AlfalfaGEDB | MS.gene023552

M. sativa cultivar XinJiangDaYe / MS.gene023552

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023552.t1	XP_003629740.2	96.5	144	5	0	1	144	1	144	2.50E-65	258.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023552.t1	C0Z2L5	43.5	138	75	2	2	138	10	145	4.1e-16	85.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023552.t1	G7LIP1	96.5	144	5	0	1	144	1	144	1.8e-65	258.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene023552.t1	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023552.t1	MTR_8g086020	96.528	144	5	0	1	144	1	144	2.21e-101	287
MS.gene023552.t1	MTR_4g070860	52.713	129	55	3	15	141	14	138	1.35e-38	128
MS.gene023552.t1	MTR_3g117120	53.509	114	49	1	15	128	14	123	5.02e-35	119
MS.gene023552.t1	MTR_1g022495	55.556	117	47	3	1	117	1	112	6.04e-35	119
MS.gene023552.t1	MTR_3g112220	57.692	78	33	0	33	110	2	79	2.58e-25	92.8
MS.gene023552.t1	MTR_1g087040	40.141	142	74	5	1	140	1	133	7.20e-22	85.5
MS.gene023552.t1	MTR_8g015250	39.000	100	59	1	4	103	45	142	2.09e-15	69.7
MS.gene023552.t1	MTR_1g017570	40.426	94	53	1	26	119	51	141	5.02e-14	65.1
MS.gene023552.t1	MTR_7g029400	37.864	103	57	2	1	103	57	152	5.18e-14	66.2
MS.gene023552.t1	MTR_5g015090	38.393	112	59	4	2	110	32	136	7.52e-14	65.1
MS.gene023552.t1	MTR_4g097440	33.621	116	74	1	2	114	39	154	1.27e-13	64.7
MS.gene023552.t1	MTR_3g109310	31.132	106	73	0	9	114	100	205	1.86e-11	60.5
MS.gene023552.t1	MTR_6g016375	34.000	100	66	0	4	103	59	158	2.54e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023552.t1	AT1G75390	53.623	138	61	2	2	138	10	145	1.11e-43	142
MS.gene023552.t1	AT4G34590	62.222	90	34	0	31	120	23	112	7.99e-35	119
MS.gene023552.t1	AT2G18160	50.000	120	56	3	21	138	15	132	6.33e-32	112
MS.gene023552.t1	AT1G75390	54.082	98	43	1	2	99	10	105	4.87e-30	105
MS.gene023552.t1	AT3G62420	44.444	108	59	1	11	118	2	108	8.14e-26	95.5
MS.gene023552.t1	AT4G37730	38.235	102	52	3	13	103	171	272	1.16e-13	66.6
MS.gene023552.t1	AT5G49450	45.122	82	45	0	33	114	14	95	3.49e-12	60.5
MS.gene023552.t1	AT1G13600	35.455	110	66	2	17	126	70	174	3.72e-12	61.2
MS.gene023552.t1	AT3G30530	39.216	102	61	1	3	103	48	149	4.07e-12	60.8
MS.gene023552.t1	AT5G15830	34.426	122	77	1	13	131	52	173	5.99e-12	60.5
MS.gene023552.t1	AT2G04038	37.931	87	53	1	17	103	57	142	2.78e-11	58.5

Find 23 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCTGAAAATGCTATTCTT+AGG	0.244925	4.3:-14652181	MS.gene023552:CDS
TTGATCTTCGTGCTGATTCT+CGG	0.312597	4.3:+14652321	None:intergenic
GTTCAATGATTGTGGATTCT+TGG	0.317045	4.3:-14652060	MS.gene023552:CDS
TGCTATTCTTAGGGTTCAAA+TGG	0.377563	4.3:-14652171	MS.gene023552:CDS
CCTGGTGGAACTTATTCATC+TGG	0.409672	4.3:-14652440	MS.gene023552:CDS
ATCTGAAAATGCTATTCTTA+GGG	0.417867	4.3:-14652180	MS.gene023552:CDS
GATGAAGAAACAGCAACATA+TGG	0.436530	4.3:-14652297	MS.gene023552:CDS
GATCAATTGTTCAATGATTG+TGG	0.455410	4.3:-14652068	MS.gene023552:CDS
TCTTCGTGCTGATTCTCGGT+TGG	0.456704	4.3:+14652325	None:intergenic
GAGAATCAGCACGAAGATCA+AGG	0.528035	4.3:-14652319	MS.gene023552:CDS
GACCTTGATATGGCTTCTCC+TGG	0.533330	4.3:-14652458	None:intergenic
CTTCAAAACTCTGGATCAGA+GGG	0.550324	4.3:-14652407	MS.gene023552:CDS
ACAAGCTCTCTTCAAAACTC+TGG	0.550443	4.3:-14652416	MS.gene023552:CDS
CTTGATATGGCTTCTCCTGG+TGG	0.579058	4.3:-14652455	None:intergenic
TCTTCAAAACTCTGGATCAG+AGG	0.579120	4.3:-14652408	MS.gene023552:CDS
GGGAGATCATAATAACATGC+AGG	0.579180	4.3:-14652387	MS.gene023552:CDS
AAATGGCTGAACTTAGCCAC+AGG	0.585823	4.3:-14652154	MS.gene023552:CDS
CCAGATGAATAAGTTCCACC+AGG	0.607477	4.3:+14652440	None:intergenic
ATTGTGGATTCTTGGACACA+TGG	0.610654	4.3:-14652052	MS.gene023552:CDS
ACATAACAGATCAAAAGAAG+AGG	0.628528	4.3:-14652361	MS.gene023552:CDS
ATTCAAATAAGCGCAAACGT+TGG	0.641513	4.3:-14652233	MS.gene023552:CDS
CATTAAGTGATTGCAACCTG+TGG	0.653582	4.3:+14652138	None:intergenic
TACTCAGATGTACCTGAACG+TGG	0.654069	4.3:-14652204	MS.gene023552:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATCTGAAAATGCTATTCTT+AGG	-	chr4.3:14652308-14652327	MS.gene023552:CDS	25.0%
!	ATCTGAAAATGCTATTCTTA+GGG	-	chr4.3:14652309-14652328	MS.gene023552:CDS	25.0%
	ACATAACAGATCAAAAGAAG+AGG	-	chr4.3:14652128-14652147	MS.gene023552:CDS	30.0%
	GATCAATTGTTCAATGATTG+TGG	-	chr4.3:14652421-14652440	MS.gene023552:CDS	30.0%
!!!	ATCAAGTAACTCACTTTTTC+AGG	-	chr4.3:14652393-14652412	MS.gene023552:CDS	30.0%
!!!	CTTTTTTCAGCTGTTCAATT+TGG	+	chr4.3:14652231-14652250	None:intergenic	30.0%
	ATTCAAATAAGCGCAAACGT+TGG	-	chr4.3:14652256-14652275	MS.gene023552:CDS	35.0%
	GATGAAGAAACAGCAACATA+TGG	-	chr4.3:14652192-14652211	MS.gene023552:CDS	35.0%
	GTTCAATGATTGTGGATTCT+TGG	-	chr4.3:14652429-14652448	MS.gene023552:CDS	35.0%
	TGCTATTCTTAGGGTTCAAA+TGG	-	chr4.3:14652318-14652337	MS.gene023552:CDS	35.0%
	ACAAGCTCTCTTCAAAACTC+TGG	-	chr4.3:14652073-14652092	MS.gene023552:CDS	40.0%
	ATTGTGGATTCTTGGACACA+TGG	-	chr4.3:14652437-14652456	MS.gene023552:CDS	40.0%
	CATTAAGTGATTGCAACCTG+TGG	+	chr4.3:14652354-14652373	None:intergenic	40.0%
	CTTCAAAACTCTGGATCAGA+GGG	-	chr4.3:14652082-14652101	MS.gene023552:CDS	40.0%
	GGGAGATCATAATAACATGC+AGG	-	chr4.3:14652102-14652121	MS.gene023552:CDS	40.0%
	TCTTCAAAACTCTGGATCAG+AGG	-	chr4.3:14652081-14652100	MS.gene023552:CDS	40.0%
!	CATTTTCAGATTCCACGTTC+AGG	+	chr4.3:14652300-14652319	None:intergenic	40.0%
!!	TTGATCTTCGTGCTGATTCT+CGG	+	chr4.3:14652171-14652190	None:intergenic	40.0%
	AAATGGCTGAACTTAGCCAC+AGG	-	chr4.3:14652335-14652354	MS.gene023552:CDS	45.0%
	CCAGATGAATAAGTTCCACC+AGG	+	chr4.3:14652052-14652071	None:intergenic	45.0%
	CCTGGTGGAACTTATTCATC+TGG	-	chr4.3:14652049-14652068	MS.gene023552:CDS	45.0%
	GAGAATCAGCACGAAGATCA+AGG	-	chr4.3:14652170-14652189	MS.gene023552:CDS	45.0%
	TACTCAGATGTACCTGAACG+TGG	-	chr4.3:14652285-14652304	MS.gene023552:CDS	45.0%
!!	TCTTCGTGCTGATTCTCGGT+TGG	+	chr4.3:14652167-14652186	None:intergenic	50.0%
	GTTCCACCAGGAGAAGCCAT+AGG	+	chr4.3:14652040-14652059	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	14652040	14652471	14652040	ID=MS.gene023552
chr4.3	mRNA	14652040	14652471	14652040	ID=MS.gene023552.t1;Parent=MS.gene023552
chr4.3	exon	14652040	14652471	14652040	ID=MS.gene023552.t1.exon1;Parent=MS.gene023552.t1
chr4.3	CDS	14652040	14652471	14652040	ID=cds.MS.gene023552.t1;Parent=MS.gene023552.t1

Gene Sequence

>MS.gene023552

ATGGCTTCTCCTGGTGGAACTTATTCATCTGGCACAAGCTCTCTTCAAAACTCTGGATCAGAGGGAGATCATAATAACATGCAGGTGAACATAACAGATCAAAAGAAGAGGAAGAGAATGCAATCCAACCGAGAATCAGCACGAAGATCAAGGATGAAGAAACAGCAACATATGGAAGATCTAAGCAACCAAATTGAACAGCTGAAAAAAGAGAACATTCAAATAAGCGCAAACGTTGGAGTAACTACTCAGATGTACCTGAACGTGGAATCTGAAAATGCTATTCTTAGGGTTCAAATGGCTGAACTTAGCCACAGGTTGCAATCACTTAATGATATTATTCATTACATTGAATCAAGTAACTCACTTTTTCAGGAGACAGATCAATTGTTCAATGATTGTGGATTCTTGGACACATGGAACACTTTTCCT

Protein sequence

>MS.gene023552.t1

MASPGGTYSSGTSSLQNSGSEGDHNNMQVNITDQKKRKRMQSNRESARRSRMKKQQHMEDLSNQIEQLKKENIQISANVGVTTQMYLNVESENAILRVQMAELSHRLQSLNDIIHYIESSNSLFQETDQLFNDCGFLDTWNTFP