AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023843


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023843.t1 MTR_7g076740 96.296 216 8 0 11 226 89 304 2.55e-152 425
MS.gene023843.t1 MTR_8g031360 61.611 211 74 5 11 218 90 296 1.32e-76 233
MS.gene023843.t1 MTR_1g100653 41.892 222 92 10 11 221 88 283 8.15e-37 130
MS.gene023843.t1 MTR_7g011170 62.500 80 29 1 11 90 88 166 7.07e-26 102
MS.gene023843.t1 MTR_6g012690 63.415 82 28 1 11 90 88 169 1.44e-25 102
MS.gene023843.t1 MTR_4g082290 60.000 85 32 1 11 93 88 172 4.15e-25 102
MS.gene023843.t1 MTR_4g082230 59.302 86 33 1 10 93 87 172 4.41e-25 102
MS.gene023843.t1 MTR_5g042030 50.000 76 33 1 10 85 82 152 2.83e-18 81.6
MS.gene023843.t1 MTR_5g007370 76.190 42 10 0 10 51 87 128 4.46e-17 78.6
MS.gene023843.t1 MTR_3g074520 70.213 47 14 0 11 57 88 134 4.96e-17 79.0
MS.gene023843.t1 MTR_2g011660 73.810 42 11 0 10 51 88 129 1.62e-16 77.4
MS.gene023843.t1 MTR_5g078140 70.455 44 13 0 10 53 87 130 2.91e-16 76.6
MS.gene023843.t1 MTR_4g478180 73.810 42 11 0 10 51 88 129 2.99e-16 76.6
MS.gene023843.t1 MTR_1g112760 69.565 46 14 0 7 52 85 130 3.68e-16 75.1
MS.gene023843.t1 MTR_8g095390 66.667 45 15 0 11 55 88 132 3.83e-16 76.3
MS.gene023843.t1 MTR_4g091490 73.171 41 11 0 11 51 89 129 4.22e-16 76.3
MS.gene023843.t1 MTR_4g105130 45.349 86 45 1 11 96 88 171 7.27e-16 75.9
MS.gene023843.t1 MTR_6g009430 56.863 51 22 0 7 57 84 134 1.83e-15 74.3
MS.gene023843.t1 MTR_4g102380 69.767 43 13 0 10 52 87 129 2.77e-15 73.2
MS.gene023843.t1 MTR_2g089620 71.429 42 12 0 10 51 87 128 2.81e-15 73.9
MS.gene023843.t1 MTR_3g077110 68.889 45 14 0 7 51 84 128 3.14e-15 73.9
MS.gene023843.t1 MTR_3g045430 61.224 49 19 0 7 55 85 133 7.09e-15 72.4
MS.gene023843.t1 MTR_1g085040 64.444 45 16 0 11 55 88 132 8.80e-15 72.8
MS.gene023843.t1 MTR_1g085640 64.444 45 16 0 11 55 88 132 1.05e-14 72.4
MS.gene023843.t1 MTR_4g073420 43.243 74 37 1 11 84 88 156 1.22e-14 71.6
MS.gene023843.t1 MTR_3g083540 62.791 43 16 0 10 52 116 158 2.42e-14 70.9
MS.gene023843.t1 MTR_3g097450 65.854 41 14 0 10 50 87 127 2.72e-14 70.9
MS.gene023843.t1 MTR_3g065440 70.732 41 12 0 10 50 87 127 2.78e-14 70.9
MS.gene023843.t1 MTR_5g029840 64.286 42 15 0 10 51 87 128 2.86e-14 70.9
MS.gene023843.t1 MTR_7g110830 62.222 45 17 0 11 55 88 132 3.53e-14 70.9
MS.gene023843.t1 MTR_3g461490 59.574 47 19 0 11 57 88 134 4.76e-14 70.5
MS.gene023843.t1 MTR_5g079670 56.098 41 18 0 10 50 86 126 9.37e-13 65.5
MS.gene023843.t1 MTR_2g064160 59.184 49 18 1 11 59 87 133 1.46e-12 65.9
MS.gene023843.t1 MTR_4g485530 60.000 40 16 0 10 49 87 126 4.00e-12 63.2
MS.gene023843.t1 MTR_5g007310 64.103 39 14 0 21 59 6 44 1.89e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023843.t1 AT3G61250 50.235 213 78 11 11 218 88 277 5.06e-52 170
MS.gene023843.t1 AT3G01140 56.989 93 35 1 11 98 88 180 1.26e-26 105
MS.gene023843.t1 AT3G01140 56.989 93 35 1 11 98 131 223 1.90e-26 105
MS.gene023843.t1 AT5G15310 63.415 82 26 2 11 90 9 88 1.84e-25 100
MS.gene023843.t1 AT5G15310 63.415 82 26 2 11 90 88 167 1.17e-24 98.2
MS.gene023843.t1 AT5G15310 63.415 82 26 2 11 90 88 167 1.58e-24 99.4
MS.gene023843.t1 AT5G15310 63.415 82 26 2 11 90 88 167 1.67e-24 99.0
MS.gene023843.t1 AT5G10280 43.750 112 37 4 11 100 88 195 6.49e-17 78.6
MS.gene023843.t1 AT5G61420 72.727 44 12 0 11 54 9 52 1.68e-16 77.0
MS.gene023843.t1 AT4G21440 67.391 46 15 0 10 55 87 132 3.02e-16 76.6
MS.gene023843.t1 AT4G05100 71.429 42 12 0 10 51 88 129 4.46e-16 76.3
MS.gene023843.t1 AT1G34670 38.971 136 66 5 11 131 88 221 5.27e-16 76.3
MS.gene023843.t1 AT5G65230 75.610 41 10 0 11 51 88 128 5.99e-16 75.5
MS.gene023843.t1 AT5G61420 72.727 44 12 0 11 54 88 131 7.52e-16 75.9
MS.gene023843.t1 AT1G22640 44.286 70 39 0 3 72 8 77 8.20e-16 73.2
MS.gene023843.t1 AT4G28110 70.732 41 12 0 11 51 4 44 1.67e-15 72.4
MS.gene023843.t1 AT1G66230 65.909 44 15 0 10 53 87 130 1.74e-15 73.9
MS.gene023843.t1 AT4G22680 69.767 43 13 0 10 52 87 129 1.88e-15 73.6
MS.gene023843.t1 AT5G16600 65.909 44 15 0 10 53 87 130 2.21e-15 74.3
MS.gene023843.t1 AT4G17785 70.732 41 12 0 11 51 89 129 2.34e-15 74.3
MS.gene023843.t1 AT1G22640 50.000 62 31 0 11 72 31 92 2.35e-15 72.4
MS.gene023843.t1 AT4G17785 70.732 41 12 0 11 51 89 129 2.53e-15 74.3
MS.gene023843.t1 AT1G18570 63.462 52 19 0 10 61 88 139 3.06e-15 73.9
MS.gene023843.t1 AT5G16770 69.048 42 13 0 10 51 87 128 3.66e-15 73.6
MS.gene023843.t1 AT5G16770 69.048 42 13 0 10 51 87 128 3.66e-15 73.6
MS.gene023843.t1 AT5G07700 68.000 50 14 1 11 60 88 135 4.28e-15 73.6
MS.gene023843.t1 AT5G07700 68.000 50 14 1 11 60 88 135 4.28e-15 73.6
MS.gene023843.t1 AT5G54230 67.391 46 15 0 10 55 87 132 4.47e-15 73.2
MS.gene023843.t1 AT5G16770 69.048 42 13 0 10 51 87 128 4.90e-15 73.2
MS.gene023843.t1 AT5G16770 69.048 42 13 0 10 51 87 128 4.90e-15 73.2
MS.gene023843.t1 AT1G09540 60.417 48 19 0 11 58 88 135 5.24e-15 73.6
MS.gene023843.t1 AT3G02940 68.293 41 13 0 11 51 88 128 7.09e-15 72.4
MS.gene023843.t1 AT5G60890 72.727 44 12 0 11 54 88 131 7.15e-15 72.4
MS.gene023843.t1 AT3G13540 64.286 42 15 0 11 52 99 140 7.67e-15 71.6
MS.gene023843.t1 AT4G34990 65.116 43 15 0 10 52 87 129 8.54e-15 72.0
MS.gene023843.t1 AT3G02940 68.293 41 13 0 11 51 88 128 8.68e-15 72.4
MS.gene023843.t1 AT3G02940 68.293 41 13 0 11 51 88 128 8.68e-15 72.4
MS.gene023843.t1 AT1G22640 50.000 62 31 0 11 72 88 149 8.84e-15 71.6
MS.gene023843.t1 AT4G09460 66.667 42 14 0 11 52 88 129 1.01e-14 71.2
MS.gene023843.t1 AT4G28110 73.171 41 11 0 11 51 88 128 1.09e-14 71.6
MS.gene023843.t1 AT5G26660 60.417 48 19 0 7 54 53 100 1.13e-14 72.0
MS.gene023843.t1 AT1G35515 66.667 42 14 0 11 52 88 129 1.34e-14 70.5
MS.gene023843.t1 AT5G56110 58.333 48 20 0 10 57 87 134 1.34e-14 72.0
MS.gene023843.t1 AT5G26660 60.417 48 19 0 7 54 84 131 1.90e-14 71.6
MS.gene023843.t1 AT1G74430 64.444 45 16 0 10 54 18 62 2.38e-14 69.7
MS.gene023843.t1 AT2G16720 65.116 43 15 0 10 52 87 129 3.27e-14 70.1
MS.gene023843.t1 AT5G07690 58.929 56 22 1 11 66 88 142 3.27e-14 70.9
MS.gene023843.t1 AT4G38620 61.905 42 16 0 11 52 88 129 3.76e-14 70.1
MS.gene023843.t1 AT4G12350 62.791 43 16 0 10 52 87 129 1.19e-13 68.9
MS.gene023843.t1 AT5G14340 58.696 46 19 0 11 56 10 55 1.30e-13 67.0
MS.gene023843.t1 AT1G74430 65.909 44 15 0 11 54 88 131 2.60e-13 67.8
MS.gene023843.t1 AT4G01680 52.830 53 25 0 11 63 88 140 4.32e-13 66.6
MS.gene023843.t1 AT5G62320 62.500 40 15 0 10 49 95 134 4.76e-13 66.6
MS.gene023843.t1 AT5G14340 57.447 47 20 0 10 56 99 145 8.30e-13 66.2
MS.gene023843.t1 AT4G01680 52.830 53 25 0 11 63 88 140 1.05e-12 66.6
MS.gene023843.t1 AT4G01680 52.830 53 25 0 11 63 100 152 1.07e-12 66.6
MS.gene023843.t1 AT1G57560 54.000 50 23 0 11 60 88 137 1.12e-12 66.2
MS.gene023843.t1 AT1G57560 60.465 43 17 0 11 53 88 130 1.57e-12 65.5
MS.gene023843.t1 AT3G12720 69.231 39 12 0 11 49 98 136 3.88e-12 64.7
MS.gene023843.t1 AT3G28470 39.130 115 51 3 7 109 84 191 4.27e-12 64.7
MS.gene023843.t1 AT1G18710 61.905 42 16 0 11 52 88 129 1.51e-11 62.8
MS.gene023843.t1 AT1G74080 67.500 40 13 0 11 50 88 127 2.75e-11 62.4
MS.gene023843.t1 AT1G18960 50.000 50 25 0 6 55 76 125 4.15e-11 62.0

Find 34 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATTACTTTCTCCGTATA+TGG 0.178709 7.1:+26462432 MS.gene023843:CDS
GATGCGTGTGACTTGTTATT+TGG 0.197120 7.1:-26462287 None:intergenic
TATCTCATTGTCTGTTCTTC+CGG 0.226667 7.1:-26462171 None:intergenic
AGTAGTGACAAATGCCTAAA+AGG 0.245360 7.1:-26462786 None:intergenic
GATAATGATCCTGCAGAAAT+TGG 0.270588 7.1:-26462539 None:intergenic
ATCTCATTGTCTGTTCTTCC+GGG 0.293138 7.1:-26462170 None:intergenic
ACAATGAGATAAAGAACTTT+TGG 0.296577 7.1:+26462183 MS.gene023843:CDS
ATGTTAGTATGATCTGTATC+TGG 0.314171 7.1:-26462494 None:intergenic
AACAATGACATGAGCTTCTT+AGG 0.316806 7.1:+26462746 MS.gene023843:CDS
CAACTTCAGAGTTCCATATA+CGG 0.425073 7.1:-26462445 None:intergenic
AAGCTCATGTCATTGTTCAT+AGG 0.447397 7.1:-26462740 None:intergenic
ATGCGTGTGACTTGTTATTT+GGG 0.448476 7.1:-26462286 None:intergenic
AACACGTCACATGGCTCAAT+GGG 0.449186 7.1:+26462316 MS.gene023843:CDS
AGAAATGGAGTCACATTTGT+TGG 0.449246 7.1:-26462564 None:intergenic
CGTATATGGAACTCTGAAGT+TGG 0.454090 7.1:+26462446 MS.gene023843:CDS
GCTCGCTGTAACAGCAGAAA+TGG 0.457228 7.1:-26462579 None:intergenic
TGATGAATCTTGCAAGTCAT+TGG 0.458871 7.1:-26462687 None:intergenic
GAAGAAACGTCTCAAAAGCC+TGG 0.462496 7.1:+26462217 MS.gene023843:CDS
TGTCGCTGCAACTGCGGTCG+AGG 0.476272 7.1:-26461942 None:intergenic
CAACACGTCACATGGCTCAA+TGG 0.478902 7.1:+26462315 MS.gene023843:CDS
GTAGTGACAAATGCCTAAAA+GGG 0.479751 7.1:-26462785 None:intergenic
TTGTGAAAGAAGAGTTGGAT+TGG 0.480337 7.1:+26462609 MS.gene023843:CDS
TAGTATTGTGAAAGAAGAGT+TGG 0.500514 7.1:+26462604 MS.gene023843:CDS
TGTCAGAGTCCAATTTCTGC+AGG 0.508361 7.1:+26462530 MS.gene023843:CDS
ATTTGGGTAACTAGTTAATG+AGG 0.514248 7.1:-26462270 None:intergenic
TGGCTCAATGGGAGAGTGCA+AGG 0.542923 7.1:+26462327 MS.gene023843:CDS
CTTTGATGTCGCTGCAACTG+CGG 0.544610 7.1:-26461948 None:intergenic
AAGAAACGTCTCAAAAGCCT+GGG 0.574138 7.1:+26462218 MS.gene023843:CDS
ATCGGTTTCAACACGTCACA+TGG 0.583488 7.1:+26462307 MS.gene023843:CDS
TAACTAGTTAATGAGGCTAC+CGG 0.584460 7.1:-26462263 None:intergenic
AAATAACAAGTCACACGCAT+CGG 0.590420 7.1:+26462289 MS.gene023843:CDS
GCAATTGCATCTCAGTTACC+CGG 0.621059 7.1:+26462152 MS.gene023843:CDS
AGTTGGAGATGCATTTCGCA+AGG 0.630719 7.1:+26462463 MS.gene023843:CDS
TATACGGAGAAAGTAATCAG+AGG 0.679714 7.1:-26462429 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAATTTAACAATATTTC+AGG + chr7.1:26462123-26462142 MS.gene023843:intron 15.0%
!! AAATTTAACAATATTTCAGG+TGG + chr7.1:26462126-26462145 MS.gene023843:intron 20.0%
!! ATAAAAGTTTGAACTGATTA+AGG - chr7.1:26462066-26462085 None:intergenic 20.0%
!! GATCATTAAATCTAGATAAT+TGG + chr7.1:26462026-26462045 MS.gene023843:intron 20.0%
!! TTATCTAGATTTAATGATCA+TGG - chr7.1:26462025-26462044 None:intergenic 20.0%
!!! AATTTAACAATATTTCAGGT+GGG + chr7.1:26462127-26462146 MS.gene023843:intron 20.0%
!! ACAATGAGATAAAGAACTTT+TGG + chr7.1:26462183-26462202 MS.gene023843:CDS 25.0%
!! TCAATTTTTGTGAAGGAATT+AGG - chr7.1:26462404-26462423 None:intergenic 25.0%
!! TTAATGATCATGGATTTTTG+AGG - chr7.1:26462015-26462034 None:intergenic 25.0%
ATGTTAGTATGATCTGTATC+TGG - chr7.1:26462497-26462516 None:intergenic 30.0%
ATTTGGGTAACTAGTTAATG+AGG - chr7.1:26462273-26462292 None:intergenic 30.0%
TAGTATTGTGAAAGAAGAGT+TGG + chr7.1:26462604-26462623 MS.gene023843:CDS 30.0%
! GGAAGAATCAATTTTTGTGA+AGG - chr7.1:26462411-26462430 None:intergenic 30.0%
AAAAAGTCCAAATGCCATCT+TGG - chr7.1:26461989-26462008 None:intergenic 35.0%
AAATAACAAGTCACACGCAT+CGG + chr7.1:26462289-26462308 MS.gene023843:CDS 35.0%
AACAATGACATGAGCTTCTT+AGG + chr7.1:26462746-26462765 MS.gene023843:CDS 35.0%
AAGCTCATGTCATTGTTCAT+AGG - chr7.1:26462743-26462762 None:intergenic 35.0%
AGAAATGGAGTCACATTTGT+TGG - chr7.1:26462567-26462586 None:intergenic 35.0%
CAACTTCAGAGTTCCATATA+CGG - chr7.1:26462448-26462467 None:intergenic 35.0%
CTGATTACTTTCTCCGTATA+TGG + chr7.1:26462432-26462451 MS.gene023843:CDS 35.0%
GATAATGATCCTGCAGAAAT+TGG - chr7.1:26462542-26462561 None:intergenic 35.0%
TAACTAGTTAATGAGGCTAC+CGG - chr7.1:26462266-26462285 None:intergenic 35.0%
TATACGGAGAAAGTAATCAG+AGG - chr7.1:26462432-26462451 None:intergenic 35.0%
TATCTCATTGTCTGTTCTTC+CGG - chr7.1:26462174-26462193 None:intergenic 35.0%
TGATGAATCTTGCAAGTCAT+TGG - chr7.1:26462690-26462709 None:intergenic 35.0%
TTGTGAAAGAAGAGTTGGAT+TGG + chr7.1:26462609-26462628 MS.gene023843:CDS 35.0%
!! ATGCGTGTGACTTGTTATTT+GGG - chr7.1:26462289-26462308 None:intergenic 35.0%
AAGAAACGTCTCAAAAGCCT+GGG + chr7.1:26462218-26462237 MS.gene023843:CDS 40.0%
ATCTCATTGTCTGTTCTTCC+GGG - chr7.1:26462173-26462192 None:intergenic 40.0%
ATTTGATGTCGTGACCAAGA+TGG + chr7.1:26461972-26461991 MS.gene023843:intron 40.0%
TGATCCAAAAACACATGAGC+CGG + chr7.1:26462244-26462263 MS.gene023843:CDS 40.0%
!! CGTATATGGAACTCTGAAGT+TGG + chr7.1:26462446-26462465 MS.gene023843:CDS 40.0%
!! GATGCGTGTGACTTGTTATT+TGG - chr7.1:26462290-26462309 None:intergenic 40.0%
!!! GTGTTTTTGGATCAATTCCC+AGG - chr7.1:26462238-26462257 None:intergenic 40.0%
AACACGTCACATGGCTCAAT+GGG + chr7.1:26462316-26462335 MS.gene023843:CDS 45.0%
AGTTGGAGATGCATTTCGCA+AGG + chr7.1:26462463-26462482 MS.gene023843:CDS 45.0%
GAAGAAACGTCTCAAAAGCC+TGG + chr7.1:26462217-26462236 MS.gene023843:CDS 45.0%
GCAATTGCATCTCAGTTACC+CGG + chr7.1:26462152-26462171 MS.gene023843:CDS 45.0%
TGTCAGAGTCCAATTTCTGC+AGG + chr7.1:26462530-26462549 MS.gene023843:CDS 45.0%
! ATCGGTTTCAACACGTCACA+TGG + chr7.1:26462307-26462326 MS.gene023843:CDS 45.0%
CAACACGTCACATGGCTCAA+TGG + chr7.1:26462315-26462334 MS.gene023843:CDS 50.0%
CTTTGATGTCGCTGCAACTG+CGG - chr7.1:26461951-26461970 None:intergenic 50.0%
GCTCGCTGTAACAGCAGAAA+TGG - chr7.1:26462582-26462601 None:intergenic 50.0%
GTCGTGACCAAGATGGCATT+TGG + chr7.1:26461979-26461998 MS.gene023843:intron 50.0%
!!! GCTACCGGCTCATGTGTTTT+TGG - chr7.1:26462251-26462270 None:intergenic 50.0%
! TGGCTCAATGGGAGAGTGCA+AGG + chr7.1:26462327-26462346 MS.gene023843:CDS 55.0%
TGTCGCTGCAACTGCGGTCG+AGG - chr7.1:26461945-26461964 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 26461938 26462793 26461938 ID=MS.gene023843
chr7.1 mRNA 26461938 26462793 26461938 ID=MS.gene023843.t1;Parent=MS.gene023843
chr7.1 exon 26461938 26461969 26461938 ID=MS.gene023843.t1.exon1;Parent=MS.gene023843.t1
chr7.1 CDS 26461938 26461969 26461938 ID=cds.MS.gene023843.t1;Parent=MS.gene023843.t1
chr7.1 exon 26462145 26462793 26462145 ID=MS.gene023843.t1.exon2;Parent=MS.gene023843.t1
chr7.1 CDS 26462145 26462793 26462145 ID=cds.MS.gene023843.t1;Parent=MS.gene023843.t1
Gene Sequence

>MS.gene023843

ATGTCCTCGACCGCAGTTGCAGCGACATCAAAGTATTTGATGTCGTGACCAAGATGGCATTTGGACTTTTTTTTCCTCAAAAATCCATGATCATTAAATCTAGATAATTGGATGCATTCTTTGAACCTTAATCAGTTCAAACTTTTATTTTGAGTTCATTAACTAATAGCTTACAACAATATTTCAACAAATTTAACAATATTTCAGGTGGGCTGCAATTGCATCTCAGTTACCCGGAAGAACAGACAATGAGATAAAGAACTTTTGGAACACGCATTTGAAGAAACGTCTCAAAAGCCTGGGAATTGATCCAAAAACACATGAGCCGGTAGCCTCATTAACTAGTTACCCAAATAACAAGTCACACGCATCGGTTTCAACACGTCACATGGCTCAATGGGAGAGTGCAAGGCTTGAAGCAGAAGCAAGACTCTCAATTGAGTCATCACACTTCAACAACAATCCTAATTCCTTCACAAAAATTGATTCTTCCTCTGATTACTTTCTCCGTATATGGAACTCTGAAGTTGGAGATGCATTTCGCAAGGTTCAAAAACCAGATACAGATCATACTAACATAATCACTAGTTGCTGTCAGAGTCCAATTTCTGCAGGATCATTATCTTCCAACAAATGTGACTCCATTTCTGCTGTTACAGCGAGCAATAGTATTGTGAAAGAAGAGTTGGATTGGAAGAACTACAAGTTTTGTGCTGAAGATGTGATTCTTGAATCTGATTCATCAAGCTCCAATGACTTGCAAGATTCATCAGACACTGCTTTGCAGCTCTTGTTTGATTTTCCTATGAACAATGACATGAGCTTCTTAGGAAACTCTTTTGCTTCTCCCTTTTAG

Protein sequence

>MS.gene023843.t1

MSSTAVAATSKWAAIASQLPGRTDNEIKNFWNTHLKKRLKSLGIDPKTHEPVASLTSYPNNKSHASVSTRHMAQWESARLEAEARLSIESSHFNNNPNSFTKIDSSSDYFLRIWNSEVGDAFRKVQKPDTDHTNIITSCCQSPISAGSLSSNKCDSISAVTASNSIVKEELDWKNYKFCAEDVILESDSSSSNDLQDSSDTALQLLFDFPMNNDMSFLGNSFASPF