AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023424

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023424.t1 MTR_8g080670 91.463 410 35 0 1 410 1 410 0.0 712
MS.gene023424.t1 MTR_8g080680 61.187 438 106 3 1 422 1 390 2.07e-178 504
MS.gene023424.t1 MTR_8g080690 61.139 404 113 6 1 388 1 376 4.46e-141 408
MS.gene023424.t1 MTR_3g085180 50.331 453 188 12 1 435 1 434 2.88e-114 342
MS.gene023424.t1 MTR_1g053715 37.175 269 129 7 123 365 17 271 5.91e-35 133
MS.gene023424.t1 MTR_8g463140 63.158 76 28 0 159 234 62 137 4.88e-29 112
MS.gene023424.t1 MTR_7g092930 65.789 76 26 0 159 234 65 140 2.40e-28 114
MS.gene023424.t1 MTR_4g088555 58.140 86 34 1 150 235 53 136 3.56e-27 106
MS.gene023424.t1 MTR_2g078770 54.945 91 40 1 144 234 41 130 4.54e-27 106
MS.gene023424.t1 MTR_8g005960 44.521 146 70 4 94 234 120 259 5.40e-27 110
MS.gene023424.t1 MTR_7g444860 45.038 131 52 2 116 234 99 221 1.13e-25 106
MS.gene023424.t1 MTR_5g046670 58.667 75 31 0 160 234 242 316 1.62e-24 105
MS.gene023424.t1 MTR_4g109770 39.490 157 71 5 99 234 42 195 3.85e-24 104
MS.gene023424.t1 MTR_2g014200 57.333 75 32 0 160 234 62 136 8.20e-24 97.1
MS.gene023424.t1 MTR_7g028740 51.765 85 41 0 150 234 65 149 3.66e-23 100
MS.gene023424.t1 MTR_3g099080 45.872 109 57 1 162 270 98 204 9.21e-23 99.8
MS.gene023424.t1 MTR_2g461920 41.406 128 59 3 122 234 117 243 1.36e-22 100
MS.gene023424.t1 MTR_1g035010 45.872 109 51 2 127 234 75 176 2.26e-21 97.4
MS.gene023424.t1 MTR_2g046550 46.218 119 56 2 127 241 104 218 2.99e-21 97.4
MS.gene023424.t1 MTR_8g096780 44.737 114 60 1 162 275 99 209 3.10e-21 95.5
MS.gene023424.t1 MTR_1g086250 56.757 74 32 0 161 234 146 219 1.20e-20 95.5
MS.gene023424.t1 MTR_7g110320 38.125 160 87 4 127 286 113 260 2.93e-20 94.0
MS.gene023424.t1 MTR_7g110320 38.125 160 87 4 127 286 113 260 3.82e-20 94.0
MS.gene023424.t1 MTR_2g020620 32.934 167 95 5 169 329 97 252 1.05e-15 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023424.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.04e-37 141
MS.gene023424.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.04e-37 141
MS.gene023424.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.04e-37 141
MS.gene023424.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.04e-37 141
MS.gene023424.t1 AT5G43270 34.253 435 195 21 1 393 1 386 1.70e-37 141
MS.gene023424.t1 AT5G43270 34.253 435 195 21 1 393 1 386 1.70e-37 141
MS.gene023424.t1 AT5G43270 34.253 435 195 21 1 393 1 386 1.70e-37 141
MS.gene023424.t1 AT1G27370 41.633 245 114 7 12 234 11 248 7.32e-37 138
MS.gene023424.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.25e-36 138
MS.gene023424.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.25e-36 138
MS.gene023424.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.25e-36 138
MS.gene023424.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.25e-36 138
MS.gene023424.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.25e-36 138
MS.gene023424.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.25e-36 138
MS.gene023424.t1 AT2G42200 45.517 145 56 4 160 304 72 193 1.08e-28 116
MS.gene023424.t1 AT3G57920 49.541 109 48 2 129 234 27 131 2.13e-27 112
MS.gene023424.t1 AT1G02065 50.000 114 51 1 127 234 147 260 4.13e-27 111
MS.gene023424.t1 AT1G69170 62.069 87 30 2 150 234 111 196 6.68e-27 112
MS.gene023424.t1 AT2G33810 58.667 75 31 0 161 235 53 127 8.39e-25 99.4
MS.gene023424.t1 AT3G15270 56.757 74 32 0 161 234 62 135 2.91e-23 96.7
MS.gene023424.t1 AT1G53160 58.108 74 31 0 161 234 53 126 2.48e-22 94.0
MS.gene023424.t1 AT1G53160 58.108 74 31 0 161 234 53 126 2.48e-22 94.0
MS.gene023424.t1 AT1G53160 58.108 74 31 0 161 234 53 126 2.81e-22 93.6
MS.gene023424.t1 AT5G50670 54.667 75 34 0 160 234 99 173 3.58e-22 97.8
MS.gene023424.t1 AT5G50670 54.667 75 34 0 160 234 99 173 3.58e-22 97.8
MS.gene023424.t1 AT5G50570 54.667 75 34 0 160 234 99 173 3.58e-22 97.8
MS.gene023424.t1 AT5G50570 54.667 75 34 0 160 234 99 173 3.58e-22 97.8
MS.gene023424.t1 AT3G60030 57.534 73 31 0 162 234 127 199 2.66e-21 97.4
MS.gene023424.t1 AT2G47070 48.958 96 42 1 139 234 90 178 1.02e-20 95.5
MS.gene023424.t1 AT1G20980 56.000 75 33 0 160 234 118 192 4.40e-20 93.6
MS.gene023424.t1 AT1G76580 56.000 75 33 0 160 234 112 186 1.85e-19 91.7
MS.gene023424.t1 AT1G76580 56.000 75 33 0 160 234 112 186 2.07e-19 91.3
MS.gene023424.t1 AT1G76580 56.000 75 33 0 160 234 112 186 3.54e-19 90.9
MS.gene023424.t1 AT1G02065 47.191 89 41 1 127 209 147 235 2.18e-17 81.6
MS.gene023424.t1 AT5G18830 46.575 73 39 0 162 234 138 210 6.37e-16 80.9
MS.gene023424.t1 AT5G18830 46.575 73 39 0 162 234 138 210 6.53e-16 80.9
MS.gene023424.t1 AT5G18830 46.575 73 39 0 162 234 138 210 6.94e-16 80.5
MS.gene023424.t1 AT2G47070 60.417 48 19 0 187 234 2 49 1.73e-11 66.6

Find 78 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCTGTTTGAGTTTGA+TGG 0.225842 4.2:+14069375 MS.gene023424:CDS
CATTGATCCTCTTGGTTTCA+AGG 0.239743 4.2:-14070488 None:intergenic
CAAGGACTTTGGATGTAAAA+TGG 0.260990 4.2:-14070470 None:intergenic
TCAATCTAAAACTCGGTAAA+AGG 0.273664 4.2:+14068928 MS.gene023424:CDS
ACTTTCAAATGCAAATTTAC+TGG 0.299044 4.2:-14068805 None:intergenic
TATAACTACCATAGGAGATT+TGG 0.321384 4.2:-14069111 None:intergenic
GCTTCAATAGGCCCTTCTTT+AGG 0.325945 4.2:-14068861 None:intergenic
CCTCTTGGTTTCAAGGACTT+TGG 0.327846 4.2:-14070481 None:intergenic
AGCTTTACCTGCTACATTGC+TGG 0.356873 4.2:-14069157 None:intergenic
ACAAAGATGATTGACTTGAT+TGG 0.359832 4.2:-14069470 None:intergenic
CTCTAGCCTCATCAGAATAT+TGG 0.364549 4.2:+14070912 MS.gene023424:CDS
GTGCCAATGTTGTCGTTTAT+AGG 0.365991 4.2:-14070408 None:intergenic
TATGGTAGTTATAGATGGTT+TGG 0.373519 4.2:+14069121 MS.gene023424:CDS
CTCCCTCTTCACGGTGGGAA+TGG 0.375697 4.2:+14068568 MS.gene023424:CDS
GACATTCATGTTAGTCCAAC+TGG 0.375986 4.2:-14071051 None:intergenic
GCAGTCTTTGAATAAGCAAA+TGG 0.377299 4.2:-14070318 None:intergenic
CAAAGATGATTGACTTGATT+GGG 0.382950 4.2:-14069469 None:intergenic
CCGCCTCTCTGCCAGGTTGA+AGG 0.385535 4.2:+14069023 MS.gene023424:CDS
TGCTATGGCTTGAGCGGTAC+TGG 0.386033 4.2:-14070867 None:intergenic
TCTCCTATGGTAGTTATAGA+TGG 0.387501 4.2:+14069116 MS.gene023424:CDS
CTGACCTGCATTGATCCTCT+TGG 0.395116 4.2:-14070496 None:intergenic
CTCTGCCAGGTTGAAGGTTG+TGG 0.395224 4.2:+14069029 MS.gene023424:CDS
CCAACTGGAAGTGGTTATTC+TGG 0.417567 4.2:+14068699 MS.gene023424:CDS
TCCCTCTTCACGGTGGGAAT+GGG 0.429201 4.2:+14068569 MS.gene023424:CDS
CATCAAACTCAAACAGATCA+TGG 0.444519 4.2:-14069374 None:intergenic
GGTGCATTAGAAGCTTCAAT+AGG 0.450824 4.2:-14068873 None:intergenic
ATGTTCTAGAGAAACGGATT+TGG 0.456583 4.2:-14070831 None:intergenic
AAAATTGAATTACCTAAAGA+AGG 0.460103 4.2:+14068849 MS.gene023424:CDS
ATCTAAATGTTCTAGAGAAA+CGG 0.467117 4.2:-14070837 None:intergenic
TGGTTGTTCGATACGCCAAC+TGG 0.470221 4.2:+14068684 MS.gene023424:CDS
CAATCAGTTCATCACCAACT+AGG 0.472722 4.2:+14068769 MS.gene023424:CDS
TTCGATACGCCAACTGGAAG+TGG 0.476010 4.2:+14068690 MS.gene023424:CDS
ACATTGTAAGTTCTGAGCAT+TGG 0.495722 4.2:-14069000 None:intergenic
AATTCGATACTCCAGTTTGG+TGG 0.496264 4.2:-14068642 None:intergenic
GACGCGTCATCTTTAGTTGT+TGG 0.503063 4.2:+14068963 MS.gene023424:CDS
ACAATGTCCGCCTCTCTGCC+AGG 0.504313 4.2:+14069016 MS.gene023424:CDS
ATGCAAAATCTCCCTCTTCA+CGG 0.507595 4.2:+14068559 MS.gene023424:CDS
TCTAATTCGATACTCCAGTT+TGG 0.509220 4.2:-14068645 None:intergenic
TGACAGAAGAGAGGAAGCAC+AGG 0.512878 4.2:-14070783 None:intergenic
CAGTCTTTGAATAAGCAAAT+GGG 0.517662 4.2:-14070317 None:intergenic
GATAGCATAGTTACAGTACT+TGG 0.519974 4.2:-14070432 None:intergenic
CTCTTCTGTCAACCAGTCCG+TGG 0.520942 4.2:+14070795 MS.gene023424:CDS
TTTACCATTGGAATCATCAT+GGG 0.526394 4.2:-14068829 None:intergenic
CGTCATCTTTAGTTGTTGGA+AGG 0.527554 4.2:+14068967 MS.gene023424:CDS
AGATCATTCCAAATCTCCTA+TGG 0.535644 4.2:+14069103 MS.gene023424:CDS
TTGTGGCCTTGACCTTTCAT+CGG 0.541799 4.2:+14069046 MS.gene023424:CDS
CGCATATGAATCCCACGGAC+TGG 0.545835 4.2:-14070807 None:intergenic
TAGACCGAGAAAATAATGTC+TGG 0.549417 4.2:+14068664 MS.gene023424:CDS
AATCAGTTCATCACCAACTA+GGG 0.553568 4.2:+14068770 MS.gene023424:CDS
TCCCATTCCCACCGTGAAGA+GGG 0.557718 4.2:-14068570 None:intergenic
AAACCATCTATAACTACCAT+AGG 0.563385 4.2:-14069119 None:intergenic
CTGACTCGTTGTGTGTGCTA+TGG 0.564583 4.2:-14070882 None:intergenic
ACTGGTGAAAACACGACTCA+AGG 0.568330 4.2:-14071033 None:intergenic
TGAGTTTGAGATCCACATGC+TGG 0.569545 4.2:-14070963 None:intergenic
ATCCCATTCCCACCGTGAAG+AGG 0.571348 4.2:-14068571 None:intergenic
TCTTCTGTCAACCAGTCCGT+GGG 0.576912 4.2:+14070796 MS.gene023424:CDS
AAATTGAATTACCTAAAGAA+GGG 0.590524 4.2:+14068850 MS.gene023424:CDS
CAAGGCCACAACCTTCAACC+TGG 0.591428 4.2:-14069034 None:intergenic
AAAATCTCCCTCTTCACGGT+GGG 0.595195 4.2:+14068563 MS.gene023424:CDS
CGGTAAAAGGATGAAATTCG+AGG 0.600138 4.2:+14068941 MS.gene023424:CDS
CTAGATACACAAGAAAGCAT+TGG 0.604314 4.2:-14068894 None:intergenic
CCATTGCTCAATCTAAAACT+CGG 0.604403 4.2:+14068921 MS.gene023424:CDS
AAGCCTATAAACGACAACAT+TGG 0.614145 4.2:+14070405 MS.gene023424:CDS
GAAACCAAGAGGATCAATGC+AGG 0.614595 4.2:+14070492 MS.gene023424:CDS
AGTTCCCATGATGATTCCAA+TGG 0.616986 4.2:+14068825 MS.gene023424:CDS
CCAGAATAACCACTTCCAGT+TGG 0.626070 4.2:-14068699 None:intergenic
CAATATTCTGATGAGGCTAG+AGG 0.629859 4.2:-14070911 None:intergenic
AATATGTCGATTCCAGCATG+TGG 0.630965 4.2:+14070951 MS.gene023424:CDS
CAACCTTCAACCTGGCAGAG+AGG 0.635133 4.2:-14069026 None:intergenic
CCAAAGTCCTTGAAACCAAG+AGG 0.636381 4.2:+14070481 MS.gene023424:CDS
TTTGGATGTAAAATGGACAT+CGG 0.653646 4.2:-14070463 None:intergenic
CGGACTGGTTGACAGAAGAG+AGG 0.658311 4.2:-14070792 None:intergenic
ACTGTGCCAATATTCTGATG+AGG 0.658353 4.2:-14070918 None:intergenic
CAAAATCTCCCTCTTCACGG+TGG 0.658631 4.2:+14068562 MS.gene023424:CDS
CCTTCAACCTGGCAGAGAGG+CGG 0.660903 4.2:-14069023 None:intergenic
TCTTTGTCTTCGTCACCCTG+TGG 0.667989 4.2:+14069486 MS.gene023424:CDS
TTGGTCGCATATGAATCCCA+CGG 0.682290 4.2:-14070812 None:intergenic
TGTGTGTGCTATGGCTTGAG+CGG 0.704952 4.2:-14070873 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAACATAATCTATTTATTT+TGG + chr4.2:14070586-14070605 MS.gene023424:intron 10.0%
!! AAACATAAATGAACTAAAAT+TGG - chr4.2:14070189-14070208 None:intergenic 15.0%
!!! AGGAAATAATAGTATTATTT+TGG + chr4.2:14069218-14069237 MS.gene023424:intron 15.0%
!!! ATTAAACTTGCTTTTTTTTT+TGG + chr4.2:14069295-14069314 MS.gene023424:intron 15.0%
!! AAAATTGAATTACCTAAAGA+AGG + chr4.2:14068849-14068868 MS.gene023424:CDS 20.0%
!! AAATTGAATTACCTAAAGAA+GGG + chr4.2:14068850-14068869 MS.gene023424:CDS 20.0%
!! TGTAATTGATTAGAGTAATA+TGG + chr4.2:14070646-14070665 MS.gene023424:intron 20.0%
!!! AGTTGAATTTTTATTACATG+AGG - chr4.2:14070131-14070150 None:intergenic 20.0%
!!! ATTTATTTCATGGATCTTTT+TGG + chr4.2:14069827-14069846 MS.gene023424:intron 20.0%
!!! CAGTTTTACAATAATGATTA+CGG - chr4.2:14069449-14069468 None:intergenic 20.0%
!!! GTCATTTTTCATTTATTTCA+TGG + chr4.2:14069817-14069836 MS.gene023424:intron 20.0%
!!! TTTTAACTTCTTGTAAATAC+TGG + chr4.2:14069795-14069814 MS.gene023424:intron 20.0%
! AAACTTAGTAGAAAATCATG+AGG - chr4.2:14070546-14070565 None:intergenic 25.0%
! AATCAAATAACAAGCAGAAA+CGG - chr4.2:14069547-14069566 None:intergenic 25.0%
! ACTTTCAAATGCAAATTTAC+TGG - chr4.2:14068808-14068827 None:intergenic 25.0%
! AGAAAAATAAGTATTACCAC+AGG - chr4.2:14069505-14069524 None:intergenic 25.0%
! GAAAAATAAGTATTACCACA+GGG - chr4.2:14069504-14069523 None:intergenic 25.0%
! GATTAAGCATCTTTATCTTT+AGG - chr4.2:14069872-14069891 None:intergenic 25.0%
! GATTAGAAGATAAAAGAGAA+TGG - chr4.2:14070262-14070281 None:intergenic 25.0%
!! AGGTAATTCAATTTTACCAT+TGG - chr4.2:14068844-14068863 None:intergenic 25.0%
!! ATTTTCAGTAGCTTTTGTAT+CGG - chr4.2:14068610-14068629 None:intergenic 25.0%
!! TCAAGAGAAAATCTTTTGTT+TGG - chr4.2:14070386-14070405 None:intergenic 25.0%
!!! AACTTGCTTTTTTTTTTGGA+TGG + chr4.2:14069299-14069318 MS.gene023424:intron 25.0%
!!! AAGAGAAAATCTTTTGTTTG+GGG - chr4.2:14070384-14070403 None:intergenic 25.0%
!!! ATCTAAATGTTCTAGAGAAA+CGG - chr4.2:14070840-14070859 None:intergenic 25.0%
!!! CAAGAGAAAATCTTTTGTTT+GGG - chr4.2:14070385-14070404 None:intergenic 25.0%
!!! TAATTTTTGGTTCTACATCA+GGG + chr4.2:14068730-14068749 MS.gene023424:CDS 25.0%
!!! TGTATAGTGGATTTACTTAA+AGG + chr4.2:14070014-14070033 MS.gene023424:intron 25.0%
!!! TTCTGCTTGTTATTTGATTT+AGG + chr4.2:14069548-14069567 MS.gene023424:intron 25.0%
AAAAATTACAACCACCAAAC+TGG + chr4.2:14068631-14068650 MS.gene023424:CDS 30.0%
AAACAGTTATCGTGATTGTT+TGG + chr4.2:14070044-14070063 MS.gene023424:intron 30.0%
AAACCATCTATAACTACCAT+AGG - chr4.2:14069122-14069141 None:intergenic 30.0%
AAATGAATAACACTGTGCAA+GGG - chr4.2:14070693-14070712 None:intergenic 30.0%
AACTAAAATTGGCTACTCTT+CGG - chr4.2:14070178-14070197 None:intergenic 30.0%
ATAATAACCATGCAGAGTAA+TGG + chr4.2:14069947-14069966 MS.gene023424:intron 30.0%
CAGTCTTTGAATAAGCAAAT+GGG - chr4.2:14070320-14070339 None:intergenic 30.0%
TACCTATAAAGTTAGTAAGC+AGG - chr4.2:14069706-14069725 None:intergenic 30.0%
TATAACTACCATAGGAGATT+TGG - chr4.2:14069114-14069133 None:intergenic 30.0%
TATGGTAGTTATAGATGGTT+TGG + chr4.2:14069121-14069140 MS.gene023424:CDS 30.0%
TCATATTAGAGTTGCTAGAA+GGG + chr4.2:14069970-14069989 MS.gene023424:intron 30.0%
TGATGTTCCTAAAACTAGAT+TGG + chr4.2:14070082-14070101 MS.gene023424:intron 30.0%
TGTTTGGATCTAATTGACAT+AGG + chr4.2:14069198-14069217 MS.gene023424:intron 30.0%
TTAACAACGGTATGATTGTT+TGG + chr4.2:14069182-14069201 MS.gene023424:intron 30.0%
TTACTCTAATCAATTACACC+TGG - chr4.2:14070644-14070663 None:intergenic 30.0%
TTATCTTATTCGTGTCACAA+AGG - chr4.2:14069894-14069913 None:intergenic 30.0%
TTCCTGCTTACTAACTTTAT+AGG + chr4.2:14069701-14069720 MS.gene023424:intron 30.0%
TTTACCATTGGAATCATCAT+GGG - chr4.2:14068832-14068851 None:intergenic 30.0%
TTTGGATGTAAAATGGACAT+CGG - chr4.2:14070466-14070485 None:intergenic 30.0%
! AATAATGTTTTGCCGATGAA+AGG - chr4.2:14069061-14069080 None:intergenic 30.0%
! ACAACCAGACATTATTTTCT+CGG - chr4.2:14068671-14068690 None:intergenic 30.0%
! TCAATCTAAAACTCGGTAAA+AGG + chr4.2:14068928-14068947 MS.gene023424:CDS 30.0%
! TTTTACCATTGGAATCATCA+TGG - chr4.2:14068833-14068852 None:intergenic 30.0%
! TTTTCAGTAGCTTTTGTATC+GGG - chr4.2:14068609-14068628 None:intergenic 30.0%
!! ACAAAGATGATTGACTTGAT+TGG - chr4.2:14069473-14069492 None:intergenic 30.0%
!! AGAGAAAATCTTTTGTTTGG+GGG - chr4.2:14070383-14070402 None:intergenic 30.0%
!! CAAAGATGATTGACTTGATT+GGG - chr4.2:14069472-14069491 None:intergenic 30.0%
!! TTGATTTAGGTTGTTAAACC+TGG + chr4.2:14069561-14069580 MS.gene023424:intron 30.0%
!!! CTAATTTTTGGTTCTACATC+AGG + chr4.2:14068729-14068748 MS.gene023424:CDS 30.0%
!!! GACTTCTAGATTTTTTTTCC+AGG - chr4.2:14069582-14069601 None:intergenic 30.0%
AAATCTTCTACACCTGCAAA+AGG - chr4.2:14070725-14070744 None:intergenic 35.0%
AAGCAGAAACGGATATAAAC+TGG - chr4.2:14069536-14069555 None:intergenic 35.0%
AAGCCTATAAACGACAACAT+TGG + chr4.2:14070405-14070424 MS.gene023424:CDS 35.0%
AATATGACCATTACTCTGCA+TGG - chr4.2:14069957-14069976 None:intergenic 35.0%
AATCAGTTCATCACCAACTA+GGG + chr4.2:14068770-14068789 MS.gene023424:CDS 35.0%
AGATCATTCCAAATCTCCTA+TGG + chr4.2:14069103-14069122 MS.gene023424:CDS 35.0%
CAAATGAATAACACTGTGCA+AGG - chr4.2:14070694-14070713 None:intergenic 35.0%
CAAGGACTTTGGATGTAAAA+TGG - chr4.2:14070473-14070492 None:intergenic 35.0%
CACTTTGCTTAAAATGCTGA+AGG + chr4.2:14069349-14069368 MS.gene023424:intron 35.0%
CAGGTAAAGCTTCTTAACAA+CGG + chr4.2:14069169-14069188 MS.gene023424:intron 35.0%
CATCAAACTCAAACAGATCA+TGG - chr4.2:14069377-14069396 None:intergenic 35.0%
GATAGCATAGTTACAGTACT+TGG - chr4.2:14070435-14070454 None:intergenic 35.0%
GCAGTCTTTGAATAAGCAAA+TGG - chr4.2:14070321-14070340 None:intergenic 35.0%
GTCATATTAGAGTTGCTAGA+AGG + chr4.2:14069969-14069988 MS.gene023424:intron 35.0%
TAGACCGAGAAAATAATGTC+TGG + chr4.2:14068664-14068683 MS.gene023424:CDS 35.0%
TCTAATTCGATACTCCAGTT+TGG - chr4.2:14068648-14068667 None:intergenic 35.0%
TCTCCTATGGTAGTTATAGA+TGG + chr4.2:14069116-14069135 MS.gene023424:CDS 35.0%
! AGTGTCTATTGTAACTTGCA+AGG - chr4.2:14069730-14069749 None:intergenic 35.0%
! CATGATCTGTTTGAGTTTGA+TGG + chr4.2:14069375-14069394 MS.gene023424:CDS 35.0%
! CCATTGCTCAATCTAAAACT+CGG + chr4.2:14068921-14068940 MS.gene023424:CDS 35.0%
! CTAGATACACAAGAAAGCAT+TGG - chr4.2:14068897-14068916 None:intergenic 35.0%
! CTTGCACAGTGTTATTCATT+TGG + chr4.2:14070692-14070711 MS.gene023424:intron 35.0%
! GTTTTGCTAATATCCCTAGT+TGG - chr4.2:14068786-14068805 None:intergenic 35.0%
! TTTGGTAGAATACCTTTTGC+AGG + chr4.2:14070710-14070729 MS.gene023424:intron 35.0%
!! ACATTGTAAGTTCTGAGCAT+TGG - chr4.2:14069003-14069022 None:intergenic 35.0%
!! ATGTTCTAGAGAAACGGATT+TGG - chr4.2:14070834-14070853 None:intergenic 35.0%
!! GCTGTTTTGTATGTTTTGCA+AGG - chr4.2:14070355-14070374 None:intergenic 35.0%
!!! ACTCACACCAATCTAGTTTT+AGG - chr4.2:14070092-14070111 None:intergenic 35.0%
AATTCGATACTCCAGTTTGG+TGG - chr4.2:14068645-14068664 None:intergenic 40.0%
ACTGTGCCAATATTCTGATG+AGG - chr4.2:14070921-14070940 None:intergenic 40.0%
AGTTCCCATGATGATTCCAA+TGG + chr4.2:14068825-14068844 MS.gene023424:CDS 40.0%
ATGCAAAATCTCCCTCTTCA+CGG + chr4.2:14068559-14068578 MS.gene023424:CDS 40.0%
CAATATTCTGATGAGGCTAG+AGG - chr4.2:14070914-14070933 None:intergenic 40.0%
CAATCAGTTCATCACCAACT+AGG + chr4.2:14068769-14068788 MS.gene023424:CDS 40.0%
CATGGTGGTAGCAATCAATA+TGG - chr4.2:14070612-14070631 None:intergenic 40.0%
CATTGATCCTCTTGGTTTCA+AGG - chr4.2:14070491-14070510 None:intergenic 40.0%
CGGTAAAAGGATGAAATTCG+AGG + chr4.2:14068941-14068960 MS.gene023424:CDS 40.0%
CGTCATCTTTAGTTGTTGGA+AGG + chr4.2:14068967-14068986 MS.gene023424:CDS 40.0%
CTCTAGCCTCATCAGAATAT+TGG + chr4.2:14070912-14070931 MS.gene023424:CDS 40.0%
GGTGCATTAGAAGCTTCAAT+AGG - chr4.2:14068876-14068895 None:intergenic 40.0%
TACACCTGGCAATATTTGCA+TGG - chr4.2:14070630-14070649 None:intergenic 40.0%
! AGGGATTGCTATTAGAGTTG+TGG + chr4.2:14069989-14070008 MS.gene023424:intron 40.0%
! GGGATTGCTATTAGAGTTGT+GGG + chr4.2:14069990-14070009 MS.gene023424:intron 40.0%
! GTAGCTTTTGTATCGGGTAA+TGG - chr4.2:14068603-14068622 None:intergenic 40.0%
! GTGCCAATGTTGTCGTTTAT+AGG - chr4.2:14070411-14070430 None:intergenic 40.0%
!! AATATGTCGATTCCAGCATG+TGG + chr4.2:14070951-14070970 MS.gene023424:CDS 40.0%
!! CTTTTGTATCGGGTAATGGT+AGG - chr4.2:14068599-14068618 None:intergenic 40.0%
!!! CCGAGTTTTAGATTGAGCAA+TGG - chr4.2:14068924-14068943 None:intergenic 40.0%
!!! TCTGGCTCTGAGCTAATTTT+TGG + chr4.2:14068717-14068736 MS.gene023424:CDS 40.0%
AAAATCTCCCTCTTCACGGT+GGG + chr4.2:14068563-14068582 MS.gene023424:CDS 45.0%
ACCACCATGCAAATATTGCC+AGG + chr4.2:14070623-14070642 MS.gene023424:intron 45.0%
ACCTGGCAATATTTGCATGG+TGG - chr4.2:14070627-14070646 None:intergenic 45.0%
ACTGGTGAAAACACGACTCA+AGG - chr4.2:14071036-14071055 None:intergenic 45.0%
AGCTTTACCTGCTACATTGC+TGG - chr4.2:14069160-14069179 None:intergenic 45.0%
CAACTATAGCCTGCATAGCA+TGG - chr4.2:14069610-14069629 None:intergenic 45.0%
CCAAAGTCCTTGAAACCAAG+AGG + chr4.2:14070481-14070500 MS.gene023424:CDS 45.0%
CCAACTGGAAGTGGTTATTC+TGG + chr4.2:14068699-14068718 MS.gene023424:CDS 45.0%
CCAGAATAACCACTTCCAGT+TGG - chr4.2:14068702-14068721 None:intergenic 45.0%
CCTCTTGGTTTCAAGGACTT+TGG - chr4.2:14070484-14070503 None:intergenic 45.0%
GAAACCAAGAGGATCAATGC+AGG + chr4.2:14070492-14070511 MS.gene023424:CDS 45.0%
GACGCGTCATCTTTAGTTGT+TGG + chr4.2:14068963-14068982 MS.gene023424:CDS 45.0%
GCTTCAATAGGCCCTTCTTT+AGG - chr4.2:14068864-14068883 None:intergenic 45.0%
TTGGTCGCATATGAATCCCA+CGG - chr4.2:14070815-14070834 None:intergenic 45.0%
! AGTCTAAGACCATGCTATGC+AGG + chr4.2:14069598-14069617 MS.gene023424:intron 45.0%
! GATTTTGCCAGCAATGTAGC+AGG + chr4.2:14069150-14069169 MS.gene023424:CDS 45.0%
! GTTTTGCCGATGAAAGGTCA+AGG - chr4.2:14069055-14069074 None:intergenic 45.0%
! TAGAGTTGTGGGCTGTATAG+TGG + chr4.2:14070001-14070020 MS.gene023424:intron 45.0%
! TGAGTCGTGTTTTCACCAGT+TGG + chr4.2:14071036-14071055 MS.gene023424:CDS 45.0%
! TGAGTTTGAGATCCACATGC+TGG - chr4.2:14070966-14070985 None:intergenic 45.0%
! TTGTGGCCTTGACCTTTCAT+CGG + chr4.2:14069046-14069065 MS.gene023424:CDS 45.0%
CAAAATCTCCCTCTTCACGG+TGG + chr4.2:14068562-14068581 MS.gene023424:CDS 50.0%
TCTTCTGTCAACCAGTCCGT+GGG + chr4.2:14070796-14070815 MS.gene023424:CDS 50.0%
TCTTTGTCTTCGTCACCCTG+TGG + chr4.2:14069486-14069505 MS.gene023424:CDS 50.0%
TGGTTGTTCGATACGCCAAC+TGG + chr4.2:14068684-14068703 MS.gene023424:CDS 50.0%
TGTGTGTGCTATGGCTTGAG+CGG - chr4.2:14070876-14070895 None:intergenic 50.0%
TTCGATACGCCAACTGGAAG+TGG + chr4.2:14068690-14068709 MS.gene023424:CDS 50.0%
! CTGACCTGCATTGATCCTCT+TGG - chr4.2:14070499-14070518 None:intergenic 50.0%
! CTGACTCGTTGTGTGTGCTA+TGG - chr4.2:14070885-14070904 None:intergenic 50.0%
! TGACAGAAGAGAGGAAGCAC+AGG - chr4.2:14070786-14070805 None:intergenic 50.0%
ATCCCATTCCCACCGTGAAG+AGG - chr4.2:14068574-14068593 None:intergenic 55.0%
CAACCTTCAACCTGGCAGAG+AGG - chr4.2:14069029-14069048 None:intergenic 55.0%
CAAGGCCACAACCTTCAACC+TGG - chr4.2:14069037-14069056 None:intergenic 55.0%
CGCATATGAATCCCACGGAC+TGG - chr4.2:14070810-14070829 None:intergenic 55.0%
CTCTTCTGTCAACCAGTCCG+TGG + chr4.2:14070795-14070814 MS.gene023424:CDS 55.0%
TCCCATTCCCACCGTGAAGA+GGG - chr4.2:14068573-14068592 None:intergenic 55.0%
TCCCTCTTCACGGTGGGAAT+GGG + chr4.2:14068569-14068588 MS.gene023424:CDS 55.0%
TGCTATGGCTTGAGCGGTAC+TGG - chr4.2:14070870-14070889 None:intergenic 55.0%
! CTCTGCCAGGTTGAAGGTTG+TGG + chr4.2:14069029-14069048 MS.gene023424:CDS 55.0%
!! CGGACTGGTTGACAGAAGAG+AGG - chr4.2:14070795-14070814 None:intergenic 55.0%
ACAATGTCCGCCTCTCTGCC+AGG + chr4.2:14069016-14069035 MS.gene023424:CDS 60.0%
CCTTCAACCTGGCAGAGAGG+CGG - chr4.2:14069026-14069045 None:intergenic 60.0%
CTCCCTCTTCACGGTGGGAA+TGG + chr4.2:14068568-14068587 MS.gene023424:CDS 60.0%
! CCGCCTCTCTGCCAGGTTGA+AGG + chr4.2:14069023-14069042 MS.gene023424:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 14068549 14071063 14068549 ID=MS.gene023424
chr4.2 mRNA 14068549 14071063 14068549 ID=MS.gene023424.t1;Parent=MS.gene023424
chr4.2 exon 14068549 14069171 14068549 ID=MS.gene023424.t1.exon1;Parent=MS.gene023424.t1
chr4.2 CDS 14068549 14069171 14068549 ID=cds.MS.gene023424.t1;Parent=MS.gene023424.t1
chr4.2 exon 14069371 14069507 14069371 ID=MS.gene023424.t1.exon2;Parent=MS.gene023424.t1
chr4.2 CDS 14069371 14069507 14069371 ID=cds.MS.gene023424.t1;Parent=MS.gene023424.t1
chr4.2 exon 14070298 14070513 14070298 ID=MS.gene023424.t1.exon3;Parent=MS.gene023424.t1
chr4.2 CDS 14070298 14070513 14070298 ID=cds.MS.gene023424.t1;Parent=MS.gene023424.t1
chr4.2 exon 14070732 14071063 14070732 ID=MS.gene023424.t1.exon4;Parent=MS.gene023424.t1
chr4.2 CDS 14070732 14071063 14070732 ID=cds.MS.gene023424.t1;Parent=MS.gene023424.t1
Gene Sequence

>MS.gene023424

ATGGATTTGGATGCAAAATCTCCCTCTTCACGGTGGGAATGGGATAGCCTACCATTACCCGATACAAAAGCTACTGAAAATAAAAAATTACAACCACCAAACTGGAGTATCGAATTAGACCGAGAAAATAATGTCTGGTTGTTCGATACGCCAACTGGAAGTGGTTATTCTGGCTCTGAGCTAATTTTTGGTTCTACATCAGGGAGTTCGAAATCAGATTCAATCAGTTCATCACCAACTAGGGATATTAGCAAAACCAGTAAATTTGCATTTGAAAGTTCCCATGATGATTCCAATGGTAAAATTGAATTACCTAAAGAAGGGCCTATTGAAGCTTCTAATGCACCAATGCTTTCTTGTGTATCTAGTGAACCATTGCTCAATCTAAAACTCGGTAAAAGGATGAAATTCGAGGACGCGTCATCTTTAGTTGTTGGAAGGAAATGTAAGTCCAATGCTCAGAACTTACAATGTCCGCCTCTCTGCCAGGTTGAAGGTTGTGGCCTTGACCTTTCATCGGCAAAACATTATTATCGCAAACGAAGAGTTTGTGTAGATCATTCCAAATCTCCTATGGTAGTTATAGATGGTTTGGAACGACGATTTTGCCAGCAATGTAGCAGGTAAAGCTTCTTAACAACGGTATGATTGTTTGGATCTAATTGACATAGGAAATAATAGTATTATTTTGGAATGATGAATTCTTGCTAGCACATCATTAGAAGTTAATATTACTACTCAATCTGATTAAACTTGCTTTTTTTTTTGGATGGTGTTGAATAAATTGCTCTAGAATTGATCACTTTGCTTAAAATGCTGAAGGTTCCATGATCTGTTTGAGTTTGATGGAAAGAAGAAAAGCTGCAGACGACGTCTTTCACAACACAATGCATTGCGCCGTAATCATTATTGTAAAACTGCCCAATCAAGTCAATCATCTTTGTCTTCGTCACCCTGTGGTAATACTTATTTTTCTTTATTTTTCCAGTTTATATCCGTTTCTGCTTGTTATTTGATTTAGGTTGTTAAACCTGGAAAAAAAATCTAGAAGTCTAAGACCATGCTATGCAGGCTATAGTTGAGTATTAACATTGCTGAATGAAGATTGTTTGAAAAACTTAAATACTTGACATCACTTTTCTTTTCTTTGCTTTCCTGCTTACTAACTTTATAGGTAACCTTGCAAGTTACAATAGACACTAATGTCATGTGACAAAACTTCTTAATTTTCTACAATTTTCTATCTTTTTAACTTCTTGTAAATACTGGTCATTTTTCATTTATTTCATGGATCTTTTTGGATTTTCAATTGTCATACATCCTAAAGATAAAGATGCTTAATCCTTTGTGACACGAATAAGATAAAGCATGTTCAAGAGTTCTAGCAGTATCAATTTCATAATAACCATGCAGAGTAATGGTCATATTAGAGTTGCTAGAAGGGATTGCTATTAGAGTTGTGGGCTGTATAGTGGATTTACTTAAAGGCACAAGAAAACAGTTATCGTGATTGTTTGGACTTGAACGCGAGTATGATGTTCCTAAAACTAGATTGGTGTGAGTATGAAATTCATTAAAACCTCATGTAATAAAAATTCAACTTCAAGCTGTTAGTCAAAACTTATACCGAAGAGTAGCCAATTTTAGTTCATTTATGTTTATTATAGTTACTTTCAAATCATTCATTTCACGATAATGATGCAAATTTTACCATTCTCTTTTATCTTCTAATCTATTTTAGTTCTGCAGATGAGAAGCAACAGATGAACCCATTTGCTTATTCAAAGACTGCTATAAATTTAGCCTTGCAAAACATACAAAACAGCGAGCTCCCCCAAACAAAAGATTTTCTCTTGAAGCCTATAAACGACAACATTGGCACACCAAGTACTGTAACTATGCTATCTGATGATTCCGATGTCCATTTTACATCCAAAGTCCTTGAAACCAAGAGGATCAATGCAGGTCAGTTCACGTCTTACTTTAAAATTAAGCCTCATGATTTTCTACTAAGTTTAGATTGTTACATTTATCATGTAAACATAATCTATTTATTTTGGCCATATTGATTGCTACCACCATGCAAATATTGCCAGGTGTAATTGATTAGAGTAATATGGTATTGAAGTATGTAATAATATCCCTTGCACAGTGTTATTCATTTGGTAGAATACCTTTTGCAGGTGTAGAAGATTTCATTAGCTTCTCTGATACAGATGCTACACAAGACTTCACCTGTGCTTCCTCTCTTCTGTCAACCAGTCCGTGGGATTCATATGCGACCAAATCCGTTTCTCTAGAACATTTAGATTGTACAACCAGTACCGCTCAAGCCATAGCACACACAACGAGTCAGTGTATGCCTCTAGCCTCATCAGAATATTGGCACAGTGTTCTTGATCAATATGTCGATTCCAGCATGTGGATCTCAAACTCAGACTGTGAAGAAAGCGATCATTTTCAAGAATTTCAATGTTCTGAGAACCTTGAGTCGTGTTTTCACCAGTTGGACTAA
Protein sequence

>MS.gene023424.t1

MDLDAKSPSSRWEWDSLPLPDTKATENKKLQPPNWSIELDRENNVWLFDTPTGSGYSGSELIFGSTSGSSKSDSISSSPTRDISKTSKFAFESSHDDSNGKIELPKEGPIEASNAPMLSCVSSEPLLNLKLGKRMKFEDASSLVVGRKCKSNAQNLQCPPLCQVEGCGLDLSSAKHYYRKRRVCVDHSKSPMVVIDGLERRFCQQCSRFHDLFEFDGKKKSCRRRLSQHNALRRNHYCKTAQSSQSSLSSSPCDEKQQMNPFAYSKTAINLALQNIQNSELPQTKDFLLKPINDNIGTPSTVTMLSDDSDVHFTSKVLETKRINAGVEDFISFSDTDATQDFTCASSLLSTSPWDSYATKSVSLEHLDCTTSTAQAIAHTTSQCMPLASSEYWHSVLDQYVDSSMWISNSDCEESDHFQEFQCSENLESCFHQLD