Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024143.t1 | XP_003624327.1 | 95.6 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 3.10E-55 | 224.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024143.t1 | Q8VZG8 | 52.9 | 87 | 36 | 2 | 1 | 86 | 955 | 1037 | 4.2e-16 | 85.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024143.t1 | G7KU95 | 95.6 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 2.3e-55 | 224.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024143.t1 | MTR_7g081780 | 95.614 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 2.31e-71 | 229 |
| MS.gene024143.t1 | MTR_7g081480 | 88.393 | 112 | 13 | 0 | 1 | 112 | 949 | 1060 | 8.68e-64 | 208 |
| MS.gene024143.t1 | MTR_7g481630 | 77.876 | 113 | 24 | 1 | 1 | 112 | 80 | 192 | 7.85e-59 | 179 |
| MS.gene024143.t1 | MTR_7g481590 | 77.876 | 113 | 24 | 1 | 1 | 112 | 203 | 315 | 7.29e-57 | 178 |
| MS.gene024143.t1 | MTR_7g081570 | 76.991 | 113 | 25 | 1 | 1 | 112 | 939 | 1051 | 1.09e-53 | 180 |
| MS.gene024143.t1 | MTR_7g081410 | 75.221 | 113 | 27 | 1 | 1 | 112 | 826 | 938 | 7.54e-53 | 177 |
| MS.gene024143.t1 | MTR_7g081410 | 75.221 | 113 | 27 | 1 | 1 | 112 | 946 | 1058 | 9.29e-53 | 177 |
| MS.gene024143.t1 | MTR_7g081720 | 53.509 | 114 | 51 | 1 | 1 | 112 | 964 | 1077 | 6.23e-32 | 118 |
| MS.gene024143.t1 | MTR_5g085700 | 54.464 | 112 | 51 | 0 | 2 | 113 | 921 | 1032 | 1.06e-31 | 117 |
| MS.gene024143.t1 | MTR_8g445960 | 49.000 | 100 | 50 | 1 | 1 | 99 | 63 | 162 | 5.54e-29 | 103 |
| MS.gene024143.t1 | MTR_8g044940 | 49.000 | 100 | 50 | 1 | 1 | 99 | 63 | 162 | 5.54e-29 | 103 |
| MS.gene024143.t1 | MTR_1g039080 | 52.525 | 99 | 46 | 1 | 1 | 98 | 192 | 290 | 5.28e-28 | 103 |
| MS.gene024143.t1 | MTR_1g039310 | 48.246 | 114 | 55 | 2 | 3 | 112 | 1051 | 1164 | 2.58e-27 | 105 |
| MS.gene024143.t1 | MTR_1g038890 | 52.525 | 99 | 46 | 1 | 1 | 98 | 1033 | 1131 | 4.07e-27 | 104 |
| MS.gene024143.t1 | MTR_7g045510 | 53.261 | 92 | 41 | 1 | 2 | 93 | 1091 | 1180 | 2.55e-25 | 99.4 |
| MS.gene024143.t1 | MTR_7g039330 | 53.261 | 92 | 41 | 1 | 2 | 93 | 1189 | 1278 | 4.86e-25 | 98.6 |
| MS.gene024143.t1 | MTR_7g007540 | 43.860 | 114 | 59 | 2 | 1 | 113 | 1 | 110 | 2.86e-24 | 89.7 |
| MS.gene024143.t1 | MTR_7g007560 | 45.614 | 114 | 57 | 2 | 1 | 113 | 653 | 762 | 5.95e-24 | 95.5 |
| MS.gene024143.t1 | MTR_7g009940 | 44.248 | 113 | 58 | 2 | 3 | 114 | 700 | 808 | 4.54e-23 | 92.8 |
| MS.gene024143.t1 | MTR_7g045710 | 50.000 | 94 | 42 | 1 | 2 | 95 | 972 | 1060 | 5.00e-23 | 92.8 |
| MS.gene024143.t1 | MTR_1g039240 | 47.423 | 97 | 47 | 1 | 1 | 93 | 1058 | 1154 | 5.59e-23 | 92.8 |
| MS.gene024143.t1 | MTR_7g446160 | 52.128 | 94 | 44 | 1 | 1 | 93 | 887 | 980 | 9.53e-23 | 92.0 |
| MS.gene024143.t1 | MTR_0400s0040 | 47.423 | 97 | 47 | 1 | 1 | 93 | 1058 | 1154 | 1.84e-22 | 91.3 |
| MS.gene024143.t1 | MTR_0365s0030 | 47.423 | 97 | 47 | 1 | 1 | 93 | 1058 | 1154 | 1.84e-22 | 91.3 |
| MS.gene024143.t1 | MTR_0400s0030 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 2.29e-22 | 90.9 |
| MS.gene024143.t1 | MTR_0365s0020 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 2.29e-22 | 90.9 |
| MS.gene024143.t1 | MTR_7g050990 | 50.515 | 97 | 44 | 2 | 1 | 93 | 819 | 915 | 2.48e-22 | 90.9 |
| MS.gene024143.t1 | MTR_7g051040 | 43.478 | 115 | 56 | 2 | 1 | 108 | 843 | 955 | 4.01e-22 | 90.1 |
| MS.gene024143.t1 | MTR_7g045860 | 48.454 | 97 | 46 | 2 | 2 | 97 | 984 | 1077 | 4.85e-22 | 90.1 |
| MS.gene024143.t1 | MTR_1g040525 | 48.958 | 96 | 43 | 3 | 1 | 91 | 767 | 861 | 1.41e-21 | 88.6 |
| MS.gene024143.t1 | MTR_7g007630 | 43.860 | 114 | 59 | 2 | 1 | 113 | 666 | 775 | 1.96e-21 | 88.2 |
| MS.gene024143.t1 | MTR_1g040545 | 50.000 | 96 | 42 | 3 | 1 | 91 | 907 | 1001 | 2.75e-21 | 87.8 |
| MS.gene024143.t1 | MTR_7g009970 | 43.103 | 116 | 57 | 3 | 1 | 113 | 669 | 778 | 4.15e-21 | 87.4 |
| MS.gene024143.t1 | MTR_7g407130 | 43.363 | 113 | 59 | 2 | 1 | 112 | 696 | 804 | 1.39e-20 | 85.9 |
| MS.gene024143.t1 | MTR_1g040615 | 47.423 | 97 | 47 | 3 | 1 | 93 | 2024 | 2120 | 1.63e-20 | 85.5 |
| MS.gene024143.t1 | MTR_1g040615 | 44.545 | 110 | 49 | 5 | 1 | 103 | 1025 | 1129 | 5.65e-20 | 84.0 |
| MS.gene024143.t1 | MTR_1g039220 | 48.421 | 95 | 45 | 3 | 1 | 91 | 1032 | 1126 | 3.26e-20 | 84.7 |
| MS.gene024143.t1 | MTR_4g037720 | 44.086 | 93 | 48 | 1 | 1 | 93 | 848 | 936 | 2.71e-19 | 82.0 |
| MS.gene024143.t1 | MTR_7g407090 | 44.737 | 114 | 58 | 2 | 1 | 113 | 651 | 760 | 3.12e-19 | 82.0 |
| MS.gene024143.t1 | MTR_7g010000 | 41.379 | 116 | 59 | 3 | 1 | 113 | 746 | 855 | 5.63e-19 | 81.3 |
| MS.gene024143.t1 | MTR_4g037015 | 43.011 | 93 | 49 | 1 | 1 | 93 | 777 | 865 | 5.97e-19 | 81.3 |
| MS.gene024143.t1 | MTR_7g013680 | 41.026 | 117 | 60 | 3 | 1 | 114 | 619 | 729 | 6.96e-19 | 80.9 |
| MS.gene024143.t1 | MTR_7g028432 | 43.011 | 93 | 49 | 1 | 1 | 93 | 853 | 941 | 7.15e-19 | 80.9 |
| MS.gene024143.t1 | MTR_7g007620 | 45.614 | 114 | 57 | 2 | 1 | 113 | 620 | 729 | 9.40e-19 | 80.5 |
| MS.gene024143.t1 | MTR_7g096980 | 40.206 | 97 | 57 | 1 | 1 | 97 | 1108 | 1203 | 3.68e-18 | 79.0 |
| MS.gene024143.t1 | MTR_8g047230 | 51.087 | 92 | 40 | 2 | 1 | 91 | 875 | 962 | 4.86e-17 | 75.9 |
| MS.gene024143.t1 | MTR_0491s0030 | 51.087 | 92 | 40 | 2 | 1 | 91 | 704 | 791 | 1.02e-16 | 74.7 |
| MS.gene024143.t1 | MTR_8g047160 | 52.874 | 87 | 36 | 2 | 1 | 86 | 531 | 613 | 1.10e-16 | 74.7 |
| MS.gene024143.t1 | MTR_7g013350 | 43.689 | 103 | 49 | 3 | 1 | 100 | 295 | 391 | 2.51e-16 | 73.6 |
| MS.gene024143.t1 | MTR_7g446190 | 46.429 | 84 | 38 | 2 | 1 | 80 | 815 | 895 | 1.07e-15 | 72.0 |
| MS.gene024143.t1 | MTR_8g047210 | 51.087 | 92 | 40 | 2 | 1 | 91 | 768 | 855 | 1.76e-15 | 71.2 |
| MS.gene024143.t1 | MTR_8g047220 | 51.087 | 92 | 40 | 2 | 1 | 91 | 831 | 918 | 1.89e-15 | 71.2 |
| MS.gene024143.t1 | MTR_2g070020 | 38.000 | 100 | 51 | 2 | 1 | 93 | 987 | 1082 | 1.22e-13 | 65.9 |
| MS.gene024143.t1 | MTR_7g010010 | 36.207 | 116 | 46 | 3 | 1 | 113 | 616 | 706 | 4.18e-13 | 64.3 |
| MS.gene024143.t1 | MTR_7g022160 | 36.449 | 107 | 63 | 2 | 1 | 104 | 909 | 1013 | 4.77e-13 | 64.3 |
| MS.gene024143.t1 | MTR_3g047890 | 36.036 | 111 | 51 | 4 | 3 | 102 | 360 | 461 | 6.59e-12 | 60.8 |
| MS.gene024143.t1 | MTR_7g022050 | 38.542 | 96 | 54 | 2 | 1 | 93 | 534 | 627 | 1.27e-11 | 60.1 |
| MS.gene024143.t1 | MTR_3g093930 | 34.314 | 102 | 62 | 3 | 1 | 99 | 879 | 978 | 3.04e-11 | 59.3 |
| MS.gene024143.t1 | MTR_1g047670 | 36.000 | 100 | 55 | 3 | 1 | 93 | 989 | 1086 | 3.18e-11 | 58.9 |
| MS.gene024143.t1 | MTR_1g080440 | 31.000 | 100 | 66 | 1 | 1 | 97 | 888 | 987 | 6.19e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024143.t1 | AT4G08850 | 52.874 | 87 | 36 | 2 | 1 | 86 | 955 | 1037 | 1.67e-19 | 82.8 |
| MS.gene024143.t1 | AT1G35710 | 44.186 | 86 | 44 | 1 | 1 | 86 | 1031 | 1112 | 1.73e-15 | 71.2 |
| MS.gene024143.t1 | AT1G17230 | 38.000 | 100 | 51 | 2 | 1 | 93 | 977 | 1072 | 1.23e-12 | 63.2 |
| MS.gene024143.t1 | AT1G17230 | 38.000 | 100 | 51 | 2 | 1 | 93 | 977 | 1072 | 1.25e-12 | 63.2 |
| MS.gene024143.t1 | AT4G25390 | 37.755 | 98 | 51 | 2 | 3 | 93 | 530 | 624 | 9.89e-12 | 60.5 |
| MS.gene024143.t1 | AT3G28040 | 34.579 | 107 | 65 | 2 | 1 | 104 | 908 | 1012 | 1.17e-11 | 60.5 |
| MS.gene024143.t1 | AT2G33170 | 36.634 | 101 | 52 | 3 | 1 | 93 | 1003 | 1099 | 5.27e-11 | 58.5 |
| MS.gene024143.t1 | AT2G33170 | 36.634 | 101 | 52 | 3 | 1 | 93 | 1003 | 1099 | 5.27e-11 | 58.5 |
| MS.gene024143.t1 | AT1G09440 | 35.354 | 99 | 50 | 3 | 3 | 93 | 336 | 428 | 6.56e-11 | 58.2 |
| MS.gene024143.t1 | AT1G09440 | 35.354 | 99 | 50 | 3 | 3 | 93 | 336 | 428 | 6.56e-11 | 58.2 |
| MS.gene024143.t1 | AT4G31100 | 35.556 | 90 | 51 | 2 | 5 | 89 | 625 | 712 | 6.75e-11 | 58.2 |
| MS.gene024143.t1 | AT5G56790 | 36.458 | 96 | 55 | 2 | 2 | 93 | 567 | 660 | 7.40e-11 | 58.2 |
Find 23 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGCTTTGGAAATCATAA+AGG | 0.335311 | 7.1:-23148232 | MS.gene024143:CDS |
| AAATGTGATGTATACAGTTT+TGG | 0.382117 | 7.1:-23148258 | MS.gene024143:CDS |
| GTTGGCCGAGAGCGTGGCTC+TGG | 0.409601 | 7.1:+23148042 | None:intergenic |
| AAGATAACCTCCTCATCAAT+TGG | 0.413575 | 7.1:+23148096 | None:intergenic |
| CTTGCTTTGGAAATCATAAA+GGG | 0.419654 | 7.1:-23148231 | MS.gene024143:CDS |
| TGGAGCAGGAAAATCACCTT+TGG | 0.448518 | 7.1:-23147995 | MS.gene024143:CDS |
| TTAGCCAATGGCCTTGTAGA+TGG | 0.459178 | 7.1:+23148171 | None:intergenic |
| ATCATAAAGGGAGAACATCC+TGG | 0.483511 | 7.1:-23148219 | MS.gene024143:CDS |
| CAAATTCACACGATCAAACT+AGG | 0.483867 | 7.1:-23147967 | MS.gene024143:CDS |
| TATAATGAAATGAGATCTCC+AGG | 0.486322 | 7.1:+23148201 | None:intergenic |
| GATCAAGTATGTAAGATGCT+TGG | 0.514611 | 7.1:-23148015 | MS.gene024143:CDS |
| GTCACCATCTACAAGGCCAT+TGG | 0.522420 | 7.1:-23148175 | MS.gene024143:CDS |
| GTATGTAAGATGCTTGGAGC+AGG | 0.525016 | 7.1:-23148009 | MS.gene024143:CDS |
| GCCACGCTCTCGGCCAACCA+TGG | 0.525673 | 7.1:-23148037 | MS.gene024143:CDS |
| CATCTTACATACTTGATCCA+TGG | 0.528171 | 7.1:+23148020 | None:intergenic |
| TATATTTGTCACCATCTACA+AGG | 0.550499 | 7.1:-23148182 | MS.gene024143:CDS |
| TCCATGGTTGGCCGAGAGCG+TGG | 0.556917 | 7.1:+23148036 | None:intergenic |
| TAAATCCAGAGCCACGCTCT+CGG | 0.571908 | 7.1:-23148047 | MS.gene024143:CDS |
| TTACATACTTGATCCATGGT+TGG | 0.575557 | 7.1:+23148024 | None:intergenic |
| TATGAAACCAATTGATGAGG+AGG | 0.576165 | 7.1:-23148103 | MS.gene024143:CDS |
| GTGTGAATTTGATCTTCCAA+AGG | 0.599159 | 7.1:+23147979 | None:intergenic |
| AATAATGTATCATTAGCCAA+TGG | 0.628834 | 7.1:+23148159 | None:intergenic |
| AGTTATGAAACCAATTGATG+AGG | 0.632329 | 7.1:-23148106 | MS.gene024143:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAATGTATCATTAGCCAA+TGG | + | chr7.1:23148068-23148087 | None:intergenic | 25.0% |
| !! | AAATGTGATGTATACAGTTT+TGG | - | chr7.1:23147966-23147985 | MS.gene024143:CDS | 25.0% |
| !!! | ATTGGTTTCATAACTTTTTG+AGG | + | chr7.1:23148113-23148132 | None:intergenic | 25.0% |
| AGTTATGAAACCAATTGATG+AGG | - | chr7.1:23148118-23148137 | MS.gene024143:CDS | 30.0% | |
| TATAATGAAATGAGATCTCC+AGG | + | chr7.1:23148026-23148045 | None:intergenic | 30.0% | |
| TATATTTGTCACCATCTACA+AGG | - | chr7.1:23148042-23148061 | MS.gene024143:CDS | 30.0% | |
| !! | ACTTGCTTTGGAAATCATAA+AGG | - | chr7.1:23147992-23148011 | MS.gene024143:CDS | 30.0% |
| !! | CTTGCTTTGGAAATCATAAA+GGG | - | chr7.1:23147993-23148012 | MS.gene024143:CDS | 30.0% |
| AAGATAACCTCCTCATCAAT+TGG | + | chr7.1:23148131-23148150 | None:intergenic | 35.0% | |
| CAAATTCACACGATCAAACT+AGG | - | chr7.1:23148257-23148276 | MS.gene024143:CDS | 35.0% | |
| CATCTTACATACTTGATCCA+TGG | + | chr7.1:23148207-23148226 | None:intergenic | 35.0% | |
| GATCAAGTATGTAAGATGCT+TGG | - | chr7.1:23148209-23148228 | MS.gene024143:CDS | 35.0% | |
| GTGTGAATTTGATCTTCCAA+AGG | + | chr7.1:23148248-23148267 | None:intergenic | 35.0% | |
| TATGAAACCAATTGATGAGG+AGG | - | chr7.1:23148121-23148140 | MS.gene024143:CDS | 35.0% | |
| TTACATACTTGATCCATGGT+TGG | + | chr7.1:23148203-23148222 | None:intergenic | 35.0% | |
| ATCATAAAGGGAGAACATCC+TGG | - | chr7.1:23148005-23148024 | MS.gene024143:CDS | 40.0% | |
| !!! | CAGTTTTGGAGTACTTGCTT+TGG | - | chr7.1:23147980-23147999 | MS.gene024143:CDS | 40.0% |
| GTATGTAAGATGCTTGGAGC+AGG | - | chr7.1:23148215-23148234 | MS.gene024143:CDS | 45.0% | |
| TGGAGCAGGAAAATCACCTT+TGG | - | chr7.1:23148229-23148248 | MS.gene024143:CDS | 45.0% | |
| !! | TTAGCCAATGGCCTTGTAGA+TGG | + | chr7.1:23148056-23148075 | None:intergenic | 45.0% |
| GTCACCATCTACAAGGCCAT+TGG | - | chr7.1:23148049-23148068 | MS.gene024143:CDS | 50.0% | |
| TAAATCCAGAGCCACGCTCT+CGG | - | chr7.1:23148177-23148196 | MS.gene024143:CDS | 50.0% | |
| ! | TCCATGGTTGGCCGAGAGCG+TGG | + | chr7.1:23148191-23148210 | None:intergenic | 65.0% |
| GCCACGCTCTCGGCCAACCA+TGG | - | chr7.1:23148187-23148206 | MS.gene024143:CDS | 70.0% | |
| ! | GTTGGCCGAGAGCGTGGCTC+TGG | + | chr7.1:23148185-23148204 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 23147951 | 23148295 | 23147951 | ID=MS.gene024143 |
| chr7.1 | mRNA | 23147951 | 23148295 | 23147951 | ID=MS.gene024143.t1;Parent=MS.gene024143 |
| chr7.1 | exon | 23147951 | 23148295 | 23147951 | ID=MS.gene024143.t1.exon1;Parent=MS.gene024143.t1 |
| chr7.1 | CDS | 23147951 | 23148295 | 23147951 | ID=cds.MS.gene024143.t1;Parent=MS.gene024143.t1 |
| Gene Sequence |
| Protein sequence |
