AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023885

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023885.t1 MTR_7g077410 98.480 658 10 0 1 658 45 702 0.0 1356
MS.gene023885.t1 MTR_5g026210 51.307 612 274 7 48 656 60 650 0.0 636
MS.gene023885.t1 MTR_5g026210 53.004 466 196 6 48 510 60 505 1.25e-173 505
MS.gene023885.t1 MTR_5g026210 52.153 418 179 5 48 462 60 459 2.23e-149 441
MS.gene023885.t1 MTR_4g045667 48.630 438 215 4 220 651 12 445 3.55e-148 439
MS.gene023885.t1 MTR_2g089030 47.473 455 227 6 206 654 73 521 7.91e-142 426
MS.gene023885.t1 MTR_5g013640 46.256 454 224 7 199 648 68 505 4.40e-126 384
MS.gene023885.t1 MTR_5g013620 46.991 432 212 6 221 648 89 507 1.01e-123 378
MS.gene023885.t1 MTR_4g099110 46.292 445 228 7 208 648 65 502 1.09e-122 375
MS.gene023885.t1 MTR_8g099775 37.444 446 263 9 211 648 72 509 1.81e-88 286
MS.gene023885.t1 MTR_2g020240 36.866 434 255 8 221 649 83 502 7.34e-87 282
MS.gene023885.t1 MTR_6g477780 43.353 346 183 8 311 648 8 348 8.12e-85 271
MS.gene023885.t1 MTR_2g020240 36.939 379 221 7 275 649 3 367 3.83e-76 249
MS.gene023885.t1 MTR_6g477770 50.000 92 46 0 221 312 106 197 3.17e-25 103
MS.gene023885.t1 MTR_1g021990 47.253 91 45 1 37 127 13 100 3.18e-15 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023885.t1 AT2G45880 67.287 645 202 4 14 655 44 682 0.0 917
MS.gene023885.t1 AT4G00490 69.762 463 136 1 194 656 72 530 0.0 711
MS.gene023885.t1 AT5G45300 51.570 605 275 7 48 651 96 683 0.0 608
MS.gene023885.t1 AT5G45300 51.570 605 275 7 48 651 110 697 0.0 608
MS.gene023885.t1 AT5G45300 51.570 605 273 7 48 651 96 681 0.0 608
MS.gene023885.t1 AT2G32290 48.085 470 229 6 192 650 43 508 1.20e-142 428
MS.gene023885.t1 AT4G15210 46.697 439 223 6 220 651 13 447 2.66e-140 419
MS.gene023885.t1 AT4G17090 48.148 432 213 6 221 648 86 510 1.11e-133 404
MS.gene023885.t1 AT4G15210 48.101 395 198 4 220 611 13 403 9.01e-132 394
MS.gene023885.t1 AT3G23920 45.767 437 224 7 221 648 106 538 5.12e-127 387
MS.gene023885.t1 AT4G15210 45.477 398 207 5 260 651 1 394 1.31e-123 374
MS.gene023885.t1 AT4G15210 45.477 398 207 5 260 651 1 394 1.31e-123 374
MS.gene023885.t1 AT5G55700 38.479 447 251 9 221 651 92 530 6.99e-96 305
MS.gene023885.t1 AT5G18670 34.803 431 262 8 221 648 87 501 1.02e-68 234
MS.gene023885.t1 AT5G55700 38.304 342 191 8 322 651 34 367 1.11e-68 229

Find 199 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACGAATGTGGAGGCAATTT+TGG 0.132573 7.1:+26127290 MS.gene023885:CDS
AAGAAAGAGGGCACACTATT+TGG 0.160450 7.1:+26128605 MS.gene023885:CDS
CCTTCCATCTGGATCATTAA+TGG 0.228989 7.1:-26131442 None:intergenic
CCCCATTGGATTGCGGAAAT+TGG 0.229009 7.1:+26127335 MS.gene023885:CDS
TCCCTCAAGGGAGTTGCTTC+TGG 0.230220 7.1:+26125714 MS.gene023885:CDS
TACAGATATCAGGTATTTAT+TGG 0.240649 7.1:+26130924 MS.gene023885:intron
GCAATTATAATCTTAGAGTT+AGG 0.256212 7.1:+26124964 MS.gene023885:CDS
ATGCTCCTCAAGAGTATAAT+TGG 0.266684 7.1:+26127104 MS.gene023885:CDS
TATCTCCAACCATGCTTTAC+AGG 0.289028 7.1:-26127967 None:intergenic
AAAATCACGTTCTGGTAATC+TGG 0.293408 7.1:-26126698 None:intergenic
ATTCCCCAGGAAAGACATTC+AGG 0.294071 7.1:-26127407 None:intergenic
TTGATTTCATGAGAAGTTTC+CGG 0.295099 7.1:+26127854 MS.gene023885:CDS
AGTTAGCTATAACAAGGTTT+TGG 0.306669 7.1:+26131407 MS.gene023885:CDS
AGAAGCTGTTCTTGATCTTC+AGG 0.313620 7.1:+26131954 MS.gene023885:CDS
CGTTCAAACTCTATGAAGTT+TGG 0.314971 7.1:-26131516 None:intergenic
ATTTATTATATACAGATATC+AGG 0.333139 7.1:+26130914 MS.gene023885:intron
GGTGATTATGATGGCTTCTA+TGG 0.335535 7.1:+26128697 MS.gene023885:CDS
CTTCCATCTGGATCATTAAT+GGG 0.337375 7.1:-26131441 None:intergenic
GACAATCCTCCCTTGATTGA+TGG 0.338381 7.1:+26125867 MS.gene023885:CDS
TTGTAGGTACCCACATTATC+TGG 0.340452 7.1:-26128637 None:intergenic
TGTTATAGCTAACTCTGTTT+AGG 0.347312 7.1:-26131398 None:intergenic
TCCTGGTATTGGTGAGTTCC+AGG 0.351906 7.1:+26127990 MS.gene023885:CDS
GGATCTTGGCAGGGCTGCGC+AGG 0.352776 7.1:+26124937 MS.gene023885:CDS
TGAGCTTAAGCTTAAGTTGC+AGG 0.359918 7.1:+26127159 MS.gene023885:CDS
AAGGGAGTTGCTTCTGGCTA+TGG 0.363095 7.1:+26125720 MS.gene023885:CDS
GGTGGGAGTGGGATGGGAAC+TGG 0.366608 7.1:+26124771 MS.gene023885:CDS
ATCTGGATCATTAATGGGCT+TGG 0.366707 7.1:-26131436 None:intergenic
ATCACATAAACAGGAACATA+TGG 0.371428 7.1:-26126730 None:intergenic
AGAAAGAGGGCACACTATTT+GGG 0.383259 7.1:+26128606 MS.gene023885:CDS
TTCCCGATCTCAAACATCTA+TGG 0.384478 7.1:+26125821 MS.gene023885:CDS
AAATACCTGCTTCTCTCTAA+TGG 0.392328 7.1:-26125901 None:intergenic
TGACTGCTGGTGGGGAATAG+TGG 0.395519 7.1:+26127075 MS.gene023885:CDS
ACAACCCTGAATGTCTTTCC+TGG 0.395545 7.1:+26127402 MS.gene023885:CDS
ACCAATTTCCGCAATCCAAT+GGG 0.401881 7.1:-26127336 None:intergenic
CTGATCCCGAGCGATTAGTT+TGG 0.407402 7.1:+26131122 MS.gene023885:CDS
ACCGGTAGCATCACATAAAC+AGG 0.415437 7.1:-26126739 None:intergenic
GTTCTCTCTTTCTCTTCTAC+CGG 0.419911 7.1:-26124876 None:intergenic
GACATGCATTATAGTCTAAA+GGG 0.422702 7.1:-26125746 None:intergenic
TCGGGGATGGTAGAGATTGA+TGG 0.429329 7.1:+26124750 None:intergenic
CTTTAGTTGATCATGGAAAC+CGG 0.430369 7.1:+26128749 MS.gene023885:CDS
GATGAAAAGTGCCTTCCATC+TGG 0.431328 7.1:-26131453 None:intergenic
GGTATGAAAACATCTTTCAT+TGG 0.431483 7.1:-26125768 None:intergenic
AGCAAATGTTGATGGTGTTA+TGG 0.432333 7.1:+26127051 MS.gene023885:CDS
TCTCAGGCTTTAGTTGATCA+TGG 0.433442 7.1:+26128742 MS.gene023885:CDS
GGCTATCACTGCAAGGATCT+TGG 0.439690 7.1:+26124923 MS.gene023885:CDS
CAAGAGCAAGCAGGCACTCC+TGG 0.439805 7.1:+26124831 MS.gene023885:CDS
TCAGAAGTGGACTGAGCCTC+AGG 0.439950 7.1:-26131485 None:intergenic
ATTATGATGGCTTCTATGGC+AGG 0.443004 7.1:+26128701 MS.gene023885:CDS
AATACTCATTAAACTCTATC+CGG 0.443205 7.1:-26127873 None:intergenic
AGTGGGATGGGAACTGGAGC+CGG 0.444427 7.1:+26124777 MS.gene023885:CDS
TTGGCAGGGCTGCGCAGGCA+TGG 0.447154 7.1:+26124942 MS.gene023885:CDS
GAAGTTGAGGACAGATCATA+TGG 0.448226 7.1:-26125801 None:intergenic
ATTGATGGTGGGAGTGGGAT+GGG 0.452488 7.1:+26124765 MS.gene023885:CDS
AATTCTCAGCCACACGAAAC+GGG 0.456914 7.1:+26128658 MS.gene023885:CDS
CTCCCAGCAAAATCACGTTC+TGG 0.459187 7.1:-26126706 None:intergenic
CAATTCTCAGCCACACGAAA+CGG 0.459319 7.1:+26128657 MS.gene023885:CDS
TTTGTATTCCATTACCCCAT+TGG 0.460459 7.1:+26127321 MS.gene023885:CDS
GGACATGCATTATAGTCTAA+AGG 0.460742 7.1:-26125747 None:intergenic
CAATTATAATCTTAGAGTTA+GGG 0.460826 7.1:+26124965 MS.gene023885:CDS
GAAGGAAAAGTAGTTCCATC+TGG 0.463042 7.1:-26125044 None:intergenic
TTGAATGCCGGGTGGGATGT+TGG 0.467187 7.1:+26131342 MS.gene023885:CDS
CACCATAGATGTTTGAGATC+GGG 0.467798 7.1:-26125823 None:intergenic
ATCTATTGAACCATCAATCA+AGG 0.468065 7.1:-26125877 None:intergenic
ATCACTGCAAGGATCTTGGC+AGG 0.470853 7.1:+26124927 MS.gene023885:CDS
TTCGTGTGGCTGAGAATTGT+AGG 0.472590 7.1:-26128653 None:intergenic
GAACTGGAGCCGGTGACAAC+AGG 0.472912 7.1:+26124787 MS.gene023885:CDS
TTCATCTCCATGATTGAAGT+TGG 0.474972 7.1:+26127907 MS.gene023885:CDS
GCCAGAAGCAACTCCCTTGA+GGG 0.477029 7.1:-26125715 None:intergenic
AGATACCATTAGAGAGAAGC+AGG 0.479796 7.1:+26125896 MS.gene023885:CDS
TCATACCTTTGCAGCAATGC+AGG 0.480344 7.1:-26128806 None:intergenic
GTATTGCAGGTGTTGAATGC+CGG 0.481649 7.1:+26131330 MS.gene023885:intron
AGGGAGTTGCTTCTGGCTAT+GGG 0.481665 7.1:+26125721 MS.gene023885:CDS
AGAAAAGCAGCAGAAGAAAG+AGG 0.481803 7.1:+26128592 MS.gene023885:CDS
AAATCACGTTCTGGTAATCT+GGG 0.482509 7.1:-26126697 None:intergenic
TCCGCAATCCAATGGGGTAA+TGG 0.483794 7.1:-26127329 None:intergenic
CTCTAGCAAGGGAAGCTGGA+TGG 0.485605 7.1:+26125012 MS.gene023885:CDS
CAAAGCTAAGAGAGAGGCGC+CGG 0.488485 7.1:+26124898 MS.gene023885:CDS
CAACCCTGAATGTCTTTCCT+GGG 0.489155 7.1:+26127403 MS.gene023885:CDS
GCTGCTCTAGCAAGGGAAGC+TGG 0.494031 7.1:+26125008 MS.gene023885:CDS
GTAGAGATTGATGGTGGGAG+TGG 0.497271 7.1:+26124759 MS.gene023885:CDS
GTGTTATGGTTGACTGCTGG+TGG 0.497598 7.1:+26127065 MS.gene023885:CDS
AGCTAAGAGAGAGGCGCCGG+AGG 0.498212 7.1:+26124901 MS.gene023885:CDS
AATCGCTCGGGATCAGCAAA+TGG 0.498861 7.1:-26131115 None:intergenic
TAAATGTGAGCTTGTTGATC+CGG 0.500589 7.1:+26126994 MS.gene023885:CDS
TGGAAACCGGGTACTTTCAA+TGG 0.501041 7.1:+26128762 MS.gene023885:CDS
TCGGGATCAGCAAATGGCTC+TGG 0.502776 7.1:-26131109 None:intergenic
ACCTAGTCCAACTTCAATCA+TGG 0.503665 7.1:-26127914 None:intergenic
AAGCATGGTTGGAGATATCC+TGG 0.503866 7.1:+26127973 MS.gene023885:CDS
CCTCCATCTGTTTCCCTCAA+GGG 0.504458 7.1:+26125702 MS.gene023885:CDS
GATTGATGGTGGGAGTGGGA+TGG 0.506994 7.1:+26124764 MS.gene023885:CDS
TACCAATTTCCGCAATCCAA+TGG 0.510010 7.1:-26127337 None:intergenic
ATGGTGTTATGGTTGACTGC+TGG 0.511687 7.1:+26127062 MS.gene023885:CDS
CAAGCAGGCACTCCTGGTGG+AGG 0.514225 7.1:+26124837 MS.gene023885:CDS
ATTGCATGGATTATAATATC+CGG 0.517718 7.1:-26130984 None:intergenic
TTGAAATCAGCAAATGTTGA+TGG 0.518512 7.1:+26127043 MS.gene023885:CDS
GGCTAGAGGGCCAGATAATG+TGG 0.519736 7.1:+26128627 MS.gene023885:CDS
GTTATGGTTGACTGCTGGTG+GGG 0.522259 7.1:+26127067 MS.gene023885:CDS
GCCTCCATCTGTTTCCCTCA+AGG 0.524523 7.1:+26125701 MS.gene023885:CDS
CTTGTCCTGTAAAGCATGGT+TGG 0.525812 7.1:+26127962 MS.gene023885:CDS
GAGGCTCAGTCCACTTCTGA+TGG 0.525869 7.1:+26131488 MS.gene023885:CDS
TTACAAGAGACTCTTTCAAA+TGG 0.526474 7.1:+26127132 MS.gene023885:CDS
GGCACACTATTTGGGCTAGA+GGG 0.530326 7.1:+26128614 MS.gene023885:CDS
GGTTGGAGATATCCTGGTAT+TGG 0.531486 7.1:+26127979 MS.gene023885:CDS
TGTTATGGTTGACTGCTGGT+GGG 0.531507 7.1:+26127066 MS.gene023885:CDS
TCTAGCAAGGGAAGCTGGAT+GGG 0.532361 7.1:+26125013 MS.gene023885:CDS
GGGATCCTGCATTGCTGCAA+AGG 0.534136 7.1:+26128801 MS.gene023885:CDS
AGTTTGAACGATTTGTCAAG+AGG 0.534218 7.1:+26131529 MS.gene023885:CDS
CCACCATAGATGTTTGAGAT+CGG 0.535969 7.1:-26125824 None:intergenic
GACTTGCCAAACTAATCGCT+CGG 0.538712 7.1:-26131128 None:intergenic
CCATGCTTTACAGGACAAGA+TGG 0.541344 7.1:-26127958 None:intergenic
ACAGCAATGTTAAAGAGAAA+CGG 0.542903 7.1:+26131028 MS.gene023885:CDS
TCCATTACCCCATTGGATTG+CGG 0.544817 7.1:+26127328 MS.gene023885:CDS
ATCTCAAACATCTATGGTGG+GGG 0.545777 7.1:+26125827 MS.gene023885:CDS
ACGTTCCTTATCTATTCCCC+AGG 0.549619 7.1:-26127420 None:intergenic
GGATGGGTGGTTCTTCCAGA+TGG 0.550123 7.1:+26125029 MS.gene023885:CDS
TCTTTCCTGGGGAATAGATA+AGG 0.553960 7.1:+26127415 MS.gene023885:CDS
CCTCAAGAGTATAATTGGAA+TGG 0.554372 7.1:+26127109 MS.gene023885:CDS
ATGTCATTGCTGCTCTAGCA+AGG 0.555742 7.1:+26125000 MS.gene023885:CDS
ACAGATGGAGGCTGTTGAGA+AGG 0.557652 7.1:-26125690 None:intergenic
TGTTTGAGATCGGGAAGTTG+AGG 0.559441 7.1:-26125814 None:intergenic
CCATTCCAATTATACTCTTG+AGG 0.560764 7.1:-26127109 None:intergenic
GATCTTCGATTGCCTCCACC+AGG 0.561586 7.1:-26124849 None:intergenic
CATGCTGCTGAATTAGCTGC+CGG 0.562553 7.1:+26130965 MS.gene023885:CDS
TTTGGTCGTTCCATCAGAAG+TGG 0.562705 7.1:-26131498 None:intergenic
CGATCTCAAACATCTATGGT+GGG 0.562927 7.1:+26125825 MS.gene023885:CDS
TTCCCCAGGAAAGACATTCA+GGG 0.566249 7.1:-26127406 None:intergenic
TATTGCAGGTGTTGAATGCC+GGG 0.566994 7.1:+26131331 MS.gene023885:intron
CAAAAGAAACCCGTTTCGTG+TGG 0.568671 7.1:-26128667 None:intergenic
TCACATAAACAGGAACATAT+GGG 0.570006 7.1:-26126729 None:intergenic
AGCTGCTTTAGTATACCATC+CGG 0.570265 7.1:-26127013 None:intergenic
TCCTTGCAGTGATAGCCCTC+CGG 0.572338 7.1:-26124917 None:intergenic
ATTGGATTGCGGAAATTGGT+AGG 0.573552 7.1:+26127339 MS.gene023885:CDS
CCGATCTCAAACATCTATGG+TGG 0.574081 7.1:+26125824 MS.gene023885:CDS
GGGATGGTAGAGATTGATGG+TGG 0.574890 7.1:+26124753 None:intergenic
AGATATCAGGTATTTATTGG+TGG 0.576841 7.1:+26130927 MS.gene023885:intron
ACTTGCCAAACTAATCGCTC+GGG 0.578201 7.1:-26131127 None:intergenic
TTACAGGGACAGATGCCAGC+TGG 0.579767 7.1:+26125627 MS.gene023885:intron
TAGCTAACTCTGTTTAGGCA+AGG 0.580773 7.1:-26131393 None:intergenic
ACCTGGAACTCACCAATACC+AGG 0.580960 7.1:-26127991 None:intergenic
GCATAGCCATCACGATTGCA+TGG 0.581501 7.1:-26130998 None:intergenic
AAGCCCATTAATGATCCAGA+TGG 0.584215 7.1:+26131438 MS.gene023885:CDS
CTGCTTGCTCTTGAAACTCG+TGG 0.584946 7.1:-26124821 None:intergenic
TAGAGATTGATGGTGGGAGT+GGG 0.585198 7.1:+26124760 MS.gene023885:CDS
ACTGAGCCTCAGGTATGTAA+AGG 0.587637 7.1:-26131475 None:intergenic
GAGGCTGTTGAGAAGGCACA+TGG 0.587814 7.1:-26125683 None:intergenic
TTGCTGAAACCTGTTGTCAC+CGG 0.588256 7.1:-26124796 None:intergenic
TCCTGTTTATGTGATGCTAC+CGG 0.588479 7.1:+26126738 MS.gene023885:CDS
GAAAAGCAGCAGAAGAAAGA+GGG 0.589579 7.1:+26128593 MS.gene023885:CDS
CGATTTGTCAAGAGGATGCA+CGG 0.590836 7.1:+26131537 MS.gene023885:CDS
AGATCATATGGAGATGGAGA+TGG 0.591075 7.1:-26125789 None:intergenic
GGGCACACTATTTGGGCTAG+AGG 0.593100 7.1:+26128613 MS.gene023885:CDS
GAGGACAGATCATATGGAGA+TGG 0.593231 7.1:-26125795 None:intergenic
TTTCATCCTTTACATACCTG+AGG 0.594089 7.1:+26131469 MS.gene023885:CDS
GAGAGGTCGGACTGCTGTGG+AGG 0.594354 7.1:+26127448 MS.gene023885:CDS
ATCACGTTCTGGTAATCTGG+GGG 0.596266 7.1:-26126695 None:intergenic
ATGGTATACTAAAGCAGCTG+AGG 0.597687 7.1:+26127017 MS.gene023885:CDS
GCTAAGAGAGAGGCGCCGGA+GGG 0.598262 7.1:+26124902 MS.gene023885:CDS
CACGAGTTTCAAGAGCAAGC+AGG 0.600604 7.1:+26124822 MS.gene023885:CDS
AGCCAGAAGCAACTCCCTTG+AGG 0.601428 7.1:-26125716 None:intergenic
ACTGCAGAATTACCACCAGC+TGG 0.602253 7.1:-26125642 None:intergenic
GATCTCAAACATCTATGGTG+GGG 0.602792 7.1:+26125826 MS.gene023885:CDS
CCATCTTGTCCTGTAAAGCA+TGG 0.604293 7.1:+26127958 MS.gene023885:CDS
AGCAAGGGAAGCTGGATGGG+TGG 0.608234 7.1:+26125016 MS.gene023885:CDS
CATGCTTTACAGGACAAGAT+GGG 0.612017 7.1:-26127957 None:intergenic
GCCGGAGGGCTATCACTGCA+AGG 0.616217 7.1:+26124916 MS.gene023885:CDS
GAGCAAGCAGGCACTCCTGG+TGG 0.616975 7.1:+26124834 MS.gene023885:CDS
AGTGCATTCTCACTAACGAC+GGG 0.621609 7.1:-26131369 None:intergenic
TATAATCCATGCAATCGTGA+TGG 0.623009 7.1:+26130992 MS.gene023885:CDS
AGAGAACAAAGCTAAGAGAG+AGG 0.624197 7.1:+26124892 MS.gene023885:CDS
AGGCAATCGAAGATCTAGAC+CGG 0.625299 7.1:+26124857 MS.gene023885:CDS
TTTAGTTGATCATGGAAACC+GGG 0.629705 7.1:+26128750 MS.gene023885:CDS
CCATTAATGATCCAGATGGA+AGG 0.631548 7.1:+26131442 MS.gene023885:CDS
TCCATGATTGAAGTTGGACT+AGG 0.633760 7.1:+26127913 MS.gene023885:CDS
CTGCTGGTGGGGAATAGTGG+AGG 0.638557 7.1:+26127078 MS.gene023885:CDS
ACTCCCTTGAGGGAAACAGA+TGG 0.640870 7.1:-26125705 None:intergenic
TCTATTGAACCATCAATCAA+GGG 0.641869 7.1:-26125876 None:intergenic
AAACAGAGTTAGCTATAACA+AGG 0.642740 7.1:+26131401 MS.gene023885:CDS
GCAGGTGTTGAATGCCGGGT+GGG 0.648360 7.1:+26131335 MS.gene023885:intron
TGTCATTGCTGCTCTAGCAA+GGG 0.650040 7.1:+26125001 MS.gene023885:CDS
TGCAGGTGTTGAATGCCGGG+TGG 0.651385 7.1:+26131334 MS.gene023885:intron
AACCCTGAATGTCTTTCCTG+GGG 0.652109 7.1:+26127404 MS.gene023885:CDS
AATCACGTTCTGGTAATCTG+GGG 0.653035 7.1:-26126696 None:intergenic
TGTGAGCTTGTTGATCCGGA+TGG 0.654767 7.1:+26126998 MS.gene023885:CDS
TGGTATACTAAAGCAGCTGA+GGG 0.655208 7.1:+26127018 MS.gene023885:CDS
GGGTAGTCCAACATCCCACC+CGG 0.657727 7.1:-26131349 None:intergenic
GGATGGTAGAGATTGATGGT+GGG 0.658760 7.1:+26124754 None:intergenic
TCACTGCAAGGATCTTGGCA+GGG 0.658891 7.1:+26124928 MS.gene023885:CDS
CCCTTGAGGGAAACAGATGG+AGG 0.658899 7.1:-26125702 None:intergenic
TTTGAGAGGTCGGACTGCTG+TGG 0.661975 7.1:+26127445 MS.gene023885:CDS
GAATGTGTGCAAATCAACAC+AGG 0.666849 7.1:-26131068 None:intergenic
AAGTGCATTCTCACTAACGA+CGG 0.670203 7.1:-26131370 None:intergenic
CAGGGACAGATGCCAGCTGG+TGG 0.672850 7.1:+26125630 MS.gene023885:intron
GGGTATCTTAGCTCTCCACA+TGG 0.681100 7.1:-26127937 None:intergenic
ATTGAACCATCAATCAAGGG+AGG 0.685955 7.1:-26125873 None:intergenic
GCTAGAGGGCCAGATAATGT+GGG 0.688613 7.1:+26128628 MS.gene023885:CDS
TCACGTTCTGGTAATCTGGG+GGG 0.695800 7.1:-26126694 None:intergenic
GAAGTTGGACTAGGTCCATG+TGG 0.708297 7.1:+26127922 MS.gene023885:CDS
CCAATTTCCGCAATCCAATG+GGG 0.711257 7.1:-26127335 None:intergenic
AGGCTGTTGAGAAGGCACAT+GGG 0.748056 7.1:-26125682 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.1 gene 26124756 26131981 26124756 ID=MS.gene023885
chr7.1 mRNA 26124756 26131981 26124756 ID=MS.gene023885.t1;Parent=MS.gene023885
chr7.1 exon 26124756 26125076 26124756 ID=MS.gene023885.t1.exon1;Parent=MS.gene023885.t1
chr7.1 CDS 26124756 26125076 26124756 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26125633 26125917 26125633 ID=MS.gene023885.t1.exon2;Parent=MS.gene023885.t1
chr7.1 CDS 26125633 26125917 26125633 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26126682 26126759 26126682 ID=MS.gene023885.t1.exon3;Parent=MS.gene023885.t1
chr7.1 CDS 26126682 26126759 26126682 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26126977 26127180 26126977 ID=MS.gene023885.t1.exon4;Parent=MS.gene023885.t1
chr7.1 CDS 26126977 26127180 26126977 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26127275 26127469 26127275 ID=MS.gene023885.t1.exon5;Parent=MS.gene023885.t1
chr7.1 CDS 26127275 26127469 26127275 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26127847 26128011 26127847 ID=MS.gene023885.t1.exon6;Parent=MS.gene023885.t1
chr7.1 CDS 26127847 26128011 26127847 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26128562 26128822 26128562 ID=MS.gene023885.t1.exon7;Parent=MS.gene023885.t1
chr7.1 CDS 26128562 26128822 26128562 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26130929 26131147 26130929 ID=MS.gene023885.t1.exon8;Parent=MS.gene023885.t1
chr7.1 CDS 26130929 26131147 26130929 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26131339 26131558 26131339 ID=MS.gene023885.t1.exon9;Parent=MS.gene023885.t1
chr7.1 CDS 26131339 26131558 26131339 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
chr7.1 exon 26131953 26131981 26131953 ID=MS.gene023885.t1.exon10;Parent=MS.gene023885.t1
chr7.1 CDS 26131953 26131981 26131953 ID=cds.MS.gene023885.t1;Parent=MS.gene023885.t1
Gene Sequence

>MS.gene023885

ATGGTAGAGATTGATGGTGGGAGTGGGATGGGAACTGGAGCCGGTGACAACAGGTTTCAGCAACACCACGAGTTTCAAGAGCAAGCAGGCACTCCTGGTGGAGGCAATCGAAGATCTAGACCGGTAGAAGAGAAAGAGAGAACAAAGCTAAGAGAGAGGCGCCGGAGGGCTATCACTGCAAGGATCTTGGCAGGGCTGCGCAGGCATGGCAATTATAATCTTAGAGTTAGGGCTGACATTAATGATGTCATTGCTGCTCTAGCAAGGGAAGCTGGATGGGTGGTTCTTCCAGATGGAACTACTTTTCCTTCAAAATCTCAGGTAATTATTTGTCAAGACTTAAACTGCATTATTTGATTCCGAAACTAAATGTTAGTTAGATTTGTACGGGTTCAAGTCTTGGAAACAACTTCTTGTGTAAAAACAAGGTAGGGCTGTGTACAAAACACCAAATGGTGGGACCCCTTCCCAGACCCTGCGAATGCGGGAGCTTTAGTGCACCGGGCTGCCCTTTTTTAGTATGCATCTTCTGGCGTCATTTGCTGTTTCTCCATGAAAGGAGGGACTATGGCCTGATCATTTTTTTCGTCCAGTTGTTTATTCTTCGAAAAACCCTATTCAACATTTGTTTATGATAGCTCTATTGTTGAGAAAATACTTAATTTTTGATGTAGTGTGGACATTAATTATTTGTACGTTTCAACTTGTTATATAGTTTAGTTCTTGGCTTAGTCAAGTCAAACCTATTTGATATTTTAAATGTCTTATTACCACTTTGTATCTGCAAATCTTATTACTGCTGTTTATCTGCGAATTTCAGTTCTGATAAATCTCAGAAAAACTCCCACATTGGAACCTTTTCTTCTCAACCTTACAGGGACAGATGCCAGCTGGTGGTAATTCTGCAGTTGTTACTTCATCATCATCCCATGTGCCTTCTCAACAGCCTCCATCTGTTTCCCTCAAGGGAGTTGCTTCTGGCTATGGGAGCCCTTTAGACTATAATGCATGTCCAATGAAAGATGTTTTCATACCATCTCCATCTCCATATGATCTGTCCTCAACTTCCCGATCTCAAACATCTATGGTGGGGGATGGGGAAGCGCAAAGAGACAATCCTCCCTTGATTGATGGTTCAATAGATACCATTAGAGAGAAGCAGGTATTTAATGGAGCTGATTGGCCTCTTCTTGATCTCTTTTAGAAATAAATGTTTTTATGGTTTCTGTGGTGATATGGTGATGGAATGATCTAGCGGATACATTAGTATTTCTTTCCAAATAAATATCAAAATTTTACTCATTTAGTTTAGAAGCAGAACATGTTAAATTGACCTTTAATGAGTAAAATGTGTATCGAAAATATAGCATCTTACTTGCTTTGTGAATTTTATATGGACTGTCGTTGTGACCCTCTTGATTTCTTTTATATTTTCTTCGACTTGATCTGCCAAATTTGATAGACACATTTAAAAGGATAAACACATGATTAAGGTTCGTGAGGGGTACATCATACAAATTTAAGGAAAGTTTATGTTGTTTAAGCATAATGGATCCTGAGCTGGTGACGACCAAGGTGGACTCATTTGGGGAGGAGAATATAGGAGGGAAGGATAGATAGTGTATGGTAGGCATGAATTTAGAGGAAAAAGTTGAATGGCTTTAAGCTAGAGGAGGTTCTGTGCGTTTTCTTAACTCTAGATTTATCTTTTATTTTCTCTATTTTCTGTTCTCGTAAGGGCTAATCATCACAGTTTTTATCAGTAAAAATCAGTCCCTATAGTTAAGAAAATTTGAAATGATATCCCAAGATGAATGATTATCCAAAAAGGGATATTCTTCCGTTTTATTTTGCATATAATTTCAAAATATGGTGCCTTTAGAAGGTAAGTCTCTTGGCATTGTTTACATCTTTTTATCCTTACAGATTGCTGACATTCCCCCCAGATTACCAGAACGTGATTTTGCTGGGAGCCCATATGTTCCTGTTTATGTGATGCTACCGGTAAGCTATGATTTTACTCTTATTTTTCATTTTCTATTAAGTCCTGTTTCTTGGTTTCTTTAATTACTCGTAGTAAATAGTTATGCTTAATTAATATGTTAATATGTTGATGTTCTCAGTATATATTTTGGATTTTCTTTATATGATTATCTTGTTGGAAATCACCTATATGCGTCGATTAACATTATGTACCATATTCTATTTATATTGTTGACAGTTGGGGGTGATCAATATTAAATGTGAGCTTGTTGATCCGGATGGTATACTAAAGCAGCTGAGGGTATTGAAATCAGCAAATGTTGATGGTGTTATGGTTGACTGCTGGTGGGGAATAGTGGAGGCACATGCTCCTCAAGAGTATAATTGGAATGGTTACAAGAGACTCTTTCAAATGGTGCGTGAGCTTAAGCTTAAGTTGCAGGTGAGTTTTATACGCTACTTTAATCATCGTGGGTTGCTCTCTTGCTGATTCAGTCTGGCTTTTAATGTGAATTATCTCATATACATTTGGGTAGGTTGTCATGTCTTTTCACGAATGTGGAGGCAATTTTGGTGATGATGTTTGTATTCCATTACCCCATTGGATTGCGGAAATTGGTAGGAGCAATCCAGATATTTTTTTCACTGATAGAGAGGGAAGACACAACCCTGAATGTCTTTCCTGGGGAATAGATAAGGAACGTGTTTTGAGAGGTCGGACTGCTGTGGAGGTAATAACGACGTCATAATACGATTACTTATTGTTGTTTGCTTTTGTTGCTGCTATTGTTATTAACAACTATTGTTGTTGTTGTTGTTATTACAATTTACAATTGAATGCTGTCAGAAAGAAATGGATTTTTGCTTATGCTTAATGAAAAGCCATAGTGTAGACATCTGTAAACCGAGCTTGAATTAAATCATGATGGTGAAGGCTTTAATCCATCCTTTATACATATTATGGTGTTAATCTTAAATCACCATTCCTCTGAATGAATCTATTTGGATCATTTATCAAATGTTTTTCAAGGATCCGAGAGGAGACATGGTTTCCTCTGTTATACCAAGAAAATTAAGGCATGCAATTTGTGTGTTGTATTTTTTTTAGGTTTATTTTGATTTCATGAGAAGTTTCCGGATAGAGTTTAATGAGTATTTTGAGGATGGCTTCATCTCCATGATTGAAGTTGGACTAGGTCCATGTGGAGAGCTAAGATACCCATCTTGTCCTGTAAAGCATGGTTGGAGATATCCTGGTATTGGTGAGTTCCAGGTAATTACTTTCTTCAATATACGTTTTTTTTTTTTTGTTGCTTTCTCAAGTGTATTATAGCTGTTCTCTAAATCTGATAATTTCCAGATTCAATTTTTCATTGTGAATGCCTCCGTAGCTCAAATTTCTTTGTTTCTTGTAATTCTTTTAACCAAGAAAATCCTCATTCTGCTGAGCCTCAAAAATATATTTTTTTAAAAATACCTTGTACAGGGACATTTTCTTAAAAGGTAATTGTTTCGTTCTTTGTCCTCCTTAATACTTTCTTCTATCACATGCTTTCATTGGCATTTTGTTTAGGACTGGGTTCTTCATTCAACATCTATATATAGGGACAATTGTTTGTTTGTTGGTGTTAGATAGTGTATATGAGTGAGTTAAATGTTCATTGTTTGCTGCCGTGTATTGTCACAGACTATGGATCTATCTCTGTCTCTTATTCTTCACTTATAGTTACTGATGTGTTTTTTGCCTGTATGCATACATACACTTCAGAGGCTCAAATACCTTCTTAACCTATTTGCTAATCATCTTTCTCACCTTTGTGCAGTGCTATGATCAGTATATGTTGAAAAGTCTTAGAAAAGCAGCAGAAGAAAGAGGGCACACTATTTGGGCTAGAGGGCCAGATAATGTGGGTACCTACAATTCTCAGCCACACGAAACGGGTTTCTTTTGCGATGGGGGTGATTATGATGGCTTCTATGGCAGGTTTTTTCTCAACTGGTACTCTCAGGCTTTAGTTGATCATGGAAACCGGGTACTTTCAATGGCAAAATTAGCTTTTGAGGGATCCTGCATTGCTGCAAAGGTATGATAACAAAAAATTATAATGTGAATATACTTTTGTAATCTGTTAGAAAAAAAGCTTCTTGAATTATTTTCCGCATCATCATATGCTTAATGAGTGTTTGCTCATTCCACCTATTTGATTAGGGCTTTAGTAGTTGGTGTTTGTTTATGAATGATATCATTATAGAAAAGATGGTTGAATCTTGATTTAGGTGGTTTGGACATGTGGGGAGGAGACCTATAGATTCCGCAGTAAGGAGGGTAGATTAGATGGAGGATAGGCCAATTGCTAGAGGCAGAGGAAGACCAAGAAAAACTGTAGGCGAAACTATCAAGAAAGACCTAGAACTCAATTGTTTGGATAAAAATATAACATCGTGATAGAGTATTATGACGTCATTTGATTCATATAGCCTACCCCACCTAGTCAGTGGCGGAGCCAGACAAAAATGTTTGGGATGGCCGACATCAATATCATTGGTCATCCTCTTGTAAAATGAATAGATTTTTTGATTCTTCATCTTGACTTTTCTGTTTCTAGGAAACAAATTTATCAAAATGATGTAAGAATAGTGTTAAGACACTCATAATTAAACAAATCTACAATTGACAATGGTACTGCTAATGTACTTAAATTTCATGGTCTTACTCTTTTGTTAAAACCAACATCATTATGCTTCTCAATTACATACATATTTTCACCAATGAATTTAACAAAAAATGATAGTGTTACTGTTACATTTTACAATATTATATAACTAGGAGTTGTTATGTTAAAACAGTTTATGTTAAAAACTAAGTTATAAGTGAAATTACATAGAACTAAATTGCAATGTACGGTACAAACTCAACCCATTAGAAAGATTTGTCAGGATTGCATAAGGAAACAAACTCAGGAGTCAGGATAACAGATAATAAAGATACATTGCTTCTGCCTTCTACAAGACTACAACGAAACATTTTTCAAAATCTTTTGCACTAAAATTTTGAAGATTAAATTTCAAAATCTTCTAGCAATTTGAGGGCACTGAAAAGGAAAAAAAAACCTCGGTAATGGCTCAAATCAAAAATAAAAAATCCCCTTTTTGTTTTGATTAGGGTTATGGGAAAATTGAAATTGAAAACCAAAACCAAAATCAAATTCATCAACAAACAAAAAGAAGATGAAGAATCAGATTCAGAGATACACTTAGAATTGTAGGGTTTGTAGAGAGATTAAGACCAATGAAGAATAGACTCGGATTCTGGAGATTGATGCAGATAGAAGAGATGCCGGTGGATGAGTTCGCCGGAGGAGATGCCGGTGGGTGATGGCGGTGAGTCGTGAGTGATGTTGTGCAAGGGAGAAGAGAAAATACACTTCAGCTCTGGTTCTTTTATTTCCCTTTCCCGTTCTGGAGAAAAATGGCATTGAGAGTGGGGTAGTTGAGTGGCATGGCAGTAATATTAAATTAGCTTAGGGGTATTATAGTCTTTTCACAGAATAAATGGGTAGGCCATGGCCAGTCCCAGTCCACACGTGGCTCCGCCACTGCACCTAGTGGGATACGGCTTGGTTGTTCTTGTTGTTTGTTCTTGTAACGCCTTGCCAATTTTTCATACCATCAGAAGTTTCTAATTGATGGGGTCCTCCACTTGTGCTATTAATTTTAATGCTTTTAAGCATCATTTGTTGCACAAAACTAGCGCGGTGCTAGAAAATAATTTCTAGGAATGCATTTCTCAGGTGAAAATATCTTTACATACTTTCTCGATAATCTTCACAAAAACAATACTCGTAAACTTGTGAATGTTTTGTTCTTGAACAATTTATCCTTGAACATTATTTTCCCTCAATGTAAAATATTTGACCTGAGTTCTTCAAAGTGAGCTGTTTATCATGACCAGGGAATTATCTTTAATAGACTGATATATCTTAGTAATACATTTTCATCGACAACGACCAAGAATGAATAGCATTTTCTGCTCAAAACTGAGAAACATATGAAAAATGTAAAATTAATGCTTGTCCCATTTGACTAGGAGTTATCAATTTGTAGGTACATCATTTTTAGTTTTTGCGGGTGTGTTGTGGCCTTGTGCTGTTGTGCAGTCTGTTTGATATATTTTAATTTATTATATACAGATATCAGGTATTTATTGGTGGTACAAGACTGCTAGTCATGCTGCTGAATTAGCTGCCGGATATTATAATCCATGCAATCGTGATGGCTATGCTGCAATCACAGCAATGTTAAAGAGAAACGGTGTTAACTTAAACATTGCCTGTGTTGATTTGCACACATTCAATCAACACGAGAGCTTTCCAGAGCCATTTGCTGATCCCGAGCGATTAGTTTGGCAAGTCAGTATCTAATCCTTGATAAAATTTATCTTCTATCTAATTGGTTTCTTGCATAAGCAATTACACTAGGAAGAACTTATTAGAAGATTGGTCTTTGTTGTTCTATTTTAGACAAATTTGGGATGTGCCAAATACAGTTACAGAATAAAAGTTGTATGATCTAAAATACACATTGTGGGTATGTATTGCAGGTGTTGAATGCCGGGTGGGATGTTGGACTACCCGTCGTTAGTGAGAATGCACTTCCTTGCCTAAACAGAGTTAGCTATAACAAGGTTTTGGATAATACCAAGCCCATTAATGATCCAGATGGAAGGCACTTTTCATCCTTTACATACCTGAGGCTCAGTCCACTTCTGATGGAACGACCAAACTTCATAGAGTTTGAACGATTTGTCAAGAGGATGCACGGTAAGTTGATGCAACAAAGTTTTAAATACATCTCTAGCCTCTTGTGAAAAAATTTGTTAAGGTGAAAATAGTTCCTTTATCTGACATTGATTAAACGAATCAAATTAGAAGAATGGAAGTCCCAAATTTCTCTAATGGGCATAGTCTAGAAGCTTAAATACTTTTTCGATTTAACTTTACTTGTATTATTGGCTCCAAGGGATAGTGTCCTTTTATCCCCATGCATTCCACATTCAGTTCCCAACCTTTTGACAGCTAATCATTGCATGAACCTTTTATCTGATTGGATTTGGAATCATTTTATAAGTAAAACGATATCAAATGAGTTTTAATATCAGTTAACAGTTGCTAGGTTTTAGGGTTGATGTCATGATTGTGTCTTTTCTCACTGCAGGAGAAGCTGTTCTTGATCTTCAGGTATAA
Protein sequence

>MS.gene023885.t1

MVEIDGGSGMGTGAGDNRFQQHHEFQEQAGTPGGGNRRSRPVEEKERTKLRERRRRAITARILAGLRRHGNYNLRVRADINDVIAALAREAGWVVLPDGTTFPSKSQGQMPAGGNSAVVTSSSSHVPSQQPPSVSLKGVASGYGSPLDYNACPMKDVFIPSPSPYDLSSTSRSQTSMVGDGEAQRDNPPLIDGSIDTIREKQIADIPPRLPERDFAGSPYVPVYVMLPLGVINIKCELVDPDGILKQLRVLKSANVDGVMVDCWWGIVEAHAPQEYNWNGYKRLFQMVRELKLKLQVVMSFHECGGNFGDDVCIPLPHWIAEIGRSNPDIFFTDREGRHNPECLSWGIDKERVLRGRTAVEVYFDFMRSFRIEFNEYFEDGFISMIEVGLGPCGELRYPSCPVKHGWRYPGIGEFQCYDQYMLKSLRKAAEERGHTIWARGPDNVGTYNSQPHETGFFCDGGDYDGFYGRFFLNWYSQALVDHGNRVLSMAKLAFEGSCIAAKISGIYWWYKTASHAAELAAGYYNPCNRDGYAAITAMLKRNGVNLNIACVDLHTFNQHESFPEPFADPERLVWQVLNAGWDVGLPVVSENALPCLNRVSYNKVLDNTKPINDPDGRHFSSFTYLRLSPLLMERPNFIEFERFVKRMHGEAVLDLQV