AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024422

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024422.t1 MTR_7g117705 83.406 458 35 7 1 444 1 431 0.0 707
MS.gene024422.t1 MTR_1g013180 64.327 513 112 11 1 443 152 663 0.0 582
MS.gene024422.t1 MTR_1g013170 61.396 487 105 9 1 405 1 486 2.15e-180 515
MS.gene024422.t1 MTR_3g106220 64.444 315 103 4 3 311 6 317 2.78e-127 385
MS.gene024422.t1 MTR_2g450070 47.668 386 165 10 3 359 16 393 4.56e-98 310
MS.gene024422.t1 MTR_2g450070 47.668 386 165 10 3 359 16 393 6.08e-98 310
MS.gene024422.t1 MTR_4g131580 48.378 339 160 6 5 338 17 345 6.09e-89 285
MS.gene024422.t1 MTR_2g034960 54.264 258 115 1 1 258 25 279 1.57e-86 278
MS.gene024422.t1 MTR_4g131600 47.416 329 155 6 1 318 16 337 7.34e-86 276
MS.gene024422.t1 MTR_4g121020 52.249 289 121 5 2 277 28 312 6.73e-85 276
MS.gene024422.t1 MTR_4g121020 52.249 289 121 5 2 277 28 312 7.02e-85 276
MS.gene024422.t1 MTR_4g131570 44.384 365 158 9 3 343 15 358 1.55e-84 273
MS.gene024422.t1 MTR_1g032570 52.672 262 108 3 4 255 10 265 1.48e-82 268
MS.gene024422.t1 MTR_3g086100 50.175 285 128 4 5 283 13 289 4.25e-80 260
MS.gene024422.t1 MTR_8g079940 53.008 266 100 5 3 256 23 275 1.50e-78 255
MS.gene024422.t1 MTR_1g013160 82.550 149 25 1 67 214 1 149 1.36e-71 225
MS.gene024422.t1 MTR_3g102590 51.938 258 70 3 2 256 5 211 3.16e-71 227
MS.gene024422.t1 MTR_3g102590 51.464 239 64 2 2 239 5 192 1.35e-64 208
MS.gene024422.t1 MTR_1g032570 52.174 207 73 6 64 255 1 196 1.45e-56 197
MS.gene024422.t1 MTR_3g086100 47.788 226 104 4 64 283 1 218 1.79e-53 188
MS.gene024422.t1 MTR_4g021760 38.996 259 129 5 5 263 11 240 3.54e-53 182
MS.gene024422.t1 MTR_3g102600 68.217 129 41 0 2 130 5 133 8.28e-51 172
MS.gene024422.t1 MTR_3g102600 62.264 53 20 0 189 241 131 183 9.07e-17 79.0
MS.gene024422.t1 MTR_3g102600 67.692 130 42 0 1 130 4 133 2.46e-50 172
MS.gene024422.t1 MTR_3g102600 47.863 117 33 4 189 304 131 220 2.77e-23 98.6
MS.gene024422.t1 MTR_4g021790 38.776 245 123 4 2 246 7 224 3.22e-48 168
MS.gene024422.t1 MTR_4g021855 37.452 259 134 5 2 260 7 237 3.52e-46 163
MS.gene024422.t1 MTR_4g021845 37.815 238 121 4 2 239 7 217 3.73e-46 160
MS.gene024422.t1 MTR_0450s0040 35.968 253 137 3 9 256 8 240 4.06e-45 162
MS.gene024422.t1 MTR_7g026400 37.131 237 126 4 32 260 14 235 2.16e-39 151
MS.gene024422.t1 MTR_4g121020 44.444 207 98 5 84 277 6 208 2.79e-39 150
MS.gene024422.t1 MTR_6g045327 35.656 244 128 5 4 246 13 228 1.08e-36 140
MS.gene024422.t1 MTR_8g105600 34.298 242 150 4 5 245 9 242 1.03e-35 140
MS.gene024422.t1 MTR_3g102600 67.857 84 27 0 47 130 1 84 1.56e-32 121
MS.gene024422.t1 MTR_3g102600 62.264 53 20 0 189 241 82 134 5.51e-17 78.2
MS.gene024422.t1 MTR_3g102600 67.857 84 27 0 47 130 1 84 4.40e-32 121
MS.gene024422.t1 MTR_3g102600 47.863 117 33 4 189 304 82 171 1.75e-23 97.8
MS.gene024422.t1 MTR_5g055260 32.653 245 111 6 2 246 7 197 3.49e-30 118
MS.gene024422.t1 MTR_8g032710 28.516 256 158 7 8 242 20 271 1.80e-26 112
MS.gene024422.t1 MTR_4g064730 55.294 85 36 1 164 246 103 187 6.85e-22 96.7
MS.gene024422.t1 MTR_6g016850 35.433 127 79 2 3 127 7 132 1.42e-21 92.0
MS.gene024422.t1 MTR_3g037390 31.928 166 106 2 9 171 24 185 2.90e-20 94.4
MS.gene024422.t1 MTR_4g023980 30.120 166 90 3 51 216 35 174 6.96e-20 87.4
MS.gene024422.t1 MTR_8g024260 32.738 168 99 3 11 164 38 205 4.15e-19 90.9
MS.gene024422.t1 MTR_7g089010 63.793 58 21 0 189 246 90 147 4.50e-19 88.2
MS.gene024422.t1 MTR_8g093040 35.484 124 80 0 4 127 13 136 4.66e-19 87.8
MS.gene024422.t1 MTR_3g092780 32.374 139 89 2 9 143 56 193 8.58e-19 90.1
MS.gene024422.t1 MTR_3g092780 32.374 139 89 2 9 143 56 193 9.61e-19 90.1
MS.gene024422.t1 MTR_3g092780 34.646 127 78 2 9 131 56 181 1.69e-18 89.0
MS.gene024422.t1 MTR_3g092780 34.646 127 78 2 9 131 56 181 1.77e-18 89.4
MS.gene024422.t1 MTR_7g118260 31.053 190 105 5 2 182 5 177 2.23e-18 88.6
MS.gene024422.t1 MTR_4g108880 29.518 166 110 2 9 171 33 194 1.82e-17 85.9
MS.gene024422.t1 MTR_7g118260 34.307 137 79 3 2 130 5 138 3.63e-17 84.7
MS.gene024422.t1 MTR_1g067110 33.607 122 78 2 9 127 85 206 4.88e-16 81.6
MS.gene024422.t1 MTR_1g067110 33.607 122 78 2 9 127 85 206 5.61e-16 81.3
MS.gene024422.t1 MTR_2g090675 36.364 110 66 1 183 288 154 263 7.51e-16 80.1
MS.gene024422.t1 MTR_6g453220 52.381 84 29 1 207 279 380 463 9.56e-16 80.1
MS.gene024422.t1 MTR_4g061360 35.246 122 76 1 9 127 89 210 1.07e-15 80.5
MS.gene024422.t1 MTR_4g078810 42.500 80 46 0 185 264 153 232 1.32e-15 79.3
MS.gene024422.t1 MTR_5g014040 59.016 61 25 0 188 248 309 369 2.09e-15 79.3
MS.gene024422.t1 MTR_5g014040 34.400 125 76 3 5 127 14 134 1.58e-11 67.0
MS.gene024422.t1 MTR_4g098870 57.377 61 26 0 188 248 328 388 7.21e-15 77.8
MS.gene024422.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 6.55e-11 65.1
MS.gene024422.t1 MTR_4g098870 57.377 61 26 0 188 248 308 368 7.39e-15 77.4
MS.gene024422.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 7.98e-11 64.7
MS.gene024422.t1 MTR_4g098870 57.377 61 26 0 188 248 308 368 7.58e-15 77.4
MS.gene024422.t1 MTR_4g098870 32.258 124 80 2 5 127 14 134 5.79e-11 65.1
MS.gene024422.t1 MTR_4g098870 57.377 61 26 0 188 248 328 388 7.67e-15 77.4
MS.gene024422.t1 MTR_4g098870 32.258 124 80 2 5 127 34 154 9.03e-11 64.7
MS.gene024422.t1 MTR_4g098870 57.377 61 26 0 188 248 347 407 8.65e-15 77.4
MS.gene024422.t1 MTR_4g098870 32.258 124 80 2 5 127 53 173 7.25e-11 65.1
MS.gene024422.t1 MTR_1g093080 45.122 82 44 1 176 256 197 278 5.72e-14 73.9
MS.gene024422.t1 MTR_5g014040 54.545 66 25 1 188 248 269 334 1.62e-13 73.2
MS.gene024422.t1 MTR_5g014040 54.545 66 25 1 188 248 309 374 2.04e-13 73.2
MS.gene024422.t1 MTR_5g014040 34.400 125 76 3 5 127 14 134 1.71e-11 67.0
MS.gene024422.t1 MTR_4g106590 33.588 131 74 2 9 126 26 156 2.45e-13 69.7
MS.gene024422.t1 MTR_7g115530 49.367 79 38 2 188 264 216 294 1.17e-12 68.9
MS.gene024422.t1 MTR_7g115530 49.367 79 38 2 188 264 216 294 1.86e-12 68.9
MS.gene024422.t1 MTR_5g054300 51.562 64 30 1 184 246 192 255 2.18e-12 68.9
MS.gene024422.t1 MTR_5g017980 35.652 115 68 2 187 295 189 303 2.20e-12 68.6
MS.gene024422.t1 MTR_5g017980 35.652 115 68 2 187 295 190 304 2.32e-12 68.6
MS.gene024422.t1 MTR_7g115530 49.367 79 38 2 188 264 216 294 2.62e-12 69.3
MS.gene024422.t1 MTR_6g088950 29.032 124 87 1 7 129 5 128 3.78e-12 66.2
MS.gene024422.t1 MTR_4g086835 48.529 68 33 1 189 254 273 340 3.95e-12 68.6
MS.gene024422.t1 MTR_8g038620 30.435 138 76 3 11 128 11 148 6.08e-12 64.7
MS.gene024422.t1 MTR_7g115530 60.714 56 21 1 188 242 216 271 6.40e-12 67.4
MS.gene024422.t1 MTR_1g080330 56.897 58 24 1 186 242 171 228 6.98e-12 66.6
MS.gene024422.t1 MTR_1g080330 56.897 58 24 1 186 242 171 228 1.10e-11 67.0
MS.gene024422.t1 MTR_2g027800 50.794 63 30 1 186 247 35 97 1.51e-11 65.9
MS.gene024422.t1 MTR_1g053830 46.269 67 33 2 192 255 20 86 2.01e-11 60.8
MS.gene024422.t1 MTR_4g113140 31.724 145 73 2 122 265 192 311 3.48e-11 65.5
MS.gene024422.t1 MTR_5g017980 35.345 116 68 3 187 295 190 305 5.85e-11 64.3
MS.gene024422.t1 MTR_4g086835 47.826 69 33 2 189 254 273 341 7.51e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024422.t1 AT2G25180 57.595 316 113 6 3 305 11 318 1.29e-105 328
MS.gene024422.t1 AT4G31920 51.862 349 123 7 3 328 11 337 1.04e-102 319
MS.gene024422.t1 AT3G16857 43.832 381 150 10 3 346 31 384 5.96e-85 276
MS.gene024422.t1 AT3G16857 43.832 381 150 10 3 346 31 384 1.17e-84 275
MS.gene024422.t1 AT5G58080 49.846 325 125 9 3 308 12 317 3.29e-82 268
MS.gene024422.t1 AT2G01760 47.552 286 141 4 1 278 3 287 1.82e-78 250
MS.gene024422.t1 AT1G67710 52.964 253 109 4 6 252 8 256 5.29e-76 248
MS.gene024422.t1 AT2G01760 43.312 314 141 5 1 278 3 315 2.48e-72 235
MS.gene024422.t1 AT3G16857 42.647 340 131 10 44 346 2 314 1.38e-67 229
MS.gene024422.t1 AT2G01760 45.291 223 113 4 64 278 1 222 5.87e-51 176
MS.gene024422.t1 AT1G67710 51.282 195 85 4 64 252 1 191 2.65e-49 176
MS.gene024422.t1 AT5G49240 37.815 238 142 3 5 239 38 272 9.16e-47 164
MS.gene024422.t1 AT3G62670 37.838 259 135 8 10 258 42 284 1.36e-42 157
MS.gene024422.t1 AT1G49190 31.104 299 153 7 4 276 29 300 2.62e-35 136
MS.gene024422.t1 AT1G67710 45.714 175 85 4 84 252 12 182 3.94e-35 137
MS.gene024422.t1 AT5G07210 29.240 342 190 12 7 312 14 339 8.83e-32 129
MS.gene024422.t1 AT5G07210 28.571 350 190 13 7 312 14 347 2.02e-29 122
MS.gene024422.t1 AT2G27070 29.097 299 158 8 7 266 14 297 4.63e-29 121
MS.gene024422.t1 AT5G59570 50.549 91 45 0 170 260 121 211 5.67e-23 99.4
MS.gene024422.t1 AT5G59570 50.549 91 45 0 170 260 121 211 5.67e-23 99.4
MS.gene024422.t1 AT1G49190 36.800 125 79 0 4 128 29 153 9.92e-22 99.4
MS.gene024422.t1 AT3G46640 38.667 150 82 4 177 319 129 275 2.66e-21 95.1
MS.gene024422.t1 AT3G46640 59.722 72 27 1 177 246 129 200 2.68e-21 95.1
MS.gene024422.t1 AT3G46640 59.722 72 27 1 177 246 129 200 2.68e-21 95.1
MS.gene024422.t1 AT2G46790 37.398 123 72 2 9 127 37 158 8.77e-21 95.1
MS.gene024422.t1 AT2G46790 37.398 123 72 2 9 127 37 158 9.44e-21 95.5
MS.gene024422.t1 AT5G05090 56.164 73 31 1 175 246 65 137 5.19e-20 90.1
MS.gene024422.t1 AT2G40970 63.793 58 21 0 189 246 104 161 9.44e-20 89.0
MS.gene024422.t1 AT3G10760 63.793 58 21 0 189 246 104 161 2.35e-19 89.7
MS.gene024422.t1 AT5G24470 34.375 128 79 2 7 130 48 174 1.38e-18 89.4
MS.gene024422.t1 AT5G61380 30.723 166 108 2 9 171 19 180 1.43e-18 89.4
MS.gene024422.t1 AT5G44190 50.649 77 38 0 182 258 139 215 6.45e-18 85.5
MS.gene024422.t1 AT5G44190 53.521 71 33 0 188 258 145 215 8.30e-18 85.9
MS.gene024422.t1 AT5G60100 35.433 127 78 2 5 127 59 185 6.04e-17 83.2
MS.gene024422.t1 AT5G60100 35.433 127 78 2 5 127 59 185 6.04e-17 83.2
MS.gene024422.t1 AT5G60100 35.433 127 78 2 5 127 59 185 6.04e-17 83.2
MS.gene024422.t1 AT5G60100 35.433 127 78 2 5 127 59 185 1.04e-16 83.2
MS.gene024422.t1 AT5G60100 35.433 127 78 2 5 127 59 185 1.04e-16 83.2
MS.gene024422.t1 AT5G60100 35.433 127 78 2 5 127 59 185 1.04e-16 83.2
MS.gene024422.t1 AT5G60100 35.433 127 78 2 5 127 59 185 1.55e-16 82.8
MS.gene024422.t1 AT5G02810 33.607 122 78 1 9 127 78 199 9.68e-16 80.9
MS.gene024422.t1 AT2G20570 47.945 73 38 0 187 259 150 222 3.25e-15 78.2
MS.gene024422.t1 AT2G20570 50.000 68 34 0 192 259 171 238 1.58e-14 76.3
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 1.84e-14 76.3
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 1.84e-14 76.3
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 1.84e-14 76.3
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 1.84e-14 76.3
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 1.84e-14 76.3
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 2.09e-14 75.9
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 2.09e-14 75.9
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 2.09e-14 75.9
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 2.09e-14 75.9
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 2.09e-14 75.9
MS.gene024422.t1 AT4G18020 38.983 118 57 2 164 281 272 374 2.09e-14 75.9
MS.gene024422.t1 AT3G57040 28.834 163 94 2 11 151 11 173 1.02e-12 68.2
MS.gene024422.t1 AT3G57040 28.834 163 94 2 11 151 11 173 1.02e-12 68.2
MS.gene024422.t1 AT1G25550 39.286 112 54 4 174 277 199 304 2.43e-12 68.6
MS.gene024422.t1 AT1G68670 38.462 104 49 3 164 258 195 292 1.48e-11 66.2
MS.gene024422.t1 AT2G03500 50.820 61 29 1 187 246 230 290 5.89e-11 65.1

Find 100 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCTCTTTACATGGAAAA+AGG 0.209902 7.2:+739821 MS.gene024422:CDS
GGAAGGTAAGAATTTGTTTC+AGG 0.212805 7.2:-739877 None:intergenic
CTTGCTGTTGTTAATCAATT+AGG 0.232624 7.2:+738830 MS.gene024422:CDS
TACCAATCACATGATTATTA+TGG 0.258074 7.2:-739957 None:intergenic
TAGAACTTGTCTCAAGGTTT+TGG 0.268683 7.2:+736905 MS.gene024422:CDS
TGATTTGGCAACATGTAATT+AGG 0.275585 7.2:+738576 MS.gene024422:CDS
CCATTTCCACTATCAAAATT+AGG 0.281786 7.2:-738635 None:intergenic
TATCTGAAAGGAATTAGTTC+TGG 0.286831 7.2:+739544 MS.gene024422:CDS
ATATGATTATATCATTCTTT+TGG 0.303096 7.2:-740692 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.311031 7.2:+736855 MS.gene024422:CDS
ATGAAAAGCCAACTAGAAAA+AGG 0.314880 7.2:+738693 MS.gene024422:CDS
GCCTCATGTGGTTTCTTTGA+AGG 0.325335 7.2:-740030 None:intergenic
CATAAATTATATTACCTGTT+TGG 0.347407 7.2:-740139 None:intergenic
TGAAAGAGGTACAGATGATT+TGG 0.347739 7.2:+738561 MS.gene024422:CDS
CATGTGATTGGTAGTTCAAT+AGG 0.356360 7.2:+739967 MS.gene024422:CDS
AAGGGTGTGTGTAATCCTTT+TGG 0.360628 7.2:+740561 MS.gene024422:CDS
GGCATTCTTTGAACATGGTT+CGG 0.368479 7.2:-739763 None:intergenic
GTTCGGATTGGCGTGAGCTT+TGG 0.373257 7.2:-739746 None:intergenic
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 7.2:+738345 MS.gene024422:CDS
TGAGAGGCTACAGAAGAAAT+TGG 0.392769 7.2:-739784 None:intergenic
GAGAACTGAATCTTGCTTGT+TGG 0.396623 7.2:-739573 None:intergenic
AACCATAATAATCATGTGAT+TGG 0.416988 7.2:+739955 MS.gene024422:CDS
ATTCCTTTCATCACCATCCT+TGG 0.417097 7.2:-738497 None:intergenic
AGAAGAAGCCTCGAGTTGTT+TGG 0.418038 7.2:+738783 MS.gene024422:CDS
CGAGGCTTCTTCTGTGCTGT+TGG 0.425164 7.2:-738773 None:intergenic
TACCATGGTATTGACACCTT+TGG 0.431203 7.2:-736938 None:intergenic
ATCTGAAAGGAATTAGTTCT+GGG 0.433081 7.2:+739545 MS.gene024422:CDS
ACTAGCAATGTGCCATTTCA+AGG 0.436943 7.2:+739922 MS.gene024422:CDS
CTTCGTCAGCCGTAAGTATG+TGG 0.440313 7.2:+740657 MS.gene024422:CDS
GCAAGAACACGCATTCCAAT+AGG 0.445918 7.2:-736870 None:intergenic
TGTACCTCTTTCAATGTGAC+AGG 0.446657 7.2:-738551 None:intergenic
AATGGTCTAAAAGCACTGTT+AGG 0.452029 7.2:-739652 None:intergenic
TAAAGGATAGGTACAACTAC+AGG 0.455880 7.2:+740524 MS.gene024422:intron
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 7.2:+738356 MS.gene024422:CDS
AAACTTGCGGTGTAAGTCAT+TGG 0.458916 7.2:-738807 None:intergenic
TTCAGTTCTCTCACTAGAGT+TGG 0.460910 7.2:+739586 MS.gene024422:CDS
TCTCGTTCAACATTTACAAC+AGG 0.467597 7.2:-740003 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 7.2:-738330 None:intergenic
CAATATGCATGGATTTCAAC+CGG 0.482097 7.2:+739723 MS.gene024422:CDS
AGACCATTTCCATCTGGTGG+TGG 0.485592 7.2:+739667 MS.gene024422:CDS
GGATAAGAATGATGATATTG+AGG 0.489929 7.2:+738718 MS.gene024422:CDS
CCATGCATATTGACACTAGC+AGG 0.493898 7.2:-739712 None:intergenic
GTTGATAGAAGGTGTTGAGA+AGG 0.498197 7.2:+740606 MS.gene024422:CDS
ACGAATAAACCACATACTTA+CGG 0.500506 7.2:-740666 None:intergenic
ATACCACCACCAGATGGAAA+TGG 0.500539 7.2:-739670 None:intergenic
CGATTAGACTCAAGTCCAAA+AGG 0.507846 7.2:-740576 None:intergenic
TGACTTTGATGACGTCTTCG+AGG 0.509927 7.2:-740113 None:intergenic
TAATGTGGCCAGCCATCTCC+AGG 0.513129 7.2:+739239 MS.gene024422:CDS
TTTAGACCATTTCCATCTGG+TGG 0.515382 7.2:+739664 MS.gene024422:CDS
TCTTTGAACATGGTTCGGAT+TGG 0.518790 7.2:-739758 None:intergenic
AGGAGGCCTCCGTCGTTGAT+CGG 0.521097 7.2:+740626 MS.gene024422:CDS
CATCAAAGTCATGACCAAAC+AGG 0.521398 7.2:+740125 MS.gene024422:CDS
GGATTGGCGTGAGCTTTGGC+CGG 0.526522 7.2:-739742 None:intergenic
GTAAGTATGTGGTTTATTCG+TGG 0.528228 7.2:+740668 MS.gene024422:CDS
GATAGAAGGTGTTGAGAAGG+AGG 0.534827 7.2:+740609 MS.gene024422:CDS
GACTTTGATGACGTCTTCGA+GGG 0.537259 7.2:-740112 None:intergenic
ATCGTCTGCGAGTTGATAGA+AGG 0.538494 7.2:+740595 MS.gene024422:CDS
TATGAACATTACTGATTACA+AGG 0.547400 7.2:-738305 None:intergenic
TTGTAAATGTTGAACGAGAA+GGG 0.549447 7.2:+740007 MS.gene024422:CDS
GAAACCTGTCACATTGAAAG+AGG 0.553386 7.2:+738547 MS.gene024422:CDS
CCTGCTAGTGTCAATATGCA+TGG 0.555451 7.2:+739712 MS.gene024422:CDS
CTCATCATATATGCCTCATG+TGG 0.556475 7.2:-740042 None:intergenic
TGAATGTTGAAAATCTTACA+AGG 0.561053 7.2:+739214 MS.gene024422:CDS
GTTGTAAATGTTGAACGAGA+AGG 0.564050 7.2:+740006 MS.gene024422:CDS
GCAATGTGCCATTTCAAGGA+TGG 0.575878 7.2:+739926 MS.gene024422:CDS
ATACTTACGGCTGACGAAGG+CGG 0.583955 7.2:-740653 None:intergenic
GTTCATATGCCAGACATGGA+TGG 0.584384 7.2:+738321 MS.gene024422:CDS
GGCGGATCGCCGATCAACGA+CGG 0.584531 7.2:-740635 None:intergenic
AAATCTTACAAGGGATAATG+TGG 0.585895 7.2:+739224 MS.gene024422:CDS
CACATACTTACGGCTGACGA+AGG 0.586479 7.2:-740656 None:intergenic
AAATTGGCATTCTTTGAACA+TGG 0.589855 7.2:-739768 None:intergenic
GGTTCGTGCACTTTGAGCAG+TGG 0.595278 7.2:-738244 None:intergenic
AAATATAGACTCTATCTGAA+AGG 0.596380 7.2:+739532 MS.gene024422:CDS
TTGCATAACTCACTTATGAC+AGG 0.597051 7.2:-738384 None:intergenic
ACAGGGTGCATATCTTCGCA+AGG 0.597111 7.2:+740542 MS.gene024422:CDS
AAATTCTTACCTTCCACAAG+GGG 0.600441 7.2:+739885 MS.gene024422:CDS
CTACTCATACCACCACCAGA+TGG 0.600621 7.2:-739676 None:intergenic
TAAGAATGATGATATTGAGG+AGG 0.601304 7.2:+738721 MS.gene024422:CDS
GTGATGAAAGGAATTGATCA+TGG 0.607173 7.2:+738506 MS.gene024422:CDS
TGATGATAGAACTTGTCTCA+AGG 0.612252 7.2:+736899 MS.gene024422:CDS
CAAATTCTTACCTTCCACAA+GGG 0.612572 7.2:+739884 MS.gene024422:CDS
TAATGTTCATATGCCAGACA+TGG 0.614385 7.2:+738317 MS.gene024422:CDS
AGAAAACAGTGGATGTTGAG+AGG 0.619813 7.2:-739800 None:intergenic
TTTCTGCAAATGATGATCCA+AGG 0.640841 7.2:+738480 MS.gene024422:CDS
GATCCAAGGATGGTGATGAA+AGG 0.644591 7.2:+738494 MS.gene024422:CDS
ACAAATTCTTACCTTCCACA+AGG 0.649192 7.2:+739883 MS.gene024422:CDS
CTCCAAAGGTGTCAATACCA+TGG 0.650196 7.2:+736936 MS.gene024422:CDS
ATATATGATGAGCAACAAGA+TGG 0.653513 7.2:+740053 MS.gene024422:CDS
TGCAAATGATGATCCAAGGA+TGG 0.655257 7.2:+738484 MS.gene024422:CDS
CAGGGTGCATATCTTCGCAA+GGG 0.657511 7.2:+740543 MS.gene024422:CDS
GAATGTTGAAAATCTTACAA+GGG 0.660347 7.2:+739215 MS.gene024422:CDS
ATAACTCACTTATGACAGGT+AGG 0.663328 7.2:-738380 None:intergenic
AAAGGATAGGTACAACTACA+GGG 0.664550 7.2:+740525 MS.gene024422:intron
TGTAAAGAGCAAGAAAACAG+TGG 0.667428 7.2:-739811 None:intergenic
TCATTGGACCAAACAACTCG+AGG 0.676972 7.2:-738791 None:intergenic
TTCTCTCAACAAGTTCAATG+CGG 0.677431 7.2:-738265 None:intergenic
GGATCGCCGATCAACGACGG+AGG 0.678698 7.2:-740632 None:intergenic
GCCTTCAAAGAAACCACATG+AGG 0.683194 7.2:+740029 MS.gene024422:CDS
TCTGAAAGGAATTAGTTCTG+GGG 0.683638 7.2:+739546 MS.gene024422:CDS
TAACAACAGCAAGAAACTTG+CGG 0.687001 7.2:-738820 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAAATTAATAAATTTCTT+CGG + chr7.2:737749-737768 MS.gene024422:intron 10.0%
!!! TATTAATTTATGATTTTCTT+AGG - chr7.2:737464-737483 None:intergenic 10.0%
!! AAAATAATAAATCTAATCGT+TGG - chr7.2:738119-738138 None:intergenic 15.0%
!!! AGTATTTCAATTTTTTACAT+TGG - chr7.2:737388-737407 None:intergenic 15.0%
!!! ATATCTATTTTTTCTACATT+AGG + chr7.2:737820-737839 MS.gene024422:intron 15.0%
!!! TCTAAAATAATAAATTTGTC+CGG + chr7.2:737350-737369 MS.gene024422:intron 15.0%
!! ATAAAGACGAATATATCAAT+TGG - chr7.2:738917-738936 None:intergenic 20.0%
!! ATGTAATATAATCTAACGTA+TGG + chr7.2:740185-740204 MS.gene024422:intron 20.0%
!! CATAAATTATATTACCTGTT+TGG - chr7.2:740142-740161 None:intergenic 20.0%
!! CTCTATAAATTAATAATGTC+AGG - chr7.2:737776-737795 None:intergenic 20.0%
!! TATCAAAGAAAATTTAAGTC+TGG - chr7.2:737877-737896 None:intergenic 20.0%
!! TATCGTCAAAAATCATATAA+TGG + chr7.2:740231-740250 MS.gene024422:intron 20.0%
!! TATTAGATACAACATAGATT+CGG + chr7.2:737013-737032 MS.gene024422:intron 20.0%
!! TTTAATTGCTTAGAAATTGT+TGG + chr7.2:738059-738078 MS.gene024422:intron 20.0%
!!! AATTTTCTTTGATACTGTTT+AGG + chr7.2:737883-737902 MS.gene024422:intron 20.0%
!!! ATCAATTCAAGAATCTTTTT+TGG - chr7.2:739195-739214 None:intergenic 20.0%
!!! ATTTCATAGATTTTATGTTG+TGG + chr7.2:738148-738167 MS.gene024422:intron 20.0%
!!! GAACTCTTTAATTTAATACT+CGG + chr7.2:737312-737331 MS.gene024422:intron 20.0%
!!! TGCTGCTTAAATATTAAAAA+TGG + chr7.2:736964-736983 MS.gene024422:intron 20.0%
!!! TTTTGTTCTTTAAATCCTTT+TGG + chr7.2:737114-737133 MS.gene024422:intron 20.0%
! AAAAAGGTTCAATGACATTT+GGG + chr7.2:739837-739856 MS.gene024422:CDS 25.0%
! AAATATAGACTCTATCTGAA+AGG + chr7.2:739532-739551 MS.gene024422:CDS 25.0%
! AAATGTATCAGTAACAAGAT+TGG - chr7.2:738414-738433 None:intergenic 25.0%
! AACCATAATAATCATGTGAT+TGG + chr7.2:739955-739974 MS.gene024422:CDS 25.0%
! AATCTGTGAAAGAATTTGAT+CGG - chr7.2:737929-737948 None:intergenic 25.0%
! GAATGTTGAAAATCTTACAA+GGG + chr7.2:739215-739234 MS.gene024422:CDS 25.0%
! GTCAAAATCAATTATTGTCA+CGG - chr7.2:740271-740290 None:intergenic 25.0%
! TACCAATCACATGATTATTA+TGG - chr7.2:739960-739979 None:intergenic 25.0%
! TCAAGTAGAATCAATTATGA+AGG + chr7.2:740403-740422 MS.gene024422:intron 25.0%
! TGAATGTTGAAAATCTTACA+AGG + chr7.2:739214-739233 MS.gene024422:CDS 25.0%
! TTAATTCACGAAAACTTACT+TGG + chr7.2:739476-739495 MS.gene024422:intron 25.0%
! TTAGGACCGAAAATTATAAA+GGG - chr7.2:737446-737465 None:intergenic 25.0%
! TTGAATATGTTTGTTTGAGT+AGG + chr7.2:738951-738970 MS.gene024422:intron 25.0%
!! AATTGATTCATACAAAGCAT+TGG - chr7.2:737851-737870 None:intergenic 25.0%
!! TATGAACATTACTGATTACA+AGG - chr7.2:738308-738327 None:intergenic 25.0%
!! TTTTTATTCCATCCTTGAAA+TGG - chr7.2:739937-739956 None:intergenic 25.0%
!!! GTCAGTTTGTTTGTTTATTT+TGG + chr7.2:737221-737240 MS.gene024422:intron 25.0%
!!! TGTGATCTTATGTTGTTTTT+TGG + chr7.2:738026-738045 MS.gene024422:intron 25.0%
AAATCTTACAAGGGATAATG+TGG + chr7.2:739224-739243 MS.gene024422:CDS 30.0%
AAATTGGCATTCTTTGAACA+TGG - chr7.2:739771-739790 None:intergenic 30.0%
AAGAGATAAAGAAAAACACC+TGG - chr7.2:739260-739279 None:intergenic 30.0%
AATCATATGTGTTTGTGTGT+AGG + chr7.2:737073-737092 MS.gene024422:intron 30.0%
ACGAATAAACCACATACTTA+CGG - chr7.2:740669-740688 None:intergenic 30.0%
ACTGTACATACCAAACAAAA+AGG - chr7.2:737669-737688 None:intergenic 30.0%
ATATATGATGAGCAACAAGA+TGG + chr7.2:740053-740072 MS.gene024422:CDS 30.0%
ATATCAATTGGATTGCTATG+AGG - chr7.2:738905-738924 None:intergenic 30.0%
ATGAAAAGCCAACTAGAAAA+AGG + chr7.2:738693-738712 MS.gene024422:CDS 30.0%
ATGAATGATCAGTTTCCTAT+TGG + chr7.2:736855-736874 MS.gene024422:CDS 30.0%
CCATTTCCACTATCAAAATT+AGG - chr7.2:738638-738657 None:intergenic 30.0%
CTTAGGACCGAAAATTATAA+AGG - chr7.2:737447-737466 None:intergenic 30.0%
GAAAAAGGTTCAATGACATT+TGG + chr7.2:739836-739855 MS.gene024422:CDS 30.0%
GATCAAATTCTTTCACAGAT+TGG + chr7.2:737928-737947 MS.gene024422:intron 30.0%
GGATAAGAATGATGATATTG+AGG + chr7.2:738718-738737 MS.gene024422:CDS 30.0%
TAAGAATGATGATATTGAGG+AGG + chr7.2:738721-738740 MS.gene024422:CDS 30.0%
TACTCTCCTATGATTAATCT+AGG + chr7.2:739055-739074 MS.gene024422:intron 30.0%
TACTGTATCTCCAAAACTTA+AGG - chr7.2:737291-737310 None:intergenic 30.0%
TAGTTGTACCTATCCTTTAA+GGG - chr7.2:740523-740542 None:intergenic 30.0%
TATCGAAAAACTTCATAGCA+CGG - chr7.2:739123-739142 None:intergenic 30.0%
TATCTGAAAGGAATTAGTTC+TGG + chr7.2:739544-739563 MS.gene024422:CDS 30.0%
TTCACTCCTAGATTAATCAT+AGG - chr7.2:739064-739083 None:intergenic 30.0%
TTGTAAATGTTGAACGAGAA+GGG + chr7.2:740007-740026 MS.gene024422:CDS 30.0%
TTTGTGTGAAAATCAATTCC+AGG + chr7.2:740357-740376 MS.gene024422:intron 30.0%
! ATCTGAAAGGAATTAGTTCT+GGG + chr7.2:739545-739564 MS.gene024422:CDS 30.0%
! ATTGATTCTACTTGAAGCTA+TGG - chr7.2:740398-740417 None:intergenic 30.0%
! GGAGATCCCTTTATAATTTT+CGG + chr7.2:737437-737456 MS.gene024422:intron 30.0%
! TCTTTGATACTGTTTAGGTT+CGG + chr7.2:737888-737907 MS.gene024422:intron 30.0%
! TGATTTGGCAACATGTAATT+AGG + chr7.2:738576-738595 MS.gene024422:CDS 30.0%
! TTTGACAATAAGTGAGTTGA+AGG + chr7.2:740285-740304 MS.gene024422:intron 30.0%
!! AAGGTAAAGTGTTTTGTGTT+TGG + chr7.2:740304-740323 MS.gene024422:intron 30.0%
!! CTTGCTGTTGTTAATCAATT+AGG + chr7.2:738830-738849 MS.gene024422:CDS 30.0%
!! GACTAATTTGCCTTTTTGTT+TGG + chr7.2:737656-737675 MS.gene024422:intron 30.0%
!! TAATCATAGGAGAGTATTTG+AGG - chr7.2:739051-739070 None:intergenic 30.0%
!! TTATCCTTCCTTTTTCTAGT+TGG - chr7.2:738704-738723 None:intergenic 30.0%
!!! CCTAATTTTGATAGTGGAAA+TGG + chr7.2:738635-738654 MS.gene024422:CDS 30.0%
!!! TTAAGTTTTGGAGATACAGT+AGG + chr7.2:737290-737309 MS.gene024422:intron 30.0%
!!! TTGTGACACAACTTTTTTGA+CGG + chr7.2:737250-737269 MS.gene024422:intron 30.0%
AAAAGAGTTGCATTACATCC+TGG - chr7.2:738096-738115 None:intergenic 35.0%
AAAGCCAACTAGAAAAAGGA+AGG + chr7.2:738697-738716 MS.gene024422:CDS 35.0%
AAAGGATAGGTACAACTACA+GGG + chr7.2:740525-740544 MS.gene024422:intron 35.0%
AAATTCTTACCTTCCACAAG+GGG + chr7.2:739885-739904 MS.gene024422:CDS 35.0%
ACAAATTCTTACCTTCCACA+AGG + chr7.2:739883-739902 MS.gene024422:CDS 35.0%
ACTACTGTGTTCATATGCAT+TGG - chr7.2:737582-737601 None:intergenic 35.0%
ATAACTCACTTATGACAGGT+AGG - chr7.2:738383-738402 None:intergenic 35.0%
CAAATTCTTACCTTCCACAA+GGG + chr7.2:739884-739903 MS.gene024422:CDS 35.0%
CAATATGCATGGATTTCAAC+CGG + chr7.2:739723-739742 MS.gene024422:CDS 35.0%
CTAATTTACGTTGAGTCTCA+AGG + chr7.2:737604-737623 MS.gene024422:intron 35.0%
CTGTTAAAAATAACCCCTTG+TGG - chr7.2:739901-739920 None:intergenic 35.0%
CTTGCTCTTTACATGGAAAA+AGG + chr7.2:739821-739840 MS.gene024422:CDS 35.0%
GTAAGTATGTGGTTTATTCG+TGG + chr7.2:740668-740687 MS.gene024422:CDS 35.0%
GTACAGTAAACGTAAACAAG+AGG - chr7.2:737538-737557 None:intergenic 35.0%
GTAGTTGTACCTATCCTTTA+AGG - chr7.2:740524-740543 None:intergenic 35.0%
GTGATGAAAGGAATTGATCA+TGG + chr7.2:738506-738525 MS.gene024422:CDS 35.0%
GTTGTAAATGTTGAACGAGA+AGG + chr7.2:740006-740025 MS.gene024422:CDS 35.0%
TAAAAATAACCCCTTGTGGA+AGG - chr7.2:739897-739916 None:intergenic 35.0%
TAAAGAAAAACACCTGGAGA+TGG - chr7.2:739254-739273 None:intergenic 35.0%
TAAAGGATAGGTACAACTAC+AGG + chr7.2:740524-740543 MS.gene024422:intron 35.0%
TAACAACAGCAAGAAACTTG+CGG - chr7.2:738823-738842 None:intergenic 35.0%
TAATGTTCATATGCCAGACA+TGG + chr7.2:738317-738336 MS.gene024422:CDS 35.0%
TACAACATAGATTCGGACTT+CGG + chr7.2:737020-737039 MS.gene024422:intron 35.0%
TCATCATGATTACAATGCCT+AGG - chr7.2:737140-737159 None:intergenic 35.0%
TCTCGTTCAACATTTACAAC+AGG - chr7.2:740006-740025 None:intergenic 35.0%
TGATGATAGAACTTGTCTCA+AGG + chr7.2:736899-736918 MS.gene024422:CDS 35.0%
TGTAAAGAGCAAGAAAACAG+TGG - chr7.2:739814-739833 None:intergenic 35.0%
TTCACAGATTGGACATGATT+TGG + chr7.2:737939-737958 MS.gene024422:intron 35.0%
TTCTCTCAACAAGTTCAATG+CGG - chr7.2:738268-738287 None:intergenic 35.0%
TTGCATAACTCACTTATGAC+AGG - chr7.2:738387-738406 None:intergenic 35.0%
TTGTTGGATTCATTAGATCC+AGG + chr7.2:738075-738094 MS.gene024422:intron 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG + chr7.2:738345-738364 MS.gene024422:CDS 35.0%
TTTCTGCAAATGATGATCCA+AGG + chr7.2:738480-738499 MS.gene024422:CDS 35.0%
! AAAGATAACACCTTCTAGAG+AGG - chr7.2:740484-740503 None:intergenic 35.0%
! AAGATAACACCTTCTAGAGA+GGG - chr7.2:740483-740502 None:intergenic 35.0%
! CATGTGATTGGTAGTTCAAT+AGG + chr7.2:739967-739986 MS.gene024422:CDS 35.0%
! CTGTTTTCTTGCTCTTTACA+TGG + chr7.2:739814-739833 MS.gene024422:CDS 35.0%
! GATGGTGTTTCCTTAAGTTT+TGG + chr7.2:737278-737297 MS.gene024422:intron 35.0%
! GGAAGGTAAGAATTTGTTTC+AGG - chr7.2:739880-739899 None:intergenic 35.0%
! TAGAACTTGTCTCAAGGTTT+TGG + chr7.2:736905-736924 MS.gene024422:CDS 35.0%
! TCTTTAAATCCTTTTGGCCT+AGG + chr7.2:737120-737139 MS.gene024422:intron 35.0%
! TGAAAGAGGTACAGATGATT+TGG + chr7.2:738561-738580 MS.gene024422:CDS 35.0%
! TTTTTACATTGGCCCAAGTT+GGG - chr7.2:737377-737396 None:intergenic 35.0%
!! AATGGTCTAAAAGCACTGTT+AGG - chr7.2:739655-739674 None:intergenic 35.0%
!! GATGTGCCTAATTTTGATAG+TGG + chr7.2:738629-738648 MS.gene024422:CDS 35.0%
!! TCTGAAAGGAATTAGTTCTG+GGG + chr7.2:739546-739565 MS.gene024422:CDS 35.0%
!!! ACTTTTTTGACGGATCTTGA+TGG + chr7.2:737260-737279 MS.gene024422:intron 35.0%
!!! TTTTTTACATTGGCCCAAGT+TGG - chr7.2:737378-737397 None:intergenic 35.0%
AAACTTGCGGTGTAAGTCAT+TGG - chr7.2:738810-738829 None:intergenic 40.0%
AGAAAACAGTGGATGTTGAG+AGG - chr7.2:739803-739822 None:intergenic 40.0%
AGCTTAAATCCATCCATGTC+TGG - chr7.2:738333-738352 None:intergenic 40.0%
ATTACAATGCCTAGGCCAAA+AGG - chr7.2:737132-737151 None:intergenic 40.0%
ATTCCTTTCATCACCATCCT+TGG - chr7.2:738500-738519 None:intergenic 40.0%
ATTTAAGCAGCAGCATACCA+TGG - chr7.2:736956-736975 None:intergenic 40.0%
CAATTTGTACCCTCTCTAGA+AGG + chr7.2:740471-740490 MS.gene024422:intron 40.0%
CATCAAAGTCATGACCAAAC+AGG + chr7.2:740125-740144 MS.gene024422:CDS 40.0%
CATGATCGCAAAACCCTTAA+AGG + chr7.2:740507-740526 MS.gene024422:intron 40.0%
CGATTAGACTCAAGTCCAAA+AGG - chr7.2:740579-740598 None:intergenic 40.0%
CTCATCATATATGCCTCATG+TGG - chr7.2:740045-740064 None:intergenic 40.0%
GAAACCTGTCACATTGAAAG+AGG + chr7.2:738547-738566 MS.gene024422:CDS 40.0%
GAGAACTGAATCTTGCTTGT+TGG - chr7.2:739576-739595 None:intergenic 40.0%
GGCATTCTTTGAACATGGTT+CGG - chr7.2:739766-739785 None:intergenic 40.0%
TCGCAAAACCCTTAAAGGAT+AGG + chr7.2:740512-740531 MS.gene024422:intron 40.0%
TGAGAGGCTACAGAAGAAAT+TGG - chr7.2:739787-739806 None:intergenic 40.0%
TGCAAATGATGATCCAAGGA+TGG + chr7.2:738484-738503 MS.gene024422:CDS 40.0%
TGTACCTCTTTCAATGTGAC+AGG - chr7.2:738554-738573 None:intergenic 40.0%
TTCAGTTCTCTCACTAGAGT+TGG + chr7.2:739586-739605 MS.gene024422:CDS 40.0%
TTTAGACCATTTCCATCTGG+TGG + chr7.2:739664-739683 MS.gene024422:CDS 40.0%
! GCTTTTAGACCATTTCCATC+TGG + chr7.2:739661-739680 MS.gene024422:CDS 40.0%
! TCTTTGAACATGGTTCGGAT+TGG - chr7.2:739761-739780 None:intergenic 40.0%
!! AAGGGTGTGTGTAATCCTTT+TGG + chr7.2:740561-740580 MS.gene024422:CDS 40.0%
!! ACTAGCAATGTGCCATTTCA+AGG + chr7.2:739922-739941 MS.gene024422:CDS 40.0%
!! CATAGGAGAGTATTTGAGGA+AGG - chr7.2:739047-739066 None:intergenic 40.0%
!! GATTCATACAAAGCATTGGC+TGG - chr7.2:737847-737866 None:intergenic 40.0%
!! GTTGATAGAAGGTGTTGAGA+AGG + chr7.2:740606-740625 MS.gene024422:CDS 40.0%
!! TACCATGGTATTGACACCTT+TGG - chr7.2:736941-736960 None:intergenic 40.0%
!! TTTTGGAGAGACTTCTCCAA+AGG + chr7.2:736922-736941 MS.gene024422:CDS 40.0%
AAATTTGTCCGGTCCCAACT+TGG + chr7.2:737361-737380 MS.gene024422:intron 45.0%
AATTTGTCCGGTCCCAACTT+GGG + chr7.2:737362-737381 MS.gene024422:intron 45.0%
ATACCACCACCAGATGGAAA+TGG - chr7.2:739673-739692 None:intergenic 45.0%
CCATGCATATTGACACTAGC+AGG - chr7.2:739715-739734 None:intergenic 45.0%
CCTGCTAGTGTCAATATGCA+TGG + chr7.2:739712-739731 MS.gene024422:CDS 45.0%
GCAAGAACACGCATTCCAAT+AGG - chr7.2:736873-736892 None:intergenic 45.0%
GCACATGCACTTCAGATTGA+AGG - chr7.2:739437-739456 None:intergenic 45.0%
GCCTCATGTGGTTTCTTTGA+AGG - chr7.2:740033-740052 None:intergenic 45.0%
GCCTTCAAAGAAACCACATG+AGG + chr7.2:740029-740048 MS.gene024422:CDS 45.0%
GTTCATATGCCAGACATGGA+TGG + chr7.2:738321-738340 MS.gene024422:CDS 45.0%
TCATTGGACCAAACAACTCG+AGG - chr7.2:738794-738813 None:intergenic 45.0%
! AGAAGAAGCCTCGAGTTGTT+TGG + chr7.2:738783-738802 MS.gene024422:CDS 45.0%
! GATCCAAGGATGGTGATGAA+AGG + chr7.2:738494-738513 MS.gene024422:CDS 45.0%
! TGGTGTGTAGCTTTTGATCC+TGG - chr7.2:740378-740397 None:intergenic 45.0%
!! ATCGTCTGCGAGTTGATAGA+AGG + chr7.2:740595-740614 MS.gene024422:CDS 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG + chr7.2:736936-736955 MS.gene024422:CDS 45.0%
!! GACTTTGATGACGTCTTCGA+GGG - chr7.2:740115-740134 None:intergenic 45.0%
!! GATAGAAGGTGTTGAGAAGG+AGG + chr7.2:740609-740628 MS.gene024422:CDS 45.0%
!! GCAATGTGCCATTTCAAGGA+TGG + chr7.2:739926-739945 MS.gene024422:CDS 45.0%
!! TGACTTTGATGACGTCTTCG+AGG - chr7.2:740116-740135 None:intergenic 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG + chr7.2:738356-738375 MS.gene024422:CDS 45.0%
!!! ATAATAAGATAATAATTTTT+TGG + chr7.2:737415-737434 MS.gene024422:intron 5.0%
!!! TAATAAGATAATAATTTTTT+GGG + chr7.2:737416-737435 MS.gene024422:intron 5.0%
ACAGGGTGCATATCTTCGCA+AGG + chr7.2:740542-740561 MS.gene024422:CDS 50.0%
AGACCATTTCCATCTGGTGG+TGG + chr7.2:739667-739686 MS.gene024422:CDS 50.0%
ATACTTACGGCTGACGAAGG+CGG - chr7.2:740656-740675 None:intergenic 50.0%
CACATACTTACGGCTGACGA+AGG - chr7.2:740659-740678 None:intergenic 50.0%
CAGGGTGCATATCTTCGCAA+GGG + chr7.2:740543-740562 MS.gene024422:CDS 50.0%
CTACTCATACCACCACCAGA+TGG - chr7.2:739679-739698 None:intergenic 50.0%
CTTCGTCAGCCGTAAGTATG+TGG + chr7.2:740657-740676 MS.gene024422:CDS 50.0%
GAAAAACACCTGGAGATGGC+TGG - chr7.2:739250-739269 None:intergenic 50.0%
CGAGGCTTCTTCTGTGCTGT+TGG - chr7.2:738776-738795 None:intergenic 55.0%
GGTTCGTGCACTTTGAGCAG+TGG - chr7.2:738247-738266 None:intergenic 55.0%
TAATGTGGCCAGCCATCTCC+AGG + chr7.2:739239-739258 MS.gene024422:CDS 55.0%
! ACATTGGCCCAAGTTGGGAC+CGG - chr7.2:737372-737391 None:intergenic 55.0%
!! GTTCGGATTGGCGTGAGCTT+TGG - chr7.2:739749-739768 None:intergenic 55.0%
AGGAGGCCTCCGTCGTTGAT+CGG + chr7.2:740626-740645 MS.gene024422:CDS 60.0%
! GGATTGGCGTGAGCTTTGGC+CGG - chr7.2:739745-739764 None:intergenic 60.0%
GGATCGCCGATCAACGACGG+AGG - chr7.2:740635-740654 None:intergenic 65.0%
GGCGGATCGCCGATCAACGA+CGG - chr7.2:740638-740657 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 736855 740708 736855 ID=MS.gene024422
chr7.2 mRNA 736855 740708 736855 ID=MS.gene024422.t1;Parent=MS.gene024422
chr7.2 exon 736855 736957 736855 ID=MS.gene024422.t1.exon1;Parent=MS.gene024422.t1
chr7.2 CDS 736855 736957 736855 ID=cds.MS.gene024422.t1;Parent=MS.gene024422.t1
chr7.2 exon 738241 738393 738241 ID=MS.gene024422.t1.exon2;Parent=MS.gene024422.t1
chr7.2 CDS 738241 738393 738241 ID=cds.MS.gene024422.t1;Parent=MS.gene024422.t1
chr7.2 exon 738477 738860 738477 ID=MS.gene024422.t1.exon3;Parent=MS.gene024422.t1
chr7.2 CDS 738477 738860 738477 ID=cds.MS.gene024422.t1;Parent=MS.gene024422.t1
chr7.2 exon 739184 739260 739184 ID=MS.gene024422.t1.exon4;Parent=MS.gene024422.t1
chr7.2 CDS 739184 739260 739184 ID=cds.MS.gene024422.t1;Parent=MS.gene024422.t1
chr7.2 exon 739532 740146 739532 ID=MS.gene024422.t1.exon5;Parent=MS.gene024422.t1
chr7.2 CDS 739532 740146 739532 ID=cds.MS.gene024422.t1;Parent=MS.gene024422.t1
chr7.2 exon 740529 740708 740529 ID=MS.gene024422.t1.exon6;Parent=MS.gene024422.t1
chr7.2 CDS 740529 740708 740529 ID=cds.MS.gene024422.t1;Parent=MS.gene024422.t1
Gene Sequence

>MS.gene024422

ATGAATGATCAGTTTCCTATTGGAATGCGTGTTCTTGCTGTTGATGATGATAGAACTTGTCTCAAGGTTTTGGAGAGACTTCTCCAAAGGTGTCAATACCATGGTATGCTGCTGCTTAAATATTAAAAATGGATTGTTGACTAAGTAGTGACACAGAATATTAGATACAACATAGATTCGGACTTCGGTTTTAATTTTATAAAATGACATTTGAATGTAATCATATGTGTTTGTGTGTAGGTGCTACTGAAGTTTTTGATTTTGTTCTTTAAATCCTTTTGGCCTAGGCATTGTAATCATGATGATAACATTAATAGTTTCTGTTTTTTTTTTTTTGTTTGTTGTAAACTTGAATTTGTCAGTTGAGTCAGTTTGTTTGTTTATTTTGGTTTTTGTTGTGACACAACTTTTTTGACGGATCTTGATGGTGTTTCCTTAAGTTTTGGAGATACAGTAGGAACTCTTTAATTTAATACTCGGTAAATTAATAAACTCTCTAAAATAATAAATTTGTCCGGTCCCAACTTGGGCCAATGTAAAAAATTGAAATACTCGATAAAATAATAAGATAATAATTTTTTGGGAGATCCCTTTATAATTTTCGGTCCTAAGAAAATCATAAATTAATAATTCATAGAAACTGAAAAAAATATATTAAGAATACATTGAACACATTTGTTCCTCTTGTTTACGTTTACTGTACTTTAAAAGTCATTATTCATTTCCAATGCATATGAACACAGTAGTTACTAATTTACGTTGAGTCTCAAGGTTCACTGCAACGTAATTCTAAGAGACATAGACTAATTTGCCTTTTTGTTTGGTATGTACAGTATAATTACTTAAAAACTCTTTAAATTAATAATTATTAATTTATCGATAAATTAATAACTCTCTAAATTAATAAATTTCTTCGGTCCTGACATTATTAATTTATAGAGTTTTAACTGTATGTGTATTTATGTATATCTATTTTTTCTACATTAGGTCCAGCCAATGCTTTGTATGAATCAATTATGCCAGACTTAAATTTTCTTTGATACTGTTTAGGTTCGGCTAAATTATATTTGACCGATCAAATTCTTTCACAGATTGGACATGATTTGGTTTTTAAAGTTCAAATTTTTAAAAAAATAAATAAAAATAAATAAAAATTGTGTGTCTAATCTTTTGTGATCTTATGTTGTTTTTTGGATTTCAAAGATTTAATTGCTTAGAAATTGTTGGATTCATTAGATCCAGGATGTAATGCAACTCTTTTCCAACGATTAGATTTATTATTTTTAAAGTAACATTTCATAGATTTTATGTTGTGGTTGTATCTGCAATGTATCATTTTTACTCTTTCAAAATGATATTAATTGTAGTTATGATTATCTGTTTCAGTAACCACTGCTCAAAGTGCACGAACCGCATTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTTGTAATCAGTAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTGAGTTATGCAATTCTCCAATCTTGTTACTGATACATTTATAACAAATTATAGTGTTTTGATTACAAAGTTTTTGATTTCAGTGTTTTCTGCAAATGATGATCCAAGGATGGTGATGAAAGGAATTGATCATGGTGCTTGTGATTATCTGCTGAAACCTGTCACATTGAAAGAGGTACAGATGATTTGGCAACATGTAATTAGGAAGAAGAAAACTAGCAAAAGAAGCAATCATGATGTGCCTAATTTTGATAGTGGAAATGGAATAGATTCAGCTGTGACAAGAAATTCTGATCAAAATGAAAAGCCAACTAGAAAAAGGAAGGATAAGAATGATGATATTGAGGAGGAGAATGAAGATGATCATGACAATGATGATCCAACAGCACAGAAGAAGCCTCGAGTTGTTTGGTCCAATGACTTACACCGCAAGTTTCTTGCTGTTGTTAATCAATTAGGCATTGAAAGTACGTTCTTTCGTCTGTCTTCAATGCGCAAAAATAATTGTCCTCATAGCAATCCAATTGATATATTCGTCTTTATTTTTTCTCATTTTATTGAATATGTTTGTTTGAGTAGGTTATAGCAATTCAATGTTGAGATTAATATTATAATATGAAAATGAAAATTTTACTTTGTATTATTTGAAACCTTCCTCAAATACTCTCCTATGATTAATCTAGGAGTGAATGTGAATCTCATGTCATGATCATTACTCATGTTTAACCGTGCTATGAAGTTTTTCGATACTAAACTGTGTTTCTTATTTTAGTTGTTATTTTGCTTGCAGATGCTATACCAAAAAAGATTCTTGAATTGATGAATGTTGAAAATCTTACAAGGGATAATGTGGCCAGCCATCTCCAGGTGTTTTTCTTTATCTCTTATGCTTTACTTTTATACGCTATGAAGACAACAATGACATTAACGTAGATGCTAAACATGACACTGACATAGTGACACTGCTAATAATTTACAAATTTGAAGTAATTAAATTAAATGTAATCACACGTATCAGCGTCTGACACTAGACAAACACACCTTCAATCTGAAGTGCATGTGCTACGAAGTTCTCAAGCACTTTAATTCACGAAAACTTACTTGGTTCTTTTTTTTTTTTTTTTTTTTAAATCTGCAGAAATATAGACTCTATCTGAAAGGAATTAGTTCTGGGGAAAACCAACAAGCAAGATTCAGTTCTCTCACTAGAGTTGGAGAACATTTTCATACATTGAATAACTCTAGACAGTTTCATAATCCTAACAGTGCTTTTAGACCATTTCCATCTGGTGGTGGTATGAGTAGCAGATTGAACACTCCTGCTAGTGTCAATATGCATGGATTTCAACCGGCCAAAGCTCACGCCAATCCGAACCATGTTCAAAGAATGCCAATTTCTTCTGTAGCCTCTCAACATCCACTGTTTTCTTGCTCTTTACATGGAAAAAGGTTCAATGACATTTGGGCAAGCACTACGCAATTACCTGAAACAAATTCTTACCTTCCACAAGGGGTTATTTTTAACAGCACTAGCAATGTGCCATTTCAAGGATGGAATAAAAACCATAATAATCATGTGATTGGTAGTTCAATAGGCTCTTCTACAACTCCTGTTGTAAATGTTGAACGAGAAGGGCCTTCAAAGAAACCACATGAGGCATATATGATGAGCAACAAGATGGAGTCTAAAAGTTGCTGTATCTCTAATGATATTTGTTCCCTCGAAGACGTCATCAAAGTCATGACCAAACAGGTAATATAATTTATGCAATGTGATGAAGTTCTTATGCAATGTAATATAATCTAACGTATGGTTGTTGCTTTGAGCATAATTAATTATCGTCAAAAATCATATAATGGCTTAAACATGTCTGCCGTGACAATAATTGATTTTGACAATAAGTGAGTTGAAGGTAAAGTGTTTTGTGTTTGGAACATATGTAAAAATGAGTTGAACTATAAATTTGTGTGAAAATCAATTCCAGGATCAAAAGCTACACACCATAGCTTCAAGTAGAATCAATTATGAAGGTAGAATCAATTCTTATTCTACTTTAAAAAAAAATAATGTCAAAATCAATTTGTACCCTCTCTAGAAGGTGTTATCTTTGCGCATGATCGCAAAACCCTTAAAGGATAGGTACAACTACAGGGTGCATATCTTCGCAAGGGTGTGTGTAATCCTTTTGGACTTGAGTCTAATCGTCTGCGAGTTGATAGAAGGTGTTGAGAAGGAGGCCTCCGTCGTTGATCGGCGATCCGCCTTCGTCAGCCGTAAGTATGTGGTTTATTCGTGGACCAAAAGAATGATATAA
Protein sequence

>MS.gene024422.t1

MNDQFPIGMRVLAVDDDRTCLKVLERLLQRCQYHVTTAQSARTALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPRMVMKGIDHGACDYLLKPVTLKEVQMIWQHVIRKKKTSKRSNHDVPNFDSGNGIDSAVTRNSDQNEKPTRKRKDKNDDIEEENEDDHDNDDPTAQKKPRVVWSNDLHRKFLAVVNQLGIENAIPKKILELMNVENLTRDNVASHLQKYRLYLKGISSGENQQARFSSLTRVGEHFHTLNNSRQFHNPNSAFRPFPSGGGMSSRLNTPASVNMHGFQPAKAHANPNHVQRMPISSVASQHPLFSCSLHGKRFNDIWASTTQLPETNSYLPQGVIFNSTSNVPFQGWNKNHNNHVIGSSIGSSTTPVVNVEREGPSKKPHEAYMMSNKMESKSCCISNDICSLEDVIKVMTKQDRYNYRVHIFARVCVILLDLSLIVCELIEGVEKEASVVDRRSAFVSRKYVVYSWTKRMI