AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023425

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023425.t1 MTR_8g080680 89.975 399 38 1 1 399 1 397 0.0 736
MS.gene023425.t1 MTR_8g080670 63.318 428 93 3 1 382 1 410 1.05e-171 486
MS.gene023425.t1 MTR_8g080690 67.454 381 98 5 1 360 1 376 1.19e-170 482
MS.gene023425.t1 MTR_3g085180 46.712 441 183 8 1 402 1 428 6.60e-111 332
MS.gene023425.t1 MTR_1g053715 33.645 321 169 11 80 385 18 309 2.18e-34 130
MS.gene023425.t1 MTR_7g092930 35.798 257 136 6 85 332 17 253 5.69e-34 129
MS.gene023425.t1 MTR_7g444860 43.312 157 72 4 50 206 82 221 1.18e-30 120
MS.gene023425.t1 MTR_3g099080 45.113 133 71 1 113 245 77 207 2.60e-30 120
MS.gene023425.t1 MTR_8g463140 63.158 76 28 0 131 206 62 137 1.54e-29 113
MS.gene023425.t1 MTR_4g088555 67.105 76 25 0 131 206 60 135 2.32e-29 111
MS.gene023425.t1 MTR_5g046670 42.400 125 71 1 82 206 193 316 8.00e-28 115
MS.gene023425.t1 MTR_8g005960 49.194 124 52 3 83 206 147 259 1.02e-27 112
MS.gene023425.t1 MTR_4g109770 56.977 86 35 1 134 219 123 206 3.64e-27 112
MS.gene023425.t1 MTR_2g078770 60.000 75 30 0 132 206 56 130 6.32e-27 105
MS.gene023425.t1 MTR_8g096780 34.404 218 108 4 134 323 99 309 1.65e-26 110
MS.gene023425.t1 MTR_2g461920 61.644 73 28 0 134 206 171 243 9.69e-26 109
MS.gene023425.t1 MTR_2g014200 57.895 76 32 0 131 206 61 136 1.81e-25 101
MS.gene023425.t1 MTR_7g028740 57.534 73 31 0 134 206 77 149 8.74e-25 104
MS.gene023425.t1 MTR_1g035010 47.573 103 52 1 106 206 74 176 1.04e-23 104
MS.gene023425.t1 MTR_1g086250 56.250 80 35 0 134 213 147 226 1.14e-23 103
MS.gene023425.t1 MTR_7g110320 49.000 100 51 0 119 218 130 229 2.68e-23 102
MS.gene023425.t1 MTR_2g046550 57.895 76 32 0 134 209 139 214 2.70e-23 102
MS.gene023425.t1 MTR_7g110320 49.000 100 51 0 119 218 130 229 4.11e-23 102
MS.gene023425.t1 MTR_2g020620 34.524 168 91 5 141 301 97 252 1.48e-17 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023425.t1 AT1G27370 40.000 265 144 8 77 332 119 377 5.44e-45 160
MS.gene023425.t1 AT1G27370 40.000 265 144 8 77 332 119 377 5.44e-45 160
MS.gene023425.t1 AT1G27370 40.000 265 144 8 77 332 119 377 5.44e-45 160
MS.gene023425.t1 AT1G27370 40.000 265 144 8 77 332 119 377 5.44e-45 160
MS.gene023425.t1 AT1G27370 40.000 265 144 8 77 332 119 377 5.44e-45 160
MS.gene023425.t1 AT1G27370 40.000 265 144 8 77 332 119 377 5.44e-45 160
MS.gene023425.t1 AT1G27370 58.779 131 52 2 77 206 119 248 1.50e-43 155
MS.gene023425.t1 AT5G43270 61.194 134 50 2 74 206 109 241 2.86e-42 154
MS.gene023425.t1 AT5G43270 61.194 134 50 2 74 206 109 241 2.86e-42 154
MS.gene023425.t1 AT5G43270 61.194 134 50 2 74 206 109 241 2.86e-42 154
MS.gene023425.t1 AT1G27360 33.245 376 193 11 1 324 1 370 2.10e-41 150
MS.gene023425.t1 AT1G27360 33.245 376 193 11 1 324 1 370 2.10e-41 150
MS.gene023425.t1 AT1G27360 33.245 376 193 11 1 324 1 370 2.10e-41 150
MS.gene023425.t1 AT1G27360 33.245 376 193 11 1 324 1 370 2.10e-41 150
MS.gene023425.t1 AT2G42200 32.271 251 140 7 132 366 72 308 4.32e-31 122
MS.gene023425.t1 AT3G57920 31.210 314 164 13 85 388 27 298 3.02e-29 117
MS.gene023425.t1 AT1G69170 65.333 75 26 0 132 206 122 196 3.26e-29 117
MS.gene023425.t1 AT1G02065 46.923 130 59 1 83 212 147 266 2.69e-28 114
MS.gene023425.t1 AT2G33810 56.667 90 37 1 117 206 39 126 3.31e-27 105
MS.gene023425.t1 AT1G53160 61.644 73 28 0 134 206 54 126 2.23e-25 102
MS.gene023425.t1 AT1G53160 61.644 73 28 0 134 206 54 126 2.76e-25 101
MS.gene023425.t1 AT1G53160 61.644 73 28 0 134 206 54 126 2.76e-25 101
MS.gene023425.t1 AT3G15270 57.534 73 31 0 134 206 63 135 2.56e-24 99.4
MS.gene023425.t1 AT1G76580 53.933 89 41 0 132 220 112 200 3.35e-24 105
MS.gene023425.t1 AT1G76580 53.933 89 41 0 132 220 112 200 3.46e-24 105
MS.gene023425.t1 AT1G76580 53.933 89 41 0 132 220 112 200 4.98e-24 105
MS.gene023425.t1 AT5G50670 51.807 83 40 0 124 206 91 173 7.42e-24 102
MS.gene023425.t1 AT5G50670 51.807 83 40 0 124 206 91 173 7.42e-24 102
MS.gene023425.t1 AT5G50570 51.807 83 40 0 124 206 91 173 7.42e-24 102
MS.gene023425.t1 AT5G50570 51.807 83 40 0 124 206 91 173 7.42e-24 102
MS.gene023425.t1 AT3G60030 58.904 73 30 0 134 206 127 199 1.17e-23 103
MS.gene023425.t1 AT2G47070 60.274 73 29 0 134 206 106 178 1.52e-23 103
MS.gene023425.t1 AT1G20980 58.667 75 31 0 132 206 118 192 6.02e-23 102
MS.gene023425.t1 AT5G18830 42.727 110 53 2 97 206 111 210 3.81e-19 90.1
MS.gene023425.t1 AT5G18830 42.727 110 53 2 97 206 111 210 4.49e-19 90.1
MS.gene023425.t1 AT5G18830 42.727 110 53 2 97 206 111 210 4.54e-19 90.1
MS.gene023425.t1 AT1G02065 43.434 99 46 2 83 181 147 235 2.15e-17 81.6
MS.gene023425.t1 AT2G47070 58.333 48 20 0 159 206 2 49 1.10e-11 67.0

Find 76 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTTGGTGGTTGTAATTT+TGG 0.078496 4.2:-14063875 None:intergenic
TTTGGATGTAAAATGGAAAT+TGG 0.200557 4.2:-14065552 None:intergenic
CATGATCTGTTTGAGTTTGA+TGG 0.225842 4.2:+14064520 MS.gene023425:CDS
CCAAACCTTTCTTGTGTATC+TGG 0.244468 4.2:+14064001 MS.gene023425:CDS
GAAGGACTTTGGATGTAAAA+TGG 0.246221 4.2:-14065559 None:intergenic
CCCGGTGGAGGTGGTTCTTC+TGG 0.267974 4.2:+14063944 MS.gene023425:CDS
CTTGACCGAGAAATCAATTT+CGG 0.268342 4.2:+14063908 MS.gene023425:CDS
TTCAAAGAACCTTATGAGTA+TGG 0.284972 4.2:+14065944 MS.gene023425:CDS
TCAATCTAAGACTTGGTAAA+AGG 0.294020 4.2:+14064035 MS.gene023425:CDS
TCTGGCTCTGAGTTAATTCA+TGG 0.303523 4.2:+14063962 MS.gene023425:CDS
TATAACCACCATGGGAGATT+TGG 0.333181 4.2:-14064266 None:intergenic
ATAAAGCTGATTGGCTTGAT+TGG 0.333859 4.2:-14064615 None:intergenic
ATTAGAATGTTCTTCAGAAA+TGG 0.343450 4.2:-14065762 None:intergenic
ACAACCACCAAACTGGAGTA+TGG 0.348781 4.2:+14063883 MS.gene023425:CDS
CAAAATTACAACCACCAAAC+TGG 0.349918 4.2:+14063876 MS.gene023425:CDS
GCAGTCTTGGAATTAGAAAA+TGG 0.356442 4.2:-14065407 None:intergenic
AGCTTTACCTGCTACATTGC+TGG 0.356873 4.2:-14064312 None:intergenic
TCTCCCATGGTGGTTATAGA+TGG 0.357918 4.2:+14064271 MS.gene023425:CDS
CATGGTGGTTATAGATGGTT+TGG 0.360015 4.2:+14064276 MS.gene023425:CDS
TTGACCGAGAAATCAATTTC+GGG 0.364920 4.2:+14063909 MS.gene023425:CDS
ACAACCCGAAATTGATTTCT+CGG 0.376943 4.2:-14063913 None:intergenic
CTGATTCATTGCATGAGTTA+TGG 0.392149 4.2:-14065807 None:intergenic
GGCTAAATTTGTAGCAGTCT+TGG 0.413824 4.2:-14065420 None:intergenic
ACTCTTGGTTGGAAGGACTT+TGG 0.430559 4.2:-14065570 None:intergenic
TTCACAGTTGGAGTTGGAAT+GGG 0.437116 4.2:+14063814 MS.gene023425:CDS
CATCAAACTCAAACAGATCA+TGG 0.444519 4.2:-14064519 None:intergenic
CAGCTTGACGATGATATATA+CGG 0.462985 4.2:-14064591 None:intergenic
CTTCACAGTTGGAGTTGGAA+TGG 0.465688 4.2:+14063813 MS.gene023425:CDS
CCACCACGCTGCCAGGTTGA+AGG 0.471978 4.2:+14064178 MS.gene023425:CDS
CGCATATGAATCCCACTGAT+TGG 0.473627 4.2:-14065732 None:intergenic
TCCCTCTTCACAGTTGGAGT+TGG 0.476978 4.2:+14063808 MS.gene023425:CDS
CCAGATACACAAGAAAGGTT+TGG 0.481067 4.2:-14064001 None:intergenic
CTCTTGCTTCATCAGAGCCA+TGG 0.481784 4.2:+14065837 MS.gene023425:CDS
GTGAATAAGATAAAGCTGAT+TGG 0.481792 4.2:-14064624 None:intergenic
TCAAGTTCCATACTCCAGTT+TGG 0.484691 4.2:-14063890 None:intergenic
GCCAGAAGAACCACCTCCAC+CGG 0.484703 4.2:-14063945 None:intergenic
AACATTCATGTTAACCCAGC+TGG 0.488020 4.2:-14065973 None:intergenic
GATTGATACTCTTGGTTGGA+AGG 0.496148 4.2:-14065577 None:intergenic
TGGTGAAAACCATACTCATA+AGG 0.504888 4.2:-14065953 None:intergenic
AGTTCCATACTCCAGTTTGG+TGG 0.506654 4.2:-14063887 None:intergenic
ACAATGTCCACCACGCTGCC+AGG 0.516200 4.2:+14064171 MS.gene023425:CDS
TATGATACACCCGGTGGAGG+TGG 0.516299 4.2:+14063935 MS.gene023425:CDS
ACATTGTAAGTTCTGACCAT+TGG 0.517425 4.2:-14064155 None:intergenic
GCTTTATCTTATTCACGCAA+TGG 0.532317 4.2:+14064631 MS.gene023425:CDS
GAAAGCATATTGACAGTACT+TGG 0.532864 4.2:-14065521 None:intergenic
ATAACCTGGATTGATACTCT+TGG 0.533141 4.2:-14065585 None:intergenic
GATACACAAGAAAGGTTTGG+TGG 0.536044 4.2:-14063998 None:intergenic
CCTGGATTGATACTCTTGGT+TGG 0.537469 4.2:-14065581 None:intergenic
TCCAACTCCAACTGTGAAGA+GGG 0.543018 4.2:-14063809 None:intergenic
AACTTTCAAATCACAATGCA+AGG 0.545524 4.2:+14064566 MS.gene023425:CDS
AGTTATGGCTTGAAAAGCAC+TGG 0.554669 4.2:-14065792 None:intergenic
CCATTGCTCAATCTAAGACT+TGG 0.554906 4.2:+14064028 MS.gene023425:CDS
GTTCACCAGATACACAAGAA+AGG 0.558590 4.2:-14064006 None:intergenic
TTGTATGATACACCCGGTGG+AGG 0.575298 4.2:+14063932 MS.gene023425:CDS
CCAACCAAGAGTATCAATCC+AGG 0.578039 4.2:+14065581 MS.gene023425:CDS
GAAAAGAAATGCAAGTCCAA+TGG 0.587403 4.2:+14064139 MS.gene023425:CDS
CCAAGTCTTAGATTGAGCAA+TGG 0.590002 4.2:-14064028 None:intergenic
CAAGAGCACAACCTTCAACC+TGG 0.591018 4.2:-14064189 None:intergenic
CCAGAAGAACCACCTCCACC+GGG 0.592307 4.2:-14063944 None:intergenic
AATATGTCAATTCCAACATG+TGG 0.594822 4.2:+14065876 MS.gene023425:CDS
CTCTTCTGTCAACCAATCAG+TGG 0.597609 4.2:+14065720 MS.gene023425:CDS
TCTTCTGTCAACCAATCAGT+GGG 0.601400 4.2:+14065721 MS.gene023425:CDS
TTCCAACTCCAACTGTGAAG+AGG 0.601663 4.2:-14063810 None:intergenic
AATATCTCCCTCTTCACAGT+TGG 0.605827 4.2:+14063802 MS.gene023425:CDS
AGACTGCTACAAATTTAGCC+TGG 0.607461 4.2:+14065423 MS.gene023425:CDS
AAACCATCTATAACCACCAT+GGG 0.608088 4.2:-14064274 None:intergenic
CCTTCAACCTGGCAGCGTGG+TGG 0.616962 4.2:-14064178 None:intergenic
AGAGTTTGAGATCCACATGT+TGG 0.619666 4.2:-14065888 None:intergenic
TTCGGGTTGTATGATACACC+CGG 0.621273 4.2:+14063926 MS.gene023425:CDS
CAAACCATCTATAACCACCA+TGG 0.633030 4.2:-14064275 None:intergenic
AAGTCATGCCAAATCTCCCA+TGG 0.638950 4.2:+14064258 MS.gene023425:CDS
CAACCTTCAACCTGGCAGCG+TGG 0.648763 4.2:-14064181 None:intergenic
CATGGCTCTGATGAAGCAAG+AGG 0.663007 4.2:-14065836 None:intergenic
TGATCATGATCACTACACCA+TGG 0.714032 4.2:-14065854 None:intergenic
TCATGCCAAATCTCCCATGG+TGG 0.722301 4.2:+14064261 MS.gene023425:CDS
GGGTTGTATGATACACCCGG+TGG 0.728686 4.2:+14063929 MS.gene023425:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTTCATAGATCTTAT+TGG + chr4.2:14064977-14064996 MS.gene023425:intron 15.0%
!!! TTCTATCTTCTAATATATTT+TGG + chr4.2:14065356-14065375 MS.gene023425:intron 15.0%
!! AATCTAGTTAGTAAATACTT+CGG - chr4.2:14065170-14065189 None:intergenic 20.0%
!!! CAAAAATCTTTCTTTTTCAA+GGG + chr4.2:14064093-14064112 MS.gene023425:CDS 20.0%
!!! TAGATAGTTGATTTAGTTAA+AGG + chr4.2:14065057-14065076 MS.gene023425:intron 20.0%
! AAAAAATCAGCACAAAAATG+TGG - chr4.2:14065624-14065643 None:intergenic 25.0%
! ATGAAATGTAGAAATCTAAG+AGG + chr4.2:14064733-14064752 MS.gene023425:intron 25.0%
! ATTAGAATGTTCTTCAGAAA+TGG - chr4.2:14065765-14065784 None:intergenic 25.0%
! TTTGGATGTAAAATGGAAAT+TGG - chr4.2:14065555-14065574 None:intergenic 25.0%
!! CCTTGAAAAAGAAAGATTTT+TGG - chr4.2:14064095-14064114 None:intergenic 25.0%
!! CTTTTGTATTAAGTAATGGT+AGG - chr4.2:14063844-14063863 None:intergenic 25.0%
!!! AGTTGTATTTTTACTAAACC+AGG - chr4.2:14065218-14065237 None:intergenic 25.0%
!!! ATGATTAAACTTTTGAGTTG+TGG + chr4.2:14065032-14065051 MS.gene023425:intron 25.0%
!!! CCAAAAATCTTTCTTTTTCA+AGG + chr4.2:14064092-14064111 MS.gene023425:CDS 25.0%
!!! GTTGCTTTTGTATTAAGTAA+TGG - chr4.2:14063848-14063867 None:intergenic 25.0%
!!! TGATTTTTTTTTACTCTTGC+AGG + chr4.2:14065635-14065654 MS.gene023425:intron 25.0%
AAACTCTTGAGTAAATAAGC+AGG - chr4.2:14064856-14064875 None:intergenic 30.0%
AAATGAACAGAACTTACAAG+AGG - chr4.2:14064954-14064973 None:intergenic 30.0%
AACTTTCAAATCACAATGCA+AGG + chr4.2:14064566-14064585 MS.gene023425:CDS 30.0%
AATATGTCAATTCCAACATG+TGG + chr4.2:14065876-14065895 MS.gene023425:CDS 30.0%
CTTACAAGAGGTTAAAAGAT+AGG - chr4.2:14064942-14064961 None:intergenic 30.0%
GCAATGTTAATAGTCAACTA+AGG - chr4.2:14064779-14064798 None:intergenic 30.0%
GTGAATAAGATAAAGCTGAT+TGG - chr4.2:14064627-14064646 None:intergenic 30.0%
TTATCCTGATTGTTAGAGTT+TGG + chr4.2:14065116-14065135 MS.gene023425:intron 30.0%
TTCAAAGAACCTTATGAGTA+TGG + chr4.2:14065944-14065963 MS.gene023425:CDS 30.0%
! ATTTTCAATCGTCATACATC+TGG + chr4.2:14065000-14065019 MS.gene023425:intron 30.0%
! CACTTTTCTTAAAATGCTGA+AGG + chr4.2:14064494-14064513 MS.gene023425:intron 30.0%
! TCAATCTAAGACTTGGTAAA+AGG + chr4.2:14064035-14064054 MS.gene023425:CDS 30.0%
! TGGTAAAAGGATGTATTTTG+AGG + chr4.2:14064048-14064067 MS.gene023425:CDS 30.0%
! TTTGCGATGATAATCTTTTG+CGG - chr4.2:14064224-14064243 None:intergenic 30.0%
!! AATCTAATTCTACTTGCTGT+TGG + chr4.2:14064444-14064463 MS.gene023425:intron 30.0%
!! AGTTTGGTGGTTGTAATTTT+GGG - chr4.2:14063877-14063896 None:intergenic 30.0%
!!! AGATTTTTGGAATCACTTTC+TGG - chr4.2:14064082-14064101 None:intergenic 30.0%
AAACCATCTATAACCACCAT+GGG - chr4.2:14064277-14064296 None:intergenic 35.0%
AACCAAAAGTGGATGTTCTT+TGG - chr4.2:14065268-14065287 None:intergenic 35.0%
ACCTATGAAACAACCAAAAG+TGG - chr4.2:14065279-14065298 None:intergenic 35.0%
ATAACCTGGATTGATACTCT+TGG - chr4.2:14065588-14065607 None:intergenic 35.0%
ATGTCCAAACTCTAACAATC+AGG - chr4.2:14065123-14065142 None:intergenic 35.0%
CAAAATTACAACCACCAAAC+TGG + chr4.2:14063876-14063895 MS.gene023425:CDS 35.0%
CAGCTTGACGATGATATATA+CGG - chr4.2:14064594-14064613 None:intergenic 35.0%
CATCAAACTCAAACAGATCA+TGG - chr4.2:14064522-14064541 None:intergenic 35.0%
CTAAACCAGGTATTAACGAA+TGG - chr4.2:14065205-14065224 None:intergenic 35.0%
CTGATTCATTGCATGAGTTA+TGG - chr4.2:14065810-14065829 None:intergenic 35.0%
CTTGACCGAGAAATCAATTT+CGG + chr4.2:14063908-14063927 MS.gene023425:CDS 35.0%
GAAAAGAAATGCAAGTCCAA+TGG + chr4.2:14064139-14064158 MS.gene023425:CDS 35.0%
GAAGGACTTTGGATGTAAAA+TGG - chr4.2:14065562-14065581 None:intergenic 35.0%
GCAATAAACGTGAAATAACC+TGG - chr4.2:14065602-14065621 None:intergenic 35.0%
GCAGTCTTGGAATTAGAAAA+TGG - chr4.2:14065410-14065429 None:intergenic 35.0%
GCTTTATCTTATTCACGCAA+TGG + chr4.2:14064631-14064650 MS.gene023425:CDS 35.0%
GTTAGTAAATACTTCGGCTT+AGG - chr4.2:14065164-14065183 None:intergenic 35.0%
TGGTGAAAACCATACTCATA+AGG - chr4.2:14065956-14065975 None:intergenic 35.0%
TTGACCGAGAAATCAATTTC+GGG + chr4.2:14063909-14063928 MS.gene023425:CDS 35.0%
! AAGGGAAAATCTTTTGCTTG+GGG - chr4.2:14065473-14065492 None:intergenic 35.0%
! ACAACCCGAAATTGATTTCT+CGG - chr4.2:14063916-14063935 None:intergenic 35.0%
! ACATTGTAAGTTCTGACCAT+TGG - chr4.2:14064158-14064177 None:intergenic 35.0%
! CAAGGGAAAATCTTTTGCTT+GGG - chr4.2:14065474-14065493 None:intergenic 35.0%
! CATGATCTGTTTGAGTTTGA+TGG + chr4.2:14064520-14064539 MS.gene023425:CDS 35.0%
! GAAAGCATATTGACAGTACT+TGG - chr4.2:14065524-14065543 None:intergenic 35.0%
! TCAAGGGAAAATCTTTTGCT+TGG - chr4.2:14065475-14065494 None:intergenic 35.0%
!! ATAAAGCTGATTGGCTTGAT+TGG - chr4.2:14064618-14064637 None:intergenic 35.0%
!! CAGTTTGGTGGTTGTAATTT+TGG - chr4.2:14063878-14063897 None:intergenic 35.0%
!! TCCACTTTTGGTTGTTTCAT+AGG + chr4.2:14065275-14065294 MS.gene023425:intron 35.0%
AATATCTCCCTCTTCACAGT+TGG + chr4.2:14063802-14063821 MS.gene023425:CDS 40.0%
AGACTGCTACAAATTTAGCC+TGG + chr4.2:14065423-14065442 MS.gene023425:CDS 40.0%
CAAACCATCTATAACCACCA+TGG - chr4.2:14064278-14064297 None:intergenic 40.0%
CAACTAAGGCATGCATAACA+TGG - chr4.2:14064765-14064784 None:intergenic 40.0%
CATGGTGGTTATAGATGGTT+TGG + chr4.2:14064276-14064295 MS.gene023425:CDS 40.0%
CCAAACCTTTCTTGTGTATC+TGG + chr4.2:14064001-14064020 MS.gene023425:CDS 40.0%
CCAAGTCTTAGATTGAGCAA+TGG - chr4.2:14064031-14064050 None:intergenic 40.0%
CCAGATACACAAGAAAGGTT+TGG - chr4.2:14064004-14064023 None:intergenic 40.0%
GATACACAAGAAAGGTTTGG+TGG - chr4.2:14064001-14064020 None:intergenic 40.0%
GATTGATACTCTTGGTTGGA+AGG - chr4.2:14065580-14065599 None:intergenic 40.0%
GGCTAAATTTGTAGCAGTCT+TGG - chr4.2:14065423-14065442 None:intergenic 40.0%
GTATGCCATTCGTTAATACC+TGG + chr4.2:14065197-14065216 MS.gene023425:intron 40.0%
GTTCACCAGATACACAAGAA+AGG - chr4.2:14064009-14064028 None:intergenic 40.0%
TATAACCACCATGGGAGATT+TGG - chr4.2:14064269-14064288 None:intergenic 40.0%
TCAAGTTCCATACTCCAGTT+TGG - chr4.2:14063893-14063912 None:intergenic 40.0%
TCAATCGTCATACATCTGGA+TGG + chr4.2:14065004-14065023 MS.gene023425:intron 40.0%
TCTTCTGTCAACCAATCAGT+GGG + chr4.2:14065721-14065740 MS.gene023425:CDS 40.0%
TGATCATGATCACTACACCA+TGG - chr4.2:14065857-14065876 None:intergenic 40.0%
TTCACAGTTGGAGTTGGAAT+GGG + chr4.2:14063814-14063833 MS.gene023425:CDS 40.0%
! AATGTCTCGTGTAACTTGCA+AGG - chr4.2:14064880-14064899 None:intergenic 40.0%
! AGAGTTTGAGATCCACATGT+TGG - chr4.2:14065891-14065910 None:intergenic 40.0%
! AGGGAAAATCTTTTGCTTGG+GGG - chr4.2:14065472-14065491 None:intergenic 40.0%
! AGTTATGGCTTGAAAAGCAC+TGG - chr4.2:14065795-14065814 None:intergenic 40.0%
! CCATTGCTCAATCTAAGACT+TGG + chr4.2:14064028-14064047 MS.gene023425:CDS 40.0%
! GACCAAAGAACATCCACTTT+TGG + chr4.2:14065263-14065282 MS.gene023425:intron 40.0%
! GTGTCAATGTTGTCTTTTGC+AGG - chr4.2:14065500-14065519 None:intergenic 40.0%
! TTGTCTTTTGCAGGCTTCAA+GGG - chr4.2:14065491-14065510 None:intergenic 40.0%
!! TCTGGCTCTGAGTTAATTCA+TGG + chr4.2:14063962-14063981 MS.gene023425:CDS 40.0%
AAGTCATGCCAAATCTCCCA+TGG + chr4.2:14064258-14064277 MS.gene023425:CDS 45.0%
ACAACCACCAAACTGGAGTA+TGG + chr4.2:14063883-14063902 MS.gene023425:CDS 45.0%
ACTCTTGGTTGGAAGGACTT+TGG - chr4.2:14065573-14065592 None:intergenic 45.0%
AGCTTTACCTGCTACATTGC+TGG - chr4.2:14064315-14064334 None:intergenic 45.0%
AGTTCCATACTCCAGTTTGG+TGG - chr4.2:14063890-14063909 None:intergenic 45.0%
CCTGGATTGATACTCTTGGT+TGG - chr4.2:14065584-14065603 None:intergenic 45.0%
CGCATATGAATCCCACTGAT+TGG - chr4.2:14065735-14065754 None:intergenic 45.0%
CTCTTCTGTCAACCAATCAG+TGG + chr4.2:14065720-14065739 MS.gene023425:CDS 45.0%
CTTCACAGTTGGAGTTGGAA+TGG + chr4.2:14063813-14063832 MS.gene023425:CDS 45.0%
TCCAACTCCAACTGTGAAGA+GGG - chr4.2:14063812-14063831 None:intergenic 45.0%
TCTCCCATGGTGGTTATAGA+TGG + chr4.2:14064271-14064290 MS.gene023425:CDS 45.0%
TTCCAACTCCAACTGTGAAG+AGG - chr4.2:14063813-14063832 None:intergenic 45.0%
! GAGTATGGTTTTCACCAGCT+GGG + chr4.2:14065959-14065978 MS.gene023425:CDS 45.0%
! GATTTTGCCAGCAATGTAGC+AGG + chr4.2:14064305-14064324 MS.gene023425:CDS 45.0%
! GTTGTCTTTTGCAGGCTTCA+AGG - chr4.2:14065492-14065511 None:intergenic 45.0%
! TGAGTATGGTTTTCACCAGC+TGG + chr4.2:14065958-14065977 MS.gene023425:CDS 45.0%
!! CCAACCAAGAGTATCAATCC+AGG + chr4.2:14065581-14065600 MS.gene023425:CDS 45.0%
!! TTCGGGTTGTATGATACACC+CGG + chr4.2:14063926-14063945 MS.gene023425:CDS 45.0%
AGCAGGACAGCAAAGAAGAG+TGG - chr4.2:14064839-14064858 None:intergenic 50.0%
CTCTTGCTTCATCAGAGCCA+TGG + chr4.2:14065837-14065856 MS.gene023425:CDS 50.0%
TCATGCCAAATCTCCCATGG+TGG + chr4.2:14064261-14064280 MS.gene023425:CDS 50.0%
TCCCTCTTCACAGTTGGAGT+TGG + chr4.2:14063808-14063827 MS.gene023425:CDS 50.0%
TTGTATGATACACCCGGTGG+AGG + chr4.2:14063932-14063951 MS.gene023425:CDS 50.0%
! CAAGAGCACAACCTTCAACC+TGG - chr4.2:14064192-14064211 None:intergenic 50.0%
! GCTGTTGTGCATGTTTTGCC+AGG - chr4.2:14065444-14065463 None:intergenic 50.0%
!! CATGGCTCTGATGAAGCAAG+AGG - chr4.2:14065839-14065858 None:intergenic 50.0%
GGGTTGTATGATACACCCGG+TGG + chr4.2:14063929-14063948 MS.gene023425:CDS 55.0%
TATGATACACCCGGTGGAGG+TGG + chr4.2:14063935-14063954 MS.gene023425:CDS 55.0%
ACAATGTCCACCACGCTGCC+AGG + chr4.2:14064171-14064190 MS.gene023425:CDS 60.0%
CAACCTTCAACCTGGCAGCG+TGG - chr4.2:14064184-14064203 None:intergenic 60.0%
CCAGAAGAACCACCTCCACC+GGG - chr4.2:14063947-14063966 None:intergenic 60.0%
GCCAGAAGAACCACCTCCAC+CGG - chr4.2:14063948-14063967 None:intergenic 60.0%
CCCGGTGGAGGTGGTTCTTC+TGG + chr4.2:14063944-14063963 MS.gene023425:CDS 65.0%
CCTTCAACCTGGCAGCGTGG+TGG - chr4.2:14064181-14064200 None:intergenic 65.0%
! CCACCACGCTGCCAGGTTGA+AGG + chr4.2:14064178-14064197 MS.gene023425:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 14063788 14065985 14063788 ID=MS.gene023425
chr4.2 mRNA 14063788 14065985 14063788 ID=MS.gene023425.t1;Parent=MS.gene023425
chr4.2 exon 14063788 14064326 14063788 ID=MS.gene023425.t1.exon1;Parent=MS.gene023425.t1
chr4.2 CDS 14063788 14064326 14063788 ID=cds.MS.gene023425.t1;Parent=MS.gene023425.t1
chr4.2 exon 14064516 14064652 14064516 ID=MS.gene023425.t1.exon2;Parent=MS.gene023425.t1
chr4.2 CDS 14064516 14064652 14064516 ID=cds.MS.gene023425.t1;Parent=MS.gene023425.t1
chr4.2 exon 14065387 14065602 14065387 ID=MS.gene023425.t1.exon3;Parent=MS.gene023425.t1
chr4.2 CDS 14065387 14065602 14065387 ID=cds.MS.gene023425.t1;Parent=MS.gene023425.t1
chr4.2 exon 14065657 14065985 14065657 ID=MS.gene023425.t1.exon4;Parent=MS.gene023425.t1
chr4.2 CDS 14065657 14065985 14065657 ID=cds.MS.gene023425.t1;Parent=MS.gene023425.t1
Gene Sequence

>MS.gene023425

ATGGACTTGAATGCAATATCTCCCTCTTCACAGTTGGAGTTGGAATGGGATCACCTACCATTACTTAATACAAAAGCAACTGAAAACCCAAAATTACAACCACCAAACTGGAGTATGGAACTTGACCGAGAAATCAATTTCGGGTTGTATGATACACCCGGTGGAGGTGGTTCTTCTGGCTCTGAGTTAATTCATGGTTCAGCATTTAATCCACCAAACCTTTCTTGTGTATCTGGTGAACCATTGCTCAATCTAAGACTTGGTAAAAGGATGTATTTTGAGGAAGTTTGTCCAGAAAGTGATTCCAAAAATCTTTCTTTTTCAAGGGATCTCATGTCATCTTCAATTCTTGAAAAGAAATGCAAGTCCAATGGTCAGAACTTACAATGTCCACCACGCTGCCAGGTTGAAGGTTGTGCTCTTGATCTTTCATCCGCAAAAGATTATCATCGCAAACGAAGAGTTTGTGAAAGTCATGCCAAATCTCCCATGGTGGTTATAGATGGTTTGGAGCGTCGATTTTGCCAGCAATGTAGCAGGTAAAGCTTCTTAACAACTGTATAATTGTTTAGATCTAATTGACATAGAAAATAATAGTATTTTTTTCGAATGATGAATTCTTGTTAGCACATCATTAAAAGCTAATATTACTACTCAATCTAATTCTACTTGCTGTTGGTGTTGAATAAATTGCTCTAGAATTGATCACTTTTCTTAAAATGCTGAAGGTTCCATGATCTGTTTGAGTTTGATGGAAAGAAGAAAAGCTGCAGACGACAACTTTCAAATCACAATGCAAGGCGCCGTATATATCATCGTCAAGCTGTCCAATCAAGCCAATCAGCTTTATCTTATTCACGCAATGGTAATACTTATTTTTCTCTGATTTTGCAGTTTATCTCAGTTCATGCTTGTTATCTGTTTTTGATTGTTAAAGCTGAAAAAATGAAATGTAGAAATCTAAGAGGCTAAGACCATGTTATGCATGCCTTAGTTGACTATTAACATTGCTGAATGAAAACTGTTTGAAAAATTGAAATGCTTAACACCACTCTTCTTTGCTGTCCTGCTTATTTACTCAAGAGTTTGCCTTGCAAGTTACACGAGACATTAAATGTAATGCAACAGAACTTCTTAATTTTCTACAATTGCCTATCTTTTAACCTCTTGTAAGTTCTGTTCATTTTTCATTTATTTCATAGATCTTATTGGATTTTCAATCGTCATACATCTGGATGGTAAACATGATTAAACTTTTGAGTTGTGGACTAGATAGTTGATTTAGTTAAAGGCATAGCGTACTCTTGATTACAGAATTACACAAATCATTATCCTGATTGTTAGAGTTTGGACATGAGCGCGAGTATAACATTCCTAAGCCGAAGTATTTACTAACTAGATTTGTATGAGTATGCCATTCGTTAATACCTGGTTTAGTAAAAATACAACTTCAAATTGCTGTTAGTCAAAACTTAGACCAAAGAACATCCACTTTTGGTTGTTTCATAGGTTTATTATAGTTAATTGCAAGTCATTCATTTCTCAATGATTCAAATTTTACTGTTGTCTTCTATCTTCTAATATATTTTGGTTCTGCAGATGAGAAGCAACAGATGAGTCCATTTTCTAATTCCAAGACTGCTACAAATTTAGCCTGGCAAAACATGCACAACAGCAAGCTCCCCCAAGCAAAAGATTTTCCCTTGAAGCCTGCAAAAGACAACATTGACACACCAAGTACTGTCAATATGCTTTCTCATGATTCCAATTTCCATTTTACATCCAAAGTCCTTCCAACCAAGAGTATCAATCCAGGTTATTTCACGTTTATTGCCACATTTTTGTGCTGATTTTTTTTTACTCTTGCAGGTGTAGAAGATTTCATGAACTTTTCTGATACAAATGCTACACAAGACTTTACTTGTGCTCTCTCTCTTCTGTCAACCAATCAGTGGGATTCATATGCGACTAAATCCATTTCTGAAGAACATTCTAATCGCTCGACCAGTGCTTTTCAAGCCATAACTCATGCAATGAATCAGCGCATTCCTCTTGCTTCATCAGAGCCATGGTGTAGTGATCATGATCAATATGTCAATTCCAACATGTGGATCTCAAACTCTGCAGATAGCAATCACTTTCAGAAATTTCAACTGTTCAAAGAACCTTATGAGTATGGTTTTCACCAGCTGGGTTAA
Protein sequence

>MS.gene023425.t1

MDLNAISPSSQLELEWDHLPLLNTKATENPKLQPPNWSMELDREINFGLYDTPGGGGSSGSELIHGSAFNPPNLSCVSGEPLLNLRLGKRMYFEEVCPESDSKNLSFSRDLMSSSILEKKCKSNGQNLQCPPRCQVEGCALDLSSAKDYHRKRRVCESHAKSPMVVIDGLERRFCQQCSRFHDLFEFDGKKKSCRRQLSNHNARRRIYHRQAVQSSQSALSYSRNDEKQQMSPFSNSKTATNLAWQNMHNSKLPQAKDFPLKPAKDNIDTPSTVNMLSHDSNFHFTSKVLPTKSINPGVEDFMNFSDTNATQDFTCALSLLSTNQWDSYATKSISEEHSNRSTSAFQAITHAMNQRIPLASSEPWCSDHDQYVNSNMWISNSADSNHFQKFQLFKEPYEYGFHQLG