AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023746

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023746.t1 MTR_5g067680 93.611 360 19 2 1 359 1 357 0.0 682
MS.gene023746.t1 MTR_5g073620 51.163 129 60 3 149 275 616 743 3.22e-32 128
MS.gene023746.t1 MTR_3g090860 53.763 93 41 2 173 263 100 192 5.15e-24 101
MS.gene023746.t1 MTR_8g099350 59.459 74 30 0 200 273 128 201 1.04e-23 100
MS.gene023746.t1 MTR_7g117200 40.299 134 71 2 197 329 111 236 7.25e-23 97.8
MS.gene023746.t1 MTR_8g027860 59.091 66 27 0 204 269 136 201 3.90e-20 90.5
MS.gene023746.t1 MTR_7g073430 41.667 108 57 2 161 263 75 181 4.76e-18 83.6
MS.gene023746.t1 MTR_7g073380 37.963 108 62 1 161 263 75 182 1.67e-17 82.4
MS.gene023746.t1 MTR_8g005750 52.239 67 32 0 199 265 98 164 3.85e-17 80.9
MS.gene023746.t1 MTR_3g093830 36.975 119 60 3 160 270 82 193 4.96e-17 81.3
MS.gene023746.t1 MTR_2g016770 42.169 83 46 1 179 261 10 90 1.14e-16 78.2
MS.gene023746.t1 MTR_0002s1250 47.692 65 34 0 199 263 106 170 2.85e-16 78.2
MS.gene023746.t1 MTR_2g016770 43.210 81 44 1 181 261 4 82 3.60e-16 76.6
MS.gene023746.t1 MTR_5g043880 42.857 105 53 4 169 272 90 188 3.98e-16 78.2
MS.gene023746.t1 MTR_2g075610 50.000 64 32 0 204 267 29 92 4.75e-16 75.9
MS.gene023746.t1 MTR_7g079010 54.688 64 24 2 204 265 141 201 1.16e-15 75.5
MS.gene023746.t1 MTR_2g075750 49.231 65 33 0 204 268 28 92 1.22e-15 74.7
MS.gene023746.t1 MTR_2g075680 53.968 63 29 0 204 266 148 210 1.23e-15 77.0
MS.gene023746.t1 MTR_7g028415 56.897 58 22 1 204 261 93 147 1.61e-15 73.9
MS.gene023746.t1 MTR_7g028710 56.897 58 22 1 204 261 93 147 1.61e-15 73.9
MS.gene023746.t1 MTR_1g009613 43.182 88 44 3 177 261 233 317 1.68e-15 77.0
MS.gene023746.t1 MTR_8g092140 52.542 59 25 1 204 262 160 215 1.92e-15 75.1
MS.gene023746.t1 MTR_2g032030 39.796 98 53 2 168 261 227 322 2.52e-15 77.0
MS.gene023746.t1 MTR_4g127040 47.059 85 41 3 178 261 219 300 2.98e-15 75.9
MS.gene023746.t1 MTR_2g032030 39.394 99 54 2 167 261 226 322 3.23e-15 77.0
MS.gene023746.t1 MTR_3g009470 52.459 61 25 2 201 261 277 333 3.60e-15 77.0
MS.gene023746.t1 MTR_3g009470 51.515 66 26 3 196 261 274 333 4.49e-15 77.0
MS.gene023746.t1 MTR_4g094908 45.882 85 42 3 177 261 241 321 6.83e-15 75.1
MS.gene023746.t1 MTR_5g067700 45.588 68 37 0 205 272 107 174 8.34e-15 73.6
MS.gene023746.t1 MTR_7g100510 49.275 69 29 3 194 261 179 242 9.06e-15 75.5
MS.gene023746.t1 MTR_2g075700 43.902 82 45 1 186 267 127 207 1.60e-14 73.9
MS.gene023746.t1 MTR_1g070455 45.122 82 40 3 193 272 281 359 1.69e-14 74.3
MS.gene023746.t1 MTR_3g108960 54.237 59 25 1 203 261 313 369 1.74e-14 74.3
MS.gene023746.t1 MTR_3g095080 53.448 58 24 1 204 261 168 222 1.99e-14 72.4
MS.gene023746.t1 MTR_8g087000 47.692 65 32 1 197 261 70 132 2.04e-14 72.4
MS.gene023746.t1 MTR_8g087000 47.692 65 32 1 197 261 69 131 2.39e-14 72.4
MS.gene023746.t1 MTR_5g091390 54.098 61 25 1 204 264 157 214 2.66e-14 73.2
MS.gene023746.t1 MTR_3g095080 53.448 58 24 1 204 261 144 198 2.73e-14 71.6
MS.gene023746.t1 MTR_7g011820 48.387 62 28 2 200 261 224 281 3.14e-14 73.9
MS.gene023746.t1 MTR_7g011820 48.276 58 27 1 204 261 410 464 9.93e-12 66.6
MS.gene023746.t1 MTR_8g096730 50.847 59 26 1 204 262 70 125 3.38e-14 69.7
MS.gene023746.t1 MTR_0117s0040 54.237 59 25 1 203 261 73 129 3.48e-14 72.0
MS.gene023746.t1 MTR_7g062220 52.542 59 25 1 204 262 124 179 3.68e-14 72.8
MS.gene023746.t1 MTR_4g094588 55.172 58 23 1 204 261 178 232 4.86e-14 72.8
MS.gene023746.t1 MTR_5g016610 42.353 85 45 3 178 261 173 254 6.53e-14 71.6
MS.gene023746.t1 MTR_2g083870 40.566 106 51 4 173 273 47 145 6.86e-14 71.2
MS.gene023746.t1 MTR_5g042920 47.059 68 32 2 194 261 141 204 9.27e-14 72.4
MS.gene023746.t1 MTR_5g042920 46.552 58 28 1 204 261 320 374 6.32e-11 63.9
MS.gene023746.t1 MTR_8g007270 53.448 58 24 1 204 261 154 208 1.15e-13 70.1
MS.gene023746.t1 MTR_2g075690 49.206 63 32 0 204 266 141 203 1.52e-13 70.9
MS.gene023746.t1 MTR_7g080020 50.000 58 25 2 204 261 280 333 7.13e-13 70.1
MS.gene023746.t1 MTR_7g109800 40.404 99 49 3 204 295 145 240 7.56e-13 68.6
MS.gene023746.t1 MTR_2g024350 49.153 59 28 1 203 261 7 63 8.61e-13 67.4
MS.gene023746.t1 MTR_5g094430 48.276 58 27 1 204 261 364 418 1.07e-12 69.3
MS.gene023746.t1 MTR_3g031220 46.377 69 31 3 194 261 188 251 1.34e-12 68.6
MS.gene023746.t1 MTR_3g056100 48.276 58 27 1 204 261 402 456 1.36e-12 69.3
MS.gene023746.t1 MTR_5g094430 48.276 58 27 1 204 261 230 284 1.41e-12 68.9
MS.gene023746.t1 MTR_3g056100 48.276 58 27 1 204 261 415 469 1.41e-12 69.3
MS.gene023746.t1 MTR_5g094430 48.276 58 27 1 204 261 252 306 1.51e-12 68.6
MS.gene023746.t1 MTR_3g056100 48.276 58 27 1 204 261 258 312 1.87e-12 68.6
MS.gene023746.t1 MTR_3g056100 48.276 58 27 1 204 261 271 325 1.98e-12 68.6
MS.gene023746.t1 MTR_1g086790 51.724 58 25 1 204 261 158 212 2.09e-12 67.8
MS.gene023746.t1 MTR_3g031220 36.697 109 53 4 194 301 188 281 2.15e-12 68.6
MS.gene023746.t1 MTR_3g031220 46.552 58 28 1 204 261 365 419 6.73e-11 63.9
MS.gene023746.t1 MTR_8g069935 42.391 92 48 3 170 261 300 386 2.60e-12 68.2
MS.gene023746.t1 MTR_5g074400 48.276 58 26 2 204 261 243 296 2.72e-12 68.2
MS.gene023746.t1 MTR_5g074400 48.276 58 27 1 204 261 420 474 8.76e-12 66.6
MS.gene023746.t1 MTR_4g082580 35.106 94 50 2 198 291 139 221 3.87e-12 67.4
MS.gene023746.t1 MTR_4g082580 48.276 58 27 1 204 261 278 332 1.85e-11 65.1
MS.gene023746.t1 MTR_2g088000 53.448 58 25 1 205 262 350 405 3.91e-12 67.8
MS.gene023746.t1 MTR_4g132430 46.575 73 27 2 205 277 128 188 6.58e-12 66.6
MS.gene023746.t1 MTR_7g071120 44.928 69 34 2 193 261 155 219 6.85e-12 67.0
MS.gene023746.t1 MTR_4g132430 46.575 73 27 2 205 277 262 322 7.32e-12 66.6
MS.gene023746.t1 MTR_4g013260 44.828 58 29 1 204 261 184 238 7.53e-12 66.6
MS.gene023746.t1 MTR_3g053330 50.877 57 26 1 205 261 252 306 7.61e-12 66.6
MS.gene023746.t1 MTR_2g033820 44.737 76 33 2 204 279 203 269 7.78e-12 66.2
MS.gene023746.t1 MTR_1g013760 49.123 57 27 1 205 261 141 195 8.95e-12 65.5
MS.gene023746.t1 MTR_2g023930 52.632 57 25 1 205 261 306 360 9.72e-12 66.6
MS.gene023746.t1 MTR_7g110720 52.632 57 25 1 205 261 217 271 1.15e-11 65.9
MS.gene023746.t1 MTR_7g110720 52.632 57 25 1 205 261 190 244 1.28e-11 65.9
MS.gene023746.t1 MTR_2g024380 47.458 59 29 1 203 261 250 306 1.30e-11 65.9
MS.gene023746.t1 MTR_8g098945 52.632 57 25 1 205 261 130 184 1.39e-11 64.7
MS.gene023746.t1 MTR_3g106060 44.615 65 34 1 197 261 131 193 1.92e-11 64.7
MS.gene023746.t1 MTR_8g067650 50.000 58 27 1 204 261 339 394 2.04e-11 65.5
MS.gene023746.t1 MTR_8g067635 50.000 58 27 1 204 261 349 404 2.21e-11 65.5
MS.gene023746.t1 MTR_8g028565 52.632 57 25 1 205 261 156 210 2.33e-11 64.7
MS.gene023746.t1 MTR_4g130900 47.368 57 27 1 205 261 360 413 2.98e-11 65.1
MS.gene023746.t1 MTR_1g099600 49.123 57 27 1 205 261 186 240 4.08e-11 63.5
MS.gene023746.t1 MTR_5g097490 47.368 57 27 1 205 261 253 306 5.11e-11 63.9
MS.gene023746.t1 MTR_8g032510 43.333 60 34 0 204 263 119 178 5.33e-11 63.2
MS.gene023746.t1 MTR_7g079040 41.379 87 42 3 175 261 132 209 5.92e-11 63.2
MS.gene023746.t1 MTR_4g107970 52.632 57 25 1 205 261 179 233 8.43e-11 63.5
MS.gene023746.t1 MTR_7g109600 47.458 59 29 1 203 261 52 108 8.89e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023746.t1 AT2G40740 34.188 351 150 11 1 345 11 286 2.59e-51 172
MS.gene023746.t1 AT2G40740 34.188 351 150 11 1 345 20 295 3.71e-51 172
MS.gene023746.t1 AT4G11070 52.174 92 42 2 180 270 85 175 2.55e-22 95.5
MS.gene023746.t1 AT4G11070 52.174 92 42 2 180 270 117 207 4.01e-22 95.5
MS.gene023746.t1 AT4G23810 59.420 69 28 0 200 268 154 222 6.25e-22 95.5
MS.gene023746.t1 AT5G24110 45.918 98 52 1 177 274 87 183 2.05e-21 93.6
MS.gene023746.t1 AT2G40740 42.188 128 42 5 220 345 145 242 4.29e-21 91.7
MS.gene023746.t1 AT2G46400 36.429 140 80 4 148 287 56 186 9.64e-20 88.6
MS.gene023746.t1 AT3G56400 52.174 69 33 0 204 272 120 188 3.20e-19 87.0
MS.gene023746.t1 AT1G66550 53.968 63 29 0 201 263 100 162 2.59e-18 84.0
MS.gene023746.t1 AT1G66550 53.968 63 29 0 201 263 105 167 3.49e-18 83.6
MS.gene023746.t1 AT1G66560 43.956 91 44 1 173 263 79 162 7.19e-18 82.4
MS.gene023746.t1 AT1G64000 56.897 58 22 1 204 261 114 168 6.85e-17 78.6
MS.gene023746.t1 AT2G40750 53.333 60 28 0 204 263 152 211 2.55e-16 79.7
MS.gene023746.t1 AT5G41570 56.897 58 22 1 204 261 98 152 2.86e-16 76.3
MS.gene023746.t1 AT4G31550 44.048 84 44 2 178 261 220 300 4.18e-16 78.6
MS.gene023746.t1 AT5G01900 54.237 59 27 0 205 263 111 169 4.24e-16 77.8
MS.gene023746.t1 AT4G31550 48.235 85 40 3 177 261 221 301 5.15e-16 78.6
MS.gene023746.t1 AT1G29280 44.086 93 41 4 175 261 42 129 1.05e-15 76.6
MS.gene023746.t1 AT2G24570 46.667 90 44 3 177 266 218 303 1.10e-15 77.4
MS.gene023746.t1 AT2G46130 55.172 58 23 1 204 261 30 84 1.41e-15 72.4
MS.gene023746.t1 AT2G30590 32.639 144 89 5 122 261 229 368 1.90e-15 77.4
MS.gene023746.t1 AT2G23320 41.176 85 46 3 178 261 214 295 1.96e-15 76.6
MS.gene023746.t1 AT1G69310 49.254 67 30 2 204 270 147 209 2.86e-15 75.9
MS.gene023746.t1 AT1G69310 49.254 67 30 2 204 270 147 209 2.86e-15 75.9
MS.gene023746.t1 AT1G69310 49.254 67 30 2 204 270 147 209 2.86e-15 75.9
MS.gene023746.t1 AT4G26440 48.052 77 27 3 202 278 176 239 4.09e-15 77.0
MS.gene023746.t1 AT4G18170 27.925 265 158 9 75 337 59 292 8.17e-15 74.7
MS.gene023746.t1 AT5G28650 45.882 85 42 3 178 261 236 317 1.05e-14 74.7
MS.gene023746.t1 AT4G39410 42.857 84 38 2 204 280 223 303 1.18e-14 74.3
MS.gene023746.t1 AT2G34830 43.023 86 43 2 180 261 187 270 1.56e-14 74.7
MS.gene023746.t1 AT2G34830 43.023 86 43 2 180 261 196 279 1.66e-14 74.7
MS.gene023746.t1 AT5G22570 49.153 59 30 0 205 263 111 169 1.91e-14 73.6
MS.gene023746.t1 AT5G45260 40.476 84 46 1 182 261 1182 1265 2.21e-14 75.1
MS.gene023746.t1 AT3G04670 41.237 97 53 3 178 273 236 329 3.30e-14 73.2
MS.gene023746.t1 AT2G37260 43.925 107 53 3 172 278 321 420 3.45e-14 73.9
MS.gene023746.t1 AT2G37260 54.386 57 22 2 205 261 166 218 1.94e-13 71.6
MS.gene023746.t1 AT2G37260 43.925 107 53 3 172 278 241 340 3.78e-14 73.2
MS.gene023746.t1 AT2G37260 54.386 57 22 2 205 261 86 138 2.10e-13 70.9
MS.gene023746.t1 AT1G66600 40.659 91 46 2 173 263 80 162 3.88e-14 71.6
MS.gene023746.t1 AT3G58710 43.478 92 43 4 175 261 39 126 3.95e-14 72.4
MS.gene023746.t1 AT2G37260 43.925 107 53 3 172 278 239 338 3.98e-14 73.2
MS.gene023746.t1 AT2G37260 54.386 57 22 2 205 261 84 136 2.12e-13 70.9
MS.gene023746.t1 AT2G44745 53.448 58 24 1 204 261 145 199 4.20e-14 71.2
MS.gene023746.t1 AT1G29860 55.172 58 23 1 204 261 136 190 5.36e-14 72.0
MS.gene023746.t1 AT1G80590 50.847 59 29 0 205 263 86 144 6.58e-14 70.9
MS.gene023746.t1 AT1G30650 43.023 86 43 2 180 261 189 272 7.25e-14 72.8
MS.gene023746.t1 AT2G03340 48.529 68 29 3 195 261 241 303 7.90e-14 72.8
MS.gene023746.t1 AT2G03340 40.964 83 46 1 204 286 415 494 2.39e-11 65.5
MS.gene023746.t1 AT3G01080 48.387 62 28 2 200 261 201 258 8.55e-14 72.0
MS.gene023746.t1 AT3G58710 40.426 94 48 3 173 261 35 125 1.07e-13 70.9
MS.gene023746.t1 AT3G01080 48.387 62 28 2 200 261 201 258 2.86e-13 71.2
MS.gene023746.t1 AT3G01080 46.552 58 28 1 204 261 344 398 9.96e-11 63.2
MS.gene023746.t1 AT5G46350 51.724 58 25 1 204 261 183 237 2.98e-13 70.5
MS.gene023746.t1 AT5G13080 53.448 58 24 1 204 261 67 121 3.50e-13 66.6
MS.gene023746.t1 AT3G01080 48.387 62 28 2 200 261 163 220 4.20e-13 70.5
MS.gene023746.t1 AT2G38470 43.182 88 40 4 180 261 154 237 4.26e-13 70.9
MS.gene023746.t1 AT1G13960 45.833 72 33 3 191 261 216 282 4.31e-13 70.9
MS.gene023746.t1 AT1G13960 46.552 58 28 1 204 261 409 463 8.81e-11 63.5
MS.gene023746.t1 AT1G13960 45.833 72 33 3 191 261 63 129 7.96e-13 69.3
MS.gene023746.t1 AT5G52830 48.780 82 34 3 180 261 147 220 9.96e-13 68.9
MS.gene023746.t1 AT4G24240 41.758 91 49 3 172 261 249 336 1.01e-12 68.9
MS.gene023746.t1 AT5G26170 46.552 58 28 1 204 261 113 167 1.22e-12 65.9
MS.gene023746.t1 AT4G31800 43.284 67 36 1 195 261 102 166 1.77e-12 67.0
MS.gene023746.t1 AT4G04450 42.045 88 38 3 205 292 199 273 1.89e-12 68.6
MS.gene023746.t1 AT5G56270 45.455 77 29 3 202 278 271 334 1.92e-12 68.9
MS.gene023746.t1 AT5G49520 50.000 58 26 1 204 261 221 275 2.00e-12 68.2
MS.gene023746.t1 AT4G26640 48.276 58 27 1 204 261 341 395 2.12e-12 68.6
MS.gene023746.t1 AT4G26640 48.276 58 27 1 204 261 381 435 2.16e-12 68.6
MS.gene023746.t1 AT4G23550 52.632 57 25 1 205 261 141 195 2.20e-12 67.4
MS.gene023746.t1 AT4G26640 48.276 58 27 1 204 261 205 259 2.22e-12 68.2
MS.gene023746.t1 AT4G26640 48.276 58 27 1 204 261 205 259 2.22e-12 68.2
MS.gene023746.t1 AT4G26640 48.276 58 27 1 204 261 205 259 2.22e-12 68.2
MS.gene023746.t1 AT4G26640 48.276 58 27 1 204 261 205 259 2.22e-12 68.2
MS.gene023746.t1 AT4G26640 48.276 58 27 1 204 261 309 363 2.41e-12 68.2
MS.gene023746.t1 AT4G31800 43.284 67 36 1 195 261 167 231 2.46e-12 67.4
MS.gene023746.t1 AT4G04450 42.045 88 38 3 205 292 293 367 2.70e-12 68.2
MS.gene023746.t1 AT4G30935 42.857 77 40 2 205 281 332 404 3.34e-12 67.8
MS.gene023746.t1 AT2G47260 38.462 91 53 1 179 269 149 236 7.49e-12 66.2
MS.gene023746.t1 AT4G01250 38.554 83 44 2 179 261 108 183 1.13e-11 65.5
MS.gene023746.t1 AT1G62300 52.632 57 25 1 205 261 313 367 1.73e-11 65.9
MS.gene023746.t1 AT4G01720 52.632 57 25 1 205 261 240 294 2.29e-11 65.5
MS.gene023746.t1 AT2G30250 52.632 57 24 1 205 261 329 382 3.13e-11 64.7
MS.gene023746.t1 AT5G43290 49.153 59 27 1 204 262 114 169 3.67e-11 63.5
MS.gene023746.t1 AT5G45050 42.353 85 42 2 177 261 1158 1235 4.24e-11 65.1
MS.gene023746.t1 AT4G22070 52.632 57 25 1 205 261 298 352 4.36e-11 64.7
MS.gene023746.t1 AT4G22070 52.632 57 25 1 205 261 298 352 4.36e-11 64.7
MS.gene023746.t1 AT4G22070 52.632 57 25 1 205 261 298 352 4.36e-11 64.7
MS.gene023746.t1 AT4G22070 52.632 57 25 1 205 261 298 352 4.36e-11 64.7
MS.gene023746.t1 AT1G55600 44.776 67 34 1 195 261 298 361 4.46e-11 64.3
MS.gene023746.t1 AT5G45050 42.353 85 42 2 177 261 1130 1207 4.66e-11 65.1
MS.gene023746.t1 AT2G04880 47.368 57 27 1 205 261 284 337 4.74e-11 64.3
MS.gene023746.t1 AT2G04880 51.724 58 24 2 204 261 111 164 9.22e-11 63.5
MS.gene023746.t1 AT5G45050 42.353 85 42 2 177 261 1019 1096 4.75e-11 64.7
MS.gene023746.t1 AT2G04880 47.368 57 27 1 205 261 308 361 4.94e-11 64.3
MS.gene023746.t1 AT2G04880 51.724 58 24 2 204 261 111 164 8.62e-11 63.5
MS.gene023746.t1 AT2G25000 37.500 80 48 1 182 261 81 158 5.28e-11 62.4
MS.gene023746.t1 AT2G25000 37.500 80 48 1 182 261 81 158 5.28e-11 62.4
MS.gene023746.t1 AT2G25000 37.500 80 48 1 182 261 103 180 9.10e-11 62.0

Find 95 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGAGAATAGCATTTC+AGG 0.221599 5.4:-52819764 None:intergenic
AAGTCTTTGCACTTGCTTCT+TGG 0.236235 5.4:-52822564 None:intergenic
TATGTTACTTCAACTATGTT+AGG 0.240903 5.4:-52822596 None:intergenic
CATCATCATTACTCTTTCTT+TGG 0.249534 5.4:-52819816 None:intergenic
GATAGATCAAAAGGGTTAGA+AGG 0.266264 5.4:+52820142 MS.gene023746:CDS
GGCTTTACTTGGAGAAAATA+TGG 0.298786 5.4:+52821857 MS.gene023746:CDS
TGATTATCCGGTGGCCGATA+TGG 0.327197 5.4:+52822805 MS.gene023746:CDS
AACTTTGAGGATTGGTGAAA+TGG 0.332908 5.4:+52820096 MS.gene023746:CDS
AAAACTCAACATGCTCAAAT+TGG 0.341836 5.4:+52819923 MS.gene023746:CDS
TCACCGCAGGTGGCCACCCT+TGG 0.347403 5.4:-52822724 None:intergenic
CTTTGCACTTGCTTCTTGGC+TGG 0.354716 5.4:-52822560 None:intergenic
TTCAGGGTGGTTGGCCATGT+TGG 0.368022 5.4:-52819747 None:intergenic
CCTTTATCAGAAGTTACTAT+AGG 0.370143 5.4:+52822510 MS.gene023746:intron
TAGTAGCAGTGGCAATAGTA+TGG 0.375087 5.4:+52822850 MS.gene023746:CDS
CTCCAAGTAAAGCCATCTTC+TGG 0.382520 5.4:-52821848 None:intergenic
ATTAGAACACTCACATCAAA+AGG 0.383819 5.4:-52820010 None:intergenic
ACGTTCAGTTGTTAAGGTCT+AGG 0.384626 5.4:+52820071 MS.gene023746:CDS
ATTTGAGCAAAGGAATTAGA+AGG 0.385061 5.4:-52819875 None:intergenic
GAAGGTTTAGATAGATCAAA+AGG 0.386309 5.4:+52820133 MS.gene023746:CDS
TTCAATGCATGCACATTCAA+TGG 0.387023 5.4:+52819964 MS.gene023746:CDS
TAGCAGCAGGAAGAATGATT+TGG 0.389860 5.4:+52821772 MS.gene023746:intron
CACCAGAAGATGGCTTTACT+TGG 0.405190 5.4:+52821846 MS.gene023746:CDS
TCAGGGTGGTTGGCCATGTT+GGG 0.411278 5.4:-52819746 None:intergenic
AAGATTCGGCATTGATTATC+CGG 0.412157 5.4:+52822793 MS.gene023746:CDS
CTGCGGTGAACCACGACCTC+AGG 0.426028 5.4:+52822738 MS.gene023746:CDS
GTGTTTCCAATCTGAGGTGC+TGG 0.434455 5.4:-52821815 None:intergenic
GGAAGAAATGGCAGTTTCAA+TGG 0.437250 5.4:-52822702 None:intergenic
ATGTTACTTCAACTATGTTA+GGG 0.440557 5.4:-52822595 None:intergenic
CATGCTCAAATTGGTGGAAG+TGG 0.441412 5.4:+52819932 MS.gene023746:CDS
CACCGCAGGTGGCCACCCTT+GGG 0.445492 5.4:-52822723 None:intergenic
CTAATGTTCACAAGTAGGTT+TGG 0.447789 5.4:-52822677 None:intergenic
GAACATTACATCGGCCATAT+CGG 0.452060 5.4:-52822819 None:intergenic
ACAAGTAGGTTTGGTGGTGG+TGG 0.452659 5.4:-52822668 None:intergenic
ACTTTGAGGATTGGTGAAAT+GGG 0.454574 5.4:+52820097 MS.gene023746:CDS
ATATGATGATGGTGCTGTTA+AGG 0.457432 5.4:-52822645 None:intergenic
TGGCCACCCTTGGGAAGAAA+TGG 0.460403 5.4:-52822714 None:intergenic
GAAATTCATGACTTCTTCCA+TGG 0.461625 5.4:-52819678 None:intergenic
TGAAATGGGTGGGAGGGATG+TGG 0.472056 5.4:+52820111 MS.gene023746:CDS
GGTGGTGGTGGATATGATGA+TGG 0.483740 5.4:-52822656 None:intergenic
TAAGGTCTAGGGAAACTTTG+AGG 0.489231 5.4:+52820083 MS.gene023746:CDS
CTGAGGTCGTGGTTCACCGC+AGG 0.489237 5.4:-52822737 None:intergenic
CGTTCAGTTGTTAAGGTCTA+GGG 0.489911 5.4:+52820072 MS.gene023746:CDS
GGACCCTCTACTTCAAGATT+CGG 0.492644 5.4:+52822779 MS.gene023746:CDS
CGTCTCCGCCCCTGAGGTCG+TGG 0.495381 5.4:-52822748 None:intergenic
CTCAGGGGCGGAGACGGTAC+TGG 0.503754 5.4:+52822755 MS.gene023746:CDS
TCTAGGGAAACTTTGAGGAT+TGG 0.504126 5.4:+52820088 MS.gene023746:CDS
ATGCATCCAGCACCTCAGAT+TGG 0.509798 5.4:+52821809 MS.gene023746:CDS
GCGGTGACGTTCAGTTGTTA+AGG 0.512945 5.4:+52820065 MS.gene023746:CDS
CACGACCTCAGGGGCGGAGA+CGG 0.513045 5.4:+52822749 MS.gene023746:CDS
TTCCCAAGGGTGGCCACCTG+CGG 0.515927 5.4:+52822721 MS.gene023746:CDS
TGGATGAGAATAGCATTTCA+GGG 0.524108 5.4:-52819763 None:intergenic
ATAGTTGAAGTAACATATAG+AGG 0.529154 5.4:+52822602 MS.gene023746:CDS
ATCTCAGTGTTTCCAATCTG+AGG 0.531039 5.4:-52821821 None:intergenic
ACTCAACATGCTCAAATTGG+TGG 0.531204 5.4:+52819926 MS.gene023746:CDS
ACTGAGATGCCACCAGAAGA+TGG 0.534882 5.4:+52821836 MS.gene023746:CDS
GAATAGCATTTCAGGGTGGT+TGG 0.535922 5.4:-52819756 None:intergenic
ATGATAAGTGGCGGTGGTGA+TGG 0.538282 5.4:+52820043 MS.gene023746:CDS
ATTGAGAATAAGATGATAAG+TGG 0.540967 5.4:+52820031 MS.gene023746:CDS
GAAACTGCCATTTCTTCCCA+AGG 0.545394 5.4:+52822707 MS.gene023746:CDS
TGGTACTAATGTTCACAAGT+AGG 0.547624 5.4:-52822682 None:intergenic
AAGGTTTAGATAGATCAAAA+GGG 0.550612 5.4:+52820134 MS.gene023746:CDS
TGCGGTGAACCACGACCTCA+GGG 0.552440 5.4:+52822739 MS.gene023746:CDS
AGGTCGTGGTTCACCGCAGG+TGG 0.554696 5.4:-52822734 None:intergenic
TACTTGGCCGCAAGCACCCA+AGG 0.555851 5.4:+52821891 MS.gene023746:CDS
ATGTTCACAAGTAGGTTTGG+TGG 0.557977 5.4:-52822674 None:intergenic
TAATTTACCTTGGGTGCTTG+CGG 0.559902 5.4:-52821898 None:intergenic
TTGAGGATTGGTGAAATGGG+TGG 0.562645 5.4:+52820100 MS.gene023746:CDS
AGGGGCGGAGACGGTACTGG+TGG 0.562847 5.4:+52822758 MS.gene023746:CDS
TGAGGATTGGTGAAATGGGT+GGG 0.564470 5.4:+52820101 MS.gene023746:CDS
GGCCGATGTAATGTTCAACT+CGG 0.582893 5.4:+52822826 MS.gene023746:CDS
AAACTGCCATTTCTTCCCAA+GGG 0.586934 5.4:+52822708 MS.gene023746:CDS
GGATTGGTGAAATGGGTGGG+AGG 0.593788 5.4:+52820104 MS.gene023746:CDS
ATGCCGAATCTTGAAGTAGA+GGG 0.595101 5.4:-52822782 None:intergenic
TTAGAACACTCACATCAAAA+GGG 0.595617 5.4:-52820009 None:intergenic
ATGGGTGGGAGGGATGTGGA+AGG 0.603019 5.4:+52820115 MS.gene023746:CDS
GATTGGTGAAATGGGTGGGA+GGG 0.603257 5.4:+52820105 MS.gene023746:CDS
CTGCCATTTCTTCCCAAGGG+TGG 0.604947 5.4:+52822711 MS.gene023746:CDS
TTCAACTCGGGTAGTAGCAG+TGG 0.607187 5.4:+52822839 MS.gene023746:CDS
ATGAGACAGAATTTGAGCAA+AGG 0.616039 5.4:-52819885 None:intergenic
AGATAAAGGTGAACCTGATG+AGG 0.619424 5.4:+52822895 MS.gene023746:CDS
TTCACAAGTAGGTTTGGTGG+TGG 0.620288 5.4:-52822671 None:intergenic
GCCGATGTAATGTTCAACTC+GGG 0.623899 5.4:+52822827 MS.gene023746:CDS
AATGCCGAATCTTGAAGTAG+AGG 0.627530 5.4:-52822783 None:intergenic
CAAGTAAAGCCATCTTCTGG+TGG 0.629125 5.4:-52821845 None:intergenic
GAGAATAAGATGATAAGTGG+CGG 0.650628 5.4:+52820034 MS.gene023746:CDS
AATAAGATGATAAGTGGCGG+TGG 0.651861 5.4:+52820037 MS.gene023746:CDS
ACATCGGCCATATCGGCCAC+CGG 0.656162 5.4:-52822812 None:intergenic
GTGAACCACGACCTCAGGGG+CGG 0.657610 5.4:+52822743 MS.gene023746:CDS
ATAAGTGGCGGTGGTGATGG+CGG 0.661391 5.4:+52820046 MS.gene023746:CDS
TGAACTAGAACTACCCAACA+TGG 0.663075 5.4:+52819733 MS.gene023746:CDS
ATTCGGCATTGATTATCCGG+TGG 0.666772 5.4:+52822796 MS.gene023746:CDS
CAGTACCGTCTCCGCCCCTG+AGG 0.681574 5.4:-52822754 None:intergenic
ACCCGAGTTGAACATTACAT+CGG 0.690614 5.4:-52822828 None:intergenic
GCGGTGAACCACGACCTCAG+GGG 0.741552 5.4:+52822740 MS.gene023746:CDS
ATGAGAATAGCATTTCAGGG+TGG 0.763743 5.4:-52819760 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTGATTTGAATTTTTT+TGG + chr5.4:52821303-52821322 MS.gene023746:intron 10.0%
!!! GTTTTTATTATATAATTTGT+TGG + chr5.4:52822467-52822486 MS.gene023746:intron 10.0%
!!! TCAAATCAATTTTTTTTTTA+TGG - chr5.4:52821296-52821315 None:intergenic 10.0%
!!! TTTTACATAAAATTATAAGT+AGG + chr5.4:52821673-52821692 MS.gene023746:intron 10.0%
!! AAATATTTAACAAGTAATCA+AGG - chr5.4:52822257-52822276 None:intergenic 15.0%
!! AATTATTGTTAATTTACCTT+GGG - chr5.4:52821910-52821929 None:intergenic 15.0%
!! AGAATTGTACTTTATAATAT+AGG + chr5.4:52821934-52821953 MS.gene023746:intron 15.0%
!! GAATTGTACTTTATAATATA+GGG + chr5.4:52821935-52821954 MS.gene023746:intron 15.0%
!! TAATTATTGTTAATTTACCT+TGG - chr5.4:52821911-52821930 None:intergenic 15.0%
!! TCAAAGTTTCATATATTAAA+TGG + chr5.4:52820287-52820306 MS.gene023746:intron 15.0%
!!! AGTAAAGTGAATTTTTTTTT+TGG - chr5.4:52821586-52821605 None:intergenic 15.0%
!!! TCACTAGTTAATTATTTTAA+TGG + chr5.4:52821482-52821501 MS.gene023746:intron 15.0%
!!! TGTTTATTTTTTAAACATCT+TGG + chr5.4:52822445-52822464 MS.gene023746:intron 15.0%
!! AAAAATCTTATGGAAATGAT+TGG - chr5.4:52820797-52820816 None:intergenic 20.0%
!! AAATTTATATATGCAAGATC+CGG + chr5.4:52821533-52821552 MS.gene023746:intron 20.0%
!! AAGAAATATTCTTAGAAGAA+AGG - chr5.4:52822043-52822062 None:intergenic 20.0%
!! AATAGTTTGACACAATTATT+AGG + chr5.4:52821610-52821629 MS.gene023746:intron 20.0%
!! ACTATTTACATTCATGTATA+AGG + chr5.4:52822143-52822162 MS.gene023746:intron 20.0%
!! CAATTATTAGGAAGTTAATA+TGG + chr5.4:52821622-52821641 MS.gene023746:intron 20.0%
!! CTATTTACATTCATGTATAA+GGG + chr5.4:52822144-52822163 MS.gene023746:intron 20.0%
!! TTTCTTTCAATTATTGTTGT+TGG - chr5.4:52821017-52821036 None:intergenic 20.0%
!!! ATTTCTAAGGTATTGAATTT+AGG + chr5.4:52820396-52820415 MS.gene023746:intron 20.0%
!!! TTATACACATACCATTTTTT+TGG - chr5.4:52820205-52820224 None:intergenic 20.0%
!!! TTTCTTCTAAGAATATTTCT+TGG + chr5.4:52822042-52822061 MS.gene023746:intron 20.0%
!!! TTTTATCTAACTGAAGATAA+AGG + chr5.4:52822881-52822900 MS.gene023746:CDS 20.0%
! AAGGTTTAGATAGATCAAAA+GGG + chr5.4:52820134-52820153 MS.gene023746:CDS 25.0%
! AATTTATATATGCAAGATCC+GGG + chr5.4:52821534-52821553 MS.gene023746:intron 25.0%
! ATAATGTGAAAATCCTAAGT+AGG - chr5.4:52820753-52820772 None:intergenic 25.0%
! ATACATCAAAGTTGAAAGTT+AGG - chr5.4:52820638-52820657 None:intergenic 25.0%
! ATAGTTGAAGTAACATATAG+AGG + chr5.4:52822602-52822621 MS.gene023746:CDS 25.0%
! ATGTTATTGAATCAAAGTCT+TGG + chr5.4:52820333-52820352 MS.gene023746:intron 25.0%
! ATTCAATAACATAGATTGTG+AGG - chr5.4:52820325-52820344 None:intergenic 25.0%
! ATTGAGAATAAGATGATAAG+TGG + chr5.4:52820031-52820050 MS.gene023746:CDS 25.0%
! GTATAATTCAGTTGTTAGAT+TGG + chr5.4:52821644-52821663 MS.gene023746:intron 25.0%
! TAAAAGTAGAGATTGAAATG+AGG + chr5.4:52820859-52820878 MS.gene023746:intron 25.0%
! TAATGTGAAAATCCTAAGTA+GGG - chr5.4:52820752-52820771 None:intergenic 25.0%
! TGAAACTTTCAAACTCTTTA+TGG - chr5.4:52821970-52821989 None:intergenic 25.0%
! TTGCAATTCAACTGAAATTA+AGG - chr5.4:52820566-52820585 None:intergenic 25.0%
!! ATGTAAGATAGAATTTTCCA+TGG + chr5.4:52821137-52821156 MS.gene023746:intron 25.0%
!! ATGTTACTTCAACTATGTTA+GGG - chr5.4:52822598-52822617 None:intergenic 25.0%
!! ATTCTTAGAAGAAAGGTATA+AGG - chr5.4:52822036-52822055 None:intergenic 25.0%
!! CTGGACACAAATATAATTTT+TGG - chr5.4:52822544-52822563 None:intergenic 25.0%
!! TAGGGTGAAAATTTTCATTA+TGG - chr5.4:52820734-52820753 None:intergenic 25.0%
!! TATGTTACTTCAACTATGTT+AGG - chr5.4:52822599-52822618 None:intergenic 25.0%
!! TCCATAAGATTTTTACTAGA+GGG + chr5.4:52820803-52820822 MS.gene023746:intron 25.0%
!! TTCCATAAGATTTTTACTAG+AGG + chr5.4:52820802-52820821 MS.gene023746:intron 25.0%
!!! ATTTTTTTTTTGGTGATGAC+CGG - chr5.4:52821576-52821595 None:intergenic 25.0%
!!! CAACACAAAGATTATTTCTA+AGG + chr5.4:52820383-52820402 MS.gene023746:intron 25.0%
!!! CATATTTGGGTTTTAGTTTA+TGG + chr5.4:52820476-52820495 MS.gene023746:intron 25.0%
!!! TTTTTTGGTGTTTGCTATTT+TGG + chr5.4:52821318-52821337 MS.gene023746:intron 25.0%
AAAACTCAACATGCTCAAAT+TGG + chr5.4:52819923-52819942 MS.gene023746:CDS 30.0%
AACACACCAATAATAACGTA+CGG - chr5.4:52821090-52821109 None:intergenic 30.0%
ACCCAAATATGTAAATTACC+CGG - chr5.4:52820467-52820486 None:intergenic 30.0%
ACCGGGTAATTTACATATTT+GGG + chr5.4:52820463-52820482 MS.gene023746:intron 30.0%
AGATTGAAATGAGGTTAACT+TGG + chr5.4:52820868-52820887 MS.gene023746:intron 30.0%
ATTAGAACACTCACATCAAA+AGG - chr5.4:52820013-52820032 None:intergenic 30.0%
ATTTATATATGCAAGATCCG+GGG + chr5.4:52821535-52821554 MS.gene023746:intron 30.0%
ATTTGAGCAAAGGAATTAGA+AGG - chr5.4:52819878-52819897 None:intergenic 30.0%
CATCATCATTACTCTTTCTT+TGG - chr5.4:52819819-52819838 None:intergenic 30.0%
CATGGTCTTTCTTAGTAATT+AGG + chr5.4:52821155-52821174 MS.gene023746:intron 30.0%
CCTATAGTAACTTCTGATAA+AGG - chr5.4:52822513-52822532 None:intergenic 30.0%
CCTTTATCAGAAGTTACTAT+AGG + chr5.4:52822510-52822529 MS.gene023746:intron 30.0%
CTAATTACTAAGAAAGACCA+TGG - chr5.4:52821157-52821176 None:intergenic 30.0%
GAAGGTTTAGATAGATCAAA+AGG + chr5.4:52820133-52820152 MS.gene023746:CDS 30.0%
GATTACTCAATGTTTGTACT+AGG - chr5.4:52820839-52820858 None:intergenic 30.0%
TAGTTTACATGACCAAAACA+AGG + chr5.4:52821228-52821247 MS.gene023746:intron 30.0%
TATGGCCAAAAAGAAATACT+TGG + chr5.4:52821875-52821894 MS.gene023746:CDS 30.0%
TCCCTCTAGTAAAAATCTTA+TGG - chr5.4:52820807-52820826 None:intergenic 30.0%
TGTGAATGAGTCAATTCATA+GGG - chr5.4:52822103-52822122 None:intergenic 30.0%
TTAGAACACTCACATCAAAA+GGG - chr5.4:52820012-52820031 None:intergenic 30.0%
! ACAAAAGTGAAATGCTTTTG+AGG + chr5.4:52821041-52821060 MS.gene023746:intron 30.0%
! AGGTCTTTGCTATAGTATTT+AGG + chr5.4:52821248-52821267 MS.gene023746:intron 30.0%
! CTTTCAATTATTGTTGTTGG+CGG - chr5.4:52821014-52821033 None:intergenic 30.0%
! GGTATTGAAAGCTTTAGTTT+AGG + chr5.4:52821062-52821081 MS.gene023746:intron 30.0%
! TCTGTAAGATTTTCACTAGA+GGG + chr5.4:52820705-52820724 MS.gene023746:intron 30.0%
! TGAAAATTTTCACCCTACTT+AGG + chr5.4:52820737-52820756 MS.gene023746:intron 30.0%
! TTCTGTAAGATTTTCACTAG+AGG + chr5.4:52820704-52820723 MS.gene023746:intron 30.0%
!! AAGAAAGGTATAAGGTTTTC+TGG - chr5.4:52822028-52822047 None:intergenic 30.0%
!!! GGGTTTTAGTTTATGGTTAT+AGG + chr5.4:52820483-52820502 MS.gene023746:intron 30.0%
!!! TACCATTTTTTTGGTCTTGA+AGG - chr5.4:52820196-52820215 None:intergenic 30.0%
!!! TGCTTTTTCTACATCATCAA+TGG - chr5.4:52819786-52819805 None:intergenic 30.0%
!!! TTTTTTTTTTGGTGATGACC+GGG - chr5.4:52821575-52821594 None:intergenic 30.0%
AATTAGGCAACAAGTCATCA+TGG + chr5.4:52821171-52821190 MS.gene023746:intron 35.0%
ATATGTAAATTACCCGGTGT+TGG - chr5.4:52820461-52820480 None:intergenic 35.0%
ATGAGACAGAATTTGAGCAA+AGG - chr5.4:52819888-52819907 None:intergenic 35.0%
ATGGATGAGAATAGCATTTC+AGG - chr5.4:52819767-52819786 None:intergenic 35.0%
ATGTGTGTGTGATTGAATCT+AGG + chr5.4:52820898-52820917 MS.gene023746:intron 35.0%
CACCGGGTAATTTACATATT+TGG + chr5.4:52820462-52820481 MS.gene023746:intron 35.0%
CACCTTCAAGACCAAAAAAA+TGG + chr5.4:52820191-52820210 MS.gene023746:CDS 35.0%
CTAATGTTCACAAGTAGGTT+TGG - chr5.4:52822680-52822699 None:intergenic 35.0%
GAGAATAAGATGATAAGTGG+CGG + chr5.4:52820034-52820053 MS.gene023746:CDS 35.0%
GATAGATCAAAAGGGTTAGA+AGG + chr5.4:52820142-52820161 MS.gene023746:CDS 35.0%
GGCTTTACTTGGAGAAAATA+TGG + chr5.4:52821857-52821876 MS.gene023746:CDS 35.0%
GTGTCTAAGAACTGTGTATT+TGG + chr5.4:52821344-52821363 MS.gene023746:intron 35.0%
GTGTGAATGAGTCAATTCAT+AGG - chr5.4:52822104-52822123 None:intergenic 35.0%
TAGGATCCGTACGTTATTAT+TGG + chr5.4:52821081-52821100 MS.gene023746:intron 35.0%
TCACACACAAACACACATAT+AGG - chr5.4:52821714-52821733 None:intergenic 35.0%
TCTACAACTGTGACAAAAAC+AGG - chr5.4:52820976-52820995 None:intergenic 35.0%
TGCGTTAAGATGTGACATAT+GGG + chr5.4:52820539-52820558 MS.gene023746:intron 35.0%
TGGATGAGAATAGCATTTCA+GGG - chr5.4:52819766-52819785 None:intergenic 35.0%
TGGTACTAATGTTCACAAGT+AGG - chr5.4:52822685-52822704 None:intergenic 35.0%
TGGTGTGTTTGTAAACAGAA+AGG + chr5.4:52821101-52821120 MS.gene023746:intron 35.0%
TTATAGGTGCCTTGCTAATT+GGG + chr5.4:52820499-52820518 MS.gene023746:intron 35.0%
TTCAATGCATGCACATTCAA+TGG + chr5.4:52819964-52819983 MS.gene023746:CDS 35.0%
TTGCGTTAAGATGTGACATA+TGG + chr5.4:52820538-52820557 MS.gene023746:intron 35.0%
! AACTTTGAGGATTGGTGAAA+TGG + chr5.4:52820096-52820115 MS.gene023746:CDS 35.0%
! AAGGTATAAGGTTTTCTGGA+TGG - chr5.4:52822024-52822043 None:intergenic 35.0%
! ACTTTGAGGATTGGTGAAAT+GGG + chr5.4:52820097-52820116 MS.gene023746:CDS 35.0%
! ATGTGAAGGCACAACAAATT+AGG - chr5.4:52821745-52821764 None:intergenic 35.0%
! GGGTCACTAGTTTATGAAAA+TGG + chr5.4:52820934-52820953 MS.gene023746:intron 35.0%
! GGTATTGAATTTAGGTGTTG+AGG + chr5.4:52820404-52820423 MS.gene023746:intron 35.0%
! TTTCAGTGTTTTTCCAACAC+CGG + chr5.4:52820445-52820464 MS.gene023746:intron 35.0%
!! AAGATTCGGCATTGATTATC+CGG + chr5.4:52822793-52822812 MS.gene023746:CDS 35.0%
!! ATATGATGATGGTGCTGTTA+AGG - chr5.4:52822648-52822667 None:intergenic 35.0%
!! CTATAGCAAAGACCTTGTTT+TGG - chr5.4:52821243-52821262 None:intergenic 35.0%
!!! CATTTTTTTGGTCTTGAAGG+TGG - chr5.4:52820193-52820212 None:intergenic 35.0%
!!! TTTTTTTTTGGTGATGACCG+GGG - chr5.4:52821574-52821593 None:intergenic 35.0%
AACTAGTGACCCAATTAGCA+AGG - chr5.4:52820926-52820945 None:intergenic 40.0%
AAGTCTTTGCACTTGCTTCT+TGG - chr5.4:52822567-52822586 None:intergenic 40.0%
AATAAGATGATAAGTGGCGG+TGG + chr5.4:52820037-52820056 MS.gene023746:CDS 40.0%
AATCTAGGTGCCTTGCTAAT+TGG + chr5.4:52820913-52820932 MS.gene023746:intron 40.0%
AATGCCGAATCTTGAAGTAG+AGG - chr5.4:52822786-52822805 None:intergenic 40.0%
ACGTTCAGTTGTTAAGGTCT+AGG + chr5.4:52820071-52820090 MS.gene023746:CDS 40.0%
ACTCAACATGCTCAAATTGG+TGG + chr5.4:52819926-52819945 MS.gene023746:CDS 40.0%
AGATAAAGGTGAACCTGATG+AGG + chr5.4:52822895-52822914 MS.gene023746:CDS 40.0%
ATCTAGGTGCCTTGCTAATT+GGG + chr5.4:52820914-52820933 MS.gene023746:intron 40.0%
ATCTAGTTGCCCAATTAGCA+AGG - chr5.4:52820511-52820530 None:intergenic 40.0%
ATCTCAGTGTTTCCAATCTG+AGG - chr5.4:52821824-52821843 None:intergenic 40.0%
ATGAGAATAGCATTTCAGGG+TGG - chr5.4:52819763-52819782 None:intergenic 40.0%
ATGCCGAATCTTGAAGTAGA+GGG - chr5.4:52822785-52822804 None:intergenic 40.0%
ATGTTCACAAGTAGGTTTGG+TGG - chr5.4:52822677-52822696 None:intergenic 40.0%
CAAAGTTGAAAGTTAGGTCG+TGG - chr5.4:52820632-52820651 None:intergenic 40.0%
CGTTCAGTTGTTAAGGTCTA+GGG + chr5.4:52820072-52820091 MS.gene023746:CDS 40.0%
GAACATTACATCGGCCATAT+CGG - chr5.4:52822822-52822841 None:intergenic 40.0%
GGAAGAAATGGCAGTTTCAA+TGG - chr5.4:52822705-52822724 None:intergenic 40.0%
GTTATAGGTGCCTTGCTAAT+TGG + chr5.4:52820498-52820517 MS.gene023746:intron 40.0%
TAAGGTCTAGGGAAACTTTG+AGG + chr5.4:52820083-52820102 MS.gene023746:CDS 40.0%
TAATTTACCTTGGGTGCTTG+CGG - chr5.4:52821901-52821920 None:intergenic 40.0%
TAGCAGCAGGAAGAATGATT+TGG + chr5.4:52821772-52821791 MS.gene023746:intron 40.0%
TAGTAGCAGTGGCAATAGTA+TGG + chr5.4:52822850-52822869 MS.gene023746:CDS 40.0%
TCAATCACACACACATTGTG+AGG - chr5.4:52820894-52820913 None:intergenic 40.0%
TGAACTAGAACTACCCAACA+TGG + chr5.4:52819733-52819752 MS.gene023746:CDS 40.0%
! AAACTGCCATTTCTTCCCAA+GGG + chr5.4:52822708-52822727 MS.gene023746:CDS 40.0%
! ACCCGAGTTGAACATTACAT+CGG - chr5.4:52822831-52822850 None:intergenic 40.0%
! TCTAGGGAAACTTTGAGGAT+TGG + chr5.4:52820088-52820107 MS.gene023746:CDS 40.0%
! TGCGGCCAAGTATTTCTTTT+TGG - chr5.4:52821883-52821902 None:intergenic 40.0%
! TTCAGTGTTTTTCCAACACC+GGG + chr5.4:52820446-52820465 MS.gene023746:intron 40.0%
CAAGTAAAGCCATCTTCTGG+TGG - chr5.4:52821848-52821867 None:intergenic 45.0%
CATGCTCAAATTGGTGGAAG+TGG + chr5.4:52819932-52819951 MS.gene023746:CDS 45.0%
CTCCAAGTAAAGCCATCTTC+TGG - chr5.4:52821851-52821870 None:intergenic 45.0%
GAATAGCATTTCAGGGTGGT+TGG - chr5.4:52819759-52819778 None:intergenic 45.0%
GCCGATGTAATGTTCAACTC+GGG + chr5.4:52822827-52822846 MS.gene023746:CDS 45.0%
GCTGTCTTGTGTGATTGATG+AGG - chr5.4:52821203-52821222 None:intergenic 45.0%
GGACCCTCTACTTCAAGATT+CGG + chr5.4:52822779-52822798 MS.gene023746:CDS 45.0%
GGCCGATGTAATGTTCAACT+CGG + chr5.4:52822826-52822845 MS.gene023746:CDS 45.0%
TGTTGTGCCTTCACATGCAT+TGG + chr5.4:52821749-52821768 MS.gene023746:intron 45.0%
! CACCAGAAGATGGCTTTACT+TGG + chr5.4:52821846-52821865 MS.gene023746:CDS 45.0%
! GAAACTGCCATTTCTTCCCA+AGG + chr5.4:52822707-52822726 MS.gene023746:CDS 45.0%
! TGAGGATTGGTGAAATGGGT+GGG + chr5.4:52820101-52820120 MS.gene023746:CDS 45.0%
! TTGAGGATTGGTGAAATGGG+TGG + chr5.4:52820100-52820119 MS.gene023746:CDS 45.0%
!! ATTCGGCATTGATTATCCGG+TGG + chr5.4:52822796-52822815 MS.gene023746:CDS 45.0%
!! TTCACAAGTAGGTTTGGTGG+TGG - chr5.4:52822674-52822693 None:intergenic 45.0%
ACTGAGATGCCACCAGAAGA+TGG + chr5.4:52821836-52821855 MS.gene023746:CDS 50.0%
ATGATAAGTGGCGGTGGTGA+TGG + chr5.4:52820043-52820062 MS.gene023746:CDS 50.0%
ATGCATCCAGCACCTCAGAT+TGG + chr5.4:52821809-52821828 MS.gene023746:CDS 50.0%
CTTTGCACTTGCTTCTTGGC+TGG - chr5.4:52822563-52822582 None:intergenic 50.0%
GATTGGTGAAATGGGTGGGA+GGG + chr5.4:52820105-52820124 MS.gene023746:CDS 50.0%
GCGGTGACGTTCAGTTGTTA+AGG + chr5.4:52820065-52820084 MS.gene023746:CDS 50.0%
GCTGCTACCAATGCATGTGA+AGG - chr5.4:52821759-52821778 None:intergenic 50.0%
TGATTATCCGGTGGCCGATA+TGG + chr5.4:52822805-52822824 MS.gene023746:CDS 50.0%
TTCAACTCGGGTAGTAGCAG+TGG + chr5.4:52822839-52822858 MS.gene023746:CDS 50.0%
! GTGTTTCCAATCTGAGGTGC+TGG - chr5.4:52821818-52821837 None:intergenic 50.0%
! TCACATGCATTGGTAGCAGC+AGG + chr5.4:52821759-52821778 MS.gene023746:intron 50.0%
!! ACAAGTAGGTTTGGTGGTGG+TGG - chr5.4:52822671-52822690 None:intergenic 50.0%
!! GGTGGTGGTGGATATGATGA+TGG - chr5.4:52822659-52822678 None:intergenic 50.0%
TGAAATGGGTGGGAGGGATG+TGG + chr5.4:52820111-52820130 MS.gene023746:CDS 55.0%
TGGCCACCCTTGGGAAGAAA+TGG - chr5.4:52822717-52822736 None:intergenic 55.0%
! CTGCCATTTCTTCCCAAGGG+TGG + chr5.4:52822711-52822730 MS.gene023746:CDS 55.0%
! GGATTGGTGAAATGGGTGGG+AGG + chr5.4:52820104-52820123 MS.gene023746:CDS 55.0%
! TCAGGGTGGTTGGCCATGTT+GGG - chr5.4:52819749-52819768 None:intergenic 55.0%
! TTCAGGGTGGTTGGCCATGT+TGG - chr5.4:52819750-52819769 None:intergenic 55.0%
!! ATAAGTGGCGGTGGTGATGG+CGG + chr5.4:52820046-52820065 MS.gene023746:CDS 55.0%
ACATCGGCCATATCGGCCAC+CGG - chr5.4:52822815-52822834 None:intergenic 60.0%
ATGGGTGGGAGGGATGTGGA+AGG + chr5.4:52820115-52820134 MS.gene023746:CDS 60.0%
GGGGTTCAAACCTCGAACCC+CGG + chr5.4:52821554-52821573 MS.gene023746:intron 60.0%
GGGGTTCGAGGTTTGAACCC+CGG - chr5.4:52821555-52821574 None:intergenic 60.0%
TACTTGGCCGCAAGCACCCA+AGG + chr5.4:52821891-52821910 MS.gene023746:CDS 60.0%
TGCGGTGAACCACGACCTCA+GGG + chr5.4:52822739-52822758 MS.gene023746:CDS 60.0%
!! TTGGTGATGACCGGGGTTCG+AGG - chr5.4:52821567-52821586 None:intergenic 60.0%
AGGTCGTGGTTCACCGCAGG+TGG - chr5.4:52822737-52822756 None:intergenic 65.0%
CTGAGGTCGTGGTTCACCGC+AGG - chr5.4:52822740-52822759 None:intergenic 65.0%
CTGCGGTGAACCACGACCTC+AGG + chr5.4:52822738-52822757 MS.gene023746:CDS 65.0%
GCGGTGAACCACGACCTCAG+GGG + chr5.4:52822740-52822759 MS.gene023746:CDS 65.0%
GTGAACCACGACCTCAGGGG+CGG + chr5.4:52822743-52822762 MS.gene023746:CDS 65.0%
TTCCCAAGGGTGGCCACCTG+CGG + chr5.4:52822721-52822740 MS.gene023746:CDS 65.0%
CACCGCAGGTGGCCACCCTT+GGG - chr5.4:52822726-52822745 None:intergenic 70.0%
CACGACCTCAGGGGCGGAGA+CGG + chr5.4:52822749-52822768 MS.gene023746:CDS 70.0%
CAGTACCGTCTCCGCCCCTG+AGG - chr5.4:52822757-52822776 None:intergenic 70.0%
CTCAGGGGCGGAGACGGTAC+TGG + chr5.4:52822755-52822774 MS.gene023746:CDS 70.0%
TCACCGCAGGTGGCCACCCT+TGG - chr5.4:52822727-52822746 None:intergenic 70.0%
! AGGGGCGGAGACGGTACTGG+TGG + chr5.4:52822758-52822777 MS.gene023746:CDS 70.0%
CGTCTCCGCCCCTGAGGTCG+TGG - chr5.4:52822751-52822770 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 52819680 52822928 52819680 ID=MS.gene023746
chr5.4 mRNA 52819680 52822928 52819680 ID=MS.gene023746.t1;Parent=MS.gene023746
chr5.4 exon 52819680 52820212 52819680 ID=MS.gene023746.t1.exon1;Parent=MS.gene023746.t1
chr5.4 CDS 52819680 52820212 52819680 ID=cds.MS.gene023746.t1;Parent=MS.gene023746.t1
chr5.4 exon 52821775 52821912 52821775 ID=MS.gene023746.t1.exon2;Parent=MS.gene023746.t1
chr5.4 CDS 52821775 52821912 52821775 ID=cds.MS.gene023746.t1;Parent=MS.gene023746.t1
chr5.4 exon 52822520 52822928 52822520 ID=MS.gene023746.t1.exon3;Parent=MS.gene023746.t1
chr5.4 CDS 52822520 52822928 52822520 ID=cds.MS.gene023746.t1;Parent=MS.gene023746.t1
Gene Sequence

>MS.gene023746

ATGGAAGAAGTCATGAATTTCATCATGCGTGCTTTTGAATTAGCAAAAAATCTTGAACTAGAACTACCCAACATGGCCAACCACCCTGAAATGCTATTCTCATCCATTGATGATGTAGAAAAAGCATTTAATGCTGCCAAAGAAAGAGTAATGATGATGATGTCATCACAACATGACTCAACAACAACAACAAAACCTTCTAATTCCTTTGCTCAAATTCTGTCTCATGATGTTTTCATGCAAAAAACTCAACATGCTCAAATTGGTGGAAGTGGAAGTGCAATTTCAATGCATGCACATTCAATGGATCAATTGCTTCTAATGCAACACCCTTTTGATGTGAGTGTTCTAATTGAGAATAAGATGATAAGTGGCGGTGGTGATGGCGGTGACGTTCAGTTGTTAAGGTCTAGGGAAACTTTGAGGATTGGTGAAATGGGTGGGAGGGATGTGGAAGGTTTAGATAGATCAAAAGGGTTAGAAGGAGATCAAATGCAAGCAATAGAAACACCACCTTCAAGACCAAAAAAATGGTATGTGTATAATACATTTATGTTTATAAGTTCTTTATTATTAATTAGTTGATTAATTAAAGTGAATATGTATATCAAAGTTTCATATATTAAATGGAGCTTGACATAGCCTCACAATCTATGTTATTGAATCAAAGTCTTGGAATTGATTGAAATGAAACATCTTGAAACAACACAAAGATTATTTCTAAGGTATTGAATTTAGGTGTTGAGGTTGATTGAATTAATCTAGTTTCAGTGTTTTTCCAACACCGGGTAATTTACATATTTGGGTTTTAGTTTATGGTTATAGGTGCCTTGCTAATTGGGCAACTAGATTACAAAATTGCGTTAAGATGTGACATATGGGACCTTAATTTCAGTTGAATTGCAATCATAGATACATAAATAATCTTGATGTTGTGTCTATGTCGCAGCCACGACCTAACTTTCAACTTTGATGTATACATGTGATTGAAAGTACTAGCTAGCTTATGATGAGAACAAATCTTTTCTGTAAGATTTTCACTAGAGGGAGTCCATAATGAAAATTTTCACCCTACTTAGGATTTTCACATTATAAAACTTTTAATCAAATCATACCAATCATTTCCATAAGATTTTTACTAGAGGGAGTCGAAGTTCCTAGTACAAACATTGAGTAATCTAAAAGTAGAGATTGAAATGAGGTTAACTTGGCCTCACAATGTGTGTGTGATTGAATCTAGGTGCCTTGCTAATTGGGTCACTAGTTTATGAAAATGGTGAAGATAGAGCTTGTCCTGTTTTTGTCACAGTTGTAGATTGCTTACACTTCAGCCGCCAACAACAATAATTGAAAGAAAGAAAACAAAAGTGAAATGCTTTTGAGGTATTGAAAGCTTTAGTTTAGGATCCGTACGTTATTATTGGTGTGTTTGTAAACAGAAAGGAAACTGACACAAAATGTAAGATAGAATTTTCCATGGTCTTTCTTAGTAATTAGGCAACAAGTCATCATGGATATTTCCTCATCAATCACACAAGACAGCTGTGTTAGTTTACATGACCAAAACAAGGTCTTTGCTATAGTATTTAGGCACAAATAGACATTTTCTCTAACCATAAAAAAAAAATTGATTTGAATTTTTTTGGTGTTTGCTATTTTGGTGCGTGTCTAAGAACTGTGTATTTGGTCTTGTGATCATGTAAGACTAGCTTCACTTAAACATATTGAATTGAAAGTTCTTATAAATTATATCAAATTTATATACATTTCATGCACAAAAATAAACTTGTTGAAATTGTATCTCACTAGTTAATTATTTTAATGGATTATATGATATTATATTATATATGCACAAATTTATATATGCAAGATCCGGGGTTCAAACCTCGAACCCCGGTCATCACCAAAAAAAAAATTCACTTTACTACACAATAGTTTGACACAATTATTAGGAAGTTAATATGGTATAATTCAGTTGTTAGATTGGTCAATTTTTTACATAAAATTATAAGTAGGTTAATTGATCATTTTCCTATATGTGTGTTTGTGTGTGACACATTGACCTAATTTGTTGTGCCTTCACATGCATTGGTAGCAGCAGGAAGAATGATTTGGAGAAGAGAACAGAGATGCATCCAGCACCTCAGATTGGAAACACTGAGATGCCACCAGAAGATGGCTTTACTTGGAGAAAATATGGCCAAAAAGAAATACTTGGCCGCAAGCACCCAAGGTAAATTAACAATAATTACTTAGAATTGTACTTTATAATATAGGGTAATAATTTCCATAAAGAGTTTGAAAGTTTCAATTCTAAGACAAATTCGTCTTTCATTATGCACCATCCAGAAAACCTTATACCTTTCTTCTAAGAATATTTCTTGGTCATGTTGAAAATTCAGTAGAACAACAAGTGTGTGCCCTATGAATTGACTCATTCACACATCATAACAAGACAAGTTGACTATTTACATTCATGTATAAGGGACAACTAGTCGCTTTTTCATCACAACTACTCAACTAGTATTAAAAAAAGAAAATACTAAGATGAATAAATTCATCACGTTTATTTTGCCTTGATTACTTGTTAAATATTTCTTTTTCTTTTTTTTAATAATTGATTAGCATTAAAAAAAAAAAAAAAATCTAAAACAGCAGTTTTGAATGAGAAATTGTCTTGAGTAGCTTATCTAAAAAAAATATTTTCAATAATTGATTTTCTTCAAAAAAAAAAATTGATTTCATTATAGTAAGCAACACTTAATTGTTTATTTTTTAAACATCTTGGTTTTTATTATATAATTTGTTGGCTAATTTGAAATATTTATTTCCTTTATCAGAAGTTACTATAGGTGCACACACCAAAAATTATATTTGTGTCCAGCCAAGAAGCAAGTGCAAAGACTTGATGAAAACCCTAACATAGTTGAAGTAACATATAGAGGTGAACACATTTGTCACAAATCCTTAACAGCACCATCATCATATCCACCACCACCAAACCTACTTGTGAACATTAGTACCATTGAAACTGCCATTTCTTCCCAAGGGTGGCCACCTGCGGTGAACCACGACCTCAGGGGCGGAGACGGTACTGGTGGACCCTCTACTTCAAGATTCGGCATTGATTATCCGGTGGCCGATATGGCCGATGTAATGTTCAACTCGGGTAGTAGCAGTGGCAATAGTATGGAATCACTCTTTTATCTAACTGAAGATAAAGGTGAACCTGATGAGGAGAAAAACTGA
Protein sequence

>MS.gene023746.t1

MEEVMNFIMRAFELAKNLELELPNMANHPEMLFSSIDDVEKAFNAAKERVMMMMSSQHDSTTTTKPSNSFAQILSHDVFMQKTQHAQIGGSGSAISMHAHSMDQLLLMQHPFDVSVLIENKMISGGGDGGDVQLLRSRETLRIGEMGGRDVEGLDRSKGLEGDQMQAIETPPSRPKKCSRKNDLEKRTEMHPAPQIGNTEMPPEDGFTWRKYGQKEILGRKHPRSYYRCTHQKLYLCPAKKQVQRLDENPNIVEVTYRGEHICHKSLTAPSSYPPPPNLLVNISTIETAISSQGWPPAVNHDLRGGDGTGGPSTSRFGIDYPVADMADVMFNSGSSSGNSMESLFYLTEDKGEPDEEKN