Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024492.t1 | AES82726.1 | 96.2 | 529 | 16 | 2 | 1 | 529 | 1 | 525 | 1.20E-277 | 965.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024492.t1 | Q9SA25 | 37.8 | 286 | 176 | 1 | 238 | 521 | 378 | 663 | 2.6e-52 | 208.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024492.t1 | G7L302 | 96.2 | 529 | 16 | 2 | 1 | 529 | 1 | 525 | 8.4e-278 | 965.3 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024492.t1 | MTR_7g116650 | 96.030 | 529 | 17 | 2 | 1 | 529 | 1 | 525 | 0.0 | 1055 |
| MS.gene024492.t1 | MTR_6g007690 | 37.609 | 343 | 202 | 5 | 192 | 529 | 11 | 346 | 6.52e-69 | 226 |
| MS.gene024492.t1 | MTR_7g079350 | 37.716 | 289 | 176 | 3 | 238 | 523 | 350 | 637 | 4.47e-56 | 201 |
| MS.gene024492.t1 | MTR_5g018570 | 38.339 | 313 | 183 | 5 | 220 | 523 | 296 | 607 | 1.48e-55 | 198 |
| MS.gene024492.t1 | MTR_4g123880 | 37.847 | 288 | 174 | 3 | 236 | 519 | 165 | 451 | 1.71e-55 | 193 |
| MS.gene024492.t1 | MTR_4g123880 | 37.847 | 288 | 174 | 3 | 236 | 519 | 165 | 451 | 5.31e-55 | 193 |
| MS.gene024492.t1 | MTR_2g073640 | 35.244 | 349 | 212 | 5 | 178 | 519 | 600 | 941 | 5.56e-55 | 200 |
| MS.gene024492.t1 | MTR_7g079320 | 37.370 | 289 | 177 | 3 | 238 | 523 | 343 | 630 | 8.83e-55 | 196 |
| MS.gene024492.t1 | MTR_8g070910 | 33.235 | 340 | 203 | 6 | 202 | 521 | 214 | 549 | 2.77e-54 | 193 |
| MS.gene024492.t1 | MTR_6g083980 | 38.246 | 285 | 170 | 4 | 233 | 514 | 34 | 315 | 3.69e-54 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024492.t1 | AT1G16260 | 37.762 | 286 | 176 | 1 | 238 | 521 | 369 | 654 | 1.45e-56 | 201 |
| MS.gene024492.t1 | AT1G16260 | 37.762 | 286 | 176 | 1 | 238 | 521 | 378 | 663 | 1.52e-56 | 201 |
| MS.gene024492.t1 | AT1G16260 | 37.762 | 286 | 176 | 1 | 238 | 521 | 378 | 663 | 1.52e-56 | 201 |
| MS.gene024492.t1 | AT1G56720 | 38.889 | 288 | 171 | 3 | 236 | 519 | 165 | 451 | 3.62e-56 | 196 |
| MS.gene024492.t1 | AT1G56720 | 38.889 | 288 | 171 | 3 | 236 | 519 | 165 | 451 | 3.62e-56 | 196 |
| MS.gene024492.t1 | AT1G56720 | 38.889 | 288 | 171 | 3 | 236 | 519 | 165 | 451 | 3.62e-56 | 196 |
| MS.gene024492.t1 | AT1G56720 | 38.889 | 288 | 171 | 3 | 236 | 519 | 165 | 451 | 3.62e-56 | 196 |
| MS.gene024492.t1 | AT2G42960 | 38.062 | 289 | 176 | 1 | 224 | 509 | 157 | 445 | 3.12e-55 | 193 |
| MS.gene024492.t1 | AT2G42960 | 38.062 | 289 | 176 | 1 | 224 | 509 | 157 | 445 | 3.12e-55 | 193 |
| MS.gene024492.t1 | AT2G42960 | 38.062 | 289 | 176 | 1 | 224 | 509 | 157 | 445 | 3.12e-55 | 193 |
Find 108 sgRNAs with CRISPR-Local
Find 179 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAGGATATAACCAAAT+GGG | 0.446888 | 7.2:-1386470 | MS.gene024492:CDS |
| AAAAGATAAACCTTGGACGC+GGG | 0.536874 | 7.2:-1385297 | MS.gene024492:CDS |
| AAACAGATAGCTACTTGGTC+TGG | 0.466838 | 7.2:-1385373 | MS.gene024492:CDS |
| AAAGAGGATATAACCAAATG+GGG | 0.650234 | 7.2:-1386469 | MS.gene024492:CDS |
| AAAGGGACAAGTTGATAAGG+AGG | 0.638792 | 7.2:-1384312 | MS.gene024492:CDS |
| AAATGGGGCAGTGGCAGTGA+TGG | 0.530740 | 7.2:-1386454 | MS.gene024492:CDS |
| AACAGCCAAACAAGCAAACA+AGG | 0.585721 | 7.2:+1385137 | None:intergenic |
| AACAGCTAGTAAAGATAAAT+TGG | 0.245513 | 7.2:+1386231 | None:intergenic |
| AACCTTGGACGCGGGAGTGC+AGG | 0.467218 | 7.2:-1385289 | MS.gene024492:CDS |
| AACGCAAGAATTTGTCAGCA+AGG | 0.589922 | 7.2:+1385996 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCTTAAAAATGAGCCTA+TGG | + | chr7.2:1384703-1384722 | None:intergenic | 30.0% | |
| AAAGAGGATATAACCAAATG+GGG | - | chr7.2:1383866-1383885 | MS.gene024492:intron | 30.0% | |
| AATGAATTGTAGTACCTTTC+TGG | + | chr7.2:1385867-1385886 | None:intergenic | 30.0% | |
| AGTGAATGATTGATTGATCA+AGG | + | chr7.2:1385320-1385339 | None:intergenic | 30.0% | |
| ATCAATGAAGAAACTTGTGT+TGG | - | chr7.2:1384844-1384863 | MS.gene024492:intron | 30.0% | |
| ATGAACTACTGACACAATAT+AGG | - | chr7.2:1384444-1384463 | MS.gene024492:intron | 30.0% | |
| ATGTAGTTGTTGATGTTGTT+GGG | + | chr7.2:1386277-1386296 | None:intergenic | 30.0% | |
| CAAAAGAGGATATAACCAAA+TGG | - | chr7.2:1383864-1383883 | MS.gene024492:intron | 30.0% | |
| CTCTTGCCTACAAAAATTAA+TGG | + | chr7.2:1385954-1385973 | None:intergenic | 30.0% | |
| GAACCATGTCAACTTTAATA+CGG | + | chr7.2:1386458-1386477 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 1383839 | 1386518 | 1383839 | ID=MS.gene024492 |
| chr7.2 | mRNA | 1383839 | 1386518 | 1383839 | ID=MS.gene024492.t1;Parent=MS.gene024492 |
| chr7.2 | exon | 1385963 | 1386518 | 1385963 | ID=MS.gene024492.t1.exon1;Parent=MS.gene024492.t1 |
| chr7.2 | CDS | 1385963 | 1386518 | 1385963 | ID=cds.MS.gene024492.t1;Parent=MS.gene024492.t1 |
| chr7.2 | exon | 1385065 | 1385517 | 1385065 | ID=MS.gene024492.t1.exon2;Parent=MS.gene024492.t1 |
| chr7.2 | CDS | 1385065 | 1385517 | 1385065 | ID=cds.MS.gene024492.t1;Parent=MS.gene024492.t1 |
| chr7.2 | exon | 1384845 | 1384960 | 1384845 | ID=MS.gene024492.t1.exon3;Parent=MS.gene024492.t1 |
| chr7.2 | CDS | 1384845 | 1384960 | 1384845 | ID=cds.MS.gene024492.t1;Parent=MS.gene024492.t1 |
| chr7.2 | exon | 1384486 | 1384746 | 1384486 | ID=MS.gene024492.t1.exon4;Parent=MS.gene024492.t1 |
| chr7.2 | CDS | 1384486 | 1384746 | 1384486 | ID=cds.MS.gene024492.t1;Parent=MS.gene024492.t1 |
| chr7.2 | exon | 1384190 | 1384390 | 1384190 | ID=MS.gene024492.t1.exon5;Parent=MS.gene024492.t1 |
| chr7.2 | CDS | 1384190 | 1384390 | 1384190 | ID=cds.MS.gene024492.t1;Parent=MS.gene024492.t1 |
| chr7.2 | exon | 1383839 | 1383856 | 1383839 | ID=MS.gene024492.t1.exon6;Parent=MS.gene024492.t1 |
| chr7.2 | CDS | 1383839 | 1383856 | 1383839 | ID=cds.MS.gene024492.t1;Parent=MS.gene024492.t1 |
| Gene Sequence |
>MS.gene024492 ATGCCATTTGGTGAATGTGGGGGTGCAAAAGAGGATATAACCAAATGGGGCAGTGGCAGTGATGGTTTCCCAACTACTCTCTGCTGCAGAAATGCTCTGACCATTTTGTCAGATGCCATGGCCTCACAAGTCCGTAACACAAAAGGACAAATGTTCCTTTCTCAAGAACAATGGCAAAGTTGCAACCAATCATTTCATCCACAACCAGGGATGTCACTCTCTTCATGTGGCTTTGATAACTTTTACTTTGGTAGCTCTCAATGCTCCAATTTATCTTTACTAGCTGTTCAGAACTCGCAAAAGTACTCAGATGCATTGAATCAATGCAGCCACTTCGACCTTTCGTTTGATGAGTCGTGTGAGGATTGTACAACTGCAATATTGGATCTGAGAAATAGTTTGTATGACGCAACAGGGCCGGACAGCAATGATATTGAGAGAGCCATTTGCGGAGTAGCAGCTCTTGTTGCTGTTGCATTTGAGCAACAAAATGATCCTTTCCTTGCTGACAAATTCTTGCGTTGCTTGCCACCTCCAGCAGCAGTACATGACAAGAGTAAGATTATTATACCAATGTTATAAATCATACTTAGGGATGAGAGTCTATGAACTACTGACACAATATAGGGACAACGGACACGACACTAACATGTCTCCACTCACTGGAAATAATTTGAGAAAAGCGAATAATTGAATATAATATATGTGTTGGTATCAGATCATGACACGTGCCTGATACTACACACGCCTTCAATTGAATTTGTCGGTGCTACATAGGTGAAAATAGACTAGGTTAAACTTTGCCAGGCTGAACATGTCTTACTTTGAAAATGTTAGGCTTCGGTCTGTCCTATTACTCTGCCATAGGCTCATTTTTAAGGTTTGGTCTGACTTTTTGGTATGTCTGTTTAACAGTCTTTCATGATTTTTTTGACGAAAAACATACACCACTAATAAATAAACTAGAATGAAATTGAACAAATTTTGTTTTGTGAACCTAGAATCATCAATGAAGAAACTTGTGTTGGCAATGTTGGTGATGGCTATCTTAGCATTATTGATTATTGCTTTTCTGGTAAAATGTGTATCAAAGAAGAAGCCTGTTAAGAAGCACGTCCAGTTGAAACAGATAGCTACTTGGTCTGGACTGTACTGGTTCACAAAAACTGAAATTGAAAATGCCATGAATTTTGAAAATGAAAAGATAAACCTTGGACGCGGGAGTGCAGGTGAAGTGTTTAGAGGTGTTTTGCCAAGTGGTCAAGTTGTGGCCATCAAGCATTTGACAAAGAGTAATACCTCTAGTTCTGATTCTTTCACTCGAGAACTTGCTGGTCTTTCTAGACTTCGTCATCCAAACCTTGTTTGCTTGTTTGGCTGTTGTATGGAAGATGGCGAAAGATATCTCGTCTACGAGTTCTGTGCCAATGGAAATCTTGCTCAACATCTCTTGAGTAATGCTCTCTACTTTTCACCACCCTTGATCAATCAATCATTCACTATATTCCAATGTTAATGTAAACGATGATTTTATTTATGTGTGTGTGACTATGTTCAGGAAGAGACAGCCATTTGACATGGGAAGCAAGAGTGAAGATTTTGAGAGAATGTTCATTTGCGCTTAAGTATCTCCATCATCATATAGAGGGTTGTGTTGTCCATAGAGATATCAAGGTAAGTGTGATGAAGTTAATCTGGATTGGATTCCGCACTTAACTGGGAATGATTAGAAAATGTTTTTTTTAATGCCTTGCATTGATTTTATTGTTTAGCTTACAAACATTCTTTTGACTGAGAAATACGAAGCCAAACTGTCGGATTTTGGATTGTCAAGGATGATGGGGATGGAAGAGAGCAAGGTTTTTACTGATGTTAGAGGAACTATAGGTTACATGGATCCAGAGTACATGAGTAATGCCAAGTTAACTTGTGCCAGTGATGTATATAGTTTTGGAATTGTTGCTTTGCAAATTCTGTCAGGACAGAAAGTCATAGAGTTGGATCTTGATGCAAGAGATCAGCTCACCAGAAAGGTACTACAATTCATTCATTTACATTACATTAAATCACATTCGATTATATTGTATATAATTCAACTCAAGTATATATGCACCATTAATTTTTGTAGGCAAGAGATGTGAGTATGGGAAAGCGTCCATTGTCGGATTTTGTAGACTCGCGACTAAAGGGACAAGTTGATAAGGAGGACTTTGGAGCTATTTTACAGATTGCAGTACTTTGTGTTGCCAAATCAAGCACAGGTCGGCCACCAATTGAGGTTGTTTTTGACGAATTGGACAAAGTCTATAGGGACATTGAGGCGCGCAAGGTACTAGTACTACTAATAATAATTTGCACAAGCACATTAATTAGTGCTTATTGTAGTTTTGGGCCCACCAAGCTGACAGCAGTTTTCTATTTATGCAGAAGGCAACCCCAACAACATCAACAACTACATCAACATCAACATCAACATCTAGCTCTAACTGAGTCCTCGGCGAAATGGTCATATATGTGTGTATGAGTAACATTGAAGAAGTGGAACTGGAAATTGGAAGATGAATATGAACATTACAGTACATATAATATTAATTTTTCGGCATACAACTATCACAACCGTATTAAAGTTGACATGGTTCCCTTGTCTTTCGCTCGCTCGCAGATCTTAAGGTGTACCTAA |
| Protein sequence |
>MS.gene024492.t1 MPFGECGGAKEDITKWGSGSDGFPTTLCCRNALTILSDAMASQVRNTKGQMFLSQEQWQSCNQSFHPQPGMSLSSCGFDNFYFGSSQCSNLSLLAVQNSQKYSDALNQCSHFDLSFDESCEDCTTAILDLRNSLYDATGPDSNDIERAICGVAALVAVAFEQQNDPFLADKFLRCLPPPAAVHDKKSSMKKLVLAMLVMAILALLIIAFLVKCVSKKKPVKKHVQLKQIATWSGLYWFTKTEIENAMNFENEKINLGRGSAGEVFRGVLPSGQVVAIKHLTKSNTSSSDSFTRELAGLSRLRHPNLVCLFGCCMEDGERYLVYEFCANGNLAQHLLRRDSHLTWEARVKILRECSFALKYLHHHIEGCVVHRDIKLTNILLTEKYEAKLSDFGLSRMMGMEESKVFTDVRGTIGYMDPEYMSNAKLTCASDVYSFGIVALQILSGQKVIELDLDARDQLTRKARDVSMGKRPLSDFVDSRLKGQVDKEDFGAILQIAVLCVAKSSTGRPPIEVVFDELDKVYRDIEARKILRCT |
