Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024142.t1 | XP_013460954.1 | 87 | 46 | 6 | 0 | 1 | 46 | 15 | 60 | 1.30E-14 | 88.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024142.t1 | Q9STM3 | 63.0 | 46 | 17 | 0 | 1 | 46 | 17 | 62 | 2.6e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024142.t1 | A0A072V0U6 | 87.0 | 46 | 6 | 0 | 1 | 46 | 15 | 60 | 9.5e-15 | 88.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene024142.t1 | TR | Jumonji |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024142.t1 | MTR_3g075210 | 86.957 | 46 | 6 | 0 | 1 | 46 | 15 | 60 | 8.29e-24 | 92.8 |
| MS.gene024142.t1 | MTR_1g090763 | 90.244 | 41 | 4 | 0 | 1 | 41 | 1 | 41 | 3.42e-21 | 80.5 |
| MS.gene024142.t1 | MTR_1g094740 | 66.667 | 45 | 14 | 1 | 1 | 44 | 13 | 57 | 1.57e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024142.t1 | AT5G04240 | 64.444 | 45 | 15 | 1 | 1 | 44 | 13 | 57 | 7.22e-14 | 64.7 |
| MS.gene024142.t1 | AT3G48430 | 69.444 | 36 | 11 | 0 | 1 | 36 | 17 | 52 | 3.09e-13 | 63.2 |
Find 28 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACCCTTTGGCCCTTGAATT+TGG | 0.187974 | 7.1:-23155439 | None:intergenic |
| AGCTGGAGTGGGTCGGTATT+CGG | 0.239347 | 7.1:-23155325 | None:intergenic |
| GCTGGAGTGGGTCGGTATTC+GGG | 0.344330 | 7.1:-23155324 | None:intergenic |
| GAAAACCAAGCTTCCAAATT+CGG | 0.381270 | 7.1:+23155384 | MS.gene024142:CDS |
| GGGCCAAAGGGTCTTTGTTA+AGG | 0.400580 | 7.1:+23155449 | MS.gene024142:CDS |
| GGTCGGTATTCGGGTGCTAC+TGG | 0.434393 | 7.1:-23155315 | None:intergenic |
| GTTTATGAAGGAGGAGATAC+CGG | 0.440507 | 7.1:+23155471 | MS.gene024142:CDS |
| ATCTTGAAAATGTATTCGAT+TGG | 0.441436 | 7.1:-23155360 | None:intergenic |
| GTCTTTGTTAAGGTTTATGA+AGG | 0.445798 | 7.1:+23155459 | MS.gene024142:CDS |
| CTTTGGCCCTTGAATTTGGA+AGG | 0.452571 | 7.1:-23155435 | None:intergenic |
| ATTGGATCTTCGAATTCAGC+TGG | 0.456740 | 7.1:-23155342 | None:intergenic |
| AGAGTCCGAATTTGGAAGCT+TGG | 0.462536 | 7.1:-23155389 | None:intergenic |
| AAACCTTAACAAAGACCCTT+TGG | 0.469682 | 7.1:-23155452 | None:intergenic |
| ACCGGGAGTTACTTCGCCTA+TGG | 0.476123 | 7.1:+23155489 | MS.gene024142:CDS |
| TTTGTTAAGGTTTATGAAGG+AGG | 0.495534 | 7.1:+23155462 | MS.gene024142:CDS |
| AATTTGGAAGGTGGAAAATG+AGG | 0.514916 | 7.1:-23155423 | None:intergenic |
| TTCCAAATTCAAGGGCCAAA+GGG | 0.528642 | 7.1:+23155437 | MS.gene024142:CDS |
| ATCTTCGAATTCAGCTGGAG+TGG | 0.545061 | 7.1:-23155337 | None:intergenic |
| ACCATAGGCGAAGTAACTCC+CGG | 0.548529 | 7.1:-23155490 | None:intergenic |
| GTATTCGGGTGCTACTGGCA+TGG | 0.554461 | 7.1:-23155310 | None:intergenic |
| TTTCCACCTTCCAAATTCAA+GGG | 0.561710 | 7.1:+23155429 | MS.gene024142:CDS |
| CTTCCAAATTCAAGGGCCAA+AGG | 0.564784 | 7.1:+23155436 | MS.gene024142:CDS |
| ATTTGGAAGGTGGAAAATGA+GGG | 0.566469 | 7.1:-23155422 | None:intergenic |
| TCTTCGAATTCAGCTGGAGT+GGG | 0.577536 | 7.1:-23155336 | None:intergenic |
| TTTATGAAGGAGGAGATACC+GGG | 0.596623 | 7.1:+23155472 | MS.gene024142:CDS |
| CGAATTCAGCTGGAGTGGGT+CGG | 0.600042 | 7.1:-23155332 | None:intergenic |
| TGGCCCTTGAATTTGGAAGG+TGG | 0.617375 | 7.1:-23155432 | None:intergenic |
| AGCATGGCAAGATAAACCAT+AGG | 0.687611 | 7.1:-23155505 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCTTGAAAATGTATTCGAT+TGG | - | chr7.1:23155363-23155382 | None:intergenic | 25.0% |
| GTCTTTGTTAAGGTTTATGA+AGG | + | chr7.1:23155459-23155478 | MS.gene024142:CDS | 30.0% | |
| TTTGTTAAGGTTTATGAAGG+AGG | + | chr7.1:23155462-23155481 | MS.gene024142:CDS | 30.0% | |
| AAACCTTAACAAAGACCCTT+TGG | - | chr7.1:23155455-23155474 | None:intergenic | 35.0% | |
| AATTTGGAAGGTGGAAAATG+AGG | - | chr7.1:23155426-23155445 | None:intergenic | 35.0% | |
| ATTTGGAAGGTGGAAAATGA+GGG | - | chr7.1:23155425-23155444 | None:intergenic | 35.0% | |
| GAAAACCAAGCTTCCAAATT+CGG | + | chr7.1:23155384-23155403 | MS.gene024142:CDS | 35.0% | |
| TTTCCACCTTCCAAATTCAA+GGG | + | chr7.1:23155429-23155448 | MS.gene024142:CDS | 35.0% | |
| ! | TTTTCCACCTTCCAAATTCA+AGG | + | chr7.1:23155428-23155447 | MS.gene024142:CDS | 35.0% |
| AGCATGGCAAGATAAACCAT+AGG | - | chr7.1:23155508-23155527 | None:intergenic | 40.0% | |
| ATTGGATCTTCGAATTCAGC+TGG | - | chr7.1:23155345-23155364 | None:intergenic | 40.0% | |
| GTTTATGAAGGAGGAGATAC+CGG | + | chr7.1:23155471-23155490 | MS.gene024142:CDS | 40.0% | |
| TTCCAAATTCAAGGGCCAAA+GGG | + | chr7.1:23155437-23155456 | MS.gene024142:CDS | 40.0% | |
| TTTATGAAGGAGGAGATACC+GGG | + | chr7.1:23155472-23155491 | MS.gene024142:CDS | 40.0% | |
| ! | GATTTTGCAGAGTCCGAATT+TGG | - | chr7.1:23155400-23155419 | None:intergenic | 40.0% |
| AGAGTCCGAATTTGGAAGCT+TGG | - | chr7.1:23155392-23155411 | None:intergenic | 45.0% | |
| ATCTTCGAATTCAGCTGGAG+TGG | - | chr7.1:23155340-23155359 | None:intergenic | 45.0% | |
| CTTCCAAATTCAAGGGCCAA+AGG | + | chr7.1:23155436-23155455 | MS.gene024142:CDS | 45.0% | |
| CTTTGGCCCTTGAATTTGGA+AGG | - | chr7.1:23155438-23155457 | None:intergenic | 45.0% | |
| TCTTCGAATTCAGCTGGAGT+GGG | - | chr7.1:23155339-23155358 | None:intergenic | 45.0% | |
| ACCATAGGCGAAGTAACTCC+CGG | - | chr7.1:23155493-23155512 | None:intergenic | 50.0% | |
| GACCCTTTGGCCCTTGAATT+TGG | - | chr7.1:23155442-23155461 | None:intergenic | 50.0% | |
| GGGCCAAAGGGTCTTTGTTA+AGG | + | chr7.1:23155449-23155468 | MS.gene024142:CDS | 50.0% | |
| TGGCCCTTGAATTTGGAAGG+TGG | - | chr7.1:23155435-23155454 | None:intergenic | 50.0% | |
| ACCGGGAGTTACTTCGCCTA+TGG | + | chr7.1:23155489-23155508 | MS.gene024142:CDS | 55.0% | |
| AGCTGGAGTGGGTCGGTATT+CGG | - | chr7.1:23155328-23155347 | None:intergenic | 55.0% | |
| CGAATTCAGCTGGAGTGGGT+CGG | - | chr7.1:23155335-23155354 | None:intergenic | 55.0% | |
| GCTGGAGTGGGTCGGTATTC+GGG | - | chr7.1:23155327-23155346 | None:intergenic | 60.0% | |
| ! | GGTCGGTATTCGGGTGCTAC+TGG | - | chr7.1:23155318-23155337 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 23155312 | 23155533 | 23155312 | ID=MS.gene024142 |
| chr7.1 | mRNA | 23155312 | 23155533 | 23155312 | ID=MS.gene024142.t1;Parent=MS.gene024142 |
| chr7.1 | exon | 23155312 | 23155533 | 23155312 | ID=MS.gene024142.t1.exon1;Parent=MS.gene024142.t1 |
| chr7.1 | CDS | 23155312 | 23155533 | 23155312 | ID=cds.MS.gene024142.t1;Parent=MS.gene024142.t1 |
| Gene Sequence |
| Protein sequence |
