AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024451

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024451.t1 MTR_7g117200 91.343 335 20 2 1 333 1 328 0.0 618
MS.gene024451.t1 MTR_8g027860 44.565 368 142 15 1 329 1 345 5.26e-78 242
MS.gene024451.t1 MTR_8g099350 36.585 369 189 12 1 332 1 361 2.04e-55 184
MS.gene024451.t1 MTR_3g090860 53.125 192 67 9 12 185 7 193 1.87e-53 179
MS.gene024451.t1 MTR_7g073430 33.500 200 108 5 9 198 11 195 5.47e-26 105
MS.gene024451.t1 MTR_7g073380 32.275 189 97 5 9 184 12 182 4.78e-25 102
MS.gene024451.t1 MTR_8g005750 36.364 176 85 5 15 184 8 162 1.97e-24 100
MS.gene024451.t1 MTR_5g067680 40.650 123 59 1 118 240 195 303 4.71e-21 92.8
MS.gene024451.t1 MTR_2g075690 30.622 209 109 6 11 195 15 211 9.27e-21 91.3
MS.gene024451.t1 MTR_0002s1250 30.556 180 96 7 15 184 10 170 2.46e-20 89.4
MS.gene024451.t1 MTR_5g067700 31.892 185 101 6 7 189 10 171 5.82e-20 87.8
MS.gene024451.t1 MTR_3g093830 30.570 193 103 5 18 196 23 198 2.48e-19 87.4
MS.gene024451.t1 MTR_2g075700 45.349 86 37 2 100 184 127 203 8.21e-19 85.9
MS.gene024451.t1 MTR_2g075680 45.349 86 38 2 99 184 131 207 1.36e-17 82.4
MS.gene024451.t1 MTR_2g075750 49.180 61 31 0 124 184 27 87 7.09e-17 77.8
MS.gene024451.t1 MTR_2g075610 49.180 61 31 0 124 184 28 88 9.43e-17 77.8
MS.gene024451.t1 MTR_1g009613 43.529 85 44 2 98 182 237 317 3.72e-16 78.6
MS.gene024451.t1 MTR_7g109600 40.870 115 63 3 78 189 3 115 5.47e-16 77.0
MS.gene024451.t1 MTR_7g028415 55.224 67 25 2 124 190 92 153 1.51e-15 73.9
MS.gene024451.t1 MTR_7g028710 55.224 67 25 2 124 190 92 153 1.51e-15 73.9
MS.gene024451.t1 MTR_7g109800 40.594 101 56 2 88 187 107 204 1.80e-15 76.3
MS.gene024451.t1 MTR_8g028565 38.679 106 59 3 82 187 114 213 4.20e-15 75.5
MS.gene024451.t1 MTR_4g127040 32.624 141 72 3 42 182 183 300 4.38e-15 75.1
MS.gene024451.t1 MTR_8g098945 47.297 74 33 3 121 194 125 192 6.10e-15 74.3
MS.gene024451.t1 MTR_8g087000 39.796 98 52 2 118 215 70 160 1.01e-14 73.2
MS.gene024451.t1 MTR_8g087000 36.885 122 65 3 99 215 45 159 1.04e-14 73.2
MS.gene024451.t1 MTR_8g032510 50.000 60 30 0 125 184 119 178 1.08e-14 73.6
MS.gene024451.t1 MTR_7g079040 51.515 66 26 2 126 191 155 214 3.89e-14 72.4
MS.gene024451.t1 MTR_5g016610 54.386 57 24 1 126 182 200 254 4.53e-14 71.6
MS.gene024451.t1 MTR_2g032030 41.748 103 52 4 126 222 268 368 4.97e-14 72.8
MS.gene024451.t1 MTR_2g032030 41.748 103 52 4 126 222 268 368 5.89e-14 72.8
MS.gene024451.t1 MTR_7g079010 45.122 82 40 2 124 205 140 216 6.41e-14 70.5
MS.gene024451.t1 MTR_2g083870 43.750 96 48 3 99 190 50 143 8.18e-14 70.9
MS.gene024451.t1 MTR_5g043880 52.381 63 26 2 124 186 123 181 9.95e-14 70.9
MS.gene024451.t1 MTR_2g016770 46.479 71 32 2 126 196 36 100 1.13e-13 69.7
MS.gene024451.t1 MTR_8g096730 46.269 67 31 2 124 190 69 130 1.15e-13 68.2
MS.gene024451.t1 MTR_2g016770 46.479 71 32 2 126 196 28 92 1.26e-13 69.3
MS.gene024451.t1 MTR_1g070455 52.632 57 25 1 126 182 294 348 2.68e-13 70.5
MS.gene024451.t1 MTR_4g094908 50.877 57 26 1 126 182 267 321 6.98e-13 68.9
MS.gene024451.t1 MTR_4g082580 44.118 68 35 1 125 192 145 209 7.31e-13 69.3
MS.gene024451.t1 MTR_4g082580 41.772 79 43 1 112 190 265 340 2.35e-11 64.7
MS.gene024451.t1 MTR_1g086790 50.000 64 27 2 124 187 157 215 7.46e-13 68.9
MS.gene024451.t1 MTR_3g009470 45.714 70 32 3 125 194 280 343 7.77e-13 69.7
MS.gene024451.t1 MTR_3g009470 33.663 101 59 2 124 219 494 591 6.89e-11 63.9
MS.gene024451.t1 MTR_5g091390 49.153 59 27 1 124 182 156 211 7.84e-13 68.6
MS.gene024451.t1 MTR_4g094588 47.143 70 32 2 124 193 177 241 7.95e-13 68.9
MS.gene024451.t1 MTR_3g009470 45.714 70 32 3 125 194 280 343 8.27e-13 69.3
MS.gene024451.t1 MTR_3g095080 44.444 72 37 1 112 183 131 199 1.28e-12 66.6
MS.gene024451.t1 MTR_3g095080 44.444 72 37 1 112 183 155 223 1.29e-12 67.0
MS.gene024451.t1 MTR_2g033820 48.438 64 30 1 124 187 202 262 1.37e-12 68.2
MS.gene024451.t1 MTR_8g092140 50.000 60 27 1 124 183 159 215 1.56e-12 66.6
MS.gene024451.t1 MTR_7g011820 46.875 64 30 2 125 188 228 287 1.69e-12 68.6
MS.gene024451.t1 MTR_7g011820 44.776 67 34 1 124 190 409 472 4.40e-12 67.4
MS.gene024451.t1 MTR_7g100510 31.667 120 78 2 126 245 190 305 1.79e-12 68.2
MS.gene024451.t1 MTR_5g042920 46.269 67 33 1 124 190 319 382 1.81e-12 68.2
MS.gene024451.t1 MTR_5g042920 35.652 115 65 4 125 239 151 256 5.83e-11 63.5
MS.gene024451.t1 MTR_7g062220 49.275 69 28 2 114 182 117 178 2.56e-12 67.0
MS.gene024451.t1 MTR_1g099600 44.928 69 32 2 126 194 186 248 2.64e-12 67.0
MS.gene024451.t1 MTR_5g094430 44.776 67 34 1 124 190 363 426 3.01e-12 67.8
MS.gene024451.t1 MTR_5g094430 45.833 72 33 3 123 194 198 263 5.00e-12 67.0
MS.gene024451.t1 MTR_5g073620 43.284 67 38 0 125 191 672 738 3.44e-12 67.8
MS.gene024451.t1 MTR_3g056100 47.143 70 31 3 125 194 243 306 3.82e-12 67.4
MS.gene024451.t1 MTR_3g056100 44.776 67 34 1 124 190 401 464 6.52e-12 66.6
MS.gene024451.t1 MTR_3g056100 47.143 70 31 3 125 194 243 306 3.97e-12 67.4
MS.gene024451.t1 MTR_3g056100 44.776 67 34 1 124 190 414 477 7.10e-12 66.6
MS.gene024451.t1 MTR_3g108960 52.632 57 25 1 126 182 315 369 4.15e-12 67.0
MS.gene024451.t1 MTR_5g094430 44.776 67 34 1 124 190 251 314 4.22e-12 67.0
MS.gene024451.t1 MTR_5g094430 45.833 72 33 3 123 194 86 151 5.75e-12 66.6
MS.gene024451.t1 MTR_5g094430 44.776 67 34 1 124 190 229 292 4.33e-12 67.0
MS.gene024451.t1 MTR_5g094430 45.833 72 33 3 123 194 64 129 6.29e-12 66.6
MS.gene024451.t1 MTR_3g056100 47.143 70 31 3 125 194 99 162 4.76e-12 67.0
MS.gene024451.t1 MTR_3g056100 44.776 67 34 1 124 190 257 320 8.75e-12 66.2
MS.gene024451.t1 MTR_3g056100 47.143 70 31 3 125 194 99 162 4.80e-12 67.0
MS.gene024451.t1 MTR_3g056100 44.776 67 34 1 124 190 270 333 9.15e-12 66.2
MS.gene024451.t1 MTR_8g007270 45.070 71 36 1 112 182 141 208 5.42e-12 65.1
MS.gene024451.t1 MTR_0117s0040 52.632 57 25 1 126 182 75 129 6.11e-12 65.1
MS.gene024451.t1 MTR_7g080020 44.615 65 32 2 125 189 280 340 1.09e-11 66.2
MS.gene024451.t1 MTR_4g122530 45.312 64 32 1 124 187 207 267 1.15e-11 65.9
MS.gene024451.t1 MTR_2g088000 48.485 66 32 1 124 189 348 411 4.01e-11 64.3
MS.gene024451.t1 MTR_5g074400 44.776 67 34 1 124 190 419 482 4.08e-11 64.3
MS.gene024451.t1 MTR_1g015140 38.824 85 45 3 107 189 82 161 8.38e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024451.t1 AT4G11070 38.728 346 147 13 3 324 2 306 3.20e-51 172
MS.gene024451.t1 AT4G11070 37.423 326 147 11 3 324 2 274 1.12e-46 159
MS.gene024451.t1 AT4G23810 42.478 226 85 7 11 195 13 234 8.93e-43 150
MS.gene024451.t1 AT5G24110 37.066 259 120 10 12 265 17 237 9.03e-43 150
MS.gene024451.t1 AT2G46400 37.542 301 153 10 12 309 8 276 8.90e-41 144
MS.gene024451.t1 AT3G56400 32.487 197 99 5 3 184 2 179 2.07e-26 106
MS.gene024451.t1 AT2G40750 27.574 272 161 7 5 244 8 275 2.66e-23 99.0
MS.gene024451.t1 AT2G40740 42.202 109 61 1 99 205 145 253 2.85e-22 95.1
MS.gene024451.t1 AT2G40740 42.202 109 61 1 99 205 154 262 3.67e-22 95.1
MS.gene024451.t1 AT5G45260 47.423 97 41 4 99 195 1188 1274 8.11e-19 88.2
MS.gene024451.t1 AT1G66550 29.282 181 90 6 16 184 13 167 5.62e-16 77.0
MS.gene024451.t1 AT1G66600 40.909 88 45 1 104 184 75 162 2.69e-15 74.7
MS.gene024451.t1 AT5G52830 54.839 62 26 1 126 187 166 225 2.98e-15 76.3
MS.gene024451.t1 AT1G29280 53.226 62 25 2 126 187 75 132 6.35e-15 73.9
MS.gene024451.t1 AT1G66550 38.636 88 47 1 104 184 75 162 1.35e-14 72.8
MS.gene024451.t1 AT4G31550 44.048 84 43 2 99 182 221 300 1.98e-14 73.6
MS.gene024451.t1 AT5G49520 50.000 64 29 1 124 187 220 280 2.18e-14 73.9
MS.gene024451.t1 AT4G31550 54.386 57 24 1 126 182 247 301 5.09e-14 72.4
MS.gene024451.t1 AT4G01250 51.613 62 26 2 126 187 129 186 8.79e-14 71.2
MS.gene024451.t1 AT2G30590 50.794 63 27 2 126 188 314 372 9.53e-14 72.0
MS.gene024451.t1 AT1G30650 41.905 105 48 6 95 194 184 280 9.92e-14 72.0
MS.gene024451.t1 AT2G34830 35.938 128 58 6 78 187 152 273 1.43e-13 71.6
MS.gene024451.t1 AT1G66560 46.667 60 32 0 125 184 103 162 1.47e-13 70.1
MS.gene024451.t1 AT2G34830 35.938 128 58 6 78 187 161 282 1.63e-13 71.2
MS.gene024451.t1 AT1G69310 40.476 84 47 1 99 182 121 201 4.01e-13 69.3
MS.gene024451.t1 AT1G69310 40.476 84 47 1 99 182 121 201 4.01e-13 69.3
MS.gene024451.t1 AT1G69310 40.476 84 47 1 99 182 121 201 4.01e-13 69.3
MS.gene024451.t1 AT2G24570 54.386 57 24 1 126 182 244 298 4.09e-13 69.7
MS.gene024451.t1 AT2G37260 35.345 116 60 3 72 182 218 323 5.17e-13 69.3
MS.gene024451.t1 AT2G37260 35.345 116 60 3 72 182 216 321 5.67e-13 69.3
MS.gene024451.t1 AT2G37260 35.345 116 60 3 72 182 298 403 6.31e-13 69.7
MS.gene024451.t1 AT5G13080 51.562 64 26 2 124 187 66 124 8.09e-13 65.5
MS.gene024451.t1 AT5G41570 43.038 79 40 2 112 190 85 158 8.55e-13 66.2
MS.gene024451.t1 AT3G58710 43.011 93 45 4 99 187 40 128 9.24e-13 67.8
MS.gene024451.t1 AT1G29860 40.476 84 45 2 110 193 121 199 1.15e-12 67.8
MS.gene024451.t1 AT4G24240 41.667 84 45 2 99 182 257 336 1.20e-12 68.6
MS.gene024451.t1 AT1G64000 40.659 91 43 3 112 196 101 186 1.27e-12 66.2
MS.gene024451.t1 AT2G23320 50.877 57 26 1 126 182 241 295 1.31e-12 68.2
MS.gene024451.t1 AT3G58710 50.000 62 27 2 126 187 72 129 1.34e-12 67.4
MS.gene024451.t1 AT4G18170 35.238 105 63 2 83 187 130 229 1.53e-12 67.8
MS.gene024451.t1 AT2G47260 37.778 90 53 1 98 187 147 233 1.63e-12 67.8
MS.gene024451.t1 AT2G46130 48.438 64 28 2 124 187 29 87 1.63e-12 63.5
MS.gene024451.t1 AT5G28650 52.632 57 25 1 126 182 263 317 1.83e-12 67.8
MS.gene024451.t1 AT4G23550 46.774 62 31 1 121 182 136 195 1.86e-12 67.4
MS.gene024451.t1 AT1G80590 39.241 79 45 1 112 187 69 147 2.88e-12 65.9
MS.gene024451.t1 AT2G44745 45.070 71 36 1 112 182 132 199 4.29e-12 65.1
MS.gene024451.t1 AT5G01900 43.750 64 36 0 126 189 111 174 5.29e-12 65.5
MS.gene024451.t1 AT3G01970 48.529 68 30 2 124 191 64 126 6.05e-12 63.2
MS.gene024451.t1 AT4G26640 45.714 70 32 3 125 194 171 234 6.12e-12 67.0
MS.gene024451.t1 AT4G26640 44.776 67 34 1 124 190 340 403 9.21e-12 66.2
MS.gene024451.t1 AT4G26640 45.714 70 32 3 125 194 211 274 6.78e-12 66.6
MS.gene024451.t1 AT4G26640 44.776 67 34 1 124 190 380 443 1.04e-11 66.2
MS.gene024451.t1 AT4G26640 45.070 71 33 3 124 194 138 202 6.95e-12 66.6
MS.gene024451.t1 AT4G26640 44.776 67 34 1 124 190 308 371 1.11e-11 65.9
MS.gene024451.t1 AT4G26640 45.070 71 33 3 124 194 34 98 7.27e-12 66.2
MS.gene024451.t1 AT4G26640 44.776 67 34 1 124 190 204 267 1.21e-11 65.5
MS.gene024451.t1 AT4G26640 45.070 71 33 3 124 194 34 98 7.27e-12 66.2
MS.gene024451.t1 AT4G26640 44.776 67 34 1 124 190 204 267 1.21e-11 65.5
MS.gene024451.t1 AT4G26640 45.070 71 33 3 124 194 34 98 7.27e-12 66.2
MS.gene024451.t1 AT4G26640 44.776 67 34 1 124 190 204 267 1.21e-11 65.5
MS.gene024451.t1 AT4G26640 45.070 71 33 3 124 194 34 98 7.27e-12 66.2
MS.gene024451.t1 AT4G26640 44.776 67 34 1 124 190 204 267 1.21e-11 65.5
MS.gene024451.t1 AT3G01080 48.438 64 29 2 125 188 205 264 7.36e-12 65.9
MS.gene024451.t1 AT5G46350 46.875 64 29 2 124 187 182 240 7.54e-12 65.9
MS.gene024451.t1 AT3G62340 48.438 64 30 1 124 187 117 177 8.62e-12 65.1
MS.gene024451.t1 AT3G04670 50.877 57 26 1 126 182 263 317 8.79e-12 65.5
MS.gene024451.t1 AT4G26440 50.794 63 27 2 125 187 178 236 9.33e-12 66.2
MS.gene024451.t1 AT4G26440 46.269 67 33 1 124 190 371 434 2.09e-11 65.1
MS.gene024451.t1 AT1G13960 46.875 64 30 2 125 188 229 288 9.51e-12 66.2
MS.gene024451.t1 AT1G13960 47.458 59 28 1 124 182 408 463 1.29e-11 65.9
MS.gene024451.t1 AT3G01080 46.970 66 31 2 125 190 205 266 1.16e-11 65.9
MS.gene024451.t1 AT2G25000 44.776 67 35 1 123 189 123 187 1.21e-11 64.3
MS.gene024451.t1 AT2G25000 44.776 67 35 1 123 189 101 165 1.24e-11 63.9
MS.gene024451.t1 AT2G25000 44.776 67 35 1 123 189 101 165 1.24e-11 63.9
MS.gene024451.t1 AT2G03340 45.455 66 32 2 125 190 250 311 1.27e-11 65.9
MS.gene024451.t1 AT2G03340 43.284 67 35 1 124 190 414 477 2.64e-11 64.7
MS.gene024451.t1 AT2G25000 44.776 67 35 1 123 189 144 208 1.42e-11 64.3
MS.gene024451.t1 AT3G01080 46.970 66 31 2 125 190 167 228 1.43e-11 65.5
MS.gene024451.t1 AT2G04880 44.304 79 36 4 119 197 105 175 1.79e-11 65.5
MS.gene024451.t1 AT2G04880 40.299 67 37 1 124 190 306 369 8.41e-11 63.2
MS.gene024451.t1 AT1G13960 46.875 64 30 2 125 188 76 135 1.80e-11 65.1
MS.gene024451.t1 AT1G13960 47.458 59 28 1 124 182 255 310 1.83e-11 65.1
MS.gene024451.t1 AT2G04880 44.304 79 36 4 119 197 105 175 1.80e-11 65.5
MS.gene024451.t1 AT2G04880 40.299 67 37 1 124 190 282 345 6.46e-11 63.5
MS.gene024451.t1 AT4G39410 41.176 85 46 2 98 182 197 277 3.26e-11 63.9
MS.gene024451.t1 AT4G01720 37.963 108 60 4 81 187 196 297 3.38e-11 64.3
MS.gene024451.t1 AT5G45050 42.500 80 40 3 108 187 1026 1099 4.89e-11 64.3
MS.gene024451.t1 AT5G45050 42.500 80 40 3 108 187 1165 1238 4.95e-11 64.3
MS.gene024451.t1 AT5G45050 42.500 80 40 3 108 187 1137 1210 5.06e-11 64.3
MS.gene024451.t1 AT5G07100 47.458 59 28 1 124 182 233 288 5.36e-11 63.2
MS.gene024451.t1 AT5G07100 42.857 70 35 2 125 194 117 181 8.36e-11 62.4
MS.gene024451.t1 AT5G07100 47.458 59 28 1 124 182 140 195 6.60e-11 61.6
MS.gene024451.t1 AT5G07100 42.857 70 35 2 125 194 24 88 8.54e-11 61.2
MS.gene024451.t1 AT5G07100 47.458 59 28 1 124 182 140 195 6.60e-11 61.6
MS.gene024451.t1 AT5G07100 42.857 70 35 2 125 194 24 88 8.54e-11 61.2
MS.gene024451.t1 AT5G07100 47.458 59 28 1 124 182 140 195 6.60e-11 61.6
MS.gene024451.t1 AT5G07100 42.857 70 35 2 125 194 24 88 8.54e-11 61.2
MS.gene024451.t1 AT5G07100 47.458 59 28 1 124 182 140 195 6.60e-11 61.6
MS.gene024451.t1 AT5G07100 42.857 70 35 2 125 194 24 88 8.54e-11 61.2
MS.gene024451.t1 AT2G40740 45.902 61 33 0 145 205 149 209 7.41e-11 62.0
MS.gene024451.t1 AT2G21900 40.299 67 37 1 123 189 107 170 7.47e-11 61.2
MS.gene024451.t1 AT5G43290 40.541 111 50 5 120 224 109 209 8.93e-11 62.0
MS.gene024451.t1 AT5G22570 44.068 59 33 0 126 184 111 169 9.53e-11 62.4

Find 70 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTATGATGACCTCACTTT+TGG 0.188414 7.2:-997992 None:intergenic
TCAACATAGTAAGTGCTTTC+TGG 0.207919 7.2:-997894 None:intergenic
TCCCTCAAAGTTAAAGATAA+AGG 0.227041 7.2:+999172 MS.gene024451:CDS
CTCAAGCTAAGCCTTTAAAA+AGG 0.231958 7.2:+999144 MS.gene024451:CDS
ATTCTGAAGTTGGTCCATTT+TGG 0.232813 7.2:-999430 None:intergenic
CAAGAATTCATTGGTTTCAT+TGG 0.239108 7.2:-997848 None:intergenic
TTCACTTGTTCCTTCCATTT+AGG 0.269402 7.2:-998347 None:intergenic
CAATTCACCAATCCTTGATT+TGG 0.271018 7.2:+999493 MS.gene024451:CDS
CATTGGATGATATAATTATA+TGG 0.275590 7.2:-997831 None:intergenic
AGGATTATCTGAAGGGTCTT+TGG 0.285031 7.2:+998385 MS.gene024451:CDS
CCGAGGGAAAATACCCTCTT+TGG 0.291656 7.2:-999306 None:intergenic
CTTTCCTTGGATTAATTCAT+TGG 0.302740 7.2:-997785 None:intergenic
CAAATCAAGGATTGGTGAAT+TGG 0.310097 7.2:-999492 None:intergenic
CCCTCAAAGTTAAAGATAAA+GGG 0.333691 7.2:+999173 MS.gene024451:CDS
CCCTTTATCTTTAACTTTGA+GGG 0.337711 7.2:-999173 None:intergenic
GCAGAAAGAGCATGCCTAAA+TGG 0.338464 7.2:+998333 MS.gene024451:intron
TATGGTCAAAAGGATATTCT+TGG 0.360560 7.2:+998431 MS.gene024451:CDS
GAATATAGCTGGAGAAAATA+TGG 0.367378 7.2:+998413 MS.gene024451:CDS
GCCCTTTATCTTTAACTTTG+AGG 0.367772 7.2:-999174 None:intergenic
TACAATGGATTATGCCAAAA+TGG 0.369767 7.2:+999416 MS.gene024451:CDS
TTTGAACCTGCTTTGTTGCT+AGG 0.370195 7.2:-999058 None:intergenic
TAAGTTACCTCAATTATTGT+TGG 0.376442 7.2:-999095 None:intergenic
TTTGTCAATCAAGAATTCAT+TGG 0.407531 7.2:-997857 None:intergenic
GCTTTGCTAACTCCTTTCCT+TGG 0.408135 7.2:-997798 None:intergenic
GGACTCTCGTTTGTAAAAGA+AGG 0.431784 7.2:-997970 None:intergenic
TGGTTTGTCAGAATATCAAT+TGG 0.434536 7.2:+999391 MS.gene024451:CDS
GTGATATCAGATTCTGAAGT+TGG 0.434696 7.2:-999440 None:intergenic
TACAGATGCACACATAGAAA+TGG 0.436912 7.2:+999026 MS.gene024451:CDS
GAAGCAAGATATCCAAATCA+AGG 0.449238 7.2:-999505 None:intergenic
TTTAACATCAAGTTCTGCCT+TGG 0.466903 7.2:-999264 None:intergenic
GGTGAATTGGTGACTGAAGT+TGG 0.470985 7.2:-999479 None:intergenic
CTGGAGAAAATATGGTCAAA+AGG 0.471109 7.2:+998421 MS.gene024451:CDS
GGAGAGACTAAGACCATTGA+TGG 0.480847 7.2:+997928 MS.gene024451:CDS
GACCATTGATGGTAAAATGA+AGG 0.490394 7.2:+997939 MS.gene024451:CDS
GGTTTGTCAGAATATCAATT+GGG 0.490887 7.2:+999392 MS.gene024451:CDS
TCTGATTCAATTGATGGAGA+AGG 0.500124 7.2:-999338 None:intergenic
ACAATGCTCTCTTCAAGAAA+AGG 0.501466 7.2:+998028 MS.gene024451:CDS
CTTTGGATGATGAATATAGC+TGG 0.515960 7.2:+998402 MS.gene024451:CDS
ATCAGAAAATGAGATAATGA+TGG 0.516212 7.2:+999355 MS.gene024451:CDS
AAGATATCCAAATCAAGGAT+TGG 0.520860 7.2:-999500 None:intergenic
TGATGTTAAAAGAAATGACT+TGG 0.526063 7.2:+999277 MS.gene024451:CDS
TGGATATCTTGCTTCAAAGA+GGG 0.528389 7.2:+999513 MS.gene024451:CDS
GAGCATTGTGGTTAAACTCC+TGG 0.533299 7.2:-998014 None:intergenic
GAATATCAATTGGGCTACAA+TGG 0.533841 7.2:+999401 MS.gene024451:CDS
TGAAGATCCAACAATAATTG+AGG 0.540253 7.2:+999088 MS.gene024451:CDS
AGAGACTAGTTTCACTTCCA+AGG 0.541625 7.2:+999247 MS.gene024451:CDS
GGATATCTTGCTTCAAAGAG+GGG 0.560641 7.2:+999514 MS.gene024451:CDS
TAGCAACAAAGCAGGTTCAA+AGG 0.565187 7.2:+999060 MS.gene024451:CDS
AAATATTCAGGAGTATTGAG+AGG 0.566440 7.2:-999554 None:intergenic
CATAGTAAGTGCTTTCTGGT+AGG 0.575281 7.2:-997890 None:intergenic
TTGGATATCTTGCTTCAAAG+AGG 0.575611 7.2:+999512 MS.gene024451:CDS
CAATGAATTAATCCAAGGAA+AGG 0.585373 7.2:+997786 MS.gene024451:CDS
CTTACTATGTTGAATGTGAG+AGG 0.591736 7.2:+997904 MS.gene024451:CDS
AATATTCAGGAGTATTGAGA+GGG 0.594267 7.2:-999553 None:intergenic
AAAGAGCATGCCTAAATGGA+AGG 0.601574 7.2:+998337 MS.gene024451:CDS
GTGAAGATTTGCACAAACAC+AGG 0.602509 7.2:+998365 MS.gene024451:CDS
ATAACCAATGAATTAATCCA+AGG 0.604601 7.2:+997781 MS.gene024451:CDS
GAGAAGGAAAACAAAACCGA+GGG 0.610739 7.2:-999322 None:intergenic
AGGGTGCCTAGCAACAAAGC+AGG 0.623925 7.2:+999052 MS.gene024451:CDS
ACAAACACAGGATTATCTGA+AGG 0.625241 7.2:+998377 MS.gene024451:CDS
AAGTGAGGTCATCATAGACC+AGG 0.627405 7.2:+997996 MS.gene024451:CDS
CAAACACAGGATTATCTGAA+GGG 0.645284 7.2:+998378 MS.gene024451:CDS
AAACGAGAGTCCCAAAAGTG+AGG 0.654222 7.2:+997981 MS.gene024451:CDS
ATAATTGAGGTAACTTACAG+AGG 0.655185 7.2:+999101 MS.gene024451:CDS
GGAGAAGGAAAACAAAACCG+AGG 0.666774 7.2:-999323 None:intergenic
GACTTGGAAGAAACCAAAGA+GGG 0.669176 7.2:+999293 MS.gene024451:CDS
ACTATGTTGAATGTGAGAGG+AGG 0.670945 7.2:+997907 MS.gene024451:CDS
TGACTTGGAAGAAACCAAAG+AGG 0.672282 7.2:+999292 MS.gene024451:CDS
TGCACACATAGAAATGGACA+AGG 0.697085 7.2:+999032 MS.gene024451:CDS
GCACACATAGAAATGGACAA+GGG 0.758330 7.2:+999033 MS.gene024451:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTTTTGTTAAATATTTA+AGG + chr7.2:998706-998725 MS.gene024451:intron 10.0%
!!! ATTAATAATATTTGTTTTAG+TGG + chr7.2:998531-998550 MS.gene024451:intron 10.0%
!! AAATGATACTTGTAATAAAA+CGG + chr7.2:998202-998221 MS.gene024451:intron 15.0%
!! CTATGTTAATAAAAAAGTTA+AGG - chr7.2:998128-998147 None:intergenic 15.0%
!!! AAATGTTTTCACAATTTTAT+TGG + chr7.2:998984-999003 MS.gene024451:intron 15.0%
!! CATTGGATGATATAATTATA+TGG - chr7.2:997834-997853 None:intergenic 20.0%
!! TATGTAATCATTTGAATTTG+TGG + chr7.2:998309-998328 MS.gene024451:intron 20.0%
!!! ATATTGCTCAACTATTTTTT+TGG + chr7.2:998667-998686 MS.gene024451:intron 20.0%
!!! TGCACTATTTGTTAATAATA+AGG + chr7.2:998776-998795 MS.gene024451:intron 20.0%
!!! TTCAACATTTGTATTGTTTT+CGG + chr7.2:998875-998894 MS.gene024451:intron 20.0%
!!! TTTTCACAATTTTATTGGTT+TGG + chr7.2:998989-999008 MS.gene024451:intron 20.0%
! ATAACCAATGAATTAATCCA+AGG + chr7.2:997781-997800 MS.gene024451:CDS 25.0%
! ATCAGAAAATGAGATAATGA+TGG + chr7.2:999355-999374 MS.gene024451:CDS 25.0%
! ATCTTGAGTAATGAAATAGA+AGG - chr7.2:998755-998774 None:intergenic 25.0%
! TAAGTTACCTCAATTATTGT+TGG - chr7.2:999098-999117 None:intergenic 25.0%
! TGATGTTAAAAGAAATGACT+TGG + chr7.2:999277-999296 MS.gene024451:CDS 25.0%
! TTTGTCAATCAAGAATTCAT+TGG - chr7.2:997860-997879 None:intergenic 25.0%
!! ATAGTTGAGCAATATTGATT+TGG - chr7.2:998662-998681 None:intergenic 25.0%
!! TCATTTTCTGATTCAATTGA+TGG - chr7.2:999347-999366 None:intergenic 25.0%
!!! AAGCGTTTTGAATTTGATAT+AGG + chr7.2:998569-998588 MS.gene024451:intron 25.0%
!!! ATGATGGAATCAAACTTTTT+TGG + chr7.2:999371-999390 MS.gene024451:CDS 25.0%
!!! CAATATTGATTTGGTTAACA+GGG - chr7.2:998653-998672 None:intergenic 25.0%
AAGATATCCAAATCAAGGAT+TGG - chr7.2:999503-999522 None:intergenic 30.0%
ATAATTGAGGTAACTTACAG+AGG + chr7.2:999101-999120 MS.gene024451:CDS 30.0%
CAATGAATTAATCCAAGGAA+AGG + chr7.2:997786-997805 MS.gene024451:CDS 30.0%
CCCTCAAAGTTAAAGATAAA+GGG + chr7.2:999173-999192 MS.gene024451:CDS 30.0%
CTTTCCTTGGATTAATTCAT+TGG - chr7.2:997788-997807 None:intergenic 30.0%
GAATATAGCTGGAGAAAATA+TGG + chr7.2:998413-998432 MS.gene024451:CDS 30.0%
GGTTTGTCAGAATATCAATT+GGG + chr7.2:999392-999411 MS.gene024451:CDS 30.0%
TATGGTCAAAAGGATATTCT+TGG + chr7.2:998431-998450 MS.gene024451:CDS 30.0%
TCCCTCAAAGTTAAAGATAA+AGG + chr7.2:999172-999191 MS.gene024451:CDS 30.0%
TGAAGATCCAACAATAATTG+AGG + chr7.2:999088-999107 MS.gene024451:CDS 30.0%
! AGAAGACAAAGTGCTTATTA+AGG + chr7.2:998825-998844 MS.gene024451:intron 30.0%
! ATCCTTCATTTTACCATCAA+TGG - chr7.2:997944-997963 None:intergenic 30.0%
! CCCTTTATCTTTAACTTTGA+GGG - chr7.2:999176-999195 None:intergenic 30.0%
! TCAAGCTAAGCCTTTAAAAA+GGG + chr7.2:999145-999164 MS.gene024451:CDS 30.0%
! TGGTTTGTCAGAATATCAAT+TGG + chr7.2:999391-999410 MS.gene024451:CDS 30.0%
!! AAATATTCAGGAGTATTGAG+AGG - chr7.2:999557-999576 None:intergenic 30.0%
!! AATATTCAGGAGTATTGAGA+GGG - chr7.2:999556-999575 None:intergenic 30.0%
!! CAAGAATTCATTGGTTTCAT+TGG - chr7.2:997851-997870 None:intergenic 30.0%
!! GCAATATTGATTTGGTTAAC+AGG - chr7.2:998654-998673 None:intergenic 30.0%
!! TACAATGGATTATGCCAAAA+TGG + chr7.2:999416-999435 MS.gene024451:CDS 30.0%
ACAAACACAGGATTATCTGA+AGG + chr7.2:998377-998396 MS.gene024451:CDS 35.0%
ACAATGCTCTCTTCAAGAAA+AGG + chr7.2:998028-998047 MS.gene024451:CDS 35.0%
ACTGAAATACGTTCAAGCTA+GGG - chr7.2:998290-998309 None:intergenic 35.0%
CAAACACAGGATTATCTGAA+GGG + chr7.2:998378-998397 MS.gene024451:CDS 35.0%
CAATTCACCAATCCTTGATT+TGG + chr7.2:999493-999512 MS.gene024451:CDS 35.0%
CTGGAGAAAATATGGTCAAA+AGG + chr7.2:998421-998440 MS.gene024451:CDS 35.0%
CTTACTATGTTGAATGTGAG+AGG + chr7.2:997904-997923 MS.gene024451:CDS 35.0%
CTTTGGATGATGAATATAGC+TGG + chr7.2:998402-998421 MS.gene024451:CDS 35.0%
GAAGCAAGATATCCAAATCA+AGG - chr7.2:999508-999527 None:intergenic 35.0%
GAATATCAATTGGGCTACAA+TGG + chr7.2:999401-999420 MS.gene024451:CDS 35.0%
TACAGATGCACACATAGAAA+TGG + chr7.2:999026-999045 MS.gene024451:CDS 35.0%
TACTGAAATACGTTCAAGCT+AGG - chr7.2:998291-998310 None:intergenic 35.0%
TCAACATAGTAAGTGCTTTC+TGG - chr7.2:997897-997916 None:intergenic 35.0%
TCTGATTCAATTGATGGAGA+AGG - chr7.2:999341-999360 None:intergenic 35.0%
TGGATATCTTGCTTCAAAGA+GGG + chr7.2:999513-999532 MS.gene024451:CDS 35.0%
TTCACTTGTTCCTTCCATTT+AGG - chr7.2:998350-998369 None:intergenic 35.0%
TTCATGTGTGTGAATTGTCA+AGG + chr7.2:998258-998277 MS.gene024451:intron 35.0%
TTGGATATCTTGCTTCAAAG+AGG + chr7.2:999512-999531 MS.gene024451:CDS 35.0%
TTTAACATCAAGTTCTGCCT+TGG - chr7.2:999267-999286 None:intergenic 35.0%
! CAAATCAAGGATTGGTGAAT+TGG - chr7.2:999495-999514 None:intergenic 35.0%
! CTCAAGCTAAGCCTTTAAAA+AGG + chr7.2:999144-999163 MS.gene024451:CDS 35.0%
! GACCATTGATGGTAAAATGA+AGG + chr7.2:997939-997958 MS.gene024451:CDS 35.0%
! GAGGGAAATTCCCTTTTTAA+AGG - chr7.2:999158-999177 None:intergenic 35.0%
! GCCCTTTATCTTTAACTTTG+AGG - chr7.2:999177-999196 None:intergenic 35.0%
! TTCTTGGAGCTAAGTTTTCA+AGG + chr7.2:998447-998466 MS.gene024451:CDS 35.0%
!! CTTTTTAAAGGCTTAGCTTG+AGG - chr7.2:999146-999165 None:intergenic 35.0%
!! GTGATATCAGATTCTGAAGT+TGG - chr7.2:999443-999462 None:intergenic 35.0%
!! TCTATGATGACCTCACTTTT+GGG - chr7.2:997994-998013 None:intergenic 35.0%
!! TTTATTGGTTTGGATTGCAG+AGG + chr7.2:998999-999018 MS.gene024451:intron 35.0%
!!! ATTCTGAAGTTGGTCCATTT+TGG - chr7.2:999433-999452 None:intergenic 35.0%
!!! TTTTCTTGAAGAGAGCATTG+TGG - chr7.2:998029-998048 None:intergenic 35.0%
ACTATGTTGAATGTGAGAGG+AGG + chr7.2:997907-997926 MS.gene024451:CDS 40.0%
AGGATTATCTGAAGGGTCTT+TGG + chr7.2:998385-998404 MS.gene024451:CDS 40.0%
GACTTGGAAGAAACCAAAGA+GGG + chr7.2:999293-999312 MS.gene024451:CDS 40.0%
GAGAAGGAAAACAAAACCGA+GGG - chr7.2:999325-999344 None:intergenic 40.0%
GCACACATAGAAATGGACAA+GGG + chr7.2:999033-999052 MS.gene024451:CDS 40.0%
GGACTCTCGTTTGTAAAAGA+AGG - chr7.2:997973-997992 None:intergenic 40.0%
GGATATCTTGCTTCAAAGAG+GGG + chr7.2:999514-999533 MS.gene024451:CDS 40.0%
GGTTAACAGGGCTAAAAACA+GGG - chr7.2:998641-998660 None:intergenic 40.0%
GTGAAGATTTGCACAAACAC+AGG + chr7.2:998365-998384 MS.gene024451:CDS 40.0%
TAGCAACAAAGCAGGTTCAA+AGG + chr7.2:999060-999079 MS.gene024451:CDS 40.0%
TGCACACATAGAAATGGACA+AGG + chr7.2:999032-999051 MS.gene024451:CDS 40.0%
TGGTTAACAGGGCTAAAAAC+AGG - chr7.2:998642-998661 None:intergenic 40.0%
TGTAAGCATCTTAGTGTCAC+AGG + chr7.2:998599-998618 MS.gene024451:intron 40.0%
TGTGTGAATTGTCAAGGTCA+AGG + chr7.2:998264-998283 MS.gene024451:intron 40.0%
! AAAGAGCATGCCTAAATGGA+AGG + chr7.2:998337-998356 MS.gene024451:CDS 40.0%
! CATAGTAAGTGCTTTCTGGT+AGG - chr7.2:997893-997912 None:intergenic 40.0%
! GTCTATGATGACCTCACTTT+TGG - chr7.2:997995-998014 None:intergenic 40.0%
! TGACTTGGAAGAAACCAAAG+AGG + chr7.2:999292-999311 MS.gene024451:CDS 40.0%
! TTTGAACCTGCTTTGTTGCT+AGG - chr7.2:999061-999080 None:intergenic 40.0%
!! AGAGACTAGTTTCACTTCCA+AGG + chr7.2:999247-999266 MS.gene024451:CDS 40.0%
AAACGAGAGTCCCAAAAGTG+AGG + chr7.2:997981-998000 MS.gene024451:CDS 45.0%
AAGTGAGGTCATCATAGACC+AGG + chr7.2:997996-998015 MS.gene024451:CDS 45.0%
GCTTTGCTAACTCCTTTCCT+TGG - chr7.2:997801-997820 None:intergenic 45.0%
GGAGAAGGAAAACAAAACCG+AGG - chr7.2:999326-999345 None:intergenic 45.0%
GGAGAGACTAAGACCATTGA+TGG + chr7.2:997928-997947 MS.gene024451:CDS 45.0%
! GAGCATTGTGGTTAAACTCC+TGG - chr7.2:998017-998036 None:intergenic 45.0%
! GCAGAAAGAGCATGCCTAAA+TGG + chr7.2:998333-998352 MS.gene024451:intron 45.0%
! GGTGAATTGGTGACTGAAGT+TGG - chr7.2:999482-999501 None:intergenic 45.0%
!!! CCAAAGAGGGTATTTTCCCT+CGG + chr7.2:999306-999325 MS.gene024451:CDS 45.0%
CCGAGGGAAAATACCCTCTT+TGG - chr7.2:999309-999328 None:intergenic 50.0%
! AGGGTGCCTAGCAACAAAGC+AGG + chr7.2:999052-999071 MS.gene024451:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 997748 999586 997748 ID=MS.gene024451
chr7.2 mRNA 997748 999586 997748 ID=MS.gene024451.t1;Parent=MS.gene024451
chr7.2 exon 997748 998049 997748 ID=MS.gene024451.t1.exon1;Parent=MS.gene024451.t1
chr7.2 CDS 997748 998049 997748 ID=cds.MS.gene024451.t1;Parent=MS.gene024451.t1
chr7.2 exon 998337 998468 998337 ID=MS.gene024451.t1.exon2;Parent=MS.gene024451.t1
chr7.2 CDS 998337 998468 998337 ID=cds.MS.gene024451.t1;Parent=MS.gene024451.t1
chr7.2 exon 999019 999586 999019 ID=MS.gene024451.t1.exon3;Parent=MS.gene024451.t1
chr7.2 CDS 999019 999586 999019 ID=cds.MS.gene024451.t1;Parent=MS.gene024451.t1
Gene Sequence

>MS.gene024451

ATGGAAGAGACTACAAGGAGAAAACGTGTTAGTATAACCAATGAATTAATCCAAGGAAAGGAGTTAGCAAAGCAGCTAAGTAACCATATAATTATATCATCCAATGAAACCAATGAATTCTTGATTGACAAAATAATTTCAACCTACCAGAAAGCACTTACTATGTTGAATGTGAGAGGAGGAGAGACTAAGACCATTGATGGTAAAATGAAGGATTCTCATCCTTCTTTTACAAACGAGAGTCCCAAAAGTGAGGTCATCATAGACCAGGAGTTTAACCACAATGCTCTCTTCAAGAAAAGGTTATGTCAAACTATTTTTTCTTGTAGATATCAATATATACAACTAATAGTTTTTTTTTTATTAAATACACATTACCTTAACTTTTTTATTAACATAGTTAGAACGTCAACTTATTCATAATGATGTCTTTGAACTCGTAATTTTGCTTAAAAAATGATACTTGTAATAAAACGGAGAGAATGTTGTTGTTTTGTAACTTTTGTTTCTTTCATGTGTGTGAATTGTCAAGGTCAAGGCCCTAGCTTGAACGTATTTCAGTATGTAATCATTTGAATTTGTGGTGCAGAAAGAGCATGCCTAAATGGAAGGAACAAGTGAAGATTTGCACAAACACAGGATTATCTGAAGGGTCTTTGGATGATGAATATAGCTGGAGAAAATATGGTCAAAAGGATATTCTTGGAGCTAAGTTTTCAAGGTTAGAAAGCTTTTTCTTCATTTCATTCAATTTTATTTGTTGTTATAAATTAAACAAGTAAAATTAATAATATTTGTTTTAGTGGAAAAGTTTGTACAATAAGCGTTTTGAATTTGATATAGGAATAACATGTAAGCATCTTAGTGTCACAGGTCTTATAATGTAAGTTCCCTGTTTTTAGCCCTGTTAACCAAATCAATATTGCTCAACTATTTTTTTGGTTACAAAATGATTATTACTTTTTTGTTAAATATTTAAGGCTGTTTACATTTTTGTGAAAATACCTTCTATTTCATTACTCAAGATTTGCACTATTTGTTAATAATAAGGATAAAAGAGACTTTTAACGAGAGTAAAGAAGACAAAGTGCTTATTAAGGATGACGTGAAATATTGAAAATAGTTATTTCAACATTTGTATTGTTTTCGGAAATGATAGACATTTGTTCAATTAAAGAGTCTCTTTTTTCTCGTTAACAAGTAAAACTAACTCAAATTATAAAAAATGAAAATAAAAAATGTTTTCACAATTTTATTGGTTTGGATTGCAGAGGATATTACAGATGCACACATAGAAATGGACAAGGGTGCCTAGCAACAAAGCAGGTTCAAAGGTCAGATGAAGATCCAACAATAATTGAGGTAACTTACAGAGGAAGACACACATGCACTCATCCTCAAGCTAAGCCTTTAAAAAGGGAATTTCCCTCAAAGTTAAAGATAAAGGGCTTAGAGAAAAATAAACTTCACAATGATAAAAAGAATCAACTACAAACTCAAGAGACTAGTTTCACTTCCAAGGCAGAACTTGATGTTAAAAGAAATGACTTGGAAGAAACCAAAGAGGGTATTTTCCCTCGGTTTTGTTTTCCTTCTCCATCAATTGAATCAGAAAATGAGATAATGATGGAATCAAACTTTTTTGGTTTGTCAGAATATCAATTGGGCTACAATGGATTATGCCAAAATGGACCAACTTCAGAATCTGATATCACTGATACAGTTTCAGCTCCAACTTCAGTCACCAATTCACCAATCCTTGATTTGGATATCTTGCTTCAAAGAGGGGATTTTGACACTGATTTCCCTCTCAATACTCCTGAATATTTCTCTTCATAA
Protein sequence

>MS.gene024451.t1

MEETTRRKRVSITNELIQGKELAKQLSNHIIISSNETNEFLIDKIISTYQKALTMLNVRGGETKTIDGKMKDSHPSFTNESPKSEVIIDQEFNHNALFKKRKSMPKWKEQVKICTNTGLSEGSLDDEYSWRKYGQKDILGAKFSRGYYRCTHRNGQGCLATKQVQRSDEDPTIIEVTYRGRHTCTHPQAKPLKREFPSKLKIKGLEKNKLHNDKKNQLQTQETSFTSKAELDVKRNDLEETKEGIFPRFCFPSPSIESENEIMMESNFFGLSEYQLGYNGLCQNGPTSESDITDTVSAPTSVTNSPILDLDILLQRGDFDTDFPLNTPEYFSS