AlfalfaGEDB | MS.gene023981

M. sativa cultivar XinJiangDaYe / MS.gene023981

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023981.t1	RHN47048.1	86.4	162	22	0	1	162	1	162	6.00E-71	276.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023981.t1	Q9FFS3	52.7	74	17	2	98	161	53	118	3.2e-12	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023981.t1	G7L6N9	87.0	161	21	0	1	161	1	161	4.3e-71	276.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene023981.t1	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023981.t1	MTR_7g079010	95.031	161	8	0	1	161	1	161	1.78e-112	318
MS.gene023981.t1	MTR_8g007270	65.116	43	15	0	119	161	132	174	1.52e-13	66.2
MS.gene023981.t1	MTR_7g028415	84.848	33	5	0	129	161	81	113	1.74e-13	65.1
MS.gene023981.t1	MTR_7g028710	84.848	33	5	0	129	161	81	113	1.74e-13	65.1
MS.gene023981.t1	MTR_3g095080	68.421	38	12	0	127	164	154	191	2.52e-13	65.1
MS.gene023981.t1	MTR_5g029850	69.231	39	12	0	119	157	29	67	1.56e-12	60.1
MS.gene023981.t1	MTR_5g043880	58.929	56	14	1	106	161	98	144	1.89e-12	63.9
MS.gene023981.t1	MTR_4g094588	80.000	35	7	0	127	161	164	198	1.98e-12	64.3
MS.gene023981.t1	MTR_2g033820	80.000	35	7	0	127	161	189	223	2.23e-12	64.3
MS.gene023981.t1	MTR_1g086790	70.000	40	12	0	122	161	139	178	4.24e-12	63.2
MS.gene023981.t1	MTR_5g091390	80.000	35	7	0	127	161	143	177	6.48e-12	62.8
MS.gene023981.t1	MTR_8g092140	69.444	36	10	1	127	161	145	180	1.91e-11	60.5
MS.gene023981.t1	MTR_3g095040	58.000	50	20	1	113	161	89	138	2.36e-11	60.1
MS.gene023981.t1	MTR_3g095080	68.571	35	11	0	127	161	154	188	2.57e-11	60.5
MS.gene023981.t1	MTR_3g095080	63.158	38	14	0	124	161	127	164	2.98e-11	60.1
MS.gene023981.t1	MTR_8g096730	51.786	56	24	1	106	161	38	90	4.67e-11	58.2
MS.gene023981.t1	MTR_7g109800	48.387	62	23	1	100	161	113	165	8.87e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene023981.t1	AT2G46130	69.565	46	14	0	122	167	11	56	4.02e-17	72.8
MS.gene023981.t1	AT5G41570	63.158	57	13	1	105	161	70	118	4.28e-17	74.7
MS.gene023981.t1	AT2G46130	75.000	40	10	0	122	161	11	50	7.06e-15	67.4
MS.gene023981.t1	AT1G64000	65.909	44	15	0	118	161	91	134	8.22e-15	69.3
MS.gene023981.t1	AT5G13080	85.294	34	5	0	128	161	54	87	1.61e-14	67.4
MS.gene023981.t1	AT2G03340	38.947	95	54	1	71	161	341	435	3.80e-13	66.6
MS.gene023981.t1	AT1G69310	44.186	86	42	1	82	167	94	173	4.01e-13	65.9
MS.gene023981.t1	AT3G01970	82.353	34	6	0	128	161	52	85	4.37e-13	63.5
MS.gene023981.t1	AT2G44745	60.465	43	17	0	119	161	123	165	4.95e-13	64.7
MS.gene023981.t1	AT1G69310	46.250	80	37	1	82	161	94	167	1.36e-12	64.3
MS.gene023981.t1	AT1G69310	46.250	80	37	1	82	161	94	167	1.36e-12	64.3
MS.gene023981.t1	AT1G69310	46.250	80	37	1	82	161	94	167	1.36e-12	64.3
MS.gene023981.t1	AT4G39410	52.083	48	23	0	114	161	196	243	2.37e-12	63.9
MS.gene023981.t1	AT5G49520	82.353	34	6	0	128	161	208	241	1.24e-11	62.0
MS.gene023981.t1	AT5G46350	34.899	149	71	4	28	161	66	203	1.38e-11	61.6
MS.gene023981.t1	AT4G18170	62.500	48	14	1	118	161	145	192	1.42e-11	61.6
MS.gene023981.t1	AT2G47260	51.613	62	21	1	100	161	142	194	5.68e-11	60.1
MS.gene023981.t1	AT2G38470	50.746	67	25	1	103	161	316	382	8.58e-11	59.7

Find 44 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCATCTTCTTCTAATT+TGG	0.109939	7.1:-24855970	MS.gene023981:CDS
AAGAAGTGTTGATGATATTT+TGG	0.221201	7.1:-24855817	MS.gene023981:CDS
ACAACAATAACTTGCTTGTT+GGG	0.273574	7.1:-24856034	MS.gene023981:CDS
ATTAATGGTGGTGGTGGGTT+TGG	0.274937	7.1:+24856197	None:intergenic
GGTGATGGTATATTCATTAA+TGG	0.285795	7.1:+24856182	None:intergenic
GTGGTGGGTTTGGTGGGTCT+TGG	0.304953	7.1:+24856207	None:intergenic
GGTTATCGTTGGAGGAAATA+TGG	0.317694	7.1:-24855789	MS.gene023981:CDS
AACAACAATAACTTGCTTGT+TGG	0.348151	7.1:-24856035	MS.gene023981:CDS
GAAACAAGTTAGAGGAGAAA+AGG	0.356863	7.1:-24855896	MS.gene023981:CDS
TTAGAGGAGAAAAGGGTTAG+AGG	0.362096	7.1:-24855888	MS.gene023981:CDS
AGAAAAGGGAAACAAGTTAG+AGG	0.399768	7.1:-24855904	MS.gene023981:CDS
TTCTTTACAAAACTACCCTT+TGG	0.400016	7.1:-24856114	MS.gene023981:CDS
TCACCTACATGAAGAAGAAA+AGG	0.429201	7.1:-24855919	MS.gene023981:CDS
AAACAAGTTAGAGGAGAAAA+GGG	0.432866	7.1:-24855895	MS.gene023981:CDS
ATATTCATTAATGGTGGTGG+TGG	0.437732	7.1:+24856191	None:intergenic
TAAAGAAGGTGTAAAGAAGA+AGG	0.451312	7.1:+24856130	None:intergenic
GAATCTCAAGATCAAGGCCT+TGG	0.455635	7.1:-24856092	MS.gene023981:CDS
ATGGTGGTGGTGGGTTTGGT+GGG	0.457794	7.1:+24856201	None:intergenic
TGGATGATGGTTATCGTTGG+AGG	0.458848	7.1:-24855797	MS.gene023981:CDS
AATGGTGGTGGTGGGTTTGG+TGG	0.469202	7.1:+24856200	None:intergenic
GTAATTCAAAATAATGAAAG+TGG	0.470864	7.1:-24855948	MS.gene023981:CDS
AAAGAAGGTGTAAAGAAGAA+GGG	0.479028	7.1:+24856131	None:intergenic
AGGAGAAAAGGGTTAGAGGT+GGG	0.487742	7.1:-24855884	MS.gene023981:CDS
CTTGCTTGTTGGGGATGCTA+AGG	0.508570	7.1:-24856024	MS.gene023981:CDS
ACATACATGTAGTATATACC+TGG	0.511478	7.1:+24855737	None:intergenic
TATTCATTAATGGTGGTGGT+GGG	0.530475	7.1:+24856192	None:intergenic
CACCTACATGAAGAAGAAAA+GGG	0.538463	7.1:-24855918	MS.gene023981:CDS
GAGGAGAAAAGGGTTAGAGG+TGG	0.538687	7.1:-24855885	MS.gene023981:CDS
CAACAATAACTTGCTTGTTG+GGG	0.541537	7.1:-24856033	MS.gene023981:CDS
AAGAAGGTGTAAAGAAGAAG+GGG	0.545916	7.1:+24856132	None:intergenic
CCTTTGGAATCTCAAGATCA+AGG	0.550549	7.1:-24856098	MS.gene023981:CDS
GGTATATTCATTAATGGTGG+TGG	0.558354	7.1:+24856188	None:intergenic
CTTGATCTTGAGATTCCAAA+GGG	0.558938	7.1:+24856099	None:intergenic
CTTGTCTGAAATGCAAACCT+TGG	0.566555	7.1:+24855837	None:intergenic
TCAAGAACAGCAAGTATCCC+AGG	0.569780	7.1:-24855755	MS.gene023981:CDS
AGGCCTTGGTGATATTGAGT+GGG	0.589628	7.1:-24856078	MS.gene023981:CDS
TAATTCAAAATAATGAAAGT+GGG	0.605922	7.1:-24855947	MS.gene023981:CDS
AAGGCCTTGGTGATATTGAG+TGG	0.607465	7.1:-24856079	MS.gene023981:CDS
CCTTGATCTTGAGATTCCAA+AGG	0.608143	7.1:+24856098	None:intergenic
GATGGTATATTCATTAATGG+TGG	0.631790	7.1:+24856185	None:intergenic
AAAAGACAACAAAAGTGCCA+AGG	0.641958	7.1:-24855854	MS.gene023981:CDS
CATACATGTAGTATATACCT+GGG	0.661629	7.1:+24855738	None:intergenic
GGCCTTGGTGATATTGAGTG+GGG	0.673375	7.1:-24856077	MS.gene023981:CDS
TTCCCCACTCAATATCACCA+AGG	0.720297	7.1:+24856075	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATTCAAAATAATGAAAGT+GGG	-	chr7.1:24855999-24856018	MS.gene023981:CDS	15.0%
!!	GTAATTCAAAATAATGAAAG+TGG	-	chr7.1:24855998-24856017	MS.gene023981:CDS	20.0%
!!!	AAGAAGTGTTGATGATATTT+TGG	-	chr7.1:24856129-24856148	MS.gene023981:CDS	25.0%
	AAACAAGTTAGAGGAGAAAA+GGG	-	chr7.1:24856051-24856070	MS.gene023981:CDS	30.0%
	ACATACATGTAGTATATACC+TGG	+	chr7.1:24856212-24856231	None:intergenic	30.0%
	CACTTCATCTTCTTCTAATT+TGG	-	chr7.1:24855976-24855995	MS.gene023981:CDS	30.0%
	CATACATGTAGTATATACCT+GGG	+	chr7.1:24856211-24856230	None:intergenic	30.0%
	GATGGTATATTCATTAATGG+TGG	+	chr7.1:24855764-24855783	None:intergenic	30.0%
	TTCTTTACAAAACTACCCTT+TGG	-	chr7.1:24855832-24855851	MS.gene023981:CDS	30.0%
!	AAAGAAGGTGTAAAGAAGAA+GGG	+	chr7.1:24855818-24855837	None:intergenic	30.0%
!	GGTGATGGTATATTCATTAA+TGG	+	chr7.1:24855767-24855786	None:intergenic	30.0%
!	TAAAGAAGGTGTAAAGAAGA+AGG	+	chr7.1:24855819-24855838	None:intergenic	30.0%
!!	AACAACAATAACTTGCTTGT+TGG	-	chr7.1:24855911-24855930	MS.gene023981:CDS	30.0%
!!	ACAACAATAACTTGCTTGTT+GGG	-	chr7.1:24855912-24855931	MS.gene023981:CDS	30.0%
!!!	AAAGGGTAGTTTTGTAAAGA+AGG	+	chr7.1:24855833-24855852	None:intergenic	30.0%
!!!	GTTGATGATATTTTGGATGA+TGG	-	chr7.1:24856136-24856155	MS.gene023981:CDS	30.0%
	AGAAAAGGGAAACAAGTTAG+AGG	-	chr7.1:24856042-24856061	MS.gene023981:CDS	35.0%
	CACCTACATGAAGAAGAAAA+GGG	-	chr7.1:24856028-24856047	MS.gene023981:CDS	35.0%
	CTTGATCTTGAGATTCCAAA+GGG	+	chr7.1:24855850-24855869	None:intergenic	35.0%
	GAAACAAGTTAGAGGAGAAA+AGG	-	chr7.1:24856050-24856069	MS.gene023981:CDS	35.0%
	GGTATATTCATTAATGGTGG+TGG	+	chr7.1:24855761-24855780	None:intergenic	35.0%
	TCACCTACATGAAGAAGAAA+AGG	-	chr7.1:24856027-24856046	MS.gene023981:CDS	35.0%
!	AAGAAGGTGTAAAGAAGAAG+GGG	+	chr7.1:24855817-24855836	None:intergenic	35.0%
!	TTCCCTTTTCTTCTTCATGT+AGG	+	chr7.1:24856033-24856052	None:intergenic	35.0%
!!	AAAAGACAACAAAAGTGCCA+AGG	-	chr7.1:24856092-24856111	MS.gene023981:CDS	35.0%
!!	ATATTCATTAATGGTGGTGG+TGG	+	chr7.1:24855758-24855777	None:intergenic	35.0%
!!	CAACAATAACTTGCTTGTTG+GGG	-	chr7.1:24855913-24855932	MS.gene023981:CDS	35.0%
!!	TATTCATTAATGGTGGTGGT+GGG	+	chr7.1:24855757-24855776	None:intergenic	35.0%
!!	TTTTGGATGATGGTTATCGT+TGG	-	chr7.1:24856146-24856165	MS.gene023981:CDS	35.0%
!!!	GTGTTGTTGTTTTGAGTTTG+TGG	+	chr7.1:24855788-24855807	None:intergenic	35.0%
!!!	TTGTTTTGAGTTTGTGGTGA+TGG	+	chr7.1:24855782-24855801	None:intergenic	35.0%
	CCTTGATCTTGAGATTCCAA+AGG	+	chr7.1:24855851-24855870	None:intergenic	40.0%
	GGTTATCGTTGGAGGAAATA+TGG	-	chr7.1:24856157-24856176	MS.gene023981:CDS	40.0%
	TTAGAGGAGAAAAGGGTTAG+AGG	-	chr7.1:24856058-24856077	MS.gene023981:CDS	40.0%
!	CTTGTCTGAAATGCAAACCT+TGG	+	chr7.1:24856112-24856131	None:intergenic	40.0%
!!	CCTTTGGAATCTCAAGATCA+AGG	-	chr7.1:24855848-24855867	MS.gene023981:CDS	40.0%
!!	GAGTGGGGAAACTTTTTCTT+AGG	-	chr7.1:24855884-24855903	MS.gene023981:CDS	40.0%
	AGGAGAAAAGGGTTAGAGGT+GGG	-	chr7.1:24856062-24856081	MS.gene023981:CDS	45.0%
	GAATCTCAAGATCAAGGCCT+TGG	-	chr7.1:24855854-24855873	MS.gene023981:CDS	45.0%
	TCAAGAACAGCAAGTATCCC+AGG	-	chr7.1:24856191-24856210	MS.gene023981:CDS	45.0%
	TTCCCCACTCAATATCACCA+AGG	+	chr7.1:24855874-24855893	None:intergenic	45.0%
!	AAGGCCTTGGTGATATTGAG+TGG	-	chr7.1:24855867-24855886	MS.gene023981:CDS	45.0%
!	AGGCCTTGGTGATATTGAGT+GGG	-	chr7.1:24855868-24855887	MS.gene023981:CDS	45.0%
!	TGGATGATGGTTATCGTTGG+AGG	-	chr7.1:24856149-24856168	MS.gene023981:CDS	45.0%
!!	ATTAATGGTGGTGGTGGGTT+TGG	+	chr7.1:24855752-24855771	None:intergenic	45.0%
	GAGGAGAAAAGGGTTAGAGG+TGG	-	chr7.1:24856061-24856080	MS.gene023981:CDS	50.0%
!	CTTGCTTGTTGGGGATGCTA+AGG	-	chr7.1:24855922-24855941	MS.gene023981:CDS	50.0%
!	GGCCTTGGTGATATTGAGTG+GGG	-	chr7.1:24855869-24855888	MS.gene023981:CDS	50.0%
!!	AATGGTGGTGGTGGGTTTGG+TGG	+	chr7.1:24855749-24855768	None:intergenic	55.0%
!!	ATGGTGGTGGTGGGTTTGGT+GGG	+	chr7.1:24855748-24855767	None:intergenic	55.0%
!!	GTGGTGGGTTTGGTGGGTCT+TGG	+	chr7.1:24855742-24855761	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	24855731	24856237	24855731	ID=MS.gene023981
chr7.1	mRNA	24855731	24856237	24855731	ID=MS.gene023981.t1;Parent=MS.gene023981
chr7.1	exon	24855731	24856237	24855731	ID=MS.gene023981.t1.exon1;Parent=MS.gene023981.t1
chr7.1	CDS	24855731	24856237	24855731	ID=cds.MS.gene023981.t1;Parent=MS.gene023981.t1

Gene Sequence

>MS.gene023981

ATGGATAGCCAAGACCCACCAAACCCACCACCACCATTAATGAATATACCATCACCACAAACTCAAAACAACAACACTTCTCTCCCCTTCTTCTTTACACCTTCTTTACAAAACTACCCTTTGGAATCTCAAGATCAAGGCCTTGGTGATATTGAGTGGGGAAACTTTTTCTTAGGTCAAAACAACAATAACTTGCTTGTTGGGGATGCTAAGGAGACATTAAAGATTGACAATATTCAACAATGCACTTCATCTTCTTCTAATTTGGTAATTCAAAATAATGAAAGTGGGAGTTATCACCTACATGAAGAAGAAAAGGGAAACAAGTTAGAGGAGAAAAGGGTTAGAGGTGGGAGAGTGAAAAAGACAACAAAAGTGCCAAGGTTTGCATTTCAGACAAGAAGTGTTGATGATATTTTGGATGATGGTTATCGTTGGAGGAAATATGGACAAAAAGCTGTCAAGAACAGCAAGTATCCCAGGTATATACTACATGTATGTTTGTGA

Protein sequence

>MS.gene023981.t1

MDSQDPPNPPPPLMNIPSPQTQNNNTSLPFFFTPSLQNYPLESQDQGLGDIEWGNFFLGQNNNNLLVGDAKETLKIDNIQQCTSSSSNLVIQNNESGSYHLHEEEKGNKLEEKRVRGGRVKKTTKVPRFAFQTRSVDDILDDGYRWRKYGQKAVKNSKYPRYILHVCL