AlfalfaGEDB | MS.gene02388

M. sativa cultivar XinJiangDaYe / MS.gene02388

KEGG: K08066
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02388.t1	XP_013462829.1	77.4	265	36	4	1	262	1	244	9.60E-100	373.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02388.t1	Q9SMP0	49.1	163	74	2	74	236	46	199	1.9e-32	141.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02388.t1	I3TAY7	77.4	265	36	4	1	262	1	244	6.9e-100	373.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene02388.t1	TF	NF-YC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02388.t1	MTR_2g023340	77.358	265	36	4	1	262	1	244	9.33e-133	375
MS.gene02388.t1	MTR_7g113680	48.387	186	83	6	75	256	134	310	4.47e-50	167
MS.gene02388.t1	MTR_1g082660	56.391	133	55	3	75	207	83	212	1.46e-44	151
MS.gene02388.t1	MTR_3g099180	50.000	150	59	2	74	223	36	169	2.24e-40	139
MS.gene02388.t1	MTR_3g099180	50.000	150	59	2	74	223	36	169	2.54e-40	139
MS.gene02388.t1	MTR_2g081600	49.020	102	48	2	70	170	2	100	2.38e-23	92.0
MS.gene02388.t1	MTR_2g081630	49.505	101	47	2	70	169	2	99	2.58e-23	92.0
MS.gene02388.t1	MTR_3g012030	42.308	78	45	0	94	171	9	86	6.78e-12	64.7
MS.gene02388.t1	MTR_8g041420	39.744	78	47	0	94	171	9	86	2.48e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02388.t1	AT1G54830	49.444	180	71	7	75	251	52	214	3.86e-45	151
MS.gene02388.t1	AT1G54830	49.444	180	71	7	75	251	52	214	3.86e-45	151
MS.gene02388.t1	AT1G54830	49.444	180	71	7	75	251	52	214	3.86e-45	151
MS.gene02388.t1	AT1G56170	57.812	128	49	3	72	197	55	179	2.35e-44	149
MS.gene02388.t1	AT1G56170	57.812	128	49	3	72	197	55	179	2.35e-44	149
MS.gene02388.t1	AT3G48590	46.629	178	86	3	75	252	47	215	1.15e-43	148
MS.gene02388.t1	AT1G08970	61.983	121	39	2	75	195	62	175	4.25e-43	146
MS.gene02388.t1	AT1G08970	61.983	121	39	2	75	195	62	175	4.25e-43	146
MS.gene02388.t1	AT1G08970	61.983	121	39	2	75	195	62	175	4.25e-43	146
MS.gene02388.t1	AT1G08970	61.983	121	39	2	75	195	62	175	4.25e-43	146
MS.gene02388.t1	AT5G63470	48.795	166	73	3	74	236	59	215	2.90e-42	145
MS.gene02388.t1	AT5G63470	48.795	166	73	3	74	236	59	215	2.90e-42	145
MS.gene02388.t1	AT5G50480	54.783	115	46	3	88	199	49	160	9.74e-35	124
MS.gene02388.t1	AT5G27910	47.458	118	50	2	82	199	25	130	2.46e-27	104
MS.gene02388.t1	AT5G50490	43.860	114	59	1	60	173	8	116	3.16e-24	96.3
MS.gene02388.t1	AT5G50470	43.966	116	59	1	88	197	58	173	4.20e-24	96.7
MS.gene02388.t1	AT5G38140	38.835	103	61	2	68	170	44	144	1.05e-19	84.7

Find 59 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTTCTTAATCCTTGTTAA+AGG	0.176954	2.2:-62304930	None:intergenic
ATTGGGACACTTTCTCTTCT+TGG	0.187927	2.2:-62305197	None:intergenic
AGTGTTTAATCTTCTAAATT+TGG	0.209528	2.2:-62305419	None:intergenic
AATGGAGCGTGACATATTTA+TGG	0.230451	2.2:+62305169	MS.gene02388:CDS
GTTCTTGTTGTTTCTGTTGC+TGG	0.250824	2.2:-62304827	None:intergenic
CTATTTGGTTTAATTTGTTT+TGG	0.252179	2.2:-62304716	None:intergenic
GGCGAGATAGTGACACTATT+TGG	0.253327	2.2:-62304731	None:intergenic
TAGTCCTCTACGGATGCTTA+TGG	0.261788	2.2:+62305289	MS.gene02388:CDS
GGCATGTGAAATGTTTATTA+TGG	0.268895	2.2:+62305013	MS.gene02388:CDS
GTTGCTGGTGAGAGAATTCT+TGG	0.298214	2.2:-62304812	None:intergenic
ACCTATGATTTCAAAACTAA+TGG	0.307299	2.2:+62304903	MS.gene02388:CDS
AAATTTGGCAAATCAGTAGA+AGG	0.346833	2.2:-62305404	None:intergenic
GGTGGCTGCCCGTTGTAATT+AGG	0.360956	2.2:-62305332	None:intergenic
TAATAAGGAACATTCCCTAT+TGG	0.364276	2.2:-62305215	None:intergenic
GCCGGCATAGGCACATAATA+AGG	0.398907	2.2:-62305230	None:intergenic
TGCTTTGGGGTTGGAAACAT+TGG	0.399095	2.2:-62305371	None:intergenic
AATAAGGAACATTCCCTATT+GGG	0.409436	2.2:-62305214	None:intergenic
AGAAGAGAAAGTGTCCCAAT+AGG	0.412571	2.2:+62305200	MS.gene02388:CDS
ATGGAGCGTGACATATTTAT+GGG	0.438860	2.2:+62305170	MS.gene02388:CDS
AATAAACATTTCACATGCCT+TGG	0.439261	2.2:-62305009	None:intergenic
GGAAACATTGGATTAGCAAA+AGG	0.443443	2.2:-62305359	None:intergenic
GAAGAGAAAGTGTCCCAATA+GGG	0.454022	2.2:+62305201	MS.gene02388:CDS
CATGCCTTGGCAAACAGAAT+AGG	0.460292	2.2:-62304996	None:intergenic
AGTCCTCTACGGATGCTTAT+GGG	0.472646	2.2:+62305290	MS.gene02388:CDS
TCTACTCATGTAGGATGAAA+AGG	0.482954	2.2:-62304781	None:intergenic
TCCTTATTATGTGCCTATGC+CGG	0.483112	2.2:+62305229	MS.gene02388:CDS
CCTAGTTGATATTGTCCCTA+GGG	0.487741	2.2:+62305139	MS.gene02388:CDS
GAAACATTGGATTAGCAAAA+GGG	0.492038	2.2:-62305358	None:intergenic
TCAACCAACCTAATTACAAC+GGG	0.493047	2.2:+62305324	MS.gene02388:CDS
TGGAGCTAGCAACAAGGTCA+TGG	0.501489	2.2:+62305033	MS.gene02388:CDS
CCCTAGGGACAATATCAACT+AGG	0.511793	2.2:-62305139	None:intergenic
AAGATCATGAAAGCAGAAGA+AGG	0.528329	2.2:+62304948	MS.gene02388:CDS
TACTGAGGTGGCATATGAGC+CGG	0.534480	2.2:-62305248	None:intergenic
GCTGCCCGTTGTAATTAGGT+TGG	0.536604	2.2:-62305328	None:intergenic
CTAATGGCTTACCTTTAACA+AGG	0.540251	2.2:+62304919	MS.gene02388:CDS
CCGACACAAACACTAGCAAA+TGG	0.540457	2.2:+62304687	MS.gene02388:CDS
ATGCCACCTCAGTATGATGC+AGG	0.555708	2.2:+62305257	MS.gene02388:CDS
TCCTAGTTGATATTGTCCCT+AGG	0.558927	2.2:+62305138	MS.gene02388:CDS
GTCCATCATATAGTCCTCTA+CGG	0.567880	2.2:+62305279	MS.gene02388:CDS
GCATCAACAGTGTGAAACAA+AGG	0.568896	2.2:-62304753	None:intergenic
CATCAACAGTGTGAAACAAA+GGG	0.573192	2.2:-62304752	None:intergenic
GGCACCTATTCTGTTTGCCA+AGG	0.573214	2.2:+62304992	MS.gene02388:CDS
GGTGGCATATGAGCCGGCAT+AGG	0.576392	2.2:-62305242	None:intergenic
CCATTTGCTAGTGTTTGTGT+CGG	0.577743	2.2:-62304687	None:intergenic
GAGTTGAGCTCTACTCATGT+AGG	0.580476	2.2:-62304790	None:intergenic
GGAGCTAGCAACAAGGTCAT+GGG	0.582315	2.2:+62305034	MS.gene02388:CDS
ATCCGTAGAGGACTATATGA+TGG	0.585397	2.2:-62305281	None:intergenic
CTCAACCAACCTAATTACAA+CGG	0.612684	2.2:+62305323	MS.gene02388:CDS
AATTTGGCAAATCAGTAGAA+GGG	0.628469	2.2:-62305403	None:intergenic
AAAAGACACAAGAGGAACGA+AGG	0.630879	2.2:+62304655	MS.gene02388:CDS
CATGGATCAAAAGACACAAG+AGG	0.636214	2.2:+62304647	None:intergenic
AAGGTCATGGGCTAATACAG+AGG	0.637560	2.2:+62305046	MS.gene02388:CDS
ATTGGATTAGCAAAAGGGTG+TGG	0.642492	2.2:-62305353	None:intergenic
GGATTAGCAAAAGGGTGTGG+TGG	0.678099	2.2:-62305350	None:intergenic
AGTGAGTATGATATCAGCTG+AGG	0.690629	2.2:+62304971	MS.gene02388:CDS
TTATTATGGAGCTAGCAACA+AGG	0.697305	2.2:+62305027	MS.gene02388:CDS
GGACCTGCATCATACTGAGG+TGG	0.712395	2.2:-62305260	None:intergenic
CTTCCCATAAGCATCCGTAG+AGG	0.717998	2.2:-62305293	None:intergenic
GATGGACCTGCATCATACTG+AGG	0.765521	2.2:-62305263	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTATTTGGTTTAATTTGTTT+TGG	-	chr2.2:62304719-62304738	None:intergenic	20.0%
!	ACCTATGATTTCAAAACTAA+TGG	+	chr2.2:62304903-62304922	MS.gene02388:CDS	25.0%
!	ATCTTCTTAATCCTTGTTAA+AGG	-	chr2.2:62304933-62304952	None:intergenic	25.0%
	AAATTTGGCAAATCAGTAGA+AGG	-	chr2.2:62305407-62305426	None:intergenic	30.0%
	AATAAACATTTCACATGCCT+TGG	-	chr2.2:62305012-62305031	None:intergenic	30.0%
	AATAAGGAACATTCCCTATT+GGG	-	chr2.2:62305217-62305236	None:intergenic	30.0%
	AATTTGGCAAATCAGTAGAA+GGG	-	chr2.2:62305406-62305425	None:intergenic	30.0%
	GGCATGTGAAATGTTTATTA+TGG	+	chr2.2:62305013-62305032	MS.gene02388:CDS	30.0%
	TAATAAGGAACATTCCCTAT+TGG	-	chr2.2:62305218-62305237	None:intergenic	30.0%
!	GAAAAAACTTGGCATCTTTT+CGG	+	chr2.2:62304854-62304873	MS.gene02388:CDS	30.0%
!!	GAAACATTGGATTAGCAAAA+GGG	-	chr2.2:62305361-62305380	None:intergenic	30.0%
!!!	GCCATTAGTTTTGAAATCAT+AGG	-	chr2.2:62304907-62304926	None:intergenic	30.0%
	AAGATCATGAAAGCAGAAGA+AGG	+	chr2.2:62304948-62304967	MS.gene02388:CDS	35.0%
	AATGGAGCGTGACATATTTA+TGG	+	chr2.2:62305169-62305188	MS.gene02388:CDS	35.0%
	ATGGAGCGTGACATATTTAT+GGG	+	chr2.2:62305170-62305189	MS.gene02388:CDS	35.0%
	CAAGAACTGCAGAAAAAACT+TGG	+	chr2.2:62304843-62304862	MS.gene02388:CDS	35.0%
	CATCAACAGTGTGAAACAAA+GGG	-	chr2.2:62304755-62304774	None:intergenic	35.0%
	CTAATGGCTTACCTTTAACA+AGG	+	chr2.2:62304919-62304938	MS.gene02388:CDS	35.0%
	CTCAACCAACCTAATTACAA+CGG	+	chr2.2:62305323-62305342	MS.gene02388:CDS	35.0%
	TCAACCAACCTAATTACAAC+GGG	+	chr2.2:62305324-62305343	MS.gene02388:CDS	35.0%
	TCTACTCATGTAGGATGAAA+AGG	-	chr2.2:62304784-62304803	None:intergenic	35.0%
	TTATTATGGAGCTAGCAACA+AGG	+	chr2.2:62305027-62305046	MS.gene02388:CDS	35.0%
!!	AAGGGTCATTTTCTTGCTTT+GGG	-	chr2.2:62305388-62305407	None:intergenic	35.0%
!!	GGAAACATTGGATTAGCAAA+AGG	-	chr2.2:62305362-62305381	None:intergenic	35.0%
	AAAAGACACAAGAGGAACGA+AGG	+	chr2.2:62304655-62304674	MS.gene02388:CDS	40.0%
	AGTGAGTATGATATCAGCTG+AGG	+	chr2.2:62304971-62304990	MS.gene02388:CDS	40.0%
	ATCCGTAGAGGACTATATGA+TGG	-	chr2.2:62305284-62305303	None:intergenic	40.0%
	ATTGGGACACTTTCTCTTCT+TGG	-	chr2.2:62305200-62305219	None:intergenic	40.0%
	GCATCAACAGTGTGAAACAA+AGG	-	chr2.2:62304756-62304775	None:intergenic	40.0%
	GTCCATCATATAGTCCTCTA+CGG	+	chr2.2:62305279-62305298	MS.gene02388:CDS	40.0%
	TCCTTATTATGTGCCTATGC+CGG	+	chr2.2:62305229-62305248	MS.gene02388:CDS	40.0%
	TGTCCCTAGGGAAAAAACAA+TGG	+	chr2.2:62305151-62305170	MS.gene02388:CDS	40.0%
!	CCATTTGCTAGTGTTTGTGT+CGG	-	chr2.2:62304690-62304709	None:intergenic	40.0%
!	CCTAGTTGATATTGTCCCTA+GGG	+	chr2.2:62305139-62305158	MS.gene02388:CDS	40.0%
!	TCCTAGTTGATATTGTCCCT+AGG	+	chr2.2:62305138-62305157	MS.gene02388:CDS	40.0%
!!	AGAAGAGAAAGTGTCCCAAT+AGG	+	chr2.2:62305200-62305219	MS.gene02388:CDS	40.0%
!!	AGGGTCATTTTCTTGCTTTG+GGG	-	chr2.2:62305387-62305406	None:intergenic	40.0%
!!	ATTGGATTAGCAAAAGGGTG+TGG	-	chr2.2:62305356-62305375	None:intergenic	40.0%
!!	GAAGAGAAAGTGTCCCAATA+GGG	+	chr2.2:62305201-62305220	MS.gene02388:CDS	40.0%
!!	GAAGGGTCATTTTCTTGCTT+TGG	-	chr2.2:62305389-62305408	None:intergenic	40.0%
!!	GTTCTTGTTGTTTCTGTTGC+TGG	-	chr2.2:62304830-62304849	None:intergenic	40.0%
!!!	GCTCCATTGTTTTTTCCCTA+GGG	-	chr2.2:62305157-62305176	None:intergenic	40.0%
!!!	TCATTTTCTTGCTTTGGGGT+TGG	-	chr2.2:62305383-62305402	None:intergenic	40.0%
	AGTCCTCTACGGATGCTTAT+GGG	+	chr2.2:62305290-62305309	MS.gene02388:CDS	45.0%
	CATGCCTTGGCAAACAGAAT+AGG	-	chr2.2:62304999-62305018	None:intergenic	45.0%
	CCCTAGGGACAATATCAACT+AGG	-	chr2.2:62305142-62305161	None:intergenic	45.0%
	CCGACACAAACACTAGCAAA+TGG	+	chr2.2:62304687-62304706	MS.gene02388:CDS	45.0%
	GAGTTGAGCTCTACTCATGT+AGG	-	chr2.2:62304793-62304812	None:intergenic	45.0%
	GGCGAGATAGTGACACTATT+TGG	-	chr2.2:62304734-62304753	None:intergenic	45.0%
	GTTGCTGGTGAGAGAATTCT+TGG	-	chr2.2:62304815-62304834	None:intergenic	45.0%
	TAGTCCTCTACGGATGCTTA+TGG	+	chr2.2:62305289-62305308	MS.gene02388:CDS	45.0%
!	AAGGTCATGGGCTAATACAG+AGG	+	chr2.2:62305046-62305065	MS.gene02388:CDS	45.0%
!!	TGCTTTGGGGTTGGAAACAT+TGG	-	chr2.2:62305374-62305393	None:intergenic	45.0%
!!!	CGCTCCATTGTTTTTTCCCT+AGG	-	chr2.2:62305158-62305177	None:intergenic	45.0%
	ATGCCACCTCAGTATGATGC+AGG	+	chr2.2:62305257-62305276	MS.gene02388:CDS	50.0%
	CTTCCCATAAGCATCCGTAG+AGG	-	chr2.2:62305296-62305315	None:intergenic	50.0%
	GATGGACCTGCATCATACTG+AGG	-	chr2.2:62305266-62305285	None:intergenic	50.0%
	GCCGGCATAGGCACATAATA+AGG	-	chr2.2:62305233-62305252	None:intergenic	50.0%
	GCTGCCCGTTGTAATTAGGT+TGG	-	chr2.2:62305331-62305350	None:intergenic	50.0%
	GGAGCTAGCAACAAGGTCAT+GGG	+	chr2.2:62305034-62305053	MS.gene02388:CDS	50.0%
	GGCACCTATTCTGTTTGCCA+AGG	+	chr2.2:62304992-62305011	MS.gene02388:CDS	50.0%
	TACTGAGGTGGCATATGAGC+CGG	-	chr2.2:62305251-62305270	None:intergenic	50.0%
	TGGAGCTAGCAACAAGGTCA+TGG	+	chr2.2:62305033-62305052	MS.gene02388:CDS	50.0%
!	GGATTAGCAAAAGGGTGTGG+TGG	-	chr2.2:62305353-62305372	None:intergenic	50.0%
	GGACCTGCATCATACTGAGG+TGG	-	chr2.2:62305263-62305282	None:intergenic	55.0%
	GGTGGCTGCCCGTTGTAATT+AGG	-	chr2.2:62305335-62305354	None:intergenic	55.0%
	GGTGGCATATGAGCCGGCAT+AGG	-	chr2.2:62305245-62305264	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	62304648	62305436	62304648	ID=MS.gene02388
chr2.2	mRNA	62304648	62305436	62304648	ID=MS.gene02388.t1;Parent=MS.gene02388
chr2.2	exon	62304648	62305436	62304648	ID=MS.gene02388.t1.exon1;Parent=MS.gene02388.t1
chr2.2	CDS	62304648	62305436	62304648	ID=cds.MS.gene02388.t1;Parent=MS.gene02388.t1

Gene Sequence

>MS.gene02388

ATGGATCAAAAGACACAAGAGGAACGAAGGAATGATACACCGACACAAACACTAGCAAATGGCGAGAACCAAAACAAATTAAACCAAATAGTGTCACTATCTCGCCCTTTGTTTCACACTGTTGATGCTTCTGCCTTTTCATCCTACATGAGTAGAGCTCAACTCCAAGAATTCTCTCACCAGCAACAGAAACAACAAGAACTGCAGAAAAAACTTGGCATCTTTTCGGCAAAACAAAATGATGAAATTGTGAAGACCTATGATTTCAAAACTAATGGCTTACCTTTAACAAGGATTAAGAAGATCATGAAAGCAGAAGAAGGAGTGAGTATGATATCAGCTGAGGCACCTATTCTGTTTGCCAAGGCATGTGAAATGTTTATTATGGAGCTAGCAACAAGGTCATGGGCTAATACAGAGGTGAACAAAAGAAAGACACTTCAAAAGAGTGACATTGCATCTGCTGTCTCAAGTAATGAAGTGTTTGATTTCCTAGTTGATATTGTCCCTAGGGAAAAAACAATGGAGCGTGACATATTTATGGGTATACCAAGAAGAGAAAGTGTCCCAATAGGGAATGTTCCTTATTATGTGCCTATGCCGGCTCATATGCCACCTCAGTATGATGCAGGTCCATCATATAGTCCTCTACGGATGCTTATGGGAAGATATCTTCTCAACCAACCTAATTACAACGGGCAGCCACCACACCCTTTTGCTAATCCAATGTTTCCAACCCCAAAGCAAGAAAATGACCCTTCTACTGATTTGCCAAATTTAGAAGATTAA

Protein sequence

>MS.gene02388.t1

MDQKTQEERRNDTPTQTLANGENQNKLNQIVSLSRPLFHTVDASAFSSYMSRAQLQEFSHQQQKQQELQKKLGIFSAKQNDEIVKTYDFKTNGLPLTRIKKIMKAEEGVSMISAEAPILFAKACEMFIMELATRSWANTEVNKRKTLQKSDIASAVSSNEVFDFLVDIVPREKTMERDIFMGIPRRESVPIGNVPYYVPMPAHMPPQYDAGPSYSPLRMLMGRYLLNQPNYNGQPPHPFANPMFPTPKQENDPSTDLPNLED