MS.gene023309:CDS 60.0%
CAGGCGCTTGCTTTACGTCC+TGG + chr4.2:15742410-15742429 MS.gene023309:CDS 60.0%
CCGGTCAACCGAAAAGCAGG+AGG - chr4.2:15742440-15742459 None:intergenic 60.0%
CGAAAAGCAGGAGGACCACC+AGG - chr4.2:15742431-15742450 None:intergenic 60.0%
CGGTGGGGATGGTTACAAGG+TGG + chr4.2:15743045-15743064 MS.gene023309:CDS 60.0%
GCGCTTGCTTTACGTCCTGG+TGG + chr4.2:15742413-15742432 MS.gene023309:CDS 60.0%
TCGGAGTTATCCGACGCAGG+AGG - chr4.2:15742470-15742489 None:intergenic 60.0%
TGGCGTGTGAAGGAACGGAC+CGG + chr4.2:15742903-15742922 MS.gene023309:CDS 60.0%
TGTTGGGAAGACGACGGTGG+TGG + chr4.2:15741621-15741640 MS.gene023309:CDS 60.0%
! CGTGGTGGCGTGTGAAGGAA+CGG + chr4.2:15742898-15742917 MS.gene023309:CDS 60.0%
! GGAAGACGACGGTGGTGGTA+TGG + chr4.2:15741626-15741645 MS.gene023309:CDS 60.0%
! TGCAACGTGGTGGCGTGTGA+AGG + chr4.2:15742893-15742912 MS.gene023309:CDS 60.0%
!! CGGAGTGTTGACGGCGTAGA+TGG - chr4.2:15741928-15741947 None:intergenic 60.0%
CGACGCAGGAGGTCCGATTC+CGG - chr4.2:15742459-15742478 None:intergenic 65.0%
CGCCGGTGGGGATGGTTACA+AGG + chr4.2:15743042-15743061 MS.gene023309:CDS 65.0%
CGGAATCGGACCTCCTGCGT+CGG + chr4.2:15742457-15742476 MS.gene023309:CDS 65.0%
GCAAGCGCCTGCATAAGCGC+CGG - chr4.2:15742401-15742420 None:intergenic 65.0%
GGCGTGTGAAGGAACGGACC+GGG + chr4.2:15742904-15742923 MS.gene023309:CDS 65.0%
! GCACTTTTCGCCGGTGGGGA+TGG + chr4.2:15743034-15743053 MS.gene023309:CDS 65.0%
CGCCGCTGCTAACTCAGGGG+TGG + chr4.2:15743138-15743157 MS.gene023309:CDS 70.0%
GCAGTGGCCGGCGCTTATGC+AGG + chr4.2:15742391-15742410 MS.gene023309:CDS 70.0%
TAACGCGCGTCCCGGCGCGT+TGG + chr4.2:15742667-15742686 MS.gene023309:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 15741591 15743171 15741591 ID=MS.gene023309
chr4.2 mRNA 15741591 15743171 15741591 ID=MS.gene023309.t1;Parent=MS.gene023309
chr4.2 exon 15741591 15743171 15741591 ID=MS.gene023309.t1.exon1;Parent=MS.gene023309.t1
chr4.2 CDS 15741591 15743171 15741591 ID=cds.MS.gene023309.t1;Parent=MS.gene023309.t1
Gene Sequence

>MS.gene023309

ATGAGTAGTGGAAGTGATAAATCAGGTGTTTGTTGGGAAGACGACGGTGGTGGTATGGATGAACTCTTAGCTGTAGTTGGTTACAAAGTGAAATCTTCAGACATGGCTGAAGTTGCTCAAAAACTTGAACAACTTGAACAAGCTATGATGGGTAATAATTTCCATGACCATGATGAATCCACCATCGCTCAACATCTTTCAAACGACACCGTTCATTACAATCCCGCTGATATCTCTAACTGGCTTCAAACTATGCTTTCCAATTTTGACCCTCAACCTAATATCCCTTCCGTTAACTCCGACGATAACGACCTTAACGCCATTCCTGGTAAAGCCATCTACGCCGTCAACACTCCGTTAGAAGATTCTCTTCCTTGTCGGAAAAGAATCAAAAGAAATGAATCCGTTACCGTTACTACTGAGTCAACAACTCGTCCTGTAATGGTGGTGGAGACACAAGAGAAAGGGATTCGTCTTGTTCATAGTTTGATGGCTTGTGCTGAAGCTGTTGAACAGAATAATCTAACGGTTGCCGAGGCTTTGGTGAAACAGATCGGATACTTAGCGGTATCACAAGAAGGTGCTATGAGAAAAGTTGCTACTTATTTTGCTGAAGGTTTAGCAAGAAGAATCTACGGTGTGTTTCCACAGCATTCCGTTTCCGATTCGCTTCAGATCCATTTCTATGAAACTTGTCCTAATTTAAAATTCGCTCACTTCACTGCGAATCAAGCTATTCTAGAAGCTTTTCAAGGAAAATCTCGTGTTCATGTCATTGATTTTTCGATTAATCAAGGGATGCAGTGGCCGGCGCTTATGCAGGCGCTTGCTTTACGTCCTGGTGGTCCTCCTGCTTTTCGGTTGACCGGAATCGGACCTCCTGCGTCGGATAACTCCGATCATCTTCAACAAGTGGGTTGGAGGCTTGCTCAGTTTGCACAAACGATTCATGTTCAGTTTGAGTATCGTGGTTTTGTGGCTAATAGTTTAGCTGATCTTGATGCTTCGATGCTTGAACTCCGGTCACCGGAAACGGAATCTGTTGCGGTTAACTCTGTTTTTGAGCTTCATAAACTTAACGCGCGTCCCGGCGCGTTGGAGAAAGTGTTTTCTGTCATTCGTCAGATTCGGCCGGAGATTGTCACCGTTGTTGAGCAAGAAGCAAATCACAATGGACCGGCTTTTCTAGACCGGTTCACCGAGTCACTTCACTATTACTCAACTCTATTTGACTCGCTGGAGGGTTCATCAGTTGAACCGCAAGATAAAGCTATGTCGGAGGTTTATCTAGGGAAGCAAATCTGCAACGTGGTGGCGTGTGAAGGAACGGACCGGGTTGAACGTCATGAGACACTGAACCAGTGGAGGAACCGGTTTAATTCAGCCGGATTCTCTCCGGTTCATTTGGGATCTAACGCGTTCAAACAAGCGAGTATGTTGTTAGCACTTTTCGCCGGTGGGGATGGTTACAAGGTGGAAGAAAATGATGGGTGTCTTATGCTTGGTTGGCACACTAGACCTTTGATTGCAACTTCAGCTTGGAAACTCGCCGCTGCTAACTCAGGGGTGGTTTCACACTGA
Protein sequence

>MS.gene023309.t1

MSSGSDKSGVCWEDDGGGMDELLAVVGYKVKSSDMAEVAQKLEQLEQAMMGNNFHDHDESTIAQHLSNDTVHYNPADISNWLQTMLSNFDPQPNIPSVNSDDNDLNAIPGKAIYAVNTPLEDSLPCRKRIKRNESVTVTTESTTRPVMVVETQEKGIRLVHSLMACAEAVEQNNLTVAEALVKQIGYLAVSQEGAMRKVATYFAEGLARRIYGVFPQHSVSDSLQIHFYETCPNLKFAHFTANQAILEAFQGKSRVHVIDFSINQGMQWPALMQALALRPGGPPAFRLTGIGPPASDNSDHLQQVGWRLAQFAQTIHVQFEYRGFVANSLADLDASMLELRSPETESVAVNSVFELHKLNARPGALEKVFSVIRQIRPEIVTVVEQEANHNGPAFLDRFTESLHYYSTLFDSLEGSSVEPQDKAMSEVYLGKQICNVVACEGTDRVERHETLNQWRNRFNSAGFSPVHLGSNAFKQASMLLALFAGGDGYKVEENDGCLMLGWHTRPLIATSAWKLAAANSGVVSH